50th week of 2009 patent applcation highlights part 46 |
Patent application number | Title | Published |
20090305318 | Diagnostic Tests of Substance Use Disorders - The present invention relates to compositions and methods for identifying and quantifying platelet proteins. As certain behaviors and medical conditions alter the quantity of various platelet membrane proteins, the tools of the present invention are suitable for identification of biomarkers of these bodily states. For instance, the present invention provides methods and compositions for determining whether an individual is using alcohol or other licit or illicit drugs at levels hazardous or harmful to their health. The invention also provides methods for identifying individuals who would benefit from or who may be harmed by specific medications or therapies. | 2009-12-10 |
20090305319 | Apparatus and methods for monitoring the status of a metabolically active cell - An apparatus and methods for monitoring the status of a cell that consumes oxygen. In one embodiment of the present invention, the method includes the steps of confining the cell in a sensing volume, measuring dynamically intracellular or extracellular signaling of the cell, and determining the status of the cell from the measured intracellular or extracellular signaling of the cell. | 2009-12-10 |
20090305320 | Method of detecting activation of notch signal transmission system - The purpose of the present invention is to provide detection methods of Notch signaling activation for detecting the activation of the Notch signaling in living cells simply and conveniently. The expression of a fluorescent protein Venus in a transgenic cell into which a vector having the fluorescent protein Venus gene which is controlled by the wild-type Hes-1 gene promoter has been introduced is compared with the expression of a fluorescent protein Venus in a transgenic cell into which a vector having the fluorescent protein Venus gene controlled by a mutated Hes-1 gene promoter which is not controlled by an activated Notch protein has been introduced, and a transgenic cell in which a signal by the expression of Venus introduced by the vector having the wild-type Hes-1 promoter is observed and in which a signal by the expression of Venus introduced by the vector having the mutated Hes-1 gene promoter which is not controlled by the activated Notch protein is not observed is identified. | 2009-12-10 |
20090305321 | Assays for Allosteric Modulators of G-Protein Coupled Receptors (GPCRs) - The present invention relates to G protein-coupled receptors (GPCRs) and allosteric modulators thereof. More specifically, the invention relates to allosteric modulators of GPCRs that interact at an intracellular binding site. It also relates to methods for designing or identifying small molecule allosteric modulators, including assays (such as competitive binding assays) and methods employing a homology model for the GPCR intracellular site. | 2009-12-10 |
20090305322 | Isotope Detection and Uses Thereof - A method is disclosed for measuring the hydrogen and/or oxygen isotope ration of intracellular water in | 2009-12-10 |
20090305323 | LIPIDOMICS APPROACHES FOR CENTRAL NERVOUS SYSTEM DISORDERS - The present invention has utilized the power of lipidomics to profile lipid metabolites and to characterize changes in lipid metabolism as they relate to CNS disorders. Lipidomic signatures can guide the development of diagnostic, prognostic and surrogate markers for CNS disorders; identification of new targets for drug design based on highlighted perturbed pathways; stratify patients with CNS disorders as to which pathways are impaired, and facilitate the determination of which patients with CNS disorders are candidates for a particular therapy, i.e. provide the tools for a personalized approach to therapy; identify which patients are responding or are developing side effects to a treatment; design of modified antipsychotics that have less metabolic side effects and enhanced activity; overcome the lag phase in response to some treatments; and find better combination therapies for CNS disorders that target the pathways that are impaired (e.g., impairments in lipid and/or carbohydrate metabolism). | 2009-12-10 |
20090305324 | CYTOTOXIC T-CELL EPITOPE PEPTIDES THAT SPECIFICALLY ATTACK EPSTEIN-BARR VIRUS-INFECTED CELLS AND USES THEREOF - The present inventors introduced mRNAs for the Epstein-Barr virus proteins LMP1 and EBNA1 into antigen-presenting cells, and as a result, demonstrated that the cells induced Epstein-Barr virus-specific cytotoxic T cells. The present inventors also demonstrated that the cytotoxic T cells recognized epitope peptides presented via HLA-A*0206, HLA-Cw*0303, or HLA-Cw*0304, inhibited the outgrowth of Epstein-Barr virus-infected B cells, and lysed Epstein-Barr virus-infected NK lymphomas and NK cells. | 2009-12-10 |
20090305325 | Method for Preservation of Human Hematopoietic Stem or Progenitor Cells - Maintenance of quiescent hematopoietic stem and progenitor cells (HSPC) in culture without the addition of exogenous growth factors is an important aspect in clinical hematology. A recent report described the ability of Flt3 receptor-interacting lectin (FRIL) in the maintenance of cord blood (CB) derived progenitors in vitro. Since FRIL is a mannose binding lectin, the effectiveness of four such lectins of well-characterized specificities on the preservation of HSPC of CB origin have been examined. The HSPC preservation activity of lectins was assessed by in vitro colony forming unit (CFU) and long-term culture initiating cell (LTC-IC) assays. It was found that all four lectins had a HSPC preservation activity at least up to 30 days in culture without addition of exogenous growth factors. The results indicate that use of such lectins may provide a cost effective method of HSPC maintenance for clinical use. | 2009-12-10 |
20090305326 | MICROFLUIDIC DEVICE AND METHOD FOR COUPLING DISCRETE MICROCHANNELS AND FOR CO-CULTURE - A microfluidic device and method is provided for coupling discrete channels and for co-culture. The microfluidic device includes first and second bodies. Each body has a bottom surface and defines a channel. The channel in each body includes an inlet and an outlet communicating with the bottom surface. A first fluid, such as a first cell suspension, is provided within the channel of the first body and a second fluid, such a second cell suspension, is provided within the channel of the second body. The first and second bodies are movable between a first position wherein the outlet of the channel of the first body is spaced from the inlet of the channel of the second body and a second position wherein the fluid at the outlet of the channel of the first body communicates with the fluid at the inlet of the channel of the second body. | 2009-12-10 |
20090305327 | Mass Spectrometric Determination Of Blood Enzyme Activity - The invention relates to the determination of the nature and strength of enzymatic activity in blood using mass spectrometric measurement of a profile of the reaction products. The determination of the enzymatic activity can be used for medical diagnostics, for example, and also to check the effectiveness of medication. The invention provides a method whereby adding probe substances usually not present in blood offers standardized substrates for measuring the enzymatic activity. The probe substances may be added to whole blood, plasma, or serum. The mass spectrometric measurement of the reaction products, after their reversible immobilization on actively binding surfaces of solids, for example, can deliver biomarker patterns of the reaction products which may be indicators for metabolic anomalies or diseases, since these are often accompanied by the formation or activation of characteristic enzymes. | 2009-12-10 |
20090305328 | METHOD OF IMPROVEMENT OF ORGANISMS USING PROFILING THE FLUX SUM OF METABOLITES - The present invention relates to a method for improving an organism through the profiling of flux sum of metabolites, and more particularly to a method for screening key metabolites, the method comprises: plotting a profile between objective functions based on useful substance formation rate as a main function through an algorithm perturbing other functions influencing the production of useful substance; determining the utilization (flux sum (Φ)) of all metabolites from the profile; and screening key metabolites, which show an increase in flux sum (Φ) with an increase in useful substance formation rate. The present invention also relates to a method for improving an organism producing a useful substance, the method comprises introducing and/or amplifying genes associated with the screened key metabolites or introducing the genes from the outside into the organism. According to the disclosed invention, the metabolic utilization (flux sum; Φ) of specific metabolites according to an increase in useful substance formation rate can be predicted, so that key metabolites in increasing the production of a useful substance can be screened. Also, it is possible to increase the production of a useful substance through the method of improving a target organism by introducing and/or amplifying genes associated with the screened metabolites or through the method of supplying the metabolites during the culture of the organism. | 2009-12-10 |
20090305329 | INTRACELLULAR TARGETING OF MOLECULES - The present invention provides methods for intracellular and/or nuclear targeting of an agent capable of specifically binding to syndecan-4. The present invention further provides methods for the modification of the intracellular and/or nuclear targeting of said agent, as well methods for identifying compounds capable of modifying the syndecan-4 delivery pathway. The present invention further provides experimental kits to perform the methods according to the invention. | 2009-12-10 |
20090305330 | METHOD AND APPARATUS FOR THE DETECTION OF LIVING PHYTOPLANKTON CELLS IN WATER - The invention related to a method and an apparatus for detecting living phytoplankton cells and/or microorganisms in or out of water, particularly ballast water, bodies of water, sewage, or water in swimming and bathing devices. Said method is characterized by the following steps:—the variable fluorescence (Fv) is calculated by forming the difference between the maximum fluorescence (Fm) and the minimum fluorescence (Fo) in a measuring space or detecting part or all of the dynamic shape of a fluorescence induction curve in a measuring space, particularly measuring; and—calculating the number of living phytoplankton cells and/or microorganisms of a reference species in the measuring space in accordance with the variable fluorescence (Fv). | 2009-12-10 |
20090305331 | Kits and methods for determining colon cleanliness - Method and kits for determining a cleanliness level of a colon of a subject are provided. The methods and kits of the present invention determine a parameter associated with colon cleanliness in a sample obtained from the subject and associate the parameter with the cleanliness level of the colon of the subject. | 2009-12-10 |
20090305332 | Method and Apparatus for Determining an Analyte in a Liquid Sample - A method for the determination of an analyte in a liquid sample, especially a body liquid sample, with the aid of an analytical apparatus. The apparatus receives a first measuring signal s | 2009-12-10 |
20090305333 | Promoting Axon Regeneration in the Adult CNS through Control of Protein Translation - Survival of, or axon regeneration in a lesioned mature central nervous system (CNS) neuron is promoted by (a) contacting the neuron with a therapeutically effective amount of an exogenous activator of protein translation; and (b) detecting the resultant promotion of the survival of, or axon regeneration in the neuron. | 2009-12-10 |
20090305334 | METHOD FOR MAKING A BIOLOGICAL INDICATOR FOR USE WITH VAPOROUS MICROBIAL DEACTIVATING AGENTS - A biological indicator and method of making same. The biological indicator includes a carrier having a recess formed therein in order to restrict movement of an inoculum deposited onto the carrier. The inoculum includes microorganisms (e.g., bacterial spores) suspended in a suspension medium. The microorganisms are prepared by removing extraneous material and subjecting the microorganisms to sonication to break up agglomerations. The suspension medium includes a wetting agent to reduce surface tension, thereby facilitating flow of the suspension medium to prevent stacking of microorganisms on the surface of the carrier, and to allow the inoculum to more evenly “plate out” on carrier surfaces. The carrier, with inoculum deposited thereon, is enclosed in an envelope made of a material permeable to a vaporous deactivating agent (e.g., vaporized hydrogen peroxide, ozone, chlorine dioxide, ethylene oxide, etc.), thereby facilitating exposure to the vaporous deactivating agent. | 2009-12-10 |
20090305335 | Method for analysing a sample - This invention relates a method for analysing a biological sample by imaging, wherein sample processing and detection and optionally pre-treatment is performed in a single container. The invention further relates to a kit for use in said method. | 2009-12-10 |
20090305336 | DEVICES AND METHODS FOR SAMPLING MICROBIAL FLORA - Methods, devices and kits for sampling for a microorganism or microbial population on the digits of the extremities of an individual are described. In embodiments of the invention, the device comprises a container, such as a cell culture plate, with a growth medium that is delineated into multiple quadrants, which can be visualized through the growth medium, to aid in the placement of a digit within a designated location on the growth medium. | 2009-12-10 |
20090305337 | SAMPLE PROCESSING SYSTEM AND METHOD - Provided is a system and method for staining of one or more samples, including providing one or more self-contained sample processing receptacles, each of the one or more self-contained sample processing receptacles configured to be inserted into an auto-staining instrument; and enabling one of one or more staining procedures appropriate for the one or more samples as a function of a choice of self-contained sample processing receptacle, each of the one or more self-contained sample processing receptacles configured to process each inserted sample of the one or more samples within the self-contained sample processing receptacle. | 2009-12-10 |
20090305338 | Plant cell lines established from the medicinal plant veratrum californicum - The present invention relates to the field of plant secondary metabolites. More particularly plant cell lines are established from the medicinal plant | 2009-12-10 |
20090305339 | Polypeptides With Laccase Activity - The invention relates to a new laccase from rumen, namely the RL5 laccase. In particular, the invention relates to a polypeptide comprising the amino acid sequence shown in | 2009-12-10 |
20090305340 | ALTERED PEPTIDE LIGANDS OF GAD65 - Modified GAD65 compositions antagonize the activities of islet-specific T cells that contribute to the progression of one or more autoimmune disorders. The compositions are also antagonistic in humanized mice that express human HLA alleles associated with increased-risk of Type 1 diabetes. | 2009-12-10 |
20090305341 | HCL-K1 Polypeptide Which Offers Collectin Activity - Disclosed is the novel hCL-K1 polypeptide which offer collectin activity. This polypeptide consists of consecutive 271 amino acids set out in SEQ ID NO: 2 and does not bind to both maltose and N-acetylgalactosamine. | 2009-12-10 |
20090305342 | Solubilization and Purification of a Target Protein fused to a Mutant Maltose-Binding Protein - Methods and compositions are provided for increasing at least one of: (i) binding affinity of a target protein for a maltodextrin substrate and/or (ii) solubility of a target protein. The methods and compositions relate to a modified maltose-binding protein. | 2009-12-10 |
20090305343 | Method For Expressing Polypeptides In Eukaryotic Cells Using Alternative Splicing - This invention relates to an expression cassette for expressing polypeptides in eukaryotic cells using alternative splicing. The expression cassette comprises in 5′ to 3′ downstream direction: a promoter; a sequence transcribed in a 5′ untranslated region (5′UTR); a donor splice site; an intron; a first acceptor splice site; a first cistron encoding a first polypeptide; a second acceptor splice site; a second cistron encoding a second polypeptide; an internal ribosome entry site (IRES) operably linked to a selection marker; and a sequence transcribed in a 3″ untranslated region (3′UTR) including a polyadenylation signal, wherein the polyadenylation signal is unique. | 2009-12-10 |
20090305344 | CHIMERIC ALPHAVIRUS REPLICON PARTICLES - Chimeric alphaviruses and alphavirus replicon particles are provided, including methods of making and using same. Specifically, alphavirus particles are provided having nucleic acid molecules derived from one or more alphaviruses and structural proteins (capsid and/or envelope) from at least two or more alphaviruses. Methods of making, using, and therapeutic preparations containing the chimeric alphavirus particle, are disclosed. | 2009-12-10 |
20090305345 | POLYMERASE - The present invention relates to an engineered polymerase characterized in that the polymerase exhibits an enhanced ability to process nucleic acid in the presence of environmental and biological inhibitors compared to wild type DNA polymerase. | 2009-12-10 |
20090305346 | Engineered cleavage half-domains - Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence. | 2009-12-10 |
20090305347 | Pichia Pastoris P1R1 Secretion Signal Peptide for Recombinant Protein Expression and Pichia Pastoris P1R1 and P1R2 Anchor Domain Peptides for Recombinant Surface Display - The present invention is directed to recombinant nucleic acid constructs comprising nucleic acid sequences encoding a | 2009-12-10 |
20090305348 | DIAGNOSTIC TEST FOR VITAMIN B12 - An isolated nucleotide sequence or fragment thereof encoding the porcine intrinsic factor, wherein the porcine intrinsic factor comprises an amino acid sequence having at least 85% amino acid sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 6, and SEQ ID NO: 9. The invention also encompasses an isolated nucleic acid sequence or fragment thereof comprising, or complementary to, a nucleotide sequence having at least 85% nucleotide sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 4, and SEQ ID NO: 7. The porcine intrinsic factor can be use is an assay to determine the quantity of vitamin B | 2009-12-10 |
20090305349 | EXPRESSION OF SOLUBLE FACTOR VIII PROTEINS IN BACTERIA - The present invention provides enhanced methods of producing soluble Factor VIII proteins in microorganisms that have an oxidizing environment. | 2009-12-10 |
20090305350 | Gene Differentially Expressed in Breast and Bladder Cancer and Encoded Polypeptides - The present invention relates to a novel human gene that is differentially expressed in human carcinoma. More specifically, the present invention relates to a polynucleotide encoding a novel human polypeptide named C35 that is overexpressed in human breast and bladder carcinoma. This invention also relates to C35 polypeptide, in particular C35 peptide epitopes and C35 peptide epitope analogs, as well as vectors, host cells, antibodies directed to C35 polypeptides, and the recombinant methods for producing the same. The present invention further relates to diagnostic methods for detecting carcinomas, including human breast carcinomas. The present invention further relates to the formulation and use of the C35 gene and polypeptides, in particular C35 peptide epitopes and C35 peptide epitope analogs, in immunogenic compositions or vaccines, to induce antibody or cell-mediated immunity against target cells, such as tumor cells, that express the C35 gene. The invention further relates to screening methods for identifying agonists and antagonists of C35 activity. | 2009-12-10 |
20090305351 | METHOD FOR PREPARING SOLUBLE AND ACTIVE RECOMBINANT PROTEINS USING PDI AS A FUSION PARTNER - The present invention relates to a method for producing a recombinant protein capable of increasing expression rate of a target protein and also improving solubility and folding of the expressed target protein using a modified protein disulfide isomerase (PDI) as a fusion partner, and an expression vector containing the modified PDI gene as a fusion partner. The method for preparing a recombinant protein using a modified PDI as a fusion partner according to the present invention may solve the problems concerning a low yield and solubility and folding that conventional fusion partners have, and be widely used for protein drug and industrial protein production. | 2009-12-10 |
20090305352 | ACTIVATED COLLAGEN SCAFFOLD MATERIALS AND THEIR SPECIAL FUSED ACTIVE RESTORATION FACTORS - Provided are activated collagen scaffold materials as well as their special fused active restoration factors useful for promoting tissue repair, such as bone damage repair or nerve injury repair. The special fused active restoration factors are fusion proteins comprising a collagen-binding domain (CBD) at N-/C-terminus of cytokines, wherein the collagen-binding domain is a polypeptide consisting of 7-27 amino acid residues with a conservative sequence shown in SEQ IN NO:1 at N-terminus. | 2009-12-10 |
20090305353 | Mutant firefly luciferase, gene, recombinant vector, transformant, and method for production of mutant firefly luciferase - The present invention provides a mutant firefly luciferase consisting of a mutant amino acid sequence derived from the amino acid sequence of a wild-type firefly luciferase by at least substitution (a), (b), or (c) below and having luminescence intensity higher than that of the wild-type firefly luciferase. According to the present invention, a mutant firefly luciferase with increased luminescence intensity compared with that of wild-type firefly luciferases is provided, regarding which (a) through (c) are as follows:
| 2009-12-10 |
20090305354 | PROCESS FOR PREPARING HUMAN G-CSF - The present invention discloses an improved process for the production of G-CSF in high yield via a high salt-induced increase in plasmid stability during the production phase. | 2009-12-10 |
20090305355 | Method for Syngas-Production from Liquefied Biomass - The present invention relates to methods for syngas-production from biomass enabling the conversion of pre-treated biomasses having a high dry-matter content into electricity or oil-based products such as petrol, diesel, chemicals and plastics through the formation of syngas. The biomasses are converted into a biomass slurry having a suitable particle size and dry-matter content for optimal feeding and gasification in a pressurised gasifier. | 2009-12-10 |
20090305356 | METHODS AND APPARATUS FOR THE USE OF ULTRASONIC ENERGY TO IMPROVE ENZYMATIC ACTIVITY DURING CONTINUOUS PROCESSING - Described herein are methods and devices for increasing enzymatic activity during continuous processing by applying ultrasonic energy. | 2009-12-10 |
20090305357 | CHONDROITIN POLYMERASE AND DNA ENCODING THE SAME - A chondroitin polymerase having such properties that it transfers GlcUA and GalNAc alternately to a non-reduced terminal of a sugar chain from a GlcUA donor and a GalNAc donor, respectively, and the like; and a process for producing the chondroitin polymerase. | 2009-12-10 |
20090305358 | DNA Vector Production System - The invention discloses the production of double stranded DNA (dsDNA) vectors capable of delivering nucleic acids, including cDNA, antisense, ribozyme, and small interference RNA into cells. The invention also describes nucleic acid constructs as well as methods for the production of the dsDNA vectors. | 2009-12-10 |
20090305359 | METHOD FOR PRODUCING CIRCULAR DUPLEX POLYNUCLEOTIDES FROM LINEAR DUPLEX POLYNUCLEOTIDES AND APPLICATIONS THEREOF - Methods are provided for making a circular duplex polynucleotide. Such methods can include providing a mixture of sequence specific linear duplex polynucleotides and denaturing and reannealing the polynucleotide mixture under conditions such that some of the polynucleotides form circular duplexes that comprise the desired polynucleotide. | 2009-12-10 |
20090305360 | SACCHARIFICATION ENZYME COMPOSITION AND METHOD OF SACCHARIFICATION THEREOF - The present disclosure relates to a | 2009-12-10 |
20090305361 | Starch Process - The present invention relates to a process for enzymatic hydrolysis of granular starch into a soluble starch hydrolysate at a temperature below the initial gelatinization temperature of said granular starch. | 2009-12-10 |
20090305362 | Process for preparing uronic acid oligosaccharides by extrusion - The present invention provides processes for the manufacture of uronic acid oligosaccharides using extrusion. The uronic acid polymers are extruded with an enzyme, under basic conditions (pH>9) and/or under high shear. | 2009-12-10 |
20090305363 | ENHANCED PYRUVATE TO ACETOLACTATE CONVERSION IN YEAST - A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activity and reduced glycerol-3-phosphate dehydrogenase activity. Production of compounds having acetolactate as an upstream intermediate benefit from the increased conversion of pruvate to acetolactate in the described strains. | 2009-12-10 |
20090305364 | MICROORGANISMS FOR THE PRODUCTION OF ADIPIC ACID AND OTHER COMPOUNDS - The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam. | 2009-12-10 |
20090305365 | FAD4, FAD5, FAD5-2, AND FAD6, NOVEL FATTY ACID DESATURASE FAMILY MEMBERS AND USES THEREOF - The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturase family members. The invention also provides recombinant expression vectors containing desaturase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., DHA. | 2009-12-10 |
20090305366 | Production Of Peracids Using An Enzyme Having Perhydrolysis Activity - A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided. | 2009-12-10 |
20090305367 | PROCESS FOR PREPARING LONG-CHAIN DICARBOXYLIC ACIDS - The present invention particularly discovered strains that are capable of producing a long-chain dicarboxylic acid by culturing microorganisms belonging to | 2009-12-10 |
20090305368 | METHOD FOR PRODUCING HYDROXYCARBOXYLIC ACID BY REGENERATING COENZYME - Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to regenerate oxidized-type nicotinamide adenine dinucleotide by being provided with an enhanced NADH dehydrogenase function by introducing a gene encoding NADH dehydrogenase into a microorganism. | 2009-12-10 |
20090305369 | DELETION MUTANTS FOR THE PRODUCTION OF ISOBUTANOL | 2009-12-10 |
20090305370 | METHOD FOR PRODUCING BUTANOL USING TWO-PHASE EXTRACTIVE FERMENTATION - A method of making butanol from at least one fermentable carbon source that overcomes the issues of toxicity resulting in an increase in the effective titer, the effective rate, and the effective yield of butanol production by fermentation utilizing a recombinant microbial host wherein the butanol is extracted into specific organic extractants during fermentation | 2009-12-10 |
20090305371 | METHOD USING INFRARED LIGHT FOR ETHANOL PRODUCTION - The present invention provides a method to optimize the fermentation culture of ethanol wherein said method comprises a device that allows enough illumination of the fermentation culture with a source or sources of light that emit a precise light wavelength with low power energy consumption and low heat generation. In addition, the present invention provides said method where the source or sources of light, if necessary, can be switched on and off, or, when said sources of light have different light wavelengths, they can be switched on an off in alternated cycles. | 2009-12-10 |
20090305372 | Pre-treatment of biomass - The present invention relates to a method of pre-treatment of biomass, in order to improve the conversion of said biomass. The present invention also relates to a method of recovering chemicals used in said method. Further, the present invention relates to a method of converting pre-treated biomass into further products. | 2009-12-10 |
20090305373 | Trichoderma reesei glucoamylase and homologs thereof - The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications. | 2009-12-10 |
20090305374 | PROCESS FOR OBTAINING BIOCHEMICALS IN A ZERO-LIQUID DISCHARGE PLANT - A method is presented for the production of cellulosic ethanol, acetic acid and derivatives from the extract containing fibers and hemicelluloses after steam cooking of biomass in a host plant. The process is integrated with the host plant process to minimize the effect of loss of heat value from the extracted hemicelluloses and eliminate the need for the waste water treatment plant. | 2009-12-10 |
20090305375 | SUSTAINABLE SUPPLY OF BIOACTIVE MARINE PRODUCTS - The identification and characterization of the sources of terpenes from the soft corals | 2009-12-10 |
20090305376 | FERMENTER FOR PRODUCING BIOGAS FROM ORGANIC MATERIAL - The invention relates to a fermenter ( | 2009-12-10 |
20090305377 | Process and Device for Continuous Liquefaction of Organic Solids - A method for the continuous liquefying of organic solids in a fermenter, wherein an outwardly directed flow of solids is produced in a dammed-up liquid, the solids are added in the lower region of the fermenter and the solid fermentation residues are essentially collected and removed below the level of the dammed-up liquid. | 2009-12-10 |
20090305378 | INTEGRATED CHEMICAL PROCESS - A mineral carbonation process, characterised in that the silicate feedstock is thermally activated by using heat generated from the combustion of fuel prior to reacting the activated slurry feedstock with carbon dioxide. | 2009-12-10 |
20090305379 | DIGESTER SYSTEM - A manure mixture within an anaerobic digestion tank stratifies to form a liquid effluent layer and a sludge layer. Liquid effluent from the liquid effluent layer is withdrawn from the tank through a height adjustable valve. The height adjustable valve is adapted to automatically adjust the position of its intake end within the liquid effluent layer in response to the level of the sludge layer detected by a sludge meter located within the tank. Liquid effluent withdrawn from the tank is passed through a heat exchange system including at least one heat exchanger. Heat from the heat exchanger is transferred to the liquid effluent to produce heated liquid effluent. The heated liquid effluent is reintroduced back into the digestion tank such that the temperature of the manure mixture within the tank is maintained within a suitable temperature range for anaerobic digestion of the manure mixture. Additionally, the heated liquid effluent is sprayed in an upwards direction so as to effect mixing of the manure mixture within the tank. | 2009-12-10 |
20090305380 | ELECTROPORATION OF ADHERENT CELLS WITH AN ARRAY OF CLOSELY SPACED ELECTRODES - Adherent cells and other membranous structures that are immobilized on a solid surface are transfected by electroporation in which the electric field is produced by a array of closely spaced electrodes positioned above the surface. Each electrode is substantially smaller in at least one lateral dimension than the dimensions of a single cell, and the electrodes in each pair are spaced apart by distances selected such that that a maximum of one cell will reside within the field produced by each pair, and the distance of the electrodes above the surface to which the cells are adherent is small enough to place the cell within the resulting electric field and yet great enough to avoid contact of the electrodes with the cell membrane. | 2009-12-10 |
20090305381 | Activated Polymers Binding Biological Molecules - The present invention relates to activated polymer substrates capable of binding functional biological molecules, to polymer substrates comprising bound and functional biological molecules, to devices comprising such substrates and to methods of producing them. | 2009-12-10 |
20090305382 | ALKOXY INDOLINONE BASED PROTEIN KINASE INHIBITORS - Alkoxy indolinone based acid and amide derivatives have enhanced and unexpected drug properties as inhibitors of protein kinases and are useful in treating disorders related to abnormal protein kinase activities such as cancer. | 2009-12-10 |
20090305383 | Early Detection of Pathogens in Blood - The present invention is a method of extracting infectious pathogens from a volume of blood including the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The fibrin lysis reagent is preferably composed of plasminogen and streptokinase frozen in coincident relation until the fibrin lysis reagent is needed whereby streptokinase enzymatically reacts with plasminogen to form plasmin upon thawing. The plasminogen is suspended in an aqueous salt solution prior to freezing including NaCl and Na | 2009-12-10 |
20090305384 | Class II Human Histone Deacetylases, and Uses Related Thereto - The invention provides histone deacetylase class II nucleic acids and polypeptides, methods and reagents for their use, and related compounds including small molecule libraries containing class II histone deacetylase inhibitors. | 2009-12-10 |
20090305385 | Novel Cultured Silkworm Cells Capable of Highly Efficient Baculovirus Production and Protein Production - Known cell lines derived from silkworm exhibit low propagation efficiency of BmNPV. Accordingly, systems using known culture cell lines derived from silkworms take a long time to establish recombinant viruses, and are not suitable for preparation of virus solutions with high titers. The present invention provides a cell line Bme21 (FERM P-20852) that is derived from a silkworm embryo and is highly susceptible to BmNPV or its variant having the same biological characteristics. The present invention also provides a method of producing a recombinant virus, a method of producing a recombinant protein, a method of increasing efficiency of recombinant virus production, and a method of increasing efficiency of recombinant protein production, using the cell line Bme21 or its variant. | 2009-12-10 |
20090305386 | LOW OXYGEN BIOLOGICALLY MEDIATED NUTRIENT REMOVAL - The present invention is directed to a substantially odorless biologically mediated treatment process for solid and liquid organic wastes. The present invention also provides for a novel nutrient rich humus material produced from the biologically mediated treatment process. The bioconversion process of the present invention results from low electron acceptor concentrations and high quantities of microorganisms in a diverse microbial community. | 2009-12-10 |
20090305387 | COMPOSITIONS AND METHODS FOR CLEANING SURFACES - Cleaning compositions using the supernatant of lactic acid-producing bacteria are provided. Use of the compositions for disinfection/sanitization of surfaces are also disclosed. | 2009-12-10 |
20090305388 | METHOD FOR ELIMINATING CARBON DIOXIDE FROM WASTE GASES - A method for the elimination of carbon dioxide from waste gases includes the following steps. First, waste gases, which include carbon dioxide, are provided from a source for waste gases. Next, the waste gases are contacted with an absorbent composition that includes perfluorodecalin solution. The waste gases, especially the carbon dioxide, are then absorbed by the absorbent composition. The absorbent composition thereby absorbs the waste gases to eliminate the carbon dioxide. | 2009-12-10 |
20090305389 | PERMEABLE MEMBRANES IN FILM PHOTOBIOREACTORS - Embodiments of the present invention include photobioreactors with membranes to introduce carbon dioxide into media contained within film photobioreactors. Such membranes can also be used to remove dissolved oxygen from the media. In some embodiments, one or more membrane tubes are welded into a plastic film photobioreactor to make a one-piece reactor. According to some embodiments of the present invention, algae is grown in a photobioreactor using pressure, gas composition, and surface area along with sparging to control the pH in the photobioreactor. | 2009-12-10 |
20090305390 | METHOD AND INSTALLATION FOR PROCESSING WASTE AND PRODUCING METHANE - A method is provided for processing waste and producing methane, in which a chamber is filled with waste, in which it undergoes anaerobic degradation. According to the method, a large chamber and a small chamber are filled respectively with slightly organic waste and highly organic waste, and a liquid fraction generated by the degradation of the waste in the large chamber is introduced into the small chamber. Also provided is an apparatus adapted to implement such a method. | 2009-12-10 |
20090305391 | Triphasic bioreactor and process for gas effluent treatment - A triphasic bioreactor for physico-chemically treating a gas is disclosed. The triphasic bioreactor comprises a reaction chamber with a liquid and biocatalysts in suspension in the liquid, for catalyzing a reaction between the gas and the liquid to obtain a treated gas and a solution containing a reaction product. A gas bubbling means is provided in the reaction chamber for bubbling the gas to be treated into the liquid thereby dissolving the gas into the liquid and increasing a pressure inside the reaction chamber. The bioreactor further comprises a liquid inlet in fluid communication with the reaction chamber for receiving the liquid and filling the reaction chamber, a liquid outlet in fluid communication with the reaction chamber for releasing the solution and a gas outlet in fluid communication with the reaction chamber to release the treated gas. The bioreactor further comprises a retention device to retain the biocatalysts in the reaction chamber. The invention also concerns a process using the triphasic bioreactor. The triphasic bioreactor may advantageously be used for removing carbonic dioxide from a CO | 2009-12-10 |
20090305392 | DEVICE FOR PROCESSING SAMPLES - Device for sample processing, particularly sample conditioning as well as for the preparation and/or optionally for implementing a sequential process for an analyte from a biological sample, said device comprising a module for receiving and/or outputting at least one sample vessel or process vessel, a module for transporting a process vessel, a module for sample conditioning and a module for initiating a sequential process for an analyte. The modules are divided into at least two units that each possesses a control system, and which are connected through a first data bus. | 2009-12-10 |
20090305393 | Apparatus, System, and Method for in-Situ Measurements - The present invention relates to apparatus, systems and methods for performing in situ measurements, and, more particularly, to apparatus, systems and methods that can isolate a sample from a bulk fluid to measure characteristics of same without unwanted effects of perturbation in the bulk fluid. | 2009-12-10 |
20090305394 | DNA CHIP FOR DIAGNOSIS OF CORNEAL DYSTROPHY - The present invention relates to oligonucleotides for diagnosis of corneal dystrophy. More particularly, the present invention relates to oligonucleotides for detecting mutation of BIGH3 gene for diagnosis or corneal dystrophy including Avellino corneal dystrophy, which must be precisely diagnosed before vision correction surgery, and a DNA chip for diagnosis of corneal dystrophy, which has the oligonucleotides fixed thereon. According to the present invention, conventional microscopic diagnosis of corneal dystrophy can be replaced with a precise genetic method, which prevents a patient with corneal dystrophy from losing eyesight by eyesight correction surgery after erroneous diagnosis. | 2009-12-10 |
20090305395 | Reduction of the Hook Effect in Membrane-Based Assay Devices - A diagnostic test kit for detecting the presence or absence of a protease (e.g., aminopeptidase) within a test sample is provided. The test kit comprises a substrate that is capable of being cleaved in the presence of the protease to release a compound. The kit also comprises a lateral flow device that comprises a chromatographic medium. The chromatographic medium defines a detection zone within which is contained a first reagent (e.g., diazonium ion) that is capable of reacting with the compound to form a second reagent (e.g., aromatic azo compound). The second reagent exhibits a color that is different than the color of the first reagent. The lateral flow device also includes an absorbent material located adjacent to the chromatographic medium, the absorbent material receiving the test sample after flowing through the chromatographic medium. | 2009-12-10 |
20090305396 | Device for Culture of Cells or Micro-Organisms - This invention relates to a device intended for the culture of cells or micro-organisms, characterised by the fact that it comprises a chamber ( | 2009-12-10 |
20090305397 | Cellular entity maturation and transportation systems - A device for transporting at least one cellular entity during culture or maturation, the device comprising a substrate having one or more wells, said one or more wells being adapted to hold a cellular entity in a fluid, lid means for preventing entry or exit of the cellular entity from the one or more wells and fluid transport means connecting the one or more wells to enable flow of fluid or diffusion of chemical species. The apparatus alternatively or in addition comprise a module for transporting a payload at a controlled temperature, the module comprising an outer housing, an outer thermally insulating region, an inner thermally insulating region, and a heat sinking region located between the inner and outer thermally insulating regions, the inner thermally insulating region defining a cavity for receiving a payload, and heating means. | 2009-12-10 |
20090305398 | Recombinant Double-Stranded RNA Phage, and Use of the Same - A recombinant double stranded RNA (dsRNA) phage expresses dsRNA-encoded genes in eukaryote cells. Recombinant dsRNA phage are useful for the expression of dsRNA expression cassettes encoding passenger genes, such as, but not restricted to, vaccine antigens, bioactive proteins, immunoregulatory proteins, antisense RNAs, and catalytic RNAs in eukaryotic cells or tissues. Methods are provided to deliver recombinant dsRNA phage to eukaryotic cells and tissues, either by direct administration, formulated in lipid or polylactide-coglycolide, or by utilizing a bacterial vaccine vector. | 2009-12-10 |
20090305399 | DNA encoding OSK1 toxin peptide analogs and vectors and cells for combinant expression - Disclosed is a composition of matter comprising an OSK1 peptide analog, and in some embodiments, a pharmaceutically acceptable salt thereof. A pharmaceutical composition comprises the composition and a pharmaceutically acceptable carrier. Also disclosed are DNAs encoding the inventive composition of matter, an expression vector comprising the DNA, and host cells comprising the expression vector. Methods of treating an autoimmune disorder and of preventing or mitigating a relapse of a symptom of multiple sclerosis are also disclosed. | 2009-12-10 |
20090305400 | Modified chaperonin 10 - A novel granule dispersion composition which comprises water and, dispersed therein, a finely pulverized, sparingly water-soluble substance. The granule dispersion composition is prepared by dispersing in water a granular material which comprises a specific substance sparingly soluble in water, a polymer, and an oil and has an average particle diameter of 1 μm or smaller and in which the ratio of the total weight of the polymer and oil to the weight of the specific substance is 1.5 or higher. | 2009-12-10 |
20090305401 | PLASMA-FREE PLATELET LYSATE FOR USE AS A SUPPLEMENT IN CELL CULTURES AND FOR THE PREPARATION OF CELL THERAPEUTICS - The present invention provides a cell culture medium supplement comprising plasma-free platelet lysate and medium supplemented with this supplement. The present invention further provides a method for preparing the supplement comprising the steps of (a) preparing platelet rich plasma; (b) removing the plasma; and (c) lysing the platelets. | 2009-12-10 |
20090305402 | Methods and compositions for using zinc finger endonucleases to enhance homologous recombination - Described herein are methods of generating a genetically modified cell by providing a zinc finger endonuclease (ZFE) that includes an endonuclease domain that cuts DNA, and a zinc finger domain that includes a plurality of zinc fingers that bind to a specific nucleotide sequence within the endogenous chromosomal target DNA in the primary cell. Further, the methods can include contacting the endogenous chromosomal target DNA sequence with the zinc finger endonuclease in the primary cell such that the zinc finger endonuclease cuts both strands of a nucleotide sequence within the endogenous chromosomal target DNA sequence in the primary cell, thereby enhancing the frequency of homologous recombination in the endogenous chromosomal target DNA sequence. | 2009-12-10 |
20090305403 | Isolation and Differentiation of Adult Hippocampal Arctic Squirrel Neural Stem Cells - Neuronal stem cell lines derived from the Arctic Ground Squirrel, methods related to culturing and maintaining a neuronal stem cell line derived from the Arctic Ground Squirrel and a culture media required to maintain and differentiate a neuronal stem cell line derived from the Arctic Ground Squirrel is disclosed. Antibodies specific for antigens expressed on a neuronal stem cell line derived from the Arctic Ground Squirrel, and products and methods related to the use of neuronal stem cell lines derived from the Arctic Ground Squirrel are also included. | 2009-12-10 |
20090305404 | Methods and compositions relating to blastomere-derived human embryonic stem cells - The invention provides methods for producing human embryonic stem cells from blastomeres with reduced or no animal cells or products, including no serum regardless of source and including xeno-free conditions, without compromising derivation efficiency. | 2009-12-10 |
20090305405 | USE OF TGF BETA SUPERFAMILY ANTAGONISTS AND NEUROTROPHINS TO MAKE NEURONS FROM EMBRYONIC STEM CELLS - This invention provides a system for efficiently producing differentiated cells from pluripotent cells, such as human embryonic stem cells. Rather than permitting the cells to form embryoid bodies according to established techniques, differentiation is effected directly in monolayer culture on a suitable solid surface. The cells are either plated directly onto a differentiation-promoting surface, or grown initially on the solid surface in the absence of feeder cells and then exchanged into a medium that assists in the differentiation process. The solid surface and the culture medium can be chosen to direct differentiation down a particular pathway, generating a cell population that is remarkably uniform. The methodology is well adapted to bulk production of committed precursor and terminally differentiated cells for use in drug screening or regenerative medicine. | 2009-12-10 |
20090305406 | Method of cultivation of human mesenchymal stem cells, particularly for the treatment of non-healing fractures, and bioreactor for carrying out this cultivation method - The invention relates to a novel method of cultivation of mesenchymal stem cells, wherein after aseptic separation of mononuclear cells from the marrow blood, said cells are seeded in low density into sterile plastic cultivation vessels and cultivated for approximately one to three weeks in CellGro™ Hematopoietic Stem Cell Medium, certified for the clinical use, with an addition of 10% human serum and supplements, wherein the supplements are added at least once in the course of the cultivation, without removal of hematopoietic cells and without medium exchange during the cultivation procedure, without any interference with the closed cultivation system, under the standard conditions for the cultivation of tissue cultures. | 2009-12-10 |
20090305407 | Devices and Methods for Sampling Biological Fluids - Devices, instruments, systems and methods are provided in which a primary line that receives a biological fluid is selectively sampled by a plurality of collection chambers. Selective sampling occurs by selectively accessing the primary line and selective engagement of a sampling pump under control of a microprocessor. Further, the instrument housing reversibly houses a drive assembly and sample collection housing to permit the interchangeability of drive assemblies and collection housings and thus enhance sterility or reduction of cost. | 2009-12-10 |
20090305408 | Method for the rapid expansion of antigen specific t-cell - The present invention encompasses a method for expanding an antigen specific T cell from a population of cells. The method of the present invention comprises contacting a population of cells with an MHC restricted antigenic peptide, a cytokine and a co-stimulatory signal. The invention also encompasses compositions and kits comprising an antigen specific T cell. | 2009-12-10 |
20090305409 | Liposome Capable of Effective Delivery of Given Substance Into Nucleus - Disclosed is a non-viral vector capable of delivering a given substance into the nucleus of a target cell effectively even when the target cells is a undividable cell such as a dendritic cell. A bilamellar liposome having a first lipid membrane and a second lipid membrane successively from the outside, the first lipid membrane having a membrane-fusing ability and the second lipid membrane having on its surface a nuclear transport peptide. | 2009-12-10 |
20090305410 | FLUORESCENT COMPOUNDS - The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions. | 2009-12-10 |
20090305411 | RECOMBINANT ANTIBODIES AND IMMUNOCONJUGATES TARGETED TO CD-22 BEARING CELLS AND TUMORS - Methods and compositions relating to anti-CD22 antibodies with high binding affinity, and immunoconjugates comprising the anti-CD22 antibody linked to a therapeutic agent such as a | 2009-12-10 |
20090305412 | MECHANICALLY REVERSIBLE GEL - The present invention relates to a process for making a gel comprising combining a silanol species comprising at least two silanol groups per molecule and a hydrophilic hydroxyl species comprising at least two hydroxyl groups per molecule. The gel is capable of being converted to a liquid by application of a mechanical shear force and the liquid is capable of being converted to the gel in the absence of said mechanical shear force. | 2009-12-10 |
20090305413 | MULTIPOTENT ADULT STEM CELLS HAVING AN ABILITY OF OCT4 EXPRESSION DERIVED FROM UMBILICAL CORD BLOOD AND METHOD FOR PREPARING THE SAME - The present invention relates to multipotent adult stem cells expressing Oct4, derived from umbilical cord blood (UCB) and also these cell are expressing CD29, CD31, CD44, simultaneously, a method for preparing the same, and more specifically to multipotent adult stem cells which are obtained by culturing umbilical cord blood-derived monocytes in a medium containing bFGF (basic fibroblast growth factor) and human serum or plasma. In addition, multipotent adult stem cells expressing Oct-4 from UCB are morphologically spindle or round shaped cells Although the stem cells according to the present invention are adult stem cells, they are multipotent and capable of differentiating into ectodermal-, messodermal-, endodermal-originated tissue or cells including osteogenic cells or nerve cells etc, thus they can be effectively used in the treatment of intractable diseases and incurable diseases. | 2009-12-10 |
20090305414 | Methods Of Tissue Engineering - An improved substrate for growing mono-layers of adherent-type cells and methods of growing tissue structures, ex vivo. The improved substrate, which comprises a silicon substrate coated with a photo cleavable polymer, releases adherent cells non-enzymatically. Also disclosed are methods for assembling complex layers of cells of various types. | 2009-12-10 |
20090305415 | Method for preserving proliferation and differentiation potential of undifferentiated cells - A method for preserving proliferation and differentiation potential of undifferentiated cells, has steps of providing a culture carrier having a surface coated with a biological material selected from the group consisting of polysaccharide, sulfated polysaccharide and derivatives thereof; and inoculating and culturing the undifferentiated cells on the surface in the culture carrier with an appropriate medium, such that the proliferation and differentiation potential of undifferentiated cells are preserved. The method can be used for expanding stem cells in vitro without loss of their replicative ability and differentiation capacity. Therefore, the method according to the present invention is amenable to application in regenerative medicine, tissue engineering, and therapy using umbilical cord blood and other cell sources such as peripheral blood, stem cells, tissue progenitor cells, and tissue cells. | 2009-12-10 |
20090305416 | Method for regulating proliferation of cells - A method for regulating proliferation of cells, has steps of providing a first culture system with a surface that is coated with a biological material; inoculating and culturing cells on the surface of the first culture system in an appropriate medium, such that the proliferation of the cells is preserved; collecting the cells; providing a second culture system with a surface; and inoculating and culturing the cells on the surface of the second culture system in a culture medium containing the biological material, such that the proliferation of the cells is promoted. A method for regulating proliferation of cells is also provided, the method being the same as the previous method except that the step of inoculating and culturing in a first culture system is performed before the step of inoculating and culturing in a second culture system. | 2009-12-10 |
20090305417 | CELL CULTURE - A cell culture surface comprising a substrate and a polymer comprising a carboxylic acid monomer, wherein the carboxylic acid concentration of the polymer is from | 2009-12-10 |