23rd week of 2012 patent applcation highlights part 47 |
Patent application number | Title | Published |
20120142004 | Universal Tags With Non-Natural Nucleobases - The present invention relates to amplification primers with a universal tag and sequencing primers comprising at least one non-natural nucleobase capable of hybridizing to a complementary non-natural nucleobase. The present invention further relates to amplification methods of nucleic acid amplification and sequencing using an amplification primer with a universal tag and sequencing primers, as well as kits and solid supports comprising such primers and tags. | 2012-06-07 |
20120142005 | METHOD FOR SCREENING OF REGENERATIVE MEDICINE - A method for screening for a substance capable of regulating the regeneration, proliferation or differentiation of a cell or an organ, which comprises the steps of: (1) allowing a cell having a regenerative, proliferative or differentiative capability to form an embryoid body; (2) treating the embryoid body produced in step (1) with a digestive enzyme to prepare single cells from the embryoid body; (3) seeding the cells prepared in step (2) onto an adhesive plate, and adding a candidate substance to the plate to perform adhesion culturing of the cells on the plate; (4) conducting quantitative and simultaneous analysis of the levels of expression of at least two types of genes involved in the regeneration, proliferation or differentiation of cells after the adhesion culturing of step (3); and (5) evaluating the influence of the candidate substance on the regeneration, proliferation or differentiation of cells based on the results of the quantitative analysis obtained in step (4). | 2012-06-07 |
20120142006 | MASSIVE PARALLEL METHOD FOR DECODING DNA AND RNA - This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose. | 2012-06-07 |
20120142007 | STABLE COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION AND SEQUENCING - The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable DNA polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing. These compositions are useful, alone or in the form of kits, for nucleic acid amplification (e.g., by the Polymerase Chain Reaction) and sequencing (e.g., by dideoxy or “Sanger” sequencing), or for any procedure utilizing thermostable DNA polymerases in a variety of medical, forensic and agricultural applications. In particular, the compositions and methods are useful for amplifying and sequencing nucleic acid molecules that are larger than about 7 kilobases in size. | 2012-06-07 |
20120142008 | METHODS FOR EVALUATING GASTROINTESTINAL ABSORPTION OF CHEMICALS - Nucleic acids and vectors for interfering with the expression of membrane efflux transport proteins in cells that express such proteins are provided. Also provided are cells and cell lines comprising such nucleic acids and vectors. Methods for screening chemicals and biomolecules for gastrointestinal absorption in animals, and kits for practicing such methods are also provided. | 2012-06-07 |
20120142009 | COMPOSITIONS AND METHODS FOR PREVENTING OR TREATING INFLAMMATORY BOWEL DISEASE - Compositions comprising docosahexaenoic acid (DHA) and optionally one or more fatty acids selected from the group consisting of eicosapentaenoic acid (EPA), arachidonic acid (ARA), linoleic acid (LA), and α-linoleic acid (ALA) are administered to felines for preventing or treating feline inflammatory bowel disease (IBD). | 2012-06-07 |
20120142010 | MICROFABRICATED INTEGRATED DNA ANALYSIS SYSTEM - Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the sequencing template. The microcarrier element may comprise a microsphere. An autovalve at an exit port of a thermal cycling chamber, an optical scanner, or a timing arrangement may be used to ensure that only one microsphere will flow into one thermal cycling chamber wherein thermal cycling extension fragments are produced. The extension products are captured, purified, and concentrated in an integrated oligonucleotide gel capture chamber. A microfabricated component separation apparatus is used to analyze the purified extension fragments. The microfabricated structure may be used in a process for performing sequencing and other genetic analysis of DNA or RNA. | 2012-06-07 |
20120142011 | Modified siNA - The present invention pertains to the use of at least one abasic modification within the first 8 nucleotide positions of the 5′ region of the antisense strand of a small interfering nucleic acid (siNA) molecule for reducing off-target effects. Provided are suitable modified siNAs, compositions and methods for producing respective siNAs, as well as kits comprising respective siNAs. | 2012-06-07 |
20120142012 | BIOMARKER FOR COLORECTAL CANCER - The present invention relates to new methods for predicting the clinical outcome or determining the treatment course in a subject afflicted with solid tumors, like colorectal cancer, and for monitoring the progression of solid tumors, like colorectal cancer, in a subject. Moreover, the present invention relates to a method for stratification of therapy regimen of a subject afflicted with solid tumor entities, such as colorectal cancer, for determining its susceptibility to the treatment with an inhibitor of angiogenesis. Further, the present invention relates to kits allowing performance of the above methods. In particular, the present invention is based on the finding that determining the level or amount of angiopoietin-2 protein in a sample of a subject is useful for conducting the above referenced methods. | 2012-06-07 |
20120142013 | NUCLEIC ACID APTAMER CAPABLE OF BINDING SPECIFICALLY TO PANCREATIC CANCER CELLS OR TISSUES AND USE THEREOF - The present invention relates to a nucleic acid aptamer which can specifically recognize and bind to pancreatic cancer cells or tissues. The nucleic acid aptamer can bind specifically only to pancreatic cancer cells or tissues without binding to normal pancreatic cancer tissue, and thus can be effectively used as a composition for diagnosing and treating pancreatic cancer. In addition, the nucleic acid aptamer can detect not only the terminal pancreatic cancer cell line Capan-1, but also the early pancreatic cancer cell line Panc-1, and thus can be used for early diagnosis of pancreatic cancer, thereby contributing to increasing the survival rate of pancreatic cancer patients. | 2012-06-07 |
20120142014 | MOLECULAR INDICIA OF CELLULAR CONSTITUENTS AND RESOLVING THE SAME BY SUPER-RESOLUTION TECHNOLOGIES IN SINGLE CELLS - Methods and systems are provided for creating molecular barcodes or indicia for cellular constituents within single cells and for resolving such barcodes or indicia with super resolution technologies such as super resolution microscopy. By this approach, numerous molecular species that can be measured simultaneously in single cells. It has been demonstrated that multiple mRNA transcripts can be labeled with a spatially ordered sequence of fluorophores, and that barcode can be resolved. The methods and systems can be used for genome-wide transcriptional profiling in individual cells by super-resolution barcoding and suggest a general strategy to bring large-scale-omics approach into single cells. | 2012-06-07 |
20120142015 | GFP FUSION PROTEINS AND THEIR USE - The present invention provides fusion proteins including a green fluorescent protein inserted into the internal amino acid sequence of a Gαs protein and further provides method of using the fusion protein construct to follow activation of a G-protein receptor by a candidate drug. | 2012-06-07 |
20120142016 | Array-based bioactivated nanopore devices - A nanopore device capable of single molecule detection is described. The nanopores are formed in thin, rigid membranes and modified by a sputtered metal that forms an overhang during application. The overhang causes the pore to be narrower in a certain region, allowing passage of only a single molecule through the pore at a time, or binding to a biomolecule on the pore to be detected by a change in ionic current flow through the nanopore. Embodiments include a silicon nitride membrane formed on a silicon substrate and having a nanopore drilled with a focused ion beam system, followed by gold sputtering onto the membrane. Devices are formed with one or more nanopores and chambers having electrodes on either side of the nanopore. | 2012-06-07 |
20120142017 | BIOSENSOR DEVICE AND MANUFACTURING METHOD THEREOF - Disclosed is a biosensor device, comprising: a capillary tube with probe molecules immobilized on the inner wall surface thereof, and a liquid sample containing target molecules, said biosensor device being characterized in that a contact angle between the inner wall surface of the capillary tube and the liquid sample changes because of the specific interaction between the probe molecules and the target molecules, which leads, in turn, to a change in the height of the liquid sample in the capillary tube. | 2012-06-07 |
20120142018 | Rapid Single Cell Based Parallel Biological Cell Sorter - A disposable rapid cell sorter comprises of microfluidic chip with electrodes and sorts biological cells of interest through magnetic field and electric field based on biological cell functional antibody bonded magnetic beads and luminescent labeling. | 2012-06-07 |
20120142019 | Sample Metering Device and Assay Device with Integrated Sample Dilution - In one embodiment, the invention is to a sample metering device, comprising a sample holding chamber oriented between a sample entry port and a sample isolation unit and having a diluent introduction port disposed therebetween for introduction of a diluent into the sample holding chamber. The volume within the sample holding chamber between the diluent introduction port and the sample isolation unit defines a metered volume of a sample for analysis. In another embodiment, the invention is to an apparatus and method for rapid determination of analytes in liquid samples by various assays including immunoassays incorporating a sample dilution feature, preferably suitable for low range sample dilution, and preferably capable of being used in the point-of-care diagnostic field. | 2012-06-07 |
20120142020 | Sample Metering Device and Assay Device with Integrated Sample Dilution - In one embodiment, the invention is to a sample metering device, comprising a sample holding chamber oriented between a sample entry port and a sample extraction unit, wherein a portion of said extraction unit defines a metered volume of a sample. A diluent may be transported over and/or through the extraction unit to form a diluted sample for sample analysis. In another embodiment, the invention is to an apparatus and method for rapid determination of analytes in liquid samples by various assays including immunoassays incorporating a sample dilution feature, capable of being used in the point-of-care diagnostic field is provided. The devices and methods of the invention preferably are well-suited for high range sample dilution. | 2012-06-07 |
20120142021 | pH MODULATION METHODS AND SYSTEMS FOR DETECTING BINDING EVENTS - Methods and systems for detecting binding between first and second molecules using a pH-sensitive fluorophore. A change in fluorescence emission intensity of the fluorophore is indicative of binding. | 2012-06-07 |
20120142022 | GLYCOSYLATED MAMMALIAN NGAL AND USE THEREOF - The present invention relates to glycosylated mammalian NGAL, and methods of using said glycosylated mammalian NGAL. | 2012-06-07 |
20120142023 | PROTEINS AND METHOD FOR DETECTION OF LYME DISEASE - Recombinant | 2012-06-07 |
20120142024 | MEANS AND METHODS FOR THE DETERMINATION OF THE AMOUNT OF NEUROTOXIN POLYPEPTIDE AND OF ITS CATALYTIC AND PROTEOLYTIC ACTIVITIES - The present invention pertains to the field of tools for ensuring manufacture of polypeptides and quality control. Specifically, it relates to a method for determining the amount of processed (active) neurotoxin polypeptides in a solution comprising processed neurotoxin polypeptides and partially processed or unprocessed neurotoxin polypeptides. The present invention further relates to a device for determining the amount of neurotoxin polypeptides and a kit adapted to carry out the method of the present invention. | 2012-06-07 |
20120142025 | Ratiometric Immunoassay Method and Blood Testing Device - The invention is to devices and methods for rapid determination of analytes in liquid samples. The devices and methods incorporate a sample dilution feature and multiple immunosensors for performing a ratiometric immunoassay on a first analyte and a second analyte, for example, hemoglobin and hemoglobin A1c or albumin and glycosylated albumin. The devices are preferably capable of being used in the point-of-care diagnostic field. | 2012-06-07 |
20120142026 | Assay Devices with Integrated Sample Dilution and Dilution Verification and Methods of Using Same - The invention is to devices and method for rapid determination of analytes in liquid samples by various assays including immunoassays incorporating a sample dilution feature for forming a diluted sample for analysis. The devices and methods also include a dilution verification feature for verifying the degree of dilution of the diluted sample. The devices preferably are capable of being used in the point-of-care diagnostic field is provided. | 2012-06-07 |
20120142027 | Compositions and Methods for Modulating the Immune Response and Identifying Immunomodulators - The present invention provides compositions and methods for the identification of immunomodulators. The invention further provides an isolated complex comprising an UNC93B polypeptide and an UNC93B-dependent TLR polypeptide and methods of use thereof. The invention further provides methods of modulating immune system activity and methods of treating diseases characterized by aberrant immune system activity. | 2012-06-07 |
20120142028 | BIOLOGICAL MARKERS PREDICTIVE OF ANTI-CANCER RESPONSE TO EPIDERMAL GROWTH FACTOR RECEPTOR KINASE INHIBITORS - The present invention provides diagnostic methods for predicting the effectiveness of treatment of a cancer patient with an EGFR kinase inhibitor. These methods are based on the surprising discovery that the effectiveness of treatment with an EGFR kinase inhibitor is predicted by whether a patient's tumor cells express a high or a low level of the biomarkers vimentin and E-cadherin, such that patients whose tumors express a high level of at least one of the biomarkers vimentin and E-cadherin have a longer overall survival and progression free survival than patients whose tumors express a low level of both vimentin and E-cadherin. The present invention further provides a method for treating tumors or tumor metastases in a patient, comprising the steps of diagnosing a patient's likely responsiveness to an EGFR kinase inhibitor by assessing whether tumor cells express a high level of at least one of the biomarkers vimentin and E-cadherin, and administering to said patient a therapeutically effective amount of an EGFR kinase inhibitor (e.g. erlotinib), particularly when effectiveness of the inhibitor is predicted. | 2012-06-07 |
20120142029 | NUCLEIC ACIDS ENCODING A G-PROTEIN COUPLED RECEPTOR INVOLVED IN SENSORY TRANSDUCTION - The invention provides isolated nucleic acid and amino acid sequences of sensory cell specific G-protein coupled receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of sensory cell specific G-protein coupled receptors. | 2012-06-07 |
20120142030 | Biomarkers for Follicular Thyroid Carcinoma and Methods of Use - The present invention provides protein biomarkers for determining whether a thyroid nodule is malignant or benign and methods for using the same. | 2012-06-07 |
20120142031 | REAL TIME ELECTRONIC CELL SENSING SYSTEMS AND APPLICATIONS FOR CELL-BASED ASSAYS - The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays. | 2012-06-07 |
20120142032 | MULTI-FREQUENCY IMPEDANCE METHOD AND APPARATUS FOR DISCRIMINATING AND COUNTING PARTICLES EXPRESSING A SPECIFIC MARKER - An analysis method for identifying and counting particles expressing a specific antigenic marker is proposed. One example (but not exclusive) is CD4+ T-lymphocyte analysis in blood. The method comprises obtaining a sample comprising particles in suspension, labeling the particles expressing the specific marker with an impedance label, and measuring the labeled sample using a dual frequency system to discriminate at least the labeled particles expressing the specific marker. The method allows an accurate count of particles e.g. the number CD4+ T-lymphocytes in a blood flow. A corresponding device is also provided. | 2012-06-07 |
20120142033 | METHOD FOR EXTRACTION AND PURIFICATION OF RECOMBINANT PROTEINS FROM TRANSGENIC PLANTS - Extraction and purification of recombinant proteins rendered difficult to extract from transgenic plants by using an extraction solution containing reducing agents and surfactants or an extraction solution containing reducing agents and organic solvents. | 2012-06-07 |
20120142034 | PVDF MEMBRANES - The invention provides a method of treating a polyvinyl difluoride (PVDF) membrane comprising:
| 2012-06-07 |
20120142035 | Calibrator/Control for Simultaneous Assay of Proteins Capable of Complexing With One Another - Disclosed herein are compositions and methods comprising two or more proteins in which at least one of the proteins has been altered to reduce their mutual recognition and binding. Such compositions are useful as reference, calibrators or controls in methods and assays for determining the amount of one or more of the proteins that may be present in a sample of interest or in confirming the presence of one or more of the proteins in the sample. More particularly, it relates to compositions and methods comprising altered placental growth factor-1 (PlGF-1) and soluble fms-like tyrosine kinase (sFlt-1) and methods for determining the amount or confirming the presence of sFlt-1 and/or PlGF-1 in a sample of interest. | 2012-06-07 |
20120142036 | Enhanced Cleavage of Von Willebrand Factor by ADAMTS13 - Novel methods are described for measuring the rate of ADAMTS13-mediated cleavage of von Willebrand Factor (VWF) multimers. Through the use of the reagent, ristocetin, the method can advantageously be performed in the absence of an applied shear stress or added denaturing agent. Also described are methods for diagnosing ADAMTS13-associated disorders and methods for providing improved treatment of those disorders by evaluating the efficacy of the treatment using the methods as described. | 2012-06-07 |
20120142037 | GLUCOSE DEHYDROGENASE - A modified pyrroloquinoline quinone glucose dehydrogenase that exhibits a high selectivity for glucose is provided. A modified pyrroloquinoline quinone glucose dehydrogenase is disclosed in which the amino acid residue G at Position 99 of a pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) represented by SEQ ID NO: 1, or the amino acid residue G at Position 100 of the pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) represented by SEQ ID NO: 3, is substituted by the amino acid sequence TGZN (where Z is SX, S, or N and X is any amino acid residue). The modified PQQGDH of the present invention may additionally comprise one or more mutations selected from the group consisting of Q192G, Q192A, or Q192S; L193X; E277X; A318X; Y367A, Y367F, or Y367W; G451C; and N452X (where X is any amino acid residue). | 2012-06-07 |
20120142038 | KITS FOR ASSAYING ENZYME-MEDIATED OXIDATIVE DEMETHYLATION - Methods of assaying enzyme-mediated oxidative demethylation are provided according to embodiments of the present invention which includes combining, under reaction conditions, an oxidative demethylation enzyme, a substrate for the oxidative demethylation enzyme and a formaldehyde detection reagent. Detection of fluorescence is indicative of formaldehyde generated by oxidative demethylation of the substrate by the enzyme, the fluorescence resulting from reaction of formaldehyde and the formaldehyde detection reagent. | 2012-06-07 |
20120142039 | METHOD FOR PREDICTING GLYCAEMIC RESPONSE AND USE THEREOF - The present invention relates to an in vitro method for predicting the in vivo glycaemic response to carbohydrates in a food. Provided is a method wherein a food is digested in vitro in more than one compartment. Also provided is the use of such a method for determining the suitability of a food for a subject who would benefit from a normalization of blood glucose levels. | 2012-06-07 |
20120142040 | Methods and Kits for Quantitative Methyltransferase and Demethylase Measurements - The invention provides methods and kits for characterizing the activity of a methyltransferase or demethylase. The method involves enzymatically methylating or demethylating in vitro a substrate that is a peptide fragment of a full-length polypeptide, and then non-enzymatically methylating the peptide substrate with methyl groups that differ in molecular weight from the enzymatically added or removed methyl groups. Typically, deuterated or | 2012-06-07 |
20120142041 | Methods And Kits For Measuring Toxicity And Oxidative Stress In Live Cells - Compositions and methods for the measuring toxicity, antioxidant capacity, and oxidative stress are provided. | 2012-06-07 |
20120142042 | Selective Delivery Of Molecules Into Cells Or Marking Of Cells In Diseased Tissue Regions Using Environmentally Sensitive Transmembrane Peptide - A polypeptide with a predominantly hydrophobic sequence long enough to span a membrane lipid bilayer as a transmembrane helix (TM) and comprising one or more dissociable groups inserts across a membrane spontaneously in a pH-dependant fashion placing one terminus inside cell. The polypeptide conjugated with various functional moieties delivers and accumulates them at cell membrane with low extracellular pH. The functional moiety conjugated with polypeptide terminus placed inside cell are translocated through the cell membrane in cytosol. The peptide and its variants or non-peptide analogs can be used to deliver therapeutic, prophylactic, diagnostic, imaging, gene regulation, cell regulation, or immunologic agents to or inside of cells in vitro or in vivo in tissue at low extracellular pH. | 2012-06-07 |
20120142043 | METHOD FOR AUTOMATIC DETERMINATION OF SAMPLE - A method of determining a kind of a sample, in a method for analyzing a substance by the steps of supplying the sample to be analyzed to a reaction system by a supplying means comprising a transparent region composed of a transparent material, reacting a reagent for detecting the substance with the sample in the reaction system, and analyzing a signal derived from a product obtained by the reaction, characterized by irradiating the transparent region with light in the supplying step, and analyzing an optical intensity of the light. | 2012-06-07 |
20120142044 | METHODS FOR IONOPHORICALLY SCREENING PORE FORMING BACTERIAL PROTEIN TOXINS AND RECEPTORS - One embodiment of the present invention is directed to methods for ionophorically screening pore forming bacterial protein toxins and receptors for insect toxicity. The method includes: a) forming a membrane comprising a lipid and an insect receptor, b) contacting the membrane with the pore forming bacterial protein toxin and an ion solution, and c) measuring ion flow through the membrane. Also provided are a method and kit for determining the amount of live pore forming bacterial toxin protein in a sample. | 2012-06-07 |
20120142045 | USE OF AN AMORPHOUS SILICON LAYER AND ANALYSIS METHODS - A method of detecting substances or reactions of substances in a sample, comprising: (i) providing a layer (CS) based on hydrogenated or unhydrogenated amorphous silicon with attached probes, (ii) bringing the layer (CS) in contact with the sample that may contain the substances that bind specifically to or reacts specifically with the probes, under appropriate conditions for the substances to bind to or react with the probes; (iii) optionally removing non-specifically bound or non-specifically reactive substances; and (iv) detecting the presence or amount of the specifically bound or reactive substances in the sample by surface plasmon resonance (SPR) and/or fluorescence, is described. | 2012-06-07 |
20120142046 | Yeast Cells Expressing An Exogenous Cellulosome And Methods of Using The Same - The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction. | 2012-06-07 |
20120142047 | Purification and Separation Treatment Assembly (PASTA) for Biological Products - An assembly capable of capturing and purifying expressed biological products during or at the end of a bioreaction cycle is disclosed wherein a binding resin is kept separated from the contents of the bioreactor allowing capturing, harvesting and purification of biological products in a bioreactor; the invention additionally provides means of removing undesirable metabolic products as well as provides for efficient loading of chromatography columns. | 2012-06-07 |
20120142048 | NUCLEIC ACID WHICH IS STABILIZED AGAINST DECOMPOSITION - The invention relates to a nucleic acid which is stabilised against decomposition by exonucleases. Said nucleic acid contains the following constituents: a) a code sequence coding for a defined protein, b) optimally, a promoter sequence controlling the expression of the code sequence, and c) at least one molecule A added to an end of the linear sequence containing the constituents a and b, said molecule being linked to a non-immobilised, volumic molecule B. | 2012-06-07 |
20120142049 | Recombinant Gene Expression - Genes are expressed by culturing cells comprising a host chromosome comprising an integrated artificial chromosome comprising recombinant genes, under conditions whereby each recombinant gene is expressed copy number dependently and position independently. Deletions increase expression from recombinant gene(s) inserted into the artificial chromosome. | 2012-06-07 |
20120142050 | NOVEL EXPRESSION VECTOR - The present inventors constructed a transformation system which uses as a host the | 2012-06-07 |
20120142051 | TRANSCRIPTION REGULATORY FACTORS FOR MANNANASES OR CELLULASES, AND GENES FOR THE TRANSCRIPTION REGULATORY FACTOR - Disclosed are: transcription regulatory factors capable of regulating the transcription or expression of genes for mannanases or cellulases, as mentioned below; and others. Specifically disclosed is a protein selected from the following proteins (a), (b) and (c): (a) a protein comprising the amino acid sequence depicted in SEQ ID NO:2; (b) a protein which comprises an amino acid sequence produced by deleting, substituting or adding one or several amino acid residues (e.g., 1 to 5 amino acid residues) in the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases; and (c) a protein which comprises an amino acid sequence having a 70% or higher sequence identity to the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases, or a partial fragment of the protein. Also specifically disclosed are a gene encoding the protein, and others. | 2012-06-07 |
20120142052 | METHOD AND DEVICE FOR MULTIPLE FEED CULTIVATION - The current invention is directed to support units for mounting a cell cultivation liquid storage unit which can be connected to a balance, a cell cultivation apparatus using such a support unit, and the use of such a support unit in a method for the cultivation of cells. | 2012-06-07 |
20120142053 | METHOD FOR METHANOL INDEPENDENT INDUCTION FROM METHANOL INDUCIBLE PROMOTERS IN PICHIA - A method for producing a polypeptide in a methylotrophic yeast host cell is described, where expression of the polypeptide is controlled by a methanol inducible promoter, including: i) expression of a positive regulator from a non-native promoter, the positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol. | 2012-06-07 |
20120142054 | SRSV DETECTION KIT - A polynucleotide base sequence represented by SEQ ID NO: 22, a vector containing the polynucleotide and a method of preparing a small round structure virus (SRSV) virus-like particle in insect cells with vector. | 2012-06-07 |
20120142055 | TARGETED INTEGRATION INTO THE PPP1R12C LOCUS - Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into the human PPP1R12C locus, for example, for expression of a polypeptide of interest. | 2012-06-07 |
20120142056 | MAMMALIAN EXPRESSION VECTOR pUHAB - The present invention relates to the construction and utilization of a new mammalian expression vector that contains a unique multiple cloning site (MCS), designated pUHAB. The pUHAB vector comprises a high copy replication origin (ColE1), a drug resistance gene (TK-Hygromycin), and a human cytomegalovirus promoter operably associated with a unique intron (hCMV/intron). Further, pUHAB comprises a selectable marker conferring resistance to kanamycin in bacterial cells, and a phage f1(+) region. pUHAB can be used to transiently or stably express cloned genes when transfected into mammalian cells. The invention also encompasses kits and host cells and cell lines comprising pUHAB, and methods of producing a recombinant protein using pUHAB. | 2012-06-07 |
20120142057 | METHOD FOR PRODUCTION OF POLYPEPTIDE - The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses cysteine sulfinic acid decarboxylase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide. Hamster cysteine sulfinic acid decarboxylase, a DNA encoding the same, a recombinant vector and a transformed cell are also provided. | 2012-06-07 |
20120142058 | Conversion of Chitin into N-Acetylglucosamine, Glucosamine and Bioethanol - Compositions and methods are provided for converting chitin into N-acetylglucosamine, glucosamine and ethanol. The chitin may be used directly from the environment, for example, as occurs in invertebrate cuticles, fungal cells and/or algae. Mutant bacteria were created by knocking out or inactivating one or more genes preferably resulting in the chitin catabolic sensor maintaining an activated state. Methods are further provided for converting the N-acetylglucosamine into ethanol by means of a genetically engineered yeast strain which can be optionally co-cultivated with the Vibrionaceae to produce significant yields of ethanol. | 2012-06-07 |
20120142059 | SEQUENCE AMPLIFICATION WITH TARGET PRIMERS - The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. This can be accomplished via the use of target primers and isothermal multiple strand displacement (MDA) processes. The use of these target primers and MDA, as described herein, allows for the reduction in the amplification of undesired hybridization events (such as primer dimerization and the “jackpot mutation” effect of PCR) while allowing for the amplification of the target nucleic acid sequences. | 2012-06-07 |
20120142060 | Compositions and Methods for Processing and Amplification of DNA, Including Using Multiple Enzymes in a Single Reaction - The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome. | 2012-06-07 |
20120142061 | Recombinant Colwellia Psychrerythraea Alkaline Phosphatase and Uses Thereof - A heat labile alkaline phosphatase enzyme and methods of using the same and kits including the same are disclosed. Specifically, a nucleotide sequence of, peptide sequence of, methods of using, and kits comprising, a heat labile alkaline phosphatase isolated from | 2012-06-07 |
20120142062 | ENGINEERED CLEAVAGE HALF-DOMAINS - Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence. | 2012-06-07 |
20120142063 | NITRILASE BIOCATALYSTS - The present disclosure relates to polypeptides having nitrilase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides. | 2012-06-07 |
20120142064 | RECOMBINANT BACTERIA HAVING THE ABILITY TO METABOLIZE SUCROSE - Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct: a nucleotide sequence from | 2012-06-07 |
20120142065 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation. | 2012-06-07 |
20120142066 | Metabolically Enhanced Photoautotrophic Ethanol Producing Host Cells, Method For Producing The Host Cells, Constructs For The Transformation Of The Host Cells, And Method Of Producing Ethanol Using The Host Cells - One embodiment of the invention provides a metabolically enhanced photoautotrophic, ethanol producing host cell comprising:
| 2012-06-07 |
20120142067 | METHOD FOR PREPARING AN INDUSTRIAL YEAST, INDUSTRIAL YEAST, AND APPLICATION TO THE PRODUCTION OF ETHANOL FROM AT LEAST ONE PENTOSE - The present invention relates to the field of the methods for obtaining yeast strains producing ethanol, of the thereby produced strains, and of the industrial production of ethanol from said strains. | 2012-06-07 |
20120142068 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation. | 2012-06-07 |
20120142069 | INTERPENETRATING BIOMATERIAL MATRICES AND USES THEREOF - The present invention relates to matrices (e.g., fibrin-alginate matrices; fibrin-alginate-matrigel matrices) for culture of cells, organs (e.g., ovary or fragment thereof), cells and cell aggregates (e.g., ovarian follicles, embryoid bodies), and tissues. In some embodiments, protease inhibitors e.g., aprotinin) are used to prevent the degradation of fibrin. | 2012-06-07 |
20120142070 | REHYDRATABLE MATRICES FOR DRY STORAGE OF TAQ POLYMERASE IN A MICROFLUIDIC DEVICE - Formulations for dry storage of PCR reagents are described. These formulations find use in manufacture of self-contained microfluidic card devices for PCR clinical testing in which the reagents are reconstituted at the point of testing. In these cards, TAQ polymerase is stored “on-board” in vitrified dry form without lyophilization or freezing, and is reconstituted by either the sample or a sample eluate during the assay. | 2012-06-07 |
20120142071 | Thymidine Kinase Mutants - The present invention provides isolated nucleic acid molecules encoding a Herpesviridae thymidine kinase enzyme comprising one or more mutations, at least one of the mutations encoding an amino acid substitution located toward the N-terminus from a DRH nucleoside binding site which increases a biological activity of the thymidine kinase, as compared to unmutated thymidine kinase. Such mutations include amino acid substitutions within a Q substrate binding domain which increases a biological activity of the thymidine kinase, as compared to unmutated thymidine kinase. Within a further aspect, fusion proteins are provided which have both guanylate kinase and thymidine kinase biological properties. Also provided are vectors suitable for expressing such DNA molecules, as well as methods for utilizing such vectors. | 2012-06-07 |
20120142072 | SOLUBLE PROTEIN AND UTILIZATION OF THE SAME - Disclosed is a method for screening a medicine using a protein, and a compound obtained by the screening method. A compound or a salt thereof inhibiting activity or expression of the protein of the present invention, a neutralizing antibody against the protein, a polynucleotide that are complementary to a polynucleotide coding the protein, and the like can be used as an agent for preventing and/or treating neurodegenerative diseases and the like. Moreover, a compound or a salt thereof enhancing activity or expression of the protein of the present invention, the protein or a partial peptide thereof, a polynucleotide coding the protein, and the like can be used as an agent for preventing and/or treating cancers and the like. Furthermore, the protein of the present invention is useful as a reagent for screening a compound inhibiting or enhancing activity of the protein. | 2012-06-07 |
20120142073 | HIDE PROCESSING METHODS AND COMPOSITIONS - Provided in part herein are compositions that include isolated microbial enzymes, such as proteases, lipases, useful in dehairing leather. Also provided are methods for isolating proteases, lipases, from microbes, as well as methods of reusing these enzymes effectively for several cycles in an environment friendly process. | 2012-06-07 |
20120142074 | Compositions and Uses for an Alpha-Amylase Polypeptide of Bacillus Species 195 - Disclosed herein are compositions comprising an alpha-amylase enzyme obtained from | 2012-06-07 |
20120142075 | Microbial Trypsin Mutants having Chymotrypsin Activity And Nucleic Acids Encoding Same - The present invention relates to microbial trypsin variants having chymotrypsin-like activity, comprising: (a) a one or more substitutions corresponding to positions 144, S193, 198, 201, 218, 223, 227, 228, 229, 230, and 231 of amino acids 25 to 248 of SEQ ID NO: 2, (b) one or more deletions corresponding to positions 192, 197, and 226 of amino acids 25 to 248 of SEQ ID NO: 2; and (c) an insertion between positions corresponding to positions 224 and 225 of amino acids 25 to 248 of SEQ ID NO: 2. The present invention further relates to nucleotide sequences encoding microbial trypsin variants having chymotrypsin-like activity; nucleic acid constructs, expression vectors, and recombinant host cells comprising such nucleotide sequences; and methods of producing microbial trypsin variants having chymotrypsin-like activity or a precursor thereof. | 2012-06-07 |
20120142076 | BACILLUS STRAIN FOR INCREASED PROTEIN PRODUCTION - The present invention provides host cells that have been genetically manipulated to have an enhanced capacity to produce proteins of interest. In particular, the invention relates to modified | 2012-06-07 |
20120142077 | SYNTHETIC ENZYMES DERIVED FROM COMPUTATIONAL DESIGN - Disclosed herein are techniques for computationally designing enzymes. These techniques can be used to design variations of naturally occurring enzymes, as well as new enzymes having no natural counterparts. The techniques are based on first identifying functional reactive sites required to promote the desired reaction. Then, hashing algorithms are used to identify potential protein backbone structures (i.e., scaffolds) capable of supporting the required functional sites. These techniques were used to design 32 different protein sequences that exhibited aldol reaction catalytic function, 31 of which are defined in the Sequence Listing. Details of these 31 different synthetic aldolases are provided, including descriptions of how such synthetic aldolases can be differentiated from naturally occurring aldolases. | 2012-06-07 |
20120142078 | SYSTEM FOR GENERATION OF VIABLE REOVIRUS FROM CLONED CDNA - The present invention provides methods for the generation of viable reoviruses using only cloned nucleic acid segments representing the RNA segments of the reovirus genome. | 2012-06-07 |
20120142079 | MINICELL BASED DELIVERY OF BIOLOGICALLY ACTIVE COMPOUNDS - Minicells are used to deliver biologically active compounds including radioisotopes, polypeptides, nucleic acids, small molecules, drug molecules, and chemotherapeutic agents. In some cases, the minicell displays ligands or binding moieties that target the minicell to a desired host cell. | 2012-06-07 |
20120142080 | LIVE BACTERIAL VACCINES FOR VIRAL INFECTION PROPHYLAXIS OR TREATMENT - A live bacterium, having a DNA construct stabilized against transduction of other bacteria, having a promoter sequence and encoding a fusion peptide, comprising a bacterial secretion peptide portion and a non-bacterial immunogenic polypeptide portion, having a nucleotide sequence coding for the non-bacterial immunogenic polypeptide portion which has at least one codon optimized for bacterial expression. The bacterium has a secretion mechanism which interacts with at least the bacterial secretion peptide portion to cause a secretion of the fusion peptide from the bacterium, and a genetic virulence attenuating mutation. The bacterium is adapted to act as an animal vaccine, to transiently infect a tissue of the animal, and cause an immunity response to the non-bacterial immunogenic polypeptide portion in the animal to a non-bacterial organism associated with the non-bacterial immunogenic polypeptide portion. | 2012-06-07 |
20120142081 | BIOSYNTHESIS OF COMMODITY CHEMICALS - Methods, enzymes, recombinant microorganism, and microbial systems are provided for converting suitable monosaccharides or oligosaccharides, such as those derived from biomass, as well as various aldehydes and/or ketones, into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided. Commodity chemical enriched, refinery-produced petroleum products are also provided, as well as methods for producing the same. | 2012-06-07 |
20120142082 | CAROTENOID PRODUCTION IN A RECOMBINANT OLEAGINOUS YEAST - Engineered strains of the oleaginous yeast | 2012-06-07 |
20120142083 | BIOFILTER - A biofilter for use in treating contaminated gases is provided with a container having a layer of rubber particulate, which serves as a filter media. One preferred source of the rubber particulate is recycled tires. The rubber particulate provides a platform for the growth and maintenance of a microbial ecosystem that substantially treats the contaminated gases emanating from the waste. A plurality of different embodiments provide relatively simple inexpensive biofiltration solutions for a wide range of applications. A method of reducing odorous pollutants from products is also described wherein the product containing the pollutant or pollutants is passed through a layer of the rubber particulate material. | 2012-06-07 |
20120142084 | GRAPHICAL INTERFACE FOR ANALYTE METER - Diagnostic systems include a meter that is configured to receive a test sensor during a testing procedure. The diagnostic systems also include a computing device coupled to the meter. The test sensor receives a fluid sample during the testing procedure. The meter includes a measurement system that determines a measurement of a concentration of an analyte in the fluid sample. The computing device receives and processes the measurement from the meter. The computing device has enhanced processing and presentation capabilities that provide visual and/or audio instructions on how to operate the diagnostic system, especially when an error or exceptional condition arises. | 2012-06-07 |
20120142085 | SYSTEMS FOR RAPID IDENTIFICATION OF PATHOGENS IN HUMANS AND ANIMALS - The present invention provides methods of: identifying pathogens in biological samples from humans and animals, resolving a plurality of etiologic agents present in samples obtained from humans and animals, determining detailed genetic information about such pathogens or etiologic agents, and rapid detection and identification of bioagents from environmental, clinical or other samples. | 2012-06-07 |
20120142086 | IMAGING SYSTEM COMPRISING MICROLENSES AND ASSOCIATED DEVICE FOR DETECTING A SAMPLE - The invention relates to an imaging system and an associated device for detecting a sample ( | 2012-06-07 |
20120142087 | REACTOR FOR THE ANAEROBIC DIGESTION OF BIOMASS - A reactor for the anaerobic digestion of biomass, includes: a gas-tight tank that is accessible by a sealed door designed for the intake of the biomass, the tank being designed in the form of a garage; equipment for heating the biomass provided e.g. at the walls of the tank; a biogas-tapping connection; and equipment for draining the seepage liquid. According to the invention, the lower walls ( | 2012-06-07 |
20120142088 | DNA-CELL CONJUGATES - The present invention provides conjugates of DNA and cells by linking the DNA to a native functional group on the cell surface. The cells can be without cell walls or can have cell walls. The modified cells can be linked to a substrate surface and used in assay or bioreactors. | 2012-06-07 |
20120142089 | METHOD OF SEPARATING TARGET CELL IN BIOLOGICAL SAMPLE - Provided is a method of separating a target cell in a biological sample. A target cell may be efficiently separated from a biological sample including at least a second cell of similar density to the target cell using the disclosed method of separating a target cell in a biological sample according embodiment. | 2012-06-07 |
20120142090 | Protein Expression System - The present invention relates to a method of producing a target protein, which method comprises expressing said protein in a host cell which contains a nucleic acid molecule which encodes a chimeric protein, said chimeric protein comprising a signal peptide from a non-mammalian bulk-secreted protein and said target protein; nucleic acids, vectors, host cells and kits for carrying out the method are also described. | 2012-06-07 |
20120142091 | Antibodies Against Human Angiopoietin 2 - The present invention relates to antibodies against human Angiopoietin 2 (anti-ANG-2 antibodies), methods for their production, pharmaceutical compositions containing said antibodies, and uses thereof. | 2012-06-07 |
20120142092 | RAT EMBRYONIC STEM CELL - The present invention provides a rat embryonic stem cell characterized by having the following properties of (a) expressing Oct3/4 gene and Nanog gene, (b) positive for alkaline phosphatase activity, (c) having an embryoid body forming ability, (d) expressing SSEA (Stage-Specific Embryonic Antigen)-1 and SSEA-4, (e) having the same number of chromosomes as does a normal rat cell, (f) capable of being subcultured and holding the undifferentiated state, (g) having in vitro pluripotency, (h) having a potential to differentiate for cells of three embryonic germ lineages, (i) having teratoma formation ability, and (j) having an ability to produce a chimeric rat, a method of establishing the aforementioned rat embryonic stem cell and the like. | 2012-06-07 |
20120142093 | METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO NEURAL PRECURSOR CELLS - The present invention provides a method for inducing differentiation of pluripotent stem cells into neural precursor cells, comprising culturing the pluripotent stem cells in the presence of a small molecule BMP inhibitor, and induced neural precursor cells prepared by this method. | 2012-06-07 |
20120142094 | GENERATING IPS CELLS BY PROTEIN TRANSDUCTION OF RECOMBINANT POTENCY-DETERMINING FACTORS - The present invention relates generally to compositions and methods for reprogramming a primate somatic cell to a higher potency level. Specifically, the invention includes compositions which comprise a recombinant polypeptide that is a potency-determining factor and methods of reprogramming a primate somatic cell to a higher potency level under conditions that allow sufficient amount of the polypeptide delivered into the primate somatic cell. | 2012-06-07 |
20120142095 | TECHNIQUE FOR DETERMINING THE STATE OF A CELL AGGREGATION IMAGE PROCESSING PROGRAM AND IMAGE PROCESSING DEVICE USING THE TECHNIQUE, AND METHOD FOR PRODUCING A CELL AGGREGATION - An image processing program (GP) attains (S | 2012-06-07 |
20120142096 | FUSION PROTEINS OF HIV REGULATORY/ACCESSORY PROTEINS - The invention relates to fusion proteins comprising the amino acid sequence of at least three HIV proteins selected from Vif, Vpr, Vpu, Rev, and Tat or derivatives of the amino acid sequence of one or more of said proteins, wherein the fusion protein is not processed to individual HIV proteins having the natural N and C termini. The invention further concerns nucleic acids encoding said proteins, vectors comprising said nucleic acids, and methods for producing said proteins. The fusion protein, nucleic acids and vectors are usable as vaccines for the at least partial prophylaxis against HIV infections. | 2012-06-07 |
20120142097 | FUSION PROTEINS OF HIV REGULATORY/ACCESSORY PROTEINS - The invention relates to fusion proteins comprising the amino acid sequence of at least three HIV proteins selected from Vif, Vpr, Vpu, Rev, and Tat or derivatives of the amino acid sequence of one or more of said proteins, wherein the fusion protein is not processed to individual HIV proteins having the natural N and C termini. The invention further concerns nucleic acids encoding said proteins, vectors comprising said nucleic acids, and methods for producing said proteins. The fusion protein, nucleic acids and vectors are usable as vaccines for the at least partial prophylaxis against HIV infections. | 2012-06-07 |
20120142098 | Fc receptor-binding polypeptides with modified effector functions - Disclosed are processes for producing a variant polypeptide (e.g. antibodies) having modified binding characteristics for human Fc gamma receptor IIA (CD32A) leading to increased inhibition of proinflammatory mediators while retaining binding to a target antigen via its Fv portion, which processes comprise altering the polypeptides by substitution of at least two amino acid residues at EU position 325, 326 or 328 of a human IgG CH2 region for a sequence selected from SAAF, SKAF, NAAF and NKAF. Also disclosed are molecules, particularly polypeptides, more particularly immunoglobulins (e.g. antibodies) that include a variant CDR3 region, wherein the variant CDR3 region includes at least one amino acid modified relative to a wild-type CDR3 region. The polypeptides that can be generated according to the methods of the invention are highly variable, and they can include antibodies and fusion proteins that contain an Fc region or a biologically active portion thereof. | 2012-06-07 |
20120142099 | NOVEL BAK BINDING PROTEIN, DNA ENCODING THE PROTEIN, AND METHODS OF USE THEREOF - The present invention provides polynucleotide sequences (bbp) encoding a Bak Binding Protein (BBP) and fragments thereof that bind to Bak. The invention also provides a BBP which binds to Bak. The invention also provides recombinant host cells containing polynucleotides encoding BBP. The invention further provides antibodies that specifically bind to BBP. The invention further provides methods for detecting agents such as drugs that alter the binding of a BBP with a Bak protein. The invention further provides methods for detecting the presence of bbp or BBP in a biological sample, and further provides methods for modulating the levels of BBP in a cell. This invention additionally encompasses novel peptides, designated the “BBP Binding Domains” and the respective nucleotides, designated “bbpbd-1” and “bbpbd-2” which are involved in the interaction between Bak and BBP. | 2012-06-07 |
20120142100 | HEAT SHOCK RNA FROM VARIOUS SPECIES AND ITS USE IN ACTIVATION OF HEAT SHOCK TRANSCRIPTION FACTOR, IN TREATMENT OF VARIOUS DISEASES IN ANIMALS AND IN GENERATION OF STRESS-RESISTANT PLANTS - The present invention provides a novel RNA, designated herein as the “HSR1” (Heat Shock RNA), and its use together with translation elongation factor eEF1A in activation of heat shock transcription factor HSF. The invention further provides the use of HSR1 for generation of novel therapeutics for the treatment of various diseases in animals and for generation of stress-resistant plants. | 2012-06-07 |
20120142101 | OLIGONUCLEOTIDE END CAPS - Modified nucleic acids are described herein, including pharmaceutical compositions comprising the modified nucleic acids, and methods of using the modified nucleic acids. | 2012-06-07 |
20120142102 | ISOLATION OF HUMAN UMBILICAL CORD BLOOD-DERIVED MESENCHYMAL STEM CELLS - Human umbilical cord blood (UCB) contains mesenchymal stem cells (MSCs) that have higher multipotentiality than adult marrow-derived MSCs. However, it has been difficult to obtain these cells because the frequency of MSCs in UCB is extremely rare (0.4-30 out of 1×10 | 2012-06-07 |
20120142103 | METHOD FOR INDUCING DIFFERENTIATION INTO EPITHELIAL PROGENITOR CELL/STEM CELL POPULATION AND CORNEAL EPITHELIAL CELL POPULATION FROM INDUCED PLURIPOTENT STEM CELLS - The present invention relates to: a method for inducing differentiation into an epithelial progenitor cell/stem cell population or a corneal epithelial cell population by culturing, under particular conditions, induced pluripotent stem cells induced from mammalian somatic cells or undifferentiated stem cells; an epithelial progenitor cell/stem cell population or a corneal epithelial cell population obtained by the method; and a cell preparation for the treatment of epithelial disease and a cell sheet, which are prepared using these cell populations. | 2012-06-07 |