41st week of 2008 patent applcation highlights part 38 |
Patent application number | Title | Published |
20080248502 | Methods For Detecting Th1 Cells - The inventors discovered that the adhesion molecule CAR, known to be localized in intracellular adhesion sites, functioned as an adhesion molecule for activated lymphocytes. Further, the inventors identified CARL, a novel CAR ligand expressed in lymphocytes, and clarified that the ligand was expressed selectively in Th1 cells. In addition, they found that anti-CAR antibodies could inhibit the adhesion of activated lymphocytes to CAR molecules. Thus, the present invention provides methods for detecting Th1 cells using CAR or anti-CARL antibodies, and methods of screening for inhibitors suppressing the adhesion of Th1 cells using the binding between CAR and CARL as an index. Furthermore, the present invention relates to methods of screening for inhibitors of the binding between CAR and CARL, antibodies that inhibit the binding between CAR and CARL, and therapeutic compositions comprising these antibodies. These are expected to be useful in diagnosing diseases, such as inflammation, in which infiltration of Th1 cells is involved, and in providing pharmaceutical agents for alleviating such diseases. | 2008-10-09 |
20080248503 | Methods of Screening Compounds to Predict Toxicity and Residual Proliferative and Differentiation Capacity of the Lympho-Hematopoietic System - The present invention relates generally to kits that provide reagent mixes and instructions for the use thereof, in performing high-throughput assay methods that provide a method of screening compounds for cytotoxicity or other effects on target cell populations of the lymphohematopoietic system, including specific lineages. The methods measure the luminescent output derived from the intracellular ATP content of incubated target cells, and correlate the luminescence with the proliferative status of the cells. The methods may be used to predict the effect of virtually any compound on the lymphohematopoietic system and may be performed on multiple species simultaneously, thereby providing valuable information regarding potential cytotoxicity prior to preclinical studies and especially, patient clinical trials. The methods also provide the ability to screen compounds early in the drug development profile. | 2008-10-09 |
20080248504 | Agglutination Assay - The invention relates to agglutination assays and related kits, reagents and devices. In particular methods of assaying small analytes having few epitopes are disclosed, by means of using hub moieties to which multiple analytes may be bound by a first epitope, together with a further moiety capable of binding a second analyte epitope and which is also capable of binding to a detectable particle. Stable agglutinated complexes may be so formed, which may used as the basis for various assay formats. | 2008-10-09 |
20080248505 | Detection of Lipid Oxidising Abzymes in Samples - This invention relates to the finding that lipid oxidising abzymes damage | 2008-10-09 |
20080248506 | Method of Monitoring Anti-Tumor Activity of an Hdac Inhibitor - The present invention relates to the method of determining the anti-tumor activity of a histone deacetylase inhibitor by measuring the phosphorylation of the histone variant H2AX or the level of cytokeratin-18 fragment aa 387-397. | 2008-10-09 |
20080248507 | C-erbB-2 external domain: GP75 - Disclosed are methods and compositions for identifying malignant tumors that overexpress the c-erbB-2 oncogene. Assays useful for diagnosis and prognosis of neoplastic disease are provided which detect the external domain of c-erbB-2, the glycoprotein gp75 and quantitate the level of gp75 in the biological fluids of mammals carrying a tumor burden. | 2008-10-09 |
20080248508 | Methods of making a chitosan product having an ultra-low endotoxin concentration and the ultra-low endotoxin chitosan product derived therefrom and method of accurately determining inflammatory and anti-inflammatory cellular response to such materials - Chitosan is a natural product having wide range of applications in the food and cosmetic industries. Food and commodity grade chitosan are laden with pyrogens, such as endotoxins and proteins which limit its applicability in the biological and medical arenas, as minute amounts of endotoxins may induce adaptive and innate responses when contacted with mammalian tissue, pharmaceuticals and biomedical devices. Due to chitosan's ability to avidly bind endotoxin and other pyrogens, they are difficult to remove. The present invention is directed to methods for purifying chitosan from shells, food and commodity grade chitosan into ultra-pure, low endotoxin chitosan having biological and medical applicability. Additionally, the present invention is also directed to a method of determining the pyrogenicity of the ultra-pure low endotoxin chitosan. | 2008-10-09 |
20080248509 | METHOD FOR DIAGNOSIS OF ALZHEIMER'S DISEASE WITH DETERMINATION OF LASP-1 IMMUNOREACTIVITY - Method for early diagnosis and diagnosis, for prognosis and assessment of the severity and for therapy-accompanying monitoring of inflammatory diseases and infections, in particular sepsis-like systemic infections and Alzheimer's disease, in which the presence and/or amount of the protein LASP-1 (SEQ ID NO:1) or of the protein LAP-1 (SEQ ID NO:16) or of an immunoreactive fragment of one of these proteins in free and/or protein-bound form is determined, preferably as immunoreactivity, in a biological fluid, or optionally a tissue sample, of a patient, and conclusions are drawn with respect to the presence, the expected course, the severity or the success of a therapy of the inflammatory disease or of the infection from the presence and/or amount of the proteins determined. | 2008-10-09 |
20080248510 | HUMAN Fc GAMMA RECEPTOR III - The present invention relates to the field of human immunoglobulin receptors, specifically the glycostructure of a human Fc gamma receptor IIIa recombinantly expressed in human embryonic kidney cells and Chinese hamster ovary cells. | 2008-10-09 |
20080248511 | METHODS TO QUENCH LIGHT FROM OPTICAL REACTIONS - The present invention relates to single and dual reporter luminescence assays utilizing reagents to quench an optical, e.g., an enzyme-mediated luminescence, reaction. In one embodiment of the invention, a reagent is added to an assay which selectively quenches a first enzyme-mediated luminescence reaction without affecting a subsequent distinct enzyme-mediated luminescent reaction(s). An assay kit containing one or more selective quench reagents, and compositions comprising the quench reagent(s), are also provided. | 2008-10-09 |
20080248512 | Methods for detection of lysosomal storage disease - The present invention provides compositions for performing assays of enzyme activity associated with lysosomal storage diseases. The invention further provides methods for determining enzyme activity, and methods for the screening for lysosomal storage disease in an individual. | 2008-10-09 |
20080248513 | Methods for detection of lysosomal storage disease - The present invention provides compositions for performing assays of enzyme activity associated with lysosomal storage diseases. The invention further provides methods for determining enzyme activity, and methods for the screening for lysosomal storage disease in an individual. | 2008-10-09 |
20080248514 | ELECTROCHEMICAL METHOD FOR GLUCOSE QUANTIFICATION, GLUCOSE DEHYDROGENASE COMPOSITION, AND ELECTROCHEMICAL SENSOR FOR GLUCOSE MEASUREMENT - A method of quantifying glucose in a solution characterized in that electric potential measurement is conducted by potentiometry using a glucose dehydrogenase that requires a flavin compound as a coenzyme. It is preferable to carry out the quantification using a glucose dehydrogenase derived from a filamentous fungus, in particular derived from | 2008-10-09 |
20080248515 | Optimizing culture medium for CD34<+> hematopoietic cell expansion - The present invention provides a method of determining the optimal composition of a serum-free, eukaryotic cell culture medium supplement, using 2-level factorial design and the deepest ascent method. The invention further provides a method of making a serum-free eukaryotic cell culture medium supplement and the generated thereof. The invention further provides a method of making a serum-free, eukaryotic cell culture medium and the medium generated thereof. The invention further provides a kit containing the medium of the invention. The invention also provides a method of expanding CD34<+> hematopoietic cells and a composition comprising CD34<+> hematopoietic cells in a serum-free, eukaryotic cell culture medium of the invention. | 2008-10-09 |
20080248516 | Method for Using Division Arrested Cells in Screening Assays - Division arrested cells are used in screening assays to determine the effect of a substance of interest on the cells. The division arrested cells can be used in drug screening assays, signal transduction assays, and are especially useful in large scale, high throughput assays. | 2008-10-09 |
20080248517 | Diagnostic Device - Diagnostic devices for detecting the presence of an analyte in a sample are provided. Devices of the present invention comprise a means for inducing a pressure differential on a sample to direct the sample to a test surface. In one embodiment, the means for inducing a pressure differential on a sample to direct the sample to a test surface comprises a syringe that can be used to draw a sample from an opening to a test surface. In other embodiments, the device also provides means for diluting a sample. In yet other embodiments, the device also provides a means | 2008-10-09 |
20080248518 | E.coli and salmonella test kit - A method for testing for the presence of bacteria in the field includes using a mixer and frother or a reagent to disrupt bacteria cells. A sample is collected from a source suspected to be contaminated by bacteria. A mixer and frother or similar mixer is used to mix the sample, or a reagent is added to the sample, to form an elute by disrupting bacteria cells present in the sample to expose bacteria enzymes. A reagent pad is immersed in the elute and then withdrawn from the elute, or a drop of the elute is deposited on a reagent pad. After a waiting period, the reagent pad is observed to determine if the bacteria is present in or on the source. The source may be a body of water, produce, prepared food, a food preparation area, or food processing equipment. The reagent is generally a lysis reagent. | 2008-10-09 |
20080248519 | Agitator For A Fermenter, Fermenter And Method For Operating A Fermenter - The invention relates to an agitator for a fermenter, a fermenter and method of operating a fermenter. | 2008-10-09 |
20080248520 | Process for Production of 5-Ene-3-One or 3,6-Dione Derivatives of Sterols, Processes for Production of Lipid Metabolism Improvers, Foods, Drinks, and Animal Feeds, and Analytical Method - An object of the present invention is to provide a process according to which 5-ene-3-one or 3,6-dione derivatives of sterols can be synthesized with a better yield, as well as a process for production of lipid metabolism improvers, foods, drinks, and animal feeds containing the 5-ene-3-one or 3,6-dione derivatives of sterols. | 2008-10-09 |
20080248521 | ENHANCED CELL-FREE SYNTHESIS OF ACTIVE PROTEINS CONTAINING DISULFIDE BONDS - Compositions and methods are provided for the enhanced in vitro synthesis of active polypeptides containing disulfide bonds. In certain embodiments of the invention, the reaction mix includes a biological extract derived from a bacterial cell in which the glutathione reductase gene has been inactivated, which is pre-treated with a low concentration of a sulfhydryl inactivating agent. | 2008-10-09 |
20080248522 | HIGH-PRESSURE INCLUSION BODY SOLUBILIZATION AND PROTEASE CLIPPING OF RECOMBINANT FUSION PROTEINS - Described herein are methods for the solubilization and proteolytic cleavage of fusion protein aggregates, including autocatalytic fusion proteins, at pressures greater than atmospheric pressure to yield soluble target polypeptides. | 2008-10-09 |
20080248523 | Utilisation of Constructs Comprising Recombination Sequence Motifs for Enhancing Gene Expression in Moss - A method of amplifying gene expression in a moss plant cell or moss tissue, DNA constructs therefor, moss plant cells and uses thereof for the production of protein. | 2008-10-09 |
20080248524 | AMP Deaminase Originating Streptomyces And Utilization Thereof - It is intended to provide a thermostable AMP deaminase originating in a microorganism. Namely, an AMP deaminase having the following characteristics. (1) Catalyzing the reaction: 5′-adenylic acid+H | 2008-10-09 |
20080248525 | Twin-Arginine translocation in bacillus - Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a | 2008-10-09 |
20080248526 | Recombinant Cell Lines for the Stable and High-Level Production of Biologically Active Dkk1 Protein - This invention relates to the use of specific recombinant cell lines in order to produce biologically active DKK1 protein in a stable and high-level manner. The invention also relates to these recombinant cell lines, as well as a method for stable and high-level production of biologically active DKK1 protein, implementing such recombinant cell lines. | 2008-10-09 |
20080248527 | Binding Phenol Oxidizing Enzyme-Peptide Complexes - The present application relates to peptides which bind to a target stain, phenol oxidizing enzyme-binding peptide complexes wherein the binding peptide is attached to the C-terminus of the phenol oxidizing enzyme or is inserted or substituted into the phenol oxidizing enzyme. In a preferred embodiment the phenol oxidizing enzyme is a laccase specifically | 2008-10-09 |
20080248528 | ERYTHROVIRUS AND ITS APPLICATIONS - The invention relates to nucleic sequences derived from a human erythrovirus type V9, fragments of the sequences and their methods of use including applications as a diagnostic reagent and as immunogenic agent. | 2008-10-09 |
20080248529 | MODIFIED ANTI-CD52 ANTIBODY - The present invention provides for modified forms of anti-CD52 antibodies with reduced numbers of potential T-cell epitopes that are expected to display decreased immunogenicity. | 2008-10-09 |
20080248530 | Use of Fungal Mutants for Expression of Antibodies - The present invention relates to a filamentous fungal cell in which an endogenous alkaline protease activity, an endogenous neutral metalloprotease activity and an endogenous serine protease activity of the subtilisin type have been completely or partially inactivated. Particularly the endogenous alkaline protease activity, the endogenous neutral metalloprotease activity and the endogenous serine protease activity of the subtilisin type are encoded by the alp, npI and pepC genes respectively. The filamentous fungal cell is particularly suitable for production of heterologous proteins such as antibodies. | 2008-10-09 |
20080248531 | Preparation of fully human antibodies - The present invention provides a method of preparing fully human antibodies that recognize a pre-determined antigen without relying on human donors that have already been exposed to the antigen. To this end, lymphocytes from naive human donors are immunized in vitro with the antigen of interest, and cells that produce antibodies against the antigen are identified. Since the lymphocytes are immunized in vitro rather than in vivo, it is possible to control which antigen, or which part of the antigen, would be recognized by the antibody. A preferred antigen is gp120 of HIV, particularly the co-receptor binding region of gp120. | 2008-10-09 |
20080248532 | Enzymatic Method for Producing Dihydroxyacetone Phosphate - A method for making dihydroxyacetone phosphate (DHAP), comprising treating dihydroxyacetone with a bacterial acid phosphatase in the presence of pyrophosphate. The invention further pertains to a method for stereospecifically making, preferably in one pot, a compound of the formula: | 2008-10-09 |
20080248533 | METHOD FOR IN VITRO MOLECULAR EVOLUTION OF PROTEIN FUNCTION - The invention provides a method for generating a polynucleotide sequence or population of sequences from parent single stranded polynucleotide sequences encoding one or more protein motifs, comprising the steps of
| 2008-10-09 |
20080248534 | Adaptive Thermal Block Temperature Control Method and System - Aspects of the present teachings describe a method and apparatus for automatically controlling a block temperature to reduce undershooting and overshooting of the temperatures of a sample contained in the block and participating in a polymerase chain reaction (PCR). The adaptive thermal block temperature control begins when a sample temperature enters a sample window region between a preliminary setpoint temperature and a target setpoint temperature for the sample. Based on thermodynamic behavior of the sample and the predetermined phase of PCR, predicting a time period measured subsequent to the preliminary setpoint temperature when the sample will reach the target setpoint suitable for the predetermined phase of PCR. During this time period, varying the block temperature ramp rate with a series of cooling and heating changes to ensure the block temperature reaches the target setpoint temperature at approximately the same time as the sample reaches the same. Synchronizing the block temperature and sample temperature to the target setpoint temperature reduces undershooting and overshooting of the sample temperature and increases the speed and efficiency of the overall PCR process as it relates to the thermal cycling operations. | 2008-10-09 |
20080248535 | RAPID ONE-STEP REVERSE TRANSCRIPTASE PCR - The present invention is directed to a method for performing a one-step RT-PCR for amplifying a target RNA comprising the steps (i) providing a sample which is supposed to contain said target RNA (ii) adding a reaction mixture comprising all reagents necessary to reverse transcribe said target RNA into cDNA and amplify at least a portion of said cDNA (iii) incubating said sample for a time interval of 0 seconds to 40 seconds at a temperature between 20° C. and 65° C., and (iv) subjecting said sample to multiple cycles of a thermocycling protocol wherein the temperature of said sample is varied between at least a first temperature between 37° C. and 72° C. and a second temperature between 85° C. and 100° C. | 2008-10-09 |
20080248536 | Polynucleotide Ligation Reactions - The method of the invention improves the specificity of a ligation reaction carried out between a first double-stranded polynucleotide having a single-stranded portion and a second polynucleotide having a complementary single-stranded portion, the second polynucleotide being present in a sample comprising a mixture of different polynucleotides. The method comprises contacting the sample, under hybridising conditions, with the first polynucleotide and one or more third polynucleotide(s) wherein the third polynucleotide(s) comprises a single-stranded portion that differs from the single-stranded portion of the first polynucleotide by at least one base substitution. | 2008-10-09 |
20080248537 | BIOCATALYTIC ASYMMETRIC REDUCTION IN PREPARATION OF (S)-N-[5-(1,2-DIHYDROXY-ETHYL)-PYRAZINYL]-2,2-DIMETHYL-PROPIONAMIDE - The present invention relates to biocatalytic asymmetric reduction for the preparation of 2-amino-[5-(1-hydroxy-2-hydroxy or halogen-ethyl)]-pyrazine derivatives of the formula | 2008-10-09 |
20080248538 | Cyanide-Tolerant Nitrile Hydratases - Disclosed herein are cyanide-tolerant nitrile hydratases especially from | 2008-10-09 |
20080248539 | COMPOSITIONS AND METHODS FOR PRODUCING STEREOISOMERICALLY PURE STATINS AND SYNTHETIC INTERMEDIATES THEREFOR - The present disclosure provides ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, method of using the engineered ketoreductase enzymes to synthesize a variety of chirally pure compounds, and the chirally pure compounds prepared therewith. | 2008-10-09 |
20080248540 | METHODS OF PRODUCING BUTANOL - A method of producing butanol from carbohydrates is provided. A feedstock comprising a carbohydrate source is fermented in the presence of bacteria to produce butyric acid and hydrogen. The butyric acid is then hydrogenated in the presence of a catalyst to produce butanol. | 2008-10-09 |
20080248541 | Equipment and Method for Dry Ferment - The present invention relates to a method for dry fermentation and equipment for carrying out the same. The invention equipment comprises a fermentation tank for dry fermentation which includes a tank body | 2008-10-09 |
20080248542 | Clostridium difficile culture and toxin production methods - The invention provides methods and compositions for use in culturing | 2008-10-09 |
20080248543 | Method For Removing Enzyme and Method of Base Exchange or Hydrolysis of Phospholipid Using the Same - A method of removing an enzyme from a liquid enzyme reaction mixture used in a hydrolysis reaction or a base exchange reaction of a phospholipid is provided. The method includes the step of treating the liquid enzyme reaction mixture with a solvent mixture of water and an organic solvent, wherein the solvent mixture includes an inorganic metal salt, to remove the enzyme. Enzymes included in the reaction product can be easily removed without a treatment such as heating, and thus it becomes possible to easily produce various phospholipids that have a reduced risk of inducing an allergy, that retain a high quality and that have excellent storage stability. | 2008-10-09 |
20080248544 | Methods And Compositions For Grafting Functional Loops Into A Protein - The present invention provides targeted enzymes that bind to targets better than the corresponding pre-targeted enzymes bind the target under like conditions, methods of making targeted enzymes, methods of using targeted enzymes to treat diseases, and pharmaceutical compositions comprising targeted enzymes. | 2008-10-09 |
20080248545 | Methods for Generating Novel Stabilized Proteins - The disclosure provides methods for identifying and producing stabilized chimeric proteins. | 2008-10-09 |
20080248546 | Mutant 2,5-diketo-L-gluconic acid reductases - Described herein are novel nucleic acids, proteins and methods that can be used to provide new catalysts with desirable traits for industrial processes. In particular, novel reductases isolated from the environment using PCR methods are described. | 2008-10-09 |
20080248547 | Novel glyphosate-N-acetyltransferase (GAT) genes - Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate. | 2008-10-09 |
20080248548 | MODULATION OF PROTEIN FUNCTIONALITIES - New methods for the rational identification of molecules capable of interacting with specific naturally occurring proteins are provided, in order to yield new pharmacologically important compounds and treatment modalities. Broadly, the method comprises the steps of identifying a switch control ligand forming a part of a particular protein of interest, and also identifying a complemental switch control pocket forming a part of the protein and which interacts with said switch control ligand. The ligand interacts in vivo with the pocket to regulate the conformation and biological activity of the protein such that the protein assumes a first conformation and a first biological activity upon the ligand-pocket interaction, and assumes a second, different conformation and biological activity in the absence of the ligand-pocket interaction. Next, respective samples of said protein in the first and second conformations are provided, and these are screened against one or more candidate molecules by contacting the molecules and the samples. Thereupon, small molecules which bind with the protein at the region of the pocket may be identified. Novel protein-modulator adducts and methods of altering protein activity are also provided. | 2008-10-09 |
20080248549 | GLUTATHIONE S-TRANSFERASE SEQUENCE VARIANTS - Isolated GSTO2 nucleic acid molecules that include a nucleotide sequence variant and nucleotides flanking the sequence variant are described, as well as GSTO2 allozymes. Methods for determining if a subject contains a GSTO2 sequence variant also are described. | 2008-10-09 |
20080248550 | ENHANCEMENT OF VANADIUM-CONTAINING PHOSPHATASE INHIBITORS - The present invention is directed to a composition comprising a vanadium-containing phosphatase inhibitor and a polyol. In the presence of the polyol the effect of the inhibitor is enhanced, even in the presence of chelating agents or reducing agents. The invention also concerns the use of the inventive composition for inhibiting a phosphatase, as well as kits comprising the composition. | 2008-10-09 |
20080248551 | METHODS AND COMPOSITIONS FOR LIVE ATTENUATED VIRUSES - Embodiments herein relate to compositions of and methods for live viruses. In certain embodiments, a live, attenuated virus composition includes, but is not limited to, one or more live, attenuated viruses and compositions to reduce inactivation and/or degradation of the live, attenuated virus. In other embodiments, the live, attenuated virus composition may be a vaccine composition. In yet other compositions, a live, attenuated virus composition may include at least one carbohydrate, at least one protein and at least one high molecular weight surfactants for reducing inactivation and/or degradation of the live, attenuated virus. | 2008-10-09 |
20080248552 | METHOD OF CELL CULTURES AND DEVICE FOR IMPLEMENTING IT - The invention generally provides a cell culture vessel having at least one first zone and at least one second zone, wherein the first zone is a transfer zone for a culture medium which essentially contains no cells and the second zone is a cell culture zone. The invention further includes methods utilizing the cell culture vessel. | 2008-10-09 |
20080248553 | Production of Bioavailable Folic Acid - The invention provides a process of producing bio-available folate, i.e., folic acid having an increased proportion of monoglutamyl folate and a decreased proportion of polyglutamyl folate, by culturing food-grade microorganisms containing an active heterologous or homologous polyglutamyl hydrolase activity or containing increased activities of folate biosynthesis enzymes. The genes encoding the polyglutamyl hydrolase and the folate biosynthesis enzymes may be of various origin, e.g. from rodents or other mammals including man. Also provided is a foodstuff, especially a dairy product containing such monoglutamyl folate-producing microorganisms. | 2008-10-09 |
20080248554 | DEVICES AND METHODS FOR SELECTIVELY LYSING CELLS - A device for generating microbubbles in a gas and liquid mixture and injection device, the device comprising: a housing defining a mixing chamber; means for mixing solution contained in the mixing chamber to generate microbubbles in the solution; a needle array removably attached to the housing and in fluid connection with the mixing chamber, the needle array including at least one needle; and a machine readable identifier on the needle array. | 2008-10-09 |
20080248555 | Bacterial Consortium Nbc2000 and Method for Biologically Treating Endocrine Disrupters Using the Nbc2000 - Disclosed is a novel bacterial consortium comprise bacterial strains useful for effectively treating endocrine disrupters and a method for treating endocrine disrupters using the same. The invention provides a method for biologically restoring soils, wastes and water, etc. which are polluted with chlorinated compounds such as polychlorinated biphenyl (PCBs), dioxin, pentachlorophenol (PCP), perchloroethylene (PCE), trichloroethylene (TCE) and 1,1,1-trichloroethane (1,1,1-TCA), etc., polycyclic aromatic hydrocarbons (PAH) and petroleum-tar acids, and toluene which are all known as representative endocrine disrupters. | 2008-10-09 |
20080248556 | USE OF SONICATION TO ELIMINATE PRIONS - The present disclosure generally relates to methods for disinfecting surfaces. More particularly, the present disclosure relates to methods for destroying prion molecules using a combination of ultrasonic energy and enzyme treatment that is effective to denature and degrade prion proteins. | 2008-10-09 |
20080248557 | Method of Detoxication of Yperite by Using Haloalkane Dehalogenases - The method of detoxification of yperite—bis(2-chloroethyl)sulfide—by the use of a haloalkane dehalogenases or their compositions, the method of preparation of dehalogenationating enzymes and of decontamination compositions which contains at least one wild type and/or modified haloalkane dehalogenase (EC 3.8.1.5) as an chemically active component. The preferred dehalogenases are LinB from | 2008-10-09 |
20080248558 | Methods For Preventing, Removing, Reducing, or Disrupting Biofilm - The present invention relates to methods for preventing, removing, reducing, or disrupting biofilm present on a surface, comprising contacting the surface with an alpha-amylase derived from a bacterium. | 2008-10-09 |
20080248559 | Method For Selectively Separating and Purifying Rna and Method For Separating and Purifying Nucleic Acid - A method for selectively separating and purifying RNA from a mixture solution of nucleic acid containing DNA and RNA, wherein the method comprising the steps of: (1-a) adsorbing nucleic acid; (1-b) washing; (1-c) subjecting to a DNase treatment; (1-d) washing; and (1-e) desorbing the RNA from a nucleic acid-adsorbing porous membrane by a recovering solution, wherein in the step (1-c), a total amount of a DNase solution is 130 μl or less per 1 cm | 2008-10-09 |
20080248560 | Method of Treatment of Tissue Processing Fluid and Apparatus Therefor - The present invention relates to the field of tissue processing, particularly, to the treatment of fluids used in tissue processing. In one embodiment the present invention provides for treating a tissue processing fluid comprising first processing fluid comprising a first processing fluid and a second contaminating fluid, by: gradually varying the pressure of the fluid from an initial pressure level to a first pressure level such that the fluid remains below a vaporizing point of the second contaminating fluid and, heating the fluid to at least a vaporizing temperature of the second contaminating fluid. In another embodiment, the present invention removes the second contaminating fluid from the fluid by varying at least one of the pressure and temperature of the fluid so as to allow the second contaminating fluid to vaporize. Alternatively, an embodiment of the present invention interleaves the step of removing with one or more tissue processing protocol steps. In a preferred embodiment, the invention relates to the treatment of infiltrating materials, particularly in their liquid form in a histological tissue sample processor capable of Xylene free operation. | 2008-10-09 |
20080248561 | Molecular characterization with carbon nanotube control - There is provided a first reservoir containing a liquid solution including a molecule to be characterized and a second reservoir for containing a liquid solution including a molecule that has been characterized. A solid state support structure is provided including an aperture having a molecular entrance providing a fluidic connection to the first reservoir and a molecular exit providing a fluidic connection to the second reservoir. One carbon nanotube is provided having a longitudinal sidewall disposed as a molecular contacting surface at the aperture. A voltage source is connected in series with the carbon nanotube for electrically biasing the carbon nanotube, and an electrical current monitor is connected in series with the carbon nanotube for monitoring changes in electrical current through the nanotube corresponding to translocation of a molecule through the aperture. | 2008-10-09 |
20080248562 | Microorganism Separation Device - A microorganism separation device includes: sample solution supply means ( | 2008-10-09 |
20080248563 | Specimen container for the micro manipulation and biopsy in in-vitro fertilization - A container or dish is useful for the micro manipulation, micro injection, biopsy and fertilization in oocyte and embryo manipulation and culturing. The dish allows the user to more readily perform procedures used to fertilize oocytes by Intracytoplasmic sperm injection (ICSI) or other procedures, biopsy embryos and perform additional procedures for use in assisted reproductive techniques (ART), human reproduction and in-vitro fertilization (IVF). The invention allows ease in use, the reduction in the number of micro tools used in the procedure, as opposed to conventional dishes and procedures, and will allow the user to more readily locate the oocytes and embryos to be handled and worked on. The invention will add repetitiveness, consistency, improve efficacy and ease of procedure and may improve outcomes. The invention will also give the user a more ergonomically practical dish for these types of procedures and related protocols. Specimens other than oocytes and embryos can be treated in the container by means of micro manipulation. | 2008-10-09 |
20080248564 | NOVEL PROPERTY EFFECTING AND/OR PROPERTY EXHIBITING COMPOSITIONS FOR THERAPEUTIC AND DIAGNOSTIC USES - The present invention provides an array of compositions useful for effecting and/or exhibiting changes in biological functioning and processing within cells and in biological systems containing such cells. In effect, these compositions combine chemical modifications and/or ligand additions with biological functions. The chemical modifications and/or ligand additions provide additional characteristics to the compositions without interfering substantially with their biological function. Such additional characteristics include nuclease resistance, targeting specific cells or specific cell receptors localizing to specific sites within cells and augmenting interactions between the compositions and target cells of interest as well as decreasing such interactions when desired. Also provided by the present invention are processes and kits. | 2008-10-09 |
20080248565 | ISOLATED PHOSPHOLIPID-PROTEIN PARTICLES - Systems and methods are provided for producing a protein of interest that is typically not amenable to expression in soluble form in in vitro expression systems. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include a scaffold protein such as apolipoprotein or an amphipathic alpha helix containing (“AAHC”) protein, in which higher yields of soluble protein are produced than in the absence of the scaffold protein. The scaffold proteins may be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The scaffold protein may be provided as a protein per se or may be encoded by a nucleic acid template and co-expressed with the protein of interest. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein expression and isolation. | 2008-10-09 |
20080248566 | MATRIPTASE, A SERINE PROTEASE AND ITS APPLICATIONS - The invention is directed to a method of detecting a malignancy or a pre-malignant lesion in breast or other tissue, or a pathologic condition, by detecting the presence of single-chain or two-chain forms of matriptase in the tissue. The invention is further directed to a method of treating malignancies, which have the phenotype of matriptase production by administering a tumor formation inhibiting effective amount of concentrate of Bowman-Birk inhibitor (BBIC), or other matriptase inhibitor. The invention also is directed to nucleic acids encoding a matriptase protein or fragments thereof, and their use for structure elucidation and modeling to identify other inhibitors of matriptase, as well as to methods of identifying matriptase modulating agents, including activators and inhibitors. | 2008-10-09 |
20080248567 | Methods and products for embryonic stem cell culture - The invention relates to methods and media for preparing and maintaining self-renewing pluripotent embryonic stem cells. The methods include, in some embodiments, culturing embryonic stem cells in culture medium that includes culture medium conditioned by cells that express wnt3a. | 2008-10-09 |
20080248568 | Directed Neural Differentiation - Differentiation towards a neural fate, and away from a non-neural fate, is promoted by activation of Notch signalling in ES cells and then transferring the cells into neural differentiation protocols. Media for neural differentiation comprises a Notch activator, e.g. a notch ligand that can be clustered. Genetic manipulation is used as an alternative to media additives for Notch activation. | 2008-10-09 |
20080248569 | SELF-ASSEMBLING PEPTIDE AMPHIPHILES FOR TISSUE ENGINEERING - The present invention provides for compositions and methods for creating self-assembled peptide amphiphile (PA) structures. In particular, the present invention provides for two and three-dimensional structures of crosslinked PA microtexture structures useful for tissue engineering and drug discovery. | 2008-10-09 |
20080248570 | Complexes of hyaluronans, other matrix components, hormones and growth factors for maintenance, expansion and/or differentiation of cells - A method is provided of propagating hepatic cells including hepatic progenitors ex vivo on or in hyaluronans with or without other extracellular matrix components (such as collagens, basal adhesion molecules, proteoglycans or their glycosaminoglycans) and with or without hormones and/or growth factors. Compositions comprising the matrix are also disclosed. Also, the complex can be used for ex vivo tissue engineering or can be used as a scaffold for grafts of cells to be transplanted in vivo. | 2008-10-09 |
20080248571 | Method of Production of Artificial Skin - A method for production of artificial skin by administering matrix metalloproteinase inhibitor or matrix metalloproteinase inhibitor and matrix protein production promoting agent. The matrix metalloproteinase inhibitor is N-hydroxy-2(R)-[[(4-methoxyphenyl)sulfonyl](3-picolyl)amino]-3-methylbutanamide hydrochloride. | 2008-10-09 |
20080248572 | Bioreactor Surfaces - The present invention relates to treatment of polymeric bioreactor surfaces, to promote the proliferation of adherent cells. | 2008-10-09 |
20080248573 | Mixture for Oocytes Maturation in Art Laboratory - Women over 40 years and those who had ovarian problems suffer from low pregnancy rate after Assisted Reproduction Technology (ART) procedure due to immature oocytes. According to the invention a mixture is added to these oocytes. This mixture consists of two substances: Substance 1 which has the ability of stimulating cell proliferation in vitro by increasing the oxygen uptake of the cell and substance 2 which is any of the most used media in ART laboratories. Substance 1 is protein-free haemodialysate of calves blood. The created mixture in more than one concentration succeeded in complete oocytes maturation from immature to complete matured ones, able to be injected and fertilized by sperm. A second aspect of the invention is the use of the mixture to increase motility of sperm by 30% when sperm is incubated before Intrauterine Insemination (IUI). | 2008-10-09 |
20080248574 | ANTAGONISTS OF THE INTERACTION OF MELANOMA INHIBITORY ACTIVITY (MIA) AND P66SHC - The invention provides polypeptides, nucleic acids encoding the polypeptides, optionally in a vector, and their use in methods to disrupt the interaction between p66shc and Melanoma Inhibitory Activity (MIA) in a cell and to sensitize a cell to oxidative stress. | 2008-10-09 |
20080248575 | Drug and Gene Delivery by Polymer Nanonozzle and Nanotip Cell Patch - Delivery of drugs or genes to individual cells is achieved on a nanoscale using electroporation techniques. In one method, a flow-through bioreactor having an inlet and an outlet connected by a flow chamber and a nanoporous membrane positioned in the flow chamber is used. Cells to be electroporated are flowed from the inlet to the outlet, a quantum of molecules of the at least one drug or gene in a fluid medium in the flow chamber. An electrical field applied in the flow chamber provides momentum to the molecules in the nanopores, resulting in delivery of the molecules into the plurality of cells. | 2008-10-09 |
20080248576 | Genetic Inhibition of double-stranded RNA - A process is provided of introducing an RNA into a living cell to inhibit gene expression of a target gene in that cell. The process may be practiced ex vivo or in vivo. The RNA has a region with double-stranded structure. Inhibition is sequence-specific in that the nucleotide sequences of the duplex region of the RNA and of a portion of the target gene are identical. The present invention is distinguished from prior art interference in gene expression by antisense or triple-strand methods. | 2008-10-09 |
20080248577 | A METHOD OF MONITORING A SURFACTANT IN A MICROELECTRONIC PROCESS BY FLUORESCENCE - A method of monitoring a surfactant in a microelectronic process is disclosed. Specifically, the monitoring of a surfactant occurs by studying the fluorescence or electromagnetic emission of a sample collected from a microelectronic process. | 2008-10-09 |
20080248578 | Detection of explosives and other species - The present invention provides a series of systems, devices, and methods relating to the determination of explosives, such as peroxides or peroxide precursors, and other species. Embodiments of the invention may allow a sample suspected of containing an explosive (e.g., a peroxide) or other species to interact with a reactant, wherein the sample may react and cause light emission from the reactant. Advantages of the present invention may include the simplification of devices for determination of peroxide-based explosives, wherein the devices are portable and, in some cases, disposable. Other advantages may include relative ease of fabrication and operation. | 2008-10-09 |
20080248579 | Method of stabilizing human natriuretic peptides - The present invention relates to methods for making stable test samples that can be used in ligand-binding assays for measuring natriuretic peptides. | 2008-10-09 |
20080248580 | Method for Determining the Endogenous Antioxidative Potential of Beverages by Means of Esr Spectroscopy - The present invention relates to a method for determining the endogenous antioxidative potential (EAP) in a beverage sample, preferably beer, wine, juice or mixtures thereof, using ESR spectroscopy. More specifically, the invention relates to a method for measuring a secondary radical, preferably a hydroxyethyl radical, which is stabilized by a spin trap reagent, wherein the spin trap reagent is preferably, N-tert-butyl-α-(4-pyridyl)nitrone N-oxide (POBN). The invention also relates to the use of the method for assessing the oxidative stability of a beverage sample, to the use of a spin trap reagent, preferably POBN, for measuring the EAP, and to a kit for carrying out the method. The invention is characterized in that a method is provided which makes it possible to avoid the pH effect occurring in prior art methods and the associated influence on the generation of radicals in the beverage sample to be measured. | 2008-10-09 |
20080248581 | Method for performing correction of blood glucose assay bias using blood hemoglobin concentration - A device and method for determining a correction factor for correcting the blood glucose assay bias based on sample hematocrit interference in a testing device using the blood hemoglobin concentration. The hemoglobin assay and the glucose assay may be performed using a single combination monitoring device. | 2008-10-09 |
20080248582 | Diagnostic Device and Method - A method of separating a cell-containing sample into a substantially cell-depleted portion, and a cell-containing portion comprising at least one of a stem cell, a lymphocyte, and a leukocyte comprises a step in which the sample is received in a vessel with at least one flexible wall. In another step, an additive and particles are added to the sample, wherein the additive substantially binds to the at least one of the stem cell, lymphocyte, and leukocyte, and the particles and wherein the particles substantially bind to the at least one of the stem cell, lymphocyte, and leukocyte, and the additive, thereby producing a cell-containing network. In a further step, the network is separated from the substantially cell-depleted portion by applying a magnetic force. | 2008-10-09 |
20080248583 | Quantitative and Qualitative Chelation Measuring Methods and Materials - The present invention is directed to a method of assaying for the presence or absence of a metal-ligand chelate in a sample containing either a metal-ligand chelate or a free ligand. The method includes (a) reacting the sample with a luminophore to obtain either a metal-ligand-luminophore chelate and/or a ligand-luminophore adduct; (b) detecting the electromagnetic spectrum of the reaction product; (c) matching the electromagnetic spectrum of the reaction product with an electromagnetic spectrum metal-ligand chelate luminophore chelate or the electromagnetic spectrum of a ligandluminophore adduct; (d) correlating a match of the electromagnetic spectrum of the reaction product and the electromagnetic spectrum of the metalligand-luminophore chelate with the presence of a metal-ligand chelate, or correlating a match of the electromagnetic spectrum of the reaction product and the electromagnetic spectrum of the ligand-luminophore adduct with the absence of a metal-ligand chelate. | 2008-10-09 |
20080248584 | Trityl derivatives for enhancing mass spectrometry - Compounds of formula (IIa): | 2008-10-09 |
20080248585 | Protein/Peptide Sequencing By Chemical Degradation in the Gas Phase - A fast and sensitive method and device for protein sequencing are disclosed. The method uses a combination of Edman degradation chemistry and mass spectrometry to sequence proteins and polypeptides. A peptide degradation reaction is performed on a polypeptide or protein ion reactant in the gas phase. The reaction yields a first ion product corresponding to a first amino acid residue of the polypeptide or protein reactant and a polypeptide or protein fragment ion. The mass-to-charge ratio for the first ion product, or the polypeptide or protein fragment ion, or both, is then determined. The first amino acid residue of the polypeptide or protein reactant is then identified from the mass-to-charge ratio so determined. | 2008-10-09 |
20080248586 | Reaction Vessel, Reaction Vessel Liquid Introducing Device, Liquid Introducing and Reaction Measuring Device, and Liquid Introducing Device - The invention relates to a reaction vessel, a reaction vessel liquid introducing device, a liquid introducing and reaction measuring device, and a liquid introducing device, and is directed to being able to perform temperature control of a liquid stored within the reaction vessel with a high accuracy and faithful responsiveness. The reaction vessel comprises: a storage chamber in which a liquid is storable, that has an opening part; a reaction chamber that is formed thinner or narrower than the storage chamber; and at least one flow passage that communicates between the storage chamber or the exterior and the reaction chamber. The reaction vessel is formed such that it is connectable to a liquid introducing section provided externally, and the liquid can be introduced into the reaction chamber by connecting to the liquid introducing section. | 2008-10-09 |
20080248587 | Methods for Verifying Fluid Movement - Methods for using semiconductor nanocrystals for determining fluid movement, fluid dilution and fluid removal are described. Methods for using semiconductor nanocrystals for monitoring and quantifying the amounts of solid materials dissolved in a liquid are also described. | 2008-10-09 |
20080248588 | SHRIMP ALLERGEN, ANTI-SHRIMP ALLERGEN ANTIBODY, AND USE THEREOF - Provided is a novel shrimp allergen. The shrimp allergen of the present invention includes an isolated shrimp collagen or a polypeptide fragment thereof. According to the present invention, there is provided the novel shrimp allergen, and there are provided, using the allergen, an anti-shrimp allergen antibody, a method of detecting contamination of a shrimp allergen, an agent for detecting contamination of a shrimp allergen, a method of measuring sensitivity to a shrimp allergen, and an agent for measuring sensitivity to a shrimp allergen. | 2008-10-09 |
20080248589 | Sample Presentation Device - The present invention relates to sample presentation devices useful in performing analytical measurements. These devices have been configured to enable various aspects of liquid handling such as: retention, storage, transport, concentration, positioning, and transfer. Additionally, these devices can enhance the detection and characterization of analytes. The sample presentation devices of the present invention are comprised of one or more substrates having a plurality of zones of differing wettability. Methods of analyzing samples using the sample presentation device of the invention, as well as methods of making the sample presentation devices are disclosed. | 2008-10-09 |
20080248590 | Device For Carrying Out A Biological Assay - An integrated lab-on-a-chip device for carrying out an assay to detect the presence of a biological molecule in a fluid sample, the device comprising: (a) an inlet for a fluid sample; (b) one or more reaction sites each in fluid communication with the inlet; (c) one or more reagent reservoir systems each containing reagents required for an assay to detect a biological molecule, the reagents being arranged sequentially in each reservoir system in the order in which they are required for the assay and separated from one another by a fluid. | 2008-10-09 |
20080248591 | Gas bubble biosensor - The present invention describes a biosening device and method. Specifically, binding of target analyte perturbs the surface of a sensor strip so that gas bubbles are generated in solution. Said gas bubbles may be detected for determination of analyte presence in sample. | 2008-10-09 |
20080248592 | Detection of target analytes using particles and electrodes - The invention relates to the use of particles comprising binding ligands and electron transfer moieties (ETMs). Upon binding of a target analyte, a particle and a reporter composition are associated and transported to an electrode surface. The ETMs are then detected, allowing the presence or absence of the target analyte to be determined. | 2008-10-09 |
20080248593 | METHOD FOR DETECTION OF FLUORIDE OR HYDROGEN FLUORIDE AND DETECTION KIT - The present invention relates to a method for detecting and/or measuring the concentration of fluoride (F | 2008-10-09 |
20080248594 | ORGANIC NON-SUGAR COMPOUNDS FOR PROTECTION OF BIOLOGICALLY ACTIVE MOLECULES AND CONJUGATE LABELS AND METHODS OF USE THEREOF - Provided are methods comprising the use of non-sugar organic compatible solutes for protection and preservation of the activity of biologically active molecules and conjugate labels. The methods are particularly adaptable for use in conjunction with immunoassays, such as for example, immunochromatographic test assays and may be incorporated into any test methodology wherein a dry test strip is used as a carrier for depositing, mobilizeable and/or immobilized biologically active molecules and/or conjugate labels. | 2008-10-09 |
20080248595 | Method for Manufacturing Semiconductor Device and Computer Storage Medium - A method for fabricating a semiconductor device includes the steps of forming a PbTiOx film having a predominantly (111) orientation on a lower electrode as a nucleation layer by an MOCVD process with a film thickness exceeding 2 nm, and forming a PZT film having a predominantly (111) orientation on the nucleation layer, wherein the step of forming the PbTiOx film is conducted under an oxygen partial pressure of less than 340 Pa. | 2008-10-09 |
20080248596 | Method of making a circuitized substrate having at least one capacitor therein - A method of making a circuitized substrate which includes at least one and possibly several capacitors as part thereof. In one embodiment, the substrate is produced by forming a layer of capacitive dielectric material on a dielectric layer and thereafter forming channels with the capacitive material, e.g., using a laser. The channels are then filled with conductive material, e.g., copper, using selected deposition techniques, e.g., sputtering, electro-less plating and electroplating. A second dielectric layer is then formed atop the capacitor and a capacitor “core” results. This “core” may then be combined with other dielectric and conductive layers to form a larger, multilayered PCB or chip carrier. In an alternative approach, the capacitive dielectric material may be photo-imageable, with the channels being formed using conventional exposure and development processing known in the art. In still another embodiment, at least two spaced-apart conductors may be formed within a metal layer deposited on a dielectric layer, these conductors defining a channel there-between. The capacitive dielectric material may then be deposited (e.g., using lamination) within the channels. | 2008-10-09 |
20080248597 | Methods for determining a dose of an impurity implanted in a semiconductor substrate and an apparatus for same - Methods of determining a total impurity dose for a plasma doping process, and an apparatus configured to determine same. A total ion dose implanted in a semiconductor substrate is directly measured, such as by utilizing a Faraday cup. A ratio of impurity-based ion species to non-impurity-based ion species in a plasma generated by the plasma doping process and a ratio of each impurity-based ion species to a total impurity-based ion species in the plasma are directly measured. The ratios may be directly measured by ion mass spectroscopy. The total ion dose and the ratios are used to determine the total impurity dose. The apparatus includes an ion detector, an ion mass spectrometer, a dosimeter, and software. | 2008-10-09 |
20080248598 | METHOD AND APPARATUS FOR DETERMINING CHARACTERISTICS OF A STRESSED MATERIAL USING SCATTEROMETRY - A method includes illuminating at least a portion of a first grid including a first plurality of stressed material regions formed at least partially in a semiconducting material. Light reflected from the illuminated portion of the first grid is measured to generate a first reflection profile. A characteristic of the first plurality of stressed material regions is determined based on the first reflection profile. A test structure includes a first plurality of stressed material regions recessed with respect to a surface of a semiconductor layer and defining a first grid. A first plurality of exposed portions of the semiconductor layer is disposed between each of the first plurality of stressed material regions. | 2008-10-09 |
20080248599 | Rapid Thermal Anneal Equipment and Method Using Sichrome Film - A method of determining the degree of calibration of an RTP chamber ( | 2008-10-09 |
20080248600 | METHOD AND DEVICE FOR WAFER BACKSIDE ALIGNMENT OVERLAY ACCURACY - A method for wafer backside alignment overlay accuracy includes forming a buried layer on a front-side of a wafer; forming a conductive layer on the buried layer and patterning a first test structure and a second test structure therein; forming an etch stop layer on the conductive layer; etching through the wafer from the backside to perform an alignment process with the first test structure; and determining an overlay accuracy of the alignment process with the second test structure. The first test structure includes an optical vernier and the second test structure includes an electrical testing structure. | 2008-10-09 |
20080248601 | Method of fusing trimming for semiconductor device - Deviation occurring in a particular region in a plane of a resistor group which constitutes a semiconductor integrated circuit is improved and a quick increase in yield is accomplished. Provided is a fuse trimming method for a semiconductor device in which circuit elements such as transistors and resistors are formed on a semiconductor wafer and which has fuse elements capable of adjusting a resistance value of the resistors by laser trimming, including a resistor correction step of correcting in the particular region of the semiconductor wafer the resistance value of the resistors based on an amount of deviation from a target value of the resistance value of the resistors. | 2008-10-09 |