Patent application number | Description | Published |
20090011402 | BIOSENSORS BASED ON DIRECTED ASSEMBLY OF PARTICLES - A sensor system for detecting an effector or cofactor comprises (a) a nucleic acid enzyme; (b) a substrate for the nucleic acid enzyme, comprising a first polynucleotide; (c) a first set of particles comprising a second polynucleotide at least partially complementary to the substrate, where the polynucleotide is attached to the particles at its 3′ terminus; and (d) a second set of particles comprising a third polynucleotide at least partially complementary to the substrate, where the polynucleotide is attached to the particles at its 5′ terminus. | 01-08-2009 |
20090029874 | FLUORESCENCE BASED BIOSENSOR - A novel biosensor comprises at least one fluorophore and at least two quenchers, and is capable of selectively and specifically detecting the presence of an ion in the presence of other ions. | 01-29-2009 |
20090098550 | MRI CONTRAST AGENTS AND HIGH-THROUGHPUT SCREENING BY MRI - The present invention provides an MRI contrast agent, comprising: MRI contrast agent particles, and oligonucleotides, attached to the particles. | 04-16-2009 |
20090143239 | FLUORESCENCE BASED BIOSENSOR - A novel biosensor comprises at least one fluorophore and at least two quenchers, and is capable of selectively and specifically detecting the presence of an ion in the presence of other ions. | 06-04-2009 |
20090197261 | APTAMER- AND NUCLEIC ACID ENZYME-BASED SYSTEMS FOR SIMULTANEOUS DETECTION OF MULTIPLE ANALYTES - The present invention provides aptamer- and nucleic acid enzyme-based systems for simultaneously determining the presence and optionally the concentration of multiple analytes in a sample. Methods of utilizing the system and kits that include the sensor components are also provided. The system includes a first reactive polynucleotide that reacts to a first analyte; a second reactive polynucleotide that reacts to a second analyte; a third polynucleotide; a fourth polynucleotide; a first particle, coupled to the third polynucleotide; a second particle, coupled to the fourth polynucleotide; and at least one quencher, for quenching emissions of the first and second quantum dots, coupled to the first and second reactive polynucleotides. The first particle includes a quantum dot having a first emission wavelength. The second particle includes a second quantum dot having a second emission wavelength different from the first emission wavelength. The third polynucleotide and the fourth polynucleotide are different. | 08-06-2009 |
20100105039 | LABEL-FREE COLORIMETRIC DETECTION - The present invention provides a sensor system kit for detecting an analyte, consisting essentially of: a nucleic acid enzyme, wherein the nucleic acid enzyme cleaves a substrate in the presence of the analyte; the substrate for the nucleic acid enzyme, comprising a polynucleotide; an aggregator; and particles. | 04-29-2010 |
20100151579 | FLUORESCENT SENSOR FOR MERCURY - The present invention provides a sensor for detecting mercury, comprising: a first polynucleotide, comprising a first region, and a second region, a second polynucleotide, a third polynucleotide, a fluorophore, and a quencher, wherein the third polynucleotide is optionally linked to the second region; the fluorophore is linked to the first polynucleotide and the quencher is linked to the second polynucleotide, or the fluorophore is linked to the second polynucleotide and the quencher is linked to the first polynucleotide; the first region and the second region hybridize to the second polynucleotide; and the second region binds to the third polynucleotide in the presence of Hg | 06-17-2010 |
20100166842 | AMPHIPHILIC SUBSTANCES AND FUNCTIONALIZED LIPID VESICLES INCLUDING THE SAME - An amphiphilic substance includes a hydrophobic group, and a polynucleotide group attached to the hydrophobic group. The polynucleotide group includes a first polynucleotide segment and a second polynucleotide segment. The first and second polynucleotide segments are at least partially complementary and are bound together by interactions including base pairing. At least one of the first and second segments includes at least one of an aptamer and a nucleic acid-based enzyme. A lipid vesicle may include the amphiphilic substance, a first polar lipid that is an unstable vesicle former, and a polar liquid. Upon exposure to a rupture agent, the vesicle may rupture, releasing the contents of the vesicle. Substances that may be released from the vesicle include bioactive agents, such as drug agents. | 07-01-2010 |
20110123982 | NUCLEIC ACID BASED FLUORESCENT SENSOR FOR COPPER DETECTION - A nucleic acid enzyme responsive to copper, comprising an oligonucleotide comprising a nucleotide sequence of SEQ ID NO:1, wherein the nucleic acid enzyme is not self-cleaving. | 05-26-2011 |
20110171635 | NUCLEIC ACID ENZYME BIOSENSORS FOR IONS - Disclosed are compositions and methods for the sensitive and selective detection of ions using nucleic acid enzymes. | 07-14-2011 |
20110236991 | Aptamer-Based Colorimetric Sensor Systems - The present invention provides an aptamer-based colorimetric sensor system for determining the presence and optionally the concentration of an analyte in a sample. Methods of utilizing the sensor system and kits that include the sensor also are provided. The sensor utilizes a linker and oligonucleotide functionalized particles to form an aggregate, which disaggregates in response to the analyte. | 09-29-2011 |
20120107242 | NUCLEIC ACID-MEDIATED SHAPE CONTROL OF NANOPARTICLES FOR BIOMEDICAL APPLICATIONS - Embodiments of a method for nucleic acid-mediated control of a nanoparticle shape are disclosed. In some embodiments, one or more nucleic acid oligomers are adsorbed to a metal nanoseed, and additional metal is deposited onto the nanoseed to produce a shaped nanoparticle. In certain embodiments, the nanoseed is gold and the oligomers are 5-100 nucleotides in length. The nanoparticle shape is determined at least in part by the nucleic acid sequence of the oligomer(s). Shaped nanoparticles produced by embodiments of the method include nanoflowers, nanospheres, nanostars, and nanoplates. Embodiments for using the shaped nanoparticles also are disclosed. | 05-03-2012 |
20120149119 | NUCLEIC ACID BASED FLUORESCENT SENSOR FOR MERCURY DETECTION - A nucleic acid enzyme comprises an oligonucleotide containing thymine bases. The nucleic acid enzyme is dependent on both Hg | 06-14-2012 |
20120167101 | SYSTEM AND METHOD FOR PROACTIVE TASK SCHEDULING - The described implementations relate to distributed computing. One implementation provides a system that can include an outlier detection component that is configured to identify an outlier task from a plurality of tasks based on runtimes of the plurality of tasks. The system can also include a cause evaluation component that is configured to evaluate a cause of the outlier task. For example, the cause of the outlier task can be an amount of data processed by the outlier task, contention for resources used to execute the outlier task, or a communication link with congested bandwidth that is used by the outlier task to input or output data. The system can also include one or more processing devices configured to execute one or more of the components. | 06-28-2012 |
20120252128 | LABEL-FREE FUNCTIONAL NUCLEIC ACID SENSORS FOR DETECTING TARGET AGENTS - A general methodology to design label-free fluorescent functional nucleic acid sensors using a vacant site approach and an abasic site approach is described. In one example, a method for designing label-free fluorescent functional nucleic acid sensors (e.g., those that include a DNAzyme, aptamer or aptazyme) that have a tunable dynamic range through the introduction of an abasic site (e.g., dSpacer) or a vacant site into the functional nucleic acids. Also provided is a general method for designing label-free fluorescent aptamer sensors based on the regulation of malachite green (MG) fluorescence. A general method for designing label-free fluorescent catalytic and molecular beacons (CAMBs) is also provided. The methods demonstrated here can be used to design many other label-free fluorescent sensors to detect a wide range of analytes. Sensors and methods of using the disclosed sensors are also provided. | 10-04-2012 |
20120315621 | PERSONAL GLUCOSE METERS FOR DETECTION AND QUANTIFICATION OF A BROAD RANGE OF ANALYTES - A methodology for developing highly sensitive and selective sensors that can achieve portable, low-cost and quantitative detection of a broad range of targets using only a personal glucose meter (PGM) is disclosed. The method uses recognition molecules specific for a target agent, enzymes that can convert an enzyme substrate into glucose, and a PGM. Also provided are sensors, which can include a solid support having attached thereto a recognition molecule that permits detection of a target agent, as well as an enzyme that can catalyze the conversion of a substance into glucose, wherein the enzyme is attached directly or indirectly to the recognition molecule, and wherein in the presence of the target agent the enzyme can convert the substance into glucose. The disclosed sensors can be part of a lateral flow device. Methods of using such sensors for detecting target agents are provided. | 12-13-2012 |
20130065224 | PERSONAL GLUCOSE METERS FOR DETECTION AND QUANTIFICATION OF A BROAD RANGE OF ANALYTES - A general methodology for the development of highly sensitive and selective sensors that can achieve portable, low-cost and quantitative detection of a broad range of targets using only a personal glucose meter (PGM) is disclosed. The method uses recognition molecules that are specific for a target agent, enzymes that can convert an enzyme substrate into glucose, and PGM. Also provided are sensors, which can include a solid support to which is attached a recognition molecule that permits detection of a target agent, wherein the recognition molecule specifically binds to the target agent in the presence of the target agent but not significantly to other agents as well as an enzyme that can catalyze the conversion of a substance into glucose, wherein the enzyme is attached directly or indirectly to the recognition molecule, and wherein in the presence of the target agent the enzyme can convert the substance into glucose. The disclosed sensors can be part of a lateral flow device. Methods of using such sensors for detecting target agents are also provided. | 03-14-2013 |
20130095039 | NUCLEIC ACID-MEDIATED SHAPE CONTROL OF NANOPARTICLES - Embodiments of a method to use nucleic acid oligomer sequences for modulating the shape of nanoparticles are disclosed, as well as nanoparticles and methods of using the nanoparticles. Systematic variations of the nucleic acid sequences offer mechanistic insights into the morphology control. A plurality of nucleic acid oligomers is adsorbed onto a metal nanoseed to provide an oligomer-functionalized nanoparticle. Additional metal is deposited onto the oligomer-functionalized nanoparticle to produce a shaped nanoparticle having a morphology based at least in part on the nanoseed morphology and the oligomer's sequence composition. Embodiments of methods for using the shaped nanoparticles also are disclosed. | 04-18-2013 |
20140024818 | ALIGNMENT OF NANOMATERIALS AND MICROMATERIALS - The present invention provides a method for preparing a nanoassembly that includes the step of reacting the assembly template with at least one nanomaterial to form the nanoassembly using a bifunctional linker. | 01-23-2014 |
20150024415 | DETECTION AND QUANTIFICATION OF ANALYTES BASED ON SIGNAL INDUCED BY ALKALINE PHOSPHATE - A general methodology for highly sensitive and selective sensors that can achieve portable, low-cost and quantitative detection of a broad range of targets using only a personal glucose meter (PGM) is disclosed. The method and sensors take advantage of the ability of alkaline phosphatase (ALP) to convert glucose-1-phosphate to glucose, and the ability of PGMs to detect the generated glucose. The disclosed sensors can be part of a lateral flow device. Methods of using such sensors for detecting target agents, for example to diagnose disease, are also provided. | 01-22-2015 |
20150031014 | DETECTING ANALYTES WITH A PH METER - Provided herein are sensors, kits that include such sensors, and methods for making and using such sensors. The sensors permit detection of a broad array of target molecules, such as nucleic acids (e.g., DNA and RNA), proteins, toxins, pathogens, cells, and metals, and can be used in combination with pH meters and pH paper. Thus, this disclosure provides a new methodology that allows pH meters and pH paper to be used for the detection of analytes other than pH. | 01-29-2015 |