Patent application number | Description | Published |
20090275086 | ASSEMBLY OF LARGE NUCLEIC ACIDS - A method to assemble any desired nucleic acid molecule by combining cassettes in vitro to form assemblies which are further combined in vivo, or by assembling large numbers of DNA fragments by recombination in a yeast culture to obtain desired DNA molecules of substantial size is described. | 11-05-2009 |
20100035768 | METHODS FOR IN VITRO JOINING AND COMBINATORIAL ASSEMBLY OF NUCLEIC ACID MOLECULES - The present invention relates to methods of joining two or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule of each pair share a region of sequence identity. The method allows the joining of a large number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. Kits for performing the method are also disclosed. The methods of joining DNA molecules may be used to generate combinatorial libraries useful to generate, for example, optimal protein expression through codon optimization, gene optimization, and pathway optimization. | 02-11-2010 |
20110053272 | METHODS FOR CLONING AND MANIPULATING GENOMES - Compositions and methods are disclosed herein for cloning a donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells. | 03-03-2011 |
20110053273 | METHODS FOR CLONING AND MANIPULATING GENOMES - Compositions and methods are disclosed herein for cloning a synthetic or a semi-synthetic donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells. | 03-03-2011 |
20130225451 | MATERIALS AND METHODS FOR THE SYNTHESIS OF ERROR-MINIMIZED NUCLEIC ACID MOLECULES - The present invention provides materials and methods useful for error correction of nucleic acid molecules. In one embodiment of the invention, a first plurality of double-stranded nucleic acid molecules having a nucleotide mismatch are fragmented by exposure to a molecule having unidirectional mismatch endonuclease activity. The nucleic acid molecules are cut at the mismatch site or near the mismatch site, leaving a double-stranded nucleic acid molecule having a mismatch at the end or near end of the molecule. The nucleic acid molecule is then exposed to a molecule having unidirectional exonuclease activity to remove the mismatched nucleotide. The missing nucleotides can then be filled in by the action of, e.g., a molecule having DNA polymerase activity. The result is double-stranded nucleic acid molecules with a decreased frequency of nucleotide mismatches. Also provided are novel nucleic acid sequences encoding mismatch endonucleases, polypeptides encoded thereby, as well as nucleic acid constructs, transgenic cells, and various compositions thereof. | 08-29-2013 |
Patent application number | Description | Published |
20100184187 | IN VITRO RECOMBINATION METHOD - The present invention relates, e.g., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising contacting the two DNA molecules in a reaction mixture with (a) a non-processive 5′ exonuclease; (b) a single stranded DNA binding protein (SSB) which accelerates nucleic acid annealing; (c) a non strand-displacing DNA polymerase; and (d) a ligase, under conditions effective to join the two DNA molecules to form an intact double stranded DNA molecule, in which a single copy of the region of sequence identity is retained. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. | 07-22-2010 |
20100311126 | METHOD FOR IN VITRO RECOMBINATION - The present invention relates, e.g., to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising (a) chewing back the DNA molecules with an enzyme having an exonuclease activity, to yield single-stranded overhanging portions of each DNA molecule which contain a sufficient length of the region of sequence identity to hybridize specifically to each other; (b) specifically annealing the single-stranded overhangs; and (c) repairing single-stranded gaps in the annealed DNA molecules and sealing the nicks thus formed (ligating the nicked DNA molecules). The region of sequence identity generally comprises at least 20 non-palindromic nucleotides (nt), e.g., at least about 40 non-palindromic nt. In some embodiments of the invention, about 5% PEG is present during all steps of the reaction, and/or the repair reaction is achieved with Taq DNA polymerase and a compatible ligase, such as Taq DNA ligase. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. | 12-09-2010 |
20130295645 | Method for in vitro Recombination - The present invention relates to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. | 11-07-2013 |
Patent application number | Description | Published |
20110002585 | FIBER-BASED MID-IR SIGNAL COMBINER AND METHOD OF MAKING SAME - The present invention is generally directed to a device comprising multiple specialty glass optical fibers that combines several different mid-infrared optical signals from multiple optical fibers into one signal in a single optical fiber. In addition, the present invention provides for a method of making the device. | 01-06-2011 |
20120141079 | PHOTONIC BAND GAP FIBERS USING A JACKET WITH A DEPRESSED SOFTENING TEMPERATURE - The present invention is generally directed to a photonic bad gap fiber and/or fiber preform with a central structured region comprising a first non-silica based glass and a jacket comprising a second non-silica based glass surrounding the central structured region, where the Littleton softening temperature of the second glass is at least one but no more than ten degrees Celsius lower than the Littleton softening temperature of the first glass, or where the base ten logarithm of the glass viscosity in poise of the second glass is at least 0.01 but no more than 2 lower than the base ten logarithm of the glass viscosity in poise of the first glass at a fiber draw temperature. Also disclosed is a method of making a photonic bad gap fiber and/or fiber preform. | 06-07-2012 |
20120141080 | HEXAGONAL TUBE STACKING METHOD FOR THE FABRICATION OF HOLLOW CORE PHOTONIC BAND GAP FIBERS AND PREFORMS - The present invention is generally directed to a method of making a hollow-core photonic band gap preform from a specialty glass by pressing a specialty glass through a die to form a tube wherein the outer transverse shape of the tube is a hexagon, triangle, quadrilateral, or other polygon; stretching the tube to form a micro-tube with approximately the same outer transverse shape as the tube; stacking a plurality of micro-tubes into a bundle minimizing voids between adjacent micro-tubes and forming a central longitudinal void wherein the plurality of micro-tubes within the bundle comprise an inner structured region of the preform and the central void of the bundle comprises a hollow core in the preform; and inserting the bundle into a jacket tube. Also disclosed are the hollow-core photonic band gap preform and fiber formed by this method. | 06-07-2012 |
20120206796 | INFRARED (IR) TRANSMITTING GRADED INDEX (GRIN) OPTICAL ELEMENTS AND METHOD FOR MAKING SAME - The present invention is directed to a method for making infrared transmitting graded index optical elements by selecting at least two different infrared-transmitting materials, each with a different refractive index, having similar thermo-viscous behavior; assembling the infrared-transmitting materials into a stack comprising one or more layers of each infrared-transmitting material resulting in the stack having a graded index profile; and forming the stack into a desired shape. Also disclosed is the related optical element made by this method. | 08-16-2012 |
20120321263 | DIRECT EXTRUSION METHOD FOR THE FABRICATION OF PHOTONIC BAND GAP (PBG) FIBERS AND FIBER PREFORMS - A method and apparatus for making a substantially void-free preform for a microstructured optical fiber using a one-step process is provided. A preform is prepared from specialty glasses using a direct extrusion method. A die for use with the direct extrusion method is also provided, and a method for drawing the preform into a HC-PBG fiber for use in transmitting infra-red wavelength light is also provided. The preform comprises an outer jacket made of solid glass, a cladding having a plurality of air holes arranged in a desired pattern within the jacket, and a core which is hollow. | 12-20-2012 |
20140003776 | Multi-Core Optical Fibers for IR Image Transmission | 01-02-2014 |
20140260693 | SYSTEM APPARATUS AND METHOD OF SAMPLING A BROAD RANGE OF CHEMICALS (SBRC) BY COLLECTION AND ANALYSIS OF CHEMICAL VAPORS AEROSOLS PARTICULATES AND LIQUID DROPLETS ON SURFACES - Sampling of a broad range of chemicals using a handheld sampler body, having a sample screen in a sampling cassette; where a sample screen housing further consists of a locking arm arrestor body, where, the locking arm arrestor body including a draw tube, and where the sampling screen can be positioned in either a retracted or extended positioned regarding a sample access face. When activated, the system executes collecting and sampling operations of chemicals, by exposing the extended sampling screen to a sampling environment, drawing through the draw tube, air from the sampling environment, further collecting, onto the surface of the sample screen solid particles and/or pressing the sample screen against the surface(s) of object in the sampling environment. Then, removing the sampling screen from the sampling environment and isolating the plurality of airborne chemical contaminants, by retracting a sample screen into the sample screen housing. | 09-18-2014 |