Patent application number | Description | Published |
20110151446 | CELL TREATMENT SOLUTION AND METHOD OF PREPARING STAINED CELL SUSPENSION FOR A MEASUREMENT OF NUCLEAR DNA BY FLOW CYTOMETRY - A cell treatment solution and a method that is used for preparing a stained cell suspension that is provided to a measurement of nuclear DNA by flow cytometry. The cell treatment solution may include a surfactant, RNase, and a fluorescent dye. The surfactant may include, for example, a non-ionic surfactant, a zwitterionic surfactant, an anionic surfactant, and/or a cationic surfactant. In one method of the invention, stained cell suspension that is provided to a measurement of nuclear DNA by flow cytometry is prepared. The method may include adding a tissue sample to a cell treatment solution including a surfactant, RNase, and fluorescent dye, disaggregating the tissue sample, and filtering the disaggregated tissue sample. Another method of the invention includes disaggregating a tissue sample, preparing cell suspension by filtering the disaggregated tissue sample, and adding a cell treatment solution including a surfactant, RNase, and fluorescent dye. | 06-23-2011 |
20130017129 | PIPETTE MEMBER AND CELL ISOLATION INSTRUMENTAANM Shioyama; TakahiroAACI TokyoAACO JPAAGP Shioyama; Takahiro Tokyo JPAANM Suzuki; AkaneAACI TokyoAACO JPAAGP Suzuki; Akane Tokyo JP - A pipette member to be attachable to a container capable of accommodating a tissue and a liquid, is used for isolating cells from the tissue by stirring, and includes: a cylinder member which includes a first opening that is to be accommodated in the container, and a second opening that is to be connected to a cell isolation apparatus, and in which a passage for communicating the first opening with the second opening is defined; and a filter member which is disposed inside the cylinder member, and which separates the passage into a first part that includes the first opening, and a second part that includes the second opening. | 01-17-2013 |
20130028813 | Cell Isolation Instrument - A cell isolation instrument includes: a first container which has an opening in an upper portion; a filtration member which defines at least a part of a lower portion of the first container; an isolation member which is movably housed in the first container to collide with a tissue, thereby isolating cells; and a second container which houses the first container in a manner that the first container can be taken out. | 01-31-2013 |
20130330248 | CELL ISOLATION APPARATUS - A cell isolation apparatus in which tissue and fluid that are accommodated in a container are pipetted by a pipette, and cells are isolated from the tissue, the cell isolation apparatus includes: a nozzle to which the pipette is attached; a pump which is connected to the nozzle; a controller which is configured to control an operation of the pump to cause air to be ejected from the nozzle or to be sucked into the nozzle, thereby causing the pipette to perform pipetting; and a condition inputting unit in which a user inputs a condition of control of the controller. | 12-12-2013 |
20130337501 | CELL ANALYZING APPARATUS AND CELL ANALYZING METHOD - A cell analyzing apparatus includes: a histogram acquirer which is configured to perform measurement of a number of nuclear stained cells, and which, by using a result of the measurement, is configured to acquire a histogram indicating a fluorescence intensity; and an analysis controller which is configured to apply frequency analysis on data of the histogram acquired by the histogram acquirer, and which is configured to determine existence/nonexistence of cancer cells based on a result of the frequency analysis. | 12-19-2013 |
20140024022 | CELL TREATMENT SOLUTION AND METHOD OF PREPARING STAINED CELL SUSPENSION FOR A MEASUREMENT OF NUCLEAR DNA BY FLOW CYTOMETRY - A cell treatment solution and a method that is used for preparing a stained cell suspension that is provided to a measurement of nuclear DNA by flow cytometry. The cell treatment solution may include a surfactant, RNase, and a fluorescent dye. The surfactant may include, for example, a non-ionic surfactant, a zwitterionic surfactant, an anionic surfactant, and/or a cationic surfactant. In one method of the presently disclosed subject matter, stained cell suspension that is provided to a measurement of nuclear DNA by flow cytometry is prepared. The method may include adding a tissue sample to a cell treatment solution including a surfactant, RNase, and fluorescent dye, disaggregating the tissue sample, and filtering the disaggregated tissue sample. Another method of the presently disclosed subject matter includes disaggregating a tissue sample, preparing cell suspension by filtering the disaggregated tissue sample, and adding a cell treatment solution including a surfactant, RNase, and fluorescent dye. | 01-23-2014 |
20140030728 | METHOD AND APPARATUS FOR ANALYZING CELLS - A method of analyzing cells, the method comprising: measuring a number of cells which are nuclear stained, in a to-be-determined tissue, and acquiring a histogram showing a fluorescence intensity based on a result of the measurement; analyzing the histogram, and acquiring data of predetermined parameters; comparing the data of the parameters with first thresholds predetermined for the parameters, to perform a first cancer determination for each of the parameters on the to-be-determined tissue; performing a scoring process for each of the parameters, on a result of the first cancer determination for each of the parameters, to calculate scores of the parameters; and combining the scores of the parameters with one another, thereby performing a second cancer determination on the to-be-determined tissue. | 01-30-2014 |
20140149740 | DETERMINATION METHOD FOR CRYPTOGRAPHIC ALGORITHM USED FOR SIGNATURE, VERIFICATION SERVER AND PROGRAM - On the basis of revocation information of a certificate, information of a certification authority and of the certificate issued by the certification authority from a terminal device, and information of a cryptographic algorithm, validity of the certificate from the terminal device is determined. If the certificate is valid, a validation result treating the certificate as valid is created, and using information of the cryptographic algorithm from the terminal device and information of the cryptographic algorithm used for the signature of the certification authority which has been imparted to the revocation information of the certificate, a selection list of cryptographic algorithms used for the response signature to impart to the verification result of the certificate is created to determine the cryptographic algorithm used for the response signature to impart the verification result of the certificate on the basis of the created list and the cryptographic algorithms capable of being accommodated by the verification server. | 05-29-2014 |
20150078649 | CELL ANALYZING APPARATUS AND CELL ANALYZING METHOD - A cell analyzing apparatus includes: a first histogram acquiring section which is configured to acquire a first histogram of fluorescence intensities by using a result of a measurement of a number of nuclear stained cells; a second histogram acquiring section which is configured to acquire a second histogram that is normalized based on a fluorescence intensity value indicating a maximum value of the first histogram; a determining section which is configured to determine whether cancer cells exist or not, based on one of the first histogram and the second histogram; and an outputting section which is configured to output a result of the determination performed by the determining section. | 03-19-2015 |