41st week of 2012 patent applcation highlights part 34 |
Patent application number | Title | Published |
20120258395 | TONER FOR DEVELOPING ELECTROSTATIC LATENT IMAGE - A toner for developing an electrostatic latent image includes toner particles. An average aspect ratio of the toner particles, having predetermined diameters of at least 3 μm and less than 10 μm, is in the range from about 0.820 to about 0.900, and the difference between the maximum value and minimum value among the average aspect ratios D3, D4, D5, D6, D7, D8, and D9 is up to 0.07. Dn represents an average aspect ratio of those toner particles having a diameter of at least n μm and less than n+1 μm. | 2012-10-11 |
20120258396 | Method for Preparing Toner Containing Carbon Black Pigment With Low Surface Sulfur Levels - Disclosed is a process for preparing toner particles which comprises: (a) selecting a carbon black; (b) measuring the surface level of sulfur of the carbon black by X-ray Photoelectron Spectroscopy to ensure that the surface level of sulfur is no more than about 0.05 atomic percent; and (c) mixing the carbon black with a resin to generate a toner composition. | 2012-10-11 |
20120258397 | CO-EMULSIFICATION OF INSOLUBLE COMPOUNDS WITH TONER RESINS - A process for making a latex emulsion suitable for use in a toner composition includes co-emulsifying a bio-based resin with an insoluble component, such as a pigment or wax, whereby the resin encapsulates the insoluble component. The resulting latex, including the insoluble component encapsulated in the resin, may then be utilized to form a toner. The insoluble component may thus be included in toner particles, which might otherwise be difficult to achieve, using emulsion aggregation processes. | 2012-10-11 |
20120258398 | TONER COMPOSITIONS AND PROCESSES - Toners are provided, which possess low melt properties capable of producing a low gloss finish. Methods for providing these resins and toners are also provided which include the process of controlling the gloss of the toner utilizing a slow acid addition during coalescence optionally combined with little or no chelating agent added during the aggregation process. | 2012-10-11 |
20120258399 | RADIATION-SENSITIVE RESIN COMPOSITION, COMPOUND AND PRODUCING METHOD OF COMPOUND - A radiation-sensitive resin composition includes a compound shown by a following general formula (A), a solvent and a resin having an acid-labile group. Each of R | 2012-10-11 |
20120258400 | NOVEL TARC MATERIAL FOR IMMERSION WATERMARK REDUCTION - A coating material for use during a lithography process. In an example, a coating material disposed on a material layer includes a polymer and a quencher catcher chemically bonded to the polymer. The quencher catcher substantially neutralizes any quencher that diffuses into the coating material from the material layer. | 2012-10-11 |
20120258401 | RESIST COMPOSITION AND METHOD FOR PRODUCING RESIST PATTERN - A resist composition contains (A) a resin being insoluble or poorly soluble in alkali aqueous solution, but becoming soluble in an alkali aqueous solution by the action of an acid, (B) an acid generator represented by the formula (II), and (D) a compound represented by the formula (I), | 2012-10-11 |
20120258402 | PHOTORESIST COMPOSITION, RESIST-PATTERN FORMING METHOD, POLYMER, AND COMPOUND - A photoresist composition includes a polymer that includes a structural unit shown by the following formula (1), and a photoacid generator. R | 2012-10-11 |
20120258403 | SALT AND PHOTORESIST COMPOSITION COMPRISING THE SAME - The present invention provides a salt represented by the formula (I): | 2012-10-11 |
20120258404 | RESIST COMPOSITION AND METHOD FOR PRODUCING RESIST PATTERN - A resist composition of the invention includes: (A) a resin being insoluble or poorly soluble in alkali aqueous solution, but becoming soluble in an alkali aqueous solution by the action of an acid, (B) an acid generator represented by the formula (II), and (D) a compound represented by the formula (I), | 2012-10-11 |
20120258405 | RESIST COMPOSITION AND METHOD FOR PRODUCING RESIST PATTERN - A resist composition includes; (A) a resin being insoluble or poorly soluble in alkali aqueous solution, but becoming soluble in an alkali aqueous solution by the action of an acid, (B) an acid generator having an acid-labile group; and (D) a compound represented by the formula (I), | 2012-10-11 |
20120258406 | APPARATUS AND METHOD FOR PREPARING RELIEF PRINTING FORM - The invention provides a method and an apparatus for preparing a relief printing form from a photosensitive element. More specifically, this invention describes a method and an apparatus for preparing a relief form in an environment having controlled oxygen concentration during exposure to actinic radiation. The method includes forming an in-situ mask on a photosensitive element, exposing the element to actinic radiation through the in-situ mask in an environment having an inert gas and a concentration of oxygen between 190,000 and 100 ppm, and treating the exposed element to form the relief printing form having a pattern of raised surface areas. | 2012-10-11 |
20120258407 | Multifield incoherent Lithography, Nomarski Lithography and multifield incoherent Imaging - A new optical method and apparatus, applicable to optical lithography, to imaging or to machine vision, including:
| 2012-10-11 |
20120258408 | ELECTROPLATING APPARATUS FOR TAILORED UNIFORMITY PROFILE - Methods of electroplating metal on a substrate while controlling azimuthal uniformity, include, in one aspect, providing the substrate to the electroplating apparatus configured for rotating the substrate during electroplating, and electroplating the metal on the substrate while rotating the substrate relative to a shield such that a selected portion of the substrate at a selected azimuthal position dwells in a shielded area for a different amount of time than a second portion of the substrate having the same average arc length and the same average radial position and residing at a different angular (azimuthal) position. For example, a semiconductor wafer substrate can be rotated during electroplating slower or faster, when the selected portion of the substrate passes through the shielded area. | 2012-10-11 |
20120258409 | DISTRIBUTED INJECTION WITH FUEL FLEXIBLE MICRO-MIXING INJECTORS - Provided is an injector having a plurality of micro-mixing nozzles having axes thereof pointing radially inwardly or outwardly with respect to a main axis of the injector and a plurality of micro-mixing nozzles having axes thereof extending axially with respect to the main axis of the injector. The arrangement of micro-mixing nozzles provides a means for fast and efficient mixing of fuels, such as highly reactive fuels including hydrogen. The arrangement of micro-mixing nozzles also achieves low NOx emissions. | 2012-10-11 |
20120258410 | OXY-HYDROGEN FUEL INTEGRATION AND CONTROL SYSTEM - An oxy-hydrogen generator control system may include an oxy-hydrogen generator for fuel production on demand. A controller may determine if ignition is present in a burner assembly prior to generating the oxy-hydrogen fuel and providing it to the burner assembly. The controller may also purge system lines of any residual oxy-hydrogen fuel when heating is no longer needed. | 2012-10-11 |
20120258411 | SNOW MELTING APPARATUS - A snow melting apparatus in which a heating assembly is attached to a motion enabling apparatus such as the chassis and engine of a fork lift truck or a ride lawn-mower, or the chassis of a push lawn-mower. The heating assembly is an array of fuel burning nozzles oriented vertically downward, and vertically adjustable in height with respect to the snow surface. The fuel may be propane or fuel oil, stored in a pressure resistant cylinder. The snow melting apparatus may include an air blowing apparatus, such as an electric fan, so as to provide increased oxygen and to direct heat down from the nozzles toward the surface the heater is melting. A de-icing mixture dispenser may also be attached to the unit. The snow melter may be made in a ride version, a push version and a detachable version designed to fit onto commonly available devices such as lawn-mowers. | 2012-10-11 |
20120258412 | AUTOMATED TORCH - An automated torch for generating and sustaining a flame is provided that includes a head, the head including a burning chamber, a pole, a computer module, at least one fuel supply tube, a valve, a diffuser assembly and an igniter/sensor. | 2012-10-11 |
20120258413 | METHOD OF ADJUSTING INSULATION IN A DIRECTIONAL SOLIDIFICATION FURNACE - Methods are disclosed for inhibiting heat transfer through lateral sidewalls of a support member positioned beneath a crucible in a directional solidification furnace. The methods include the use of insulation positioned adjacent the lateral sidewalls of the support member. The insulation inhibits heat transfer through the lateral sidewalls of the support member to ensure the one-dimensional transfer of heat from the melt through the support member. | 2012-10-11 |
20120258414 | SUBSTRATE SUPPORT INSTRUMENT, AND VERTICAL HEAT TREATMENT APPARATUS AND DRIVING METHOD THEREOF - A substrate support instrument includes a first support instrument portion and a second support instrument portion detachably combined with each other. Each of the first support instrument portion and second support instrument portion includes: a ceiling plate and a bottom plate facing each other upward and downward; a support pillar disposed in plurality along a peripheral edge portion of each of the ceiling plate and bottom plate, and configured to connect the ceiling plate and the bottom plate; and a support part disposed at a position corresponding to each of the support pillars, and configured to support a bottom of each substrate. In the support part, a height position is set such that a substrate supported in the first support instrument portion and a substrate supported in the second support instrument portion are alternately arranged, when the first support instrument portion is combined with the second support instrument portion. | 2012-10-11 |
20120258415 | HEAT TREATMENT APPARATUS AND HEAT TREATMENT METHOD - A heat treatment apparatus including: a processing container for processing wafers held in a boat; heaters for heating the processing container; and a control section for controlling the heaters. Heater temperature sensors are provided between the heaters and the processing container, in-container temperature sensors are provided in the processing container, and movable temperature sensors are provided in the boat. The temperature sensors are connected to a temperature estimation section. The temperature estimation section selects two of the three types of temperature sensors, e.g. the movable temperature sensors and the in-container temperature sensors, and determines the temperature of a wafer according to the following formula: T=T | 2012-10-11 |
20120258416 | METHOD TO DEFINE, MEASURE, AND DISPLAY MESIODISTAL ANGULATION AND FACIOLINGUAL INCLINATION OF EACH WHOLE TOOTH - This is directed to systems, processes, machines, and other means that determine the mesiodistal angulation and the faciolingual inclination of each whole tooth in three-dimensional space in the field of orthodontics and displaying the results in constructed panoramic views. | 2012-10-11 |
20120258417 | ELASTIC BANDS FOR ORTHODONTIC USE - An orthodontic band is provided made from an elastomeric material wherein the band has a shape which simulates the profile of an object with an open area within the band. In its unstretched condition the outer perimeter of the band is of a shape which is not a smooth continuous endless curve to thereby simulate the profile of the object, such as a star or heart. The band is mounted to a bracket on an upper tooth and to a bracket on a lower tooth by stretching the band and looping the open area over hooks on both brackets. The stretched band thereby provides a force against the teeth. | 2012-10-11 |
20120258418 | Portable Simultaneous Tooth Prophylaxis And Bleaching System - A portable dental prophylaxis and bleaching system is provided for simultaneous tooth polishing and bleaching. It includes an oscillatory driving handpiece, a dental angle with a disposable rubber cup assembly, a dual-component dispenser, and a disposable mouthpiece. The battery-powered handpiece transforms a continuous rotation through a driving cam into a rotary oscillation which is then transferred through the attached dental angle to the rubber cup with an obtuse angle at its distal output end. The dispenser is operated manually to correctly apportion and mix the dual treatment materials into the rubber cup. In order to provide longer shelf life and effective bleaching action, the treatment materials are maintained at optimal pH values in separate compartments within the dispenser before use. The disposable mouthpiece is shaped for easy access to the user's teeth while protecting the gum surfaces. The system can be used both in dental clinics and at home. | 2012-10-11 |
20120258419 | Medicine Delivery System - An injection assembly for injecting medicine. The assembly includes a selectively collapsible elastomeric bulb having a hollow interior and an outlet. The bulb is collapsible from an expanded configuration in which the hollow interior has an expanded volume to a collapsed configuration in which the hollow interior has a collapsed volume that is smaller than the expanded volume. The assembly includes a tube having a proximal end connected to bulb, a distal end, and an interior passage in fluid communication with the outlet of the bulb. The assembly includes a needle connected to the distal end of the tube having a sharp distal end. The needle has an interior passage in fluid communication with the interior passage of the tube for delivering medicine from the hollow interior of the bulb when the bulb is compressed and drawing fluid through the needle toward the bulb when the bulb expands. | 2012-10-11 |
20120258420 | DISPENSING DEVICE FOR A DENTAL MATERIAL AND A METHOD OF FILLING THE DEVICE - A dispensing device for a dental material, comprises a container that has a passageway with a front opening for releasing the dental material, and a rear opening. The device has a first fill opening in the passageway arranged between the rear opening and the front opening. The device further has a second fill opening. The device is adapted to receive a piston in the passageway which is movable between the rear opening and the front opening. | 2012-10-11 |
20120258421 | Anesthetic Syringe - A syringe assembly for dispensing medicine from a cartridge having a diaphragm and a piston opposite the diaphragm. The assembly includes a barrel having a hollow interior, an open proximal end, a closed distal end, and an outlet. The assembly also includes a cartridge receiver slidably receivable in the hollow interior of the barrel. The receiver includes an interior space for receiving the cartridge, an access needle extending into the interior space for puncturing the diaphragm of the cartridge. The access needle directs fluid to the barrel outlet. The cartridge receiver includes a plunger rod movable into the interior space of the cartridge receiver for engaging the piston of the cartridge to selectively force medicine in the cartridge through the access needle. | 2012-10-11 |
20120258422 | MIXING AND APPLICATION CAPSULE FOR PRODUCING A DENTAL PREPARATION - A mixing and application capsule for producing a dental preparation is described. The mixing and application capsule can have a capsule body with a mixing space for receiving a mixture component and for mixing the dental preparation from the mixture component and a fluid and with an outlet orifice for expelling the dental preparation, a cavity for receiving the fluid, a piston body that is movable in the capsule body, and delimits the mixing space in the capsule body and has a channel for conveying the fluid from the cavity into the mixing space. The piston body includes wholly or partly of two or a plurality of dissimilar materials, comprising at least one soft component and at least one hard component | 2012-10-11 |
20120258423 | CAPSULE AND DELIVERY TIP WITH TRANSITION PORTION FOR DISPENSING VISCOUS REACTIVE DENTAL MATERIALS - A material capsule containing a reactive or caustic dental material and a separately attachable delivery tip having a metal cannula. A material capsule for placement in a syringe contains a reactive or caustic dental material for dispensing through a metal cannula. The material capsule is sealed for storing the caustic or reactive dental material, preventing prolonged contact with or proximity to the metal cannula of a delivery tip. Contact with the reactive or caustic dental material only occurs during actual dispensing. Transition portions facilitate dispensing of viscous material. External grips facilitate attachment of the delivery tip. The dispensing and accurate placement of caustic or reactive dental materials is facilitated, making dentistry easier. | 2012-10-11 |
20120258424 | DENTAL AUTO-MIXING METHOD, DEVICE, AND COMPOSITION FOR TEMPORARY CEMENTS - The abstract has been amended as follows: A method of dispensing a hardenable dental composition, which can form a temporary bond, comprising 1) providing multi-part hardenable dental composition comprising part (A) in the form of a paste comprising acid-reactive glass particles and a liquid selected from the group consisting of water, a monomer having at least one ethylenically 10 unsaturated group per monomer molecule, and a combination thereof; and part (B) comprising a water soluble polyacid and a liquid selected from the group consisting of water, a monomer having at least one ethylenically unsaturated group per monomer molecule, and a combination thereof; and 2) extruding the composition through a static mixer in fluid communication with a first reservoir containing the part (A) and a second reservoir 20 containing the part (B); wherein a plunger is positioned in each reservoir for simultaneously forcing part (A) and part (B) into the static mixer. | 2012-10-11 |
20120258425 | APPARATUS FOR REMOVING ENAMEL OR DEBRIS FROM A TOOTH - An apparatus for removing enamel or debris from a tooth includes a blade having an abrasive surface and a handle portion. The handle portion includes a support portion for mounting the blade and a shank having a proximal end connected to the support portion and a distal end. The shank includes a flexible shank portion having two flexible elements separated by a slit extending axially from the distal end of the shank along the flexible shank portion. | 2012-10-11 |
20120258426 | Dental prosthetic device with remoldable base - A dental prosthetic device ( | 2012-10-11 |
20120258427 | DENTAL ABUTMENT INCLUDING FILLET - Exemplary embodiments relate to a dental abutment and methods of forming the same. The abutment may include a head portion located at an upper end of the abutment and constructed to support a prosthetic tooth replacement and soft tissue adjacent the head portion, an anti-rotation feature located at a lower end of the abutment and constructed to mate with a dental implant, and a fillet located at an interface between the head portion and the anti-rotation feature. The fillet may reduce a risk of fracture at the interface between the head portion and the anti-rotation feature. In some examples, the abutment may be formed at least partially of a ceramic material. | 2012-10-11 |
20120258428 | MODULATING LASER FOCAL LENGTH TO OPTIMIZE SURFACE TEXTURING ON MULTIPLE SURFACES - A system and method for applying a uniform micro-textured surface treatment to a deep-thread dental implant by rapidly modulating the focal point of a laser to laser etch surfaces of both the thread peaks and the thread valleys in a single pass. | 2012-10-11 |
20120258429 | DENTAL IMPLANT SYSTEM WITH A BONE SUPERSTRUCTURE AND METHOD FOR MANUFACTURING SUCH A BONE SUPERSTRUCTURE - The invention concerns a method for manufacturing a bone superstructure ( | 2012-10-11 |
20120258430 | PRODUCTION OF INDIVIDUAL DENTAL PROSTHESIS VIA CAD/CAM AND RAPID MANUFACTORING/RAPID PROTOTYPING FROM DATA OF THE DIGITAL IMPRESSION - A method for automated manufacture of dentures including providing a digital data set of the individual denture to be produced, a digital separation of the model into dental arch and gingival, producing the dental arch from a ceramic or plastic material using cutting technology, producing the denture base through generative or ablative procedures from predominantly (meth-)acrylate-based plastic materials, connecting the dental arch and gingiva through bonding or joining or a combination of bonding and joining. | 2012-10-11 |
20120258431 | Method and System for Tracking Jaw Motion - Methods are provided to record and utilize jaw motion data using low-angle photogrammetric techniques. A method is described based on using an extraoral imaging apparatus to capture images of texture placed on tooth or soft tissue surfaces. Upper and lower textural surface regions of the oral cavity are imaged simultaneously, and their surfaces derived as a function of time to produce 4d data. The clinically derived surface data may be directly registered to the 3d anatomy of an individual, providing the ability to animate the relative motion of the mandible. An imaging apparatus suitable for capture of low-angle image groups is disclosed. | 2012-10-11 |
20120258432 | Target Shooting System - The target shooting system provides a target shooter with immediate feedback, both visual and auditory, on the shooter's performance. The system comprises a real-time imaging feed of a target being shot at by a target shooter; wherein the image is submitted to a computing device running a software program. The program processes the images in such a manner as to be able to present the shooter with both audio and visual feedback of where the shooter's shots are hitting the target. The audio feedback comprises real-time announcement of a hit location relative to a pre-set bullseye (for example: “Hit one inch high, point five inch right.”). The visual feedback comprises a real-time image of the target with any hits on the target highlighted by an indicator, such as an outline of concentric, colored circles, or any other suitable indicator. | 2012-10-11 |
20120258433 | Fitness Monitoring Methods, Systems, And Program Products, And Applications Thereof - Program products, methods, and systems for providing fitness monitoring are disclosed. In an embodiment, a method for scheduling training activities for a user of a fitness monitoring device includes: defining a cardio training plan including one or more cardio training activities and defining a non-cardio training plan including one or more non-cardio training activities; scheduling execution of the non-cardio training plan and the cardio training plan in a training calendar; and providing a graphical display of the training calendar including the cardio training plan and the non-cardio training plan to the user of the fitness monitoring device. | 2012-10-11 |
20120258434 | CONTROL OF SOFTWARE APPLICATION FOR LEARNER RESPONSE SYSTEM - There is disclosed a method, in a learner-response system comprising a computer system and a plurality of user terminals adapted to communicate with the computer system, the method comprising: providing a presentation application adapted for displaying a set of questions associated with a learner-response session via a user interface of the presentation application; providing a learner-response application adapted to be responsive to inputs received from the plurality of user terminals; wherein the method further comprises: forwarding inputs detected by the presentation application as being associated with a learner-response session to the learner-response application; processing such inputs at the learner-response application. | 2012-10-11 |
20120258435 | METHOD FOR CONDUCTING AN ASSESSMENT AND A PARTICIPANT RESPONSE SYSTEM EMPLOYING THE SAME - A computerized method comprises creating an answer key for an assessment comprising one or more questions to be delivered to one or more participants, where the answer key comprises assessment information and question information; delivering the assessment to the participants; collecting responses from the participants; and saving question descriptions, any annotations made thereon and the collected responses. | 2012-10-11 |
20120258436 | AUTOMATED ASSESSMENT OF COGNITIVE, FINE-MOTOR, AND MEMORY SKILLS - An automated assessment of various capabilities, such as cognitive, fine-motor, and memory skills, includes the use of Tangible Geometric Games (TAG-Games), which are a play-based assessment tools. TAG-Games are based on Sensor-Integrated Geometric Blocks (SIG-Blocks) and an interactive graphical user interface (GUI), which provide a means for real-time and remote monitoring of a user through operative communication between blocks and a remote computer. The data made available by employing TAG-Games includes: 1) block accelerations, 2) time at stages of assembly completion, 3) total completion time for quizzes, and 4) correctness of assembly steps. In addition, a GUI can display the real-time assembly configuration of the blocks. | 2012-10-11 |
20120258437 | CONTEXT-AWARE TRAINING SYSTEMS, APPARATUSES, AND METHODS - Context-aware training systems, apparatuses and systems. The context-aware training systems, apparatuses and systems are computer-implemented and include sensing a user action and, based on a training needs model, estimating a cost or benefit to exposing the user to a training action, selecting a training action from a collection of available training actions and delivering the training action to the user if the user action indicates a need for the user to be trained and the cost or benefit to exposing the user to the training action indicates user exposure to the training action is warranted. | 2012-10-11 |
20120258438 | SYSTEMS AND METHODS FOR COMPUTERIZED INTERACTIVE TRAINING - The present invention is related to interactive training. In one embodiment, a training system presents a stored scenario to a user via a terminal. The user is provided with the ability to intervene and stop the pre-recorded scenario to identify an error in handling the situation presented in the scenario and/or an opportunity presented in the scenario. Once the user has intervened, the user can be presented with a question regarding the opportunity and/or error. The user is scored based in part on the number of errors and/or opportunities that the user identified and optionally on the user's response to the question. | 2012-10-11 |
20120258439 | Protein-Free Culture Media Products - Disclosed herein are compositions and methods for a substantially protein-free media (PFM) optimized for stem cell research. | 2012-10-11 |
20120258440 | METHOD OF DECIDING DETACHMENT TIME FOR CELL, METHOD OF SUBCULTURING CELL AND APPARATUS FOR SUBCULTURING CELL USING THE SAME - A method of deciding detachment time of cells, and a method and apparatus for subculturing the cells using the method. The method of deciding detachment time of the cells from a culturing vessel using a separation enzyme in subculturing the cells includes: a photographing step of photographing an image of the cells treated with the enzyme; a computing step of calculating a contrast value of the photographed image, and computing a change rate of the contrast value; a comparison step of repeating the photographing step and the computing step until an increasing change rate of the contrast value is reduced to a predetermined change rate or less; and a reporting step of reporting a time to remove the enzyme from the cells and to detach the cells from the culturing vessel when the increasing change rate of the contrast value is reduced to the predetermined change rate or less. | 2012-10-11 |
20120258441 | METHOD FOR CONTROLLING CULTURE PARAMETERS IN A BIOREACTOR - A method for controlling at least one culture parameter in a bioreactor bag ( | 2012-10-11 |
20120258442 | DETERMINING TUMOR ORIGIN - The disclosure provides methods for the use of gene expression measurements to classify or identify among 54 cancer types in samples obtained from a subject in a clinical setting, such as in cases of formalin fixed, paraffin embedded (FFPE) samples. | 2012-10-11 |
20120258443 | METHODS FOR THE DETECTION OF JC POLYOMA VIRUS - Methods and compositions for determining whether a subject is at risk for PML, including subjects being treated with immunosuppressants, by determining whether the subject harbors a JCV variant with reduced binding for sialic acid relative to a normal JCV, are presented. Furthermore, combinations of JCV-VP1 sequence variations that are associated with PML and that can be used as a basis of an assay for identifying subjects susceptible to PML, subjects with PML (e.g., early stage PML), or subjects at risk of developing PML in response to an immunosuppressive treatment are provided. | 2012-10-11 |
20120258444 | ACOUSTIC WAVE (AW) SENSING DEVICES USING LIVE CELLS - In one embodiment according to the invention, there is provided a method of sensing a response of a living cell or virus to a change in conditions. The method comprises applying an essentially constant external electromotive force that causes oscillation of an acoustic wave device at essentially constant amplitude and frequency under steady state conditions. The acoustic wave device has attached at least one living cell or virus. A combined oscillating system including the acoustic wave device and the living cell or virus exhibits a fundamental frequency and at least one harmonic frequency of the combined oscillating system. The living cell or virus is exposed to a change in an environmental condition while oscillating the combined oscillating system under the essentially constant external electromotive force, whereby a response of the living cell or virus to the change in environmental condition will be indicated by a change in at least one of frequency and amplitude of the oscillation of at least one harmonic frequency of the combined oscillating system. | 2012-10-11 |
20120258445 | METHODS FOR USING NANOWIRE SENSORS - Methods for using nanowire sensors are described. In one embodiment, the nanowire sensor may be field effect transistor having a nanowire and a functionalized control electrode. One method of using such a nanowire sensor includes exposing the functionalized control electrode to a test sample and an enhancing reagent. In another embodiment, the nanowire sensor may be a field effect transistor having a gate electrode and a functionalized nanowire. One method of using such a nanowire sensor includes exposing the functionalized nanowire to a test sample and an enhancing reagent. The use of an enhancing reagent increases the sensitivity of the nanowire sensor to a substance to be detected or quantified. | 2012-10-11 |
20120258446 | Universal Multi-Variant Detection System - The present invention provides a method to diagnostically detect the variants of a given pathogen, such as HIV, hepatitis C, hepatitis B (HBV), Parvovirus B19, etc., with the use of a single detection probe. | 2012-10-11 |
20120258447 | REAL-TIME MULTIPLEXING DETECTION OF TARGET NUCLEIC ACID SEQUENCES WITH ELIMINATION OF FALSE SIGNALS - The present invention relates to the real-time multiplex detection of at least three target nucleic acid sequences with elimination of false positive signals. Unlikely to conventional real-time multiplex PCR methods, the present invention comprises two different amplification reactions in different reaction vessels from each other: a primary multiplex PCR for obtaining amplicons and a secondary nested real-time multiplex PCR using the amplicons. The present invention permits to eliminate the false positive signals generated by the dimer formation of labeled primers, false positive signals | 2012-10-11 |
20120258448 | MUTATIONAL PROFILE IN HIV-1 GAG CLEAVAGE SITE CORRELATED WITH PHENOTYPIC DRUG RESISTANCE - The invention concerns novel mutations or mutational profiles of HIV-1 protease cleavage sites (CS) in the Gag region correlated with a phenotype causing alterations in sensitivity to anti-HIV drugs. The present invention also relates to the use of genotypic characterization of a target population of HIV and the subsequent association, i.e., correlation, of this information to phenotypic interpretation in order to correlate virus mutational profiles with drug resistance. The invention further relates to methods of utilizing the mutational profiles of the invention in databases, drug development, i.e., drug design, and drug modification, therapy and treatment design and clinical management. | 2012-10-11 |
20120258449 | METHOD OF DETERMINING THE NUCLEOTIDE SEQUENCE OF OLIGONUCLEOTIDES AND DNA MOLECULES - The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions. | 2012-10-11 |
20120258450 | Method for Monitoring Gene Expression of Translation and Integral/Secretory Protein Synthesis by Magnetic Resonance Spectroscopy (MRS) - Disclosed is a method for monitoring change in gene expression of translation and integral/secretory protein synthesis in a sample comprising measuring the amount of Choline and/or a fatty acid present in the sample as a function of time wherein the measurement is carried out by Magnetic Resonance Spectroscopy (MRS) and correlating the amount of Choline and/or fatty acid measured as function of time to the change in gene expression of translation and integral/secretory protein synthesis in the sample. The level of Choline from organelles matches the level of Choline from transcription and integral/secretory protein synthesis. Quantification of translation and integral/secretory protein synthesis leads to quantification of genetic expression. During apoptosis the amount of Choline decreases, and the amount of fatty acids increase from distinct trafficking of Choline polar heads and fatty and tails of degraded endomembranes. | 2012-10-11 |
20120258451 | LASER ISOLATION OF VIABLE CELLS - Methods for laser microdissection isolation of viable cells are provided. Cells of a desired type may be isolated from a diverse population, optionally with detection and exclusion of undesired cells. Desired cells may be isolated from a population that arose from differentiation of pluripotent cells, preferably embryonic stem cells or induced pluripotent stem cells, and undifferentiated stem cells may be detected and excluded from selection including the isolation of RPE cells sleeted based on morphology (e.g., characteristic mottled appearance) from a population of ES cells. The cells isolated by these methods, including RPE cells, may be essentially free of undifferentiated cells and thus suitable for use in cell-based therapies. | 2012-10-11 |
20120258452 | DNA-BASED MOLECULAR SWITCHES AND USES THEREOF - Disclosed are nucleic acid-based molecular switches that respond to changes in pH. The switches may be used in DNA nanodevices. The switches may also act as sensors for measuring the pH of a sample, including cells, regions thereof, and whole organisms. The switch includes an A-motif that forms at acidic pH. Also disclosed are compositions and methods for measuring the pH of cells or regions thereof, such as vesicles, the nucleus, mitochondrial matrix, or the Golgi lumen. | 2012-10-11 |
20120258453 | NUCLEIC ACID PROBE-BASED DIAGNOSTIC ASSAYS TARGETING SSRA GENES OF PROKARYOTIC AND EUKARYOTIC ORGANISMS - Use of the ssrA gene or tmRNA, an RNA transcript of the ssrA gene, or fragments thereof as target regions in a nucleic acid probe assay for the detection and identification of prokaryotic and/or eukaryotic organisms is described. Nucleotide sequence alignment of tmRNA sequences from various organisms can be used to identify regions of homology and non-homology within the sequences which in turn can be used to design both genus specific and species specific oligonucleotide probes. These newly identified regions of homology and non-homology provide the basis of identifying and detecting organisms at the molecular level. Oligonucleotide probes identified in this way can be used to detect tmRNA in samples thereby giving an indication of the viability of non-viral organisms present in various sample types. | 2012-10-11 |
20120258454 | Bisulfite conversion of DNA - The present invention relates to an improved method for the bisulfite conversion of DNA. In certain time-temperature ranges the efficacy of the bisulfite conversion is clearly improved. By combination with denaturating solvents, new reaction conditions and new purification methods the efficacy can be further increased The converted DNA can subsequently be analysed by different methods. The present invention facilitates the analysis of cytosine methylation. | 2012-10-11 |
20120258455 | RNase H-Based Assays Utilizing Modified RNA Monomers - The present invention provides methods of cleaving a nucleic acid strand to initiate, assist, monitor or perform biological assays. | 2012-10-11 |
20120258456 | MONITORING RECOMBINASE POLYMERASE AMPLIFICATION MIXTURES - A process includes providing a mixture that includes a recombinase, a single-strand binding protein, and one or more oligonucleotides; and detecting particles in the reaction mixture. | 2012-10-11 |
20120258457 | Methods for Detection of a Single- or Double-Stranded Nucleic Acid Molecule - The present invention is related to a method for the detection of a nucleic acid molecule comprising at least a strand comprising a sequence of nucleotides in a sample, whereby the method comprises the following steps: providing a sample containing the nucleic acid molecule; providing a capture probe, whereby the capture probe is at least partially complementary to a part of the nucleic acid molecule; allowing the capture probe to react with the nucleic acid molecule or a part thereof; and detecting whether or not the capture probe is hybridized to the nucleic acid molecule or part thereof. | 2012-10-11 |
20120258458 | INTERFERON-LIKE PROTEIN ZCYTO21 - The present invention relates to polynucleotide and polypeptide molecules for Zcyto21, an interferon-like protein, which is most closely related to interferon-α at the amino acid sequence level. The present invention also includes antibodies to the Zcyto21 polypeptides, and methods of using the polynucleotides and polypeptides. | 2012-10-11 |
20120258459 | SYSTEM AND METHOD FOR PARTICLE FILTRATION - Embodiments of the present disclosure feature a filtration system comprising a filtration module for particle filtration and methods of using the device for the isolation of particles (e.g., viable cells). Advantageously, embodiments of the device provide for the high throughput filtration of large volumes of sample while preserving cell viability and. providing high yields. | 2012-10-11 |
20120258460 | Sso7-Polymerase Conjugates with Decreased Non-Specific Activity - Improved Sso7-polymerase conjugate proteins are provided | 2012-10-11 |
20120258461 | METHODS FOR DETERMINING AND INHIBITING RHEUMATOID ARTHRITIS ASSOCIATED WITH THE BRAF ONCOGENE IN A SUBJECT - The invention provides methods for determining whether a subject is suffering from a rheumatoid arthritis associated with the BRAF oncogene comprising contacting isolated fibroblasts from the subject with a molecule or pool of molecules directed to the BRAF oncogene; and culturing the sample in the presence of the agent and determining whether BRAF oncogene expression by the cell is decreased and/or whether cells in the sample return to a less transformed phenotype, exhibit decreased cell proliferation and/or exhibit increased contact inhibition, any of which is indicative that the subject is suffering from a rheumatoid arthritis associated with the BRAF oncogene. | 2012-10-11 |
20120258462 | COMBINATIONS OF TUMOR-ASSOCIATED ANTIGENS IN DIAGNOSTICS FOR VARIOUS TYPES OF CANCERS - Disclosed herein are methods for matching a cancer condition with an appropriate immunotherapeutic agent and/or regimen. Also disclosed are methods for confirming diagnosis of a particular type of cancer. Embodiments of the invention disclosed herein are directed to the use of effective combinations of TuAAs to optimize the match between a patient's cancer condition and available immunotherapies. | 2012-10-11 |
20120258463 | RACK FOR SAMPLE TUBES AND REAGENT HOLDERS - A rack for holding samples and various reagents, wherein the rack may be used for loading the samples and reagents prior to using the reagents. The rack accepts complementary reagent holders, each of which contain a set of reagents for carrying out a predetermined processing operation, such as preparing biological samples for amplifying and detecting polynucleotides extracted from the samples. | 2012-10-11 |
20120258464 | METHOD FOR DETECTION AND QUANTIFICATION OF WHEAT ENDOGENOUS GENE - Provided is a method of detecting or quantifying a wheat species-specific DNA in a test sample by polymerase chain reaction. The method comprises a step of amplifying a nucleic acid molecule having a partial sequence of a nucleotide sequence identified as SEQ ID NO: 1 using a nucleic acid molecule in the test sample or a nucleic acid molecule extracted from the test sample as the template and using a primer pair capable of amplifying the partial sequence and a step of detecting or quantifying the amplified nucleic acid molecule. | 2012-10-11 |
20120258465 | MUTANT APOPROTEIN OF PHOTOPROTEIN WITH LOW CALCIUM SENSITIVITY - Calcium-binding photoproteins showing the luminescence pattern with a slow decay of are desired. The invention provides a mutant apoprotein comprising an amino acid sequence wherein the 23rd to 34th amino acids in the amino acid sequence of SEQ ID NO: 2 are substituted with an amino acid represented by formula I below: Xaa23-Xaa24-Xaa25-Xaa26-Xaa27-Xaa28-Xaa29-Xaa30-Xaa31-Xaa32-Xaa33-Xaa 34; having a function to bind to the peroxide of coelenterazine or the peroxide of a coelenterazine analogue to form a photoprotein capable of emitting light under the action of calcium ions; and, having a half decay time of the luminescence emitted by binding of the photoprotein to calcium ions being not less than 2 seconds. | 2012-10-11 |
20120258466 | FILTRATION DEVICE FOR ASSAYS - A device and method for filtering blood is disclosed herein. The device can filter blood and attach analytes within the blood to magnetic particles. The analytes can then be strongly bound to an analyzing device by a magnetic force. The analytes can then be counted by the analyzing device and the result can be displayed. | 2012-10-11 |
20120258467 | TRANSDERMAL SYSTEMS, DEVICES, AND METHODS TO OPTICALLY ANALYZE AN ANALYTE - The invention provides transdermal optical analysis systems, test sensors, methods, and kits for determining the presence and/or concentration of at least one analyte in a fluid sample. The system includes a transdermal test sensor including an aqueous material including at least one analyte selective reagent and at least one optically active moiety. The optical system preferably uses fluorescent spectroscopy to correlate fluorescent emission or adsorption from a dye with the analyte concentration of the sample. An optical light source and/or detector may be housed with the aqueous material in a housing or external to the housing of the aqueous material. | 2012-10-11 |
20120258468 | INDICATOR AGENT FOR NONINFLAMMATORY STRESS RESPONSE AND USE THEREOF - A system enabling the molecular biological visualization and quantitative detection of events in a stress-exposed living organism and a means enabling the management of stress are provided. An indicator agent for non-inflammatory stress responses mediated by superoxide, which comprises IL-18, a visualizing agent for non-inflammatory stress responses for detecting the aforementioned indicator agent, a method of measuring the degree of non-inflammatory stress, which comprises using the aforementioned visualizing agent, a method of preventing, ameliorating or predicting a change in immune status based on a non-inflammatory stress response, which comprises applying the aforementioned visualizing agent to an animal, and a therapeutic agent for a change in immune status based on a non-inflammatory stress response for reducing the amount or activity of the aforementioned indicator agent. | 2012-10-11 |
20120258469 | Methods And Devices For Using Mucolytic Agents Including N-Acetyl Cysteine (NAC) - Devices and methods incorporate mucolytic agents into a point-of-care testing device. The sample is loaded, and then the sample travels until it encounters one or more lysis agents and/or mucolytic agents. The mucolytic agent is preferably pre-loaded onto the collection device. In a preferred embodiment, the mucolytic agent is localized between the sample application zone and the conjugate zone. In embodiments with a sample compressor, one or more mucolytic agents may be pre-loaded and dried on the sample compressor, the sample collector, in various locations on the test strip, or in the running buffer. | 2012-10-11 |
20120258470 | COMPOSITIONS COMPRISING A DEHALOGENASE SUBSTRATE AND A RADIONUCLIDE AND METHODS OF USE - A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein. | 2012-10-11 |
20120258471 | PEPTIDE MARKER FOR CANCER DIAGNOSIS, AND CANCER DIAGNOSIS METHOD USING SAME - The present invention relates to a peptide marker for cancer diagnosis and a cancer diagnosis method using the same, more precisely to a peptide marker for cancer diagnosis screened by the following steps: separating and concentrating glycoproteins including a certain glycan chain related to the occurrence of cancer; hydrolyzing the glycoproteins to obtain polypeptides; and quantitatively analyzing the polypeptides to identify certain polypeptides that can track quantitative changes in glycoproteins glycosylated in a specific manner by the occurrence of cancer, and to a cancer diagnosis method using the said peptide marker. | 2012-10-11 |
20120258472 | SYSTEMS AND METHODS OF FLUIDIC SAMPLE PROCESSING - The present invention provides fluidic devices and systems that allow detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications. | 2012-10-11 |
20120258473 | METHOD OF COLLECTING SPECIMEN AND METHOD OF DIAGNOSING SUBJECT TO DETECT UPPER DIGESTIVE SYSTEM DISEASE - A method of collecting a specimen of the present invention is used in detecting upper digestive system disease. The method of collecting a specimen includes: a step of positioning sampling equipment in the duodenum of the subject into which duodenal juice is secreted, the sampling equipment being used to collect and store the duodenal juice; a step of collecting duodenal juice naturally secreted in the duodenum using the sampling equipment; and a step of stopping collection of the duodenal juice when collection quantity of the duodenal juice reaches a predetermined quantity which is 3.0 ml or less. | 2012-10-11 |
20120258474 | METHODS OF DETECTING DNA DAMAGE - The present invention relates to methods of detecting agents that cause or may potentiate DNA damage, and to assays that may be employed in such methods. In particular, the invention relates to methods of detecting DNA damage in in vitro cultures of human cells. | 2012-10-11 |
20120258475 | RECOVERY OF RARE CELLS USING A MICROCHANNEL APPARATUS WITH PATTERNED POSTS - A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption. | 2012-10-11 |
20120258476 | METHODS FOR INDUCING A NATURAL KILLER (NK) CELL-MEDIATED IMMUNE RESPONSE AND FOR INCREASING NK CELL ACTIVITY - This disclosure relates to methods of inducing a natural killer (NK) cell-mediated immune response and increasing NK activity in a mammal for the treatment of tumors and virus infections, comprising isolating peripheral blood mononuclear cells (PBMCs) from a subject, exposing them in vitro to a protein conjugate comprising granulocyte macrophage colony stimulating factor (GM-CSF) covalently linked to a soluble peptide antigen to activate the PBMCs, and administering the activated PBMCs to the subject. The method also relates to assessing NK cell activity of activated PBMCs to determine whether the subject has had a therapeutically effective response to the protein conjugate. | 2012-10-11 |
20120258477 | FECAL NEOPTERIN CONCENTRATION MEASUREMENT AS AN INDICATOR OF DISEASE ACTIVITY IN INFLAMMATORY BOWEL DISEASE - Disclosed are methods for determining disease activity in a patient having or at risk for developing inflammatory bowel disease (IBD) which include measuring neopterin concentration in a fecal sample from the patient. | 2012-10-11 |
20120258478 | METHOD OF COLLECTING SPECIMEN AND METHOD OF DIAGNOSING SUBJECT TO DETECT UPPER DIGESTIVE SYSTEM DISEASE - A method of collecting a specimen of the present invention detects upper digestive system disease. The method of collecting a specimen includes: a step of administering a stimulant of pancreatic juice secretion or a stimulant of bile secretion to a subject; a step of positioning sampling equipment in the duodenum of the subject into which duodenal juice is secreted, the sampling equipment being used to collect and store the duodenal juice; a step of starting collection of the duodenal juice by the sampling equipment within five minutes from the administration of the stimulant of pancreatic juice secretion or the stimulant of bile secretion; and a step of stopping collection of the duodenal juice such that collection time for the duodenal juice is within five minutes from the start of collection. | 2012-10-11 |
20120258479 | Assay Device Having Rhombus-Shaped Projections - An assay device includes: a liquid sample receiving zone; a capture zone in fluid communication with the sample receiving zone having capture elements bound thereto, the capture zone having a substrate and projections which extend substantially vertically from the substrate, the projections having a rhombus-shaped cross-section and the projections arranged on the substrate with the corners of the projections facing upstream in a direction toward the sample receiving zone. The projections have a height, cross-section and a distance between one another that defines a capillary space between the projections capable of generating capillary flow parallel to the substrate surface; and a sink in fluid communication with the capture zone having a capacity to receive liquid sample flowing from the capture zone. The sample receiving zone, the capture zone and the sink define a fluid flow path. Preferably, the rhombus-shaped cross-section is a square or a diamond. | 2012-10-11 |
20120258480 | ADP DETECTION BASED LUMINESCENT PHOSPHOTRANSFERASE OR ATP HYDROLASE ASSAY - The invention provides provides compositions and methods to determine or detect the activity of enzymes, including phosphotransferases such as kinases (e.g., protein, lipid, and sugar kinases) and ATP hydrolases such as ATPases, e.g., HSP90, that employ ATP as a substrate and form ADP as a product by monitoring changes in ADP. | 2012-10-11 |
20120258481 | CROSS-LINKING ATP ANALOGS - An adenosine 5′-triphosphate analogs modified at the gamma-phosphate with a reactive reagent. A method of forming the analog by activating a 4-amino benzoic acid, incubating the activated acid to obtain an amine, and coupling the amine with ATP in the presence of water soluble EDCI. A method of detecting the efficacy of a therapeutic by adding a gamma-phosphate modified ATP analog to a protein substrate, reacting the target proteins with the ATP analog, and analyzing the resulting cross-linked product, wherein the amount of product present correlates to the efficacy of the therapeutic is also provided. | 2012-10-11 |
20120258482 | PCNA METHYLTRANSFERASE - Proliferating cell nuclear antigen (PCNA)-dependent glutamate methyltransferases are disclosed that can methylesterify one or more glutamic acid or aspartic acid residues of PCNA. | 2012-10-11 |
20120258483 | METHOD FOR DETECTING A WOUND INFECTION - The present invention relates to a method for detecting a wound infection comprising the steps of: contacting a sample obtained from a wound with at least two substrates for at least two enzymes selected from the group consisting of lysozyme, elastase, cathepsin G and myeloperoxidase, and detecting a wound infection when a conversion of the at least two substrates with said at least two enzymes are determined. | 2012-10-11 |
20120258484 | CANCER DIAGNOSIS MARKER USING THE ABERRANT GLYCOSYLATION OF A PROTEIN - The present invention relates to a method for diagnosing cancer using information on the aberrant glycosylation of a glycoprotein. More precisely, the present invention relates to a cancer diagnosis peptide marker, which is screened by: a step of separating, using a lectin, a glycoprotein which is aberrantly glycosylated in accordance with the occurrence of cancer; and a step of selecting a hydrolyzed peptide marker derived from the aberrantly glycosylated glycoprotein by observing the quantitative changes in the separated glycoprotein. The present invention also relates to a method for diagnosing cancer using the peptide marker as an agent. | 2012-10-11 |
20120258485 | METHOD FOR DETECTING AND QUANTIFYING A TARGET MOLECULE IN A SAMPLE - The invention relates to a method for identifying and quantifying by mass spectrometry at least one target molecule in a sample, comprising the following steps:
| 2012-10-11 |
20120258486 | CALCIUM-BINDING PHOTOPROTEIN, GENE ENCODING THE SAME, AND USE THEREOF - A protein according to the invention can be used to detect or measure calcium ions is provided. Further the protein is useful as a reporter protein or a luminescence marker. A polynucleotide according to the invention is also useful as a reporter gene. | 2012-10-11 |
20120258487 | MICROFLUIDIC PLATFORM FOR SYNTHETIC BIOLOGY APPLICATIONS - This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of said biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to for a sequenced order of droplets carrying different components of said biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of said biological construct to permit said components to react with each other in one or more reactions contributing to the assembly of said biological construct. | 2012-10-11 |
20120258488 | Systems and Methods for Electrophysiological Activated Cell Sorting and Cytometry - Provided herein are methods and systems for non-genetic, label-free cell purification (i.e., cell cytometry and sorting), which classifies cells based on their spontaneous electrophysiological response or their electrophysiological response to a stimulus. For example, in one embodiment, there is provided a method of cell sorting comprising: stimulating a cell with a stimulus; sensing a response evoked by the cell based on the stimulus; identifying a phenotype of the cell based on the evoked response; and sorting the cell based on its phenotype. In one embodiment, the stimulus may be an electrical stimulus, a mechanical stimulus, an optical stimulus, a thermal stimulus, a chemical stimulus, or any combination thereof. The cell phenotype may be, for example, cardiomyocytes, neurons, smooth muscle cells, or pancreatic beta cells. | 2012-10-11 |
20120258489 | FUNGAL PROMOTERS FOR EXPRESSING A GENE IN A FUNGAL CELL - The present invention relates to isolated fungal promoter DNA sequences, to DNA constructs, vectors, and fungal host cells comprising these promoters in operative association with coding sequences encoding polypeptides. The present invention also relates to methods for expressing a gene and/or producing a polypeptide using the new promoters isolated. The present invention also relates to methods for altering the transcription level and/or regulation of an endogenous gene using the new promoter of the invention. | 2012-10-11 |
20120258490 | PROCESS FOR PRODUCING DIPEPTIDES OR DIPEPTIDE DERIVATIVES - The present invention provides a process for producing a dipeptide or a dipeptide derivative using a phosphate donor, a substance selected from the group consisting of adenosine-5′-monophosphate, adenosine-5′-diphosphate and adenosine-5′-triphosphate, one or more kinds of amino acids or amino acid derivatives, and as enzyme sources, a protein having polyphosphate kinase activity, or a culture of cells having the ability to produce the protein or a treated matter of the culture, and a protein having the activity to ATP-dependently form the dipeptide or dipeptide derivative from one or more kinds of amino acids or amino acid derivatives, or a culture of cells having the ability to produce the protein or a treated matter of the culture. | 2012-10-11 |
20120258491 | EXPRESSION OF BIOLOGICALLY ACTIVE POLYPEPTIDES IN DUCKWEED - Methods, nucleic acid sequences, and transformed duckweed plant or duckweed nodule cultures for the expression and the secretion of biologically active polypeptides from genetically engineered duckweed are provided. Expression of recombinant polypeptides in duckweed is improved by modifying the nucleotide sequence of the expression cassette encoding the polypeptide for improved expression in duckweed. Recovery of biologically active polypeptides from duckweed is improved by linking the biologically active polypeptide to a signal peptide that directs the secretion of the polypeptide into the culture medium. | 2012-10-11 |
20120258492 | BACTERIAL HOST STRAIN - A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a) a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b) a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c) a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated DegP gene and optionally a polynucleotide sequence encoding a protein of interest. | 2012-10-11 |
20120258493 | PROMOTER SEQUENCES - The present invention relates to new promoter sequences and uses thereof, in particular expression cassettes, vectors, and methods of expressing genes using the new promoters. | 2012-10-11 |
20120258494 | Retroviral Vector Particles and Methods for their Generation and Use - The present invention relates to methods of host cell transduction utilising ecotropic retroviral vector particles. The retroviral vector particle may comprise an envelope of Friend murine leukaemia virus, in particular the envelope encoded by molecular clone PVC-211 and the host cell may be engineered to recombinantly express the Reel receptor. The retroviral vector particles and methods of the invention can be used to introduce expressible polynucleotide sequences of interest into host cells with high efficiency. This results in protein production methods with higher yield (mg/L) and a reduction in manufacturing costs that could be used in a range of applications including for example, the production of therapeutic proteins, vaccines and antibodies. | 2012-10-11 |