Patent application number | Description | Published |
20100273984 | METHOD FOR ENRICHING PHOSPHOPEPTIDES - The invention relates to a method for enriching phosphopeptides. Said method is characterized in that a carrier is used which carries phosphate groups and/or phosphonate groups on the surface thereof. The phosphate groups and/or phosphonate groups are functionalized with zirconium ions and are bonded to the carrier by means of linker structures which have at least one alkyl chain containing at least 5 C atoms. Also disclosed are corresponding carriers and suitable kits for enriching phosphopeptides. | 10-28-2010 |
20120077969 | NUCLEIC ACID PURIFICATION METHOD - The present invention relates to a method for purifying a defined amount of nucleic acids from a nucleic acid-containing sample, which has at least the following steps: (a.) contacting the nucleic acid-containing sample with a defined amount of a nucleic acid binding phase with the following features: (i) the nucleic acid binding phase has nucleic acid binding ligands that have at least one protonatable group; (ii) the nucleic acid binding ligands are bound to a carrier; (iii) the nucleic acid binding phase has a surface with a low charge density, wherein the amount of nucleic acids in the sample exceeds the binding capacity of the amount of nucleic acid binding phase used; (b.) binding of the nucleic acids to the nucleic acid binding phase at a pH (binding pH) that is below the pKs value of at least one of the protonatable groups; (c.) elution of the nucleic acids at a pH that is above the binding pH, wherein a defined amount of nucleic acids is obtained. Furthermore, corresponding kits and nucleic acid binding phases, which can be used for the purification of nucleic acids, are disclosed. | 03-29-2012 |
20120245337 | NUCLEIC ACID PURIFICATION METHOD - The present intimation relates to a method for purifying nucleic acids from a sample containing nucleic acids, the method comprising at least the following steps: a. bringing the sample containing nucleic acids into contact with a nucleic acid binding phase comprising protonatable groups, wherein the protonatable groups have a pKa value of 9 to 12; b. binding the nucleic acids to the nucleic acid phase at a pH (binding pH) that is at least one pH unit less than the pKa value of at least one of the protonatable groups; c. eluting the nucleic acids at a pH greater than the binding pH but at least one pH unit less than the pKa value of at least one of the protonatable groups. Also disclosed are corresponding kits and nucleic acid binding phases that can be used for purifying nucleic acids. | 09-27-2012 |
Patent application number | Description | Published |
20120181841 | SEAT CUSHION BODY AND METHOD OF PRODUCING A SEAT CUSHION BODY - A seat cushion body and method of producing the same are provided. In a method of producing a seat cushion body, fiber material is inserted into a mold. Heat is supplied to the fibers to cause thermal activation of binding fibers, so as to effect thermal cross-linking. The resulting seat cushion body includes a resilient portion formed from the fibers and having a fiber orientation along a main load direction of the seat cushion body. The seat cushion body may include at least one further portion formed from the fibers, which has a fiber orientation and/or a density different from the fiber orientation and/or different from a density of the resilient portion. The resilient portion and the at least one further portion may be integrally formed from the cross-linked fiber material as a unitary body. | 07-19-2012 |
20140167328 | METHOD OF TREATING A FIBER CUSHION BODY - A fiber cushion body, which is formed from cross-linked fiber material and which is resiliently deformable when load is applied along a main load direction, is treated using ultrasonic energy. A portion of the fiber cushion body is displaced, for example using a sonotrode, and ultrasonic vibrations are applied to the fiber cushion body to re-shape the fiber cushion body from a first shape to a second shape different from the first shape. | 06-19-2014 |
20140354029 | SEAT CUSHION BODY AND METHOD OF PRODUCING A SEAT CUSHION BODY - A seat cushion body and method of producing the same are provided. In a method of producing a seat cushion body, fiber material is inserted into a mold. Heat is supplied to the fibers to cause thermal activation of binding fibers, so as to effect thermal cross-linking. The resulting seat cushion body includes a resilient portion formed from the fibers and having a fiber orientation along a main load direction of the seat cushion body. The seat cushion body may include at least one further portion formed from the fibers, which has a fiber orientation and/or a density different from the fiber orientation and/or different from a density of the resilient portion. The resilient portion and the at least one further portion may be integrally formed from the cross-linked fiber material as a unitary body. | 12-04-2014 |