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Aranha
Ian L. Aranha, Seattle, WA US
| Patent application number | Description | Published |
|---|---|---|
| 20080206812 | Methods for generating high titer helper-free preparations of released recombinant AAV vectors - This invention provides methods and compositions for producing high titer, substantially purified preparations of recombinant adeno-associated virus (AAV) that can be used as vectors for gene delivery. At the onset of vector production, AAV producer cells of this invention typically comprise one or more AAV packaging genes, an AAV vector comprising a heterologous (i.e. non-AAV) transgene of interest, and a helper virus such as an adenovirus. The AAV vector preparations produced are generally replication incompetent but are capable of mediating delivery of a transgene of interest (such as a therapeutic gene) to any of a wide variety of tissues and cells. The AAV vector preparations produced according to this invention are also substantially free of helper virus as well as helper viral and cellular proteins and other contaminants. The invention described herein provides methods of producing rAAV particles by culturing producer cells under conditions, such as temperature and pH, that promote release of virus. Also provided is a quantitative, high-throughput assay useful in the assessment of viral infectivity and replication, as well as in the screening of agent that affect viral infectivity and/or replication. | 08-28-2008 |
| 20100248355 | METHODS FOR GENERATING HIGH TITER HELPER-FREE PREPARATIONS OF RELEASED RECOMBINANT AAV VECTORS - This invention provides methods and compositions for producing high titer, substantially purified preparations of recombinant adeno-associated virus (AAV) that can be used as vectors for gene delivery. At the onset of vector production, AAV producer cells of this invention typically comprise one or more AAV packaging genes, an AAV vector comprising a heterologous (i.e. non-AAV) transgene of interest, and a helper virus such as an adenovirus. The AAV vector preparations produced are generally replication incompetent but are capable of mediating delivery of a transgene of interest (such as a therapeutic gene) to any of a wide variety of tissues and cells. The AAV vector preparations produced according to this invention are also substantially free of helper virus as well as helper viral and cellular proteins and other contaminants. The invention described herein provides methods of producing rAAV particles by culturing producer cells under conditions, such as temperature and pH, that promote release of virus. Also provided is a quantitative, high-throughput assay useful in the assessment of viral infectivity and replication, as well as in the screening of agent that affect viral infectivity and/or replication. | 09-30-2010 |
Rohan Aranha, San Francisco, CA US
| Patent application number | Description | Published |
|---|---|---|
| 20110071981 | AUTOMATED INTEGRATED HIGH AVAILABILITY OF THE IN-MEMORY DATABASE CACHE AND THE BACKEND ENTERPRISE DATABASE - A cluster manager is configured to manage a plurality of copies of a mid-tier database as a mid-tier database cluster. The cluster manager may concurrently manage a backend database system. The cluster manager is configured to monitor for and react to failures of mid-tier database nodes. The cluster manager may react to a mid-tier database failure by, for example, assigning a new active node, creating a new standby node, creating new copies of the mid-tier databases, implementing new replication or backup schemes, reassigning the node's virtual address to another node, or relocating applications that were directly linked to the mid-tier database to another host. Each node or an associated agent may configure the cluster manager to behave in this fashion during initialization, based on common cluster configuration information. Each copy of the mid-tier database may be, for example, a memory resident database. Thus, a node must reload the entire database into memory to recover a copy of the database. | 03-24-2011 |
Rohan Aranha, Redwood City, CA US
| Patent application number | Description | Published |
|---|---|---|
| 20080222159 | DATABASE SYSTEM WITH ACTIVE STANDBY AND NODES - A system includes an active node and a standby node and zero or more replica nodes. Each of the nodes includes a database system, such as an in-memory database system. Client updates applied to the active node are written through to the standby node, and the standby node writes the updates through to a primary database and updates the replica nodes. Commit ticket numbers tag entries in transaction logs and are used to facilitate recovery if either of the active node or the standby node fails. Updates applied to the primary database are autorefreshed to the active node and written through by the active node to the standby node which propagates the updates to the replica nodes. Bookmarks are used to track updated records of the primary database and are used to facilitate recovery if either of the active node or the standby node fails. | 09-11-2008 |