Patent application number | Description | Published |
20080302975 | SURFACE PLASMON ENHANCED FLUORESCENCE SENSOR - Exciting light is irradiated through a dielectric material block toward an interface between the dielectric material block and a metal film formed on one surface of the dielectric material block, such that total reflection conditions may be satisfied. An intensity of analysis fluorescence produced by an analysis fluorescent substance, which is contained in a sample and produces the analysis fluorescence by being excited by an evanescent wave oozing out from the interface when the exciting light impinges upon the interface, is normalized in accordance with an intensity of reference fluorescence produced by a reference fluorescent substance, which has been located at a position for excitation by the exciting light. | 12-11-2008 |
20090079978 | METHOD FOR DETECTING MOLECULAR ANALYSIS LIGHT, AND APPARATUS AND SAMPLE PLATE FOR USE WITH THE SAME - A molecular analysis light detection apparatus is formed by: a sample plate provided with an enhancing member which is disposed at a small predetermined area of a sample contact surface contacting with a sample containing a substance to be analyzed and which generates an enhancing field for enhancing light generated from the substance to be analyzed at the predetermined area relative to the light at other areas of the sample contact surface when predetermined excitation light is applied; an excitation-light applying optical system for applying the excitation light to an illumination area which contains the predetermined area of the sample contact surface provided with the enhancing member and which is larger than the predetermined area; and a signal detector unit for detecting fluctuation of the light, which is enhanced by the enhancing field, generated from the substance to be analyzed. | 03-26-2009 |
20090230308 | FLUORESCENCE DETECTING METHOD AND FLUORESCENCE DETECTING APPARATUS - Fluorescence detection utilizes surface plasmon. The intensity of scattered light, which is substantially proportionate to the intensity of an electric field enhancing field generated on a metal film, is employed, to normalize and correct the intensity of fluorescence emitted by fluorescent labels with respect to the intensity of the electric field enhancing field. | 09-17-2009 |
20090242802 | FLOURESCENCE DETECTING METHOD AND FLUORESCENCE DETECTING APPARATUS - An excitation light beam of a wavelength that excites fluorescent labels and a reference light beam having a wavelength longer than the excitation light beam are irradiated through a dielectric block toward an interface between the dielectric block and a metal film, to cause a first electric field enhancing field and a second electric field enhancing field on the upper surface of the metal film, in fluorescence detection that utilizes surface plasmon. The intensity of scattered light of the second electric field enhancing field, which is substantially proportionate to the intensity of the second electric field enhancing field, is employed to normalize and correct the intensity of fluorescence emitted by fluorescent labels with respect to the intensity of the first electric field enhancing field, based on the relationship between the intensities of the first and second electric field enhancing fields. | 10-01-2009 |
20090261269 | OPTICAL SIGNAL DETECTION METHOD, APPARATUS, SAMPLE CELL AND KIT - A sensor chip includes a dielectric plate and a sensor portion having a metal layer deposited on a predetermined area on the dielectric plate. A photo-reactable labeling-substance of an amount corresponding to the amount of a substance to be detected in a sample binds to the sensor portion by contacting the sample with the sensor portion. The amount of the substance to be detected is obtained by irradiating the predetermined area with excitation light and by detecting light output from the photo-reactable labeling-substance in an enhanced electric field that has been generated on the metal layer by irradiation with the excitation light. The photo-reactable labeling-substance includes a photo-reactable substance enclosed by a light transmissive material that transmits light output from the photo-reactable substance to prevent metal quenching that occurs when the photo-reactable substance is located close to the metal layer. | 10-22-2009 |
20100231915 | TOTAL REFLECTION ILLUMINATED SENSOR CHIP, METHOD FOR PRODUCING THE TOTAL REFLECTION ILLUMINATED SENSOR CHIP, AND SENSING METHOD USING THE TOTAL REFLECTION ILLUMINATED SENSOR CHIP - A total reflection illuminated sensor chip is employed in a detecting method for detecting a detection target substance including the steps of: supplying a sample that includes the detection target substance onto a metal film formed on a surface of a dielectric prism, irradiating a measuring light beam onto the interface between the dielectric prism and the metal film such that conditions for total reflection are satisfied at the interface, and utilizing evanescent waves which are generated due to the irradiation of the measuring light beam to detect the detection target substance. Polishing streaks in the region of a metal film formation surface of the dielectric film, on which the metal film is formed, have directional properties with respect to a single direction. | 09-16-2010 |
20130164861 | BIOLOGICAL MOLECULE DETECTING APPARATUS AND BIOLOGICAL MOLECULE DETECTING METHOD - A laser beam is emitted onto fluorescent molecules within a solution to orient the fluorescent molecules. The direction in which the laser beam is emitted is switched to switch the transition moment direction of the fluorescent particles to be parallel or perpendicular to the vibrating direction of linearly polarized excitation light. Thereby, the fluorescent molecules of free molecules and binding molecules can be switched between an excited and a non excited state. There is a difference in the speeds at which the orientation directions change for molecules which have undergone and molecules which have not undergone antigen antibody reactions. Therefore, the fluorescence contributed by fluorescent molecules associated with free molecules and the fluorescence contributed by fluorescent molecules associated with binding molecules can be calculated respectively, to measure the concentration of a detection target substance with high sensitivity. | 06-27-2013 |
20130217035 | BIOLOGICAL MOLECULE DETECTING APPARATUS AND BIOLOGICAL MOLECULE DETECTING METHOD - A configuration was adopted, in which the orientations of free molecules and binding molecules within a solution are switched by switching the vibration direction of orientation controlling light, thereby switching the amount of light emitted by each free molecule and each binding molecule. There is a difference in the amounts of time required for the orientations of the free molecules and the binding molecules to switch accompanying the switch in the emission direction of the orientation controlling light. Therefore, the timings at which the amounts of light emitted by each type of molecule increase differ. Accordingly, the fluorescence contributed by fluorescent molecules associated with free molecules and the fluorescent molecules associated with binding molecules can be respectively calculated, even if all of the fluorescent molecules within the solution emit fluorescence. Thereby, the concentration of a detection target substance can be accurately measured with a simple structure. | 08-22-2013 |
20130224763 | BIOLOGICAL MOLECULE DETECTING APPARATUS AND BIOLOGICAL MOLECULE DETECTING METHOD - A biological molecule detecting apparatus capable of highly sensitive measurements is provided. A laser was emitted onto a solution, to impart external force onto free molecules and binding molecules within the solution. The external force inhibited Brownian motion of the free molecules and the binding molecules. The concentration of a detection target substance which is associated the binding molecules can be measured with high sensitivity, by measuring the Brownian motion of the free molecules and the binding molecules within the solution irradiated with the laser. | 08-29-2013 |
20130224764 | BIOLOGICAL MOLECULE DETECTING APPARATUS AND BIOLOGICAL MOLECULE DETECTING METHOD - A biological molecule detecting apparatus capable of highly sensitive measurements is provided. The emission direction of an orientation controlling light beam was periodically switched, to periodically switch the orientation direction of binding molecules | 08-29-2013 |
20130266480 | ASSAY CHIP - An assay chip includes fluidic-channel member composed of a light-transmissive lower member and an upper member, forming a fluidic-channel therebetween, and a cover member fitted with the fluidic-channel member from the upper-member-side thereof. An inlet for injecting a sample solution into the fluidic-channel and a suction opening for sucking, from the downstream side, the injected sample solution, both communicating with the fluidic-channel, are formed on the upper surface of the upper member. A pot for carrying out predetermined pre-processing on the sample solution, a pot for first-reaction processing to bind a photoresponsive labeling substance to an analyte in the sample solution, an inlet insertion-hole for inserting the inlet, and a suction-opening insertion-hole for inserting the suction opening are linearly arranged on the upper surface of the cover member. | 10-10-2013 |
20130295555 | BIO-MOLECULE DETECTING DEVICE AND BIO-MOLECULE DETECTING METHOD - A bio-molecule detecting device that enables high-sensitivity measurement is provided. The bio-molecule detecting device was configured so that the orientation directions of free molecules and binding molecules in a solution are switched by switching the radiation direction of orientation control light, whereby the light extinction amount can be changed using nanorods associated with the free molecules and the binding molecules. In addition, between the free molecules and the binding molecules, there is a difference in the necessary time for the orientation direction to be switched by switching the radiation direction of the orientation control light, and therefore the timings, at which the light extinction amounts by the respective molecules change, are different. | 11-07-2013 |
20140017810 | BIO-MOLECULE DETECTING DEVICE AND BIO-MOLECULE DETECTING METHOD - A bio-molecule detecting device that enables a high-sensitivity measurement is provided. The orientation direction of third complexes included in blood plasma is switched by switching the vibration direction of an orientation control light. The orientation direction of the third complexes is switched between two directions in which the intensities of an electric field, which is generated between two gold nanoparticles included in the third complexes by surface plasmon resonance, are significantly different. Therefore, the intensity of fluorescence generated from the third complexes is significantly changed by the change in the orientation direction of the third complexes. | 01-16-2014 |
Patent application number | Description | Published |
20090103090 | SENSING APPARATUS - The sensing apparatus includes a measuring light emission device, a waveguide member including a sensing surface modified by a surface modification substance, a detection device and an analyzer. The measuring light of a predetermined polarized state is emitted from the emission device so that the measuring light is totally reflected on the sensing surface of the waveguide member holding target substances labeled by fine metal particles to illuminate the particles by evanescent light generated near the sensing surface. The amount of evanescent light scattered by the fine metal particles is detected by the detection device. The measuring light emission device, the waveguide member and the analyzer are included in an optical waveguide system which sets a polarized state of scattered light generated when no target substance is present on the sensing surface in a crossed nicol relation to the analyzer. | 04-23-2009 |
20090218496 | SENSING APPARATUS AND A METHOD OF DETECTING SUBSTANCES - A sensing apparatus comprises: a prism; a metal film provided on a surface of the prism and which has provided on its surface a material that binds to an analyte; a substrate that is provided on a surface of the prism and which has formed therein a channel for supplying a liquid sample to the metal film; a light source for issuing light; an optical unit for incident light by which the light issued from the light source is launched into the prism at a specified angle; a light detecting unit for detecting as a first detection signal the light being generated in neighborhood of the metal film before the liquid sample is supplied and for detecting as a second detection signal the light being generated in neighborhood of the metal film that has become dry after the liquid sample is supplied; and an analyte detection unit for detecting the analyte contained in the liquid sample based on a difference between the first and the second detection signals detected by the light detecting unit. | 09-03-2009 |
20090218499 | SENSING APPARATUS - A sensing apparatus comprises: a prism; a metal film provided on a surface of the prism; a substrate that is provided on a surface of the prism and which has formed therein a channel for supplying a sample to the metal film; a light source for issuing light; an optical unit for incident light by which the light being issued from the light source is launched into the prism at such an angle that the light is totally reflected on a boundary surface between the prism and the metal film, the optical unit for incident light including a light intensity distribution adjusting section that reduces difference between the maximum and the minimum values in the intensity distribution of the light that is launched into the prism; and a light detecting unit for detecting the light that is generated in neighborhood of the metal film. | 09-03-2009 |
20090221089 | PROBE CHIP, SENSING APPARATUS USING THE SAME AND METHOD OF DETECTING SUBSTANCES USING THE SAME - A probe chip comprises: a prism; a metal film provided on a surface of the prism and which has provided on its surface a first binding material that binds to the analyte; and a channel substrate that is provided on a side of the prism and which has formed therein a channel for supplying the liquid sample to the metal film by causing the liquid material to travel from a beginning end portion to a terminal end portion, the channel being formed in such a way that a zone from a point between the beginning end portion and the metal film to a position of contact with the metal film separates into a first branch and a second branch that has an area where a second binding material that is labeled with the fluorescent material and which binds to the analyte is placed. | 09-03-2009 |
20100068824 | SENSING METHOD, SENSING DEVICE, INSPECTION CHIP, AND INSPECTION KIT - A sensing method comprises the steps of: allowing a liquid sample containing an analyte to flow through a channel, applying a force oriented in a given direction normal to a direction in which the liquid sample flows in the channel upon the analyte in a given position of the channel to move the analyte in the given direction so that the analyte is concentrated, causing the liquid sample to flow to a sensing surface forming a part of a wall surface of the channel located downstream of the given position and in the given direction against the channel, the sensing surface securing thereon a binding substance specifically reacting with the analyte, to allow the concentrated analyte to bind to the binding substance, and detecting a quantity of the analyte bound to the binding substance. | 03-18-2010 |