30th week of 2012 patent applcation highlights part 41 |
Patent application number | Title | Published |
20120189991 | FREEFORM MATHEMATICAL PARSING AND GRADING METHOD AND SYSTEM - Described herein are embodiments of systems, methods, apparatus and computer program products that accept student input and use a computer to process its mathematical meaning. The input can then be algorithmically tested for certain mathematical criteria. Those test results then allow the system to output immediate assessment, grades, and feedback. | 2012-07-26 |
20120189992 | Apparatus And Method For Establishing Standards For Visual Residue Limits - Embodiments of the present invention are directed to apparatus and methods for establishing standards for visual residue limits, training individuals to visually observe and recognize surfaces exceeding or complying with residue limits, and certifying individuals for such detection limits The invention features a kit comprising an assembly of coupons with predetermined loads. | 2012-07-26 |
20120189993 | VIRTUAL WELDING SYSTEM - This written description uses examples to disclose the invention, including the best mode, and also to enable one of ordinary skill in the art to practice the invention, including making and using any devices or systems and performing any incorporated methods. The patentable scope of the invention is defined by the claims, and may include other examples that occur to those skilled in the art. Such other examples are intended to be within the scope of the claims if they have structural elements that are not different from the literal language of the claims, or if they include equivalent structural elements with insubstantial differences from the literal language of the claims. | 2012-07-26 |
20120189994 | Methods and Apparatuses To Enhance Cognitive Functioning And Its Manifestation Into Physical Form and Translation Into Useful Information - The present invention provides a method and apparatus for enhancing cognitive functioning and its manifestation into physical form and translation into useful information for improving functioning in human experience, for example, business, academic or personal endeavors. | 2012-07-26 |
20120189995 | Dream Little Dreamer (DLD) Systems and Methods - The present invention referred to as “Dream Little Dreamer” (DLD) provides an online social and educational environment focusing on positive human characteristics that influence children and on building self confidence. Dream Little Dreamer provides age appropriate learning materials geared towards preschool children to young adults (also referred to herein as “children”). Children are rewarded for completion and accuracy of educational and/or social activities with points that may be used to accomplish goals of building a dream world. Additional embodiments include online and live talent contests that include opportunities to learn online from other children and professional. | 2012-07-26 |
20120189996 | METHOD AND SYSTEM FOR QUANTIFYING TECHNICAL SKILL - A system and method for quantifying clinical skill of a user, comprising: collecting data relating to a surgical task done by a user using a surgical device; comparing the data for the surgical task to other data for another similar surgical task; quantifying the clinical skill of the user based on the comparing of the data for the surgical task to the other data for the other similar surgical task; outputting the clinical skill of the user. | 2012-07-26 |
20120189997 | FLEXIBLE AND RIGID ENDOSCOPIC TRAINING DEVICE (FRED) - An anatomically realistic model for training minimally invasive procedures. The model includes an outer shell resembling the appropriate animal or human being with openings for accessing the interior cavity of the shell. Modules within the interior cavity are operably connected to the openings to permit external access to the modules. The modules comprise different mechanical components configured to represent an organ or anatomical feature. Spacers within the interior cavity provide structure and maintain placement of the modules and sufficiently realistic tactile feedback during an endoscopic procedure. | 2012-07-26 |
20120189998 | TUMOR ABLATION TRAINING SYSTEM - A training system and method includes a subject phantom ( | 2012-07-26 |
20120189999 | SYSTEM AND METHOD FOR USING OPTICAL CHARACTER RECOGNITION TO EVALUATE STUDENT WORKSHEETS - A system and method for evaluating worksheets are provided. The method includes accessing by a processor a digitally stored image of a worksheet that was administered to a student, wherein the worksheet includes the presentation of at least one problem for the student to respond to and each problem presentation includes an answer region that the student manually, mechanically, or electronically marked with an answer to the problem using a variety of alphanumeric characters. The method further includes generating expected answer data that provides an expected answer for each answer region; locating each answer region in the image; extracting from each located answer region the student's marks; processing the student's marks extracted from each answer region with character recognition processing; and storing the processed marks as student answer data. The student answer data is evaluated by comparing the student answer data with the expected answer data and generating corresponding evaluation data. | 2012-07-26 |
20120190000 | Learning Asset Technology Integration Support Tool - A computer implemented process for making a pedagogically informed selection of a learning technology for a learning objective. Predetermined learning outcomes taxonomy level(s) for learning objective(s) are presented. Predetermined instructional strategy(ies) aligned with a selected learning outcomes taxonomy level chosen from the Predetermined learning outcomes taxonomy level(s) are presented. Predetermined learning technolog(ies) chosen from the predetermined instructional strategy(ies) are presented. | 2012-07-26 |
20120190001 | AUTOMATED COGNITIVE TESTING METHODS AND APPLICATIONS THEREFOR - An automated cognitive test that provides screening, scoring and real time results based on comparing the test results to updated normed results. The test can be self administered. The questions are randomly chosen from a selected database. Customizable reports may be generated from the test results and used in industry. | 2012-07-26 |
20120190002 | METHOD SYSTEM AND COMPUTER-READABLE MEDIA FOR WEB BASED TRAINING ON AN INSTRUMENT OR PIECE OF EQUIPMENT - Disclosed are methods and systems adapted to provide a single-source, e-training platform for generating an e-training plan and providing e-training courses directed at various competencies in the operation, maintenance, calibration, control testing, and/or troubleshooting of an instrument or piece of equipment. The system includes an e- training module having a content library sub-module of e- training courses, a trainee planner, and a validation sub- module adapted to validate a completion of a practical portion. An administrator module adapted to generate an invite to a trainee, and allow generation of an e-learning plan for a trainee. Computer-readable medium containing an executable version of the methods are provided, as are other aspects. | 2012-07-26 |
20120190003 | ORGAN ARREST, PROTECTION, PRESERVATION AND RECOVERY - The present invention relates to a composition for controlling viability of a tissue including a potassium channel opener or adenosine receptor agonist, a compound for inducing local anaesthesia and a compound for reducing the uptake of water by a cell in the tissue. The present invention also relates to the use of the composition according to the invention for controlling viability of a tissue. | 2012-07-26 |
20120190004 | METHODS AND COMPOSITIONS FOR THE CRYOPRESERVATION OF DUCKWEED - The present invention describes methods for the cryopreservation of duckweed plants and duckweed plant tissues. The methods comprise freezing a dehydrated duckweed frond colony to a cryopreservative temperature to obtain a frozen frond colony comprising at least one cryopreserved duckweed plant or a cryopreserved duckweed plant tissue. The method can comprise a dehydration step whereby a duckweed frond colony is dehydrated, and in some embodiments, can further comprise a dormancy-induction step prior to or during the dehydration step. The method further can further comprise a recovery step, wherein the frozen frond colony is thawed and a viable duckweed plant or duckweed plant tissue is recovered. Cryopreserved duckweed plants and duckweed plant tissues, and viable duckweed plants and duckweed tissues recovered therefrom are also provided. In some embodiments, the duckweed frond colony, duckweed plant, and duckweed tissue comprise a heterologous polynucleotide of interest, which can encode a heterologous polypeptide of interest. | 2012-07-26 |
20120190005 | METHOD FOR A RATIONAL CELL CULTURING PROCESS - Biopharmaceutical process development with recombinant protein producing mammalian cells has realized a tremendous increase in both productivity and product yields in the past years. These achievements can be mainly attributed to the advancements in cell line development, media, and process optimization. Only recently, genome-scale technologies enable a system-level analysis to elucidate the complex biomolecular basis of protein production in mammalian cells promising an increased process understanding and the deduction of knowledge-based approaches for further process optimization. The present invention describes a method for a rational cell culturing process using such a knowledge-based approach. | 2012-07-26 |
20120190006 | OPTOELECTRONIC DETECTION SYSTEM - The invention relates to optoelectronic systems for detecting one or more target particles. The system includes a reaction chamber, a specimen collector, an optical detector, and a reservoir containing cells, each of the cells having receptors which are present on the surface of each cell and are specific for the target particle to be detected, where binding of the target particle to the receptors directly or indirectly activates a reporter molecule, thereby producing a measurable optical signal. | 2012-07-26 |
20120190007 | METHOD AND SYSTEM FOR MONITORING AND RECORDING A VIRAL INFECTION PROCESS AND THAT FOR SCREENING VACCINES - The present invention relates to a method and system for monitoring and recording a viral infection process and that for screening vaccines, which is characterized by providing a microcantilever detection device, which comprises a microcantilever comprising a contact area having an macromolecular material attached thereon; allowing host cell to be attached to the macromolecular material; allowing a sample containing a test virus or vaccine to be loaded into the contact area to make the test virus or vaccine to contact the host cells attached to the macromolecular material so as to produce a deflection of the microcantilever; and recording the deflection in a time course manner so as to obtain a deflection profile that can used as a basis for determining the viral infection process or the potential efficacy of the vaccines. | 2012-07-26 |
20120190008 | GENERIC PCR - The present invention provides a method for the amplification of at least a first and a second target nucleic acid that may be present in a fluid sample. The invention further provides a kit and an analytical system for carrying out said amplification. | 2012-07-26 |
20120190009 | Viral Modulators and Processes Thereof - A viral modulator and process thereof. A method may include contacting one or more viral modulators to one or more biological systems. A biological system may be configured to be infected by one or more virus. A virus may include an HIV virus, a VEEV virus and/or the like. A viral modulator may include a viral inhibitor and/or a viral activator. | 2012-07-26 |
20120190010 | Generic Matrix for Control Nucleic Acids - The present invention belongs to the field of in-vitro diagnostics. Within this field, it particularly concerns the amplification of at least a first target nucleic acid that may be present in at least one fluid sample using an internal control nucleic acid for qualitative and/or quantitative purposes and at least one external control nucleic acid in an aqueous buffer. It further provides an analytical system comprising an internal control nucleic acid for qualitative and/or quantitative purposes and at least one external control nucleic acid in an aqueous buffer. | 2012-07-26 |
20120190011 | COMPOSITIONS AND METHODS FOR MAMMALIAN GENETICS AND USES THEREOF - The invention provides compositions and methods for performing mammalian cell genetics, e.g., genetic screens, using near-haploid cells. The invention further provides genes and gene products isolated using the inventive methods and methods of use thereof. | 2012-07-26 |
20120190012 | COMPOSITIONS AND METHODS FOR DNA SEQUENCING - The invention provides compositions and methods useful in DNA sequencing. In exemplary embodiments, a detectable label such as a luminescent macrocycle is used. | 2012-07-26 |
20120190013 | HYDROLASE ENZYME SUBSTRATES AND USES THEREOF - The present invention provides novel methods for determining the presence or amount of a hydrolytic enzyme in a sample, based on novel substrates for the enzymes, and also provides compositions and methods that provide highly sensitive assay methods for such hydrolytic enzymes. | 2012-07-26 |
20120190014 | PHAGE PHI 29 DNA POLYMERASE CHIMERA - A DNA polymerase chimera comprising an amino-terminal (N-terminal) region encoding a φ29 type DNA polymerase and a carboxyl-terminal (C-terminal) region comprising at least one HhH domain which are bound by means of a connecting amino acid sequence is disclosed along with and the use thereof for replicating, amplifying or sequencing a template DNA. Also disclosed is a method for replicating, amplifying or sequencing a deoxyribonucleic acid with said DNA polymerase chimera and kits for carrying out said methods. | 2012-07-26 |
20120190015 | METHOD FOR DETERMINING THE PRESENCE AND CONCENTRATION OF ANALYTES USING A NUCLEIC ACID LIGAND AND RARE EARTH ELEMENTS - The present invention relates to methods and an apparatus for determining the presence and concentration of an analyte in a sample and the binding of the analyte to a nucleic acid ligand that include measuring the fluorescence emitted by a rare earth element, i.e., terbium, in the presence of the analyte and the nucleic acid ligand. Specific embodiments include the use of terbium and nucleic acid ligands that specifically bind the mycotoxin ochratoxin. A, to detect and quantify ochratoxin A in, for example, food samples such as grain, wine, or beer. The detection of thrombin using terbium and a thrombin-specific nucleic acid ligand is also disclosed. The present invention also relates to a composition comprising a rare earth element as a cation that facilitates the binding of an analyte to a nucleic acid ligand of the analyte. | 2012-07-26 |
20120190016 | COMPOSITIONS FOR USE IN IDENTIFICATION OF SALMONELLA - The present invention relates generally to identification of | 2012-07-26 |
20120190017 | POLYMORPHISMS IN THE HUMAN GENE FOR THE MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN 1 (MRP-1) AND THEIR USE IN DIAGNOSTIC AND THERAPEUTIC APPLICATIONS - The present invention relates to a polymorphic MRP-1 polynucleotide, genes or vectors comprising the polynucleotides and a host cell genetically engineered with the polynucleotide or gene. Also provided are methods for producing molecular variant polypeptides, cells capable of expressing a molecular variant polypeptide and to a polypeptide encoded by the polynucleotide or the gene or obtainable by the method or cells produced herein. Also provided is an antibody to the polypeptide, a transgenic animal, and to a solid support comprising one or a plurality of the provided polynucleotides, genes, vectors, polypeptides, antibodies or host cells. Furthermore, methods of identifying a polymorphism, identifying and obtaining a pro-drug or drug or an inhibitor are also provided. In addition, the invention relates to methods for producing of a pharmaceutical composition, diagnosing a disease and, detection of the polynucleotide. Furthermore, provided herein are uses of the polynucleotides, genes, vectors, polypeptides or antibodies herein. | 2012-07-26 |
20120190018 | ENHANCED RISK PROBABILITIES USING BIOMOLECULE ESTIMATIONS - The present invention provides processes for determining more accurate risk probabilities for medical conditions. The risk probabilities of the presence or absence of a medical condition are calculated using frequency data from selected biomolecules and biomolecule source contribution of at least one source in a mixed sample. | 2012-07-26 |
20120190019 | CONCATAMERIC LIGATION PRODUCTS: COMPOSITIONS METHODS AND KITS FOR SAME - The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures. | 2012-07-26 |
20120190020 | DETECTION OF GENETIC ABNORMALITIES - The present invention provides assay systems and related methods for determining genetic abnormalities in mixed samples comprising cell free DNA from both normal and putative genetically atypical cells. Exemplary mixed samples for analysis using the assay systems of the invention include samples comprising both maternal and fetal cell free DNA and samples that contain DNA from normal cells and circulating cancerous cells. | 2012-07-26 |
20120190021 | DETECTION OF GENETIC ABNORMALITIES - The present invention provides assay systems and related methods for determining genetic abnormalities in mixed samples comprising cell free DNA from both normal and putative genetically atypical cells. Exemplary mixed samples for analysis using the assay systems of the invention include samples comprising both maternal and fetal cell free DNA and samples that contain DNA from normal cells and circulating cancerous cells. | 2012-07-26 |
20120190022 | METHOD FOR DETERMINATION OF THE LENGTH OF THE G-TAIL SEQUENCE AND KIT FOR THE METHOD - A method of measuring the length of a G tail sequence, characterized by hybridizing the G tail of an nondenatured chromosomal DNA in a sample with a labeled DNA probe having a sequence complementary to the telomere repeat sequence, measuring chemiluminescence from the hybridized DNA probe, and determining the length of the G tail sequence from the measured value, and a kit used for use in such a method. | 2012-07-26 |
20120190023 | METHODS FOR DISTINGUISHING BETWEEN NATURAL AND ARTIFICIAL DNA SAMPLES - The present invention provides methods for distinguishing between natural and artificial DNA in samples containing nucleic acid molecules. In addition, the present invention provides methods for verifying that DNA profiles obtained from samples represent natural DNA. In various embodiments, the methods employ an array of nucleic acid based procedures for verifying that a DNA sample originates from a natural source. The invention further provides kits for verifying that a DNA sample originates from a natural source employing the methods and reagents described in the disclosure. | 2012-07-26 |
20120190024 | METHOD FOR DETERMINING PRESENCE OR ABSENCE OF EPITHELIAL CANCER-ORIGIN CELL IN BIOLOGICAL SAMPLE, AND MOLECULAR MARKER AND KIT THEREFOR - The present invention provides a method for determination of presence or absence of an epithelial cancer-derived cell in a biological sample obtained from a subject comprising the steps of: extracting DNA from the biological sample, analyzing methylation status of a CpG site located in at least one region represented by base sequences SEQ ID NOs: 1, 2, 3 and 4 in the DNA obtained from the step of extracting, and determining presence or absence of the epithelial cancer-derived cell in the biological sample based on an analysis result obtained from the step of analyzing. | 2012-07-26 |
20120190025 | ENTEROCOCCUS AND FECAL BACTEROIDES FOR RAPID WATER QUALITY ASSESSMENT - The present invention is drawn to methods and compositions for the rapid assessment of fecal indicator bacteria in a sample. Provided herein are novel primer and probe compositions for use in detecting the presence of these organisms in a sample, particularly using quantitative PCR methods. Provided herein are novel oligonucleotide primers and probes, including the primers set forth in SEQ ID NOS: 1-4, 7 and 8, the novel oligonucleotide probe sequences set forth in SEQ ID NOS:5, 6, and 9, and methods for using these primers and probes for the detection and/or quantification of fecal indicator bacteria, particularly | 2012-07-26 |
20120190026 | METHOD OF NORMALIZED QUANTIFICATION OF RNA - The present invention is related to normalized quantification of RNAs and to the normalization of quantities of RNAs in samples, e.g. mixtures of RNAs. The present invention relates to method for the normalization of the quantity of a RNA to be quantified in a sample to the total quantity of RNA in the sample; or to the total quantity of a specific class of RNA in the sample. | 2012-07-26 |
20120190027 | LIGATION-BASED METHOD OF NORMALIZED QUANTIFICATION OF NUCLEIC ACIDS - The present invention is related to normalized quantification of nucleic acids and to the normalization of quantities of nucleic acids in samples, e.g. mixtures of nucleic acids. The present invention relates to method for the normalization of the quantity of a nucleic acid to be quantified in a sample to the total quantity of nucleic acid in the sample; or to the total quantity of a specific class of nucleic acid in the sample. | 2012-07-26 |
20120190028 | NUCLEIC ACIDS AND METHODS FOR THE DETECTION OF ENTEROBACTER SAKAZAKII (CRONOBACTER SPP.) - Provided are detection means and method specific for the genus | 2012-07-26 |
20120190029 | METHODS FOR DETECTING DNA ORIGINATING FROM DIFFERENT INDIVIDUALS - In a first aspect, the present invention features methods for differentiating DNA species originating from different individuals in a biological sample. These methods may be used to differentiate or detect fetal DNA in a maternal sample or to differentiate DNA of an organ donor from DNA of an organ recipient. In preferred embodiments, the DNA species are differentiated by observing epigenetic differences in the DNA species such as differences in DNA methylation. In a second aspect, the present invention features methods of detecting genetic abnormalities in a fetus by detecting fetal DNA in a biological sample obtained from a mother. In a third aspect, the present invention features methods for differentiating DNA species originating from an organ donor from those of an organ recipient. In a fourth aspect, the present invention features kits for differentiating DNA species originating from different individuals in a biological sample. | 2012-07-26 |
20120190030 | Detection of Target Nucleic Acid Sequences by Cyclic Exonucleolytic Reactions - The present invention relates to the detection of a target nucleic acid sequence by a cyclic exonucleolytic reaction. The present method enabling to generate signals by probe digestion with no help of primers and to amplify signals with no help of simultaneous target amplification reactions may enable to detect multiple target sequences without any problems accounted in the conventional real-time PCR methods such as false positive signals and difficulties in oligonucleotides (primer and probe) selection and reaction condition optimization. | 2012-07-26 |
20120190031 | MARKER FOR DIAGNOSIS OF ACTIVE MULTIPLE SCLEROSIS - The invention provides an improved method for monitoring the course of the MS disease and to predict, diagnose or prognosticate whether a subject is in an active or non-active period of MS. The method is based on determining the ratio of the expression levels of two cytokines, measured before and after stimulation of the subject by an immunomodulator. | 2012-07-26 |
20120190032 | DROPLET GENERATION FOR DROPLET-BASED ASSAYS - A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed. | 2012-07-26 |
20120190033 | DROPLET TRANSPORT SYSTEM FOR DETECTION - System, including methods and apparatus, for transporting droplets from a tip to an examination site for detection. | 2012-07-26 |
20120190034 | OPTICAL FIBER MEASUREMENT DEVICE AND MEASUREMENT METHOD USING SAME - Disclosed is a highly reliable optical fiber measurement device and measurement method having a simple and compact structure. The device includes a planar liquid holder having a plurality of liquid holding portions arranged along a flat face; a plurality of light receiving optical fibers for transmitting fluorescence generated in the liquid holding portions; a plurality of light emitting optical fibers for transmitting excitation light into the liquid holding portions; a measurement head capable of being positioned in the each liquid holding portion while supporting a plurality of measurement ends having a bundle of one light receiving end of the light receiving optical fibers and one light emitting end of light emitting optical fibers; a light reception selecting element that, by sequentially selecting one by one from plural the light receiving optical fibers and sequentially selecting one by one from plural kinds of wavelength or wavelength bands, sequentially guides the light of the selected wavelength or wavelength band of the fluorescence received by the selected light receiving optical fibers to one photoelectric element; and a photoelectric element for sequentially conducting photoelectric conversion on the guided fluorescence. | 2012-07-26 |
20120190035 | Detection Of Disease Related Genes - The invention is directed to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives, or substances recognized by the protein synthesizing machinery. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system. The invention also comprises proteins prepared using misaminoacylated tRNAs which can be utilized in pharmaceutical compositions for the treatment of diseases and disorders in humans and other maninials, and kits which may be used for the detection of diseases and disorders. | 2012-07-26 |
20120190036 | CLINICAL METHOD FOR GENOTYPING LARGE GENES FOR MUTATIONS THAT POTENTIALLY CAUSE DISEASE - A method of determining polymorphisms within a large gene comprising the steps of: (a) making a Whole-Genome Amplification (WGA) to obtain sufficient amounts of genetic templates for DNA analysis; (b) enriching the WGA sample with nested primers designed for the large gene; (c) using the enriched WGA sample for high resolution melt (HRM); and (d) detecting differential melt profiles during the transition from double strand to single strand with an increase in temperature wherein sequence point mutations within the gene affects the thermal stability and gives a different melt profile from the normal non-mutated gene sequence, and kits to carry out detection of the same. The method may further comprise the step of spiking the DNA being screened using DNA from a phenotypically normal individual in order to induce synthetic heterozygosity. The method in (d) may also work directly on genomic samples without WGA step if sufficient DNA material is present. | 2012-07-26 |
20120190037 | Device and Method for Isolating Biological or Chemical Targets - The present invention relates to a device for collecting and concentrating biological or chemical targets with a view to detecting same, wherein the device comprises a reaction chamber ( | 2012-07-26 |
20120190038 | LovD MUTANTS EXHIBITING IMPROVED PROPERTIES TOWARDS SIMVASTATIN SYNTHESIS - The invention disclosed herein relates to methods and materials for producing simvastatin and related compounds such as huvastatin. In particular, the disclosure teaches that variants of the LovD acyltransferase polypeptide can be engineered to exhibit properties that facilitate their use in the production of simvastatin and/or huvastatin. The materials and processes disclosed herein are designed so that fermentation facilities currently producing lovastatin can be converted to producing simvastatin and related compounds with minimal modifications. | 2012-07-26 |
20120190039 | RAF DIMERS AND USES THEREOF - Disclosed herein are mutated RAF and KSR nucleic acids and polypeptides. Also disclosed are methods of using the mutated RAF and KSR to inhibit the dimerization of RAF/RAF and RAF/KSR. Also disclosed are methods of using the mutated RAF and KSR to screen for inhibitors of dimerization. | 2012-07-26 |
20120190040 | CELL CONCENTRATION, CAPTURE AND LYSIS DEVICES AND METHODS OF USE THEREOF - The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non-Faradic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force. | 2012-07-26 |
20120190041 | METHODS, IMMUNOASSAYS AND DEVICES FOR DETECTION OF ANTI-LIPOIDAL ANTIBODIES - Compositions, methods and devices for the detection of anti-lipoidal antibodies and the diagnosis of disease, for example, syphilis, are described. In particular, a method for immobilizing a lipoidal antigen, comprising cardiolipin, lecithin, and cholesterol, on a solid support (such as a nitrocellulose membrane) is described. The ability to immobilize a lipoidal antigen on a membrane satisfies a long-felt need for membrane-based assay for the detection of anti-lipoidal antibodies. Also described are immunoassay devices for concurrently performing treponemal and non-treponemal tests for syphilis. | 2012-07-26 |
20120190042 | PROTEIN BIOMARKERS AND THERAPEUTIC TARGETS FOR OSTEOARTHRITIS - The present invention relates to the identification and use of protein expression profiles with clinical relevance to osteoarthritis. In particular, the invention provides the identity of marker proteins whose expressions are correlated with OA, and/or OA progression. Methods and kits are described for using these protein expression profiles in the study and/or diagnosis of OA, in the determination of the degree of advancement of OA, and in the selection and/or monitoring of treatment regimens. The invention also relates to the screening of drugs that modulate expression of these proteins or nucleic acid molecules encoding these proteins, in particular for the development of disease-modifying OA agents. | 2012-07-26 |
20120190043 | ENZYMATIC SUBSTRATES FOR MULTIPLE DETECTION SYSTEMS - An inventive substrate is provided which includes a substrate compound of formula A-B | 2012-07-26 |
20120190044 | METHODS AND COMPOSITIONS FOR DIAGNOSIS AND PROGNOSIS OF RENAL INJURY AND RENAL FAILURE - The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using a plurality of assays, one or more of which is configured to detect a kidney injury marker selected from the group consisting of metalloproteinase inhibitor 2, soluble oxidized low-density lipoprotein receptor 1, interleukin-2, von Willebrand factor, granulocyte-macrophage colony-stimulating factor, tumor necrosis factor receptor superfamily member 11B, neutrophil elastase, interleukin-1 beta, heart-type fatty acid-binding protein, beta-2-glycoprotein 1, soluble CD40 ligand, coagulation factor VII, C—C motif chemokine 2, IgM, CA 19-9, IL-10, TNF-α, and myoglobin as diagnostic and prognostic biomarkers in renal injuries. | 2012-07-26 |
20120190045 | Peptide Inhibitors of ABL Kinases - Provided are purified compounds comprising SEQ ID NO:1, where the tyrosine at residue ( | 2012-07-26 |
20120190046 | DEK as a urine based biomarker for bladder cancer - The present invention is directed to a method of detecting DEK protein in a urine sample. Methods and compositions are provided herein for detecting and diagnosing bladder cancer by chemical-induced precipitation of urine proteins, followed by filtration-induced concentration and Western blot analysis to specifically detect DEK protein. The present method permits specific detection of DEK protein in urine as a biomarker for bladder cancer in humans. | 2012-07-26 |
20120190047 | Levetiracetam Immunoassays - Methods, compositions and kits are disclosed directed at levetiracetam derivatives, immunogens, signal generating moieties, antibodies that bind levetiracetam and immunoassays for detection of levetiracetam. | 2012-07-26 |
20120190048 | METHOD FOR DETERMINING THE BINDING OF A GIVEN COMPOUND TO A MEMBRANE RECEPTOR - The invention relates to a method for determining whether a test compound binds preferentially to a membrane receptor R1 or to a membrane receptor R2, these receptors being known to be expressed on the surface of the cells in monomeric, homodimeric or heterodimeric form. This method is applied using one or two FRET partner pairs. | 2012-07-26 |
20120190049 | GLYPICAN-3 TARGETING OF LIVER CANCER CELLS USING MULTIFUNCTIONAL NANOPARTICLES - Method for labeling and detecting liver cancer cells using an anti-glycipan-3 antibody as a targeting agent and a magnetic nanoparticle as an imaging agent. | 2012-07-26 |
20120190050 | CANCER BIOMARKER AND THE USE THEREOF - Gastric cancer is one of the leading causes of cancer-related death worldwide. To date no specific marker is available for gastric cancer screening. The expression profile of 37 proteins was identified to be consistently different between the plasma of normal and gastric cancer subjects. The expression of complement component C9 protein was validated to be significantly higher in the plasma of gastric cancer compared to normal subjects. This was independent of the gastritis and | 2012-07-26 |
20120190051 | METHODS AND COMPOSITIONS FOR DIAGNOSIS AND PROGNOSIS OF RENAL INJURY AND RENAL FAILURE - Disclosed are methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, disclosed are assays that detect one or more markers selected from the group consisting of Prostatic acid phosphatase, Lactotransfenin, Soluble erythropoietin receptor, Von Willebrand factor, Soluble endothelial protein C receptor, and Beta-2-glycoprotein 1 as diagnostic and prognostic biomarkers in renal injuries. | 2012-07-26 |
20120190052 | Method of determining factor XIII by means of NAD(P)H-analogues - The invention is in the field of in-vitro diagnostics and relates to a photometric method for determining the blood-clotting factor XIII (factor XIII, F XIII) with the aid of NAD(P)H analogues, and a test kit for carrying out the method. The invention is in the field of in-vitro diagnostics and relates to a photometric method for determining the blood-clotting factor XIII (factor XIII, F XIII) with the aid of NAD(P)H analogues, and a test kit for carrying out the method. | 2012-07-26 |
20120190053 | Method of determining factor XIII with the aid of plasma-based reference material - The present invention is in the field of in-vitro diagnostics and relates to a method of determining the blood-clotting factor XIII (factor XIII, F XIII) with the aid of plasma-based reference material, and a test kit for carrying out the method. | 2012-07-26 |
20120190054 | Method of Analyzing Cellulose Decay in Cellulosic Material Hydrolysis - Enzymes and/or polypeptides and/or mixtures of interest are evaluated during hydrolysis of cellulosic material by the use of indicator constituents such as fluorescent agents, resulting in efficient high-throughput analysis of enzymes and/or polypeptides. A high-throughput assay for the analysis of inter alia, pretreated corn stover (PCS) hydrolysis is also disclosed. | 2012-07-26 |
20120190055 | MOLECULE BIOMARKERS OF AUTISM - This invention provides methods and biomarkers for diagnosing autism by identifying cellular metabolites differentially produced in autistic patient samples versus non-autistic controls. Methods for identifying a unique profile of metabolites present of secreted in brain tissue, cerebrospinal fluid, plasma, or biofluids of autistic samples are described herein. The individual metabolites or a pattern of secreted metabolites provide metabolic signatures of autism, which can be used to provide a diagnosis thereof. | 2012-07-26 |
20120190056 | Encoded Microparticles - The present invention relates to an encoded microparticle for labeling an isolated cell or an isolated embryo characterized in that it is made of a biocompatible material and its external shape comprises a code by which it can be identified. The use of an encoded microparticle for labeling and/or tracking isolated biological material. A method of tracking an encoded microparticle in or attached to an isolated cell or embryo using an optical microscope, preferably an inverted optical microscope with an objective substantially between 20×-100×. | 2012-07-26 |
20120190057 | Method for the qualitative and/or quantitative analysis of tumour cells - A method for qualitative and/or quantitative analysis of tumor cells, including the following steps: a) generating a dye complex from an aminocoumarin and cyclodextrin; b) mixing the tumor cells with the dye complex prepared in step a); c) incubating the cells with the dye complex prepared in step a); d) analyzing the tumor cells by means of fluorescence microscopy and/or fluorescence spectrometric analysis. | 2012-07-26 |
20120190058 | METHOD FOR EVALUATING CELL POPULATIONS - The invention describes specific sialylated structures present on human stem cells and cell populations derived thereof. The invention is especially directed to methods to control the status of stem cells by observing changes in sialylation of the cells; and control of potential contaminations of biological materials; and reagents and methods used in connection with the cells in order to avoid alterations of the cell glycosylation by contaminating materials. The invention is further directed to novel stem cells, the glycosylation of which has been specifically altered. | 2012-07-26 |
20120190059 | METHODS FOR OBTAINING HEPATOCYTES, HEPATIC ENDODERM CELLS AND HEPATIC PROGENITOR CELLS BY INDUCED DIFFERENTIATION - The present invention discloses a method for inducing the differentiation of embryonic stem cells (ESC) or induced pluripotent stem cells (iPS cells) into hepatocytes, a method for inducing the differentiation of embryonic stem cells or induced pluripotent stem cells into hepatic endoderm cells, and a method for inducing the differentiation of embryonic stem cells (ESC) or induced pluripotent stem cells into hepatic progenitor cells. The present invention also provides the hepatocytes, hepatic endoderm cells and hepatic progenitor cells obtained by above methods, and the uses of these cells. | 2012-07-26 |
20120190060 | MICROFLUIDIC DEVICES AND METHODS FOR MALARIA DETECTION - A device for identifying infection by the malaria parasite includes a microfluidic device having an inlet and an outlet and a diagnostic channel interposed between the inlet and the outlet. The diagnostic channel includes a contact surface and a sample pump configured to pump a RBC-containing sample into the inlet. The contact surface may be at least one of hydrophilic and roughened. Malaria infected RBCs (miRBCs) interact with the contact surface and become immobilized thereon whereas non-infected RBCs continue to flow downstream in the diagnostic channel. | 2012-07-26 |
20120190061 | NOVEL STRATEGY TO REDUCE LACTIC ACID PRODUCTION AND CONTROL PH IN ANIMAL CELL CULTURE - The present disclosure provides a method for culturing cells in exogenous lactic acid. Certain aspects of the present disclosure include the production of recombinant proteins, such as antibodies and fragments thereof. Certain aspects of the present disclosure also relate to methods of controlling lactic acid production, pH stability and osmolality in cell culture. | 2012-07-26 |
20120190062 | PROCESS FOR THE CONVERSION OF BIOMASS TO LIQUID FUELS AND SPECIALTY CHEMICALS - A process is disclosed for converting biomass to fuels and/or valuable chemicals. The process comprises the steps of a) activating biomass to make it more susceptible to conversion; c) partially converting the biomass to a solubilized material; and d) subjecting the unconverted biomass to a second conversion step. The process optionally comprises a step b) of adding a solvent to the activated biomass. In a preferred embodiment the solubilized biomass obtained in step c) is removed before the unconverted biomass is subjected to step d). | 2012-07-26 |
20120190063 | Rhodanobacter Ginsenosidimutans KCTC22231T-Derived Ginsenoside Glycosidase, and Use Thereof - The present invention relates to | 2012-07-26 |
20120190064 | FEEDING BUFFERS, SYSTEMS, AND METHODS FOR IN VITRO SYNTHESIS OF BIOMOLECULES - Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems. | 2012-07-26 |
20120190065 | COMBINATORIAL ENGINEERING - The invention concerns the field of cell culture technology. It concerns production host cell lines with increased expression of ribosomal RNA (rRNA) achieved through introduction of nucleic acids encoding UBF or reducing expression of NoRC proteins, especially of TIP-5. Those cell lines have improved secretion and growth characteristics in comparison to control cell lines. The invention further concerns a method of producing proteins using the cells generated by the described method. | 2012-07-26 |
20120190066 | HOST CELL FOR THE PRODUCTION OF A COMPOUND OF INTEREST - The present invention relates to a recombinant host cell for the production of a compound of interest. The invention further relates to a method for the production of such host cell. The invention further relates to the production of a compound of interest. The invention further relates to isolated polynucleotides and vectors and host cells comprising said polynucleotides. | 2012-07-26 |
20120190067 | Promoter Variants For Expressing Genes In A Fungal Cell - The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NOs: 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances. | 2012-07-26 |
20120190068 | DRAIN DOWN AND RE-FEED OF MICROCARRIER BIOREACTOR - Disclosed is a method of increasing product yield per culture in a population of bound product-secreting cells in a bioreactor, the method comprising: semi-harvesting product by removing a volume of the culture medium with a first-secreted product concentration; re-feeding the bound population of product-secreting cells by adding an amount of a fresh culture medium sufficient to increase the volume of the culture medium to approximately the original volume of the culture medium; agitating the culture medium under sufficient conditions and for a sufficient time period to allow the bound population of product-secreting cells to grow and to release a second-secreted product concentration into the culture medium; and harvesting product. | 2012-07-26 |
20120190069 | GLUTAMINE-AUXOTHROPHIC HUMAN CELLS CAPABLE OF PRODUCING PROTEINS AND CAPABLE OF GROWING IN A GLUTAMINE-FREE MEDIUM - A glutamine-auxotrophic human cell transfected with an exogenous DNA sequence encoding a protein or an exogenous DNA sequence capable of altering the expression of an endogenous gene encoding a protein and an exogenous DNA sequence encoding a glutamine synthetase, wherein these exogenous DNA sequences are located on one or more than one DNA construct, said transfected cell capable of producing said protein and capable of growing in a glutamine-free medium. | 2012-07-26 |
20120190070 | Sp35 Antibodies and Uses Thereof - Endogenous Sp35 is a negative regulator for neuronal survival, axon regeneration, oligodendrocyte differentiation and myelination. Molecules that block endogenous Sp35 function, such anti-Sp35 antibodies can be used as therapeutics for the treatment of neuron and oligodendrocyte dysfunction. The present invention provides antibodies specific for Sp35, and methods of using such antibodies as antagonists of endogenous Sp35 function. The invention further provides specific hybridoma and phage library-derived monoclonal antibodies, nucleic acids encoding these antibodies, and vectors and host cells comprising these antibodies. The invention further provides methods of promoting oligodendrocyte survival and myelination in a vertebrate, comprising administering to a vertebrate in need of such treatment an effective amount of an anti-Sp35 antibody. | 2012-07-26 |
20120190071 | LIPA AND ITS VARIANT USEFUL FOR BIOFUEL PRODUCTION - The present invention relates to LipA and its variant that binds to the glucosyl- and acyl chain- region of its glycoside substrate in plant cell walls useful for biofuel production, wherein presence of a hydrophobic pocket confers extensive hydrophobic interaction of the acyl chain with the rest of the tunnel residues. | 2012-07-26 |
20120190072 | PROTEIN - The present invention relates to a method for preparing a variant lipolytic enzyme comprising expressing in a host organism a nucleotide sequence which has at least 90% identity with a nucleotide sequence encoding a fungal lipolytic enzyme and comprises at least one modification at a position which corresponds in the encoded amino acid sequence to a) the introduction of at least one glycosylation site in the amino acid sequence compared with the original fungal lipolytic enzyme; b) the introduction of at least one amino acid at a surface position and at a location in an external loop distal to the active site of the enzyme which is more hydrophilic; or c) a substitution or insertion at one or more of positions disclosed herein or a deletion at one or more positions disclosed herein. The invention also relates to polypeptide produced by the method and to novel nucleic acids. | 2012-07-26 |
20120190073 | PROCESS FOR LARGE SCALE PRODUCTION OF PLASMID DNA BY E. COLI FERMENTATION - The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a highly productive clonal subtype of a strain of | 2012-07-26 |
20120190074 | THERMOCYCLER AND SAMPLE PORT - The invention relates to continuous flow systems, in particular thermocyclers for the automated and continuous cycling of fluid between a plurality of temperature zones in the amplification of nucleic acids. The invention also relates to an improved sample port for introducing a volume of a liquid sample into a continuous flow system. | 2012-07-26 |
20120190075 | AMYLASE POLYPEPTIDES - This invention relates to polypeptides, more specifically amylase polypeptides and nucleic acids encoding these, and their uses e.g. as non-maltogenic exoamylases in producing food or feed products. | 2012-07-26 |
20120190076 | RECOMBINANT C1 BETA-GLUCOSIDASE FOR PRODUCTION OF SUGARS FROM CELLULOSIC BIOMASS - The invention relates to expression of a recombinant C1 β-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting celiobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 β-glucosidase, operably linked to heterologous promoter, under conditions in which β-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said β-glucosidase to glucose. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel. | 2012-07-26 |
20120190077 | Culture Medium and Methods for Producing Alginate From Stable Mucoid Strains of Pseudomonas Aeruginosa - A specialized culture medium for the promotion of alginate production by stable mucoid | 2012-07-26 |
20120190078 | THREE-DIMENSIONAL BIOPRINTING OF BIOSYNTHETIC CELLULOSE (BC) IMPLANTS AND SCAFFOLDS FOR TISSUE ENGINEERING - A novel BC fermentation technique for controlling 3D shape, thickness and architecture of the entangled cellulose nano-fibril network is presented. The resultant nano-cellulose based structures are useful as biomedical implants and devices, are useful for tissue engineering and regenerative medicine, and for health care products. More particularly, embodiments of the present invention relate to systems and methods for the production and control of 3-D architecture and morphology of nano-cellulose biomaterials produced by bacteria using any biofabrication process, including the novel 3-D Bioprinting processes disclosed. Representative processes according to the invention involve control of the rate of production of biomaterial by bacteria achieved by meticulous control of the addition of fermentation media using a microfluidic system. In exemplary embodiments, the bacteria gradually grew up along the printed alginate structure that had been placed into the culture, incorporating it. After culture, the printed alginate structure was successfully removed revealing porosity where the alginate had been placed. Porosity and interconnectivity of pores in the resultant 3-D architecture can be achieved by porogen introduction using, e.g., ink-jet printer technology. | 2012-07-26 |
20120190079 | METHOD FOR RACEMIZATION OF OPTICALLY ACTIVE ALPHA -AMINO ACIDS - The present invention relates to a method for racemizing an optically active α-amino acid with a viable bacterial cell producing γ-aminobutyric acid aminotransferase or a processed product thereof. According to the present invention, racemic α-amino acids of high quality can be manufactured inexpensively. | 2012-07-26 |
20120190080 | O-PHOSPHOSERINE SULFHYDRYLASE MUTANTS AND METHOD FOR PRODUCTION OF CYSTEINE USING THE SAME - Disclosed is an O-phosphoserine sulfhydrylase (OPSS) mutant which has a | 2012-07-26 |
20120190081 | MICROORGANISM PRODUCING O-PHOSPHOSERINE AND METHOD OF PRODUCING L-CYSTEINE OR DERIVATIVES THEREOF FROM O-PHOSPHOSERINE USING THE SAME - The present invention provides methods for the production of cysteine or derivates thereof by culturing a microorganism having reduced activity of endogenous phosphoserine phosphatase. The O-phosphoserine produced by such an organism can then be reacted with a sulfide in the presence of a sulfydrylase or a microorganism expressing a sulfhydrylase to produce cysteine or a derivative thereof. Microorganisms having the properties noted above are also provided herein. | 2012-07-26 |
20120190082 | MICROORGANISM PRODUCING O-PHOSPHOSERINE AND METHOD OF PRODUCING L-CYSTEINE OR DERIVATIVES THEREOF FROM O-PHOSPHOSERINE USING THE SAME - The present invention provides methods for the production of cysteine or derivates thereof by culturing a microorganism having reduced activity of endogenous phosphoserine phosphatase and enhanced activity of phosphoglycerate dehydrogenase and/or phosphoserine aminotransferase. The O-phosphoserine produced by such an organism can then be reacted with a sulfide in the presence of a sulfydrylase or a microorganism expressing a sulfhydrylase to produce cysteine or a derivative thereof. Microorganisms having the properties noted above are also provided herein. | 2012-07-26 |
20120190083 | MICROORGANISM PRODUCING O-PHOSPHOSERINE AND METHOD OF PRODUCING L-CYSTEINE OR DERIVATIVES THEREOF FROM O-PHOSPHOSERINE USING THE SAME - The present invention provides methods for the production of cysteine or derivates thereof by culturing a microorganism having reduced activity of endogenous phosphoserine phosphatase and the activity of PhnC, PhnD, and PhnE is reduced, and enhanced activity of phosphoglycerate dehydrogenase and/or phosphoserine aminotransferase. The O-phosphoserine produced by such an organism can then be reacted with a sulfide in the presence of a sulfydrylase or a microorganism expressing a sulfhydrylase to produce cysteine or a derivative thereof. Microorganisms having these reduced and enhanced properties noted above are also provided herein. | 2012-07-26 |
20120190084 | Process for the Fermentative Preparation of Sulphur-Containing Amino Acids - Disclosed herein is a process for the fermentative preparation of sulphur-containing amino acids chosen from the group of L-methionine, L-cysteine, L-cystine, L-homocysteine and L-homocystine, comprising the steps: a) provision of a microorganism of the family Enterobacteriaceae or of a microorganism of the family Corynebacteriaceae which has an increased thiosulphate sulphurtransferase activity compared with the particular starting strain; b) fermentation of the microorganism from a) in a medium which contains an inorganic source of sulphur chosen from the group of salt of dithiosulphuric acid or a mixture of a salt of dithiosulphuric acid and a salt of sulphuric acid, a fermentation broth being obtained, and c) concentration of the sulphur-containing amino acid in the fermentation broth from b). | 2012-07-26 |
20120190085 | Transaminase Reactions - The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones. | 2012-07-26 |
20120190086 | KETOREDUCTASE-MEDIATED STEREOSELECTIVE ROUTE TO ALPHA CHLOROALCOHOLS - The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of α chloroalcohols from α chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides. | 2012-07-26 |
20120190087 | PREPARATION OF 1,4-DIAMINOBUTANE - The present invention relates to a novel method for the preparation of 1,4-diaminobutane [DAB]. The method according to the present invention involves at least one biocatalytic step which comprises the biocatalytic production of at least one N-protected precursor of DAB. The present invention also relates to a method for the preparation of DAB involving at least one biocatalytic step, and comprising the steps of a) biocatalytically preparing an N-protected precursor of DAB yielding a—biocatalytic reaction mixture containing the N-protected precursor of DAB, b) recovering the N-protected precursor from the biocatalytic reaction mixture and c) converting the N-protected precursor into DAB. More in particular, the present invention relates to a method for the preparation of DAB, wherein the at least N-protected precursor of DAB is selected from the group consisting of N5-protected ornithine, N-protected DAB, and N-protected 4-aminobutyraldehyde. | 2012-07-26 |
20120190088 | METHOD OF PRODUCING FATTY ACID ALKYL ESTER USING MICROORGANISMS HAVING ABILITY TO PRODUCE OIL - The present invention relates to a method of producing a fatty acid alkyl ester using microorganisms having the ability to produce oil, and more particularly to a method of producing a fatty acid alkyl ester, the method comprising culturing microorganisms having the ability to produce oil, thus accumulating a large amount of oil in the microorganisms, inducing the autolysis of the produced oil in the microorganisms to produce a free fatty acid, and converting the free fatty acid into an alkyl ester. According to the method of the present invention, oil accumulated in microorganisms, such as triacylglycerol that is typical oil produced by microorganisms, can be converted into a fatty acid alkyl ester with high efficiency using a metabolic engineering approach. Thus, the method of the present invention is useful for the industrial production of a fatty acid alkyl ester which has been recently found to be effective as biodiesel. | 2012-07-26 |
20120190089 | ENGINEERED MICROOGRANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS - The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention. | 2012-07-26 |
20120190090 | MICROBIAL CONVERSION OF PLANT BIOMASS TO ADVANCED BIOFUELS - The invention provides consolidated bioprocessing methods and host cells. The host cells are capable of directly converting biomass polymers or sunlight into alcohols or branched-chain hydrocarbons. In particular, the invention provides a method for producing alcohols or branched-chain hydrocarbons from a biomass polymer, including providing a genetically engineered host cell, culturing the host cell in a medium containing a biomass polymer as a carbon source such that recombinant nucleic acids in the cell are expressed, and extracting alcohols or branched-chain hydrocarbons from the culture. | 2012-07-26 |