Patent application number | Description | Published |
20090317857 | Transformation of Algal Cells - Exemplary methods include a method for transforming an algal cell by preparing a transformation construct, preparing a particle for bombarding the algal cell, adhering the transformation construct to the particle, bombarding the algal cell with the particle, and growing the algal cell into a colony. The transformation construct is replicated within a nuclear genome of the algal cell and the growing of the algal cell is in a nutrient medium. Another exemplary method may include a method for genetically modifying an algal cell, by adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity, introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell, and selecting a colony that includes the transformed algal cell. | 12-24-2009 |
20090317904 | VCP-Based Vectors for Algal Cell Transformation - Provided herein are exemplary vectors for transforming algal cells. In exemplary embodiments, the vector comprises a Violaxanthin-chlorophyll a binding protein (Vcp) promoter driving expression of an antibiotic resistance gene in an algal cell. Embodiments of the invention may be used to introduce a gene (or genes) into the alga | 12-24-2009 |
20090325270 | USE OF 2-HYDROXY-5-OXOPROLINE IN CONJUNCTION WITH ALGAE - Provided herein are exemplary methods for the use of 2-hydroxy-5-oxoproline in conjunction with algae. One exemplary method includes applying an effective amount of 2-hydroxy-5-oxoproline to algae in an aqueous environment to accelerate creation of a high-cell density of the algae. The effective amount of the 2-hydroxy-5-oxoproline may be approximately 0.1 grams per liter of the aqueous environment, or up to approximately 0.1 grams per liter of the aqueous environment. The effective amount of the 2-hydroxy-5-oxoproline may be applied to the aqueous environment at or near a same time, or applied to the aqueous environment over a period of time. Exemplary algae cultivation systems are also provided herein. One exemplary system includes an aqueous environment having a pale-green mutant | 12-31-2009 |
20100210832 | BIDIRECTIONAL PROMOTERS IN NANNOCHLOROPSIS - Exemplary embodiments provided herein include novel promoters isolated from the microalgae, Nannochloropsis. These promoters drive gene expression in a bidirectional manner, and are especially useful for the genetic manipulation of Nannochloropsis and other organisms. The inventors herein successfully used these promoters (in both parallel and antiparallel orientations with respect to a Sh ble gene cassette) to impart zeocine-resistance to Nannochloropsis. | 08-19-2010 |
20100323387 | Optimization of Response to Light - Various aspects provide for exposing a substance to light. Certain aspects include exposing a suspension of photosynthetic organisms to sunlight, and may include optimizing exposure to improve photosynthesis conditions. Certain embodiments include controlling an opacity or opacity profile of a suspension of algae and/or diatoms. Optimizing exposure may include maximizing growth rate, maximizing photosynthesis efficiency, maximizing lipid production, minimizing damage, minimizing predator growth, maximizing a capacity to grow in suboptimal media (e.g., polluted water, brackish water, or water having a pH outside of a preferable range), minimizing requirements for nutrients, and other features. | 12-23-2010 |
20100330643 | Cyanobacterial Isolates Having Auto-Flocculation and Settling Properties - Provided herein are exemplary methods for production of biomass with a cyanobacterial isolate having auto-flocculation properties. One exemplary method includes isolating a cyanobacterial strain having a 16S ribosomal RNA sequence corresponding to SEQ. ID. NO. 1 herein, inoculating an algae cultivation system with the cyanobacterial strain, growing the cyanobacterial strain, and harvesting the biomass produced by the cyanobacterial strain. According to a further method, the harvesting of the biomass comprises ceasing agitation of the algae cultivation system, and pooling a slurry of the biomass produced by the cyanobacterial strain. In a further method, the harvesting of the biomass may comprise ceasing agitation within the algae cultivation system and/or allowing the biomass produced by the cyanobacterial strain to settle to near or at a bottom of the algae cultivation system. Also provided herein are exemplary cyanobacterial strains having flocculation properties for production of a biomass. | 12-30-2010 |
20110059495 | MANIPULATION OF AN ALTERNATIVE RESPIRATORY PATHWAY IN PHOTO-AUTOTROPHS - Exemplary methods for increasing TAG production in an algal cell during imbalanced growth conditions are provided. Some methods comprise knocking out an AOX gene, wherein the AOX gene produces an amino acid sequence having substantial similarity to the amino acid sequence of SEQ. ID. NO. 2. In further methods, the algal cell may be of genus | 03-10-2011 |
20110091977 | Homologous Recombination in an Algal Nuclear Genome - Exemplary transformation methods are provided for introducing deoxyribonucleic acid (DNA) into the nucleus of an algal cell. A transformation construct may be prepared, with the transformation construct having a first sequence of DNA similar to a corresponding first sequence of nuclear DNA, a second sequence of DNA similar to a corresponding second sequence of the nuclear DNA, and a sequence of DNA of interest inserted between the first and second sequences of DNA of the transformation construct. A target sequence of DNA inserted between the first and second corresponding sequences of the nuclear DNA may be transformed, resulting in replacement of the target sequence of DNA with the sequence of DNA of interest. Also provided are exemplary transformation constructs, with some transformation constructs having a first sequence of DNA similar to a corresponding first sequence of nuclear DNA of an algal cell, a second sequence of DNA similar to a corresponding second sequence of nuclear DNA of the algal cell, and a sequence of DNA of interest inserted between the first and second sequences of the transformation construct. | 04-21-2011 |
20110197306 | Efficient Light Harvesting - Various aspects provide for genetically modifying photosynthetic cells. In some cases, an integrated light harvesting efficiency of photosynthetic cells may be increased by reducing the amount of incident light that is absorbed but not used for photosynthesis. In some cases, an increased transparency may be associated with an increased light harvesting efficiency when absorption due to non-photosynthetic processes is reduced. A reduced capacity of various light-harvesting antenna apparatus may increase transparency. In some cases, a capacity of an organism to adapt to varying light levels may be reduced, and in certain cases, a modified organism may have a reduced ability to acclimate to a low light irradiance. | 08-11-2011 |
20120208279 | Transformation of Algal Cells - Exemplary methods include a method for transforming an algal cell by preparing a transformation construct, preparing a particle for bombarding the algal cell, adhering the transformation construct to the particle, bombarding the algal cell with the particle, and growing the algal cell into a colony. The transformation construct is replicated within a nuclear genome of the algal cell and the growing of the algal cell is in a nutrient medium. Another exemplary method may include a method for genetically modifying an algal cell, by adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity, introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell, and selecting a colony that includes the transformed algal cell. | 08-16-2012 |
20130078716 | VCP-Based Vectors for Algal Cell Transformation - Provided herein are exemplary vectors for transforming algal cells. In exemplary embodiments, the vector comprises a Violaxanthin-chlorophyll a binding protein (Vcp) promoter driving expression of an antibiotic resistance gene in an algal cell. Embodiments of the invention may be used to introduce a gene (or genes) into the alga | 03-28-2013 |
20130131330 | BIDIRECTIONAL PROMOTERS IN NANNOCHLOROPSIS - Exemplary embodiments provided herein include novel promoters isolated from the microalgae, | 05-23-2013 |
20130289262 | ACP Promoter - Isolated nucleotide sequences encoding a promoter of the Acyl Carrier Protein (“ACP”). | 10-31-2013 |