Wehrman, US
Edward Wehrman, Dearborn, MI US
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20140129339 | Method and Apparatus for Digital Coupon Presentation - A system includes a processor configured to receive a plurality of media elements including at least one media element containing an interactive advertisement. The processor is further configured to present the media elements to a vehicle occupant. The processor is also configured to receive acquiescence to store a coupon associated with at least one interactive advertisement. Additionally, the processor is configured to instruct storage of the coupon and instruct storage of any events associated with the coupon which may signal a reason to re-present the coupon to a driver. | 05-08-2014 |
Edward Wehrman, Leesburg, VA US
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20150293509 | IN-VEHICLE HOME AUTOMATION INTEGRATION - A nomadic device may be configured to connect to a vehicle computing system over a local connection and to an automation service over a wide-area connection, receive a message from the vehicle computing system over the local connection requesting a home automation action to be performed, and responsive to the message, send a command over the wide-area connection to an automation service to request the home automation action. A vehicle controller may be configured to connect to a nomadic device over a local connection, the nomadic device configured to connect to an automation service over a wide-area connection, receive input to a user interface of the vehicle computing system requesting performance of a home automation action of the automation service, and responsive to the input, send a command over the local connection to request the home automation action. | 10-15-2015 |
Geoffrey Wehrman, Minneapolis, MN US
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20120059854 | RELOCATION OF METADATA SERVER WITH OUTSTANDING DMAPI REQUESTS - A cluster of computer system nodes share direct read/write access to storage devices via a storage area network using a cluster filesystem and operating system implementing DMAPI. Threads executing on a metadata client know when a DMAPI event is required, and generate the DMAPI event on their own initiative when necessary. A metadata server maintains DMAPI queues. If the metadata server relocates to another host, the DMAPI events in the DMAPI queues are moved transparently to users. | 03-08-2012 |
Geoffrey Ray Wehrman, Eagan, MN US
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20140279913 | FILE SYSTEM REPLICATION - A data backup system for data and metadata is stored in a file storage system. Data in the file system is stored in a remote low priority data storage system, such as a remote tape storage system, power-managed array of secondary storage disks (MAID), or other lower cost secondary data storage system. Metadata for the file system is stored in a remote higher priority system, such as a remote disk storage system. As such, the data and metadata for a file storage system maintained at a primary location is backed-up in different types of storage systems at a remote location. By storing the data and metadata in different types of storage, the data can be backed up at less cost. In case of a disaster at the primary storage location, a user accessing data from a local server may be switched to access the data and the metadata from the remote server. | 09-18-2014 |
Matthew L. Wehrman, Cincinnati, OH US
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20150205936 | Technologies for Prescription Management - Technologies for managing a drug prescription may include determining submission requirements to submit a prescription of a user to an insurance payer for payment based on an identity of the insurance payer, generating a prescription payment submission form customized for the insurance payer based on the submission requirements, populating the prescription payment submission form with prescription information of the prescription and identification information of the user, and submitting the populated prescription payment submission form to an insurance payer processor server. Additionally, the disclosed technologies include receiving prescription co-pay information related to the prescription in response to submitting the prescription payment submission form and submitting an adjustment to the insurance payer processor server to cancel the submission of the prescription payment submission form. The disclosed technologies also include prior authorization technologies for determining a requirement of prior authorization and obtaining the prior authorization. Additionally, patient assistance technologies are disclosed. | 07-23-2015 |
Robert W. Wehrman, Glendale, MO US
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20080319868 | CLIENT CUSTOMIZED CARD FOR USE WITH SELECTED MERCHANTS - A client selects several merchants with whom the card may be redeemed, the amount redeemable, and a design for printing on the card which is personalized for the card recipient. | 12-25-2008 |
20110178924 | CLIENT CUSTOMIZED VIRTUAL OR PHYSICAL CARD FOR USE WITH SELECTED MERCHANTS - A client selects several merchants with whom the card may be redeemed, the amount redeemable, and a design for the card, which is personalized for the card recipient. | 07-21-2011 |
20130290181 | CLIENT CUSTOMIZED VIRTUAL OR PHYSICAL CARD FOR USE WITH SELECTED MERCHANTS - A client selects several merchants with whom the card may be redeemed, the amount redeemable, and a design for the card, which is personalized for the card recipient. | 10-31-2013 |
20150186873 | Client Customized Virtual or Physical Card for Use with Selected Merchants - A client selects several merchants with whom the card may be redeemed, the amount redeemable, and a design for the card, which is personalized for the card recipient. | 07-02-2015 |
20150227919 | Client Customized Virtual or Physical Card for Use with Selected Merchants - A client selects several merchants with whom the card may be redeemed, the amount redeemable, and a design for the card, which is personalized for the card recipient. | 08-13-2015 |
Susan L. Wehrman, Ballwin, MO US
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20120296676 | TRANSFORMING DATA FOR RENDERING AN INSURABILITY DECISION - Transformation of disparate data for use in rendering a decision involving a potentially insurable risk. An Extract, Transform, Load (ETL) process extracts the data and converts it from a plurality of formats into a standard format for processing. A heuristic engine inferentially processes the converted data to identify information relevant to the decision to be rendered. A consolidation and presentation engine generates presentable knowledge from the relevant information and then presents the knowledge to a decision-making entity for rendering the decision. And an optimization feedback process monitors one or more actions on the presented knowledge by the decision-making entity and adjusts one or more of the ETL process, the heuristic engine, and the consolidation and presentation engine as a function of the monitored actions. | 11-22-2012 |
20140278588 | TRANSFORMING DATA FOR RENDERING AN INSURABILITY DECISION - Transformation of disparate data for use in rendering a decision involving a potentially insurable risk. An Extract, Transform, Load (ETL) process extracts the data and converts it from a plurality of formats into a standard format for processing. A heuristic engine inferentially processes the converted data to identify information relevant to the decision to be rendered. A consolidation and presentation engine generates presentable knowledge from the relevant information and then presents the knowledge to a decision-making entity for rendering the decision. And an optimization feedback process monitors one or more actions on the presented knowledge by the decision-making entity and adjusts one or more of the ETL process, the heuristic engine, and the consolidation and presentation engine as a function of the monitored actions. | 09-18-2014 |
Thomas Wehrman, East Palo Alto, CA US
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20100041052 | Receptor Tyrosine Kinase Assays - Methods for detecting phosphorylation of receptor tyrosine kinases (“RTKs”) upon activation are provided. The method employs cells comprising two fusion products: (1) an RTK fused to a small fragment of β-galactosidase and (2) a phosphotyrosine binding peptide fused to the large fragment of β-galactosidase, where the 2 fragments weakly complex to form an active enzyme, and optionally a construct for a cytosolic RTK phosphorylating kinase, when the RTK does not autophosphoryate. To detect phosphorylation a β-galactosidase substrate is added to the cells, whereby product formation indicates the occurrence of phosphorylation. | 02-18-2010 |
Thomas Wehrman, Mountain View, CA US
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20120040372 | RECEPTOR TYROSINE KINASE ASSAYS - Methods for detecting phosphorylation of receptor tyrosine kinases (“RTKs”) upon activation and the modulation of activation by a candidate compound are provided. The method employs cells comprising two fusion products: (1) an RTK fused to a small fragment of β-galactosidase and (2) a phosphotyrosine binding peptide fused to the large fragment of β-galactosidase, where the 2 fragments weakly complex to form an active enzyme, and optionally a construct for a cytosolic RTK phosphorylating kinase, when the RTK does not autophosphoryate. To detect phosphorylation a β-galactosidase substrate is added to the cells, whereby product formation indicates the occurrence of phosphorylation. | 02-16-2012 |
Thomas S. Wehrman, Menlo Park, CA US
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20140045194 | MONITORING PROTEIN TRAFFICKING USING BETA-GALACTOSIDASE REPORTER FRAGMENT COMPLEMENTATION - Methods and materials are disclosed for use in an enzyme fragment complementation assay using complementary fragments of β-galactosidase to study the trafficking of proteins in a cell. Compounds that bind to a target peptide have been found to affect protein folding and therefore trafficking. β-Galactosidase fragments, an enzyme donor (ED) and an enzyme acceptor (EA), are fused to a target peptide and to an intracellular compartment protein, wherein the compartment is involved in intracellular trafficking. Contacting the cell with a compound that binds to the target peptide results in enhanced movement of the protein through the cellular trafficking pathway comprised of the endoplasmic reticulum, Golgi apparatus, the plasma membrane, endosomes, etc. Using this approach, compounds that bind to a target peptide and alter its ability to traffic through the normal cellular pathway can be readily detected. | 02-13-2014 |
20140370522 | DETECTION OF PROTEIN TRANSLOCATION BY BETA-GALACTOSIDASE REPORTER FRAGMENT COMPLEMENTATION - Methods and compositions are provided for detecting molecular translocations, particularly protein translocations within and between sub-cellular compartments, using at least two components that exhibit a localization-dependent difference in complementation activity. In particular, alpha-complementing β-galactosidase fragments are provided. These β-galactosidase reporter fragments display significantly enhanced enzymatic activity when one fragment is localized in a membrane. Methods for carrying out no-wash ELISA assays based on the reporter component system are also provided. | 12-18-2014 |
Thomas S. Wehrman, Mountain View, CA US
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20100120063 | GPCR Arrestin Assays - Sensitive assays for candidate compounds affecting GPCR activity are provided using a cell containing fusion proteins comprising a first fusion protein comprising (a) a target GPCR fused to a small fragment of β-galactosidase through a linker comprising a phosphorylation site or (b) a GPCR or a protein of interest, where the GPCR and protein of interest form a complex and one of them is fused to the small fragment of β-galactosidase; and a second fusion protein comprising arrestin fused to a large fragment of β-galactosidase. In (a), the affinity of the small and large fragments is optimized based on the background to signal ratio and the absolute signal observed. The assay is performed using a β-galactosidase substrate that provides a detectable optical signal. | 05-13-2010 |
20100151496 | ASSAYS FOR NUCLEAR HORMONE RECEPTOR BINDING - Methods and genetic constructs are provided for detecting the binding of nuclear hormone receptors to a coactivator/corepressor. The methods employ enzyme fragment complementation using fragments of β-galactosidase as the detection system. Cells are transformed to express the large fragment of β-galactosidase fused to a member of the complex with NHR for initiation of transcription and have it localized in the nucleus and to express the small fragment of β-galactosidase fused to the nuclear hormone receptor for binding to the member upon stimulation with a ligand. | 06-17-2010 |
20100203555 | Wild-Type Receptor Assays - A method for determining ligand activation of receptors using cells expressing genetic constructs of a fusion protein of at least a binding domain of an auxiliary protein and a fragment of β-galactosidase, a fusion protein of an endosome-associated protein and a complementary fragment of β-galactosidase, and a wild-type receptor. The receptors are characterized by binding to the auxiliary protein-binding domain upon activation by an agonist and then endocytosing associated with an endosome to which the endosome-associated protein binds. Cells are incubated with a candidate ligand followed by lysis with a lysing medium comprising a substrate for the β-galactosidase. The enzyme product is then detected as a measure of the activation of the receptor. | 08-12-2010 |
20120077204 | Detection of Protein Translocation by Beta-Galactosidase Reporter Fragment Complementation - Methods and compositions are provided for detecting molecular translocations, particularly protein translocations within and between sub-cellular compartments, using at least two components that exhibit a localization-dependent difference in complementation activity. In particular, alpha-complementing β-galactosidase fragments are provided. These β-galactosidase reporter fragments display significantly enhanced enzymatic activity when one fragment is localized in a membrane. Methods for carrying out no-wash ELISA assays based on the reporter component system are also provided. | 03-29-2012 |
20120329075 | MONITORING PROTEIN TRAFFICKING USING BETA-GALACTOSIDASE REPORTER FRAGMENT COMPLEMENTATON - Methods and materials are disclosed for use in an enzyme fragment complementation assay using complementary fragments of β-galactosidase to study the trafficking of proteins in a cell. Compounds that bind to a target peptide have been found to affect protein folding and therefore trafficking. β-Galactosidase fragments, an enzyme donor (ED) and an enzyme acceptor (EA), are fused to a target peptide and to an intracellular compartment protein, wherein the compartment is involved in intracellular trafficking. Contacting the cell with a compound that binds to the target peptide results in enhanced movement of the protein through the cellular trafficking pathway comprised of the endoplasmic reticulum, Golgi apparatus, the plasma membrane, endosomes, etc. Using this approach, compounds that bind to a target peptide and alter its ability to traffic through the normal cellular pathway can be readily detected. | 12-27-2012 |
20130203067 | DETECTION OF INTRACELLULAR BINDING EVENTS BY MEASURING PROTEIN ABUNDANCE - Methods and compositions are provided to measure the binding of a test compound to a target peptide by measuring the effect of the compound on the abundance of the target peptide inside a cell. The target peptide may bind the test compound at an active site or an allosteric site, and it has been found that such binding may stabilize the target peptide against cellular degradation. The target peptide will preferably comprise a destabilizing mutation which shortens the half life of the target peptide within the cell, typically a mammalian cell. Test compounds, including small molecules, have been found to stabilize target peptides. Also provided are systems and kits for use in practicing the methods. | 08-08-2013 |
Thomas Scott Wehrman, Mountain View, CA US
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20090098588 | BETA GALACTOSIDASE DONOR FRAGMENTS - Truncated fragments of the small fragment of β-galactosidase are provided that have low affinity for the large fragment of β-galactosidase and provide for robust signals when two fusion proteins are complexed due to the binding of the proteins to which the β-galactosidase fragments are fused. The truncated fragments do not interfere with the complexing of the two proteins and allow for the two proteins to function and be responsive to candidate compounds that affect complex formation. | 04-16-2009 |
Thomas Scott Wehrman, Sunnyvale, CA US
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20100203543 | BIOLOGICAL ENCODING OF LARGE NUMBERS OF CELLS - Mixtures of cell types can be analyzed by having at least two signal markers, with at least one at three different levels to provide a barcode for each cell type. The mixture of cells may be subjected to a common candidate moiety and the effect of the moiety on the cells determined along with identification of the cell by the barcode. Conveniently, surface marker proteins and labeled antibodies can be used to create the barcode and the cells analyzed with flow cytometry. | 08-12-2010 |
Tom Wehrman, Fremont, CA US
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20100285451 | DETECTION OF SUB-CELLULAR COMPARTMENT LOCALIZATION OF A MOLECULE USING A REDUCED AFFINITY ENZYME COMPLEMENTATION REPORTER SYSTEM - Methods and compositions for detecting the sub-cellular localization of a molecule are provided. Aspects of the invention include detecting translocation of a cell-surface receptor to a sub-cellular compartment, e.g., the endosome, using a reduced affinity enzyme complementation reporter system. Also provided are systems and kits for use in practicing embodiments of the methods. | 11-11-2010 |
20140178913 | Detection of Molecular Interactions Using a Reduced Affinity Enzyme Complementation Reporter System - Methods and compositions for detecting molecular interactions are provided. Aspects of the invention include the use of a reduced affinity enzyme complementation reporter system. Also provided are systems and kits for use in practicing embodiments of the methods. | 06-26-2014 |