Patent application number | Description | Published |
20090275087 | ASYMMETRIC ADAPTER LIBRARY CONSTRUCTION - The present invention provides methods and compositions for asymmetrically tagging a nucleic acid fragment using asymmetric adapters. | 11-05-2009 |
20100015666 | ISOTHERMAL DNA AMPLIFICATION - The present invention provides for amplification of one or more polynucleotides by multi-staged linear amplifications using one or more RNA polymerases. At each stage RNA transcripts are accumulated at a linear rate, so that multiple stages provide for faster than linear transcript accumulation. In one aspect, the invention provides for polynucleotide amplification by ligating hairpin adaptors to an end of polynucleotides wherein the hairpin adaptors each contain a promoter sequence oriented so that transcription proceeds in the direction of the loop of the hairpin adaptor. Upon transcription through such loop region and to the complementary strand a replicate is made of the promoter sequence as well as the polynucleotide, thereby permitting exponential amplification upon reverse transcription, second strand synthesis, and repetition of the above cycle. Preferably such amplification is carried out under isothermal reaction conditions. | 01-21-2010 |
20120083017 | ASYMMETRIC ADAPTER LIBRARY CONSTRUCTION - The present invention provides methods and compositions for asymmetrically tagging a nucleic acid fragment using asymmetric adapters. | 04-05-2012 |
20120122161 | Sorting Asymmetrically Tagged Nucleic Acids by Selective Primer Extension - The present invention provides methods and compositions for amplifying and sorting adapter tagged nucleic acid fragments using selective primer extension. Immortalized pooled polynucleotide samples and method of producing the same are also provided. | 05-17-2012 |
20120135407 | Compositions and Methods for Intramolecular Nucleic Acid Rearrangement - Aspects of the present invention are drawn to processes for moving a region of interest in a polynucleotide from a first position to a second position with regard to a domain within the polynucleotide, also referred to as a “reflex method”. In certain embodiments, the reflex method results in moving a region of interest into functional proximity to specific domain elements present in the polynucleotide (e.g., primer sites and/or MID). Compositions, kits and systems that find use in carrying out the reflex processes described herein are also provided. | 05-31-2012 |
20120245041 | BASE-BY-BASE MUTATION SCREENING - Aspects of the present invention are drawn to screening assays for isolating polynucleotides having a sequence variation or mutation. Embodiments of the screening assays include generating a population of polynucleotide duplexes having 5′ overhang regions on one strand of the duplex (the “template” or “bottom strand”) followed by isolating polynucleotide duplexes from the mixture that have one or more mismatched base at the 3′ end of the other strand of the duplex (the “test” or “top” strand). | 09-27-2012 |
20130029380 | Asymmetric Adapter Library Construction - The present invention provides methods and compositions for asymmetrically tagging a nucleic acid fragment using asymmetric adapters. | 01-31-2013 |
20130059310 | Compositions and Methods for Intramolecular Nucleic Acid Rearrangement - Aspects of the present invention are drawn to processes for moving a region of interest in a polynucleotide from a first position to a second position with regard to a domain within the polynucleotide, also referred to as a “reflex method”. In certain embodiments, the reflex method results in moving a region of interest into functional proximity to specific domain elements present in the polynucleotide (e.g., primer sites and/or MID). Compositions, kits and systems that find use in carrying out the reflex processes described herein are also provided. | 03-07-2013 |
20130220812 | Compositions and Methods for Intramolecular Nucleic Acid Rearrangement - Aspects of the present invention are drawn to processes for moving a region of interest in a polynucleotide from a first position to a second position with regard to a domain within the polynucleotide, also referred to as a “reflex method”. In certain embodiments, the reflex method results in moving a region of interest into functional proximity to specific domain elements present in the polynucleotide (e.g., primer sites and/or MID). Compositions, kits and systems that find use in carrying out the reflex processes described herein are also provided. | 08-29-2013 |
20140030704 | Asymmetric Adapter Library Construction - The present invention provides methods and compositions for asymmetrically tagging a nucleic acid fragment using asymmetric adapters. | 01-30-2014 |