31st week of 2010 patent applcation highlights part 47 |
Patent application number | Title | Published |
20100196875 | HUMAN ERYTHROVIRUS - Nucleic acid molecules derived from sequences of novel human parvovirus B19 variant genomes are provided. Also provided are assays and kits comprising the nucleic acid molecules. | 2010-08-05 |
20100196876 | Methods and Reagents for Genotyping HCV - The present invention is directed to methods and reagents for determining the genotype of a hepatitis C virus (HCV) species present in a test sample. The invention more particularly relates to mixtures of degenerate amplification and sequencing primers, and methods of using such primers, that are complementary to a plurality of HCV species, and are capable of generating nucleotide sequence information for a region of NS5B of HCV that is, for each species, indicative of the type and/or subtype, of the species present in the sample. | 2010-08-05 |
20100196877 | METHOD OF DETECTION OF MICROORGANISMS WITH ENHANCED BACTERIOPHAGE AMPLIFICATION - A method of determining the presence or absence of a target microorganism in a sample to be tested, the method comprising: combining with the sample an amount of bacteriophage capable of attaching to the target microorganism to create a bacteriophage exposed sample, and a substance which enhances bacteriophage amplification or sensitivity; providing conditions to the bacteriophage-exposed sample sufficient to allow the bacteriophage to infect the microorganism; and assaying the bacteriophage-exposed sample to detect the presence or absence of a bacteriophage marker to determine the presence or absence of the target microorganism. | 2010-08-05 |
20100196878 | DUAL-RECOGNITION IMMUNOASSAY FOR THE DETECTION OF ANTIBODIES - The invention relates to a dual-recognition immunoassay for the detection of antibodies specific to a target antigen in a sample which comprises contacting said target antigen with a sample suspected of containing said antibodies specific to said target antigen under conditions allowing the formation of an antigen-antibody complex; adding a conjugate comprising said target antigen and a marker under conditions allowing the formation of an antigen-antibody-antigen/marker complex; and detecting said antigen-antibody-antigen/marker complex. The immunoassay can be used, among other applications, in the diagnosis of infections caused by pathogenic organisms with high sensitivity and specificity. | 2010-08-05 |
20100196880 | COMPOSITIONS AND MULTIPLEX ASSAYS FOR MEASURING BIOLOGICAL MEDIATORS OF PHYSIOLOGICAL HEALTH - Multiplex assays are provided including panels of probes for development of multiplex assays capable of simultaneously measuring multiple biologically-relevant proteins using very small quantities of biological samples to rapidly assess the health status of animals, especially companion animals, as well as to formulate nutritional regimens for improving the health status of animals. The probes are provided as are methods for using them to assess the health status of animals, as well as their responses to therapeutic or nutritional interventions therein. | 2010-08-05 |
20100196881 | REVERSE RESTRICTION FRAGMENT LENGTH POLYMORPHISM ASSAY AND USES THEREOF - The present invention presents a Reverse Restriction Fragment Length Polymorphism (RRFLP) method for the detection of the presence of an informative restriction enzyme site in a nucleotide sequence. The method includes digesting a sample with the informative restriction enzyme; performing polymerase chain reaction (PCR) on the digested sample with an oligonucleotide primer pair that flanks the informative restriction enzyme site; determining the Ct value of the sample; comparing the Ct value of the sample to the Ct value from a control sample; and calculating a ΔCt value, wherein a ΔCt value is the Ct value of the sample minus the Ct value of a control; and wherein a ΔCt value ≧+1 indicates that the informative restriction enzyme sites is present in the nucleotide sequence. The present invention includes the application of the RRFLP method for detection of the infectious laryngotracheitis virus (ILTV). | 2010-08-05 |
20100196882 | GALECTIN-3 nsSNP MARKER FOR CANCER - A diagnostic biomarker including a mechanism for determining a patient's propensity to develop cancer. A diagnostic kit for determining a patient's propensity to develop cancer. A method of predicting both a patient's and a population's propensity to develop cancer, by detecting the presence of an H | 2010-08-05 |
20100196883 | ANDROGEN-REGULATED GENES AND USES FOR DIAGNOSIS, PROGNOSIS AND TREATMENT OF PROSTATE NEOPLASTIC CONDITIONS - The invention provides a method for diagnosing or predicting susceptibility to a prostate neoplastic condition in an individual. The method involves (a) determining a level of RDC1 in a sample from the individual, and (b) comparing the level of RDC1 in the sample to a reference level of RDC1, wherein a level of RDC1 in the sample 2-fold or more higher than the reference level indicates the presence of, or susceptibility to, a prostate neoplastic condition in the individual. | 2010-08-05 |
20100196884 | Nucleic Acid Preparation - The present invention is directed to methods, devices and computer programs for preparing nucleic acids from a template nucleic acid by subjecting a sample to thermocycles. After a first number of thermocycles, a partial amount of the reaction mixture is being subjected to a second number of thermocycles. This two step amplification method speeds up overall reaction time without affecting the limit of detection. | 2010-08-05 |
20100196885 | METHODS OF VALIDATING CANDIDATE COMPOUNDS FOR USE IN TREATING COPD AND OTHER DISEASES - The present invention relates to methods of diagnosing and treating elastin fiber injuries. In additional preferred embodiments, the present invention relates to methods of validating candidate compounds for use in treating chronic obstructive pulmonary disease (COPD), chronic bronchitis, emphysema, refractory asthma, and other related diseases. Examples of such methods include determining if the candidate compound decreases the degradation of elastic fiber in a patient administered the candidate compound by measuring, using mass spectrometry, a marker of elastic fiber degradation in a sample of a body fluid or a tissue of the patient. The invention provides that a decrease in the presence of the marker compared to a control validates that the candidate compound is effective to treat, prevent, or ameliorate the disease. | 2010-08-05 |
20100196886 | SCREENING, DIAGNOSING, TREATING AND PROGNOSIS OF PATHOPHYSIOLOGIC STATUS BY RNA REGULATION - A relationship between cancer and ribonucleic acid (RNA) regulation is described by determining intracellular levels of niRN A regulators. Generally, mRNA levels are decreased in cancer cells that may be a reflection of either reduced mRNA expression and/or increased mRNA degradation. miRNAs are identified that hybridize to an mRNA that are suspected to mediate intracellular mRNA steady state levels. Alternatively, ribonucleic acid binding protein (RBP) levels may also mediate intracellular mRNA steady state levels. In particular, this invention demonstrates an effective clinical management strategy for uterine cell cancers may be implemented by taking advantage of an exemplary relationship between P2X | 2010-08-05 |
20100196887 | COMPOSITIONS AND METHODS FOR MULTIPLEX ANALYSIS OF POLYNUCLEOTIDES - Provided herein are compositions and methods for the multiplex analysis and/or detection of polynucleotides having one or more distinguishable target sequences. The methods employ signal-quencher probe pairs having specific relative differential thermal melting temperatures that permit the detection of one or more target sequences on one or more polynucleotides. | 2010-08-05 |
20100196888 | Genotyping Using Multiple Variant-Specific Primer Pools - This invention relates to the identification of variants of an organism using variant-specific oligonucleotide primer pools to specifically hybridize to those polynucleotides in an sample comprising a plurality of polynucleotides that contain a target sequence that is unambiguously identifiable with a particular variant of the organism. | 2010-08-05 |
20100196889 | Gene Expression Profiling for Identification, Monitoring and Treatment of Colorectal Cancer - A method is provided in various embodiments for determining a profile data set for a subject with colorectal cancer or conditions related to colorectal cancer based on a sample from the subject, wherein the sample provides a source of RNAs. The method includes using amplification for measuring the amount of RNA corresponding to at least 1 constituent from Tables 1-5. The profile data set comprises the measure of each constituent, and amplification is performed under measurement conditions that are substantially repeatable. | 2010-08-05 |
20100196890 | PRIMERS FOR MELTING ANALYSIS - Methods and kits are provided for nucleic acid analysis. In an illustrative method a target nucleic acid is amplified using a first primer and a second primer, wherein the first primer comprises a probe element specific for a locus of the target nucleic acid and a template-specific primer region, and the probe element is 5′ of the template-specific primer region, subsequently allowing the probe element to hybridize to the locus to form a hairpin, generating a melting curve for the probe element by measuring fluorescence from a dsDNA binding dye as the mixture is heated, wherein the dye is not covalently bound to the first primer, and analyzing the shape of the melting curve. Kits may include one or more of the first and second primers, the dsDNA binding dye, a polymerase, and dNTPs. | 2010-08-05 |
20100196891 | IBC-1 (Invasive Breast Cancer-1), a Putative Oncogene Amplified in Breast Cancer - This invention encompasses antibodies specific for IBC-1 (Invasive Breast Cancer-1), methods for diagnosis and prognosis of metastatic breast cancer and degenerative neural conditions, methods of identifying and manufacturing therapeutic compounds, and methods of treating patients with invasive and metastatic breast cancer or degenerative neural conditions. | 2010-08-05 |
20100196892 | Methods and Systems for Molecular Fingerprinting - This invention relates in general to a method for molecular fingerprinting. The method can be used for forensic identification (e.g. DNA fingerprinting, especially by VNTR), bacterial typing, and human/animal pathogen diagnosis. More particularly, molecules such as polynucleotides (e.g. DNA) can be assessed or sorted by size in a microfabricated device that analyzes the polynucleotides according to restriction fragment length polymorphism. In a microfabricated device according to the invention, DNA fragments or other molecules can be rapidly and accurately typed using relatively small samples, by measuring for example the signal of an optically-detectable (e.g., fluorescent) reporter associated with the polynucleotide fragments. | 2010-08-05 |
20100196893 | Method for genotyping DNA tandem repeat sequences - The present invention provides methods for determining the number of tandem repeat units in a region of double stranded DNA based on the use of RecA-like recombinase protein and oligonucleotide ligation. The methods of the present invention provide RecA coated, specific DNA oligonucleotide probes (RecA filaments) for homology searching in duplex DNA where the location of homologous sequences results in the formation of D-loop structures containing a duplex region comprising the oligonucleotide probe and one strand of the target DNA. The present invention further provides sets of oligonucleotide probes (ligation partners) selected to have sequence complementary to non-repeat sequence flanking a region of tandem repeats and sequence complementary to varying numbers of repeat units such that only that pair of oligonucleotides that can be aligned, via RecA mediated homology searching, with the target sequence such that their terminal bases are paired with adjacent nucleotides in the target sequence will be substrates for ligation. Thus, the present invention provides methods whereby successful ligation is diagnostic of the number of repeat units in the target DNA sequence. Also disclosed are compositions and kits useful for practicing the foregoing methods. | 2010-08-05 |
20100196894 | PROCEDURE FOR THE DETECTION OF PARATUBERCULOSIS - The present invention relates to a procedure for the specific and sensitive detection of | 2010-08-05 |
20100196895 | METHOD FOR DETERMINATION OF ONSET RISK OF GLAUCOMA - A method of determining the presence or the absence of a glaucoma risk, including the steps of detecting in vitro an allele and/or a genotype of a single nucleotide polymorphism which is located on a 31st base of a base sequence, in a sample from a subject, wherein the base sequence is at least one base sequence selected from the group consisting of base sequences shown in SEQ ID NOs: 203 to 514 or a complementary sequence thereto (step A), and comparing the allele and/or the genotype detected in the step A with at least one of an allele and/or a genotype, containing a high-risk allele, in the base sequences shown in SEQ ID NOs: 203 to 514 (step B). According to the method of the present invention, the level of an onset risk of glaucoma in a sample donor can be determined by analyzing an allele or a genotype of a single nucleotide polymorphism in the present invention on the sample, so that the sample donor can take a preventive measure of glaucoma, or can receive appropriate treatments, on the basis of this risk. | 2010-08-05 |
20100196896 | METHOD AND A KIT FOR IDENTIFYING A HUMAN WHO HAS THE PREDISPOSITION FOR INCREASED CONSUMPTION OF CARBOHYDRATES AND METHOD FOR MANAGING THE NAMED HUMAN'S DIETARY INTAKE OF NUTRITIONAL ENERGY - A method and a kit for determining the human with genetic predisposition to increased consumption of carbohydrates and to develop metabolic, psychiatric or neurological disease or disorder, or obesity. The invention pertains to the detection of a human's ADRA2A genotype, and if there is detected the homozygous nucleotide G at the position C-1291 of the ADRA2A gene, then the patient possesses a predisposition to increased consumption of carbohydrates and to develop metabolic, psychiatric or neurological diseases or disorders or obesity, and there is a need to decrease intake of carbohydrates. | 2010-08-05 |
20100196897 | Method and Device for Non-Invasive Prenatal Diagnosis - Method for non-invasive prenatal diagnosis comprising the following steps: a. obtain a sample of an organic fluid having a high probability of containing foetal cells from a pregnant woman; b. enrich said sample of organic fluid in at least one population of cells comprising at least one type of foetal nucleated cells; c. isolate at least one cell from among said at least one type of foetal nucleated cells; d. perform a genetic analysis on said at least one cell isolated from among said at least one type of foetal nucleated cells in order to highlight at least one genetic characteristic of said at least one foetal nucleated cell suitable for permitting said diagnosis; wherein the step of isolating at least one cell from among said at least one type of foetal nucleated cells is performed by individually selecting single cells in a microfluidic device designed for said purpose. | 2010-08-05 |
20100196898 | DISEASE-ASSOCIATED GENETIC VARIATIONS AND METHODS FOR OBTAINING AND USING SAME - The invention provides a comprehensive, rapid, unbiased, and accurate method for identifying and/or discovering disease-associated genetic variations, e.g., disease-associated variations. The present invention further provides novel disease-associated genetic variations for use as genetic markers of disease, e.g., cancer. The invention further provides methods for assessing an individual's risk for developing a disease, e.g., cancer, by detecting the presence the novel disease-associated genetic variations of the invention. | 2010-08-05 |
20100196899 | Systems for genome selection - Systems, methods, compositions and apparatus relating to genome selection are disclosed. | 2010-08-05 |
20100196900 | METHOD OF DETECTING CANINE EXERCISE-INDUCED COLLAPSE - The present invention relates to diagnosing Canine Exercise-Induced Collapse (EIC). | 2010-08-05 |
20100196901 | Method for Obtaining Information on Biological Rhythm by Using Hair - To provide a simple and low-invasive method for obtaining information on a biorhythm of an individual organism. Provided is a method for obtaining information on a biorhythm of an individual organism based on variations over time in an expression level of a clock gene in a hair follicle cell of the individual organism. According to this method, a phase shift of the biorhythm of the individual organism can be detected by preparing a plurality of times about the individual organism and conducting a collation. Further, a phase shift of the biorhythm of the individual organism can also be detected by collating an expression level of the clock gene at a predetermined time with the molecular clock table. | 2010-08-05 |
20100196902 | PROSTATE CANCER BIOMARKERS - Disclosed are biomarkers, at least, useful for the diagnosis and/or prognosis of cancer and for making treatment decisions in cancer, for example prostate cancer. | 2010-08-05 |
20100196903 | COMPOSITIONS AND METHODS TO DETECT TMPRSS2/ERG TRANSCRIPT VARIANTS IN PROSTATE CANCER - Compositions and methods for detecting TMPRSS2/ERG transcript variants in prostate cancer are provided. The compositions and methods have utility in prostate cancer diagnosis. | 2010-08-05 |
20100196904 | AMBIENT TEMPERATURE STABLE KITS FOR MOLECULAR DIAGNOSTICS - A method for processing DNA polymerase and/or dNTPs for use in an amplification procedure, includes providing a solution mixture, the solution mixture including a DNA polymerase and/or dNTPs, a buffer solution and at least one stabilizing agent and hydration reducing the solution mixture. The solution mixture is hydration reduced at a temperature between 0° C. and about 100° C. | 2010-08-05 |
20100196905 | Adult Stem Cell Assays - Provided are methods for quantifying adult stem cells in a test sample, methods of quantifying adult stem cells in a patient, and methods for quantifying differentiated cells in a test sample. Also provided are kits for quantifying adult stem cells or differentiated cells in a sample. | 2010-08-05 |
20100196906 | Accelerated Progression Relapse Test - Disclosed is an accelerated progression relapse test for use in the prognosis of disease states. According to the test disclosed herein, it can be determined whether a patient would benefit from treatment for a disease state or whether the patient's prognosis would not have a high probability of benefit with additional treatment. In particular, the test is useful in determining a patient's prognosis for cancer (breast, colon, lung, etc). For example, the test of the invention can be used to determine the prognosis for estrogen receptor positive (ER+) breast cancer patients. In the test for ER+ breast cancer patients, four genetic probes are employed that target MK167, CDC6, and SPAG5 gene products. The ER+ breast cancer test stratifies a patient population into two groups, with the low gene expression group identifying a group that is less likely to benefit from additional treatment measures, and a high gene expression group, identifying a group more likely to benefit from additional treatment measures. | 2010-08-05 |
20100196907 | MARKERS TO PREDICT AND MONITOR RESPONSE TO AURORA KINASE B INHIBITOR THERAPY - The present invention relates to identifying the presence or absence of one or more copy number gains in the ABCB1 gene, the ABCB4 gene or combinations thereof, identifying patients eligible to receive Aurora kinase inhibitor therapy, either as monotherapy or as part of combination therapy, and monitoring patients' response to such therapy. | 2010-08-05 |
20100196908 | SYSTEMS AND METHODS FOR DETECTING A SIGNAL AND APPLYING THERMAL ENERGY TO A SIGNAL TRANSMISSION ELEMENT - A signal detection system configured for detecting a signal emitted by the contents of a reaction receptacle is also configured to apply thermal energy to a portion of the reaction receptacle to affect a reaction occurring within the reaction receptacle. More particularly, a system for detecting electromagnetic radiation emitted by the contents of a reaction receptacle includes a transmission element configured for transmitting electromagnetic radiation from the contents of the receptacle, a thermal element associated with the transmission element and configured to apply thermal energy to at least a portion of the receptacle, and a detector configured to receive electromagnetic radiation from the transmission element and to generate a signal corresponding to a characteristic of the electromagnetic radiation received by the detector. | 2010-08-05 |
20100196909 | PARP-BASED CYTOCHEMICAL AND HISTOCHEMICAL DETECTION METHODS AND KITS THEREFORE - The present invention relates to new cytochemical and histochemical detection methods based on the enzymatic activity of poly-ADP-ribose-polymerases (PARP). In particular, the present invention provides methods and systems for cytochemical or histochemical detection of a molecule of interest based on detecting poly-ADP-ribose (PAR). Further, the present invention provides methods for screening for compounds altering the amount of NAD or analogs thereof in subcellular compartments based on detecting the amount of PAR formed in said subcellular compartment as well as methods for determining the spatial distribution of a molecule of interest. In a further aspect, the present invention provides systems and kits for conducting said methods as well as a new expression vectors suitable for said systems. | 2010-08-05 |
20100196910 | METHODS OF GENERATING HUMAN CARDIAC CELLS AND TISSUES AND USES THEREOF - A method of generating cells predominantly displaying at least one characteristic associated with a cardiac phenotype is disclosed. The method comprises (a) partially dispersing a confluent cultured population of human stem cells, thereby generating a cell population including cell aggregates; (b) subjecting the cell aggregates to culturing conditions suitable for generating embryoid bodies; (c) subjecting the embryoid bodies to culturing conditions suitable for inducing cardiac lineage differentiation in at least a portion of the cells of the embryoid bodies, the culturing conditions suitable for inducing cardiac lineage differentiation including adherence of the embryoid bodies to a surface, and culture, medium supplemented with serum, thereby generating cells predominantly displaying at least one characteristic associated with a cardiac phenotype. | 2010-08-05 |
20100196911 | AUTOMATED IDENTIFICATION OF GENETIC TEST RESULT DUPLICATION - The present invention relates to a method and system for determining whether an ordered genetic test will duplicate at least one existing genetic test result. The method includes receiving an ordered genetic test that is for a particular genetic sequence or variant thereof for an individual. The method further includes determining whether the individual has at least one existing genetic test result for the particular genetic sequence or variant thereof. Upon determining that the individual has at least one existing genetic test result for the particular genetic sequence or variant thereof, a notification of the existence of the at least one existing genetic test result is provided. | 2010-08-05 |
20100196912 | Polyelectrolyte-Coated Size-Exclusion Ion-Exchange Particles - A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided. | 2010-08-05 |
20100196913 | Mutant AOX1 Promoters | 2010-08-05 |
20100196914 | RARE CELL DETECTION USING FLAT-PANEL IMAGER AND CHEMILUMINESCENT OR RADIOISOTOPIC TAGS - Disclosed is a method using a large area flat panel imager which is specifically adapted for rare cell detection methods. The method generally includes an imager having a sample receiving surface which can provide a digital or electronic image of a sample deposited on the surface. The method also includes a selectively positionable microscope and/or camera which are used to obtain high resolution images of the deposited samples. An electronic controller can also be used in conjunction with the imager, microscope, and/or camera to selectively position at least one of those components to focus on desired regions of the deposited sample. The noted method is particularly adapted for use with chemiluminescence or other tagging technologies. | 2010-08-05 |
20100196915 | Method of Detecting Oncogenesis of Hematopoietic Cells - Disclosed in a method of detecting cancer using IL-27 receptors. IL27R is a cytokine receptor identified as a novel oncogene from an acute myeloid leukemia patient. It induces cancer-like properties when expressed in cells and can activate a protein that causes various myeloid cell disorders. The data show cytokine receptors play unappreciated roles in mediating activation of signaling pathways in circulatory system cancers. Also method of screening for novel oncogenes using a functional, approach is disclosed using cytokine-dependent cells to screen for transforming events. | 2010-08-05 |
20100196916 | Drug Screening using Islet Cells and Islet Cell Progenitors from Human Embryonic Stem Cells - This disclosure provides a system for producing pancreatic islet cells from embryonic stem cells. Differentiation is initiated towards endoderm cells, and focused using reagents that promote emergence of islet precursors and mature insulin-secreting cells. High quality populations of islet cells can be produced in commercial quantities for use in research, drug screening, or regenerative medicine. | 2010-08-05 |
20100196917 | CELL ANALYSIS APPARATUS AND CELL ANALYSIS METHOD - A cell analysis apparatus that can accurately distinguish between an aggregating cell and a non-aggregating cell is provided. The cell analysis apparatus ( | 2010-08-05 |
20100196918 | ANALYTE SENSORS, METHODS FOR PREPARING AND USING SUCH SENSORS, AND METHODS OF DETECTING ANALYTE ACTIVITY - Embodiments of the present disclosure provide for analyte sensors, methods for producing and using the analyte sensors, methods of detecting and/or measuring analyte activity, methods for characterizing analyte cellular activity, methods of detecting pH change in a system, method of controlling the concentration of an analyte in a system, fusion proteins, polynucleotides, and vectors corresponding to the analyte sensors, kits, and the like. | 2010-08-05 |
20100196919 | PEPTIDES, ANTIBODIES AND FRAGMENTS THEREOF FOR THE TREATMENT AND DIAGNOSTIC OF CELIAC DISEASE - The present invention discloses a peptide having an amino acid sequence comprising an epitope recognized by Igs of patients with active celiac disease and not recognized by Igs of patients on gluten-free diet, antibodies directed against this peptide and methods for diagnosing the celiac disease in a subject, or to check the effect of a therapy or of a diet treatment in a celiac disease affected subject, or to assess the risk of developing celiac disease in a subject vaccinated with a Rotavirus VP7 protein. | 2010-08-05 |
20100196920 | NANOSCOPIC BIOMOLECULAR ABSORPTION SPECTROSCOPY ENABLED BY SINGLE NANOPARTICLE PLASMON RESONANCE ENERGY TRANSFER - The disclosure provides methods and compositions useful for measuring a target analyte in a sample with nanoparticle plasmon resonance. In particular the disclosure provides methods and compositions for measuring a target analyte comprising plasmon resonance energy transfer. | 2010-08-05 |
20100196921 | Method for Predicting the Outcome of a Critically Ill Patient - The present invention relates to a method for predicting the outcome of a critically ill patient, said method comprising measuring the concentration of Chromogranin A or a fragment thereof in a biological sample obtained from said patient. | 2010-08-05 |
20100196922 | FLUORESCENT METAL ION INDICATORS WITH LARGE STOKES SHIFTS - The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength. | 2010-08-05 |
20100196923 | PLURIPOTENT ADULT STEM CELLS - Disclosed herein are pluripotent adult stem cells and methods of use thereof. The cells are found in, or collected from, an adult tissue or fluid. In some embodiments, the cells are c-kit positive and SSEA-4 positive, and can he differentiated into multiple tissue types, e.g., adipogenic, osteogenic, myogenic, endothelial, neurogenic and hepatic tissues. | 2010-08-05 |
20100196924 | Method for Diagnosing Tumors - The present invention describes a method for diagnosing tumors of the reproductive organs which is characterised by determination of the afamin content in a sample of a body fluid or in a tissue sample, wherein a tumour is diagnosed if the afamin content in the sample is decreased compared to the afamin content in a sample from a person without a tumour of the reproductive organs. | 2010-08-05 |
20100196925 | Methods for Screening Cells and Antibodies - The invention provides methods of detecting a change in cell growth patterns, methods of screening many different antibodies in one receptacle, and methods of detecting specific binding of an antibody to a protein or cell, wherein the antibody is in a mixture of many different antibodies. | 2010-08-05 |
20100196926 | Combined Method And Kit For The Sequential Measurement of (1) The Enzymatically Active Fraction And (2) The Total Amount Of An Enzyme - The present invention is related to a combined method and kit (or device) for the sequential measurement of the enzymatically active fraction and the total amount of an enzyme [(such as myeloperoxidase (MPO)] in a sample, and that find improved applications in veterinary and human health fields. | 2010-08-05 |
20100196927 | METHOD OF DETERMINING FUNCTIONAL DEFICIENCIES IN THE COMPLEMENT SYSTEM - The present invention relates to a method of in vitro determining functional deficiencies in the lectin pathway of the complement system, the method comprises the steps of (a) diluting a mammalian sample of body fluid with a diluent comprising one or more inhibitors of the activation of the classical and the alternative pathways of the complement system; (b) activating the lectin pathway of the complement system in the sample obtained from (a); and (c) determining in the sample obtained from (b) the activation of one or more of the complement factors C3, C4, or one or more of the components of the C5-C9 complex. The invention furthermore relates to kits for use in connection with the above-mentioned method, the first kit comprises i) a first component comprising a carrier, one or more inhibitors of the classical and the alternative complement pathways and a diluent; and ii) a second component comprising one or more substances for activation of the lectin complement pathway and optionally an inert carrier. The second kit comprises a container comprising a predetermined amount of one or more inhibitors of the classical and the alternative complement pathways and a diluent, wherein the container is adapted for receiving a predetermined amount of sample, so that when the predetermined amount of sample is added, the concentration of the one or more inhibitors is an inhibitory effective concentration of the classical and alternative pathways, but not the lectin pathway. | 2010-08-05 |
20100196928 | Gene specific to cancer and diagnosis kit using the same - The present invention provides a new additional identification of a gene related to cancer expression and a diagnostic kit using the same. | 2010-08-05 |
20100196929 | STABILISATION OF THE PHENOTYPIC PROPERTIES OF ISOLATED PRIMARY CELLS - The invention provides a method for stabilisation of phenotypic properties of isolated primary cells comprising modulating at least two epigenetic modifications in said cells. | 2010-08-05 |
20100196930 | CELL BASED ASSAYS FOR THE TRIGLYCERIDE SYNTHESIS PATHWAY - A method for identifying a compound which modulates the activity of acyl-coA: diacylglycerol acyltransferase comprises the steps of contacting a stable isotope labeled fatty acid with cells in either presence or absence of the compound, extracting the cells with isopropyl alcohol, and determining the level of a stable isotope labeled triglyceride in the presence or absence of the compound; wherein a change in the level of the stable isotope labeled triglyceride indicates that the compound modulates the DGAT activity. | 2010-08-05 |
20100196931 | DETERMINATION OF RESPONDERS TO CHEMOTHERAPY - The present invention relates to a method of determining whether a biological sample comprising human lung cancer cells is sensitive to a combination of an epidermal growth factor receptor inhibitor and a chemotherapeutic agent by determining the overexpression of a phosphorylated AKT protein and/or a phosphorylated MAPK protein in the biological sample. The invention is also related to methods for deriving a candidate agent or for selecting a composition for inhibiting the progression of lung cancer in a patient wherein a phosphorylated AKT protein and/or a phosphorylated MAPK protein is used. | 2010-08-05 |
20100196932 | Yeast Reporter System - Cell-based screens for compounds that inhibit the accumulation of amyloids are described. For example, the herein described screens may be used to assay the formation, or inhibition, of pre-amyloid or amyloid aggregates and/or oligomers. Agents have been identified that interfere with, or inhibit, the oligomerization of the major component of amyloid plaques; a | 2010-08-05 |
20100196933 | Recombinant Polypeptides for Diagnosing Infection with Trypanosoma Cruzi - Recombinant polypeptides are disclosed that are useful for diagnosing American trypanosomiasis, or Chagas disease, a disease caused by the infectious agent | 2010-08-05 |
20100196934 | PRION ASSAY - The invention relates to an assay method for detecting the presence of PrP | 2010-08-05 |
20100196935 | TREATING PRE-ECLEMPSIA AND CARDIOVASCULAR DISEASES - This document relates to methods and materials involved in identifying, predicting, and treating pre-eclampsia or a cardiovascular disease (e.g., atherosclerosis) in mammals. For example, methods and materials involved in using serum levels of HtrA polypeptides to identify pre-eclampsia or a cardiovascular disease, using HTRA polymorphisms to predict pre-eclampsia or a cardiovascular disease, and using HtrA antibodies to treat pre-eclampsia or a cardiovascular disease in mammals (e.g., humans) are provided. | 2010-08-05 |
20100196936 | METHOD AND KIT FOR DETECTING CANCER - A method for detecting cancer is disclosed. The method includes the steps of: (1) obtaining a test sample of body fluid from a subject; (2) measuring a calsyntenin-1 (CLSTN-1) level in the test sample of body fluid in vitro; and determining if the CLSTN-1 level in the test sample of body fluid is significantly higher than a normal CLSTN-1 level in a sample of body fluid from a healthy population of the same species as the subject, and if yes, indicating that the subject under test has cancer. Methods for monitoring therapeutic efficacy of a therapy or a medicament for treating a cancer patient and for identifying efficacy of a medicament in treatment of cancer are disclosed, and a kit for use in the aforesaid methods is also provided. | 2010-08-05 |
20100196937 | CASCADE ENZYME-LINKED IMMUNOSORBENT ASSAY - The present invention relates to a cascade enzyme-linked immunosorbent assay, more precisely a cascade enzyme-linked immunosorbent assay using magnetic microparticles (MMPs) immobilized with the target antigen specific primary antibody and silica nanoparticles (SPs) immobilized with a cascade reaction initiator and the antigen-specific secondary antibody. When the method of the present invention is applied in the detection of an antigen in biosamples, the detection sensitivity can be significantly increased. | 2010-08-05 |
20100196938 | IMMUNOASSAY FOR VENOM DETECTION INCLUDING NONINVASIVE SAMPLE COLLECTION - Methods and immunoassays for diagnosing a bite or sting of a venomous organism in a patient having symptoms consistent with such a bite or sting are provided. A sample of venom is collected from the area of the suspected bite or sting using a swab and then contacted with an antibody that specifically binds to an antigenic site on venom present in the sample. Binding is then detected. The invention is illustrated by examples showing diagnosis of brown recluse spider bite, distinguishing it from other diagnoses with which it is often confused. This extremely sensitive test can detect venom antigens down to about 20 picograms even after the sample has been shipped and stored for periods of up to three weeks during the summer. | 2010-08-05 |
20100196939 | Diagnosis of Conditions Associated with Decreased Arginine Bioavailability - The invention features methods and compositions for diagnosis, including prognosis, of conditions associated with decreased arginine bioavailability (which can result from dysregulated arginine metabolism, e.g., due to increased arginase activity) by assessing in a sample from a subject the ratio of arginine to one or more, usually two or more, modulators of arginine bioavailability. In one embodiment, the ratio of arginine to (ornithine+citrulline) is assessed to aid in diagnosis. | 2010-08-05 |
20100196940 | METHODS RELATED TO CELL SURFACE GLYCOSYLATION - The present disclosure provides methods for assessing the glycosylation of a target glycoprotein produced by a cell through analysis of cell-surface glycans on the cell. The present disclosure therefore teaches that glycosylation of cell surface proteins can serve as a proxy for glycosylation of other proteins. | 2010-08-05 |
20100196941 | Multi-Analyte Analysis of Saliva Biomarkers as Predictors of Periodontal and Pre-Implant Disease - The present invention relates to methods of measuring biomarkers to determine the probability of a periodontal and/or peri-implant disease. More specifically, the invention provides a panel of biomarkers that, when used in combination, can allow determinniation of the probability of a periodontal and/or peri-implant disease state with extremely high accuracy. | 2010-08-05 |
20100196942 | BIOMARKERS OF LIVER INJURY - Novel, sensitive and specific markers for diagnostics and monitoring of liver injuries, including, but not limited to ischemic liver damage, are provided. This includes identification several enzymes of arginine/urea/nitric oxide cycle, sulfuration enzymes and spectrin breakdown related products, among others. | 2010-08-05 |
20100196943 | IDENTIFICATION AND CHARACTERIZATION OF RACEMASES, DEFINITION OF PROTEIN SIGNATURES, AND A TEST FOR DETECTING D-AMINO ACID AND FOR SCREENING MOLECULES CAPABLE OF INHIBITING THE ACTIVITY OF RACEMASE, ESPECIALLY PROLINE RACEMASE - This invention relates to the identification and characterization of racemases and definition of protein signatures of these racemases. More particularly, this invention relates to the identification of nucleic acid molecules encoding a peptide consisting of a motif characteristic of the protein signatures, and to the peptides consisting of these motifs and more specifically SEQ ID NOS:1-4. This invention also relates to antibodies specific for the peptides and to immune complexes of these antibodies with the peptides. Further, the invention relates to methods and kits for detecting racemases using the nucleic acid molecules of the invention, as well as the peptides consisting of the motifs and antibodies to these peptides. | 2010-08-05 |
20100196944 | METHOD OF BIOASSAYING YOKUKANSAN - To find out an in-vitro bioassay system capable of ensuring qualities of yokukansan to a higher degree, it is intended to provide a method of bioassaying yokukansan characterized by comprising adding a yokukansan-containing test sample to astroglial cells to be cultivated under the condition of thiamine deficiency, and then determining the pharmacological activity value of yokukansan based on the glutamic acid or neutral red intake level in the cultivated astroglial cells. | 2010-08-05 |
20100196945 | DEVICE AND METHOD FOR DETERMINING THE ERYTHROCYTE SEDIMENTATION RATE IN A BLOOD SAMPLE - The invention discloses a blood analyzing device ( | 2010-08-05 |
20100196946 | DEVICE AND METHOD FOR CONDITIONING BIOLOGICAL CELLS - A conditioning device ( | 2010-08-05 |
20100196947 | Polyelectrolyte Multilayer Films At Liquid-Liquid Interfaces - The present invention is directed to methods for providing a polyelectrolyte multilayer film at a liquid-liquid interface. Such methods include steps of sequentially-depositing layers of cationic and anionic polyelectrolytes at a liquid-liquid interface that is formed between immiscible first and second liquids whereby a polyelectrolyte multilayer film is provided at the liquid-liquid interface. In certain preferred embodiments, the first liquid is an aqueous solution and the second liquid is a liquid crystal. In alternative embodiments, the first liquid is an aqueous solution and the second liquid is an oil. The invention further encompasses polyelectrolyte multilayer films provided by the disclosed methods as well as applications utilizing such materials. | 2010-08-05 |
20100196948 | METHOD OF SCREENING BAFF SUPPRESSOR OR INHIBITOR - A method of screening a novel BAFF suppressor or inhibitor. More specifically speaking, a method which comprises adding a combination of TPA with ionomycin and/or an anti-CD3 antibody to a cultured human cell to thereby induce the production of BAFF by the cell; a method of screening a substance capable of suppressing the expression or activity of BAFF which comprises adding a test substance to a BAFF-production system prepared by adding a combination of TPA with ionomycin and/or an anti-CD3 antibody to a cultured human cell and measuring the expression amount and/or the activity of BAFF in the BAFF-production system; and a BAFF production inducer for a BAFF-producing cell which contains a combination of TPA with ionomycin and/or an anti-CD3 antibody. | 2010-08-05 |
20100196949 | SAMPLE WELL STRIP - A multiple cuvette strip comprises a plurality of wells and a reversible interlocking device. The well strips can be reversibly interlocked to other well strips to form a sample holder system. One embodiment of a well strip comprises a flange and a slot to form a reversible interlocking device. | 2010-08-05 |
20100196950 | AUTOMATED SEED SAMPLER AND METHODS OF SAMPLING, TESTING AND BULKING SEEDS - An automated seed sampler includes a sampling station; a sampler for removing material from a seed in the sampling station; a seed conveyer for conveying the seed from the sampling station to a compartment in a seed tray; and a conveyor for conveying the material removed from the seed to a corresponding compartment in a sample tray. The method of the present invention comprises feeding seeds individually to a sampling station, removing a sample from the seed in the sampling station; conveying the sample to a compartment in a sample tray, and conveying the seed to a corresponding compartment in a seed tray. The samples can be tested, and the seeds can be sorted according to the results of the testing of their corresponding samples. | 2010-08-05 |
20100196951 | Antitoxin Destabilization Technology - Disclosed are methods for purifying toxin proteins which avoid denaturation and renaturation procedures. | 2010-08-05 |
20100196952 | METHOD FOR PREPARATIVE IN VITRO PROTEIN BIOSYNTHESIS - The invention relates to a method for preparative in vitro protein synthesis of an expression product in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined expression product, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, without separating generated substances and without adding consumed synthesis substances within the defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products and/or reaction inhibitors are separated from the solution (and extracted), d) immediately before, after or at the same time as step c) consumed synthesis substances are supplemented, e) steps b), c) and d) are repeated at least once with the reaction solution of step d), and at the last execution of step b) steps c) and d) may be left out. | 2010-08-05 |
20100196953 | CLEAVAGE OF PRECURSORS OF INSULINS BY A VARIANT OF TRYPSIN - The present invention relates to the production of a variant of recombinant trypsin with increased substrate specificity for arginine versus lysine in non-animal host organisms. Moreover, the present invention relates to a variant of recombinant trypsin and their production. Also provided are use of recombinant porcine pancreatic trypsin variants for cleavage of precursors of insulins, and kits containing the variant of trypsin. | 2010-08-05 |
20100196954 | Modified Endoglucanase II and Methods of Use - Described herein is a modified EGII cellulase having the amino acid sequence shown in SEQ ID NO:1, compositions comprising the modified EGII and methods of use. | 2010-08-05 |
20100196955 | Scaleable Manufacturing Process for Cysteine Endoprotease B, Isoform 2 - Methods are provided for the production of gram to kilogram quantities of pro-EP-B2 (proenzyme form of EP-B2) in a lyophilized form. The methods include scalable fermentation, refolding and purification processes, which processes may be combined with lyophilization to yield a stable product. | 2010-08-05 |
20100196956 | NOVEL CONSTITUTIVE STRONG PROMOTER AND USE THEREOF - Disclosed herein are an aldolase gene promoter and the use thereof, more particularly, disclosed are a promoter of aldolase gene derived from | 2010-08-05 |
20100196957 | CLONING AND CHARACTERIZATION OF 5' FLANKING REGIONS OF A HUMAN AGGRECANASE-1 GENE - The present invention relates to an isolated polynucleotide molecule comprising an Agg-1 promoter gene and methods of using the same. The invention also provides a method for producing an expression product encoded by a nucleic acid through use of an Agg-1 promoter. | 2010-08-05 |
20100196958 | Reduced Phosphotransferase System Activity in Bacteria - A method of producing biological products using bacteria with an inactivated ptsHI and wild type err and no added glucose transport activity and which consumes nearly all glucose in the media is described. The ΔptsHI bacteria produce large quantities of recombinant protein without producing significant amounts of acetate. The bacteria grow well on standard LB broth without additional supplementation. | 2010-08-05 |
20100196959 | ALLELES OF THE OPCA GENE FROM CORYNEFORM BACTERIA - The invention relates to mutants and alleles of the opcA gene of coryneform bacteria, which encode variants of the OpcA subunit of glucose 6-phosphate dehydrogenase (EC: 1.1.1.49), and to processes for preparing amino acids, in particular L-lysine and L-tryptophan, by using bacteria which harbor said alleles. | 2010-08-05 |
20100196960 | TRANSPORTER GENES OATP-B, C, D, AND E - Four novel transporter genes were successfully cloned by screening novel transporter genes based on the human OATP transporter gene sequence. These transporters are useful in the development of drugs by taking advantage of the activity of transporting biological substances and various drugs. It was also found that these transporter genes have single nucleotide polymorphisms (SNP). Gene diagnosis based on the polymorphisms (such as SNP) in these transporter genes enables one to judge, for example, the efficacy of a drug therapy. | 2010-08-05 |
20100196961 | VARIABLE TANGENTIAL FLOW FILTRATION - The current invention reports a method for concentrating an immunoglobulin solution by tangential flow filtration wherein the transmembrane pressure and the cross-flow are variable. | 2010-08-05 |
20100196962 | IL-6 RECEPTOR IL-6 DIRECT FUSION PROTEIN - The present invention intends to provide an IL-6R.IL-6 fusion protein and the like in which IL-6R and IL-6 are directly linked without a linker. | 2010-08-05 |
20100196963 | CONDITIONED MEDIUM AND EXTRACELLULAR MATRIX COMPOSITIONS FROM CELLS CULTURED UNDER HYPOXIC CONDITIONS - The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications. | 2010-08-05 |
20100196964 | METHOD FOR THE PRODUCTION OF AN AQUEOUS GLUCOSE SOLUTION - The present invention relates to a process for the production of an aqueous glucose solution from maize or maize kernels. The invention also relates to a glucose solution obtainable by this process, and to its use for the production of organic compounds. The process according to the invention comprises: a) fractionating dry milling of maize kernels, where the maize kernels are separated into a maize-starch-comprising endosperm fraction and a high-oil germ fraction and, if appropriate, a bran fraction; b) enzymatic liquefaction and saccharification of the maize starch in an aqueous suspension of the endosperm fraction, which gives an aqueous glucose solution comprising maize gluten; and c) depletion of the maize gluten and, if appropriate, any bran present from the aqueous glucose solution. | 2010-08-05 |
20100196965 | Acidophilic Enzymes - The present invention relates to enzymes having catalytic activity at a pH below 5.0. The present invention provides hydrolyzing enzymes obtainable from archaeobacteria, in detail to hydrolytic enzymes obtainable from the archaeobacterium | 2010-08-05 |
20100196966 | PLANTS WHICH SYNTHESIZE INCREASED AMOUNTHS OF GLUCOSAMINGLYCANS - The present invention relates to plant cells and plants which synthesize an increased amount of glucosaminoglycans, and to methods for preparing such plants, and also to methods for preparing glucosaminoglycans with the aid of these plant cells or plants. Here, plant cells or genetically modified plants according to the invention have glucosaminoglycan synthase activity and additionally an increased glucosamine 6-phosphate acetyltransferase activity and an increased UDP-N-acetyl-glucosamine pyrophosphorylase activity compared to wild-type plant cells or wild-type plants. The present invention furthermore relates to compositions comprising plant cells having an increased glucosaminoglycan synthesis. | 2010-08-05 |
20100196967 | FRACTIONATION OF A LIGNOCELLULOSIC MATERIAL - A method for fractionating a lignocellulosic material, the method comprising; contacting ( | 2010-08-05 |
20100196968 | Polypeptides having beta-glucosidase activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having beta-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. | 2010-08-05 |
20100196969 | METHOD FOR OBTAINING ENERGY-GENERATING COMPOUNDS BY MEANS OF ELECTROMAGNETIC ENERGY - The present invention relates to a process for obtaining energetic compounds by means of electromagnetic energy produced by sunlight or artificial light by means of using phytoplankton cultures. | 2010-08-05 |
20100196970 | Butynol I esterase - The invention relates to novel proteins from | 2010-08-05 |
20100196971 | SEQUESTRATION OF COMPOUNDS FROM MICROORGANISMS - The invention provides novel mesoporous particles and methods for the selective sequestration of organic compounds from microorganisms, such as from various genera and species of algae that produce important organic compounds. The organic compounds can be selectively sequestered, for example, in favor of biodiesel impurities such as sterols and chlorophyll, to provide substantially pure free fatty acids. The free fatty acids can then be esterified to provide pure biodiesel. | 2010-08-05 |
20100196972 | PRODUCING ENERGY FROM BIOLOGICAL MATERIAL - Described are methods and systems for producing synthesis gas. In some embodiments, the method includes microbiologically converting biological material to form methane and CO2; and reacting methane and CO2 formed in (a) with water and metal to form synthesis gas. Optionally, the method also includes cutting vegetation; fermenting the vegetation to form biogas comprising methane and CO2; and reacting the biogas with water and metal to form synthesis gas and metal oxide. In some embodiments of the invention, reacted metal is regenerated from metal oxide produced in the reaction. In some embodiments the regeneration comprises reacting the oxide in a bath of boiling zinc. | 2010-08-05 |
20100196973 | METHODS FOR THE SYNTHESIS OF FATTY DIACIDS BY THE METATHESIS OF UNSATURATED DIACIDS OBTAINED BY FERMENTATION OF NATURAL FATTY ACIDS - The invention relates to a method for the synthesis of diacids of general formula ROOC—(CH | 2010-08-05 |
20100196974 | Chemically Inducible Expression of Biosynthetic Pathways - Methods and constructs for the introduction of multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway are provided. In one embodiment, the constructs contain two or more enzyme-encoding genes, each under the control of an inducible promoter and each with a polyadenylation signal. The constructs are used to produce transgenic plants, in which the expression of the enzymes are increased when a chemical inducing agent is applied, and a biosynthetic product of the series of enzymes encoded by the transgenes is produced. Constructs may be used which contain two or more enzyme-encoding genes under the control of one or more promoters activated by activator molecules or complexes expressed from a transgene or transgenes, which are themselves under the control of one or more inducible promoters and switched on following the external application of a chemical. The transgene or transgenes expressing the activator molecules or complexes may be included in the same construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. Alternatively, the transgene or transgenes expressing the activator molecules or complexes may be on a different construct from the construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. The activator molecule can be expressed using a constitutive promoter in an inactive form which is converted to the active form following application of the chemical inducing agent. | 2010-08-05 |
20100196975 | SEQUESTRATION OF FORMALDEHYDE TO STABILIZE NITRILASE SPECIFIC ACTIVITY WHEN CONVERTING GLYCOLONITRILE TO GLYCOLIC ACID - A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst. | 2010-08-05 |