Patent application number | Description | Published |
20090087074 | METHODS AND SYSTEMS FOR PROCESSING BIOLOGICAL SPECIMENS UTILIZING MULTIPLE WAVELENGTHS - Methods, systems and computer readable media for processing one or more biological specimens carried by specimen slides. Images of objects in a specimen are acquired and objects of interest in the acquired images are identified. Additional images of identified objects of interest may be acquired at multiple wavelengths. Cellular features of objects of interest are extracted from images and may be used for classifying the specimen, e.g., as normal or suspicious/abnormal, based a probabilistic model that utilizes the extracted features. | 04-02-2009 |
20090245612 | AUTOMATED IMAGE ANALYSIS - One automated imaging process, as described herein, includes: a) obtaining digital images of objects in a biological sample; b) selecting a plurality of objects of interest from the digital images; c) obtaining multiple images of the selected objects of interest at a plurality of different wavelengths; d) combining one of said multiple images with a corresponding digital image to produce a combined image; and e) analyzing the combined image in order to characterize the biological sample. | 10-01-2009 |
20090269799 | METHOD OF DETERMINING A COMPLETE BLOOD COUNT AND A WHITE BLOOD CELL DIFFERENTIAL COUNT - Systems and methods analyzing body fluids such as blood and bone marrow are disclosed. The systems and methods may utilize an improved technique for applying a monolayer of cells to a slide to generate a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and methods for utilizing multi color microscopy for improving the quality of images captured by a light receiving device. | 10-29-2009 |
20100021037 | METHOD AND SYSTEM FOR IDENTIFYING BIOLOGICAL SPECIMEN SLIDES USING UNIQUE SLIDE FINGERPRINTS - Method and system for processing specimen slides. A first image of a unique feature, such as a fiducial mark, of a specimen slide is acquired, and a second image of the same region of a fiducial mark is acquired at a later time. The first and second fiducial mark images are compared in order to determine whether they are images of the same fiducial mark of the same specimen slide, thereby allowing the system to detect when one specimen slide has been swapped with another specimen slide. The comparison may also involve images of multiple features on the specimen carrier, e.g., sets of multiple fiducial marks. | 01-28-2010 |
20100128944 | CYTOLOGICAL IMAGING SYSTEMS AND METHODS - The present invention relates to the analysis of specimens. Specifically, the invention relates to methods and apparatus for reviewing specimen slides, including apparatus for holding the slides. The invention also relates to an automatic focusing method for an imaging system and methods for accommodating vibration in the imaging system. In particular, the methods and apparatus may be applied to the automated analysis of cytological specimen slides. | 05-27-2010 |
20100284602 | METHOD FOR DETERMINING A COMPLETE BLOOD COUNT ON A WHITE BLOOD CELL DIFFERENTIAL COUNT - Systems and methods analyzing body fluids such as blood and bone marrow are disclosed. The systems and methods may utilize an improved technique for applying a monolayer of cells to a slide to generate a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and methods for utilizing multi color microscopy for improving the quality of images captured by a light receiving device. | 11-11-2010 |
20110014645 | METHOD FOR DETERMINING A COMPLETE BLOOD COUNT ON A WHITE BLOOD CELL DIFFERENTIAL COUNT - Systems and methods analyzing body fluids such as blood and bone marrow are disclosed. The systems and methods may utilize an improved technique for applying a monolayer of cells to a slide to generate a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and methods for utilizing multi color microscopy for improving the quality of images captured by a light receiving device. | 01-20-2011 |
20110070606 | SYSTEMS AND METHODS FOR ANALYZING BODY FLUIDS - Systems and methods analyzing body fluids contain cells including blood, bone marrow, urine, vaginal tissue, epithelial tissue, tumors, semen, and spittle are disclosed. The systems and methods utilize an improved technique for applying a monolayer of cells to a slide and generating a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and method for utilizing multi-color microscopy for improving the quality of images captured by a light receiving device. | 03-24-2011 |
20120149050 | AUTOMATED SYSTEMS AND METHODS FOR PREPARING BIOLOGICAL SPECIMENS FOR EXAMINATION - The systems and methods disclosed herein permit automated preparation of biological specimens for examination. The disclosed systems and methods provide fast, efficient, and highly uniform specimen processing using minimal quantities of fluids. The methods include at least a fixing phase for fixing a biological specimen to a substrate such as a microscope slide, a staining phase for staining the specimen, and a rinsing phase for rinsing the specimen. One or more of the fixing, staining, and rinsing phases include one or more agitation cycles for distributing reagents evenly and uniformly across the specimen. The systems can be implemented as a standalone device or as a component in a larger system for preparing and examining biological specimens. | 06-14-2012 |
20130002847 | SYSTEMS AND METHODS FOR SAMPLE DISPLAY AND REVIEW - Methods and systems for displaying images of cells in a sample include obtaining a plurality of images of cells in the sample, where each image corresponds to one of the cells in the sample, determining values of at least one property for each of the cells based on the plurality of images, arranging the plurality of images to form a first image array, where the images are ordered in the first image array based on the values of the at least one property, displaying the first image array, sorting the plurality of images to form a second image array in which an ordering of the images is different from the first image array, and displaying the second image array, where the sample includes blood and the cells include red blood cells. | 01-03-2013 |
20130070077 | Systems and Methods for Analyzing Body Fluids - Systems and methods analyzing body fluids contain cells including blood, bone marrow, urine, vaginal tissue, epithelial tissue, tumors, semen, and spittle are disclosed. The systems and methods utilize an improved technique for applying a monolayer of cells to a slide and generating a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and method for utilizing multi-color microscopy for improving the quality of images captured by a light receiving device. | 03-21-2013 |
20140004561 | AUTOMATED SYSTEMS AND METHODS FOR PREPARING BIOLOGICAL SPECIMENS FOR EXAMINATION | 01-02-2014 |
20140016841 | CONTROLLED DISPENSING OF SAMPLES ONTO SUBSTRATES - Methods for dispensing a fluid sample on a substrate include obtaining an image of a sample applicator in proximity to the substrate, where the image includes a first image of the sample applicator and a second image of the sample applicator, determining a height of the sample applicator relative to a surface plane of the substrate based on a distance between common portions of the first and second images, and dispensing the fluid sample onto the substrate using the sample applicator, where the dispensing includes: translating the sample applicator, translating the substrate, or translating both the sample applicator and the substrate to effect a relative translation between the sample applicator and the substrate; and maintaining the sample applicator within 2 microns of a target height relative to the surface plane of the substrate during the translating. | 01-16-2014 |
Patent application number | Description | Published |
20080278707 | CYTOLOGICAL IMAGING SYSTEM AND METHOD - The present invention relates to the analysis of cytological material. Specifically, the invention relates to stains and methods of producing the stains, methods of staining cells for cytological or histological analysis to contrast the nuclear portion of the cell from the cytoplasmic portion, and systems and methods for illuminating a cytological sample. The analysis can be automated or manual. | 11-13-2008 |
20130021461 | SAMPLE APPLICATOR SENSING AND POSITIONING - Systems and methods for positioning a sample applicator relative to a substrate include: (a) obtaining an image of the sample applicator in proximity to the substrate, where the image includes a direct image region corresponding to the sample applicator and a first reflected image region corresponding to an image of the sample applicator reflected from a surface of the substrate; (b) determining a position of an edge of the sample applicator in the direct image region; (c) determining a position of a reflected edge of the sample applicator in the first reflected image region; (d) determining a distance between the edge of the sample applicator and the reflected edge of the sample applicator; and (e) determining the position of the sample applicator relative to the substrate based on the distance between the edges. | 01-24-2013 |
20150262355 | METHOD AND SYSTEM FOR PROCESSING AN IMAGE OF A BIOLOGICAL SPECIMEN - Components, e.g., background, cytoplasm, nucleus and nucleolus, of a biological specimen are identified using multi-wavelength analysis. Specimen components, such as nucleoli, are selected, and a determination is made whether cells having nucleoli are cancer cells or regular repair cells based on one or more physical characteristics of the identified component. The physical characteristics can be one or more of a shape, size, texture and gray value. | 09-17-2015 |
Patent application number | Description | Published |
20120183198 | MICROSCOPE SLIDE COORDINATE SYSTEM REGISTRATION - Systems, methods and computer program products for mapping coordinates of various imaging stations are described. In some implementations, cells (e.g., red blood cells) in a biological specimen can be used for determining the mapping information between the imaging stations. The use of cells allows a target image (e.g., an image of a sub-region of cells in the biological specimen) taken by one imaging station to be pattern-matched to a reference image (e.g., an image showing a larger region of cells in the biological specimen that also includes the sub-region) taken by another imaging station. Once the target image is matched to the reference image, point by point correspondence (and therefore coordinates) between the target image and the reference image can be established for computing the coordinate transformation to map the imaging stations. | 07-19-2012 |
20120194729 | Fast Auto-Focus in Imaging - The disclosure relates to methods and systems for automatically focusing multiple images of one or more objects on a substrate. The methods include obtaining, by a processor, a representative focal distance for a first location on the substrate based on a set of focal distances at known locations on the substrate. The methods also include acquiring, by an image acquisition device, a set of at least two images of the first location. The images are each acquired using a different focal distance at an offset from the representative focal distance. The methods further include estimating, by a processor, an ideal focal distance corresponding to the first location based on comparing a quality of focus for each of the images, and storing the estimated ideal focal distance and the first location in the set of focal distances at known locations. | 08-02-2012 |
Patent application number | Description | Published |
20120262703 | Measuring Volume and Constituents of Cells - A method of determining a volume of a platelet includes: (a) illuminating the platelet with incident light at a plurality of illumination wavelengths; (b) obtaining at least one two-dimensional image of the platelet corresponding to each illumination wavelength; (c) for each illumination wavelength, determining a mean optical density and a maximum optical density for the platelet; (d) determining an area of the platelet; (e) for each illumination wavelength, determining a volume of the platelet; (f) for each illumination wavelength, determining an integrated optical density for the platelet; and (g) determining the volume of the platelet based on a weighted combination of the area of the platelet, the volumes of the platelet corresponding to each of the illumination wavelengths, and the integrated optical densities for the platelet corresponding to each of the illumination wavelengths. | 10-18-2012 |
20120262704 | Measuring Volume and Constituents of Cells - A method for determining a mean cell volume for a blood sample includes: illuminating the sample with incident light at a plurality of illumination wavelengths and obtaining a two-dimensional image of the sample at each of the plurality of illumination wavelengths; identifying a plurality of cells that appear in each of the images; for each one of the plurality of cells, determining an integrated optical density corresponding to each of the plurality of illumination wavelengths; for each one of the plurality of cells, determining a cell volume based on the integrated optical densities corresponding to each of the plurality of illumination wavelengths; and determining the mean cell volume for the blood sample from the cell volumes for each one of the plurality of cells. | 10-18-2012 |
20120262705 | Measuring Volume and Constituents of Cells - A method of determining a hemoglobin content value of a red blood cell includes: (a) illuminating the cell with incident light at a plurality of illumination wavelengths; (b) obtaining at least one two-dimensional image of the cell corresponding to each illumination wavelength; (c) for each illumination wavelength, determining a mean optical density and a maximum optical density for the cell; (d) determining an area of the cell; (e) for each illumination wavelength, determining a volume of the cell; (f) for each illumination wavelength, determining an integrated optical density for the cell; and (g) determining the hemoglobin content value of the cell based on the area of the cell, the volumes of the cell corresponding to each of the illumination wavelengths, and the integrated optical densities for the cell corresponding to each of the illumination wavelengths. | 10-18-2012 |
20130002847 | SYSTEMS AND METHODS FOR SAMPLE DISPLAY AND REVIEW - Methods and systems for displaying images of cells in a sample include obtaining a plurality of images of cells in the sample, where each image corresponds to one of the cells in the sample, determining values of at least one property for each of the cells based on the plurality of images, arranging the plurality of images to form a first image array, where the images are ordered in the first image array based on the values of the at least one property, displaying the first image array, sorting the plurality of images to form a second image array in which an ordering of the images is different from the first image array, and displaying the second image array, where the sample includes blood and the cells include red blood cells. | 01-03-2013 |
20130021461 | SAMPLE APPLICATOR SENSING AND POSITIONING - Systems and methods for positioning a sample applicator relative to a substrate include: (a) obtaining an image of the sample applicator in proximity to the substrate, where the image includes a direct image region corresponding to the sample applicator and a first reflected image region corresponding to an image of the sample applicator reflected from a surface of the substrate; (b) determining a position of an edge of the sample applicator in the direct image region; (c) determining a position of a reflected edge of the sample applicator in the first reflected image region; (d) determining a distance between the edge of the sample applicator and the reflected edge of the sample applicator; and (e) determining the position of the sample applicator relative to the substrate based on the distance between the edges. | 01-24-2013 |
20130023007 | IDENTIFYING AND MEASURING RETICULOCYTES - Methods and systems for identifying reticulocytes in a blood sample deposited on a substrate include: illuminating the sample with incident light at two different wavelengths, obtaining a two-dimensional image of the sample corresponding to a first one of the wavelengths, and obtaining a two-dimensional image of the sample corresponding to a second one of the wavelengths; analyzing the images to identify a set of representative red blood cells; determining an area of each of the red blood cells in the set; determining a color value of each of the red blood cells in the set; and, for each one of the red blood cells in the set, identifying the red blood cell as a reticulocyte if the area of the red blood cell exceeds an area cutoff value and the color value of the red blood cell is less than a color cutoff value. | 01-24-2013 |
20130077085 | Measuring Volume and Constituents of Cells - A method of determining a volume of a platelet includes: (a) illuminating the platelet with incident light at a plurality of illumination wavelengths; (b) obtaining at least one two-dimensional image of the platelet corresponding to each illumination wavelength; (c) for each illumination wavelength, determining a mean optical density and a maximum optical density for the platelet; (d) determining an area of the platelet; (e) for each illumination wavelength, determining a volume of the platelet; (f) for each illumination wavelength, determining an integrated optical density for the platelet; and (g) determining the volume of the platelet based on a weighted combination of the area of the platelet, the volumes of the platelet corresponding to each of the illumination wavelengths, and the integrated optical densities for the platelet corresponding to each of the illumination wavelengths. | 03-28-2013 |
20130279788 | Measuring Volume and Constituents of Cells - A method for determining a mean cell volume for a blood sample includes: illuminating the sample with incident light at a plurality of illumination wavelengths and obtaining a two-dimensional image of the sample at each of the plurality of illumination wavelengths; identifying a plurality of cells that appear in each of the images; for each one of the plurality of cells, determining an integrated optical density corresponding to each of the plurality of illumination wavelengths; for each one of the plurality of cells, determining a cell volume based on the integrated optical densities corresponding to each of the plurality of illumination wavelengths; and determining the mean cell volume for the blood sample from the cell volumes for each one of the plurality of cells. | 10-24-2013 |
20140016841 | CONTROLLED DISPENSING OF SAMPLES ONTO SUBSTRATES - Methods for dispensing a fluid sample on a substrate include obtaining an image of a sample applicator in proximity to the substrate, where the image includes a first image of the sample applicator and a second image of the sample applicator, determining a height of the sample applicator relative to a surface plane of the substrate based on a distance between common portions of the first and second images, and dispensing the fluid sample onto the substrate using the sample applicator, where the dispensing includes: translating the sample applicator, translating the substrate, or translating both the sample applicator and the substrate to effect a relative translation between the sample applicator and the substrate; and maintaining the sample applicator within 2 microns of a target height relative to the surface plane of the substrate during the translating. | 01-16-2014 |
20150092200 | Measuring Volume and Constituents of Cells - A method for determining a mean cell volume for a blood sample includes: illuminating the sample with incident light at a plurality of illumination wavelengths and obtaining a two-dimensional image of the sample at each of the plurality of illumination wavelengths; identifying a plurality of cells that appear in each of the images; for each one of the plurality of cells, determining an integrated optical density corresponding to each of the plurality of illumination wavelengths; for each one of the plurality of cells, determining a cell volume based on the integrated optical densities corresponding to each of the plurality of illumination wavelengths; and determining the mean cell volume for the blood sample from the cell volumes for each one of the plurality of cells. | 04-02-2015 |
20150256739 | Fast Auto-Focus in Imaging - The disclosure relates to methods and systems for automatically focusing multiple images of one or more objects on a substrate. The methods include obtaining, by a processor, a representative focal distance for a first location on the substrate based on a set of focal distances at known locations on the substrate. The methods also include acquiring, by an image acquisition device, a set of at least two images of the first location. The images are each acquired using a different focal distance at an offset from the representative focal distance. The methods further include estimating, by a processor, an ideal focal distance corresponding to the first location based on comparing a quality of focus for each of the images, and storing the estimated ideal focal distance and the first location in the set of focal distances at known locations. | 09-10-2015 |
20150324628 | MICROSCOPE SLIDE COORDINATE SYSTEM REGISTRATION - Systems, methods and computer program products for mapping coordinates of various imaging stations are described. In some implementations, cells (e.g., red blood cells) in a biological specimen can be used for determining the mapping information between the imaging stations. The use of cells allows a target image (e.g., an image of a sub-region of cells in the biological specimen) taken by one imaging station to be pattern-matched to a reference image (e.g., an image showing a larger region of cells in the biological specimen that also includes the sub-region) taken by another imaging station. Once the target image is matched to the reference image, point by point correspondence (and therefore coordinates) between the target image and the reference image can be established for computing the coordinate transformation to map the imaging stations. | 11-12-2015 |
Patent application number | Description | Published |
20120183198 | MICROSCOPE SLIDE COORDINATE SYSTEM REGISTRATION - Systems, methods and computer program products for mapping coordinates of various imaging stations are described. In some implementations, cells (e.g., red blood cells) in a biological specimen can be used for determining the mapping information between the imaging stations. The use of cells allows a target image (e.g., an image of a sub-region of cells in the biological specimen) taken by one imaging station to be pattern-matched to a reference image (e.g., an image showing a larger region of cells in the biological specimen that also includes the sub-region) taken by another imaging station. Once the target image is matched to the reference image, point by point correspondence (and therefore coordinates) between the target image and the reference image can be established for computing the coordinate transformation to map the imaging stations. | 07-19-2012 |
20120262703 | Measuring Volume and Constituents of Cells - A method of determining a volume of a platelet includes: (a) illuminating the platelet with incident light at a plurality of illumination wavelengths; (b) obtaining at least one two-dimensional image of the platelet corresponding to each illumination wavelength; (c) for each illumination wavelength, determining a mean optical density and a maximum optical density for the platelet; (d) determining an area of the platelet; (e) for each illumination wavelength, determining a volume of the platelet; (f) for each illumination wavelength, determining an integrated optical density for the platelet; and (g) determining the volume of the platelet based on a weighted combination of the area of the platelet, the volumes of the platelet corresponding to each of the illumination wavelengths, and the integrated optical densities for the platelet corresponding to each of the illumination wavelengths. | 10-18-2012 |
20120262704 | Measuring Volume and Constituents of Cells - A method for determining a mean cell volume for a blood sample includes: illuminating the sample with incident light at a plurality of illumination wavelengths and obtaining a two-dimensional image of the sample at each of the plurality of illumination wavelengths; identifying a plurality of cells that appear in each of the images; for each one of the plurality of cells, determining an integrated optical density corresponding to each of the plurality of illumination wavelengths; for each one of the plurality of cells, determining a cell volume based on the integrated optical densities corresponding to each of the plurality of illumination wavelengths; and determining the mean cell volume for the blood sample from the cell volumes for each one of the plurality of cells. | 10-18-2012 |
20120262705 | Measuring Volume and Constituents of Cells - A method of determining a hemoglobin content value of a red blood cell includes: (a) illuminating the cell with incident light at a plurality of illumination wavelengths; (b) obtaining at least one two-dimensional image of the cell corresponding to each illumination wavelength; (c) for each illumination wavelength, determining a mean optical density and a maximum optical density for the cell; (d) determining an area of the cell; (e) for each illumination wavelength, determining a volume of the cell; (f) for each illumination wavelength, determining an integrated optical density for the cell; and (g) determining the hemoglobin content value of the cell based on the area of the cell, the volumes of the cell corresponding to each of the illumination wavelengths, and the integrated optical densities for the cell corresponding to each of the illumination wavelengths. | 10-18-2012 |
20130021461 | SAMPLE APPLICATOR SENSING AND POSITIONING - Systems and methods for positioning a sample applicator relative to a substrate include: (a) obtaining an image of the sample applicator in proximity to the substrate, where the image includes a direct image region corresponding to the sample applicator and a first reflected image region corresponding to an image of the sample applicator reflected from a surface of the substrate; (b) determining a position of an edge of the sample applicator in the direct image region; (c) determining a position of a reflected edge of the sample applicator in the first reflected image region; (d) determining a distance between the edge of the sample applicator and the reflected edge of the sample applicator; and (e) determining the position of the sample applicator relative to the substrate based on the distance between the edges. | 01-24-2013 |
20130023007 | IDENTIFYING AND MEASURING RETICULOCYTES - Methods and systems for identifying reticulocytes in a blood sample deposited on a substrate include: illuminating the sample with incident light at two different wavelengths, obtaining a two-dimensional image of the sample corresponding to a first one of the wavelengths, and obtaining a two-dimensional image of the sample corresponding to a second one of the wavelengths; analyzing the images to identify a set of representative red blood cells; determining an area of each of the red blood cells in the set; determining a color value of each of the red blood cells in the set; and, for each one of the red blood cells in the set, identifying the red blood cell as a reticulocyte if the area of the red blood cell exceeds an area cutoff value and the color value of the red blood cell is less than a color cutoff value. | 01-24-2013 |
20130024130 | BLOOD ANALYZER CALIBRATION AND ASSESSMENT - Systems and methods for displaying measured values of a complete blood count (“CBC”) parameter include displaying the measured values of the CBC parameter obtained from a plurality of samples from a first lot of a quality control composition, where the displaying includes displaying a marker corresponding to each measured value from the first lot on a plot that includes a two dimensional coordinate system, and where the two dimensional coordinate system includes a first dimension corresponding to a time at which measured values of the CBC parameter were obtained, and a second dimension corresponding to a numerical value of the CBC parameter. | 01-24-2013 |
20130077085 | Measuring Volume and Constituents of Cells - A method of determining a volume of a platelet includes: (a) illuminating the platelet with incident light at a plurality of illumination wavelengths; (b) obtaining at least one two-dimensional image of the platelet corresponding to each illumination wavelength; (c) for each illumination wavelength, determining a mean optical density and a maximum optical density for the platelet; (d) determining an area of the platelet; (e) for each illumination wavelength, determining a volume of the platelet; (f) for each illumination wavelength, determining an integrated optical density for the platelet; and (g) determining the volume of the platelet based on a weighted combination of the area of the platelet, the volumes of the platelet corresponding to each of the illumination wavelengths, and the integrated optical densities for the platelet corresponding to each of the illumination wavelengths. | 03-28-2013 |
20130279788 | Measuring Volume and Constituents of Cells - A method for determining a mean cell volume for a blood sample includes: illuminating the sample with incident light at a plurality of illumination wavelengths and obtaining a two-dimensional image of the sample at each of the plurality of illumination wavelengths; identifying a plurality of cells that appear in each of the images; for each one of the plurality of cells, determining an integrated optical density corresponding to each of the plurality of illumination wavelengths; for each one of the plurality of cells, determining a cell volume based on the integrated optical densities corresponding to each of the plurality of illumination wavelengths; and determining the mean cell volume for the blood sample from the cell volumes for each one of the plurality of cells. | 10-24-2013 |
20140016841 | CONTROLLED DISPENSING OF SAMPLES ONTO SUBSTRATES - Methods for dispensing a fluid sample on a substrate include obtaining an image of a sample applicator in proximity to the substrate, where the image includes a first image of the sample applicator and a second image of the sample applicator, determining a height of the sample applicator relative to a surface plane of the substrate based on a distance between common portions of the first and second images, and dispensing the fluid sample onto the substrate using the sample applicator, where the dispensing includes: translating the sample applicator, translating the substrate, or translating both the sample applicator and the substrate to effect a relative translation between the sample applicator and the substrate; and maintaining the sample applicator within 2 microns of a target height relative to the surface plane of the substrate during the translating. | 01-16-2014 |
20150092200 | Measuring Volume and Constituents of Cells - A method for determining a mean cell volume for a blood sample includes: illuminating the sample with incident light at a plurality of illumination wavelengths and obtaining a two-dimensional image of the sample at each of the plurality of illumination wavelengths; identifying a plurality of cells that appear in each of the images; for each one of the plurality of cells, determining an integrated optical density corresponding to each of the plurality of illumination wavelengths; for each one of the plurality of cells, determining a cell volume based on the integrated optical densities corresponding to each of the plurality of illumination wavelengths; and determining the mean cell volume for the blood sample from the cell volumes for each one of the plurality of cells. | 04-02-2015 |