Patent application number | Description | Published |
20090011468 | Method for improving protein production - The present invention relates to the production of proteins in a cell or host cell. The invention uses a TRAnscription Pause (TRAP) sequence to enhance a protein expression characteristic of a protein expression unit. The TRAP sequence is thought to prevent, at least in part, formation of antisense RNA or to, at least in part, prevent transcription to enter the protein expression unit. In one embodiment, the invention provides a method for expression of at least one protein of interest in a cell comprising providing the cell with at least one protein expression unit that comprises a promoter functionally linked to an open reading frame encoding at least one protein of interest, characterized in that the protein expression unit further comprises at least one TRAP sequence and wherein the TRAP sequence is functionally located downstream of the open reading frame and at least in part prevents formation of antisense RNA. In another embodiment, the TRAP sequence is functionally located upstream of the promoter and at least in part prevents transcription to enter the expression unit. Preferably, the expression protein unit further comprises at least one STabilizing Anti-Repressor sequence. | 01-08-2009 |
20090098601 | Selection of Host Cells Expressing Protein at High Levels - The invention provides a DNA molecule comprising an open reading frame sequence that encodes a selectable marker polypeptide, wherein said DNA molecule in the coding strand comprises a translation start sequence for the selectable marker polypeptide having a GTG startcodon or a TTG startcodon, and wherein the open reading frame sequence that encodes the selectable marker protein has been mutated to replace at least half of its CpG dinucleotides as compared to the native open reading frame sequence that encodes the selectable marker protein. | 04-16-2009 |
20100136616 | Selection of Host Cells Expressing Protein at High Levels - The invention provides a DNA molecule comprising a multicistronic transcription unit coding for i) a polypeptide of interest, and for ii) a selectable marker polypeptide functional in a eukaryotic host cell, wherein the polypeptide of interest has a translation initiation sequence separate from that of the selectable marker polypeptide, and wherein the coding sequence for the polypeptide of interest is upstream from the coding sequence for the selectable marker polypeptide in said multicistronic transcription unit, and wherein an internal ribosome entry site (IRES) is present downstream from the coding sequence for the polypeptide of interest and upstream from the coding sequence for the selectable marker polypeptide, and wherein the nucleic acid sequence coding for the selectable marker polypeptide in the coding strand comprises a GTG or a TTG startcodon. The invention also provides methods for obtaining host cells expressing a polypeptide of interest, said host cells comprising the DNA molecules of the invention. The invention further provides the production of polypeptides of interest, comprising culturing host cells comprising the DNA molecules according to the invention. | 06-03-2010 |
20100190207 | Expression Augmenting DNA Fragments, Use Thereof, and Methods for Finding Thereof - The invention provides recombinant DNA molecules comprising novel expression augmenting DNA fragments and an expression cassette, said expression cassette comprising a heterologous promoter linked to a nucleic acid of interest. The invention further provides uses of the novel expression augmenting DNA fragments. The invention further provides methods for obtaining novel expression augmenting DNA fragments. | 07-29-2010 |
20110300580 | Selection of host cells expressing protein at high levels - Described is a DNA molecule comprising an open reading frame sequence that encodes a selectable marker polypeptide, wherein the DNA molecule in the coding strand comprises a translation start sequence for the selectable marker polypeptide having a GTG start codon or a TTG start codon, and wherein the ORF sequence that encodes the selectable marker protein has been mutated to replace at least half of its CpG dinucleotides as compared to the native ORF sequence that encodes the selectable marker protein. Further provided are such DNA molecules wherein the ORF sequence that encodes a selectable marker polypeptide is part of a multicistronic transcription unit that further comprises an open reading frame sequence encoding a polypeptide of interest. Also described are methods for obtaining host cells expressing a polypeptide of interest, wherein the host cells comprise the DNA molecules described herein. Further provided is the production of polypeptides of interest, comprising culturing host cells comprising the DNA molecules described herein. | 12-08-2011 |
Patent application number | Description | Published |
20100080879 | Method and device for arranging a dough layer on a conveyor - The invention relates to a device for arranging a dough layer on a conveyor, comprising a funnel with a receiving chamber for receiving dough, a discharge opening into which the receiving chamber debouches, and means for applying an oil film to at least a part of the inner side of the receiving chamber, wherein at least a part of the inner wall of the receiving chamber is convex on the interior of the funnel in a section parallel to a direction of the receiving chamber toward the discharge opening. The invention also relates to a method for arranging a dough layer on a conveyor. | 04-01-2010 |
20100183784 | Method and Device for Arranging a Sprinkling Material on a Dough Piece - The present invention relates to a method and device for arranging sprinkling material, such as seed, on a dough piece, comprising means for arranging the sprinkling material on a surface for dough pieces and means for placing the dough piece on the surface provided with the sprinkling material, and also further comprising in an advantageous embodiment means for applying an adhesive such as water to at least a part of the dough piece to be provided with the sprinkling material, wherein the means for placing the dough piece are adapted to place the dough piece with the part provided with the adhesive facing toward the surface. | 07-22-2010 |
20100247729 | Method and Device for Curling Up Dough Slices - The present invention relates to a method and device for curling up dough slices, comprising a conveyor for the dough slices, a gripping element, arranged above the conveyor, for curling up a dough slice conveyed under the gripping element by the conveyor, pressure means, for pressing curled up dough slices on the conveyor, wherein the distance along which the gripping element grips dough slices is adjustable in the direction of conveyance. | 09-30-2010 |
20100297319 | Device and method for twisting elongated dough strips - The present invention relates to a device for twisting elongated dough strips, comprising at least one pair of grippers, each arranged for gripping and respectively releasing one short side of a dough strip, and means for mutually rotating the grippers around an axis of rotation. In an embodiment, the device comprises a first conveyor for supplying dough strips oriented with their long sides perpendicular to the direction of conveyance wherein the width of the first conveyor is chosen smaller than the long sides of the dough strips so that the short sides of a dough strip protrude from the first conveyor, and a second conveyor for carrying off the twisted dough strips. The invention further provides a method for twisting an elongated dough strip. | 11-25-2010 |
20110097467 | Device and Method for Pinching at Least Two Dough Parts - The present invention relates to a device comprising a conveyor for conveying the dough parts wherein the pair of drivable pinchers is arranged along the conveyor movable with at least a directional component towards each other and wherein the sensor is arranged upstream in the direction of conveyance with respect to the pair of pincers. The invention further relates to a method for pinching at least two dough parts, comprising conveying the dough parts to a pinching location, detecting the presence of the dough parts by sensing means upstream with respect to the pinching location in a conveying direction and operating pinchers upon detection of the dough parts. | 04-28-2011 |
20120308693 | Dough Piece Rolled Up with a Separator Sheet, and Method and Device for Preparing These - The present invention leads to a method for preparing a dough piece rolled up with separator sheet, comprising supplying in a transport direction, a dough piece positioned on a separator sheet, the dough piece and the separator sheet having a length and a width, wherein a leading edge of the separator sheet exceeds the dough piece at at least a first side, rolling up the at least one side of the dough paper that exceeds the dough piece, thus forming a cylinder of separator sheet, extending in a width direction of the dough piece and rolling up the rest of the separator sheet with the dough piece around the cylinder. The invention further relates to a device for performing the method and a roll formed by the method and/or device. | 12-06-2012 |
20130283988 | Device for Cutting Dough - The present invention relates to a device for cutting dough, comprising, a conveyor for conveying an elongated or endless dough piece in an essentially horizontal direction; a slide, arranged translatable in the essentially horizontal direction of conveyance of the conveyor, a cutting blade, extending horizontally and essentially perpendicular to the direction of conveyance of the conveyor, and coupled to the slide, translatable in an essentially vertical direction, a moment, having an arm extending essentially vertically, with a first arm part extending above a fulcrum, and a second arm part extending below the fulcrum, the first arm part coupled to the slide, and the second arm part coupled to a first drive for rocking the arm about the fulcrum, and a second drive, for translating the cutting blade in the essentially vertical direction with respect to the slide. | 10-31-2013 |
20140020530 | Method and Device for Treating Dough - The present invention relates to a device for treating dough, comprising an essentially horizontally extending conveyor, for conveying a flat dough sheet, at least one mat, arranged above the conveyor, with its end downstream the direction of conveyance resting on or slightly above the conveyor, for rolling up the dough sheet when it is conveyed underneath, at least one fence, forming a blunt sealing knife, arranged vertically at a distance above the conveyor surface and extending in the direction of conveyance, downstream the at least one mat, for notching and therewith sealing the rolled up dough sheet without cutting it and at least one rotatable knife, arranged at the downstream outermost end of the fence and in the plane of said fence, for cutting the notched dough piece. The invention further relates to a method for treating dough. | 01-23-2014 |
20140034448 | Device for Weighing Dough - The present invention relates to a device for weighing dough, comprising an endless conveyor, for conveying a plurality of endless dough pieces, extending essentially in parallel lanes on said conveyor in a direction of conveyance and a plurality of weighing-units, arranged under the endless conveyor, and supporting the latter, the weighing units being distributed at different locations spread over the width of the conveyor, each for weighing a different dough piece. | 02-06-2014 |
20140050825 | Device for Providing Dough Products with a Topping Material - Method and device for providing dough products with a topping material, comprising a dough product conveyor, for transporting the dough products, a topping dispenser for dispensing topping material onto the dough product conveyor, comprising a topping conveyor, for conveying the topping towards a dispensing location above the dough product conveyor, a collector for superfluous topping material from the dough product conveyor, a supply system, for supplying the topping conveyor with topping material from the collector or from a reservoir with fresh topping material, characterised by a topping material sensor for measuring the amount of topping material on the topping conveyor, a controller, for controlling the amount of topping material to be dispensed by adjusting the speed of the topping conveyor; and the ratio of topping material from the reservoir and the collected superfluous topping material. | 02-20-2014 |
20150064315 | Device for Weighing Dough and Method for Operating such Device - The present invention relates to a device for weighing dough, comprising an endless conveyor, for conveying a plurality of endless or continuous dough pieces extending essentially in parallel lanes on said conveyor in a direction of conveyance, a weighing-unit, arranged under the endless conveyor, characterised in that the weighing unit comprises multiple weighing rows, spread over the width of the conveyor, each row comprising at least one rectangular weighing section, for independently of the other weighing sections weighing a different dough piece of said plurality of endless or continuous dough pieces. The invention further relates to a method for operating such device. | 03-05-2015 |