| Patent application number | Description | Published |
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| 20080305504 | Compositions, methods, and kits for assaying complement activation - The present invention provides a fluorogenic composition for assaying complement activation that comprises a substrate for C3 convertase that is linked to a first fluorophore and a second fluorophore, wherein the fluorescence of the first and second fluorophores are mutually substantially quenched when the two fluorophores are present at a distance less than the characteristic distance for the two fluorophores. In one embodiment, the fluorescence of the first fluorophore is substantially quenched by the second fluorophore, and the second fluorophore emits heat upon quenching. The present invention also provides a method for assaying complement activation, wherein the method includes the steps of incubating a biological sample with a polymer to provide a polymeric biological sample, followed by incubating the polymeric biological sample with the fluorogenic composition, and measuring the fluorescence. The method may optionally include the step of measuring the optical properties of the biological sample prior to the incubation steps. The present invention further provides a kit for assaying complement activation that comprises the fluorogenic composition disclosed herein. | 12-11-2008 |
| 20090155829 | HEME CHOLINE ESTERS AND USES THEREOF - Methods of activating an apo-peroxidase are provided. The methods include the steps of providing a solution comprising an apo-peroxidase and a heme choline ester, hydrolyzing the heme choline ester with a choline esterase, and converting the apo-peroxidase to active peroxidase. The methods disclosed herein also provide for detecting the presence of an analyte in a sample. The methods include providing a binder capable of specifically binding the analyte wherein the binder is attached to a solid surface, applying the sample to the solid surface under conditions that permit binder-analyte binding, adding a choline esterase-conjugated binder that binds to the analyte, adding an apo-peroxidase, a heme choline ester, a peroxide, and a peroxide-activated signal generator, and detecting the signal produced by the peroxide-activated signal generator. An associated kit and components are also provided. | 06-18-2009 |
| 20090236541 | System and Methods for Optical Imaging - The invention relates to imaging methods and systems. The systems may comprise a white light source configured to generate light in a first wavelength range, an excitation source configured to generate light at one or more wavelengths for exciting a fluorescent substance, a first detector configured to acquire reflectance image data that represents white light reflected from a subject, and a second detector configured to acquire fluorescence image data that represents fluorescence emissions from the subject. At least one of the one or more wavelengths generated by the excitation light source is within the first wavelength range of the white light source. The fluorescent substance may be, for example, a fluorescent dye that is injected into a patient before or during a surgery. The system may also include an image processing engine and a display. The image processing engine may receive the reflectance image data and the fluorescence image data and generate a merged image in which the fluorescence image data is superimposed on the reflectance image data. The display may be used by a surgeon, for example, to more effectively visualize the surgical site during surgery. | 09-24-2009 |
| 20090253163 | ITERATIVE STAINING OF BIOLOGICAL SAMPLES - Automated methods and devices that facilitate iterative staining of biological samples from imaging applications are provided. The methods include the steps of providing a small volume flow cell containing a biological sample, applying a stain to the biological sample, combining at least two precursor reagents to form an activated destaining agent and wherein the activated destaining agent decomposition rate is greater than or similar to the destaining reaction rate, and flowing the destaining agent over the biological sample at a flow rate that is greater than the decomposition rate of the activated destaining agent. The process of staining, combining and flowing may be iteratively repeated. Also disclosed herein are devices for iterative staining of biological samples comprising a flow cell, in fluid communication with a premixer, wherein the volume capacity of the premixer is smaller than about five times the volume capacity of the flow cell. | 10-08-2009 |
| 20100047925 | SEQUENTIAL ANALYSIS OF BIOLOGICAL SAMPLES - Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided. | 02-25-2010 |
| 20100104513 | METHOD AND SYSTEM FOR DYE ASSESSMENT - The present disclosure generally relates to systems and methods for identifying the boundaries of tumors and assessing quantitatively the ability of dyes to highlight a tumor's boundary. In accordance with these methods and systems, images are taken of subjects administered agents labeled with dyes. After accessing the images, tumors are selected and routines employed to both identify the boundaries of the tumors, as well as, to quantify various aspects of the tumor boundaries. From these quantifiable descriptors the performances of the various dyes to highlight the boundaries of tumors are evaluated. | 04-29-2010 |
| 20100120043 | SEQUENTIAL ANALYSIS OF BIOLOGICAL SAMPLES - Methods for detecting a plurality of targets in a biological sample are provided. The method comprises contacting the biological sample with a plurality of target-binding probes simultaneously to form a plurality of target-bound probes and observing the signals from the target-bound probes sequentially. An associated kit and device for detection of the plurality of targets are also provided. | 05-13-2010 |
| 20100143258 | TUMOR MARGIN IMAGING AGENTS - In one aspect, the present invention provides a functionalized monodisperse polylysine comprising a linear monodisperse polylysine chain comprising constituent lysine monomer residues containing appended C | 06-10-2010 |
| 20100151438 | METHODS AND KITS FOR ENHANCING SEDIMENTATION AND RECOVERY OF CELLS IN A SAMPLE - The methods and kits provide sedimentation-enhancing agents that significantly increase the efficiency of cell-sedimentation. They also increase the efficiency of blood cell separation methods and thereby increase the recovery of total nucleated cells. | 06-17-2010 |
| 20100159572 | DEVICE AND ASSOCIATED METHOD - A microfluidic device for detecting one or more molecules of interest comprising: a non-conductive substrate; wherein the non-conductive substrate is provided with a plurality of thermally active elements is provided. A method for selectively functionalizing a plurality of thermally active elements is also provided. | 06-24-2010 |
| 20100216159 | SWITCHABLE AFFINITY BINDERS - Methods and kits for binding and releasing biological targets, comprising, a binder comprising an environmentally reactive molecular switch that can switch between a high affinity state, to bind the target, to a low affinity state, to release the target. | 08-26-2010 |
| 20110053171 | METHOD AND APPARATUS FOR ANTIGEN RETRIEVAL PROCESS - The invention provides a method for antigen retrieval of a formaldehyde-fixed tissue sample comprising incubating a formaldehyde-fixed tissue sample in a first antigen retrieval solution at a temperature of greater than 90° C., transferring the tissue sample to a second antigen retrieval solution, and incubating the tissue sample in the second antigen retrieval solution at a temperature of greater than 90° C. The invention also provides a kit and sample delivery device for carrying out the method. | 03-03-2011 |
| 20110070596 | AGENTS AND METHODS FOR ANALYZING PROTEIN INTERACTIONS - Agents and methods for qualitative and quantitative analysis a protein complex or protein complexes using isotope-labeled symmetrical bifunctional crosslinkers and mass spectrometry are provided. Targeting moieties, cell permeability moieties, or affinity moieties, may be appended to the bifunctional crosslinkers. The isotope-labeled symmetrical bifunctional crosslinkers may be used in a kit or as a library. | 03-24-2011 |
| 20110092381 | DETECTION OF PLURALITY OF TARGETS IN BIOLOGICAL SAMPLES - Methods for detecting a plurality of targets in a biological sample are provided. The method comprises contacting the biological sample with a plurality of target-binding probes to form a plurality of target-bound probes, covalently attaching at least one of the target-bound probes to the biological sample, and observing the signals from the target-bound probes sequentially. An associated kit and device for detection of the plurality of targets are also provided. | 04-21-2011 |
