Patent application number | Description | Published |
20080199930 | METHOD AND APPARATUS FOR THE RAPID DISRUPTION OF CELLS OR VIRUSES USING MICRO BEADS AND LASER - A method and apparatus for rapid disruption of cells or viruses using beads and a laser are provided. According to the method and apparatus for rapid disruption of cells or viruses using beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC. | 08-21-2008 |
20090005265 | METHOD OF ISOLATING NUCLEIC ACID USING MATERIAL POSITIVELY CHARGED AT FIRST PH AND CONTAINING AMINO GROUP AND CARBOXYL GROUP - A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex. | 01-01-2009 |
20090012302 | METHOD OF ISOLATING A NUCLEIC ACID USING A MATERIAL CONTAINING AN AMINO GROUP AND A CARBOXYL GROUP AND POSITIVELY CHARGED AT A FIRST PH AND A SOLID MATERIAL FOR NUCLEIC ACID ISOLATION USED FOR THE METHOD - Provided are a method of isolating a nucleic acid from a sample and a solid material for isolating the nucleic acid which can be used for the above method. The method includes contacting the sample with a bifunctional material containing an amino group and a carboxyl group at a first pH to bind the nucleic acid to the bifunctional material, the bifunctional material being positively charged at the first pH; and releasing the nucleic acid at a second pH which is higher than the first pH. | 01-08-2009 |
20090093380 | Method of manufacturing polymer array by coating photosensitizer - Provided is a method of manufacturing a polymer array by photolithography in which a molecule containing a photolabile protecting group is reacted with a surface of a substrate, and then a photosensitizer is coated on the surface of the substrate together with a coating material and the resulting substrate is exposed to light to perform a photochemical reaction. Even by using conventional semiconductor equipment and compounds without separately fabricating light exposure equipment or synthesizing a compound, a polymer array may be effectively manufactured by photolithography with lower exposure energy (shorter period of time). | 04-09-2009 |
20090153130 | FIELD EFFECT TRANSISTOR-BASED BIOSENSOR WITH INORGANIC FILM, METHOD OF MANUFACTURING THE BIOSENSOR, AND METHOD OF DETECTING BIOMOLECULE USING THE BIOSENSOR - Provided is a Field-Effect Transistor (FET)-based biosensor including: a substrate; a source and a drain, disposed on the substrate, having opposite polarity to the substrate; a gate, disposed on the substrate, contacting the source and the drain; and an inorganic film capable of binding with a biomolecule, disposed on a surface of the gate. A method of manufacturing the FET-based biosensor and a method of detecting a biomolecule using the FET-based biosensor is also provided. The FET-based biosensor can be manufactured using a semiconductor fabrication process without performing an additional process. Therefore, the inorganic film can be selectively deposited on a surface of a specific gate of a single FET, or on the surfaces of some gates of a plurality of FETs using patterning. Furthermore, the FET-based biosensor can be used to effectively detect trace amounts of a target biomolecule in a sample. | 06-18-2009 |
20090159463 | MICROFLUIDIC DEVICE COMPRISING ELECTROLYSIS DEVICE FOR CELL LYSIS AND METHOD FOR ELECTROCHEMICALLY LYSING CELLS USING THE SAME - Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same. | 06-25-2009 |
20090176899 | METHOD OF FORMING FILTER IN FLUID FLOW PATH IN MICROFLUIDIC DEVICE - A method for forming a filter in a fluid flow path in a microfluidic device is provided. The method includes introducing a photopolymerization reaction solution into the microfluidic device; and performing polymerization of photopolymerization reaction solution to form a filter in the fluid flow path in a microfluidic device. | 07-09-2009 |
20100261621 | MICROARRAY SUBSTRATE, METHOD OF USE, AND PRODUCTS COMPRISING THE MICROARRAY SUBSTRATE - Provided are a microarray substrate comprising a solid substrate coated with a chemical having a functional residue represented by Formula 1 or 2 below, a method of analyzing a biomolecule using the microarray substrate, and a lab-on-a-chip comprising the microarray substrate: | 10-14-2010 |
20120078527 | KIT AND METHOD FOR PREDICTING SENSITIVITY OF GASTRIC CANCER PATIENT TO ANTI-CANCER AGENT - A kit and method for predicting the sensitivity of gastric cancer patient to an anti-cancer agent are disclosed. | 03-29-2012 |
20130011931 | POLYMER INCLUDING GROUP HAVING AT LEAST TWO HYDROXYLS OR ZWITTERIONIC GROUP AND USE THEREOF - A solid support comprising a polymer that includes a group having at least two hydroxyls or a zwitterionic group and a method of using the same. | 01-10-2013 |
20130302822 | METHODS OF ANALYZING EXOSOMES USING FLUORESCENCE-LABELED EXOSOMES - A method of analyzing exosomes, the method comprising incubating a sample comprising fluorescence-labeled exosomes with a solid support so that the fluorescence-labeled exosomes bind to the solid support; measuring a fluorescence signal from the bound fluorescence-labeled exosomes; and analyzing the exosomes based on the measured fluorescence signal. | 11-14-2013 |
20130302856 | METHODS OF DIRECTLY EXTRACTING MICRORNA FROM MICROVESICLE IN CELL LINE, CELL CULTURE, OR BODY FLUID - A method of extracting a nucleic acid from a microvesicle, the method comprising treating the microvesicle with a composition comprising a detergent and an aprotic solvent to extract a nucleic acid from the microvesicle. | 11-14-2013 |
20140093880 | COMPOSITIONS, KITS, AND METHODS FOR ISOLATING VESICLES - A composition, a kit, and a method of isolating a vesicle from a sample using a compound comprising zwitterion moieties, which may be used to analyze vesicles, and proteins, glycoprotein, lipids, or nucleic acids thereof. | 04-03-2014 |
20140120629 | NUCLEIC ACID STRUCTURE COMPLEX INCLUDING NUCLEIC ACIDS, RAMAN-ACTIVE MOLECULES, AND METAL PARTICLES, METHOD OF PREPARING THE SAME, AND METHOD OF DETECTING TARGET MATERIAL BY USING THE NUCLEIC ACID STRUCTURE COMPLEX - Provided are nucleic acid structures suitable for reproducible Raman spectroscopy, methods of preparing the same, and methods of detecting a target material using the nucleic acid structures, whereby various target materials may be analyzed by using reproducible Raman spectroscopy. | 05-01-2014 |
20140162281 | COMPOSITIONS AND KITS FOR SEPARATING CELLS AND METHOD OF SEPARATING CELLS USING THE SAME - Provided are compositions, kits, and methods for separating cells including complexes of at least one type of linker capable of binding to an antibody or antigen binding fragment and a solid phase. | 06-12-2014 |
20150044725 | METHOD OF SEPARATING NUCLEIC ACIDS - A method of separating nucleic acids from cells, the method comprising incubating a sample comprising cells with a solid substrate that binds to the cells, whereby the cells adhere to the solid substrate; suspending the solid substrate adhered to the cells in a lysis composition comprising about 100 mM to about 300 mM of alkaline metal salt, and having a pH of about 6 to about 8; lysing the cells in the lysis composition to obtain a lysed solution; and obtaining the nucleic acids from the lysed solution; as well as related compositions and kits. | 02-12-2015 |