Patent application number | Description | Published |
20080220418 | Method For Providing Dna Fragments Derived From An Archived Sample - Aspects of the present invention relate to compositions and methods for providing DNA fragments from an archived sample (e.g., paraffin-embedded and/or fixed-tissue biopsies, etc). Particular aspects provide methods whereby high yields of DNA are isolated as well as a substantial portion of the DNA consists of long DNA fragments, and where the isolated genomic DNA is free of associated or cross-linked contaminants like proteins, peptides, amino acids or RNA. The methods are facile, cost-effective, an are characterized by high reproducibility and reliability. Particular aspects provide methods for providing DNA fragments derived from an archived sample, wherein the yield of DNA before, for example, an amplification step is at least 20%, and amplicons up to a length of about 1,000 base pairs are amplifiable. | 09-11-2008 |
20090263810 | BISULFITE CONVERSION OF DNA - The present invention provides an improved method for the bisulfite conversion of DNA, and facilitates the analysis of cytosine methylation of genomic DNA. Novel combinations of denaturing solvents, new reaction conditions and new purification methods provide surprisingly efficacious methods for bisulfite conversion of DNA relative to prior art methods. The converted DNA may subsequently be analyzed by many different methods. | 10-22-2009 |
20090280494 | Method for the detection of cytosine methylations in immobilized DNA samples - A method is described for the analysis of cytosine methylation patterns in genomic DNA samples. In the first method step, the genomic DNA is isolated from cells or other accompanying materials and bound essentially irreversibly to a surface. Then the DNA bound to the surface is treated, preferably with a bisulfite, in such a way that cytosine is converted into a base that is different in its base pairing behavior in the DNA duplex, while 5-methylcytosine remains unchanged. Then the reagents that were used are removed in a washing step. Finally, selected segments of the immobilized DNA are amplified in a polymerase reaction and the amplified products are investigated with respect to their sequence. | 11-12-2009 |
20100041024 | Bisulfite Conversion of DNA - The present invention relates to an improved method for the bisulfite conversion of DNA. In certain time-temperature ranges the efficacy of the bisulfite conversion is clearly improved. By combination with denaturating solvents, new reaction conditions and new purification methods the efficacy can be further increased The converted DNA can subsequently be analysed by different methods. The present invention facilitates the analysis of cytosine methylation. | 02-18-2010 |
20110059432 | METHOD FOR PROVIDING DNA FRAGMENTS DERIVED FROM A REMOTE SAMPLE - Aspects of the present invention relate to compositions and methods for providing DNA fragments from a remote sample. In particular aspects a remote sample comprising DNA is provided, DNA is isolated from the remote sample, and the isolated DNA is treated in a way which allows differentiation of methylated and unmethylated cytosine. Additional, particular embodiments provide compositions and methods for methylation analysis of DNA derived from a remote sample. Other aspects provide for compositions and methods of whole genome amplification of bisulfite treated DNA. | 03-10-2011 |
20110250601 | Bisulfite Conversion of DNA - The present invention provides an improved method for the bisulfite conversion of DNA, and facilitates the analysis of cytosine methylation of genomic DNA. Novel combinations of denaturing solvents, new reaction conditions and new purification methods provide surprisingly efficacious methods for bisulfite conversion of DNA relative to prior art methods. The converted DNA may subsequently be analyzed by many different methods. | 10-13-2011 |
20120258454 | Bisulfite conversion of DNA - The present invention relates to an improved method for the bisulfite conversion of DNA. In certain time-temperature ranges the efficacy of the bisulfite conversion is clearly improved. By combination with denaturating solvents, new reaction conditions and new purification methods the efficacy can be further increased The converted DNA can subsequently be analysed by different methods. The present invention facilitates the analysis of cytosine methylation. | 10-11-2012 |
20140087449 | Method for Providing DNA Fragments Derived from an Archived Sample - Aspects of the present invention relate to compositions and methods for providing DNA fragments from an archived sample (e.g., paraffin-embedded and/or fixed-tissue biopsies, etc.). Particular aspects provide methods whereby high yields of DNA are isolated as well as a substantial portion of the DNA consists of long DNA fragments, and where the isolated genomic DNA is free of associated or cross-linked contaminants like proteins, peptides, amino acids or RNA. The methods are facile, cost-effective, and are characterized by high reproducibility and reliability. Particular aspects provide methods for providing DNA fragments derived from an archived sample, wherein the yield of DNA before, for example, an amplification step is at least 20%, and amplicons up to a length of about 1,000 base pairs are amplifiable. | 03-27-2014 |
Patent application number | Description | Published |
20100004109 | CENTRIFUGE CUP - The invention relates to a centrifuge cup, which has a container and a cover. The cover has a lever including a guide element in each case on opposing edge areas thereof. The guide elements are able to be introduced into an associated guide path of the container by pivoting the lever. The guide elements are implemented in such a way that in a closed state of the cover with the container, a clamping force (F) having vertical force component (F | 01-07-2010 |
20130331253 | Centrifuge Drive Head For Releasably Connecting A Driving System To A Rotor Of A Centrifuge, A Set And A Centrifuge Comprising The Drive Head - The present invention relates a centrifuge drive head for releasably connecting a driving system to a rotor of a centrifuge. The centrifuge drive head comprises a base body mounted for rotation about an axis of rotation (R) and at least a first and a second coupling element, mounted on the base body for swinging outwardly about a swivel axis. The first and second coupling elements differ from each other. | 12-12-2013 |
20140010739 | CENTRIFUGE VESSEL ASSEMBLY - The present invention relates to a centrifuge vessel assembly for use in a centrifuge, comprising a centrifuge vessel for holding a sample to be centrifuged, an adapter holding the centrifuge vessel, and a holding means holding the adapter for attaching the centrifuge vessel assembly to a rotor of the centrifuge, said centrifuge vessel having a vessel body and a sealable opening through which the sample to be centrifuged can be filled into said vessel, said vessel body having a cross-sectional area and a vessel height, said vessel height being oriented at right angles to said cross-sectional area, wherein said cross-sectional area is in the form of an oval cross-sectional area. | 01-09-2014 |
20140113796 | CLOSABLE CENTRIFUGE CUP - A closable centrifuge cup, comprising a cup and a lid which can be placed on the cup edge, closes the cup opening and can be fastened under tension by means of at least one clamping element with its lid edge to the cup edge, characterized by at least one of the following properties:
| 04-24-2014 |