Patent application number | Description | Published |
20090191632 | SWELLABLE (METH)ACRYLATE SURFACES FOR CULTURING CELLS IN CHEMICALLY DEFINED MEDIA - Synthetic surfaces capable of supporting culture of undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a peptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes hydroxyethyl methacrylate, 2-carboxyehylacrylate, and tetra(ethylene glycol) dimethacrylate. The conjugated peptide may include an amino acid sequence of Xaa | 07-30-2009 |
20110183418 | Peptide-Polymer Cell Culture Articles and Methods of Making - Functionalized peptide monomers, peptides that have been functionalized to contain a polymerization moiety, are disclosed. The use of these functionalized peptide monomers to form peptide polymers which are useful as synthetic surfaces capable of supporting culture of cells in culture, particularly cells that will be used therapeutically, is also disclosed. Methods of making the surfaces and methods of using the surfaces are also disclosed. | 07-28-2011 |
20110207216 | Synthetic Peptide (Meth) Acrylate Microcarriers - A process for forming microcarriers includes contacting an initiator-conjugated microcarrier base with one or more monomers and activating the initiator to initiate polymerization and to graft a polymer from the base via the initiator or a remnant thereof. At least one of the monomers is conjugated to a polypeptide so that incorporation of the monomer into the forming polymer conjugates the polypeptide to the polymeric coating as it is formed in situ. | 08-25-2011 |
20110207219 | MODIFIED SUBSTRATES FOR PROTECTION OF PEPTIDE-IMMOBILIZED SURFACES FROM GAMMA RADIATION DEGRADATION - In the present specification and claims, three modified cell culture substrates are disclosed for protecting peptide mimetic surfaces used in cell culture technology. The methods are able to retain the functionality of the bioactive species conjugated to the surface. In particular, a vitronectin peptide fragment Ac-Lys-Gly-Gly-Pro-Gln-Val-Thr-Arg-Gly-Asp-Val-Phe-Thr-Met-Pro-NH2 was able to facilitate growth and proliferation of undifferentiated human embryonic stem cells after stabilization using 3 different modified cell culture substrates for of protection against gamma irradiation. The modified substrates disclosed in this invention are (i) covalent attachment of the antioxidant molecules (via blocking step); (ii) The use of coatings consisting of anti-oxidants and stabilizers comprising glycoprotiens, sugars, carbohydrates, poly(amino acids), peptides and hydrophilic polymers; and (iii) a third method wherein the same methionine contained in the bioactive sequence used to facilitate growth and proliferation of human embryonic stem cells was also used as a sacrificial coating to protect the same conjugated sequence against damage by gamma irradiation. | 08-25-2011 |
20120208266 | ENZYME CLEAVABLE CELL RELEASE POLYMERIC SURFACE - Disclosed herein are cell culture surfaces comprising a substrate and a polymer layer on the substrate where the polymer layer contains enzyme-cleavable amino acid sequences and cell adhesive peptide sequences. | 08-16-2012 |
20120208972 | POLYMERIZABLE CATONIC PEPTIDE MONOMERS AND POLYMERS - Disclosed herein are functionalized cationic peptide monomers: peptide chains of two or more positively charged amino acids chosen from lysine or arginine, or derivatives thereof, which are functionalized, meaning that they are bound to one or more polymerization moieties. The functionalized cationic peptide monomers can be described by the formula Z-Xaa | 08-16-2012 |
20120220720 | FUNCTIONALIZED CELL BINDING PEPTIDES AND CELL CULTURE ARTICLES - Synthetic surfaces capable of supporting culture of cells in culture, particularly cells that will be used therapeutically, are disclosed. The synthetic cell culture surfaces have a functionalized peptide, a peptide that has been functionalized to contain a polymerization moiety, and optionally a spacer, grafted to a hydrophilic polymeric base material. Methods of making the surfaces and methods of using the surfaces are also disclosed. | 08-30-2012 |
20130203165 | SWELLABLE (METH)ACRYLATE SURFACES FOR CULTURING CELLS IN CHEMICALLY DEFINED MEDIA - Synthetic surfaces capable of supporting culture of undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a peptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes hydroxyethyl methacrylate, 2-carboxyehylacrylate, and tetra(ethylene glycol)dimethacrylate. The conjugated peptide may include an amino acid sequence of Xaa | 08-08-2013 |
20150072132 | ELECTRICALLY ISOLATING POLYMER COMPOSITION - An electrode coating composition that includes at least one crosslinkable monomer; at least one hydrophobic monomer; and at least one dielectric constant enhancing agent selected from dielectric enhancing monomers, ferroelectric particulates, and electroactive polymers. Coatings including the polymer of compositions, and articles including electrically isolating layers are also disclosed. | 03-12-2015 |