51st week of 2008 patent applcation highlights part 41 |
Patent application number | Title | Published |
20080311558 | Methods For Rapid Identification Of Pathogens In Humans And Animals - The present invention provides methods of: identifying pathogens in biological samples from humans and animals, resolving a plurality of etiologic agents present in samples obtained from humans and animals, determining detailed genetic information about such pathogens or etiologic agents, and rapid detection and identification of bioagents from environmental, clinical or other samples. | 2008-12-18 |
20080311559 | DETECTION OF NUCLEIC ACIDS FROM MULTIPLE TYPES OF HUMAN PAPILLOMAVIRUSES - Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed. | 2008-12-18 |
20080311560 | Oligonucleotides For Detecting Human Papilloma Virus In A Test Sample - Oligonucleotides targeted to HPV Type 16 and/or Type 18 nucleic acid sequences which are particularly useful to aid in detecting HPV type 16 and or 18 are described. The oligonucleotides can aid in detecting HPV Type 16 and/or Type 18 in different ways such as by acting as hybridization assay probes, helper probes, and/or amplification primers. | 2008-12-18 |
20080311561 | Kits and Method For Detecting Human Papilloma Virus With Oligo Nucleotide Bead Array - Provided are determining methods of human papillomavirus (HPV) genotypes with a high sensitivity. The method includes performing two-step PCRs on an HPV L1 gene in a sample to be analyzed as a biotin-labeled, single-stranded L1 gene, performing a hybridization reaction on the biotin-labeled, single-stranded L1 gene with a HPV genotype detection probe, reacting the hybridization reaction product with fluorescent substance combined with streptavidine, and measuring a fluorescent substance level to identify the HPV genotype. The detection method has high sensitivity enough to detect an extremely small amount of HPV in the sample. In addition, the high specificity exhibited by the detection method enables accurate diagnosis specific to HPV type. | 2008-12-18 |
20080311562 | Nucleic Acid Sequencing - A method for determining a target nucleic acid sequence, wherein the target nucleic acid sequence is comprised in a preparation comprising a non-target nucleic acid sequence, the target nucleic acid sequence and the non-target nucleic acid sequence each having a first region of common sequence upstream of a first region of dissimilar sequence upstream of a second region of dissimilar sequence, the method comprising: | 2008-12-18 |
20080311563 | Methods for selecting medications - Methods for selecting a medication for a patient are described that include determining the patient's genotype for a panel of genes and selecting the medication based on the genotype. Articles of manufacture also are provided that include nucleic acid molecules for detecting alleles of genes encoding drug metabolizing enzymes and genes encoding products involved in neurotransmission. | 2008-12-18 |
20080311564 | Sequences and Methods for Detection of Cytomegalovirus - Amplification primers and methods for specific amplification and detection of a CMV target are disclosed. The primer-target binding sequences are useful for amplification and detection of the CMV target in a variety of amplification and detection reactions. | 2008-12-18 |
20080311565 | Methods and Kits for Detecting Germ Cell Genomic Instability - Disclosed are methods for detecting microsatellite instability in the germ line of males, methods of assessing risk for developing testicular cancer, methods of evaluating the microsatellite stability of putative cancer or precancerous cells or a tumor, methods for evaluating germ cells for exposure to mutagens, and kits for use in the methods of the invention. | 2008-12-18 |
20080311566 | Method for Quantification of Methylated Dna - The present invention relates to a method for the quantification of methylated cytosines in DNA. In the first step of the invention unmethylated cytosines in the DNA to be analysed are chemically converted into uracil while 5-methylcytosines remain unchanged. In a second step the converted DNA is amplified methylation specifically in a real time PCR using a methylation specific probe. Finally the amount of uniformly methylated DNA is calculated by combining criteria derived from the shape of the real time curve and from the signal intensity. The method is preferably used for diagnosis and/or prognosis of adverse events for individuals, for distinguishing cell types and tissues, or for investigating cell differentiation. | 2008-12-18 |
20080311567 | Tumor Markers for Use in the Diagnosis of Colorectal Carcinomas and/or Metastases Originating Therefrom - The invention relates to a method (i) for detecting a carcinoma, especially an adenocarcinoma, preferably a gastrointestinal carcinoma and more preferably a colorectal carcinoma, (ii) for predicting metastases, preferably liver metastases, depending on a primary colon carcinoma and/or (iii) for predicting the response of metastases to a 5-fluorouracil-containing chemotherapy. The inventive method comprises determining a gene expression profile of 120 marker genes or a selection thereof. | 2008-12-18 |
20080311568 | Compositions and Methods for Nucleic Acid Analysis of Sequences with Insertions or Deletions - Contemplated methods and kits include a plurality of single stranded oligonucleotides that comprise a discriminating sequence that is encompassed on one end by a label and on the other end by a unique tag sequence. In one preferred aspect, the discriminating sequence is an RNA repeat unit, forms a heteroduplex with a complementary DNA repeat unit of the target nucleic acid, and the discriminating agent is RNaseH. Using such systems, the number of repeat units can be simply identified by hydrolysis of the discriminating sequence where such sequence is adjacent to a predetermined number of DNA repeat units in the single stranded oligonucleotide. | 2008-12-18 |
20080311569 | METHOD FOR QUANTITATIVE MEASUREMENT OF GENE EXPRESSION FOR INDENTIFYING INDIVIDUALS AT RISK FOR BRONCHOGENIC CARCINOMA - A method measure expression of multiple target genes in a progenitor cell for bronchogenic carcinoma comprising the use of reverse transcription-polymerase chain reaction (RT-PCR) to allow simultaneous expression measurement of the multiple target genes is disclosed. | 2008-12-18 |
20080311570 | CANCER SCREENING METHOD - A method for screening cancer comprises the following steps: (1) providing a test specimen; (2) detecting the methylation state of the CpG sequence in at least one target gene within the genomic DNA of the test specimen, wherein the target genes is consisted of SOX1, PAX1, LMX1A, NKX6-1, WT1 and ONECUT1; and (3) determining whether there is cancer or cancerous pathological change in the specimen based on the presence or absence of the methylation state in the target gene; wherein method for detecting methylation state is methylation-specific PCR (MSP), quantitative methylation-specific PCR (QMSP), bisulfite sequencing (BS), microarrays, mass spectrometer, denaturing high-performance liquid chromatography (DHPLC), and pyrosequencing. | 2008-12-18 |
20080311571 | Nucleic Acid Fragments for Detecting Nucleic Acid and Method for Detecting Nucleic Acid - A nucleic acid fragment set according to the present invention comprises a plural number of nucleic acid fragments capable of individually hybridizing with a plural number of target sequences, in which each nucleic acid fragment has a region ligatable with each other and the affinity between such ligatable regions is adjusted to a higher level than the affinity between the target sequence and the nucleic acid fragment. The nucleic acid fragment according to the present invention is for use in a nucleic acid fragment kit. The nucleic acid fragment of the present invention can be used as a probe for detecting nucleic acid or a competitor for detecting nucleic acid. According to the present invention, human leukocyte antigen (HLA) genes, T-cell receptor genes, red blood cell group determining genes, and Rh antigen genes, and the like can be detected at a high accuracy level. | 2008-12-18 |
20080311572 | Methods and Compositions For Correlating Ccl3l1/Ccr5 Genotypes With Disorders - The present invention provides compositions and methods for identifying persons at an increased risk of infection by, transmission of, or accelerated progression of a disease caused by an HIV-1 virus. Diagnostic and therapeutic kits are also provided. | 2008-12-18 |
20080311573 | Compositions, kits, and methods for identification, assessment, prevention, and therapy of breast cancer - The invention relates to newly discovered nucleic acid molecules and proteins associated with breast cancer. Compositions, kits, and methods for detecting, characterizing, preventing, and treating human breast cancers are provided. | 2008-12-18 |
20080311574 | Novel Missense Mutations and Single Nucleotide Polymorphisms in the Rabphillin-3A-Like Gene and Uses Thereof - The invention relates to methods and compositions of matter for determining or predicting aggressiveness of a subject's tumor, for determining a subject's predisposition to cancer, for diagnosing cancer in a subject, and for selecting a therapy for a subject with cancer. Also provided are methods and compositions of matter for determining a Rabphillin-3A-Like gene genotype in a subject and for characterizing a Rabphillin-3A-Like gene in a subject. | 2008-12-18 |
20080311575 | Modulation of Muci-Dependent Anti-Estrogen Resistance - The present invention provides methods for identification and use of compounds that modulate the association of MUC1 with estrogen receptors and thereby antagonize MUC1-related resistance to anti-estrogen treatment. | 2008-12-18 |
20080311576 | Replication competent hepatitis C virus and methods of use - The present invention provides a replication competent hepatitis C virus that includes a heterologous polynucleotide. The invention also includes methods for modifying a hepatitis C virus polynucleotide, selecting a replication competent hepatitis C virus polynucleotide, detecting a replication competent hepatitis C virus polynucleotide, and identifying a compound that inhibits replication of a hepatitis C virus polynucleotide. | 2008-12-18 |
20080311577 | Method for the Identification of Sulfo-Oxidizing Bacteria and for the Monitoring of Elemental Sulfur in the Environment - A method is described for the identification of sulfooxidizing bacteria comprising the extraction of the DNA from environmental samples and the subsequent identification of at least one fragment of the Thio 16S gene or SoxB gene present in these bacteria. The method can be used for determining the level of elemental sulfur in samples of soil. | 2008-12-18 |
20080311578 | System for screening eukaryotic membrane proteins - The present invention provides methods and compositions for expressing eukaryotic membrane proteins. | 2008-12-18 |
20080311579 | Hybridisation beacon and method of rapid sequence detection and discrimination - A method for detecting specific DNA sequences and discriminating single nucleotide polymorphisms (SNPs) using fluorescently labelled oligonucleotide probes is disclosed. Oligonucleotide probes are labelled with reporter molecules preferentially attached to an internal nucleotide residue. The fluorescence emission of oligonucleotide probes varies significantly when in single-stranded and double-stranded states despite the absence of quencher moieties, allowing reliable detection of complementary DNA targets. The melting temperature of probe/target duplexes permits discrimination of targets that differ by as little as a single nucleotide residue, such that polymorphic targets may be discriminated by fluorescence quantitation and Tm. The hybridisation probes of this invention have been demonstrated to accurately identify homozygous and heterozygous samples using a single fluorescent oligonucleotide and direct investigation of saliva with hybridisation probes permits ultra-rapid genotypic analysis within 35-40 minutes. Target detection and SNP discrimination assays have been achieved in homogeneous, heterogeneous, ‘real-time’ and solid-phase formats. | 2008-12-18 |
20080311580 | Novel genes encoding proteins involved in proanthocyanidin synthesis - This inventions provides an isolated protein or polypeptide having activity in the synthesis of proanthocyanidin (PA) polymer from epicatechin or catechin in plants, and which is not naturally regulated by the TT2 or TT8 regulators, or a fragment comprising at least about 10 contiguous amino acids derived from said protein or polypeptide, particularly TDS1, TDS2, TDS3, TDS4, TDS5, or TDS6 protein, or fragment thereof. | 2008-12-18 |
20080311581 | FUNCTIONAL POLYMORPHISMS OF THE INTERLEUKIN-1 LOCUS AFFECTING TRANSCRIPTION AND SUSCEPTIBILITY TO INFLAMMATORY AND INFECTIOUS DISEASES - The invention provides methods and reagents for detecting a polymorphism associated with in an upstream region of the interleukin-1β (IL-B) gene (IL-1B (−3737)) that affects transcription of the gene and susceptibility to inflammatory and infectious diseases such as diffuse coronary artery disease. | 2008-12-18 |
20080311582 | Methods Using Pores - The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto. | 2008-12-18 |
20080311583 | CHROMOSOME MANIPULATION METHOD - A universal method for chromosome modification (deletion of a desired DNA region) which does not require any special enzymes or sequences is provided. Further, a method for determining whether the DNA region to be deleted contains a gene essential for growth of the cell under certain culture conditions is provided by utilizing the chromosome modification method. | 2008-12-18 |
20080311584 | Methods for Detection of Mutations in Myostatin Variants - Methods for detecting allelic variants of the myostatin (growth and differentiation factor-8) gene are provided. Specifically provided are methods of identifying subjects having or having a predisposition for increased muscle mass as compared to subjects having wild-type myostatin. Increased muscle mass is particularly desirable for identification of animals used to produce food products, including bovine, porcine, ovine, avian and piscine species. | 2008-12-18 |
20080311585 | System and method for multiplex liquid handling - The present invention generally relates to microfabricated devices for carrying out and controlling chemical reactions and analysis. In particular, the present invention provides systems, methods, devices and computer software products related to multiplex liquid handling systems utilizing lab cards related to biological assays. | 2008-12-18 |
20080311586 | METHOD FOR MEASURING IN VIVO HEMATOTOXICITY WITH AN EMPHASIS ON RADIATION EXPOSURE ASSESSMENT - The present invention relates a method for assessing in vivo hematotoxicity. The method utilizes differential staining of nucleated and non-nucleated blood cells, and also differential labeling of cells with functional versus dysfunctional mitochondrial membrane potential. Quantitative analyses can be conducted on stained whole blood specimens, and is based on blood cells' fluorescent emission and light scatter properties following exposure to an excitatory light source. The ratio of certain cell populations can be readily measured. Furthermore, it is also possible to express cell population values in terms of number per unit volume. This invention can be used to evaluate the hematotoxicity of drugs, chemicals, radiation, and other exogenous agents, or the effects that a suspected protective agent may have on induced hematotoxicity. Furthermore, the matrix of measurements provided by this invention is useful in estimating radiation dose, i.e., retrospectively. Kits for practicing the invention are also disclosed. | 2008-12-18 |
20080311587 | Use of Genetic Information to Detect a Predisposition for Bone Density Conditions - The invention relates to kits and methods for assessing susceptibility of a human to an undesirable bone density condition, such as osteopenia and osteoporosis, and advising appropriate interventions. The methods involve contemporaneously assessing occurrence in the human's genome of a plurality of polymorphisms (e.g., single nucleotide polymorphisms) that occur in one or more genes associated with bone density regulation and that are associated with a disorder in humans. Preferred assessment and scoring methods are disclosed, as are kits for performing the methods. | 2008-12-18 |
20080311588 | NEUROGENIN - The invention relates to novel neurogenin proteins, nucleic acids and antibodies. | 2008-12-18 |
20080311589 | METHOD OF HIGH-THROUGHPUT SCREENING OF MOLECULES AND COMPOUNDS FOR THEIR EFFECTS ON BIOLOGICAL AND CHEMICAL PROCESSES - The present invention provides a system for high-throughput analysis of chemical compounds. Assays are performed in a high density platform, and compounds having pre-determined desirable effects are identified. Preferably, the compounds have biological effects, more preferably, the assays and detection are performed on whole cells. | 2008-12-18 |
20080311590 | Portable Materials and Methods for Ultrasensitive Detection of Pathogen and Bioparticles - The present invention provides systems for ultrasensitive detection of pathogens and bioparticles. One embodiment of the system comprises an optical detection scheme that allows for the detection of the fluorescence signal of bacteria or other bioparticles in less than about 20 minutes. A microflow channel allows for an assay probing volume of as little as a few picoliters. In one embodiment, the system uses RuBpy dye-doped silica nanoparticles bioconjugated with specific monoclonal antibodies of the target bioparticles. The system allows for the rapid and highly sensitive and specific detection of bacteria or other bioparticles without the need for amplification or enrichment of the sample. | 2008-12-18 |
20080311591 | Differential Immunoassay - The invention provides assay methods and kits that in general measure the level of a first analyte in a sample reduced by the level of a second analyte present in the same sample. In one embodiment, where levels of a first analyte from a first source is desirably determined and first analyte in the sample released from a second source is accompanied by proportional co-release of a second analyte, the assay identifies the level of first analyte released only from the first source. For analytes within bodily fluids, the assay can differentiate between elevated levels of analyte specific to the particular physiological or pathological state and elevated levels not specific to the particular state, providing single tests with diagnostic utility. | 2008-12-18 |
20080311592 | Allosteric Control of Proteins by Manipulating Mechanical Tension - A method of altering the conformation of a polypeptide having a known three-dimensional structure is described. The method comprises attaching a first end of a polymer to a first portion of the polypeptide, attaching a second end of the polymer to a second portion of the polypeptide, and altering the mechanical tension of the polymer, thereby altering the conformation of the polypeptide. The alteration of the conformation of the polypeptide may increase or decrease the binding affinity of the polypeptide for a substrate bound by the polypeptide, or alter the catalytic rate of an enzyme. Typically, the polymer is a polynucleotide or polypeptide. | 2008-12-18 |
20080311593 | METHODS OF DIAGNOSING NON-ALCOHOLIC STEATOHEPATITIS (NASH) - Non-invasive methods for detecting non-alcoholic fatty liver disease (NAFLD) and identifying the presence or absence of non-alcoholic steatohepatitis (NASH) in a subject utilize one or more biomarkers. The methods can differentiate between subjects with NASH and those with simple steatosis. Kits containing one or more agents for measuring the level of the biomarkers can be utilized to perform the described methods. | 2008-12-18 |
20080311594 | USE OF FIBROBLAST GROWTH FACTOR 7 (Fgf7) AND OF THE RECEPTOR Fgfr2b AS BIOMARKERS - The use of fibroblast growth factor Fgf7 and of the corresponding receptor Fgfr2b as biomarker candidates for the progesterone receptor antagonist 11β-(4-acetylphenyl)-17B-hydroxy-17α-(1,1,2,2,2-pentafluoroethyl)estra-4,9-dien-3-one, also known under the designations ZK230211 or ZK-PRA, of the formula | 2008-12-18 |
20080311595 | Rapid fungal detection assay and product - The present invention relates to the use of an assay and product for rapid detection of fungi in a variety of samples suspected of containing fungi, particularly swab samples from the human vaginal area, but also in other environments such as in food and feeds. The product of the invention is suitable for use in a variety of different environments, including in clinical settings, at the point of care, and by patients themselves (bedside kit). | 2008-12-18 |
20080311596 | Genes of Porphyromonas Gingivalis W83 Involved in Invasion of Human Cells - Compositions and methods are provided for detection and treatment of | 2008-12-18 |
20080311597 | Modified Cardiolipin and Uses Therefor - Compositions, methods and devices for the detection of anti-lipoidal antibodies and the diagnosis of disease, for example, syphilis, are described. In particular, oxidized cardiolipins, which may be conjugated with a variety of attachment molecules, such as BSA, KLH, biotin, synthetic protein MAPS, IgY, streptavidin, or avidin, are described. Such oxidized cardiolipin, alone or complexed with one or more attachment molecules, are useful to detect anti-lipoidal antibodies in subjects, for example, when used in lateral flow devices. Lateral flow devices are described that permit the detection of anti-lipoidal antibodies and that permit the co-detection of nontreponemal and treponemal antibodies in biological samples. | 2008-12-18 |
20080311598 | Magnetic Biosensor For Determination of Enzymic Activity - Magnetic sensors are very suitable for use in determination of enzymatic activity. In a preferred embodiment the invention relates to a method for determining activity of an enzyme in modification of substrate ( | 2008-12-18 |
20080311599 | ANTIBODIES AGAINST CASPASE-8, THEIR PREPARATION AND USE - The invention relates to antibodies to a specific region in caspase-8, and to their use. | 2008-12-18 |
20080311600 | ROUNDWORM COPROANTIGEN DETECTION - A composition, device, kit and method for detecting the presence or absence of roundworm in a fecal sample. The composition, device, kit and method of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm. | 2008-12-18 |
20080311601 | Methods for Prediction and Prognosis of Cancer, and Monitoring Cancer Therapy - The present invention relates to biomarkers and the use of biomarkers for the prediction and prognosis of cancer as well as the use of biomarkers to monitor the efficacy of cancer treatment. Specifically, this invention relates to the use of VEGF as a biomarker for multi-kinase inhibitors. | 2008-12-18 |
20080311602 | RAPID ELISA PROCESSES AND RELATED COMPOSITIONS - The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens. | 2008-12-18 |
20080311603 | Diagnostic Test for Inflammatory Endothelial Dysfunctions in Pregnancies - The present invention relates to a diagnostic test for inflammatory endothelial dysfunctions in pregnant women. Particularly, the present invention relates to a method of diagnosing or evaluating the risk of contracting an inflammatory endothelial dysfunction of the maternal compartment comprising the following steps: a) detecting the plasma levels of long pentraxin PTX3 in blood samples taken from a pregnant woman; b) comparing the PTX3 plasma level data, obtained according to step a), with statistically significant PTX3 plasma level data of normal pregnant population. | 2008-12-18 |
20080311604 | Methods for Prediction and Prognosis of Cancer, and Monitoring Cancer Therapy - The present invention relates to biomarkers and the use of biomarkers for the prediction and prognosis of cancer as well as the use of biomarkers to monitor the efficacy of cancer treatment. Specifically, this invention relates to the use of soluble VEGF-R2 as a biomarker for multi-kinase inhibitors. | 2008-12-18 |
20080311605 | KIT AND METHOD TO PERFORM A BIOLOGICAL TEST DESIGNED TO EVALUATE MINERALS POTENTIAL FOR BEING BIOLEACHED - A kit and method to perform a biological assay for evaluating the bioleaching potential of minerals, which allows to know the metallurgic response of minerals for geo-mining-metallurgic short and mid term planning, wherein the kit comprises a propylene tube-type container which has a line that specifies the liquid filling level, where is it possible to place a propylene sphere that floats at the liquid level and it has a plastic twist off cup on the upper extreme, while in the lower extreme or base there is a lyophilized biomass (powder) in charge of the mineral leaching. | 2008-12-18 |
20080311606 | Platelet Activation Markers and Predictors of Desease and of Response to Therapy and for Monitoring Therapeutic Progress - Methods for assessing patient risk for platelet-affected disease states are disclosed. Also disclosed are methods for predicting the appropriateness of platelet antagonist therapy, and for monitoring patients during therapeutic intervention or during a combined regimen of therapy and angioplasty. | 2008-12-18 |
20080311607 | Methods and Compositions for Regulation of Stem Cell Survival, Proliferation, and Differentiation by Protein Ubiquitination - Compositions and methods for regulating in vitro cell growth are disclosed, and for providing undifferentiated stem cells or embryonic cells that are suitable for transplantation into damaged tissues or organs, or for use in tissue repair. A representative method includes causing the overexpression or underexpression of GalT binding protein (GtBP), also referred to as GalT associated protein (GTAP), in a cell such that ubiquitination of at least one cellular protein associated with cell adhesion and/or cell-to-cell interaction is correspondingly increased or decreased, causing inhibition of cell growth when GTAP is overexpressed and causing enhanced cell growth when GTAP is underexpressed by the cell. As a result, growth of the cell is altered or regulated. | 2008-12-18 |
20080311608 | Antagonist of the oncogenic activity of the protein MDM2, and use thereof in the treatment of cancers - The present invention relates to the utilization of a compound capable of antagonizing at least partially the oncogenic activity of the protein Mdm2 for the preparation of a pharmaceutical composition intended more particularly to a treatment of cancers with no p53 context. It further relates to the viral vector comprising a nucleic acid sequence coding for a compound capable of inhibiting at least partially the oncogenic activity of the protein Mdm2, and to a corresponding pharmaceutical composition. | 2008-12-18 |
20080311609 | Novel Molecular Probes - The present invention relates to novel molecular probes having the formula (I) (I) useful for the characterization, detection, localization and isolation of the γ-secretase enzyme. | 2008-12-18 |
20080311610 | Method for the Identification of Macromolecule Targets of Analytes - There is provided a method for the identification of macromolecule targets of analytes such as drugs in biological samples comprising complex mixtures of macromolecules. A biological sample is contacted with one or more analyte and the mixture is resolved such that the analyte and its target are co-eluted and analyzed to identify analyte-target complexes. | 2008-12-18 |
20080311611 | METHODOLOGY FOR VERIFYING CARBON STORAGE IN SEAWATER - A method for verifying carbon storage in seawater to which a growth stimulant has been supplied for stimulating a bloom of nitrogen fixing organisms for enhancing carbon storage therein, comprises selecting a region of seawater including a surface mixed layer, a euphotic zone extending below the surface mixed layer, and a plurality of deeper zones; determining the effect of growth stimulant on the rate of nitrogen fixation and carbon transport in the region; and determining the amount of carbon stored at different depths and projected duration of carbon storage at each of the depths. | 2008-12-18 |
20080311612 | Functional Expression of Higher Plant Nitrate Transporters in Pichia Pastoris - The present invention relates to a system for functional expression of higher plant nitrate transporter (Nrt) genes in | 2008-12-18 |
20080311613 | ARTIFICIAL SKIN SURFACE FILM LIQUIDS - An artificial skin surface film liquid (SSFL) is disclosed that mimics the chemical composition and biological action of both human sweat and sebum. The artificial sweat formulation contains a comprehensive combination of constituents. When combined with the sebum component it is particularly effective at modeling the effect of the skin surface film liquid on agents in contact with the skin. Also provided are methods of making and using the disclosed artificial skin surface compositions. | 2008-12-18 |
20080311614 | METHODS FOR MEASURING PH IN A SMALL-SCALE CELL CULTURE SYSTEM AND PREDICTING PERFORMANCE OF CELLS IN A LARGE-SCALE CULTURE SYSTEM - The present invention is directed to methods/systems for measuring the pH of a cell culture medium in a small-scale system utilizing a pH-sensitive dye. The present invention is also directed to methods for predicting the performance of cells in a large-scale culture system. | 2008-12-18 |
20080311615 | METHOD FOR CHIRAL SEPARATION OF LACTIC ACID ENANTIOMERS - A method for the separation and simultaneous determination of urinary D- and L-lactic acid enantiomers by high performance liquid chromatography tandem mass spectrometry (HPLC/MS/MS). The chiral separation was done using a chirobiotic teicoplanin aglycone column varying mobile phase parameters such as the acetic acid/triethylamine content and the aqueous/organic ratio. | 2008-12-18 |
20080311616 | Hydrophilic IR Transparent Membrane, Spectroscopic Sample Holder Comprising Same and Method of Using Same - The present invention features hydrophilic IR-transparent porous membranes, particularly hydrophilic IR-transparent porous polyethylene membranes and methods of preparing the hydrophilic membranes by treatment of hydrophobic IR-transparent porous membranes with plasma. The present invention further features spectroscopic sample holders which incorporate the hydrophilic IR-transparent porous membranes and methods of identifying bacteria and other microorganisms in samples by infrared spectroscopy. | 2008-12-18 |
20080311617 | Modified Transketolase and Use Thereof - The present invention relates to a improved process for the biotechnological production of compounds for which ribose-5-phosphate, ribulose-5-phosphate or xylulose-5-phosphate is biosynthetic precursor like riboflavin (vitamin B | 2008-12-18 |
20080311618 | Expression System, Components Thereof and Methods of Use - Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from | 2008-12-18 |
20080311619 | Recombinant Carboxypeptidase B - A nucleic acid coding for pro-carboxypeptidase B (Pro-CPB), comprising three segments A, B and C, wherein at least one of the segments has one of the sequences according to SEQ ID No. 1, 2 or 3. | 2008-12-18 |
20080311620 | Novel Genes - The present invention is directed to novel genes mediating the carbon catabolite repression (CCR) of gluconeogenic genes. Furthermore, the polypeptides encoded by said genes as well as the use of said genes in a process for the production of a target fermentation product is provided. Processes for generating such microorganisms are also provided by the present invention. The invention is also related to a genetically engineered microorganism and its use for the production of a target fermentation product, wherein the gluconeogenic genes are relieved from CCR within said microorganism. | 2008-12-18 |
20080311621 | POLYPEPTIDES AND NUCLEIC ACIDS ENCODING SAME - DNAs are provided, whose genes are induced at least by Wnt-1. Also provided are nucleic acid molecules encoding those polypeptides, as well as vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides, and methods for producing the polypeptides. | 2008-12-18 |
20080311622 | ACTIVATABLE RECOMBINANT NEUROTOXINS - Compositions comprising activatable recombinant neurotoxins and polypeptides derived therefrom. The invention also comprises nucleic acids encoding such polypeptides, and methods of making such polypeptides and nucleic acids. | 2008-12-18 |
20080311623 | Plasmid system for multigene expression - The present invention provides a plasmid system which facilitates the construction of a single amplifiable plasmid that, having the potential to accommodate many independent expression cassettes, has the ability to express multi-subunit complex proteins such as antibodies and receptors. | 2008-12-18 |
20080311624 | Chimeric immunogens - Multimeric hybrid genes encoding the corresponding chimeric protein comprise a gene sequence coding for an antigenic region of a protein from a first pathogen linked to a gene sequence coding for an antigenic region of a protein from a second pathogen. The pathogens particularly are parainfluenza virus (PIV) and respiratory syncytial virus (RSV). A single recombinant immunogen is capable of protecting infants and similar susceptible individuals against diseases caused by both PIV and RSV. | 2008-12-18 |
20080311625 | Immortal Pluripotent Stem Cell Line, Cell Lines Derived Therefrom, Methods of Preparing Thereof and Their Uses - The present invention relates to immortal pluripotent stem cells derived from a human leukaemia cell line, preferably a human monocytoid cell line and more preferably the human monocytoid cell line, THP1. The present invention further relates to cell lines derived from the immortal pluripotent stem cell line having the phenotype of cell strains characteristic of human tissues, particularly having a human hepatocyte phenotype, as well as the methods for preparing thereof. The present invention further relates to the use of the derived cell line with a human hepatocytic phenotype for the production of albumin and blood coagulation factors. | 2008-12-18 |
20080311626 | Dna Polymerases Having Strand Displacement Activity - The invention provides novel strand displacement DNA polymerases which can be used in a rapid and efficient strand displacement amplification reactions. The polymerases are significantly more thermostable than prior art polymerase and retain high activity at elevated temperatures. Also disclosed are genes encoding the polymerases and vectors comprising the genes. Representative polymerases of the invention are obtainable from bacterial strains of the species | 2008-12-18 |
20080311627 | Compositions and Methods for Preventing Carry-Over Contamination in Nucleic Acid Amplification Reactions - Particular aspects provide methods for the specific amplification of template DNA in the presence of potentially contaminating PCR products from previous amplification experiments, but wherein the contaminating prior reaction amplificates (carry-over contaminants) are rendered non-amplifiable, based on use of particular contaminant degradation enzymes, and use of at least one primer that is fully complementary to any contaminating prior reaction amplificate nucleic acid, but contains a mismatch with the correspond sequence of the sample template nucleic acid. Additional aspects provide a method for the specific amplification of single-stranded sample template DNA in the presence of potentially double-stranded carry-over products. Further aspects provide methods comprising use of a template-dependent thermostable DNA polymerase enzyme suitable for incorporating ribonucleotides as well as deoxy-nucleotides to provide a chimeric amplificate. After digestion with an RNase, any contaminating prior chimeric amplificate, the RNase is inactivated. These aspects are surprisingly effective alternatives to the carry over protection system known as UNG system, and other art-recognized methods. | 2008-12-18 |
20080311628 | Methods and compositions for rapid amplification and capture of nucleic acid sequences - A method for amplifying a nucleic acid sequence includes the steps of (i) providing a first pair of primers that include one or more uracil nucleotides, the primers being complementary to a portion of a genomic template, (ii) introducing the first pair of primers, the genomic template and a first polymerase into a reaction vessel, (iii) carrying out one or more polymerase chain reaction cycles in the reaction vessel to generate a plurality of first amplicons, and (iv) selectively degrading a portion each first amplicon with a Uracil-DNA Glycosylase to decrease the binding energy of each first amplicon. In one embodiment, the step of selectively degrading includes using a thermostable Uracil-DNA Glycosylase to decrease the binding energy of each first amplicon. In another embodiment, the method also includes the step of adding a second polymerase and a second pair of primers to the reaction vessel to generate a plurality of second amplicons that are different than the first amplicons. Generating the plurality of second amplicons can occur substantially isothermally or non-isothermally. Further, the second pair of primers can be nested primers. | 2008-12-18 |
20080311629 | Development of Pcr Primers and Primer Mixtures For Amplification of Cnp60 Target Sequences - We have developed the primer pair H1511 and H1261 as a replacement for primer pair H279/H280 for specific amplification of cpn60 universal target sequences in genomic DNA or in complex DNA mixtures, including those with high G+C content. | 2008-12-18 |
20080311630 | Dna Constructs for Specific Inhibition of Gene Expression by Rna Interference - The invention relates to expression constructs for targeted inhibition of gene expression and methods for their production and which, after transfection thereof into eukaryotic cells, are suitable for inhibiting in a targeted manner these cells formation of defined proteins by RNA interference, wherein the method is a three step method requiring no PCR steps and is carried out in one reaction vessel in a few hours and are suitable for multiple gene expression inhibition. | 2008-12-18 |
20080311631 | Biosynthetic Production of 4-Amino 4-Deoxychorismate (Adc) and [3R,4R]-4-Amino-3-Hydroxycyclohexa-1,5-Diene-1-Carboxylic Acid (3,4-Cha) - The invention relates to a process for the biosynthetic production of 4-amino-4-deoxychorismate (ADC) performed fermentatively in vivo with a 4-amino-4-deoxychorismate synthase, preferably a PabAB bipartite protein (which may be a fusion protein), at an increased level of activity, thereby obtaining a broth comprising ADC and 4-amino-4-deoxyprephenate (ADP), that are recovered. The invention also relates to a further process of converting the ADP into p-aminophenylalanine. The invention, moreover relates to biosynthetic production of [3R,4R]-4-amino-3-hydroxycyclohexa-1,5-diene-1-carboxylic acid (3,4-CHA), by concerted action of such 4-amino-4-deoxychorismate synthase and of an enzyme capable of converting isochorismate into [5S,6S]-5,6 dihydroxycyclohexa-1,3-diene-1-carboxylic acid (2,3-CHD), preferably a phenazine biosynthesis protein PhzD, including recovery of 3,4-CHA. The invention also relates to expression vectors and host cells for use in any of such processes. The invention further relates to the use of 3,4-CHA as a catalytically active product, in particular as a chiral catalyst. And the invention finally relates to synthesis of oseltamivir phosphate from 3,4-CHA. | 2008-12-18 |
20080311632 | Methods for Producing Methionine by Culturing a Microorganism Modified to Enhance Production of Cysteine - A method of producing methionine, derivatives or precursors thereof comprising culturing a microorganism modified to enhance production of cysteine in a culture medium comprising a source of carbon and a source of sulfur and recovering methionine from the culture medium. A microorganism for fermentative production of methionine or its derivatives in which production of methionine or derivatives thereof, wherein the microorganism enhances production of cysteine. | 2008-12-18 |
20080311633 | Plasmid Vectors For Transformation of Filamentous Fungi - A method for preparing substantially enantiopure 3-hydroxy carboxylic acids or esters of the general formula (III) by reacting racemic oxetan-2-ones of the general formula (I) with compounds R3-OH of the general formula (II) in the presence of a lipase from | 2008-12-18 |
20080311634 | Flavonoids-rich tissue from Belamcanda chinensis and methods for culturing the same - The present invention provides an in vitro flavonoid-rich tissue of | 2008-12-18 |
20080311635 | PROCESS FOR THE PREPARATION OF (S)(+)-3-(AMINOMETHYL)-5-METHYLHEXANOIC ACID - A process for the preparation of (S)(+)-3-(aminomethyl)-5-methylhexanoic acid (pregabalin) of formula (I) or a salt thereof, | 2008-12-18 |
20080311636 | Method for Producing Optically Active Alpha-Hydroxycarboxylic Acid - An efficient method for producing an optically active α-hydroxycarboxylic acid represented by the following general formula (I) [A represents a residue of a 5- or 6-membered cyclic compound, * indicates a carbon atom in the S- or R-configuration, X represents hydrogen atom or an alkyl group having 1 to 4 carbon atoms], which comprises the step of treating a corresponding ester compound (not optically pure) with cell bodies or a culture, or a processed product or an extract thereof of a microorganism of the genus | 2008-12-18 |
20080311637 | Apparatus and methods for ethanol production - Apparatus and methods for ethanol production use shock waves and pulsed electric fields to increase the conversion of starch and/or cellulosic material into sugar. The shock waves or the pulsed electric fields may also control bacteria levels in the ethanol production facility. The shock waves and the pulsed electric fields may be generated, at least in part, by a pulsed electric field generator such as a Marx generator, which may have one or more semiconductor switches. | 2008-12-18 |
20080311638 | Shock wave apparatus and methods for ethanol production - Apparatus and methods for ethanol production use shock waves to increase the conversion of starch and/or cellulosic material into sugar. The shock waves may also control bacteria levels in the ethanol production facility. The shock waves may be generated by a shock wave generator that includes a pulsed electric field generator such as a Marx generator, which may have one or more semiconductor switches. | 2008-12-18 |
20080311639 | Pulsed electric field apparatus and methods for ethanol production - Apparatus and methods for ethanol production use pulsed electric fields to increase the conversion of starch and/or cellulosic material into sugar. The pulsed electric fields may also control bacteria levels in the ethanol production facility. The pulsed electric fields may be generated by a pulsed electric field generator such as a Marx generator, which may have one or more semiconductor switches. | 2008-12-18 |
20080311640 | Anaerobic Production of Hydrogen and Other Chemical Products - Described herein are methods for producing chemical products by anaerobically fermenting a particular biomass using anaerobic bacteria. Such chemical products include hydrogen and other gases, acetic acid and other volatile organic acids, solvents, solids, and salts of volatile organic acids. | 2008-12-18 |
20080311641 | Crystallization of enzyme and method for producing same - The present invention provides a method of crystallizing of enzymes. The method is for rapidly crystallizing enzymes from impure mixtures. The method is simple and cheap, and it is compatible to industrial requirements. T1 lipase was able to form crystals at low protein concentration (2.5 mg/ml) in a day. High temperature crystallization was obtained from the method. The present invention also relates to a composition of a crystallized lipase produced from the said method. | 2008-12-18 |
20080311642 | Methods of Purifying Chondroitinase and Stable Formulations Thereof - An aspect of the present invention relates to stable formulations of chondroitinase and to methods of purifying chondroitinase. The methods of purifying chondroitinase includes the steps of extracting the enzyme from a cell, separating the chondroitinase from the crude cell extract using cation-exchange chromatography, removing impurities through gel filtration chromatography, and removing endotoxin through an anion-exchange membrane to produce a purified chondroitinase. | 2008-12-18 |
20080311643 | Novel Pseudomonas aeruginosa: Bacteriophage and Uses Thereof - The present invention is directed to isolated bacteriophage having strong lytic activity against strains of | 2008-12-18 |
20080311644 | Amino Methylated 2-Pyridinones - Novel amino methylated 2-pyridinones, precursors, intermediates, and derivatives; the methods for the preparation of the same; uses of the same for inhibiting pili formation in bacteria; and pharmaceutical compositions comprising these compounds are described in this application. The present compounds may be employed to inhibit biofilm formation and thereby inhibit adherence of bacteria to a host cell. | 2008-12-18 |
20080311645 | Method of Culturing a Microorganism - A method of culturing a microorganism capable of producing at least any one of a highly unsaturated fatty acid or a compound containing a highly unsaturated fatty acid as a constituent fatty acid in a culture medium containing at least a carbon source and a nitrogen source, using an aeration-agitation culture device capable of adjusting and controlling an agitation power and an aeration amount. The method comprises the steps of performing mechanical agitation for a predetermined time after start of culture, where the agitation power per unit liquid amount is 269 (W/m | 2008-12-18 |
20080311646 | Carbon Supply Device for Cultivating Miro Algae in Large and Its Application Method and Use - Disclosed herein is a carbon supply device for supplying carbon dioxide during large scale cultivation of micro-algae in open pond, and its application method and use. The device comprises a trap container in which a partition plate is installed vertically and has a 10-50 cm gap from the container bottom, and the partition plate is higher than the wall of the trap container; and a gas distributor is positioned at the bottom of the container. In use, the trap carbon supply device is embedded in bottom of an open pond, wherein the partition plate is above the liquid level of the culture solution in the open pond such that the culture solution driven by a stirrer flows into the trap carbon supply device from one side thereof, and flows out of the device from the other side thereof, wherein the culture solution is mixed with carbon dioxide at the bottom of the container. | 2008-12-18 |
20080311647 | Method of Biotreating a Solid Material Comprising an Organic Compound - A method of biotreating a solid material including an organic compound is provided. According to the method, a nonstirred bioreactor having a void volume of greater than or equal to 25% with a mixture including a solid material to be biotreated and a plurality of coarse substrates having a particle size greater than or equal to about 0.6 cm is formed. The solid material to be biotreated includes an organic material. The mixture includes sufficient coarse substrates to provide the reactor with at least 100 square meters of surface area per cubic meter of reactor space. The solid material in the bioreactor is biotreated until the organic material within the bioreactor is reduced to a desired concentration. | 2008-12-18 |
20080311648 | Biosensor for analyzing quantitatively analyte with a predetermined size and larger than, and manufacturing method thereof - The present invention discloses a biosensor for quantitatively analyzing a bio-material and a manufacturing method thereof. The biosensor has an exposed conductive region of a few-nanometer scale distributed on an insulated metallic substrate in a desired pattern or randomly. The quantitative analysis of protein can be carried out by means of simplified procedures, without the necessity of rinsing out a signal-producing material, which is non-specifically bonded to the materials to be analyzed. The biosensor utilizes only the size of the molecules, and thus can be universally used for the analysis of bio-materials. A selective and separate analysis can be realized in which interference caused by other materials is significantly reduced. | 2008-12-18 |
20080311649 | Pressurized flexible tubing system for producing Algae - An apparatus for producing algae circulates algae fluid through flexible reactor tubing that is at least partially translucent to sunlight. The reactor tubing lies flat when not pressurized. Preferably, the reactor tubing is made of clear polyethylene with UV inhibitors, the polyethylene being between 6 and 15 mil thick. The reactor tubing preferably has a substantially circular cross-section with a 6 inch diameter and is preferably 1250 feet long. Gas relief valves allow gases generated during algae production to escape from the reactor tubing. CO | 2008-12-18 |
20080311650 | Bioreactor for Tissue Engineering - The present invention relates to a method and apparatus for growing cells in a three-dimensional scaffold. Relative movement of the scaffold and an end cap of the culture chamber results in circulation of the growth medium through the scaffold. The invention is also suited for introduction of cells into a scaffold. The scaffold may be any sort of natural or synthetic material that will support cellular life, including a tissue. | 2008-12-18 |
20080311651 | Instrumentality for Sequestering Liquids Based on Density: Method and Apparatus - An apparatus and method for collecting whole blood and then separating it into components for subsequent use or storage. A self-contained bag set is used to collect the sample, which may then be placed into a device adapted to fit into a centrifuge for separation of components. Each component is then sequentially extracted according to density, with a sensor present in the device to control the operation of valves directing the collection of each component. The sensor may be reading one or more of the following characteristics: infrared, optics, density, weight, radioactive, fluorescence, color, magnetism, ultrasonic, capacitance wherein the characteristic is inherent in the blood and blood component or is an additive. Each component may then be separated into its own storage container. The preferred sensors include optics and weight. Besides blood density separation, the device may contain a solution including cells, proteins, subcellular particles or viruses which may be mixed with affinity media or antibodies prior to separation. | 2008-12-18 |
20080311652 | MAGE-C2 antigenic peptides and uses thereof - This invention relates to isolated peptides derived from MAGE-C2, nucleic acid molecules that encode MAGE-C2 and the isolated peptides derived from MAGE-C2, expression vectors comprising the nucleic acid molecules, host cells transformed or transfected with the nucleic acid molecules or the expression vectors, and to tetramers comprising the peptides, HLA molecules, β | 2008-12-18 |
20080311654 | Chicken leukemia inhibitory factor (LIF) and gnne thereof - The present invention is to provide a chicken LIF gene. Based on this genetic information, LIF protein derived from the chicken can be stably supplied and it solves the problems of the creation of transgenic chickens in the past. In addition, the present invention provides not only transgenic chickens for testing purposes but also supplies the first practical transgenic stock animals. The present invention relates to leukemia inhibitory factor (LIF) shown in sequence No. 2, and the gene that encodes thereof shown in sequence No. 1, and a manufacturing method of chicken LIF. In addition, the present invention pertains to a differential preventer of the chicken differentiable cell, and a method of chicken differential prevention and a culturing method for the chicken differentiable cell using thereof, and a medium comprising thereof. | 2008-12-18 |
20080311655 | Heterodimeric fusion proteins useful for targeted immune therapy and general immune stimulation - Disclosed are methods for producing fusion proteins with the heterodimeric cytokine, interleukin-12. In order to insure that the proper ratio of fused and non-fused subunits are obtained in the fusion protein, a specific stepwise approach to genetic engineering is used. This consists of first expressing the non-fused p40 IL-12 subunit in a production cell line, followed by or simultaneously expressing in the same cell, a second recombinant fusion protein consisting of the fused polypeptide linked by a peptide bond to the p35 subunit of IL-12. Molecules containing the p35 fusion protein cannot be secreted from the transfected mammalian cell without first complexing in a one to one ratio with the p40 subunit, thus ensuring the production of active heterodimeric fusion proteins. | 2008-12-18 |
20080311656 | Meiosis arrest in oocytes in vitro - The method for in vitro synchronisation of nuclear and cytoplasmatic maturation of GV oocytes from domestic animals or from primates can be improved if a phosphodiesterase type 3 inhibitor is added to the medium after collection of the oocytes and, thereafter, said phosphodiesterase type 3 inhibitor is removed to allow the nuclear maturation to proceed. | 2008-12-18 |
20080311657 | Killing Human Lymphoma and Leukemia Cancer Cells and Tcr-Activated Normal Human Cells By Dopamine D1r Agonists - The dopamine D1/D5 receptor is highly over-expressed in various types of human and animal leukemia, lymphoma and activated T-cells. The dopamine D1 receptor is also expressed in dramatically elevated or even moderate levels in other types of cancer cells. Selective dopamine D1 receptor agonists, such as fenoldopam mesylate, rapidly, potently and selectively kill such human and animal T-cells expressing the dopamine D1 receptor. Thus, selective dopamine D1/5 receptor agonists may be used to treat lymphoma, leukemia and other cancers of the immune system, and T-cell mediated autoimmune diseases and other diseases caused by over-activated inflammatory T-cells (such as chronic inflammation), or graft versus host diseases (GVHD) or graft rejection, or by any other cell types expressing the dopamine D1 receptor, by killing the disease-causing cells. The selective dopamine D1/5 receptor agonists can be used for these purposes either in vivo or in vitro, such as to purge a given cell population from undesired leukemia, lymphoma or activated T-cells prior to further use. | 2008-12-18 |
20080311658 | COMBINED HAIRPIN-ANTISENSE COMPOSITIONS AND METHODS FOR MODULATING EXPRESSION - A nucleotide construct comprising a nucleotide sequence that forms a stem and a loop, wherein the loop comprises a nucleotide sequence that modulates expression of a target, wherein the stem comprises a nucleotide sequence that modulates expression of a target, and wherein the target modulated by the nucleotide sequence in the loop and the target modulated by the nucleotide sequence in the stem may be the same or different. Vectors, methods of regulating target expression, methods of providing a cell, and methods of treating conditions comprising the nucleotide sequence are also disclosed. | 2008-12-18 |