Tsunasawa
Shinya Tsunasawa, Kawasaki JP
Patent application number | Description | Published |
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20080208699 | SALES-ASSISTANCE PROCESSING METHOD, APPARATUS, AND COMPUTER-READABLE MEDIUM - A method, system, and computer-readable medium of sales assistance include transmitting between a first sales site and a server a first message indicating that a product is unavailable at the first sales site. The method, system, and computer-readable medium also include determining and transmitting, between the server and the first sales site, a second message indicating a location of a second sales site at which the product is available, and displaying the location at the first sales site. | 08-28-2008 |
Susumu Tsunasawa, Kyoto JP
Patent application number | Description | Published |
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20090325227 | C-TERMINUS MODIFICATION METHOD, C-TERMINUS IMMOBILIZATION METHOD AND ANALYSIS METHOD FOR PROTEIN OR PEPTIDE - The present invention provides a method for inexpensively, easily and efficiently modifying the C-terminus of a protein or peptide; a method for easily and reliably isolating a C-terminal peptide fragment of a protein or peptide; and a method for rapidly, accurately and reliably determining an amino acid sequence of a protein or peptide by using a mass spectroscope. A method comprising the step of adding a formylation reagent and a catalyst to a protein or peptide to convert a carboxyl group into an aldehyde group. A method comprising the step of reacting a nucleophilic reagent with the aldehyde group to modify the C-terminus of the protein or peptide. A method comprising the step of reacting a support having a nucleophilic group to immobilize the protein or peptide. A method comprising the step of fragmenting the immobilized protein or peptide, washing the support, and isolating the C-terminal peptide fragment from the support. A method comprising the step of subjecting the isolated C-terminal peptide fragment to mass spectrometry and determining an amino acid sequence. | 12-31-2009 |
Susumu Tsunasawa, Otsu-Shi JP
Patent application number | Description | Published |
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20090029416 | HYPERTHERMOSTABLE PROTEASE GENE - There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID Nos. 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene. | 01-29-2009 |
20090142851 | METHOD FOR SELECTIVELY RECOVERING C-TERMINAL PEPTIDE OF PROTEIN AND METHOD FOR DETERMINING AMINO ACID SEQUENCE OF C-TERMINAL PEPTIDE OF PROTEIN USING THE SAME - The present invention provides a method for specifically recovering a C-terminal peptide fragment, and a method for easily determining the sequence of a C-terminal peptide fragment, which is difficult to be determined by a conventional method, with the use of a mass spectrometer, in particular a method capable of de novo sequencing of a C-terminal peptide fragment. A method for selectively recovering a C-terminal peptide of a protein, comprising the steps of: in a cleavage product of a protein containing a C-terminal peptide fragment (A) having an α-amino group but not having an ε-amino group and the other peptide fragments (B) having an α-amino group and an ε-amino group, selectively modifying the α-amino groups to obtain a C-terminal peptide fragment modified (A′) and the other peptide fragments modified (B′); and separating the C-terminal peptide fragment modified (A′) from the modified cleavage product by allowing a carrier to hold the other peptide fragments modified (B′) via the ε-amino group. A method for determining the amino acid sequence of a C-terminal peptide of a protein, comprising the steps of: selectively recovering a C-terminal peptide of a protein by the above method; and determining the amino acid sequence by subjecting a recovered C-terminal peptide fragment to mass spectrometry measurement. | 06-04-2009 |