Patent application number | Description | Published |
20090093034 | Method for Separation of Lactic Acid Component from Lactic Acid Fermentation Liquor, and Separation Apparatus - A lactic acid component (e.g., lactic acid or oligo (lactic acid)) can be obtained by extraction from a lactic acid fermentation liquor with a pH of 4.8 or less, using at least one solvent selected from the group consisting of toluene, xylene, mesitylene, ethylbenzene, methanol, ethanol, propanol, butanol, and mineral spirit. Furthermore, oligo (lactic acid) can be obtained, by heating a lactic acid fermentation liquor with a pH of 4.8 or less under reduced pressure, and washing, with water, the fermentation liquor containing a produced oligo (lactic acid). Hence, a method is provided for separating a lactic acid component from a lactic acid fermentation liquor, which is free from incorporation of impurities and which includes simple steps. | 04-09-2009 |
20100261235 | CONTINUOUS PRODUCTION OF BIODIESEL FUEL BY ENZYMATIC METHOD - A method for continuously producing a fatty acid ester of the present invention comprises (a) mixing and agitating an oil and fat starting material and a lower alcohol, and supplying a mixture to one of the catalyst reaction tubes filled with a lipase; (b) producing a fatty acid ester and glycerin in the catalyst reaction tube; (c) introducing an outflowing liquid from the catalyst reaction tube into a glycerin separation tank, thereby collecting the glycerin; (d) adding a lower alcohol to a separated liquid obtained by separating the glycerin from the outflowing liquid, mixing and agitating an obtained material, and supplying a mixture to a following catalyst reaction tube; (e) repeating the steps (b) to (d) until supply to a last catalyst reaction tube is performed; and (f) collecting a fatty acid ester from the separated liquid obtained from the last catalyst reaction tube. According to the method of the present invention, the concentration of a lower alcohol can be strictly controlled and by product glycerin can be automatically removed. | 10-14-2010 |
20110081698 | METHOD AND INTRODUCTION OF GENE INTO YEAST CELL, AND VECTOR FOR THE METHOD - An object of the present invention is a method for introducing a foreign gene into a yeast cell that does not have an auxotrophic marker. The present invention provides a method for providing a target auxotrophy to a yeast cell and introducing a gene to be expressed into the yeast cell. The method includes the step of transforming a yeast cell with a fragment containing an expression cassette for the gene to be expressed, a cassette for a yeast selectable marker, and two homologous recombination fragments each homologous to a region on either side of a target auxotrophy controlling gene. According to the method, a target auxotrophy controlling gene is deleted from a yeast cell and a gene to be expressed is introduced into the yeast cell, and further the yeast selectable marker is eliminated from the transformed yeast cell. | 04-07-2011 |
20110183396 | PRODUCTION AND USE OF YEAST HAVING INCREASED CELLULOSE HYDROLYSIS ABILITY - The present invention provides a method for producing an yeast having an increased cellulose hydrolysis ability. The method includes the step of introducing increased integration copy numbers of both a gene for an enzyme capable of hydrolyzing crystalline cellulose and a gene for an enzyme capable of hydrolyzing noncrystalline cellulose into a noncellulolytic yeast to give a transformed yeast. The yeast having an increased cellulose hydrolysis ability can be suitably used for ethanol production from cellulose-based materials. | 07-28-2011 |
20110196136 | CONCENTRATED ACID TREATMENT UNIT, CONCENTRATED ACID TREATMENT METHOD, PHASE-SEPARATION SYSTEM PLANT FOR BOTANICAL RESOURCE, AND CONVERSION METHOD - Provided is a Phase-Separation system plant for botanical resource, a conversion method, a concentrated acid treatment unit, and a concentrated acid treatment method by which lignin derivatives and hydrolyzed carbohydrate are manufactured out of a botanical resource efficiently and continuously. A concentrated acid treatment unit ( | 08-11-2011 |
20120058528 | Homolactic Fermentation from Pentose - Provided is a lactic acid bacterium capable of homolactic fermentation using a pentose as a substrate, the lactic acid bacterium utilizing a pentose, and in which a phosphoketolase pathway is blocked and a pentose phosphate pathway is activated. Also provided is a method for producing lactic acid from a pentose using the lactic acid bacterium and a method for preparing the lactic acid bacterium. | 03-08-2012 |
20150037858 | METHOD FOR PRODUCING ETHANOL - A method for producing ethanol from lignocellulosic biomass using yeast at low cost is provided. The method of the present invention for producing ethanol from lignocellulosic biomass includes steps of (1) pretreating lignocellulosic biomass, (2) treating a cellulose fraction obtained in Step (1) with a cellulose hydrolase, (3) mixing saccharified biomass obtained in Step (2) with yeasts to perform ethanol fermentation, and (4) subjecting a fermentation product obtained in Step (3) to a solid-liquid separation, wherein a cycle consisting of Steps (1), (2), (3) and (4) is repeated twice or more, and yeasts obtained in Step (4) are used as all or a portion of yeasts in Step (3) of the subsequent cycle. | 02-05-2015 |
Patent application number | Description | Published |
20090275110 | Microorganism that Displays Biotin on Cell Surface - Provided is a microorganism that can display, on the cell surface, any molecules other than a molecule comprising amino acids, more specifically, a microorganism that displays biotin on a cell surface. The microorganism is capable of co-expressing a biotinylating enzyme and an acceptor peptide having a sequence recognized by the biotinylating enzyme, wherein the acceptor peptide is expressed on the cell surface, so that lysine of the acceptor peptide is biotinylated to display biotin on the cell surface. Also provided is a method for displaying an intended molecule, including not only a molecule comprising amino acids but also any molecules, on a cell surface of a microorganism. | 11-05-2009 |
20100016628 | Polyester Polyol - A star-branched polyester polyol is obtained by polymerizing lactide or lactic acid, using, as an initiator, a fat and oil composed mainly of a triacylglycerol that has at least three hydroxyl groups or epoxy groups in its molecule. This polyester polyol has low crystallinity and a low melting point, and thus shows good working properties when used in various applications. Furthermore, this polyester polyol is derived from renewable resources, and, thus, it is highly desirable in view of its effectiveness in protecting the global environment and preventing fossil resources from being depleted. | 01-21-2010 |
20120149116 | CELLULOSE DEGRADABLE YEAST AND METHOD FOR PRODUCTION THEREOF - The present invention provides a method for producing a cellulose degradable yeast, comprising the step of co-introducing genes coding for at least two cellulose-degrading enzymes into a yeast host via integration with a yeast δ sequence. According to the invention, a yeast having an improved cellulose degradation ability are provided. | 06-14-2012 |
20120282666 | METHOD FOR PRODUCING ETHANOL - The present invention provides a method for producing ethanol, including: subjecting a cellulose-based material to a hot-water treatment and an ammonia treatment to obtain a fermentation substrate; and reacting the fermentation substrate with a yeast to produce ethanol. The present invention also provides a method for producing a fermentation substrate for ethanol production by a yeast, including: subjecting a cellulose material to a hot-water treatment and an ammonia treatment to obtain a fermentation substrate. Thus obtained fermentation substrate can enhance the ethanol production by yeast. | 11-08-2012 |