| Patent application number | Description | Published |
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| 20110111041 | BACTERIALLY DERIVED INTACT MINICELLS THAT ENCOMPASS PLASMID FREE FUNCTIONAL NUCLEIC ACID FOR IN VIVO DELIVERY TO MAMMALIAN CELLS - Intact, bacterially-derived minicells can safely introduce therapeutically effective amounts of plasmid-free functional nucleic acid to target mammalian cells. To this end, functional nucleic acid can be packaged into intact minicells directly, without resort to expression constructs, the expression machinery of the host cell, harsh chemicals or electroporation. | 05-12-2011 |
| 20110177185 | PHARMACEUTICALLY COMPATIBLE METHOD FOR PURIFYING INTACT BACTERIAL MINICELLS - The present invention provides a method for purifying bacterial minicells that involves subjecting a sample containing minicells to density gradient centrifugation in a biologically compatible medium. The method optionally includes a preliminary differential centrifugation step and one or more filtration steps. The invention also provides a method for purifying bacterial minicells in which a sample containing minicells is subjected to a condition that induces parent bacterial cells to adopt a filamentous form, followed by filtration of the sample to separate minicells from parent bacterial cells. The inventive methods optionally include one or more steps to remove endotoxin from purified minicell preparations, and/or treatment of purified minicell preparations with an antibiotic. Additionally, the invention provides purified minicell preparations, prepared according to the foregoing methods, and containing fewer than about 1 contaminating parent bacterial cell per 10 | 07-21-2011 |
| 20110275585 | TARGETED DELIVERY OF DRUGS, THERAPEUTIC NUCLEIC ACIDS AND FUNCTIONAL NUCLEIC ACIDS TO MAMMALIAN CELLS VIA INTACT KILLED BACTERIAL CELLS - A composition comprising intact killed bacterial cells that contain a therapeutic nucleic acid, a drug or a functional nucleic acid is useful for targeted delivery to mammalian cells. The targeted delivery optionally employs bispecific ligands, comprising a first arm that carries specificity for a killed bacterial cell surface structure and a second arm that carries specificity for a mammalian cell surface receptor, to target killed bacterial cells to specific mammalian cells and to cause endocytosis of the killed bacterial cells by the mammalian cells. Alternatively, the delivery method exploits the natural ability of phagocytic mammalian cells to engulf killed bacterial cells without the use of bispecific ligands. | 11-10-2011 |
| Patent application number | Description | Published |
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| 20090044828 | Methods and Systems for Debonding an Undesirable Material from a Device Using Ultrasonic Energy and Liquid Nitrogen - An undesirable material is at least partially debonded from a surface of a device at least partially immersed in a vessel containing subcooled liquid nitrogen by applying ultrasound energy with a sonicating member to the device via the subcooled liquid nitrogen. | 02-19-2009 |
| 20090047175 | Methods and Systems for Sanitizing or Sterilizing a Medical Device Using Ultrasonic Energy and Liquid Nitrogen - A medical device is sterilized or sanitized by at least partially submerging it within liquid nitrogen and imparting ultrasonic energy to it. | 02-19-2009 |
| 20100120082 | Optimization of Process Variables in Oxygen Enriched Fermentors Through Process Controls - Methods and systems are provided for controlling the addition of oxygen in fermentations to achieve a desired oxygen consumption and substrate yield in fermentation cell cultures. In one aspect, the invention provides a method for regulating the addition of oxygen (O | 05-13-2010 |
| 20110217691 | OXYGEN-ASSISTED FERMENTATION PROCESS - A fermentation process uses substantially pure oxygen. The oxygen is the only reactive gas which is injected into a fermentation vessel. The oxygen is moved through the vessel solely by its own pressure. The process can be used with both mechanically-agitated and air-lifted fermenters. The mechanically-agitated fermenter includes an analyzer for measuring oxygen concentration in the exhaust line, and adjusting the flow of fresh oxygen into the vessel accordingly. In the air-lifted fermenter, an analyzer measures the oxygen concentration in the head space of the vessel, and operates valves which either recycle the gas from the head space, or vent that gas to the outside, according to the measured concentration. A stream of nitrogen is periodically injected into the vessel to drive out carbon dioxide and other gases, to control the pH of the fermentation medium. The present invention substantially improves the efficiency of a commercial fermentation process. | 09-08-2011 |
