Patent application number | Description | Published |
20120107268 | LYMPH NODE-TARGETING NANOPARTICLES - Provided are nanoparticles comprising heparin, chitosan, and at least one immunomodulatory agent, e.g. a cytokine. The cytokine can be selected from the group consisting of TNF, IL-12, IL-2, IL-23, IL-1α, IL-10, IL-18, and combinations thereof. Further provided are methods of making a nanoparticle comprising mixing a first composition comprising heparin with a second composition comprising chitosan in the presence of at least one cytokine to form a third composition. Further provided are methods of modulating an immune response comprising co-administering to a subject an antigen or vaccine with nanoparticles comprising heparin, chitosan, and at least one cytokine. | 05-03-2012 |
20150023909 | LYMPH NODE-TARGETING NANOPARTICLES - Provided are nanoparticles comprising heparin, chitosan, and at least one immunomodulatory agent, e.g. a cytokine. The cytokine can be selected from the group consisting of TNF, IL-12, IL-2, IL-23, IL-1α, IL-10, IL-18, and combinations thereof. Further provided are methods of making a nanoparticle comprising mixing a first composition comprising heparin with a second composition comprising chitosan in the presence of at least one cytokine to form a third composition. Further provided are methods of modulating an immune response comprising co-administering to a subject an antigen or vaccine with nanoparticles comprising heparin, chitosan, and at least one cytokine. | 01-22-2015 |
Patent application number | Description | Published |
20100003687 | System and method for detection of HIV tropism variants - An embodiment of a method for detecting low frequency occurrence of one or more HIV sequence variants associated with drug resistance is described that comprises the steps of: generating cDNA species from each RNA molecule in an HIV sample population; amplifying at least one first amplicon from the cDNA species, wherein each first amplicon comprises a plurality of amplified copies and is amplified with a pair of nucleic acid primers that define a locus of the first amplicon; clonally amplifying the amplified copies of the first amplicons to produce a plurality of second amplicons wherein a plurality of the second amplicons comprise an immobilized population of substantially identical copies from one of the amplified copies of first amplicons; determining a nucleic acid sequence composition of the substantially identical copies from at least 100 of the immobilized populations in parallel on a single substrate; and detecting one or more sequence variants that occur at a frequency of 5% or less in the nucleic acid sequence composition of the at least 100 immobilized populations; and correlating the detected sequence variants with variation associated with HIV tropism. | 01-07-2010 |
20100136516 | System and method for detection of HIV integrase variants - An embodiment of a method for detecting low frequency occurrence of one or more HIV sequence variants associated with integrase is described that comprises the steps of: (a) generating a cDNA species from a plurality of RNA molecules in an HIV sample population; (b) amplifying a plurality of first amplicons from the cDNA species, wherein each first amplicon is amplified with a pair of nucleic acid primers; (c) clonally amplifying the amplified copies of the first amplicons to produce a plurality of second amplicons; (d) determining a nucleic acid sequence composition of the second amplicons; (e) detecting one or more sequence variants that occur at a frequency of 5% or less in the nucleic acid sequence composition of the second amplicons; and (f) correlating the detected sequence variants with variation associated with HIV integrase. | 06-03-2010 |
20110165556 | SYSTEM AND METHOD FOR DETECTION OF HIV TROPISM VARIANTS - An embodiment of a method for detecting low frequency occurrence of one or more HIV sequence variants associated with drug resistance is described that comprises the steps of: generating cDNA species from each RNA molecule in an HIV sample population; amplifying at least one first amplicon from the cDNA species, wherein each first amplicon comprises a plurality of amplified copies and is amplified with a pair of nucleic acid primers that define a locus of the first amplicon; clonally amplifying the amplified copies of the first amplicons to produce a plurality of second amplicons wherein a plurality of the second amplicons comprise an immobilized population of substantially identical copies from one of the amplified copies of first amplicons; determining a nucleic acid sequence composition of the substantially identical copies from at least 100 of the immobilized populations in parallel on a single substrate; and detecting one or more sequence variants that occur at a frequency of 5% or less in the nucleic acid sequence composition of the at least 100 immobilized populations; and correlating the detected sequence variants with variation associated with HIV tropism. | 07-07-2011 |
20120322665 | SYSTEM AND METHOD FOR DETECTION OF HIV-1 CLADES AND RECOMBINANTS OF THE REVERSE TRANSCRIPTASE AND PROTEASE REGIONS - A method for detecting low frequency occurrence of one or more HIV sequence variants associated with reverse transcriptase and/or protease is described that comprises the steps of: (a) generating a cDNA species from a plurality of RNA molecules in an HIV sample population; (b) amplifying a plurality of first amplicons from the cDNA species, wherein each first amplicon is amplified with a pair of nucleic acid primers capable of generating amplicons from an HIV clade comprising clade A, clade B, clade C, clade D, clade F, and clade G; (c) clonally amplifying the amplified copies of the first amplicons to produce a plurality of second amplicons; (d) determining a nucleic acid sequence composition of the second amplicons; and (e) detecting one or more sequence variants in the nucleic acid sequence composition of the second amplicons. | 12-20-2012 |
Patent application number | Description | Published |
20080280772 | Salivary Mrna Profiling, Biomarkers and Related Methods and Kits of Parts - A method to detect a biomarker in saliva wherein the biomarker is an extracellular mRNA, comprises detecting the extracellular mRNA in the cell-free saliva; transcriptome analysis of saliva comprises detecting a transcriptome pattern in the cell-free saliva; a method to detect genetic alterations in an organ or in a gene in the organ by analyzing saliva, comprises detecting a transcriptome pattern and/or the mRNA profiling of the gene in cell-free saliva; a method to diagnose an oral or systemic pathology disease or disorder in a subject, comprises: detecting profile of a biomarker associated with the pathology disease or disorder, in particular mRNA and/or protein, in cell-free saliva and/or serum; kits comprising identifier for at least one biomarker for performing at least one of the methods; and use of salivary biomarker salivary and/or serum mRNAs as biomarkers for oral and/or systemic pathology, disease or disorder. | 11-13-2008 |
20140249236 | Salivary mRNA Profiling, Biomarkers and Related Methods and Kits of Parts - A method to detect a biomarker in saliva wherein the biomarker is an extracellular mRNA, comprises detecting the extracellular mRNA in the cell-free saliva; transcriptome analysis of saliva comprises detecting a transcriptome pattern in the cell-free saliva; a method to detect genetic alterations in an organ or in a gene in the organ by analyzing saliva, comprises detecting a transcriptome pattern and/or the mRNA profiling of the gene in cell-free saliva; a method to diagnose an oral or systemic pathology disease or disorder in a subject, comprises: detecting profile of a biomarker associated with the pathology disease or disorder, in particular mRNA and/or protein, in cell-free saliva and/or serum; kits comprising identifier for at least one biomarker for performing at least one of the methods; and use of salivary biomarker salivary and/or serum mRNAs as biomarkers for oral and/or systemic pathology, disease or disorder. | 09-04-2014 |
20150094518 | MODULAR POLYMER PLATFORM FOR THE TREATMENT OF CANCER - The invention provides a novel polymer platform to deliver a desired combination of therapeutic agents to a site in need thereof for the treatment of cancer. In certain embodiments the platform is a modular polymer platform that allows for customization based upon the tumor of the subject to be treated. | 04-02-2015 |
Patent application number | Description | Published |
20140263055 | Ion Sequestration for Scale Prevention in High-Recovery Desalination Systems - Cations that can precipitate from an aqueous composition to produce scaling are sequestered by adding a multi-dentate ligand to the aqueous composition. The multi-dentate ligand bonds with the cation to form a non-scaling ionic complex; and the aqueous solution with the ionic complex is used in a process that produces substantially pure water from the aqueous composition, where the cation, absent formation of the ionic complex, is subject to scaling. The pH of the aqueous composition (or a brine including components of the aqueous composition) is then reduced to release the cation from the multi-dentate ligand; and the multi-dentate ligand, after the cation is released, is then reused in a predominantly closed loop. | 09-18-2014 |
20140367871 | Multi-Stage Bubble Column Humidifier - A feed liquid flows into a second-stage humidifier chamber to form a second-stage humidifier bath. A first remnant of the feed liquid from the second-stage humidifier chamber then flows into a first-stage humidifier chamber to form a first-stage humidifier bath having a temperature lower than that of the second-stage bath. A second remnant of the feed liquid is then removed from the first-stage humidifier. Meanwhile, a carrier gas is injected into and bubbled through the first-stage humidifier bath, collecting a vaporizable component in vapor form from the first remnant of the feed liquid to partially humidify the carrier gas. The partially humidified carrier gas is then bubbled through the second-stage humidifier bath, where the carrier gas collects more of the vaporizable component in vapor form from the feed liquid to further humidify the carrier gas before the humidified carrier gas is removed from the second-stage humidifier chamber. | 12-18-2014 |
20150321118 | Multi-Stage Bubble Column Humidification and Dehumidification - A feed liquid flows into a second-stage humidifier chamber to form a second-stage humidifier bath. A first remnant of the feed liquid from the second-stage humidifier chamber then flows into a first-stage humidifier chamber to form a first-stage humidifier bath having a temperature lower than that of the second-stage bath. A second remnant of the feed liquid is then removed from the first-stage humidifier. Meanwhile, a carrier gas is injected into and bubbled through the first-stage humidifier bath, collecting a vaporizable component in vapor form from the first remnant of the feed liquid to partially humidify the carrier gas. The partially humidified carrier gas is then bubbled through the second-stage humidifier bath, where the carrier gas collects more of the vaporizable component in vapor form from the feed liquid to further humidify the carrier gas before the humidified carrier gas is removed from the second-stage humidifier chamber. | 11-12-2015 |
20150368121 | Multi-Stage Bubble Column Humidifier - A feed liquid flows into a second-stage humidifier chamber to form a second-stage humidifier bath. A first remnant of the feed liquid from the second-stage humidifier chamber then flows into a first-stage humidifier chamber to form a first-stage humidifier bath having a temperature lower than that of the second-stage bath. A second remnant of the feed liquid is then removed from the first-stage humidifier. Meanwhile, a carrier gas is injected into and bubbled through the first-stage humidifier bath, collecting a vaporizable component in vapor form from the first remnant of the feed liquid to partially humidify the carrier gas. The partially humidified carrier gas is then bubbled through the second-stage humidifier bath, where the carrier gas collects more of the vaporizable component in vapor form from the feed liquid to further humidify the carrier gas before the humidified carrier gas is removed from the second-stage humidifier chamber. | 12-24-2015 |
Patent application number | Description | Published |
20150060286 | WATER TREATMENT SYSTEMS AND ASSOCIATED METHODS - Water treatment systems and associated methods are generally described. Certain of the water treatment systems and methods described herein can be used to pre-treat an aqueous stream that is subsequently transported to a desalination apparatus and/or post-treat an aqueous stream that has been produced by a desalination apparatus. | 03-05-2015 |
20150083577 | DESALINATION SYSTEMS AND ASSOCIATED METHODS - Systems and methods related to the desalination of aqueous solutions containing one or more dissolved salts are generally described. | 03-26-2015 |
20150129410 | SYSTEMS INCLUDING A CONDENSING APPARATUS SUCH AS A BUBBLE COLUMN CONDENSER - Condensing apparatuses and their use in various heat and mass exchange systems are generally described. The condensing apparatuses, such as bubble column condensers, may employ a heat exchanger positioned external to the condensing vessel to remove heat from a bubble column condenser outlet stream to produce a heat exchanger outlet stream. In certain cases, the condensing apparatus may also include a cooling device positioned external to the vessel configured and positioned to remove heat from the heat exchanger outlet stream to produce a cooling device outlet stream. The condensing apparatus may be configured to include various internal features, such as a vapor distribution region and/or a plurality of liquid flow control weirs and/or chambers within the apparatus having an aspect ratio of at least 1.5. A condensing apparatus may be coupled with a humidifier to form part of a desalination system, in certain cases. | 05-14-2015 |
20150130093 | SYSTEMS INCLUDING A CONDENSING APPARATUS SUCH AS A BUBBLE COLUMN CONDENSER - Condensing apparatuses and their use in various heat and mass exchange systems are generally described. The condensing apparatuses, such as bubble column condensers, may employ a heat exchanger positioned external to the condensing vessel to remove heat from a bubble column condenser outlet stream to produce a heat exchanger outlet stream. In certain cases, the condensing apparatus may also include a cooling device positioned external to the vessel configured and positioned to remove heat from the heat exchanger outlet stream to produce a cooling device outlet stream. The condensing apparatus may be configured to include various internal features, such as a vapor distribution region and/or a plurality of liquid flow control weirs and/or chambers within the apparatus having an aspect ratio of at least 1.5. A condensing apparatus may be coupled with a humidifier to form part of a desalination system, in certain cases. | 05-14-2015 |
20150190730 | SYSTEMS INCLUDING A CONDENSING APPARATUS SUCH AS A BUBBLE COLUMN CONDENSER - Condensing apparatuses and their use in various heat and mass exchange systems are generally described. The condensing apparatuses, such as bubble column condensers, may employ a heat exchanger positioned external to the condensing vessel to remove heat from a bubble column condenser outlet stream to produce a heat exchanger outlet stream. In certain cases, the condensing apparatus may also include a cooling device positioned external to the vessel configured and positioned to remove heat from the heat exchanger outlet stream to produce a cooling device outlet stream. The condensing apparatus may be configured to include various internal features, such as a vapor distribution region and/or a plurality of liquid flow control weirs and/or chambers within the apparatus having an aspect ratio of at least 1.5. A condensing apparatus may be coupled with a humidifier to form part of a desalination system, in certain cases. | 07-09-2015 |
20150190731 | SYSTEMS INCLUDING A CONDENSING APPARATUS SUCH AS A BUBBLE COLUMN CONDENSER - Condensing apparatuses and their use in various heat and mass exchange systems are generally described. The condensing apparatuses, such as bubble column condensers, may employ a heat exchanger positioned external to the condensing vessel to remove heat from a bubble column condenser outlet stream to produce a heat exchanger outlet stream. In certain cases, the condensing apparatus may also include a cooling device positioned external to the vessel configured and positioned to remove heat from the heat exchanger outlet stream to produce a cooling device outlet stream. The condensing apparatus may be configured to include various internal features, such as a vapor distribution region and/or a plurality of liquid flow control weirs and/or chambers within the apparatus having an aspect ratio of at least 1.5. A condensing apparatus may be coupled with a humidifier to form part of a desalination system, in certain cases. | 07-09-2015 |
Patent application number | Description | Published |
20090312512 | HIGH MOLECULAR WEIGHT COMPACT STRUCTURED POLYMERS, METHODS OF MAKING AND USING - A structured water soluble polymer produced by polymerization of amine-containing monomers or pre-formed polymers is described. Various structured polymers are prepared and characterized. The structured polymers are particularly valued in the papermaking industry. | 12-17-2009 |
20100089542 | ALDEHYDE-FUNCTIONALIZED POLYMERS - A composition for enhancing the dewatering of a paper sheet on a paper machine and a method for using the composition is disclosed. The composition comprising one or more aldehyde-functionalized polymers comprising amino or amido groups wherein at least about 15 mole percent of the amino or amido groups are functionalized by reacting with one or more aldehydes and wherein the aldehyde functionalized polymers have a molecular weight of at least about 100,000. The method comprising adding the composition to the paper machine at a dosage of about 0.05 lb/ton to about 15 lb/ton, based on dry fiber. | 04-15-2010 |
20110146925 | ALDEHYDE-FUNCTIONALIZED POLYMERS WITH ENHANCED STABILITY - The current invention includes a composition having stability additives of selected inorganic salts and/or organic incorporated into an aldehyde-functionalized polymer product. The stability additive can be post added to the finished polymer product, added to the reaction vessel prior to inducing functionalization of the non-functionalized polymer, or added at any stage during the functionalization reaction. Methods for forming the composition and using the composition to produce a cellulosic fiber-based medium are also disclosed. | 06-23-2011 |
20120103547 | METHOD OF USING ALDEHYDE-FUNCTIONALIZED POLYMERS TO INCREASE PAPERMACHINE PERFORMANCE AND ENHANCE SIZING - Novel sizing mixtures to achieve improved sizing along with other benefits is disclosed and claimed. The invention is a composition comprising a sizing mixture having a stabilizing amount of one or more aldehyde-functionalized polymers and a sizing amount of a sizing composition. The invention is also a method of improving paper and paperboard production and enhancing sizing through adding an effective amount of the disclosed sizing mixture to the paper machine and a method of producing a medium having cellulosic fibers, wherein the method includes adding the disclosed sizing mixture to the medium at any point in a papermaking process. | 05-03-2012 |
20130192782 | METHOD OF USING ALDEHYDE-FUNCTIONALIZED POLYMERS TO INCREASE PAPERMACHINE PERFORMANCE AND ENHANCE SIZING - Disclosed herein are compositions and methods to increase paper machine performance and enhance sizing. The compositions include a sizing agent, an emulsifier, and an aqueous component. The emulsifier may be an aldehyde-functionalized polymer. | 08-01-2013 |
20140060763 | ALDEHYDE-FUNCTIONALIZED POLYMERS WITH ENHANCED STABILITY - The current invention includes a composition having stability additives of selected inorganic salts and/or organic incorporated into an aldehyde-functionalized polymer product. The stability additive can be post added to the finished polymer product, added to the reaction vessel prior to inducing functionalization of the non-functionalized polymer, or added at any stage during the functionalization reaction. Methods for forming the composition and using the composition to produce a cellulosic fiber-based medium are also disclosed. | 03-06-2014 |
20140130994 | EMULSIFICATION OF ALKENYL SUCCINIC ANHYDRIDE WITH AN AMINE-CONTAINING HOMOPOLYMER OR COPOLYMER - The present disclosure provides for a method of preparing and using an emulsion for treating a papermaking process. The emulsion is an oil-in-water emulsion of alkenyl succinic anhydride emulsified with a polymer comprising at least one primary or secondary amine containing monomer. The method comprises adding an oil-in-water emulsion to the papermaking process; wherein the oil-in-water emulsion comprises alkenyl succinic anhydride emulsified with a polymer comprising at least one primary or secondary amine containing monomer; and wherein the oil-in-water emulsion is added in an amount sufficient to improve sizing of the paper produced by the papermaking process. The primary or secondary amine may be a secondary amine comprising diallylamine, and the polymer may be a diallylamine-acrylamide copolymer. | 05-15-2014 |
20140336314 | METHOD OF USING ALDEHYDE-FUNCTIONALIZED POLYMERS TO INCREASE PAPERMACHINE PERFORMANCE AND ENHANCE SIZING - Disclosed herein are compositions and methods to increase paper machine performance and enhance sizing. The compositions include a sizing agent, an emulsifier, and an aqueous component. The emulsifier may be an aldehyde-functionalized polymer. The sizing agent may be selected from rosin size, an alkyl ketene dimer, an alkenyl succinic anhydride, and any combination of these sizing agents. | 11-13-2014 |
20150020988 | Emulsification of Alkenyl Succinic Anhydride with an Amine-Containing Homopolymer of Copolymer - The present disclosure provides for a method of preparing and using an emulsion for treating a papermaking process. The emulsion is an oil-in-water emulsion of alkenyl succinic anhydride emulsified with a polymer comprising at least one primary or secondary amine containing monomer. The method comprises adding an oil-in-water emulsion to the papermaking process; wherein the oil-in-water emulsion comprises alkenyl succinic anhydride emulsified with a polymer comprising at least one primary or secondary amine containing monomer; and wherein the oil-in-water emulsion is added in an amount sufficient to improve sizing of the paper produced by the papermaking process. The primary or secondary amine may be a secondary amine comprising diallylamine, and the polymer may be a diallylamine-acrylamide copolymer. | 01-22-2015 |
Patent application number | Description | Published |
20140160474 | OPTICAL REFLECTORS FOR SPECTROMETER GAS CELLS - A spectrometer cell can include a spacer, at least one end cap, and at least one mirror with a reflective surface. The end cap can be positioned proximate to a first contact end of the spacer such that the end cap and spacer at least partially enclose an internal volume of the spectrometer cell. The mirror can be secured in place by a mechanical attachment that includes attachment materials that are chemically inert to at least one reactive gas compound. The mechanical attachment can hold an optical axis of the reflective surface in a fixed orientation relative to other components of the spectrometer cell and or a spectrometer device that comprises the spectrometer cell. The mirror can optionally be constructed of a material such as stainless steel, ceramic, or the like. Related methods, articles of manufacture, systems, and the like are described. | 06-12-2014 |
20160084710 | OPTICAL REFLECTORS FOR SPECTROMETER GAS CELLS - A spectrometer cell can include a spacer, at least one end cap, and at least one mirror with a reflective surface. The end cap can be positioned proximate to a first contact end of the spacer such that the end cap and spacer at least partially enclose an internal volume of the spectrometer cell. The mirror can be secured in place by a mechanical attachment that includes attachment materials that are chemically inert to at least one reactive gas compound. The mechanical attachment can hold an optical axis of the reflective surface in a fixed orientation relative to other components of the spectrometer cell and or a spectrometer device that comprises the spectrometer cell. The mirror can optionally be constructed of a material such as stainless steel, ceramic, or the like. Related methods, articles of manufacture, systems, and the like are described. | 03-24-2016 |
Patent application number | Description | Published |
20100149020 | DETECTING ROADWAY TARGETS ACROSS BEAMS - The present invention extends to methods, systems, and computer program products for detecting targets across beams at roadway intersections. Embodiments of the invention include tracking a target across a plurality of beams of a multiple beam radar system in a roadway intersection and updating track files for targets within a roadway intersection. Returns from a plurality of radar beams monitoring a roadway intersection are divided into range bins. Identified energy in the range bins is used to compute the position of targets within a roadway intersection. When the position of a target is computed, it is determined if the position is a new position for an existing target or if the position is the position of a new target. | 06-17-2010 |
20140139361 | DETECTING ROADWAY TARGETS ACROSS BEAMS - The present invention extends to methods, systems, and computer program products for detecting targets across beams at roadway intersections. Embodiments of the invention include tracking a target across a plurality of beams of a multiple beam radar system in a roadway intersection and updating track files for targets within a roadway intersection. Returns from a plurality of radar beams monitoring a roadway intersection are divided into range bins. Identified energy in the range bins is used to compute the position of targets within a roadway intersection. When the position of a target is computed, it is determined if the position is a new position for an existing target or if the position is the position of a new target. | 05-22-2014 |
20160086485 | DETECTING ROADWAY TARGETS ACROSS BEAMS - The present invention extends to methods, systems, and computer program products for detecting targets across beams at roadway intersections. Embodiments of the invention include tracking a target across a plurality of beams of a multiple beam radar system in a roadway intersection and updating track files for targets within a roadway intersection. Returns from a plurality of radar beams monitoring a roadway intersection are divided into range bins. Identified energy in the range bins is used to compute the position of targets within a roadway intersection. When the position of a target is computed, it is determined if the position is a new position for an existing target or if the position is the position of a new target. | 03-24-2016 |
Patent application number | Description | Published |
20090054628 | METHOD AND SYSTEM FOR IN VITRO PROTEIN FOLDING - A method of recovering a refolded protein involves static mixing a concentrated solution of a denatured protein with a refolding diluent to obtain the refolded protein. The method is particularly suitable for microbially produced recombinant proteins in large processing volumes. The denatured protein solution can be obtained by isolating protein from the microbial host and exposing them to a denaturant. This solution is mixed with a suitable refolding diluent under static mixing conditions compatible with proper folding of the protein so that the refolded protein is obtained, preferably rapidly and with high yield. A system for implementing the refolded protein recovery method includes a static mixer, a conduit inline with and upstream from the static mixer, and an inlet to the conduit upstream of the static mixer, and optionally a dynamic, preferably non-turbulent, mixing vessel downstream from the static mixer. The invention finds particular use in large scale production of proteins, particularly recombinant proteins. | 02-26-2009 |
20100255536 | HIGH PRESSURE REFOLDING OF PROTEIN AGGREGATES AND INCLUSION BODIES - The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates. | 10-07-2010 |
20110046357 | HIGH PRESSURE REFOLDING OF PROTEIN AGGREGATES AND INCLUSION BODIES - The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates. | 02-24-2011 |
Patent application number | Description | Published |
20090000039 | Demolition utility tool - The invention provides a demolition utility tool that can be used in many ways for the demolition of materials and/or for the manipulation of clamps valves, nuts, or other hardware. The tool includes a handle having a head at one end. The head has a longitudinal central plane that bisects the head, a strike contact face, and toothed, stepped grasping jaws that accommodate multiple sizes of material. The demolition tool may include a fixed wrench element and/or a spanner wrench element on the head. The demolition tool may also include a bent end pry bar at a second end of the handle that is offset 90 degrees from the longitudinal central plane of the tool head enabling full range of motion without interfering with other tool functions. The bent end pry bar may include a slot between located between its forked end for manipulating gas shut-off valves. | 01-01-2009 |
20090193939 | Ratchet Wrench - A wrench adapted to engage a workpiece to drive the workpiece rotationally. In one embodiment, the wrench comprises a head, a first jaw, and a second jaw. The first jaw carried by the head and forms a first workpiece engaging surface. The second jaw is carried by the head and forms a second workpiece engaging surface such that (i) if the head is rotated about a workpiece disposed between the first workpiece engaging surface and the second workpiece engaging surface in a first rotational direction, the first workpiece engaging surface and the second workpiece engaging surface rotationally drive the workpiece in the first rotational direction, and (ii) if the head is rotated about the workpiece disposed between the first workpiece engaging surface and the second workpiece engaging surface in a second rotational direction opposite from the first rotational direction, the first workpiece engaging surface and the second workpiece engaging surface slide along the workpiece as the head rotates in the second rotational direction independent from the workpiece. | 08-06-2009 |
20090193941 | Ratchet Wrench - A wrench adapted to engage a workpiece to drive the workpiece rotationally. In one embodiment, the wrench comprises a handle, a head, a first jaw, a track, a second jaw, and a biasing member. The first jaw is disposed on the head such that the position of the first jaw is adjustable toward and away from the jaw support along a first path, thereby enabling the wrench to be adapted for engagement with workpieces of different sizes. The second jaw is slidably seated in the track formed on the jaw support to slide along a second path between a first position and a second position. The second jaw further cooperates with the first jaw to grasp a workpiece therebetween and drive the workpiece in a first rotational direction if the head is driven in the first rotational direction by the handle. If the head is driven by the handle in a second rotational direction the engagement between the second jaw and the workpiece applies a force to the second jaw that slides the second jaw from the first position toward the second position, which enables the head to be rotated independently from the workpiece in the second rotational direction | 08-06-2009 |
20100039801 | ILLUMINATION DEVICE WITH DETACHABLE LIGHT SOURCES - An illumination device includes independent light sources and pivotable legs. In an embodiment, each independent light source includes a head having an illumination element; a body; a power source holder configured to receive a power source for powering the illumination element; a switch configured to control the independent light source; and an electrical circuit configured to place the independent light source and at least one other independent light source in an electrically coupled state. The switch may be configured to control an illumination state of both the independent light source and the at least one other independent light source when in the electrically coupled state. The illumination provided by the illumination device may be adjusted to different angles with respect to a longitudinal axis of the illumination device by rotating each of the plurality of pivotable heads. The pivotable legs are movable between a retracted configuration and an expanded configuration. | 02-18-2010 |
Patent application number | Description | Published |
20090240326 | Implant Delivery and Deployment System and Method - An implant delivery system comprising a catheter including at least one lumen, an implant configured to be received in the lumen, and a latching mechanism configured to be received in the implant. The latching mechanism may be configured to releasably couple the implant to a delivery wire and to transmit a torque through the delivery wire to cause at least a portion of the implant to rotate. The an implant may comprise a shaft, a spacer configured to interact with at least a portion of at least one cusp of a heart valve to at least partially restrict a flow of blood through the heart valve in a closed position, a garage configured to couple the spacer to a first end region of the shaft, and at least one anchor mechanism. The garage may define a cavity configured to receive the latching mechanism and to increase rotational and translational stability of the latching mechanism. | 09-24-2009 |
20100022948 | Steerable Catheter and Dilator and System and Method for Implanting a Heart Implant - A catheter according to one embodiment may be configured to extend through a transseptal puncture site, a left atrium, and into a left ventricle. The catheter may comprise a shaft defining at least one lumen configured to receive at least one of an implant and/or a dilator, a first steerable actuator coupled to the shaft at a position on the shaft substantially corresponding to the annulus of the mitral valve when the distal end of the shaft is disposed within the left ventricle, and a handle assembly coupled to a proximal end of the shaft. The handle assembly may comprise a first actuator coupled to the first steerable actuator. The first actuator may be configured to apply a force to the first steerable actuator to deflect the shaft about a region proximate to the first steerable actuator. | 01-28-2010 |
20130041459 | Implant Delivery and Deployment System and Method - An implant delivery system comprising a catheter including at least one lumen, an implant configured for receipt in the lumen, and a latching mechanism configured for receipt in the implant. The latching mechanism may be configured to releasably couple the implant to a delivery wire and to transmit torque through the delivery wire to cause at least a portion of the implant to rotate. The an implant may comprise a shaft, a spacer configured to interact with at least a portion of at least one cusp of a heart valve to at least partially restrict a flow of blood through the heart valve in a closed position, a garage configured to couple the spacer to a first end region of the shaft, and at least one anchor mechanism. The garage may define a cavity to receive the latching mechanism and to increase rotational and translational stability of the latching mechanism. | 02-14-2013 |
20140207230 | Implant Delivery and Deployment System and Method - An implant delivery system comprising a catheter including at least one lumen, an implant configured to be received in the lumen, and a latching mechanism configured to be received in the implant. The latching mechanism may be configured to releasably couple the implant to a delivery wire and to transmit a torque through the delivery wire to cause at least a portion of the implant to rotate. The an implant may comprise a shaft, a spacer configured to interact with at least a portion of at least one cusp of a heart valve to at least partially restrict a flow of blood through the heart valve in a closed position, a garage configured to couple the spacer to a first end region of the shaft, and at least one anchor mechanism. The garage may define a cavity configured to receive the latching mechanism and to increase rotational and translational stability of the latching mechanism. | 07-24-2014 |
20140276395 | Steerable Catheter and Dilator and System and Method for Implanting a Heart Implant - A catheter according to one embodiment may be configured to extend through a transseptal puncture site, a left atrium, and into a left ventricle. The catheter may comprise a shaft defining at least one lumen configured to receive at least one of an implant and/or a dilator, a first steerable actuator coupled to the shaft at a position on the shaft substantially corresponding to the annulus of the mitral valve when the distal end of the shaft is disposed within the left ventricle, and a handle assembly coupled to a proximal end of the shaft. The handle assembly may comprise a first actuator coupled to the first steerable actuator. The first actuator may be configured to apply a force to the first steerable actuator to deflect the shaft about a region proximate to the first steerable actuator. | 09-18-2014 |
Patent application number | Description | Published |
20100024109 | Toilet splash guard - The present invention is directed to a splash guard for containing the contents of a toilet during plunging with a plunger. The splash guard comprises a pliable shield having a top side, a bowl side, an upper opening, a lower opening, an elastic element and a drape member, the upper opening having a periphery sized and shaped for receiving the underside of a toilet seat, the elastic element disposed along the periphery for enveloping the underside of the toilet seat by overlapping with a portion of the upper side of the toilet seat, thereby retaining the splash guard in position relative to the toilet seat, whereby when the toilet seat is in a down position, the top side faces up and the bowl side faces the toilet bowl. The lower opening is sized for slideably receiving the elongated handle of the plunger, the drape member attached in proximity to the periphery and when the toilet seat is down, draping sufficiently into the toilet bowl so as to shield the rim from receiving splashes or waste during plunging with the plunger. | 02-04-2010 |
20100024110 | Toilet splash guard - The present invention is directed a splash guard for containing the contents of a toilet during plunging, comprising: a substantially ovular pliable shield having a top side, a bottom side, a peripheral edge and a blunted posterior end, the pliable shield having a size from 5-20% greater than the size of said toilet seat; a drape member having an upper edge, a bottom edge, and a distance between the upper edge and said bottom edge of 3 to 8 inches; and a resilient element; the top edge of the drape member circumferentially affixed to the peripheral edge of the pliable shield at a seam, the resilient element being tensioned and operatively affixed along the length of the seam but optionally the elastic element not being affixed to the seam at the blunted end; the pliable shield further having a minor opening sized and positioned for slideably receiving a plunger handle, whereby when the splash guard is positioned on the underside of the toilet seat and the resilient element is stretched outwardly, and then upwardly to engage the top of the toilet seat, the top of the pliable shield covers the bottom of the toilet seat and is held in position by the tension in the resilient member, and when the toilet seat is in the down position, the drape member drapes down sufficiently into the toilet bowl and in proximity to the rim so as to shield the rim from receiving splashes or waste during plunging with the plunger. | 02-04-2010 |