Patent application number | Description | Published |
20100093029 | MODIFIED LUCIOLA CRUCIATA LUCIFERASE GENE AND PROTEIN - A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from | 04-15-2010 |
20100233744 | Enzyme substrates for visualizing acidic organelles - The present invention relates to the visualization of acidic organelles based upon organelle enzyme activity. The organelle substrates of the invention are specific for enzyme activity of the organelle and label these organelles, such as lysosomes, rendering them visible and easily observed. Substrates of the present invention include substrates that produce a fluorescent signal. The fluorogenic acidic organelle enzyme substrates of this invention are designed to provide high fluorescence at low pH values and are derivatized to permit membrane permeation through both outer and organelle membranes of intact cells and can be used for staining cells at very low concentrations. They can be used for monitoring enzyme activity in cells at very low concentrations and are not toxic to living cells or tissues. | 09-16-2010 |
20120028257 | Modified luciola cruciata luciferase gene and protein - A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from | 02-02-2012 |
20120034634 | MODIFIED LUCIOLA CRUCIATA LUCIFERASE PROTEIN - A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from | 02-09-2012 |
20130252268 | Enzyme substrates for visualizing acidic organelles - The present invention relates to the visualization of acidic organelles based upon organelle enzyme activity. The organelle substrates of the invention are specific for enzyme activity of the organelle and label these organelles, such as lysosomes, rendering them visible and easily observed. Substrates of the present invention include substrates that produce a fluorescent signal. The fluorogenic acidic organelle enzyme substrates of this invention are designed to provide high fluorescence at low pH values and are derivatized to permit membrane permeation through both outer and organelle membranes of intact cells and can be used for staining cells at very low concentrations. They can be used for monitoring enzyme activity in cells at very low concentrations and are not toxic to living cells or tissues. | 09-26-2013 |
20140087402 | Synthetic luciferase gene and protein - A codon optimized and stabilized luciferase gene and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase. Assays using this new enzyme for measuring various biological metabolic functions are described. | 03-27-2014 |