Patent application number | Description | Published |
20100209392 | Excipients for use in adeno-associated virus pharmaceutical formulations, and pharmaceutical formulations made therewith - Stable pharmaceutical compositions comprising recombinant adeno-associated virus (AAV) virions are described. The compositions provide protection against loss of recombinant AAV vector genomes and transduceability under conditions such as exposure to cycles of freezing and thawing and storage in glass or polypropylene vials. The compositions comprise recombinant AAV virions in combination with one or more dihydric or polyhydric alcohols, and, optionally, a detergent, such as a sorbitan ester. Also described are methods of using the compositions. | 08-19-2010 |
20120219528 | Excipients for use in adeno-associated virus pharmaceutical formulations, and pharmaceutical formulations made therewith - Stable pharmaceutical compositions comprising recombinant adeno-associated virus (AAV) virions are described. The compositions provide protection against loss of recombinant AAV vector genomes and transduceability under conditions such as exposure to cycles of freezing and thawing and storage in glass or polypropylene vials. The compositions comprise recombinant AAV virions in combination with one or more dihydric or polyhydric alcohols, and, optionally, a detergent, such as a sorbitan ester. Also described are methods of using the compositions. | 08-30-2012 |
20150238610 | EXCIPIENTS FOR USE IN ADENO-ASSOCIATED VIRUS PHARMACEUTICAL FORMULATIONS, AND PHARMACEUTICAL FORMULATIONS MADE THEREWITH - Stable pharmaceutical compositions comprising recombinant adeno-associated virus (AAV) virions are described. The compositions provide protection against loss of recombinant AAV vector genomes and transduceability under conditions such as exposure to cycles of freezing and thawing and storage in glass or polypropylene vials. The compositions comprise recombinant AAV virions in combination with one or more dihydric or polyhydric alcohols, and, optionally, a detergent, such as a sorbitan ester. Also described are methods of using the compositions. | 08-27-2015 |
Patent application number | Description | Published |
20110206610 | COMPOSITIONS AND METHODS FOR ENHANCING DRUG DELIVERY ACROSS AND INTO EPITHELIAL TISSUES - This invention provides compositions and methods for enhancing delivery of drugs and other agents across epithelial tissues, including the skin, gastrointestinal tract, pulmonary epithelium, ocular tissues and the like. The compositions and methods are also useful for delivery across endothelial tissues, including the blood brain barrier. The compositions and methods employ a delivery enhancing transporter that has sufficient guanidino or amidino sidechain moieties to enhance delivery of a compound conjugated to the reagent across one or more layers of the tissue, compared to the non-conjugated compound. The delivery-enhancing polymers include, for example, poly-arginine molecules that are preferably between about 6 and 25 residues in length. | 08-25-2011 |
20130096069 | COMPOSITIONS AND METHODS FOR ENHANCING DRUG DELIVERY ACROSS AND INTO OCULAR TISSUES - This invention provides compositions and methods for enhancing delivery of drugs and other agents across epithelial tissues, including into and across ocular tissues and the like. The compositions and methods are also useful for delivery across endothelial tissues, including the blood brain barrier. The compositions and methods employ a delivery enhancing transporter that has sufficient guanidino or amidino sidechain moieties to enhance delivery of a compound conjugated to the reagent across one or more layers of the tissue, compared to the non-conjugated compound. The delivery-enhancing polymers include, for example, poly-arginine molecules that are preferably between about 6 and 25 residues in length. | 04-18-2013 |
20140213532 | COMPOSITIONS AND METHODS FOR ENHANCING DRUG DELIVERY ACROSS AND INTO EPITHELIAL TISSUES - This invention provides compositions and methods for enhancing delivery of drugs and other agents across epithelial tissues, including the skin, gastrointestinal tract, pulmonary epithelium, ocular tissues and the like. The compositions and methods are also useful for delivery across endothelial tissues, including the blood brain barrier. The compositions and methods employ a delivery enhancing transporter that has sufficient guanidine or amidino sidechain moieties to enhance delivery of a compound conjugated to the reagent across one or more layers of the tissue, compared to the non-conjugated compound. The delivery-enhancing polymers include, for example, poly-arginine molecules that are preferably between about 6 and 25 residues in length. | 07-31-2014 |
Patent application number | Description | Published |
20140174926 | MOLECULAR DIAGNOSTICS PLATFORM - A method of mixing a droplet, the method comprising providing a droplet on a surface, forming the droplet into a first “U” shape having a bottom region and two terminal ends, and simultaneously merging the terminal ends and splitting the droplet at the bottom region to form a second “U” shape which is substantially opposite the first “U” shape. | 06-26-2014 |
20150107995 | Droplet Actuator Devices and Methods for Manipulating Beads - The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads. | 04-23-2015 |
20150184219 | Enzyme Assays on a Droplet Actuator - The invention is directed to droplet actuator devices and assay methods. The invention includes assay methods of conducting an assay comprising combining a sample with an umbelliferyl derivative, wherein the sample potentially comprises an enzyme capable of cleaving the umbelliferyl derivative and where the umbelliferyl derivative comprises an umbelliferyl core modified with one or more modifying moieties. | 07-02-2015 |
Patent application number | Description | Published |
20110203930 | Bead Incubation and Washing on a Droplet Actuator - The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay. | 08-25-2011 |
20150238975 | BEAD MANIPULATION TECHNIQUES - The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads. | 08-27-2015 |
20150268235 | Bead Incubation and Washing on a Droplet Actuator - The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay. | 09-24-2015 |
20150285792 | Manipulation of Beads in Droplets and Methods for Manipulating Droplets - The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered. | 10-08-2015 |
20150314293 | Droplet Actuator Devices and Methods Employing Magnetic Beads - A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged for conducting droplet operations on a surface; (ii) a starting droplet comprising: (1) one or more magnetically responsive beads; (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone; (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet substantially lacking the magnetically responsive beads. | 11-05-2015 |
20160051992 | Bead Manipulation Techniques - The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads. | 02-25-2016 |
Patent application number | Description | Published |
20140302305 | HYDROPHOBIC POROUS HARD COATING WITH LUBRICANT, METHOD FOR MAKING AND USE OF SAME - A composite includes a porous matrix that includes a molybdenum-silicon-boron (Mo—Si—B) alloy that has a plurality of pores with a lubricant in contact with the Mo—Si—B alloy, a hydrophobic compound in contact with the Mo—Si—B alloy, or a combination thereof. A method for preparing a porous composite includes disposing a porous matrix comprising a Mo—Si—B alloy on a substrate, the Mo—Si—B alloy comprising a plurality of pores; disposing a lubricant on a surface of the porous matrix; and disposing a hydrophobic compound on a surface of the porous matrix to form the porous composite. | 10-09-2014 |
20150060051 | METHODS OF FORMING BORIDED DOWNHOLE TOOLS, AND RELATED DOWNHOLE TOOLS - A method of forming a downhole tool comprises contacting at least one downhole structure comprising at least one metal material with a molten electrolyte comprising anhydrous sodium tetraborate. Electrical current is applied to at least a portion of the at least one downhole structure to form at least one borided downhole structure comprising at least one metal boride material. Other methods of forming a downhole tool, and a downhole tool are also described. | 03-05-2015 |
20150060288 | METHODS OF FORMING BORIDED DOWN HOLE TOOLS, AND RELATED DOWN-HOLE TOOLS - A method of forming a down-hole tool comprises contacting at least a portion of at least one down-hole structure comprising at least one ceramic-metal composite material with a molten electrolyte comprising sodium tetraborate. Electrical current is applied to at least a portion of the at least one down-hole structure to form at least one borided down-hole structure comprising at least one metal boride material. Other methods of forming a down-hole tool, and a down-hole tool are also described. | 03-05-2015 |
20150240146 | DOWNHOLE TOOLS HAVING HYDROPHOBIC WEAR AND EROSION RESISTANT COATINGS, AND METHODS OF MANUFACTURING SUCH TOOLS - Downhole tools for use in wellbores include a layer of material over a body, wherein the layer is relatively more hydrophobic at higher temperatures and pressures, such as those encountered downhole within the wellbore, compared to the hydrophobicity at ambient conditions. For example, a downhole tool may include a body having a first composition, and a layer of material disposed at the surface of the body. The layer of material may comprise a boride or a nitride. An exposed surface of the layer of material exhibits a first relatively higher hydrophobicity at a temperature of 150° C. and a pressure of 1,300 psi, and exhibits a second relatively lower hydrophobicity at 20° C. and 14.70 psi. Methods of forming downhole tools include forming such a layer of material at a surface of a body of a downhole tool. | 08-27-2015 |
20160090824 | DOWNHOLE TOOLS HAVING HYDROPHOBIC COATINGS, AND METHODS OF MANUFACTURING SUCH TOOLS - A downhole tool for use in wellbores comprises a layer of hydrophobic material over a body, wherein the layer of hydrophobic material comprises a transition metal boride having a higher hydrophobicity than the body. The downhole tool may comprise a body having a composition and the layer of hydrophobic material comprising a discontinuous phase of the transition metal binder dispersed within a first continuous phase comprising a metal binder. The layer of material may be chemically bonded to the body. An interface between the body and the layer of material may comprise the transition metal boride dispersed within a second continuous phase comprising the metal binder and the composition of the body. Methods of forming downhole tools include forming such a layer of material at a surface of a body of a downhole tool. | 03-31-2016 |
20160097244 | Method for Increasing the Roughness of Injector Gripper Blocks for Coiled Tubing Operations - A method of increasing a roughness of coiled tubing injector blocks that includes providing a pair of gripper blocks each having a gripper surface configured to grip coiled tubing within an injector head and increasing a first roughness on the gripping surfaces to a second roughness. A coating may be applied to the gripping surfaces to increase the roughness. The coating may be chromium carbide, molybdenum boride, iron boride, titanium boride, or a transitional metal boride. The gripping surfaces may be treated to increase the roughness to a second roughness. The gripping surfaces may be blasted by abrasives or shot peened to increase the roughness. The second roughness may be greater than 20 μm. A system to inject coiled tubing into a wellbore may include an injector head, coiled tubing, and at least two gripper blocks having a gripping surface with a roughness of at least 20 μm. | 04-07-2016 |