Hart, OR
Courtenay Hart, Eugene, OR US
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20090081722 | SITE-SPECIFIC LABELING OF AFFINITY TAGS IN FUSION PROTEINS - The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment. | 03-26-2009 |
20120270757 | SITE-SPECIFIC LABELING OF AFFINITY TAGS IN FUSION PROTEINS - The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. | 10-25-2012 |
20140038856 | Site-specific labeling of affinity tags in fusion proteins - The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment. | 02-06-2014 |
Courtenay R. Hart, Eugene, OR US
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20100267078 | Selective Detection of Proteins that Contain Two or More Alpha-Helical Transmembrane Domains - Embodiments of the present invention provide a staining solution and of method of using the staining solution for selectively detecting proteins that contain two or more α-helical transmembrane domains. The staining solution comprises a lipophilic dyes and at least about a 30% hydrophobic solvent. The dyes of the present are represented by the general formula A-B-E wherein A is a nitrogen heterocycle, B is a bridge moiety and E is an electron pair accepting moiety that comprises either a carbonyl or nitrogen atom. In one embodiment these lipophilic dyes are merocyanine dye, a cyanine dye, a styryl dye or a carbazolylvinyl dye. | 10-21-2010 |
Courtenay Rae Hart, Eugene, OR US
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20090004641 | SITE-SPECIFIC LABELING OF AFFINITY PEPTIDES IN FUSION PROTEINS - The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment. | 01-01-2009 |
David Hart, Bend, OR US
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20150328350 | Apparatus and Method for Sanitizing - An apparatus and system that efficiently and effectively delivers a sanitizing fluid to ducts in the form of a fog or mist is described. A canister that contains a liquid sanitizing solution is attached to a compressor and includes a straw that has an internal passageway that extends into the liquid and a venturi tube above the level of the liquid that connects with the internal passageway in the straw. The headspace above the liquid in the canister is pressurized, causing fluid to flow upwardly in the straw when a trigger is depressed, drawing fluid upwardly in part by the venturi. As the liquid passes the venturi the pressurized air that is flowing into the venturi causes the fluid to form a fine mist. The mist, under pressure from the compressor, is routed into a flexible delivery tube that has been previously positioned in a duct system. The terminal end of the tube has an atomizing ball attached thereto and the atomizing ball has a series of orifices through which the misted, atomized fluid is delivered. As fogged sanitizing solution flows through the orifices it is deposited on the interior surfaces of the duct. The delivery tube and the attached atomizing ball are withdrawn from the duct as the sanitizing fog is emitted from the ball. The fan in the HVAC system is not activated so there is no deliver of the sanitizer through registers and into the living space. The apparatus and method may be used to apply other fluids to other surfaces. | 11-19-2015 |
David Hart, Cornelius, OR US
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20090302973 | Bulk Acoustic Resonators with Multi-Layer Electrodes - Bulk acoustic resonators with multi-layer electrodes for Bulk Acoustic Wave (BAW) resonator devices. Various electrode combinations are disclosed. The invention provides a better compromise at resonant frequencies from 1800 MHz to 4 GHz in terms of keff2 and resistance than state of the art solutions using either Mo, or a bilayer of Al and W. | 12-10-2009 |
20100005654 | Planarization Methods - Planarization methods for maintaining planar surfaces in the fabrication of such devices as BAW devices and capacitors on a planar or planarized substrate are described. In accordance with the method, a metal layer is deposited and patterned, and an oxide layer is deposited using a high density plasma chemical vapor deposition (HDP CVD) process to a thickness equal to the thickness of the metal layer. The HDP CVD process provides an oxide layer on the patterned metal tapering upward from the edge of the patterned metal layer. Then, after masking and etching the oxide layer from the patterned metal layer, the patterned metal layer and surrounding oxide layer form a substantially planar layer, interrupted by small remaining oxide protrusions at the edges of the patterned layer. These small remaining oxide protrusions may be too small to significantly disturb the flatness of a further oxide or other layer or they may be further mitigated by the application of another HDP CVD oxide film. | 01-14-2010 |
David E. Hart, Cornelius, OR US
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20130082320 | STRAPPED DUAL-GATE VDMOS DEVICE - Semiconductor devices are described that include a dual-gate configuration. In one or more implementations, the semiconductor devices include a substrate having a first surface and a second surface. The substrate includes a first and a second body region formed proximal to the first surface. Moreover, each body region includes a source region formed therein. The substrate further includes a drain region formed proximal to the second surface and an epitaxial region that is configured to function as a drift region between the drain region and the source regions. A dual-gate is formed over the first surface of the substrate. The dual-gate includes a first gate region and a second gate region that define a gap there between to reduce the gate to drain capacitance. A conductive layer may be formed over the first gate region and the second gate region to lower the effective resistance of the dual-gate. | 04-04-2013 |
Joel Edward Hart, Tualatin, OR US
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20130135111 | SELF-CALIBRATING CURRENT SWITCH WITH DISPLAY - A current switch self-calibrates when a reset control is actuated and displays an expected current in the monitored conductor, one or more trip points of the current switch and the last known current in the monitored conductor. | 05-30-2013 |
Kenneth M. Hart, Klamath Falls, OR US
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20090165406 | INTEGRAL SILL CORNER WITH UPSTAND - A sill corner for use in a sill assembly supported on a base surface, the sill assembly comprising a vertical jamb and a lineal base member. The sill corner comprises a vertical component with a top surface adapted to receive the vertical jamb and a bottom surface adapted to abut the base surface. A horizontal component extends transversely from the vertical component and the horizontal and the vertical components are a unitary element. The top and bottom surfaces of the vertical component are separated by a height, the height being between about 1.00 and about 3.00 inches. | 07-02-2009 |
20100162644 | SILL CORNER WITH PATHWAY - A sill corner key for use in a sill assembly configured to be supported on a base surface, the sill assembly including a lineal base member and a vertical jamb. The sill corner key includes a vertical component having a top surface, a bottom surface, and an inner wall extending between the top surface and the bottom surface, the inner wall defining a sealant pathway formed therein. The inner wall is configured to slidably receive a portion of the lineal base member and the sealant pathway is configured to direct a fluid sealant to flow along the portion of the lineal base member within the inner wall. | 07-01-2010 |
Richard L. Hart, Portland, OR US
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20080308325 | Powered trailer dolly - A towing dolly is provided for relocating wheeled loads such as boat trailers, camping trailers and airplanes where towing forces required may be substantial. The towing dolly may have a wheel assembly with a drive gear disposed between proximate treads. The towing dolly may have a narrow wheel width providing easy steering and compact size for storage. The towing dolly may have a motor with a gear sprocket. A linked chain may transfer power from the motor to the drive gear located between the double treads. The dolly may have a steering arm extending perpendicular to drive direction allowing the operator to stand away from path of movement of the load while providing adequate leverage to steer the dolly. The dolly may have an alternate configuration with a power source located on the dolly providing operation independent of plugins or extension cords. | 12-18-2008 |
Robert Hart, Portland, OR US
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20110098710 | Preparatory Reamers For Orthopedic Implants - A reamer for preparing a spinal upper facet to facilitate insertion of combined screw/washer implant, preventing bone damage and facet weakening, and also allowing possibility to place grafting so as to maximize implant stabilization. The reamer has shallow outer cutting surfaces to cut a groove for spike insertion; deep inner cutting surfaces to cut a cortical-penetrating bore for insertion of the screw, and a flat therebetween to limit penetration of these cutting surfaces. | 04-28-2011 |
Robert A. Hart, Portland, OR US
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20120010662 | PEDICULAR FACET FUSION SCREW WITH PLATE - Spinal implants and methods for spinal stabilization and/or fusion are provided. Exemplary implants described herein can be configured for delivery to a facet joint to stabilize and/or fuse the facet joint, and can optionally be anchored within the pedicle for added fixation. The implant can optionally include a fusion-promoting bioactive material thereby providing a single device capable of spinal stabilization and/or fusion. Furthermore, a method of placing such an implant within a facet joint is provided. | 01-12-2012 |
Robert Alan Hart, Portland, OR US
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20100268230 | METHOD AND APPARATUS FOR DENS FRACTURE FIXATION - Embodiments of the present invention provide methods, apparatuses, and systems for fixing dens fractures. The mode of failure for screw fixation of C2 dens fractures is often via cut-out at the anterior body. In an embodiment, securing a plate, such as a locking plate, to the anterior surface of the vertebra attached directly to an interfragmentary screw may reduce potential for anterior screw cut-out and improve construct strength. Plate supplementation of anterior screw fixation of Type II dens fractures thus improves construct strength and changes the failure mechanism from anterior screw cut-out to posterior displacement of the screw, thus improving clinical outcomes for these fractures. | 10-21-2010 |
Robert M. Hart, Portland, OR US
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20120010669 | PEDICULAR FACET FUSION SCREW WITH PLATE - Spinal implants and methods for spinal stabilization and/or fusion are provided. Exemplary implants described herein can be configured for delivery to a facet joint to stabilize and/or fuse the facet joint, and can optionally be anchored within the pedicle for added fixation. The implant can optionally include a fusion-promoting bioactive material thereby providing a single device capable of spinal stabilization and/or fusion. Furthermore, a method of placing such an implant within a facet joint is provided. | 01-12-2012 |