40th week of 2011 patent applcation highlights part 52 |
Patent application number | Title | Published |
20110244449 | METHODS OF DETERMINING COPY NUMBER OF A GENETIC LOCUS - Disclosed are methods of determining in a sample, the molar ratio of a query locus to a reference locus, comprising: 1) forming one or more mixtures, each mixture comprising: a) a reference-query coupled probe comprising a nucleobase polymer comprising i) a probe reference locus comprising a probe reference allele, and ii) a probe query locus comprising a probe query allele, and b) a sample comprising i) a sample reference locus comprising a sample reference allele and ii) 0 or greater copies of a sample query locus comprising a sample query allele; 2) for each mixture, determining a) the amount of probe reference allele as a fraction of total reference allele and b) the amount of probe query allele as a fraction of total query allele; and 3) calculating the molar ratio of the sample query allele to the sample reference allele. | 2011-10-06 |
20110244450 | PRIMATE TOTIPOTENT AND PLURIPOTENT STEM CELLS PRODUCED BY SOMATIC CELL NUCLEAR TRANSFER - Purified totipotent stem cells and pluripotent stems cells derived by somatic cell nuclear transfer are disclosed herein, as well as cell lines, multipotent cells and differentiated cells produced from these stem cells. The stem cells are produced from an enucleated host cell from a first donor and nuclear genetic material from a somatic cell of a second donor. Methods for making and using such compositions of such stem cells are also provided. | 2011-10-06 |
20110244451 | Methods for prenatal diagnosis of chromosomal abnormalities - Chromosomal abnormalities are responsible for a significant number of birth defects, including mental retardation. The present invention is related to methods for non-invasive and rapid, prenatal diagnosis of chromosomal abnormalities based on analysis of a maternal blood sample. The invention exploits the differences in DNA between the mother and fetus, for instance differences in their methylation states, as a means to enrich for fetal DNA in maternal plasma sample. The methods described herein can be used to detect chromosomal DNA deletions and duplications. In a preferred embodiment, the methods are used to diagnose chromosomal aneuploidy and related disorders, such as Down's and Turner's Syndrome. | 2011-10-06 |
20110244452 | SEQUENCE DATA BY REDUCTION OF NOISE DUE TO CARRY-OVER PRIMER - The present invention provides methods of reducing the background signal of a nucleic acid sequencing reaction. In particular, the invention provides methods of specifically degrading unwanted chain termination reaction products generated by the extension of primers carried over from the amplification step of the sequencing reaction. These methods are amenable for use with both one step and two-step amplification/chain termination reaction sequencing protocols. | 2011-10-06 |
20110244453 | SALMONELLA DETECTION ASSAY - There is provided a method and reagents for detecting | 2011-10-06 |
20110244454 | FREEZE-DRIED COMPOSITIONS FOR BIOCHEMICAL REACTIONS - The use of raffinose as a glass-forming agent for freeze-dried compositions intended for use in conducting chemical or biochemical reactions such as the PCR. Thus, for instance, there is provided a composition for carrying out a chemical or biochemical reaction, said composition being in a freeze-dried form and comprising (i) a set of reagents comprising at least some of the chemical or biochemical reagents necessary for conducting said chemical or biochemical reaction, and (ii) raffinose. Kits comprising these compositions and methods of using them form a further aspect of the invention. | 2011-10-06 |
20110244455 | DIGITAL ANALYTE ANALYSIS - The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons. | 2011-10-06 |
20110244456 | HERBICIDE TOLERANT COTTON PLANTS AND METHODS FOR IDENTIFYING SAME - The invention provides specific transgenic cotton plants, plant material and seeds, characterized in that these products harbor a specific transformation event at a specific location in the cotton genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples. | 2011-10-06 |
20110244457 | IMMUNO-AMPLIFICATION - A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte. | 2011-10-06 |
20110244458 | Methods and Nucleic Acids for Analyses of Cellular Proliferative Disorders - Aspects of the invention provide methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. Particular aspects disclose and provide genomic sequences the methylation patterns of which have substantial utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients. | 2011-10-06 |
20110244459 | METHODS FOR IDENTIFYING ERBB2 ALTERATION IN TUMORS - Methods for identifying ERBB2 (also named HER2) alteration in tumors, in particular cancer, based on the analysis of the expression of at least three genes of the ERBB2 amplicon located within less than one megabase on either side of ERBB2, and eventually of the gene corresponding to the Affymetrix probeset 234046_at (SEQ ID NO: 31), as well as a poynucleotide library useful for the molecular characterization of a cancer including polynucleotide sequences for detecting the genes, and a kit including the library. | 2011-10-06 |
20110244460 | METHOD FOR DETECTING CONTROLS FOR NUCLEIC ACID AMPLIFICATION AND USE THEREOF - The present invention provides a control detection method for easily detecting a positive control and a negative control simultaneously in one reaction system. An amplification reaction is carried out by adding a control template nucleic acid to a reaction system for detecting controls. The template nucleic acid can be amplified by a primer capable of amplifying an objective target sequence. An amplification region of the control template nucleic acid amplified by the primer can be hybridized with a detection probe capable of hybridizing to the target sequence. A Tm value (Tm | 2011-10-06 |
20110244461 | METHOD FOR PREPARING STOOL SAMPLE, SOLUTION FOR PREPARING STOOL SAMPLE AND STOOL COLLECTION KIT - The present invention relates to the providing of a method for preparing a stool sample that enables a nucleic acid in a stool to be stably preserved without requiring a complex procedure, a solution for preparing a stool sample, a stool collection kit used in that method, and a method for recovering and analyzing a nucleic acid in a stool using a stool sample prepared using the preparation method of the present invention. A method for preparing a stool sample according to the present invention is a method for preparing a stool sample being used for analyzing a nucleic acid contained in the stool, and is characterized in that a collected stool is mixed with a solution having a protease inhibitor as an active ingredient. | 2011-10-06 |
20110244462 | Method for Estimating Telomere Length - Knowledge about telomere length is highly relevant in cancer and age related research. Currently applied methods for determining telomere length are subject to several drawbacks preventing fast and reliable information concerning telomere length. The present invention relates to a method for determining telomere length which is fast and reliable. The method is PCR based and may advantageously be performed in a “one tube system”, whereby time consuming and inconvenient handling steps are avoided. The method comprises annealing of up- and downstream tags to telomere fragments and subsequent PCR amplification of telomere fragments using primers having a sequence complementary or identical to at least part of the up- and downstream oligonucleotide tags. | 2011-10-06 |
20110244463 | METHOD FOR COLUMBIDAE GENDER IDENTIFICATION, NUCLEOTIDE SEQUENCE FOR COLUMBIDAE GENDER AND NUCLEOTIDE PRIMER PAIR FOR COLUMBIDAE GENDER - The invention provides a method for Columbidae gender identification including: providing a DNA sample of a bird belonging to the Columbidae family; performing a polymerase chain reaction to the DNA sample with a first primer pair of a first primer designed within the region of the SEQ ID. NO.: 9 or a complementary sequence thereof and a P2 primer (SEQ ID. NO: 1) or a complementary sequence thereof, and a second primer pair of a second primer designed within the region of the SEQ ID NO.: 10 or a complementary sequence thereof and a P2 primer (SEQ ID. NO: 1) or a complementary sequence thereof; and determining gender by performing a melting curve analysis to a product from the polymerase chain reaction, wherein the result showing two peaks indicate a female gender and showing one peak indicate a male gender. | 2011-10-06 |
20110244464 | IDENTIFICATION OF GENES OR POLYPEPTIDES THE EXPRESSION OF WHICH CORRELATES TO FERTILITY, OVARIAN FUNCTION AND/OR FETAL/NEWBORN VIABILITY - A genetic means of determining whether a female subject produces “pregnancy competent” oocytes is provided. The means comprises detecting the level of expression of one or more genes that are expressed at characteristic levels (upregulated or downregulated) in cumulus cells derived from pregnancy competent oocytes. This characteristic gene expression level, or pattern referred to herein as the “pregnancy signature”, also can be used to identify subjects with underlying conditions that impair or prevent the development of a viable pregnancy, e.g., pre-menopausal condition, other hormonal dysfunction, ovarian dysfunction, ovarian cyst, cancer or other cell proliferation disorder, autoimmune disease and the like. Microarrays containing “pregnancy signature” genes or corresponding polypeptides provide another preferred aspect of the invention. Still further, the subject invention can be used to derive animal models, e.g., non-human primate animal models, for the evaluation of the efficacy of putative female fertility treatments. | 2011-10-06 |
20110244465 | METHODS OF PREDICTING AND MONITORING TYROSINE KINASE INHIBITOR THERAPY - The present invention provides methods for analyzing a combination of biomarkers to individualize tyrosine kinase inhibitor therapy in patients who have been diagnosed with cancer. In particular, the assay methods of the present invention are useful for predicting, identifying, or monitoring the response of a tumor, tumor cell, or patient to treatment with a tyrosine kinase inhibitor using an algorithm based upon biomarker profiling. The assay methods of the present invention are also useful for predicting whether a patient has a risk of developing toxicity or resistance to treatment with a tyrosine kinase inhibitor. In addition, the assay methods of the present invention are useful for monitoring tyrosine kinase inhibitor therapy in a patient receiving the drug to evaluate whether the patient will develop resistance to the drug. Furthermore, the assay methods of the present invention are useful for optimizing the dose of a tyrosine kinase inhibitor in a patient receiving the drug to achieve therapeutic efficacy and/or reduce toxic side-effects. | 2011-10-06 |
20110244466 | NUCLEIC ACID TESTING DEVICE AND METHOD - Devices and systems for extracting, purifying and amplifying nucleic acids, and methods for use of such devices and systems. The devices have top sections which include a plurality of syringe vessels with applicable reagent materials, as well as a channel for a specimen collection dropper. Plungers force the materials sequentially into reaction chambers in the middle sections. Once the samples are extracted and purified, they are drawn by a vacuum into PCR devices where they are subjected to denaturing, annealing and extension steps and amplified. Interrogation of flow cells provide real-time quantitative detection. | 2011-10-06 |
20110244467 | MICROFLUIDIC APPARATUS AND METHOD FOR DNA EXTRACTION, AMPLIFICATION AND ANALYSIS - An integrated gel based microfluidic sample processing device, suitable for forensic investigations at the scene of a crime, including a substrate having a plurality of micro-channels to form at least a DNA extraction chamber in fluidic cooperation with an amplification chamber which in turn is in fluidic cooperation with a separation and detection channel. The micro-channels containing a DNA extraction material and gel based reaction reagents necessary for processing the sample. The device further having electrical contacts for coupling to an external power source and capable of inducing electro-kinetic manipulation of the gel based reagents and DNA extracted from the sample throughout the device. | 2011-10-06 |
20110244468 | PARALLEL EXTRACTION OF DIFFERENT BIOMOLECULES FROM FORMALIN-FIXED TISSUE - The present invention relates to a method of isolating/extracting in parallel various biomolecules, in particular nucleic acids and proteins, from the same fixed biological samples, to the quantification and analysis of the biomolecules isolated by the method of the invention, and to a kit for isolating/extracting in parallel various biomolecules from a fixed sample, to the use of said kit for diagnosing, prognosing, deciding the therapy of and monitoring the therapy of a disease. | 2011-10-06 |
20110244469 | METHOD FOR QUANTIFYING INITIAL CONCENTRATION OF NUCLEIC ACID FROM REAL-TIME NUCLEIC ACID AMPLIFICATION DATA - Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration. | 2011-10-06 |
20110244470 | ISOLATED TRANSGENIC MAMMALIAN NEURAL CELL FOR DETECTION OF A SAMPLE CONTAINING A CHEMICAL SUBSTANCE DAMAGE TO NEUROLOGICAL SYSTEM OR SELECTION OF DRUGS FOR TREATING NEURODEGENERATIVE DISORDERS - The invention is to provide an isolated transgenic mammalian neural cell, which comprises at least one heterologous vector expressing AhR/ARNT. Also provided is methods the detection of a sample containing a chemical substance damage to the nervous system and the selection of drugs for treating neurodegenerative disorders. | 2011-10-06 |
20110244471 | SCREENING METHOD AND VECTOR, VECTOR LIBRARY, AND ASSAY KIT USED THEREFOR - According to one embodiment, a method of screening an enhancer and/or a promoter, includes culturing a host cell into which an amplifiable vector is introduced, extracting the vector from the host cell and obtaining the DNA fragment from the extracted vector, wherein the vector includes a DNA fragment to be determined, a gene that is functionally linked downstream of the DNA fragment and encodes a protein to initiate self-replication, and a gene that encodes a replication origin sequence. | 2011-10-06 |
20110244472 | Cancer stem cell immortalization - The present invention relates to the preparation and use of immortalized cancer stem cells. The immortalized cancer stem cells of the invention may be used in assays to identify anti-cancer compounds as well as molecules critical to carcinogenesis. Further, cancer stem cells may be harvested from a patient and used, according to the invention, to select agents more likely to be effective in treating the cancer of that particular patient. | 2011-10-06 |
20110244473 | Methods and materials for observing apoptosis - The invention provides methods and materials for observing protein fragments generated during apoptosis in order to observe this process in mammalian cells. Embodiments of the invention can be used for example to observe apoptosis in order to examine the sensitivity of a mammalian cancer cell to apoptosis inducing agents. | 2011-10-06 |
20110244474 | Rapid screening of biologically active nucleases and isolation of nuclease-modified cells - Disclosed herein are methods and compositions for rapidly identifying active nucleases and cells having nuclease-mediated genomic modifications. | 2011-10-06 |
20110244475 | MN Gene and Protein - Identified herein is the location of the MN protein binding site, and MN proteins/polypeptides that compete for attachment to vertebrate cells with immobilized MN protein. Such MN proteins/polypeptides prevent cell-cell adhesion and the formation of intercellular contacts. The MN protein binding site is a therapeutic target that can be blocked by organic or inorganic molecules, preferably organic molecules, more preferably proteins/polypeptides that specifically bind to that site. Therapeutic methods for inhibiting the growth of preneoplastic/neoplastic vertebrate cells that abnormally express MN protein are disclosed. Vectors are provided that encode the variable domains of MN-specific antibodies and a flexible linker polypeptide separating those domains. Further vectors are disclosed that encode a cytotoxic protein/polypeptide operatively linked to the MN gene promoter, and which vectors preferably further encode a cytokine. The MN gene promoter is characterized, and the binding site for a repressor of MN transcription is disclosed. | 2011-10-06 |
20110244476 | METHOD FOR EVALUATION OF QUALITY OF BLOOD SAMPLE - A method for evaluating the quality of a blood sample, comprising the steps of: mixing labeled anti-cortisol antibodies with a blood sample to be subjected, to conduct the antigen-antibody reaction of the labeled anti-cortisol antibody with cortisol contained in the blood sample, developing a mixture obtained in the above step on an immunochromatographic test strip having a substrate on which cortisol is immobilized to cause the antigen-antibody reaction of the labeled anti-cortisol antibodies which are free in the mixture with the cortisol immobilized on the substrate, thereby bonding the antibody to the cortisol, determining the amount of the labeled anti-cortisol antibodies bonded to the cortisol in the above step, and evaluating whether or not the blood sample has a quality suitable for suprarenal vein sampling test on the basis of the amount of the labeled anti-cortisol antibodies determined in the above step. | 2011-10-06 |
20110244477 | ANTIBODY-SECRETING CELL ASSAY - An improved assay is described where a surface is provided with immobilized anti-Ig antibodies rather than antigen and where specific antibody-secreting cells (ASC) are detected using soluble antigen probes containing one of several possible labels. The method gives improved sensitivity with less background and is also more representative because antigen binding does not employ immobilized antigen. The assay is particularly effective for measuring antibody secreting cells against HIV, for determining whether an infection is acute as opposed to old or latent, for mapping epitopes and for measuring for ASCs against different antigens in the same reaction. | 2011-10-06 |
20110244478 | Methods and Compositions for Treating Bleeding Disorders - Aspects of the invention include methods for enhancing blood coagulation in a subject. In practicing methods according to certain embodiments, an amount of a non-anticoagulant sulfated polysaccharide (NASP) is administered to a subject to enhance blood coagulation in the subject. Also provided are methods for preparing a NASP composition having blood coagulation enhancing activity. Compositions and kits for practicing methods of the invention are also described. | 2011-10-06 |
20110244479 | Plasmon Resonant Particles, Methods and Apparatus - A method and apparatus for interrogating a target having a plurality of plasmon resonant particles (PREs) distributed in the target are disclosed. In the method, a field containing the target is illuminated, and one or more spectral emission characteristics of the light-scattering particles in the field are detected. From this data, an image of positions and spectral characteristic values in the field is constructed, allowing PREs with a selected spectral signature to be discriminated from other light-scattering entities, to provide information about the field. Also disclosed are a novel PRE composition for use in practicing the method, and a variety of diagnostic applications of the method. | 2011-10-06 |
20110244480 | METHOD OF DIAGNOSIS AND KIT THEREFOR - The invention provides kits and methods for detecting or monitoring the number of cells in sample. The cell comprises a cell surface associated protein (CSAP) comprising a cytoplasmic (cytosolic) and an extracellular (ecto) domain. The kit comprises: (i) a chromatographic device; and (ii) a CSAP-binding agent. The method comprises: (i) optionally contacting the sample with an agent capable of lysing or permeabilizing CSAP bearing cells; (ii) contacting the sample with a CSAP-binding agent that binds to the cytoplasmic domain of the CSAP; and (iii) directly or indirectly evaluating the level or presence of bound CSAP in the sample. | 2011-10-06 |
20110244481 | COELENTERAZINE ANALOGUES AND COELENTERAMIDE ANALOGUES - Coelenterazine analogues with different luminescence properties from conventional ones and coelenteramide analogues with different fluorescence properties from conventional ones have been desired. The invention provides coelenterazine analogues modified at the 8-position of coelenterazine and coelenteramide analogues modified at the 2- or 3-position of coelenteramide. | 2011-10-06 |
20110244482 | ASSESSMENT OF SUBCHONDRAL BONE REMODELLING BY MEASURING CATHEPSIN K FRAGMENTS OF COLLAGEN TYPE II - A method of assay to determine the extent of collagen type II resorption activity comprising measuring the level of fragments of collagen type II that contain a cathepsin K generated neo-epitope not shared by collagen type I by binding the neo-epitope with an antibody specific for the neo-epitope and detecting the level of binding of said binding partner. | 2011-10-06 |
20110244483 | ASSESSMENT OF PROTEIN DEGRADATION BY MEASUREMENT OF COLLAGEN FRAGMENTS - A method of assay measuring in a biological sample fragments of a protein that contain an N-terminal neo-epitope and a C-terminal neo-epitope, each generated by protease cleavage of said protein, comprises binding the N-terminal neo-epitope with a first specific antibody and binding the C-terminal neo-epitope with a second specific antibody, and detecting the extent of dual binding of said antibodies. | 2011-10-06 |
20110244484 | METHOD OF DIAGNOSING ALZHEIMER'S DISEASE USING GIANT MAGNETORESISTANCE SENSOR AND MAGNETIC BEAD-POLYPROTEIN COMPLEX FOR DIAGNOSING ALZHEIMER'S DISEASE - Provided are a method of diagnosing Alzheimer's disease using a giant magnetoresistance sensor and a magnetic bead-polyprotein complex for diagnosing Alzheimer's disease. The method of diagnosing Alzheimer's disease using the giant magnetoresistance sensor may be applied to diagnose Alzheimer's disease more easily and simply using the giant magnetoresistance sensor than using conventional fluorescent materials or genetic analyses, and the magnetic bead-polyprotein complex may be mass-produced as a diagnostic biosensor for Alzheimer's disease, and thus to be useful to monitor and treat Alzheimer's disease. | 2011-10-06 |
20110244485 | ANTI-TUNA VASA ANTIBODY - An object of the present invention is to provide a means for distinguishing between a germ cell derived from a donor (tuna) and a germ cell derived from a recipient in a method for inducing differentiation of a tuna germ cell, wherein a primordial germ cell derived from the tuna is transplanted into an early embryo of the heterologous recipient fish. The present inventors compared a Vasa amino acid sequence of bluefin tuna with those of other fish (black skipjack, skipjack, chub mackerel, blue mackerel, round frigate mackerel and frigate mackerel), identified amino acid sequence regions specific to bluefin tuna, and, by using the amino acid sequences specific to bluefin tuna as antigens, successfully produced monoclonal antibodies specifically recognizing primordial germ cells, spermatogonia, oogonia or oocytes derived from bluefin tuna, thus accomplishing the present invention. | 2011-10-06 |
20110244486 | METHODS AND COMPOSITIONS FOR THE DIAGNOSIS AND TREATMENT OF DIABETES - Methods, assays and compositions for the diagnosis and treatment of diabetes, in which the glutamate transporters and/or receptors expressed in pancreatic islet cells are used as therapeutic targets or tools for the identification or treatment of individuals suffering from or susceptible to diabetes. | 2011-10-06 |
20110244487 | METHODS FOR TESTING BINDING OF A LIGAND TO A G PROTEIN-COUPLED RECEPTOR - The present invention relates to methods for testing for the binding of a ligand to a G Protein-Coupled Receptor. In particular, the methods of the invention are useful in high throughput screening for ligands which bind to G Protein-Coupled Receptors. | 2011-10-06 |
20110244488 | CHITIN-INDUCED IMMUNE RESPONSE BASED METHOD FOR DIAGNOSING ALLERGIC ASTHMA IN PATIENTS - A method of diagnosing allergic asthma in patients, including the steps of: obtaining from a subject a first sample of peripheral blood immune cells and a second sample of peripheral blood immune cells; adding chitin to the first sample; measuring the average amount of IFN-γ in both the first sample and the second sample to get a first value and a second value respectively; and dividing the first value with the second value to get a ratio, wherein if the ratio is smaller than a threshold value, the subject is diagnosed as an allergic asthma patient. | 2011-10-06 |
20110244489 | METHOD FOR THE EARLY DETECTION OF RENAL INJURY - A method and kit for detecting the immediate or early onset of renal disease and injury, including renal tubular cell injury, utilizing NGAL as an immediate or early on-set biomarker in a sample of blood serum. NGAL is a small secreted polypeptide that is protease resistant and consequently readily detected in the blood serum following renal tubule cell injury. NGAL protein expression is detected predominantly in proximal tubule cells, in a punctuate cytoplasmic distribution reminiscent of a secreted protein. The appearance NGAL in the serum is related to the dose and duration of renal ischemia and nephrotoxemia, and is diagnostic of renal tubule cell injury and renal failure. NGAL detection is also a useful marker for monitoring the nephrotoxic side effects of drugs or other therapeutic agents. | 2011-10-06 |
20110244490 | DETECTION AND MONITORING OF LIVER DAMAGE - A method of detecting liver damage in a subject comprises measuring the level of caspase-3 generated cytokeratin-18 fragments in the bodily sample. The level of measuring the level of caspase-3 generated cytokeratin-18 fragments is then correlated with liver disease progression. | 2011-10-06 |
20110244491 | PEPTIDES DERIVED FROM HUMAN BPLP PROTEIN, POLYNUCLEOTIDES CODING FOR SAID PEPTIDES AND ANTIBODIES DIRECTED AGAINST SAID PEPTIDES - The invention relates to an in vitro method for prognosis, diagnosis or determination of the evolution of a condition involving an altered production of Basic Proline-rich Lacrimal Protein (BPLP) or of any of its maturation products, by detecting, or quantifying in a biological sample of a test subject, a BPLP protein or a maturation product thereof, and comparing the production of BPLP protein or maturation product with the production of the same in a biological sample of a control subject. | 2011-10-06 |
20110244492 | IMMUNOASSAYS FOR CITRULLINATED PROTEINS - Methods and kits are provided for assessing radiation injury and exposure in a mammal. The methods comprise the steps of: obtaining one or more test samples from the mammal, contacting the test samples with an antibody immunoreactive with a citrullinated protein to form an immunocomplex; and detecting the immunocomplex with an ELISA; wherein a decrease in the quantity of the immunocomplex in the test samples, as compared to the quantity of immunocomplexes formed under identical conditions with the same antibody and a control sample from one or more mammals known to have a lower degree of radiation injury or exposure, indicates a higher degree of radiation injury and exposure to the mammal. The information obtained from such methods can be used by a clinician to accurately assess the extent of radiation injury/exposure in the mammal, and thus will provide a valuable tool for determining treatment protocols on a subject by subject basis. | 2011-10-06 |
20110244493 | CELL-BASED DETECTION OF APF THROUGH ITS INTERACTION WITH CKAP4 FOR DIAGNOSIS OF INTERSTITIAL CYSTITIS - An assay system designed to detect a protein biomarker in urine that is diagnostic for interstitial cystitis (IC). The presence of a 9 amino acid glycopeptide, antiproliferative factor (APF), in urine is unique to patients with IC. Urine samples from patients who exhibit symptoms consistent with IC are added to the assay system. Binding of APF to the cytoskeletal associated protein 4 (CKAP4) is positive for the presence of APF in urine and diagnostic for IC. The diagnostic system is a significant and surprising advance in diagnosis of IC and has commercial applications relevant to women and men who suffer from symptoms consistent with IC. | 2011-10-06 |
20110244494 | REAGENTS, METHODS AND KITS FOR THE UNIVERSAL RAPID IMMUNO-DETECTION - Novel immuno-detection methods, kits and reagents are provided. The Combination of this invention, combining at least two of the following reagents of a Non-specific Competitor, a Specific Indicator, a primary antibody and an antigen, provides a faster and easier method for an immuno-detection, combining at least two of the following steps of blocking, antigen binding, primary antibody binding and 2 | 2011-10-06 |
20110244495 | METHOD FOR TESTING ALZHEIMER'S DISEASE BY MEASURING DEGRADATION RATE OF -AMYLOID IN BLOOD AND DIAGNOSTIC REAGENT - Provided is a method of testing Alzheimer's disease using serum or plasma as a sample. It is found that a β-amyloid peptide added to a blood sample is degraded. The degradation activity thereof was compared between the blood samples of normal subjects and Alzheimer's disease patients, and it is also found that the degradation activity is significantly higher in the blood of the normal subjects. | 2011-10-06 |
20110244496 | HIV REVERSE TRANSCRIPTASE COMPOSITIONS AND METHODS - The present invention provides engineered novel variants of human immunodeficiency virus reverse transcriptase (HIV-RT) capable of being expressed in large quantity and that with polymerase and RNase H activity in a form that facilitates crystallization and high resolution structure resolution following X-ray diffraction. The present invention facilitates high resolution determination of RT in complexes with RT drugs and RT inhibitors, and provides methods for systematic generation of variants and for structure based identification and design of novel RT inhibitors. | 2011-10-06 |
20110244497 | METHOD FOR JUDGING SUSCEPTIBILITY OF CANCER CELLS TO ANTHRACYCLINE ANTICANCER AGENT AND COMPUTER PROGRAM - The present invention provides a method for judging susceptibility of cancer cells contained in a biological sample to an anthracycline anticancer agent comprising steps of: measuring expression levels of GST-π of cancer cells contained in a biological sample; and judging the susceptibility of cancer cells contained in a biological sample to an anthracycline anticancer agent as high when the expression level of GST-π obtained by the measuring process is high, and a computer program which makes a computer execute the method. | 2011-10-06 |
20110244498 | TYROSINE KINASE-INDUCIBLE DOMAINS - The present invention relates to a tyrosine kinase-inducible domain (pKID) and uses thereof. An isolated polypeptide comprising the pKID, and an isolated polynucleotide comprising a nucleic acid sequence encoding the pKID are provided. Also provided are methods for determining tyrosine kinase and/or phosphatase activity in a sample and for identifying an agent that inhibits a tyrosine kinase or phosphatase using a polypeptide comprising the pKID. | 2011-10-06 |
20110244499 | METHODS OF ENZYMATIC HYDROLYSIS - In one embodiment the instant invention generally pertains to a method for producing glucose for fermentation. The method comprises first treating a biomass with acid and heat under conditions sufficient to produce a composition mixture comprising cellulose suitable for enzymatic hydrolysis. Next, at least a portion of the cellulose of step (a) is enzymatically hydrolyzed under conditions sufficient to form a composition comprising glucose. The glucose is then fermented. Advantageously, one or more reaction conditions are more efficient because they are selected by first measuring an initial hydrolysis rate of said biomass and then selecting one or more appropriate reaction conditions based upon said initial hydrolysis rate. | 2011-10-06 |
20110244500 | Gel electrophoresis method useful for resolution and characterization of nerve tissue ultra high molecular weight protein aggregates - The instant disclosure describes an electrophoretic procedure capable of resolving and isolating ultra high molecular weight (MW) protein aggregates from nerve tissue. The procedure is based on the use of composite agarose-polyacrylamide gel electrophoresis (CAPAGE) and resolves proteins and protein aggregates over the range of from approximately 225 kDa to approximately 30,000 kDa. Triton X-100 precipitation is used to obtain a cytoskeleton protein fraction that is subsequently resuspended and subjected to gel electrophoresis. This method demonstrates that a protein aggregate of approximately 30,000 kDa is characteristic of normal murine spinal cord tissue and that the amount of said protein aggregate is increased in spinal cord homogenate obtained from transgenic mice bearing copies of a mutant human gene characteristic of familial amyotrophic lateral sclerosis. This method for separating nerve tissue ultra high MW cytoskeleton protein aggregates can prove useful in a variety of future biophysical and pharmacological studies related to the etiologies of Charcot-Marie-Tooth disease, Alzheimer's disease, Parkinson's disease, diseases based on expansions in tandem DNA repeats, spinal muscular atrophy, Friedreich's ataxia, giant axon neuropathy, juvenile ceroid-lipofuscinosis, amyotrophic lateral sclerosis, diabetic polyneuropathy and Down's syndrome. | 2011-10-06 |
20110244501 | CANCER STEM CELLS - Cancer stem cell populations characterized by expression of CD44 | 2011-10-06 |
20110244502 | HORMONE RESPONSIVE TISSUE CULTURE SYSTEM AND USES THEREOF - The invention provides tissue culture system for primary cells (e.g. normal mammalian primary epithelial progenitors). This system includes: a) a serum-free, chemically defined cell culture media; and, b) methods for isolation and in vitro long-term propagation of primary cells (e.g. primary epithelial cells). Primary cells so isolated and cultured can be kept undifferentiated and proliferate for many weeks (>15 weeks) or population doubling (>35 PD) without senescence, or any detectable genetic alterations. Upon changing media/culture conditions, these cells can be induced to differentiate. | 2011-10-06 |
20110244503 | System and Method for Anti-Cancer Drug Candidate Evaluation - The disclosure provides a method of evaluating the ability of an anti-cancer drug candidate to induce apoptosis in a known cancer cell line by placing a single-cell suspension of a known cancer cell line in a well of a plate, adding at least one drug candidate to the well in an amount sufficient to achieve a target drug candidate concentration, measuring the optical density at selected time intervals for a selected duration of time, determining a kinetic units value from the optical density and time measurements, and correlating the kinetic units value with an ability of the anti-cancer drug candidate to induce apoptosis in the cancer cell line if the kinetic units value is positive. A similar method may be used to evaluate the ability of an anti-cancer drug candidate to induce apoptosis in a cancer type. | 2011-10-06 |
20110244504 | BIOMARKERS OF INFLAMMATION IN BRUCH'S MEMBRANE OF THE HUMAN RETINA - A method of diagnosing a patient with age-related macular degeneration (AMD), by detecting biomarkers in the patient's Bruch's membrane, and diagnosing the patient with AMD. A method of detecting the presence of AMD in a patient, by detecting biomarkers in the patient's Bruch's membrane. A method of detecting inflammation in a patient, by detecting biomarkers in the patient's Bruch's membrane. A method of determining the progression of AMD in a patient. A method of determining efficacy of a treatment for AMD in a patient. A method of determining the presence of AMD in an animal model. A kit for detecting the presence of disease in a patient, including an assay for biomarkers 3-nitrotyrosine and nitro-A2E. An assay of the biomarkers. | 2011-10-06 |
20110244505 | Method for In Vitro Blood Testing - Method for determining the presence of a malady in a patient by measuring the degree of reaction of individual types of white blood cells (basophils, eosinophils, neutrophils, monocytes and lymphocytes) in the patient's blood. The degree of reaction of each type of white blood cells is determined by comparing the volumetric size distributions of the types of white blood cells before and after exposure to suspected reactants. Positive results of a change in the volumetric size distribution of the individual types of white blood cells in response to exposure to a specific reactant indicates the presence of a specific malady, such as an allergic reaction to the suspected reactant. | 2011-10-06 |
20110244506 | EGFR DEPENDENT MODULATION OF CHEMOKINE EXPRESSION AND INFLUENCE ON THERAPY AND DIAGNOSIS OF TUMORS AND SIDE EFFECTS THEREOF - The invention relates to diagnosis and therapy of tumors utilizing the epidermal growth factor (EGFR) by means of chemical inhibitors or monoclonal antibodies. The invention relates also to skin irritations, preferably skin rash, in conjunction and associated with the treatment of tumor cells that utilize EGF receptor with anti-cancer agents. The invention is also directed to methods of predicting the efficiency of a tumor therapy/tumor response in a patient based on the treatment with EGFR inhibitors, especially anti-EGFR antibodies. The invention further relates to a method of determining the optimum dose of an anti-cancer agent in EGFR related tumor therapy. | 2011-10-06 |
20110244507 | USE OF NEUREGULIN- AS AN INDICATOR AND/OR TARGET - The invention relates, inter alia, to the use of neuregulin-β as a target in a screening method for active compounds, in particular for exerting an influence on changes in calcium concentration which are mediated by glutamate receptors. | 2011-10-06 |
20110244508 | Method for investigating the thrombocyte function of the blood - A method for investigating the thrombocyte function of the blood, and particularly of platelet aggregation, wherein the following steps are carried out: a) cross-flowing an aperture with blood or blood components; b) determining the active radius of the aperture depending on time and c) evaluating the time-dependent modification of the radius as a measure for blood cell and/or thrombocyte function. | 2011-10-06 |
20110244509 | ApoIII and the Treatment and Diagnosis of Diabetes - The present invention provides methods of identifying candidate compounds for the treatment of type I diabetes comprising contacting pancreatic β cells with an amount of apolipoprotein CIII (“apoCIII”) effective to increase intracellular calcium concentration, in the presence of one or more test compounds, and identifying those test compounds that inhibit an apoCIII-induced increase in intracellular calcium concentration in the pancreatic β cells. The present invention also provides methods for treating patients with type I diabetes comprising administering to the patient an amount effective of an inhibitor of apoCIII to reduce apoCIII-induced increase in intracellular calcium concentration in pancreatic β cells. | 2011-10-06 |
20110244510 | Filtration method for detecting microbial contamination - Disclosed is a convenient sample preparation method for a medium suspected of containing contaminants, the method comprising a) passing a known volume of said medium through a filter from an influent side to an effluent side thereby concentrating the contaminants on the influent side of the filter, b) contacting the influent side of the filter with a liquid vehicle containing at least one substrate that through interaction with the contaminants each produces a detectable moiety, c) and allowing the substrate to interact with the contaminants on the influent side of the filter for a period of time, which is sufficient to allow the detectable moiety to be detected in the liquid vehicle. The method may further comprise a detection step, where the amount of detectable is determined in the liquid vehicle, preferably after the liquid vehicle has been separated from the contaminant, e.g. by passing the liquid vehicle through the filter and performing a measurement on the contaminant free liquid vehicle. Also disclosed is a kit for exercising the inventive method. | 2011-10-06 |
20110244511 | METHODS AND ARTICLES FOR DETECTING HEMOLYTIC MICROORGANISMS - A thin film culture plate device useful for detecting hemolysin-producing microorganisms is included. The device can further include selective agents and/or indicators to differentiate groups or species microorganisms. Methods of use include detecting or enumerating hemolysin-producing microorganisms. | 2011-10-06 |
20110244512 | PENTOSE PHOSPHATE PATHWAY UPREGULATION TO INCREASE PRODUCTION OF NON-NATIVE PRODUCTS OF INTEREST IN TRANSGENIC MICROORGANISMS - Coordinately regulated over-expression of the genes encoding glucose 6-phosphate dehydrogenase [“G6PDH”] and 6-phospho-gluconolactonase [“6PGL”] in transgenic strains of the oleaginous yeast, | 2011-10-06 |
20110244513 | ISOLATED MAMMALIAN MEMBRANE PROTEIN GENES; RELATED REAGENTS - Nucleic acids encoding various lymphocyte cell proteins from mammalian, including primate, reagents related thereto, including specific antibodies, and purified proteins are described. Methods of using said reagents and related diagnostic kits are also provided. | 2011-10-06 |
20110244514 | Mammalian Sweet Taste Receptors - The present invention provides isolated nucleic acid and amino acid sequences of sweet taste receptors comprising two heterologous G-protein coupled receptor polypeptides from the T1R family of sensory G-protein coupled receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of sweet taste receptors. | 2011-10-06 |
20110244515 | METHOD OF PREPARING PROTEIN HAVING HIGH CONTENT OF SPECIFIC AMINO ACID BY CO-EXPRESSION WITH TRNA OF SPECIFIC AMINO ACID - The present invention relates to a method of increasing the expression of a target protein by co-expression of a gene encoding a target protein having a high content of a specific amino acid with a nucleotide sequence encoding the tRNA of the specific amino acid. According to the present invention, the expression of a protein having a high content of a specific amino acid can be remarkably increased by co-expression with the tRNA of the specific amino acid. Thus, the present invention is useful for increasing the productivity of a protein having a high content of a specific amino acid, such as a repetitive protein. | 2011-10-06 |
20110244516 | Rage Fusion Proteins And Methods Of Use - Disclosed are RAGE fusion proteins comprising RAGE polypeptide sequences linked to a second, non-RAGE polypeptide. The RAGE fusion protein may utilize a RAGE polypeptide domain comprising a RAGE ligand binding site and an interdomain linker directly linked to an immunoglobulin C | 2011-10-06 |
20110244517 | SYSTEM FOR ANTIBODY EXPRESSION AND ASSEMBLY - The present invention provides methods and compositions for expression and production of recombinant antibodies in a host cell system, such as prokaryotic and eukaryotic expression systems. Particularly contemplated are recombinant systems for temporally separated expression of light chain and heavy chain of antibodies. The antibody products including antibody fragments can be used in various aspects of biological research, diagnosis and medical treatment. | 2011-10-06 |
20110244518 | Prokaryotic Expression of Soluble, Active Dkk - Dickkopf (Dkk) proteins inhibit the canonical Wnt signaling pathway. Each of the members of the Dkk family has been previously cloned and expressed as a soluble protein in eukaryotic cells, while expression in bacterial cells has resulted in the formation of insoluble inclusion bodies that require further processing. The present invention provides compositions and methods for producing soluble, active dkk protein in prokaryotic host cells, by expressing the dkk protein as a fusion protein with a solubilization molecule, thereby providing an inexpensive and convenient source of pure active Dkk. | 2011-10-06 |
20110244519 | USE OF RNA TRANS-SPLICING FOR ANTIBODY GENE TRANSFER AND ANTIBODY POLYPEPTIDE PRODUCTION - The present invention provides methods and compositions for generating novel nucleic acid molecules through RNA trans-splicing that target a highly expressed pre-mRNA and contain the coding sequence for antibody polypeptide(s). The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact with the target precursor messenger RNA molecule (target pre-mRNA) that is abundantly expressed or tumor specific and mediate a trans-splicing reaction resulting in the generation of novel chimeric RNA molecule (chimeric RNA) capable of encoding an antibody polypeptide. The invention provides for the in vivo production of chimeric RNA molecules that encode and result in the production of an antibody polypeptide that is therapeutically effective against, for example, infectious agents, cancer cells, transplantation antigens, rheumatoid arthritis, etc. | 2011-10-06 |
20110244520 | Compositions and Methods for Bacterial Production of Chondroitin - The invention relates to the field of recombinant DNA technology for the production of chondroitin, including the production of chondroitin sulfate via a combination of recombinant bacterial fermentation and post-fermentation sulfation. | 2011-10-06 |
20110244521 | METHOD FOR PREPARATION OF RECOBINANT DNA - The problem to be solved in the present invention is to provide a simplified and efficiently improved DNA recombination method. | 2011-10-06 |
20110244522 | ROTATIONAL PCR EQUIPMENT AND PCR METHOD USING THE SAME - Provided are a rotational PCR apparatus, a PCR chip for the same and a rotational PCR method using the same. | 2011-10-06 |
20110244523 | Modified RNA Ligase for Efficient 3` Modification of RNA - The invention provides a novel truncated mutated T4 RNA ligase 2. In addition, methods are provided for ligating pre-adenlylated donor molecules to the 3′ hydroxyl group of RNA in the absence of ATP using the ligase. | 2011-10-06 |
20110244524 | CELL-FREE SYSTEM FOR SYNTHESIZING MEMBRANE PROTEINS CELL FREE METHOD FOR SYNTHESIZING MEMBRANE PROTEINS - The invention provides an in vitro method for producing proteins, membrane proteins, membrane-associated proteins, and soluble proteins that interact with membrane-associated proteins for assembly into an oligomeric complex or that require association with a membrane for proper folding. The method comprises, supplying intracytoplasmic membranes from organisms; modifying protein composition of intracytoplasmic membranes from organism by modifying DNA to delete genes encoding functions of the organism not associated with the formation of the intracytoplasmic membranes; generating appropriate DNA or RNA templates that encode the target protein; and mixing the intracytoplasmic membranes with the template and a transcription/translation-competent cellular extract to cause simultaneous production of the membrane proteins and encapsulation of the membrane proteins within the intracytoplasmic membranes. | 2011-10-06 |
20110244525 | MICROORGANISM EXPRESSING XYLOSE ISOMERASE - The present invention relates to a transformed microorganism capable of (a) a higher xylose isomerase activity than the equivalent microorganism prior to transformation; and/or (b) a higher growth rate in or on a growth medium comprising xylose than the equivalent microorganism prior to transformation; and/or (c) a faster metabolism of xylose than the equivalent microorganism prior to transformation; and/or (d) a higher production of ethanol when grown anaerobically on xylose as the carbon source than the equivalent microorganism prior to transformation. | 2011-10-06 |
20110244526 | ALPHA-AMYLASE BLENDS AND METHODS FOR USING SAID BLENDS - The present invention relates to an alpha-amylase blend, including a | 2011-10-06 |
20110244527 | METHOD FOR PRODUCING B-GLUCANASE AND XYLANASE, AND LIQUID CULTURE MEDIUM - A method for producing β-glucanase and xylanase which includes the step of culturing a microorganism classified under the genus | 2011-10-06 |
20110244528 | PROCESS FOR PRODUCING USEFUL SUBSTANCE - The present invention provides a process for efficiently producing a useful substance; and a microorganism which belongs to coryneform bacteria and which can be used in the process. The present invention provides a process for producing a useful substance by using a microorganism belonging to coryneform bacteria, the microorganism having an ability to take a sugar, which is taken into a cell via a phosphotransferase system (PTS), into a cell via a system other than the PTS, and having an ability to produce a useful substance. | 2011-10-06 |
20110244529 | PROCESS FOR THE FERMENTATIVE PREPARATION OF L -ORNITHINE - A process for the fermentative preparation of L-ornithine using microorganisms characterized by an increased export of the amino acid. | 2011-10-06 |
20110244530 | L-SUCCINYLAMINOACYLASE AND PROCESS FOR PRODUCING L-AMINO ACID USING IT - The present invention provides an L-aminoacylase which is able to produce L-tert-leucine being useful as an intermediate for pharmaceuticals. | 2011-10-06 |
20110244531 | SYNTHESIS ROUTES TO 2(S),4(S),5(S),7(S)-2,7-DIALKYL-4-HYDROXY-5-AMINO-8-ARYL-OCTANOYL AMIDES - The invention relates to a process for the preparation of compounds that are important building blocks in convergent synthesis routes to 2(S),4(S),5(S),7(S)-2,7-dialkyl-4-hydroxy-5-amino-8-aryl-octanoyl amides or pharmaceutically acceptable salts thereof, such as the compound Aliskiren, and to a process for the preparation of these octanoyl amides, comprising reacting said building block. | 2011-10-06 |
20110244532 | PRODUCTION OF BRANCHED CHAIN FATTY ACIDS AND DERIVATIVES THEREOF IN RECOMBINANT MICROBIAL CELLS - Recombinant microbial cells are provided which have been engineered to produce branched chain products such as branched fatty acid derivatives by the fatty acid biosynthetic pathway, and methods of making branched fatty acid derivatives using the recombinant microbial cells. | 2011-10-06 |
20110244533 | FIBROUS MATERIALS AND COMPOSITES - Fibrous materials, compositions that include fibrous materials, and uses of the fibrous materials and compositions are disclosed. For example, the fibrous materials can be operated on by a microorganism to produce ethanol or a by-product, such as a protein or lignin. | 2011-10-06 |
20110244534 | PROCESS FOR THE PREPARATION OF A MONOVALENT SUCCINATE SALT - A process for the preparation of a monovalent succinate salt includes:
| 2011-10-06 |
20110244535 | Production Of Peracids Using An Enzyme Having Perhydrolysis Activity - A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided. | 2011-10-06 |
20110244536 | FERMENTIVE PRODUCTION OF ISOBUTANOL USING HIGHLY EFFECTIVE KETOL-ACID REDUCTOISOMERASE ENZYMES - Ketol-acid reductoisomerase enzymes have been identified that provide high effectiveness in vivo as a step in an isobutanol biosynthetic pathway in bacteria and in yeast. These KARIs are members of a clade identified through molecular phylogenetic analysis called the SLSL Clade. | 2011-10-06 |
20110244537 | REGIO- AND ENANTIOSELECTIVE ALKANE HYDROXYLATION WITH MODIFIED CYTOCHROME P450 - Cytochrome P450 BM-3 from | 2011-10-06 |
20110244538 | FERMENTATION OF GASEOUS SUBSTRATES - The invention relates to the microbial fermentation of gaseous substrates, particularly to gas/liquid contact modules and bioreactors configured to improve the efficiency of fermentations, particularly microbial fermentations of substrates comprising CO. In a particular embodiment, a gas/liquid contact module with multiple channels is configured to produce products in a liquid fermentation broth. In a further particular embodiment, there is provided a method of fermentation of a gaseous substrate to produce a product in a liquid fermentation broth. | 2011-10-06 |
20110244539 | Selection Of Cellulolytic Microbes With High Growth Rates - Methods for obtaining cellulolytic microbes with high growth rates are disclosed. For example, | 2011-10-06 |
20110244540 | Preservation of Methanogenic, Hydrogen-Utilizing Microbial Cultures - Disclosed are drying methods for preserving cultures of methanogens and dried cultures obtained by the disclosed drying methods. The dried cultures of methanogens may be reconstituted and utilized to bioaugment anaerobic digester systems. | 2011-10-06 |
20110244541 | Bioaugmentation of Anaerobic Digester Systems - Disclosed herein are methods for improving performance of an anaerobic digester system. The methods typically include adding a culture comprising hydrogenotrophic methanogens to the system, otherwise referred to as bioaugmentation. | 2011-10-06 |
20110244542 | CLOSTRIDIUM SPOROSPHAEROIDES FOR THE TREATMENT OF BIOMASS - A method of treatment of biomass is described, characterized in that a microorganism of the species | 2011-10-06 |
20110244543 | U-Shape And/or Nozzle U-Loop Fermenter And Method Of Fermentation - A fermenter and a method of fermentation in a U-shape and/or nozzle U-loop fermenter ( | 2011-10-06 |
20110244544 | Polymeric carriers for immunohistochemistry and in situ hybridization - Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays. | 2011-10-06 |
20110244545 | Polymeric carriers for immunohistochemistry and in situ hybridization - Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays. | 2011-10-06 |
20110244546 | Internalizing Anti-CD74 Antibodies and Methods of Use - The present invention provides humanized, chimeric and human anti-CD74 antibodies, CD74 antibody fusion proteins, immunoconjugates, vaccines and bispecific that bind to CD74, the major histocompatibility complex (MHC) class-II invariant chain, Ii, which is useful for the treatment and diagnosis of B-cell disorders, such as B-cell malignancies, other malignancies in which the cells are reactive with CD74, and autoimmune diseases, and methods of treatment and diagnosis. | 2011-10-06 |
20110244547 | SULFOTRANSFERASE 1E1 SEQUENCE VARIANTS - Isolated sulfotransferase nucleic acid molecules that include a nucleotide sequence variant and nucleotides flanking the sequence variant are described, as well as sulfotransferase allozymes. Methods for determining if a mammal is predisposed to cancer also are described. | 2011-10-06 |
20110244548 | DNA Polymerases Having Improved Labeled Nucleotide Incorporation Properties - The present invention relates to mutant DNA polymerases that exhibit reduced discrimination against labeled nucleotides into polynucleotides. The DNA polymerases of the invention have at least one mutation in the nucleotide label interaction region of the enzyme such the mutation results in reduced discrimination against labeled nucleotides. The nucleotide label interaction regions is located at portions of the O-helix, (ii) the K helix, and (iii) the inter O-P helical loop of Taq DNA polymerase or analogous positions in other DNA polymerases. In addition to providing novel mutant DNA polymerases, the invention also provides polynucleotides encoding the subject mutant DNA polymerases. The polynucleotides provided may comprise expression vectors for the recombinant production of the mutant polymerases. The invention also provides host cells containing the subject polynucleotides. The invention also includes numerous methods of using the subject DNA polymerases, including uses for chain termination sequencing and PCR. Another aspect of the invention is to provide kits for synthesizing fluorescently labeled polynucleotides in accordance with the methods of the invention. Kits of the invention comprise a mutant DNA polymerase of the invention and a fluorescently labeled nucleotide that exhibits reduced discrimination with respect to the mutant DNA polymerase in the kit. | 2011-10-06 |