Patent application number | Description | Published |
20090286247 | Novel nucleic acid base pair - A novel artificial nucleic acid base pair which is obtained by forming a selective base pair by introducing a group having steric hindrance (preferably a group having steric hindrance and static repulsion and a stacking effect) and can be recognized by a polymerase such as DNA polymerase; a novel artificial gene; and a method of designing nucleic acid bases so as to form a selective base pair with the use of steric hindrance, static repulsion and stacking effect at the base moiety of the nucleic acid. An artificial nucleic acid comprising these bases; a process for producing the same; a codon containing the same; a nucleic acid molecule containing the same; a process for producing a non-natural gene by using the same; a process for producing a novel protein by using the above nucleic acid molecule or non-natural gene, and the like. | 11-19-2009 |
20090286311 | DNA sequences for the expression of alloproteins - The present invention discloses an expression method for non-naturally-occurring amino acid-containing protein comprising: expressing in animal cells: (A) a mutant tyrosyl-tRNA synthetase that is a mutation of tyrosyl-tRNA synthetase originating in | 11-19-2009 |
20100155799 | SEMICONDUCTOR DEVICE AND METHOD FOR MANUFACTURING THE SAME - A first MOS transistor includes, as a first impurity region, a pair of first source/drain regions including first portions formed in a semiconductor substrate and second portions formed so as to project upward from the first portions. A second MOS transistor includes a pair of second source/drain regions including second impurity regions formed in the semiconductor substrate, third impurity regions located in contact with the second impurity regions so as to project upward from the semiconductor substrate, and fourth impurity regions located on the third impurity regions. The concentration of impurities in the third impurity regions is lower than that of impurities in the fourth impurity regions. The concentration of impurities in the first impurity regions is lower than that of impurities in the second impurity regions. The first, the second, the third and the fourth impurity regions are same conductivity type. | 06-24-2010 |
20100304431 | tRNA SYNTHESIS METHOD, NUCLEIC ACID, AMINOACYL tRNA SYNTHESIS METHOD, AND PROCESS FOR PRODUCTION OF PROTEIN INTRODUCED WITH UNNATURAL AMINO ACID - The present invention relates to a process for producing a protein having an unnatural amino acid introduced therein, the process including: expressing in a eukaryotic cell an aminoacyl-tRNA synthetase, a nucleic acid having a sequence containing a eukaryote-derived tRNA nucleotide sequence linked to the 5′ end of a tRNA nucleotide sequence that is ligated with to an unnatural amino acid in the presence of the aminoacyl-tRNA synthetase, an unnatural amino acid, and a gene of a desired protein having a nonsense mutation at a predetermined position, to integrate the unnatural amino acid at the nonsense mutation position into the protein, thereby expressing a protein having an unnatural amino acid introduced therein. | 12-02-2010 |
20130102776 | ANTICANCER AGENT - An anticancer agent comprising a compound represented by the formula (I) [R | 04-25-2013 |
Patent application number | Description | Published |
20080206820 | Labeling Methods with Oxygen Isotopes - The present invention provides methods for labeling One or two oxygen atom(s) in a carboxyl group of a carboxyl-containing compound with an oxygen isotope selected from oxygen-17 ( | 08-28-2008 |
20090233290 | Mutant SepRS, and method for site-specific introduction of phosphoserine into protein using the same - A mutant SepRS which is suitable for a site-specific introduction of phosphoserine into a protein is prepared by analyzing the structure and functions of a phosphoseryl-tRNA synthetase (SepRS) derived from an archaebacterium. A mutant SepRS composed of an amino acid sequence depicted in SEQ ID NO:2, in which any one or more of glutamic acids at position-418 and position-420 and threonine at position-423 are substituted with other amino acid, and having enhanced binding affinity with a suppressor tRNA as compared with a wild type phosphoseryl-tRNA synthetase (SepRS) composed of an amino acid sequence depicted in SEQ ID NO:2 is provided. | 09-17-2009 |
20090275017 | Novel Nucleoside or Nucleotide Derivative and Use Thereof - The object of the present invention is to provide a nucleoside or nucleotide having a 5-substituted-2-oxo(1H)pyridin-3-yl group as a base, as well as a method using the same. | 11-05-2009 |
20100267087 | MUTANT PYRROLYSYL-tRNA SYNTHETASE, AND METHOD FOR PRODUCTION OF PROTEIN HAVING NON-NATURAL AMINO ACID INTEGRATED THEREIN BY USING THE SAME - Method for incorporating a lysine derivative (particularly an N | 10-21-2010 |
20140194320 | METHOD FOR PREPARING NUCLEIC ACID APTAMER - An object of the present invention is to develop and provide a method for efficiently and conveniently producing a nucleic acid aptamer, particularly, a DNA aptamer, having high specificity for and high binding activity against a target substance. The present invention provides a method for producing a nucleic acid aptamer, comprising: a complex formation step of mixing a single-stranded nucleic acid library with a target substance in a solution to form a complex of a single-stranded nucleic acid and the target substance; an immobilization step of mixing the solution after the preceding step with a solid-phase support to immobilize the complex onto the solid-phase support via connector(s) adsorbed on the target substance and/or the solid-phase support; a recovery step of recovering the complex immobilized on the solid-phase support from the solution; an amplification step of recovering the single-stranded nucleic acid from the complex, followed by amplification by a nucleic acid amplification method; and a single-stranded nucleic acid preparation step of converting the double-stranded nucleic acids obtained in the amplification step into single strands and then forming an intramolecular conformation. | 07-10-2014 |