| 44th week of 2009 patent applcation highlights (10-29-09/10-29-09_40) part 40 |
| Patent application number | Title | Published |
|---|
| 20090269726 | Method of teaching emergent readers - A method and apparatus are provided for teaching emergent readers. The method includes the steps of associating a first color of with all short vowel combinations; | 10-29-2009 |
| 20090269727 | NETWORK APPARATUS, SYSTEM AND METHOD FOR TEACHING MATH - A network for teaching mathematics having a teacher device for forming a mathematical query networked to a student device for receiving the input data from a student on a network is disclosed. The student device on the network may use a numerical key pad for entering mathematical solutions and a display for seeing the input. Additionally, a method for networking students and teachers for mathematics comprising forming a mathematical query on a teacher device and receiving data from a student device related to the formed query over a network with the teacher device is disclosed. | 10-29-2009 |
| 20090269728 | ATHLETE TRAINING SYSTEM - A system for training an athlete during a training period includes a digital system that is programmed to: receive a plurality of inputs regarding a current state of the athlete and a training goal for the athlete; employ an expert system to generate a training prescription for the athlete; and generate a plurality of control outputs that correspond to the listing of training activities. An exercise apparatus includes at least one activity device that is configured to: facilitate the athlete performing a predetermined exercise; receive the control output from the digital system and to adjust an exercise parameter so as to correspond to the training parameter indicated by the control output; and generate an electronic result output indicative of use by the athlete of the activity device wherein the result output is transmitted to the digital system. | 10-29-2009 |
| 20090269729 | SYSTEM FOR TRACKING THE PASSAGE OF TIME - Systems and methods for tracking the passage of time are described. One system includes a predetermined event and a set of counting units, with each counting unit having a first side displaying a number. Each number corresponds to a number of time increments until the predetermined event. Each counting unit has a second side displaying a portion of an image corresponding with the predetermined event, and when the counting units are arranged in a predetermined arrangement the image is produced. | 10-29-2009 |
| 20090269730 | SIMULATION AUTHORING TOOL - A computer program for creating a social simulation comprises code segments for enabling a user to create a plurality of social simulation stages, to create a map associating each of the plurality of stages with at least one other of the plurality of stages, and to assign a first score value to a first response and a second score value to a second response, wherein the first response and the second response are received from a student during at least one of the plurality of social simulation stages. The computer program further enables the user to assign a condition to a conditional stage of the plurality of simulation stages such that the student experiences the conditional stage only if a cumulative score corresponds to a target score, wherein the cumulative score includes the first score value and the second score value. | 10-29-2009 |
| 20090269731 | E3-INDEPENDENT UBIQUITINYLATION ASSAY - Disclosed herein are compositions and methods for assaying ubiquitination independent of ubiquitin ligase (E3) or a target protein. | 10-29-2009 |
| 20090269732 | Methods for Diagnosing Oncogenic Human Papillomavirus (HPV) - Methods for diagnosis of HPV infection in a subject are provided. HPV infection in a subject can be determined by generating mass profile data for a biological sample from the subject and correlating the mass profile data with reference mass profiles to detect the presence or absence, and/or quantity of at least one biomarker associated with HPV infection. Methods for detecting at least one biomarker associated with HPV infection in a biological sample are also provided. | 10-29-2009 |
| 20090269733 | Devices and Methods for the Rapid Analysis of Pathogens in Biological Fluids - The present invention relates to devices and methods for rapidly determining whether a biological fluid contains a suspect Gram positive bacterial, a Gram negative bacterial or a viral pathogen. The invention particularly pertains to such devices and methods wherein the biological fluid is cerebrospinal fluid, and wherein the suspect pathogen is a causative agent of meningitis. | 10-29-2009 |
| 20090269734 | METHOD FOR DETERMINATION OF RECOGNITION SPECIFICITY OF VIRUS FOR RECEPTOR SUGAR CHAIN - A method in which the recognition specificity of a virus for a receptor sugar chain can be easily determined with a simple instrument or apparatus is provided. In a method for determining the recognition specificity of a virus for a receptor sugar chain or for determining the change in a host infected in accordance with the mutation of virus comprising, a sample of the virus is brought into contact with a support having a polymer with sialo-oligosaccharide immobilized on the surface thereof; and the degree of binding therein is assayed to determine the recognition specificity of said virus for said receptor sugar chain and to determine the change in a host range. The method is suitable for the surveillance of virus. | 10-29-2009 |
| 20090269735 | SAMPLE PRETREATMENT SOLUTION FOR IMMUNOLOGICAL TEST AND METHOD FOR USING THE SAME - Sample pretreatment solutions for influenza virus tests by immunochromatography are described. | 10-29-2009 |
| 20090269736 | Prognostic markers for prediction of treatment response and/or survival of breast cell proliferative disorder patients - Aspects of the present invention provide compositions and methods for prognosis of, and/or predicting the estrogen treatment outcome of breast cell proliferative disorder patients, and in particular of patients with breast carcinoma. In preferred embodiments, this is achieved, at least in part, by determining the expression level of PITX2, and/or the genetic or the epigenetic modifications of the genomic DNA associated with the gene PITX2. Additional aspects of the invention provide novel sequences, oligomers (e.g., oligonucleotides or peptide nucleic acid (PNA)-oligomers), and antibodies, which have substantial utility in the described inventive methods and compositions. | 10-29-2009 |
| 20090269737 | Integrated non-homogeneous nucleic acid amplification and detection - The present invention relates to an integrated method of amplifying and analyzing target nucleic acids, in which immobilized or immobilizable oligonucleotide capture probes are provided and a nucleic acid containing sample to be analyzed is added together with a reagent mixture, which mixture contains all reagents needed for amplification and subsequent analysis of said target nucleic acids. In the method amplification of the target nucleic acids, hybridization of said amplified target nucleic acids to the capture probes and separating the hybrids formed from un-reacted components, as well as the detection and measuring of the amount of labeled, hybridized target nucleic acids by means of a detectable signal, is performed in one reaction chamber. Further provided are reagent mixtures and kits for use in such methods. | 10-29-2009 |
| 20090269738 | Method of Screening for the Presence of a Genetic Defect Associated With Deep Venous Thrombosis - The present invention relates to a method for screening an individual for the presence in his/her genome of a genetic marker that is indicative of an increased risk of deep venous thrombosis, wherein the genetic marker is haplotype 2 of the fibrinogen γ gene (FGG-H2) as given in FIG. | 10-29-2009 |
| 20090269739 | Kit for detection of telomerase reverse transcriptase nucleic acids - The invention provides compositions and methods related to human telomerase reverse transcriptase (hTRT), the catalytic protein subunit of human telomerase. The polynucleotides and polypeptides of the invention are useful for diagnosis, prognosis and treatment of human diseases, for changing the proliferative capacity of cells and organisms, and for identification and screening of compounds and treatments useful for treatment of diseases such as cancers. | 10-29-2009 |
| 20090269740 | Pancreatic Cancer Genes - The present invention provides the art with the DNA coding sequences of polynucleotides that are up-or-down-regulated in cancer and dysplasia. These polynucleotides and encoded proteins or polypeptides can be used in the diagnosis or identification of cancer and dysplasia. Inhibitors of the up-regulated polynucleotides and proteins can decrease the abnormality of cancer and dysplasia. Enhancing the expression of down-regulated polynucleotides or introducing down-regulated proteins to cells can decrease the growth and/or abnormal characteristics of cancer and dysplasia. | 10-29-2009 |
| 20090269741 | Method for assessing traits selected from longissimus dorsi peak force, intramuscular fat, retail beef yield and net feed intake in bovine animals - A method for assessing a trait in a bovine animal selected from the group consisting of longissimus dorsi peak force, intramuscular fat, retail beef yield and net feed intake, comprising the steps of: (1) providing a nucleic acid from the bovine animal or carcass; (2) assaying for the occurrence of a single nucleotide polymorphism (SNP), wherein the identification of said nucleotide occurrence as set forth herein is associated with variation in longissimus dorsi peak force, intramuscular fat deposition, retail beef yield or net feed intake. | 10-29-2009 |
| 20090269742 | SUBSTRATE FOR IMMOBILIZING BIOPOLYMER AND METHOD OF IMMOBILIZING BIOPOLYMER BY USING THE SAME - [Problems] To immobilize a chain-type biopolymer in an elongated state at a predetermined position on a substrate. | 10-29-2009 |
| 20090269743 | DNA Collection Sticker and Method for Isolating DNA From the Sticker - The present invention relates a sticker for DNA collection and a method for isolating DNAs using the same. Particularly, the sticker for DNA collection is covered with a paint solution comprising EDTA, Tris, SDS and peyonine to isolate keratins exclusively when attached onto human skin and detached. Further, the specific sticker for DNA collection separates DNAs efficiently to amplify genes by using a PCR technique. Therefore, the present invention can be applied to identify a real child and investigate a crime with a fingerprint and to screen genetic diseases. | 10-29-2009 |
| 20090269744 | CANCER DETECTION METHOD - The present application concerns methods and compositions which can be used to detect cancer in mammals, in particular in humans. It notably describes serum markers of cancers and their uses in diagnosis methods. It also concerns tools and/or kits which can be used to implement these methods (reagents, probes, primers, antibodies, chips, cells, etc.), their preparation and their uses. The invention can be used to detect the presence or the progression of a cancer, particularly breast cancer, including at an early stage. | 10-29-2009 |
| 20090269745 | RNA EXTRACTION METHOD AND RNA DETECTION METHOD - The present invention provides a method for inactivating RNase which generally presents in a sample such as biological sample (especially an excrement sample), or in a sample such as a living body-derived sample (especially an excrement-derived sample) obtained by separation of an RNA-including body therefrom or the like; a method for extracting and detecting RNA from the sample. An RNA extraction method, comprising the steps of: obtaining a mixture under a heating condition, said mixture comprising: a sample comprising an RNA-including body and RNase, and an alkaline treating reagent comprising at least a reducing agent, and having pH of 8.1 or higher, and conducting inactivation of the RNase and extraction of RNA from the RNA-including body by keeping the mixture under the heating condition. An RNA detection method, comprising conducting RNA amplification reaction by mixing a treated sample liquid comprising RNA extracted by the extraction method and an amplification reaction solution. | 10-29-2009 |
| 20090269746 | MICROSEQUENCER-WHOLE GENOME SEQUENCER - The method and apparatus are disclosed to support speedy sequencing of genomes of individuals. The method comprises random digestion of a stretch of DNA; adaptor ligation of adaptor DNA fragments to DNA segments produced in random digestion, each said adaptor DNA fragment containing a sequence which is complementary to a single DNA primer; PCR amplification of the ligated segments produced in adaptor ligation, utilizing a single DNA primer; distributing the ligated segments into one or more pre-defined isolated locations of a sequencing apparatus, each said location containing DNA fragments placed there for capturing a unique kind of digested DNA segments; capturing at each location a unique kind of amplified DNA segments by hybridization with the DNA fragments, dislodging captured DNA segments from DNA fragments; adding DNA sequencing reaction components into the locations; performing sequencing reactions at each location; separating the products of the sequencing reactions in the sequencing apparatus; and determining the sequences of DNA segments captured at individual locations of the sequencing apparatus. The apparatus comprises one or more isolated locations, each location has a reservoir containing DNA fragments placed there for capturing a unique kind of DNA segments from a DNA solution after dispensing the DNA solution into the reservoir; one or more channels performing DNA separation according to size, said channels being associated with one or more reservoirs; one or more gates controlling the flow of substances in the reservoirs; an optical system which induces fluorescence excitation in, and detects fluorescence emission in the channels; and a computer to produce DNA sequence data. | 10-29-2009 |
| 20090269747 | Marker Genes Based on Amiodarone Treatment for Screening of Drug Inducing Toxicity and Screening Method Therefor - The present invention relates to a marker gene for screening of drug candidates inducing pulmonary toxicity and a screening method using the same, more precisely a marker gene up- or down regulated by amiodarone which is a drug inducing pulmonary toxicity and a method for screening drug candidates inducing pulmonary toxicity using the same. The marker gene of the present invention can be effectively used for monitoring and identifying drugs or chemical having high risk of inducing pulmonary toxicity and can be used as an effective tool for examining the mechanism of amiodarone which causes pulmonary toxicity and side effects. | 10-29-2009 |
| 20090269748 | IDENTIFICATION OF SUBSTANCES THAT INHIBIT NEMO OLIGOMERIZATION - The present invention provides methods for screening for substances which inhibit the oligomerization of NEMO and/or IKK-related complexes and/or signaling pathways based on the interference with NEMO oligomerization | 10-29-2009 |
| 20090269749 | METHOD FOR HIGH-THROUGHPUT AFLP-BASED POLYMORPHISM DETECTION - The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-) dominant genotypes of the genetic markers. | 10-29-2009 |
| 20090269750 | MARKER AND METHOD FOR CANCER DIAGNOSIS - The present invention relates to a diagnostic cancer marker using variation of a granulocyte colony stimulating factor (G-CSF) gene and a method for preparing the same, and more specifically, relates to a method for diagnosing cancer and/or assessing the state of cancer progression using an oligonucleotide having the 3′-terminal end of exon 2 region linked to the 5′-terminal end of exon 4 region of a G-CSF gene as a diagnostic cancer marker. According to the present invention, cancer can be quickly and exactly diagnosed using variation in a G-CSF gene expression. | 10-29-2009 |
| 20090269751 | DOT1 HISTONE METHYLTRANSFERASES AS A TARGET FOR IDENTIFYING THERAPEUTIC AGENTS FOR LEUKEMIA - The present invention provides polypeptides with histone H3 lysine 79 methyltransferase activity as well as nucleic acids encoding the same. Also provided are methods of using the polypeptides and nucleic acids of the invention in screening assays to identify compounds of interest. Further provided are diagnostic methods for leukemia and prognostic methods to predict the course of the disease in a subject. | 10-29-2009 |
| 20090269752 | METHOD FOR SELECTING NUCLEIC ACIDS THAT BOND WITH HIGH-AFFINITY TO A TARGET - The invention relates to a method for selecting nucleic acids that bond with high affinity to a target molecule from a mixture of nucleic acids, comprising the following steps: a) loading a column with the target molecules whereby the target molecules are immobilized in said column, b) feeding the mixture of nucleic acids into a first end of the column, to create a defined volumetric flow of medium through the column, running from the first end to the second end of said column, c) immobilizing the nucleic acids to the target molecule wherein an affinity of the nucleic acids to the target molecule decreases as the distance from the first end of the column increases, d) stopping the volumetric flow of medium through the column after a defined period of time, e) cutting the column into column segments, and allocating a routing co-ordinate to each segment, and f) identifying and collecting nucleic acids that bond with a high affinity to the target molecule by desorbing the immobilized nucleic acids from at least one segment in a non-specific manner and extracting the nucleic acids, wherein the routing co-ordinate allocated a segment in step e) is allocated to the nucleic acids desorbed from that segment. | 10-29-2009 |
| 20090269753 | GENOTYPING FOR SRC-1 PREDICTS FOR BONE LOSS - Osteoporosis is a common skeletal disease characterized by loss of bone mineral density (BMD) and increased risk of fracture. Osteoporosis most commonly occurs in postmenopausal women due to estrogen deficiency. We identified 3 genetic variants in steroid receptor coactivator 1 (SRC-1) that are significantly associated with a decrease in BMD in women. We characterized a functional variant in exon 18 of SRC-1 that is associated with increased loss of bone mineral density in women who received tamoxifen for treatment or prevention of breast cancer. In vitro experiments show that this variant decreases estrogen receptor alpha response (ER-alpha) to hormone, suggesting an attenuated response to endogenous and exogenous hormones in the bone of these women, and therefore a need for additional bone protective measures. | 10-29-2009 |
| 20090269754 | METHOD OF PRODUCING AMPLIFICATION PRODUCT BY PCR AND USAGE THEREOF - A method of producing a PCR amplification product is provided that suppresses an effect of precipitate, turbidity, or the like derived from a whole blood sample on a detection in the detection of an amplified nucleic acid by an optical unit. The amplification product complementary to a target nucleic acid in the whole blood sample is produced by PCR in a condition where a ratio of the whole blood sample in a PCR reaction solution is in the range of 0.1 to 0.9% by volume or 0.01 to 1.8 g/L in term of hemoglobin content. When the PCR is carried out with such conditions, even with an untreated whole blood sample, a monitoring of the amplification product by the optical unit can be done while suppressing the effect of the precipitate or the turbidity. | 10-29-2009 |
| 20090269755 | MEANS AND METHOD FOR INDUCING EXON-SKIPPING - In the present invention means and method are provided for optimising exon-skipping using exon-internal AON. We show that skipping efficiencies are improved by targeting putative splicing regulatory sequences (ESEs) within an exon. Such double targeting may be particularly useful for exons with which efficient skipping was difficult to obtain prior to the invention. | 10-29-2009 |
| 20090269756 | PRIMER SET FOR AMPLIFYING CYP2C19 GENE, REAGENT FOR AMPLIFYING CYP2C19 GENE CONTAINING THE SAME, AND THE USES THEREOF - Primer sets for amplifying target regions containing sites to be detected in the CYP2C19 gene by a gene amplification method are provided, wherein the primer sets can amplify the regions specifically. Two pairs of primer sets are used including forward primers consisting of the base sequences of SEQ ID NOs: 12 and 32 as well as reverse primers consisting of the base sequences of SEQ ID NOs: 22 and 48, respectively. The use of these primer sets makes it possible to amplify two target regions including parts where two types of polymorphisms (CYP2C19*2 and CYP2C19*3) of the CYP2C19 gene are generated, respectively, in the same reaction solution at the same time. | 10-29-2009 |
| 20090269757 | DIAGNOSIS KITS AND METHOD FOR DETECTING CANCER USING POLYMORPHIC MINISATELLITE - The present invention relates to a kit and a method for diagnosing cancer using polymorphic minisatellites (MS), more specifically, relates to a primer set for detecting polymorphic minisatellites MUC2-MS6 or MUC2-MS7 in the MUC2 gene, a DNA typing kit comprising said primer set, and a kit and a method for diagnosing cancer using a primer set for detecting polymorphic minisatellites MUC2-MS6, MUC2-MS7 or hTERT-VNTR 2-2. According to the present invention, DNA typing of MUC2-MS6 and MUC2-MS7 can effectively achieve the parentage identification, kinship identification or medicolegal examination, because the polymorphic minisatellites MUC2-MS6 and MUC2-MS7 are inherited through meiosis according to Mendelian genetics. In addition, the polymorphic minisatellites MUC2-MS6, MUC2-MS7 and hTERT-VNTR 2-2 can be used to predict and diagnose various cancers; such as gastric cancer, colon cancer, rectal cancer and prostate cancer etc. | 10-29-2009 |
| 20090269758 | Diagnostic methods and kits for functional disorders - The present invention relates to methods for the diagnosis of functional disorders in humans. A method of the invention, in certain embodiments, comprises the detection of one or more polymorphisms in mitochondrial DNA of a human. The current invention further provides kits for use in a method of the invention. | 10-29-2009 |
| 20090269759 | UNNATURAL POLYMERASE SUBSTRATES THAT CAN SUSTAIN ENZYMATIC SYNTHESIS OF DOUBLE STRANDED NUCLEIC ACIDS FROM A NUCLEIC ACID TEMPLATE AND METHODS OF USE - Nucleotide analogs that can sustain the enzymatic synthesis of double-stranded nucleic acid from a nucleic template are described. The nucleotide analogs include: (i) a base selected from the group consisting of adenine, guanine, cytosine, thymine, uracil and their analogs; (ii) a label attached to the base or analog of the base via a cleavable linker; (iii) a deoxyribose; and (iv) one or more phosphate groups. The linker and/or the label inhibits template directed polymerase incorporation of a further nucleotide substrate onto an extended primer strand. In addition, cleavage of the linker leaves a residue attached to the base which is not present in the natural nucleotide and which does not inhibit extension of the primer strand. The nucleotide analogs can therefore be used as reversible terminators in sequencing by synthesis methods without blocking the 3′ hydroxyl group. Methods of sequencing DNA using the substrates are also described. | 10-29-2009 |
| 20090269760 | ENRICHMENT METHODS FOR THE DETECTION OF PATHOGENS AND OTHER MICROBES - The present invention provides novel enrichment, testing and detection methods for detection of pathogens or other microbes in a food, water, wastewater, industrial, pharmaceutical, botanical, environmental samples and other types of samples analyzed by enrichment-detection methods. In preferred aspects, a sample is obtained at a first location and is diluted (e.g., in the case of a solid or semi-solid sample or liquid) at the first location at a ratio of about 1:0 (wt./vol.) to 1:2 (wt./vol.), or greater, preferably at a ratio of about 1:0.1 (wt./vol.) or greater, or more preferably, at a ratio of about 1:2 (wt./vol.) or greater. The diluted sample is incubated at an optimal temperature in an incubator and either tested locally, or sent in a shipping incubator to a second location that is a remote test location. The incubated sample is received and tested at the second location by assaying the sample, or a portion thereof, with an assay suitable to detect the pathogen or other microbe. In alternate embodiments, no dilution at the first location is required, and optionally minimal additions to adjust intrinsic deficiencies may be made, but the sample is nonetheless incubated during transit to the test location. | 10-29-2009 |
| 20090269761 | Genetic markers associated with age-related macular degeneration, methods of detection and uses thereof - Disclosed is a method for identifying an individual who has an altered risk for developing age related macular degeneration comprising detecting a single nucleotide polymorphism (SNP) | 10-29-2009 |
| 20090269762 | Cotton event PV-GHGT07(1445) and compositions and methods for detection thereof - The present invention provides DNA compositions and assays for detecting the presence of the DNA compositions in PV-GHGT07(1445) cotton event based on the DNA sequence of the recombinant construct inserted into the cotton genome and of the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided. | 10-29-2009 |
| 20090269763 | Reprogramming a cell by inducing a pluripotent gene through RNA interference - The invention relate to methods, compositions, and kits for reprogramming a cell. In one embodiment, the invention relates to a method for inducing the expression of at least one gene that contributes to a cell being pluripotent or multipotent. In yet another embodiment, the method comprises inhibiting the expression of a gene that codes for a protein involved in transcriptional repression. In yet another embodiment, the invention relates to a reprogrammed cell or an enriched population of reprogrammed cells that can have characteristics of an ES-like cell, which can be re- or trans-differentiated into a differentiated cell type. | 10-29-2009 |
| 20090269764 | COMPOSITIONS AND METHODS FOR DETECTION OF PROPIONIBACTERIUM ACNES NUCLEIC ACID - Methods for amplifying and detecting | 10-29-2009 |
| 20090269765 | Compositions And Methods For Detection Of Small Molecules Using Dyes Derivatized with Analyte Responsive Receptors in a Chemiluminescent Assay - Compositions, methods, and systems for detecting small molecules using chemiluminescent signaling assay technology are provided. One system provided herein comprises a chromophore; an oxalate ester, a peroxide, and a modulating agent, wherein the modulating agent will perturb a chemiluminescent signal generated by an interaction among the chromophore, the oxalate ester, and a peroxide; and the perturbation will occur in response to an analyte. One method provided herein comprises combining a chromophore, an oxalate ester, a peroxide, and a modulating agent, wherein: the modulating agent will perturb a chemiluminescent signal generate by an interaction among the chromophore, the oxalate ester, and a peroxide; and the perturbation will occur in response to an analyte. Another method provides a calorimetric or fluorometric signal response in the presence of an analyte. | 10-29-2009 |
| 20090269766 | NUCLEIC ACID AMPLIFICATION IN THE PRESENCE OF MODIFIED RANDOMERS - The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR. | 10-29-2009 |
| 20090269767 | MICROFLUIDIC CHIP DEVICES AND THEIR USE - A microfluidic chip device (MCD) and its use for performing miniaturized assays on magnetic microbeads (MMs) are described. The MCD is particularly useful for carrying out miniaturized transcript analysis by aiding affinity capturing (TRAC) assays, including PCR. The MCD comprises at least one reaction chamber with sealable liquid connections and at least one fluidic pillar filter in each chamber. The fluidic pillar filter comprises rods with spacings allowing MMs to pass. The sealable liquid connections feed liquid to the reaction chamber, wherein air bubbles are removed. The liquid stream contacts the MMs, which are manipulated with a magnetic rod. The liquid connections enable trapping of the MMs behind the pillar filters or in the channel, while the liquid is changed. | 10-29-2009 |
| 20090269768 | DETECTION OF HIGH GRADE DYSPLASIA IN CERVICAL CELLS - Methods of using probes and probe sets for the detection of high grade dysplasia and carcinoma in cervical cells are described. Methods of the invention include hybridizing one or more chromosomal probes to a biological sample obtained from a subject and detecting the hybridization pattern of the chromosomal probes to the sample to determine whether the subject has high grade dysplasia or carcinoma. Methods of the invention also include preliminary screening the cells for a marker associated with a risk for cancer, and preferably involves screening for HPV infected cells by in situ hybridization using an HPV probe mixture. | 10-29-2009 |
| 20090269769 | Drug Discovery Methods Involving A Preclinical, In Vitro Isolated Gastrointestinal Epithelial Stem Cell-Like Progenitor Cell System - The described invention relate to systems comprising isolated human gastrointestinal segment-specific epithelial stem cell-like progenitor cells and uses thereof in drug discovery. | 10-29-2009 |
| 20090269770 | METHODS FOR EVALUATION PROGNOSIS AND FOLLOW-UP OF DRUG TREATMENT OF PSYCHIATRIC DISEASES OR DISORDERS - The present invention provides methods for evaluating the pharmacological efficacy of drugs or drug candidates in treatment of psychiatric diseases or disorders, particularly schizophrenia, and for predicting the efficacy of drugs or drug combinations indicated for treatment of both positive and negative symptoms of psychiatric diseases or disorders in an individual having such a disease or disorder. In both methods, the drugs or drug candidates evaluated are assessed for their ability to produce certain changes in the expression of specific genes in peripheral mononuclear cells in blood of psychiatric patients, which are similar to the changes obtained following treatments with reference drugs or drug combinations effective against both positive and negative symptoms of psychiatric diseases or disorders. | 10-29-2009 |
| 20090269771 | METHOD OF SEQUENCING AND MAPPING TARGET NUCLEIC ACIDS - The present teachings pertain to methods, compositions, reaction mixtures, and kits for mapping a low complexity sequence to a locus in a genome. In some embodiments, the low complexity sequence can be used to determine the methylation profile of a target nucleic acid. A strand-replacing reaction results in a product containing a first strand and a second strand, which can be connected together with a stem-loop adapter to form a single strand. A sequencing reaction can compare the two strands of the product, allowing the experimentalist to both map the sequence to a locus in a reference genome, as well as ascertain the methylation profile of the original target nucleic acid. | 10-29-2009 |
| 20090269772 | SYSTEMS AND METHODS FOR IDENTIFYING COMBINATIONS OF COMPOUNDS OF THERAPEUTIC INTEREST - Systems, methods, and apparatus for searching for a combination of compounds of therapeutic interest are provided. Cell-based assays are performed, each cell-based assay exposing a different sample of cells to a different compound in a plurality of compounds. From the cell-based assays, a subset of the tested compounds is selected. For each respective compound in the subset, a molecular abundance profile from cells exposed to the respective compound is measured. Targets of transcription factors and post-translational modulators of transcription factor activity are inferred from the molecular abundance profile data using information theoretic measures. This data is used to construct an interaction network. Variances in edges in the interaction network are used to determine the drug activity profile of compounds in the subset of compounds. The drug activity profiles are used to form a filter set of compound combinations from the subset of compounds. | 10-29-2009 |
| 20090269773 | METHODS OF DETERMINING THE HEALTH STATUS OF AN INDIVIDUAL - Methods of determining health status based on analysis of single cells in a sample or set of samples from an individual are described. | 10-29-2009 |
| 20090269774 | EVALUATION OF EOSINOPHILIC ESOPHAGITIS - A method to evaluate eosinophilic esophagitis based on information in an eosinophilic esophagitis transcriptome. | 10-29-2009 |
| 20090269775 | PROGNOSTIC MARKERS FOR CLASSIFYING COLORECTAL CARCINOMA ON THE BASIS OF EXPRESSION PROFILES OF BIOLOGICAL SAMPLES - The invention relates to the use of gene expression profiles for predicting the probability of recurrence or metastases to develop in remote organs of patients from which a primary colon carcinoma has been removed. | 10-29-2009 |
| 20090269776 | Magnetic Immunodiagnostic Method for the Demonstration of Antibody/Antigen Complexes especially of blood groups - The invention relates to a magnetic immunodiagnostic method for the demonstration of antibody-antigen complexes. One such method involves the research and/or identification of antibodies or antigens, preferably anti-antigen antibodies or antigens of a blood group, and comprises a suspension of magnetic particles coated with antigens that can be carried by cells such as erythrocytes. The invention also relates to a device and a kit for carrying out one such method. | 10-29-2009 |
| 20090269777 | IMMUNOASSAYS AND KITS FOR THE DETECTION OF NGAL - The present invention relates to NGAL immunoassays and kits, and to methods of using glycosylated mammalian NGAL and antibodies that bind to mammalian NGAL in immunoassays and kits. Among other things, the methods and kits can be employed to determine the amount of human NGAL monomer in a test sample, as well as to determine the proportion of human NGAL monomer to human NGAL dimer contained in a test sample. | 10-29-2009 |
| 20090269778 | BIOCOMPATIBLE THREE DIMENSIONAL MATRIX FOR THE IMMOBILIZATION OF BIOLOGICAL SUBSTANCES - The present invention relates to a method of producing a solid coated carrier carrying biological material. Furthermore, the invention relates to a solid coated carrier to which biological material is attached and uses of the solid coated carrier for the preparation of a medical product. Moreover, the invention provides a method for the contacting, filtration or cleaning of blood, lymph or liquor cerebrospinalis of a patient, a method for the diagnosis of a disease and a diagnostic composition. | 10-29-2009 |
| 20090269779 | Galectin-3 cleavage as a marker for matrix metalloproteinase activity in cancer - Provided are differential antibodies recognizing the cleaved and non-cleaved forms of matrix metalloproteinases (MMPs), and methods of using the antibodies as surrogate diagnostic markers for the presence of active MMPs in cancer, such as growing breast cancers. | 10-29-2009 |
| 20090269780 | Method for Creating a Standard for Multiple Analytes Found in a Starting Material of Biological Origin - The invention provides a method for creating a standard for multiple analytes comprising treating a portion of a sample to substantially remove analytes of interest to produce a series of specifically deficient samples; and determining and mixing an appropriate amount of the series of specifically deficient samples to create a standard. The analyte may be any substance to be measured. | 10-29-2009 |
| 20090269781 | Single-Molecule-Format Probe And Utilization Thereof - A single-chain probe of the present invention for detecting a ligand, comprises: a ligand binding protein for binding the ligand; a recognition protein for recognizing that the ligand is bound by the ligand binding protein; and C— and N-terminal fragments, generated by dissecting an enzyme, between the ligand binding protein and the recognition protein, wherein a carboxy terminal end of the C-terminal fragment is located upstream of an amino terminal end of the N-terminal fragment, and the C— and N-terminal fragments vary the enzyme activity via complementation in case where the recognition protein recognizes that the ligand is bound by the ligand binding protein. This makes it possible to achieve detection of a target protein-specific ligand using the single chain with a high efficiency. | 10-29-2009 |
| 20090269782 | DIAGNOSTIC METHOD FOR DETERMINING THE SUSCEPTIBILITY TO DELIVERY AND REAGENT KIT FOR USE THEREFOR - The invention relates to a diagnostic method for detecting susceptibility to delivery, and to a test kit for this purpose. A low, but higher than baseline level concentration of Insulin-like Growth Factor Binding Protein 1 (IGFBP-1), which is due to leakage from decidual cells, is detected by an immunological assay in a vaginal secretion sample. | 10-29-2009 |
| 20090269783 | B7-H2 molecules, novel members of the B7 family and uses thereof - Novel B7-like polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length B7-like proteins, the invention further provides isolated B7-like fusion proteins, antigenic peptides, and anti-B7-like antibodies. The invention also provides B7-like nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a B7-like gene has been introduced or disrupted. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided. | 10-29-2009 |
| 20090269784 | PROTEIN ENGINEERING OF MONOACYLGLYCEROL LIPASE (MGLL) - A number of soluble engineered forms of MGLL that are suitable for high-throughput screening and protein crystallization, as well as a crystallized form of monoacylglycerol lipase protein (MGLL) and descriptions of the X-ray diffraction patterns are disclosed. The engineered constructs of MGLL permit the expression and purification of protein suitable for crystallography or high-throughput screening and identification of ligands, which can function as active agents to MGLL. The X-ray diffraction patterns allow the three dimensional structure of MGLL to be determined at atomic resolution so that ligand binding sites on MGLL can be identified and the interactions of ligands with MGLL amino acid residues can be modeled. Models prepared using such maps permit the design of ligands which can function as active agents which include, but are not limited to, those that function as inhibitors of MGLL. | 10-29-2009 |
| 20090269785 | CRYSTAL STRUCTURE OF MONOACYLGLYCEROL LIPASE (MGLL) - A number of soluble engineered forms of MGLL that are suitable for high-throughput screening and protein crystallization, as well as a crystallized form of monoacylglycerol lipase protein (MGLL) and descriptions of the X-ray diffraction patterns are disclosed. The engineered constructs of MGLL permit the expression and purification of protein suitable for crystallography or high-throughput screening and identification of ligands, which can function as active agents to MGLL. The X-ray diffraction patterns allow the three dimensional structure of MGLL to be determined at atomic resolution so that ligand binding sites on MGLL can be identified and the interactions of ligands with MGLL amino acid residues can be modeled. Models prepared using such maps permit the design of ligands which can function as active agents which include, but are not limited to, those that function as inhibitors of MGLL. | 10-29-2009 |
| 20090269786 | RHO1-Gamma Amino Butyric Acid C Receptor-Specific Antibodies - This invention provides antibodies immunologically specific for ρ1-GABA | 10-29-2009 |
| 20090269787 | Monoclonal Antibody to CD166 and Method for Production Thereof - CD166 is a cell adhesion molecule belonging to an immunoglobulin superfamily that is expressed in an excessive amount on the tumor surface. If an monoclonal antibody specifically binding to the CD166 is obtained, it becomes possible to suppress growth of tumor cells, detect the cells, and supply a therapeutic drug thereto specifically. However, because the CD166 proteins are very similar to each other among mammals, it was not possible to obtain an antibody to human CD166, by immunizing, for example, mice with the human CD166. | 10-29-2009 |
| 20090269788 | METHOD FOR DETECTING MICROORGANISM - A method for detecting a microorganism in a sample containing or suspected of containing said microorganism, said method comprising: i) contacting said sample with a binding agent for said microorganism, wherein the binding agent is immobilised on a support, and allowing the binding agent to bind microorganism to form an immobilised complex; ii) separating the sample from the immobilised complex; iii) contacting the support with a liquid medium and a reagent which removes which eliminates, inactivates or inhibits a contaminant that may interfere with a microorganism detection assay; and iv) detecting microorganisms retained on the support using said microorganism detection assay. | 10-29-2009 |
| 20090269789 | ANTIBODIES FOR DETECTING MICROORGANISMS - An antibody for detection of microorganisms, a method of detection of microorganisms, and a reagent kit for detection of microorganisms, which is species specific for every species of microorganisms and with which all serototypes within the same species can be detected, are provided. Antibody to intracellular molecules with the same function in each type of microorganism, particular antibody to ribosomal protein, that is, ribosomal protein L7/L12, is made and antibody that reacts specifically with the microorganism in question is selected. | 10-29-2009 |
| 20090269790 | Method and Apparatus for Determining Hemocompatibility - Provided herein are techniques for screening materials for hemocompatibility. Hemocompatible materials may be advantageous when incorporated into devices that may come into direct contact with blood or other bodily fluids. Such techniques take advantage of conformational changes in fibrinogen when adsorbed onto certain materials. As a result of conformational changes, the fibrinogen has altered responsiveness to cleavage by thrombin. Accordingly, the products of thrombin cleavage of fibrinogen may be assessed to determine the hemocompatibility of a material. | 10-29-2009 |
| 20090269791 | NON-PROTEOLYTIC METHOD FOR THE DETERMINATION OF ANALYTES IN KERATINIZED STRUCTURES - Methods that permit the rapid release of one or more analytes from head or body hair or other keratinized structures of an individual (who may previously have ingested one or more of the analytes) are provided. The methods can include contacting the keratinized structure with a reducing agent but not with a proteolytic agent. The methods can further include identification and quantification of the one or more analytes by known analytical techniques such as immunoassays. The described methods do not damage the analyte and do not cause harmful effects on a subsequently-used analyte detection probe (e.g., an antibody). | 10-29-2009 |
| 20090269792 | ACETAMINOPHEN ASSAY - In general, the present invention provides a reliable assay for the quantitative determination of p-aminophenol in an aqueous sample. More particularly, the present invention provides a rapid enzyme-based assay for the quantitative determination of acetaminophen in a sample. The assay employs a xylenol chromophore and a catalyst that is preferably a weak oxidizer. The assay provides reliable results in the presence or absence of N-acetylcystiene (NAC) and can therefore be used to monitor acetaminophen levels during NAC treatment. Methods and kits for determining acetaminophen concentration in an aqueous sample are also provided. | 10-29-2009 |
| 20090269793 | Compounds and Kits for the Detection and the Quantification of Cell Apoptosis - The present invention relates a compound of formula (1) wherein: —R1 represents a linear alkyl group of 4 to 20 carbon atoms; —Y represents a linear alkyl group of 1 to 5 carbon atoms or a group of formula —R—O—R′—, —R—CO—R′— or —R—CO—NH—R′—, in which R represents a linear alkyl group of 1 to 3 carbon atoms, R′ represents a linear alkylene group of 0-3 carbon atoms, Y being linked to the bicycle in position 6 or 7; -Z represents a linear alkyl chain of 3 or 4 carbon atoms; -A represents an oxygen atom, a sulphur atom, or a —NH group, or an amminoalkyl group —NR″ in which R″ represents an alkyl group of 1 to 20 carbon atoms; —Ar represents an aromatic cycle or polycycle consisting of 6 to 14 carbon atoms, or an aromatic heterocycle, said heterocycle containing 4, 5 or 6 carbon atoms and at least one heteroatom selected in the group consisting of N, S, and O, or a condensed aromatic heterobicycle, said heterobicycle consisting of 6 to 9 carbon atoms and at least one heteroatom selected in the group consisting of N, S, and O; —R2 and R3, which are identical or different, each representing a hydrogen atom or an alkyl group of 1 to 4 carbon atoms, R2 and R3 optionally forming a 5- to 7-membered ring with the nitrogen atom; —R4, R5 and R6, identical or different, represent an hydrogen, a linear alkyl group or a linear oxyalkyl group of 1 to 4 carbon atoms. | 10-29-2009 |
| 20090269794 | SCREENING METHOD FOR COMPOUND INHIBITING DEVELOPMENT OR PROGRESSION OF FAMILIAL AMYOTROPHIC LATERAL SCLEROSIS AND DIAGNOSTIC METHOD FOR FAMILIAL AMYOTROPHIC LATERAL SCLEROSIS - A screening method comprising the following steps is provided.
| 10-29-2009 |
| 20090269795 | Mutant alpha4betadelta GABAA receptor and methods of treating anxiety or irritability - The present invention provides methods for treating anxiety or irritability in a subject. The methods comprise administering to the subject an effective amount of an antagonist of allopregnanolone (THP), or a regulator which decreases expression of the alpha 4 subunit of GABA such as gabadoxbol (THIP), or a vector comprising an isolated nucleic acid molecule encoding a mutant alpha 4 subunit GABA | 10-29-2009 |
| 20090269796 | METHODS OF DETECTING AND TREATING MYOCARDIAL ISCHEMIA AND MYOCARDIAL INFARCTION - Methods of detecting and treating myocardial ischemia and myocardial infarction based on the differential expression of metabolic products are described herein. | 10-29-2009 |
| 20090269797 | STRAINS OF ZYMOMONAS MOBILIS FOR FERMENTATION OF BIOMASS - The present invention relates to methods of obtaining | 10-29-2009 |
| 20090269798 | METHOD AND SYSTEM FOR SELECTING CHEMOTHERAPEUTIC AGENTS - Described herein is a method and corresponding system, including reagents and assays, for determining the in vivo efficacy of drugs in the course of chemotherapy. The method can include identifying a patient having a condition, such as leukemia, that is potentially susceptible to one or more candidate drugs having a particular in vivo biochemical activity, such as the generation of reactive oxygen or nitrogen species. An in vitro biochemical assay is provided that corresponds to the biochemical activity, and a tissue sample is tested to determine the responsiveness of the tissue sample to the biochemical activity, for instance, the ability of the tissue sample to degrade or inhibit the active species (in this case, hydrogen peroxide), and thereby prevent its desired effect against the cancerous cells. A course of chemotherapy can be based, at least in part, on the responsiveness of the tissue sample to the biochemical activity. | 10-29-2009 |
| 20090269799 | METHOD OF DETERMINING A COMPLETE BLOOD COUNT AND A WHITE BLOOD CELL DIFFERENTIAL COUNT - Systems and methods analyzing body fluids such as blood and bone marrow are disclosed. The systems and methods may utilize an improved technique for applying a monolayer of cells to a slide to generate a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and methods for utilizing multi color microscopy for improving the quality of images captured by a light receiving device. | 10-29-2009 |
| 20090269800 | DEVICE AND METHOD FOR PROCESSING CELL SAMPLES - A cell analysis device and method is described which enables the user to efficiently treat cells in a sample. The cells can be treated with reagents at a time and place proximate to collection and may be handled in a automated or semi automated fashion. | 10-29-2009 |
| 20090269801 | Vector to Induce Expression of Recombinant Proteins under Anoxic or Microaerobic Conditions - A protein expression vector that expresses large quantities of recombinant proteins under anoxic or microaerobic conditions by inducing expression with nitrate. The vector backbone is pUC19 and protein expression is driven by the | 10-29-2009 |
| 20090269802 | ALPHA 1,4-GALACTOSYLTRANSFERASE AND DNA ENCODING THEREOF - The object of the present invention is to provide α1,4-galactosyltransferase to transfer a galactose residue to C4 position of galactose residue of lactosylceramide or galactosylceramide, and DNA coding for the enzyme. | 10-29-2009 |
| 20090269803 | In Vivo Generation of Dna, Rna, Peptide, and Protein Libraries - The present invention relates to a method for the in vivo generation of DNA, RNA, peptide and protein libraries by means of a genetic element harboring a viral or phage origin of replication that is independently reproduced by a viral or phage error-prone polymerase not physically linked to the genetic element within a host cell furthermore containing viral or phage auxiliary nucleotide sequences and proteins that are required for replication of the viral or phage genetic element. The nucleotide or nucleotides of interest to be diversified are introduced into the genetic element and physically linked to the viral or phage origin of replication. | 10-29-2009 |
| 20090269804 | Methods for refolding proteins containing free cysteine residues - The present invention relates to novel methods for making and refolding insoluble or aggregated proteins having free cysteines in which a host cell expressing the protein is exposed to a cysteine blocking agent. The soluble, refolded proteins produced by the novel methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form PEGylated proteins. | 10-29-2009 |
| 20090269805 | Novel Lipolytic Enzyme ELIP - The present invention provides a novel nucleic acid sequence, designated ELIP, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same. | 10-29-2009 |
| 20090269806 | PROCESS FOR PRODUCING DIPEPTIDES - The present invention provides a process for producing a dipeptide which comprises culturing in a medium a microorganism which has the ability to produce a protein having the activity to form the dipeptide from one or more kinds of amino acids and which has the ability to produce at least one of said one or more kinds of amino acids, allowing the dipeptide to form and accumulate in the medium, and recovering the dipeptide from the medium. | 10-29-2009 |
| 20090269807 | Compositions and Methods for the Expression of Selenoproteins in Eukaryotic Cells - Recombinant nucleic acid constructs for the efficient expression of eukaryotic selenoproteins and related methods for production of recombinant selenoproteins are provided. The nucleic acid constructs comprise novel selenocysteine insertion sequence (SECIS) elements. Certain novel SECIS elements of the invention contain non-canonical quartet sequences. Other novel SECIS elements provided by the invention are chimeric SECIS elements comprising a canonical SECIS element that contains a non-canonical quartet sequence and chimeric SECIS elements comprising a non-canonical SECIS element that contains a canonical quartet sequence. The novel SECIS elements of the invention facilitate the insertion of selenocysteine residues into recombinant polypeptides. | 10-29-2009 |
| 20090269808 | VIRUS COAT PROTEIN VARIANTS WITH SELF-SUBTRACTING PROPERTIES - Herein is described a modified viral vector comprising: a coat protein modified, for example by the addition of a cysteine residue, such that the modified viral vector yields less soluble virus relative to that from an unmodified viral vector upon extraction of plant material infected with the modified viral vector, thereby facilitating purification of a recombinant protein expressed from the modified viral vector. Also described is a method of reducing viral coat protein impurities during purification of a recombinant protein, a method of biocontainment for a recombinant viral vector, and a method of generating virus inoculum for the modified viral vector. | 10-29-2009 |
| 20090269809 | THERMOSTABLE RIBONUCLEASE H - A polypeptide having an RNaseH activity and being highly useful in gene engineering; a gene encoding this polypeptide; and a genetic engineering process for producing the polypeptide. | 10-29-2009 |
| 20090269810 | Method for enhancing recombinant antibody production - The present invention is a method for enhancing recombinant antibody production by co-expressing in a host cell a recombinant antibody and ERp23 protein, which facilitates oxidative folding and stability of the recombinant antibody thereby enhancing production. | 10-29-2009 |
| 20090269811 | IL-1 RELATED POLYPEPTIDES - The present invention is directed to novel polypeptides having homology to the IL-1-like family of proteins and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention, and methods for producing the polypeptides of the present invention. | 10-29-2009 |
| 20090269812 | METHOD OF PRODUCING CHEMICAL PRODUCT AND CONTINUOUS FERMENTATION APPARATUS - The invention provides a method of producing a chemical product through continuous fermentation which includes filtering a culture of a microorganism or cultured cells with a separation membrane to recover a product from a filtrate and simultaneously retaining a nonfiltered fluid in, or refluxing it to, the culture, and adding fermentation materials to the culture, wherein a porous membrane having an average pore size of 0.01 μm or more to less than 1 μm is used as the separation membrane and the filtration is conducted with a transmembrane pressure difference in the range of 0.1 to 20 kPa. According to this method, the fermentation productivity of the chemical product can be largely elevated at high stability and a low cost. | 10-29-2009 |
| 20090269813 | Methods For Multiplexing Recombinase Polymerase Amphlification - This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes. | 10-29-2009 |
| 20090269814 | Method of Analyzing a BRCA2 Gene in a Human Subject - Five novel DNA and protein sequences have been determined for the BRCA2 gene, as have been ten polymorphic sites and their rates of occurrence in the normal alleles of BRCA2. The sequences BRCA2 | 10-29-2009 |
| 20090269815 | LINEAR AMPLIFICATION OF SHORT NUCLEIC ACIDS - The present teachings provide novel methods for amplifying short nucleic acids. In some embodiments, the present teachings provide novel methods for linearly amplifying a collection of micro RNAs by using temperature cycling during a reverse transcription reaction. The cycling can comprise at least 20 cycles of an annealing temperature segment of 10° C.-30° C., and a denaturation temperature segment of 35° C.-60° C. In some embodiments, the temperature cycled reaction can comprise an osmolyte. | 10-29-2009 |
| 20090269816 | System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts - The functional analysis of genes frequently requires the manipulation of large genomic regions. A yeast-bacteria shuttle vector is described that can be used to clone large regions of DNA by homologous recombination. Also described is a method for isolating entire genomes, including chloroplast genomes, or large portions thereof, and manipulating the same. Also described are methods for determining minimal genomes, minimal pathway requirements, and minimal organelle genomes. | 10-29-2009 |
| 20090269817 | PRETREATMENT OF GRAIN SLURRY WITH ALPHA-AMYLASE AND A HEMICELLULASE BLEND PRIOR TO LIQUEFACTION - A method of preparing a low viscosity slurry that includes grinding a small grain to produce a flour. The flour is mixed with water to form a slurry. An alpha-amylase enzyme and a hemicellulase blend enzyme are mixed into the slurry and allowed to convert the slurry into a mash. A saccharifying enzyme is mixed into the mash. It is possible to use coarse grains such as grain sorghum and maize in conjunction with the small grains. | 10-29-2009 |
| 20090269818 | Mashing Process - The present invention relates to a mashing and filtration step in a brewing process and to a composition useful in the mashing and filtration step of a brewing process. | 10-29-2009 |
| 20090269819 | METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF ANY OF THE cynT, cynS, cynX OR cynR GENE OR COMBINATION THEREOF - The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus | 10-29-2009 |
| 20090269820 | Dna coding for polypeptide participating in biosynthesis of pladienolide - The present invention provides polypeptides that participate in the biosynthesis of the pladienolide macrolide compounds, DNA that encodes these polypeptides and variants of this DNA, transformants that maintain all or a portion of this DNA or variant thereof, and a method of producing the pladienolide macrolide compounds using these transformants. More particularly, it provides an isolated pure DNA that contains at least one region encoding a polypeptide that participates in pladienolide biosynthesis; polypeptide encoded by this DNA; a self-replicating or integrated-replicating recombinant plasmid carrying this DNA; a transformant maintaining this DNA; and a method of producing a pladienolide, characterized by culturing this transformant on culture medium and collecting pladienolide from this culture medium. | 10-29-2009 |
| 20090269821 | Method for Preparing Optically Active 4-Hydroxy-1,2,3,4-Tetrahydroquinoline Compound - The present invention relates to a method for preparing an optically active 4-hydroxy-1,2,3,4-tetrahydroquinoline compound [I], which comprises the steps of: | 10-29-2009 |
| 20090269822 | MICROORGANISM - A microorganism which is | 10-29-2009 |
| 20090269823 | BUTANOL DEHYDROGENASE ENZYME FROM THE BACTERIUM ACHROMOBACTER XYLOSOXIDANS - From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of | 10-29-2009 |
| 20090269824 | Apparatus and method for fabricating Micro-Capsule - The present invention relates to an apparatus and a method for fabricating a microcapsule, more particularly to an apparatus and a method for fabricating a microcapsule, which enable to encapsulate uniform cell number in a microcapsule through cell distribution, improve cell viability in the microcapsule through fluid exchange, and ensure uniform microcapsule size. | 10-29-2009 |
| 20090269825 | METHOD FOR STABILIZATION OF BIOLOGICAL MOLECULE AND COMPOSITION - The object is to provide a method for stabilization of a biological molecule and a composition, specifically a method for stabilization of an enzyme or a labeled antibody for use in a clinical diagnosis and a composition. Thus, disclosed is a method for stabilization of a biological molecule which is characterized by allowing (a) the biological molecule and (b) sericin and/or a hydrolysate or equivalence thereof to coexist with each other. Also disclosed is a composition having a biological molecule stabilized therein, which is characterized in that the components (a) and (b) coexist with each other in the composition. Further disclosed is a composition for stabilizing a biological molecule, which comprises sericin and/or a hydrolysate or equivalence thereof. | 10-29-2009 |
