22nd week of 2011 patent applcation highlights part 36 |
Patent application number | Title | Published |
20110129838 | USING GENETIC POLYMORPHISMS OF THE BICD1 GENE AS A METHOD FOR DETERMINING A RISK OF DEVELOPING MYOPIA - A method and kit for determining an increased risk of developing myopia in a subject is provided by detecting an SNP in the BICD1 gene. The SNP is selected from a group consisting of rs10844126 (A/C), rs1151029 (A/T), rs2650122 (C/T), rs10771923 (A/G), rs1151009 (T/C), rs2125173 (A/G) and rs161959 (C/G). When the presence of the risk allele associated with myopia is detected at the SNP, the subject is determined in an increased risk of developing myopia. | 2011-06-02 |
20110129839 | DEVICE, KIT AND METHOD FOR PULSING BIOLOGICAL SAMPLES WITH AN AGENT AND STABILISING THE SAMPLE SO PULSED - The present invention is related to a device, kit and method for pulsing a biological sample with a pulsing agent and subsequently stabilizing the biological sample so pulsed. The invention has application in the fields of medical diagnostics, particularly relating to immunity. | 2011-06-02 |
20110129840 | METHOD, PROCESS, AND KIT FOR DIAGNOSIS OR PROGNOSIS OF COLORECTAL CANCER - A process or method and a kit for the diagnosis/prognosis and follow-up of colorectal cancer, which includes a) the extraction of nucleic material from a biological sample, b) use of at least one pair of amplification primers in order to obtain amplicons of at least one target sequence, and c) use of at least one detection probe to detect the presence of the amplicons, the process or method including a modification step between steps 1) and b). Steps b) and c) are carried out at the same time. The target sequence is included in the WIF1 gene and the pair of primers includes at least one amplification primer including at least 15 nucleotide patterns of a nucleotide sequence selected from SEQ ID nos. 1 to 10 and/or the detection probe includes at least 15 nucleotide patterns of a nucleotide sequence selected from SEQ ID nos. 1 to 10. | 2011-06-02 |
20110129841 | ANALYSIS USING MICROFLUIDIC PARTITIONING DEVICES - The invention relates to methods, reagents and devices for detection and characterization of nucleic acids, cells, and other biological samples. Assay method are provided in which a sample is partitioned into sub-samples, and analysis of the contents of the sub-samples carried out. The invention also provides microfluidic devices for conducting the assay. The invention also provides an analysis method using a universal primers and probes for amplification and detection. | 2011-06-02 |
20110129842 | CYANOBACTERIA SAXITOXIN GENE CLUSTER AND DETECTION OF CYANOTOXIC ORGANISMS - The present invention relates to methods for the detection of cyanobacteria, dinoflagellates, and in particular, methods for the detection of cyanotoxic organisms. Kits for the detection of cyanobacteria, dinoflagellates, and cyanotoxic organisms are provided. The invention further relates to methods of screening for compounds that modulate the activity of polynucleotides and/or polypeptides of the saxitoxin and cylindrospermopsin biosynthetic pathways. | 2011-06-02 |
20110129843 | METHOD FOR EVALUATING THE VIRULENCE OF PATHOGENIC BIPHASIC BACTERIA - A method for evaluating relative bacterial virulence of a biphasic bacteria in environmental systems includes measuring the concentration of DNA in the bacteria, measuring the concentration of RNA in the bacteria, determining a ratio of the concentration of RNA to the concentration of DNA and correlating the concentration ratio with a level of relative pathogenicity, wherein the bacteria is preferentially | 2011-06-02 |
20110129844 | Use of Activating Transcription Factor-2 (ATF2) for Detecting Skin Cancer - The present invention is based on the discovery of the tumor suppressor role of activating transcription factor 2 (ATF2) in non-melanoma skin cancer development. Accordingly, the invention provides methods of diagnosing a subject as having or at risk of having skin cancer. Also provided are methods of treating skin cancer in a subject, characterizing skin cancer in a subject, and identifying agents useful for treating skin cancer in a subject. | 2011-06-02 |
20110129845 | METHOD OF DIAGNOSIS - The present invention relates generally to a method of diagnosing, predicting and/or monitoring the development or progress of an airway tissue inflammatory response in a mammal. More particularly, the present invention relates to a method of diagnosing, predicting and/or monitoring the development or progress of an airway tissue inflammatory response by analysis of activin and/or follistatin levels in the breath condensate of said mammal. The present invention further provides a method for predicting, diagnosing and/or monitoring conditions associated with or characterised by the onset of an airway tissue inflammatory response. Also provided are diagnostic agents useful for detecting activin expression levels. | 2011-06-02 |
20110129846 | PHOTONIC BIOSENSOR, PHOTONIC BIOSENSOR ARRAY, AND METHOD OF DETECTING BIOMATERIALS USING THE SAME - A photonic biosensor, a photonic biosensor array, and a method of detecting a bio-material using the same are provided. The photonic biosensor includes a light emitting diode configured to emit light, a photodiode (PD), an optical fiber configured to connect the light emitting diode with the PD, and a micro-fluidic channel disposed on the optical fiber. Bio-antibodies or aptamers are fixed to the surface of the optical fiber, and the micro-fluidic channel includes gold (Au) nanoparticles to which bio-antibodies or aptamers are fixed. The photonic biosensor may be configured using absorption of surface plasmons in Au nanoparticles with respect to light traveling through the surface of the optical fiber configured to connect the light emitting diode with the PD, thus simplifying the manufacture of the biosensor and reducing the manufacturing cost. | 2011-06-02 |
20110129847 | MICROFLUIDIC CONTROL CHIP AND METHOD OF DETECTING PROTEIN USING THE SAME - Provided is a microfluidic control chip, which includes a filter section having a filter to which anti-immunoglobulin antibodies, which are bound to endogenous antibodies in blood to thereby remove the endogenous antibodies, are immobilized, a first reaction section to which detection antibodies immobilized to fluorescent nano-particles are adsorbed, the detection antibodies being bound to proteins to be detected in blood which is introduced from the filter section with the endogenous antibodies removed therefrom, and a second reaction and detection section including capture antibodies immobilized thereto, binding the capture antibodies to the proteins, which are bound to the detection antibodies introduced from the first reaction section, and detecting a concentration of the proteins based on an intensity of fluorescent light. Thus, the microfluidic control chip can minimize interference of an immune response to maximize the immune response. | 2011-06-02 |
20110129848 | Antibody Recognizing G Protein, and Agent and Kit Using the Same - A novel protein (Gm1) includes an amino acid sequence part having a high homology with a domain having a high homology with a GTP binding site and a GTPase site conserved among G protein α subunits and a trimer forming domain conserved among G protein α subunits. The Gm1 protein is involved in signal transduction via a G protein-coupled receptor (GPCR) stimulation. The Gm1 protein is expressed intensively in human brain, thymus, testes, spleen, small intestine, uterus and heart. A method for screening for a substance capable of regulating a cellular signal transduction employs a polynucleotide encoding the Gm1 protein | 2011-06-02 |
20110129849 | DETECTION OF PROSTATE CANCER USING PSA GLYCOSYLATION PATTERNS - The present invention features novel methods for determining if a subject has prostate cancer. The present invention is based on the development of lectin immunosorbant assays which analyze α2,6-linked sialylation of total serum PSA by | 2011-06-02 |
20110129850 | MICROFLUIDIC PLATFORM FOR CELL CULTURE AND ASSAY - A microfluidic chip for at least one of cell culturing and cell assay has a cell culture chamber defined by the microfluidic chip, a first microchannel defined by the microfluidic chip and constructed to provide a fluid path to said cell culture chamber, the microchannel having a pneumatic valve formed therein to permit selective opening and closing of a fluid path to said cell culture chamber, and a second microchannel defined by the microfluidic chip and constructed to provide a fluid path from the cell culture chamber. | 2011-06-02 |
20110129851 | Methods for Obtaining Bioactive Compounds from Phytoplankton - Phytoplankton represent a potential source of bioactive compounds. The present disclosure provides, inter alia, methods for identifying glycerolipids and apoptosis-inducing sphingosine-like lipids from virally-infected phytoplankton. | 2011-06-02 |
20110129852 | ORGANIC COMPOUNDS - The present invention relates to isolated polypeptides which comprise an amino acid sequence consisting of a mutated functional Abl kinase domain, said mutated functional kinase domain being resistant to inhibition of its tyrosine kinase activity by N-[4-methyl-3-(4-pyridin-3-yl-pyrimidin-2-ylamino)-phenyl]-4-(4-methyl-piperazin-1-ylmethyl)-benzamide or a salt thereof, to the use of such polypeptides to screen for compounds which inhibit the tyrosine kinase activity of such polypeptides, to nucleic acid molecules encoding such polypeptides, to recombinant vectors and host cells comprising such nucleic acid molecules and to the use of such nucleic acid molecules in the production of such polypeptides for use in screening for compounds which inhibit the tyrosine kinase activity of such polypeptides. | 2011-06-02 |
20110129853 | POLYSACCHARIDE-PROTEIN BINDING MODEL AND NANO-FIBRIL FORMATION OF A STARCH BINDING DOMAIN - A polysaccharide-protein binding model of SBD, and a fibril-forming 14-residue peptide consisting of X | 2011-06-02 |
20110129854 | ASSAY FOR DIAGNOSIS OF CARDIAC MYOCYTE DAMAGE - Assays are disclosed for diagnosing a clinical condition, assessing risk or predicting an outcome as a result of cardiac myocyte damage. Immunoassay methods and kits provide for the assessment of cardiac myocyte damage by determining the presence of multiple cardiac myocyte antigens in a test sample, and combining the multiple determinations in a single assay result. | 2011-06-02 |
20110129855 | Recombinant Anti-Epidermal Growth Factor Receptor Antibody Compositions - The invention relates to the field of recombinant antibodies for use in human cancer therapy. More specifically the invention provides compositions or mixtures of antibodies capable of binding human EGFR. Antibody compositions with 3 or more antibodies shown synergy in reduction of proliferation of representative cancer cell lines. Advantageous results have also been obtained with a composition comprising two different chimeric anti-hEGFR antibodies which show a new mechanism of action based on rapid and efficient receptor internalisation, induction of terminal differentiation and subsequent tumour eradication in an animal model. The antibodies of the invention can be manufactured in one bioreactor as a polyclonal antibody. | 2011-06-02 |
20110129856 | APPARATUS AND METHOD FOR SEPARATING COMPONENTS - An apparatus for separating components and a method of separating components using the apparatus are provided. The apparatus includes: a main chamber which contains a sample that is separated into a plurality of layers by a centrifugal force; a component separating chamber which is connected to the main chamber, and receives a specific layer including specific components among the plurality of layers; a first channel which connects the component separating chamber to the main chamber; and a first channel valve which is disposed in the first channel to control a liquid flowing through the first channel. | 2011-06-02 |
20110129857 | Methods Of Detecting Or Monitoring Activity Of An Inflammatory Condition Or Neurodegenerative Condition - An isolated aggrefatin protein elevated in multiple sclerosis patients as compared to healthy controls. Aggrefatin alone or in combination with other markers may be used as an indicator of an inflammatory condition and/or a neurodegenerative disease or condition such as multiple sclerosis, cancer, stroke, or other diseases. Aggrefatin alone or in combination with one or more other biomarkers may help monitor disease activity, detect a response to a therapy, or detect patient compliance with a therapy. | 2011-06-02 |
20110129858 | Prognosis Biomarker for Evaluating the Cure Level of Stroke Patient and a Method thereof - A method for evaluating the cure level of a stroke patient comprises following steps: (1) obtaining isolated blood sample from said stroke patient; (2) determining the concentration of serum granulocyte colony-stimulating factor (G-CSF) of said blood sample; (3) comparing the relationship between said concentration of granulocyte colony-stimulating factor (G-CSF) and the stroke severity ranking of said stroke patient; wherein United State National Institute of Health Stroke Scale (NIHSS) or modified Ranking Scale (mRS) is used in said stroke severity ranking; and (4) Using said concentration of granulocyte colony-stimulating factor (G-CSF) to predict the possible cure level of said stroke patient. The invention further provides a prognosis biomarker for evaluating the cure level of a stroke patient, and a kit containing said prognosis biomarker. | 2011-06-02 |
20110129859 | BIOMARKER FOR DIAGNOSIS OF LIVER DISEASE - Disclosed are: a marker for the diagnosis of a liver disease, which can determine the disease in a simple manner; an antibody directed against the marker; a diagnostic agent; a diagnosis method; and a method for marker detection in blood or serum. Proteome analysis revealed that quantities of the full-length kininogen and three partial peptides thereof (sequence A: position-440 to position-456, sequence B: position-439 to position-456, and sequence C: position-438 to position-456) in sera of patients with non-alcoholic fatty liver disease are significantly different from those in sera of healthy individuals; and a diagnostic agent and a detecting method for the non-alcoholic fatty liver disease that can be conveniently used for medical examination are established. The use of a combination of a kininogen-based marker and a C4-based marker (the full length sequence or partial peptides thereof) enables identification of chronic hepatitis and an asymptomatic virus carrier, as well as non-alcoholic fatty liver disease. | 2011-06-02 |
20110129860 | CALPROTECTIN AND HEMOGLOBIN/HAPTOGLOBIN COMPLEX FROM STOOL SAMPLE TO ASSESS COLORECTAL CANCER - The present invention relates to a method aiding in the assessment of colorectal cancer. The method especially is used in assessing the absence or presence of colorectal cancer in vitro. The method is for example practiced by analyzing biochemical markers, comprising measuring in a stool sample the concentration of the hemoglobin/haptoglobin complex and calprotectin and correlating the concentrations determined to the absence or presence of colorectal cancer. To further improve the assessment of colorectal cancer based on a method of this invention the level of one or more additional marker may be determined together with the hemoglobin/haptoglobin complex and calprotectin in a stool sample and be correlated to the absence or presence of colorectal cancer. The invention also relates to the use of a marker panel comprising the hemoglobin/haptoglobin complex and calprotectin in the early diagnosis of colorectal cancer and it teaches a kit for performing the method of the invention. | 2011-06-02 |
20110129861 | MEASUREMENT OF ANTI-CCP AND SERUM AMYLOID A METHOD TO ASSESS RHEUMATOID ARTHRITIS - The present invention relates to a method aiding in the assessment of rheumatoid arthritis. The method especially is used in assessing the absence or presence of rheumatoid arthritis in vitro. It can be best practiced by analyzing biochemical markers, comprising measuring in a sample the concentration of anti-CCP and serum amyloid A and correlating the concentrations determined to the absence or presence of rheumatoid arthritis. To further improve the assessment of RA in a method of this invention the level of one or more additional marker may be determined together with anti-CCP and serum amyloid A and be correlated to the absence or presence of RA. The invention also relates to the use of a marker panel comprising anti-CCP and serum amyloid A in the diagnosis of rheumatoid arthritis and it teaches a kit for performing the method of the invention. | 2011-06-02 |
20110129862 | METHOD FOR DETERMINING CAUSE OF THE PROLONGATION OF BLOOD COAGULATION TIME - Provided is a method of accurately and easily determining a cause of the prolongation of blood coagulation time. | 2011-06-02 |
20110129863 | METHODS AND SYSTEMS FOR PROCESSING SAMPLES ON POROUS SUBSTRATES - Methods and systems for processing samples fixed to a porous substrate generally comprising, a compressor defining one or more fluid isolation areas, a support, for the porous substrate, having an opening corresponding to one or more of the fluid isolation areas of the compressor, an actuator that causes at least a portion of the compressor to press against the porous substrate, a fluid inlet having access to the fluid isolation area at least when the compressor is pressed against the porous substrate, and a fluid outlet to receive fluid, through the opening in the support corresponding to the fluid isolation area of the compressor, at least when the compressor is pressed against the porous substrate. | 2011-06-02 |
20110129864 | METHOD FOR ANALYZING LYMPH NODE ASPIRATE USING MULTI-ANGLE LIGHT SCATTER FLOW CYTOMETER - Disclosed is a method for identifying lymphatic disease and disease states in mammals. The method uses a multi-angle light scatter flow cytometer to diagnose and treat mammals. The method includes collecting lymph node aspirate from a mammal; scanning the lymph node aspirate in a flow cytometer to generate a diagnostic scan; comparing the diagnostic scan to a known normal scan; identifying differences between the diagnostic scan and the known normal scan; and identifying similarities between the diagnostic scan and known disease scans to identify a cause of lymphadenopathy. Graphical representations of leukocyte identification are generated as a result of the scanning process. By using the graphs, a veterinarian or technician is able to diagnose the effectiveness or ineffectiveness of the treatment. | 2011-06-02 |
20110129865 | METHOD FOR MARKING MATERIALS - The invention relates to a marking system for marking objects wherein said system comprises a microparticle comprising a cross-linked polymer and a marker component wherein the release of said marker component is triggered by contact of the microparticle with an external stimulus and wherein said polymer is a carbohydrate or a protein. | 2011-06-02 |
20110129866 | NOVEL TISSUE CULTURE PLATFORM FOR SCREENING OF POTENTIAL BONE REMODELING AGENTS - The present disclosure provides methods for identifying candidate compounds having bone anti-resorption activity or bone pro-formation activity. The methods involve the use of ex vivo-derived mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts and osteoclast precursors under randomized gravity vector conditions in the presence of the candidate compound. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system. | 2011-06-02 |
20110129867 | Compound Screening Using Chondrocytes - This invention provides a system for obtaining cells of the chondrocyte lineage by differentiating primate pluripotent stem cells. The process involves culturing the cells as a micromass or other aggregate form in a cocktail of differentiation agents that facilitates outgrowth of the desired cell type. Progeny are capable of synthesizing Type II collagen or aggrecan, or other products that are characteristic of the chondrocyte lineage. Chondrocytes and chondrocyte precursor cells obtained according to this disclosure are suitable for use in both research and clinical therapy. | 2011-06-02 |
20110129868 | CELL CAPABLE OF REPLICATING NOVEL HCV REPLICON, CELL CAPABLE OF REPLICATING FULL-LENGTH HCV RNA, AND USE OF THOSE CELLS - According to the present invention, an HCV replicon-replicating cell is produced by a production method including a step of introducing RNA containing an HCV replicon sequence and a selectable marker gene sequence into a Li23 cell or a cured cell derived from a Li23 cell. Further, a full-length HCV RNA-replicating cell is produced by a production method including a step of introducing RNA containing a full-length HCV genome sequence and a selectable marker gene sequence into a Li23 cell or a cured cell derived from a Li23 cell. The use of these cells enables the construction of an HCV life cycle reproduction system that is derived from a cell line other than the HuH-7 cell line and that has capabilities equivalent to those of an HCV life cycle reproduction system derived from the HuH-7 cell line. | 2011-06-02 |
20110129869 | SELECTIVE ENRICHMENT MEDIUM FOR CARBAPENEM-RESISTANT BACTERIA - The present invention relates to a method for direct detection and differentiation of carbapenem-resistant bacteria in a sample comprising (i) inoculation with said sample of a culture medium comprising at least meropenem and/or ertapenem and at least one chromogenic agent, (ii) incubation of said culture medium under conditions allowing the growth of carbapenem-resistant bacteria, and (iii) detection of colonies formed on said culture medium corresponding to carbapenem-resistant bacteria, as well as a culture medium suitable for use in such a method. | 2011-06-02 |
20110129870 | Microbial Screen Test - A method for testing a microbial population including a negative screen includes inoculating a plurality of test ampoules with respective samples and recording a time of the inoculation ( | 2011-06-02 |
20110129871 | CULTURE MEDIUM ENABLING STAPHYLOCOCCUS AUREUS TO BE DIFFERENTIATED FROM COAGULASE-NEGATIVE STAPHYLOCOCCI - A specific culture medium for growth, detection, identification and/or counting of | 2011-06-02 |
20110129872 | METHOD FOR A PRODUCTION OF A RECOMBINANT PROTEIN USING YEAST CO-EXPRESSION SYSTEM - The present invention relates to a method for mass production of a recombinant protein comprising the step of culturing a yeast transformed with: a recombinant gene construct comprising a yeast promoter, a gene coding a signal sequence and a gene coding a target protein; and also with one or more genes coding folding accessory protein selected from the group consisting of PDI1 (protein disulfide isomerase 1), SEC23 (secretory 23), TRX2 (thioredoxin 2) AHA1 (activator of heat shock protein 90 ATPase), and SCJ1 ( | 2011-06-02 |
20110129873 | Recombinant DNA Vectors for Expression of Human Prolactin Antagonists - Embodiments of the present invention relate generally to methods and compositions for producing human prolactin antagonists. Embodiments of the present invention also relate to various methods for improved production of human prolactin antagonists by microorganisms. These microorganisms, including | 2011-06-02 |
20110129874 | Pichia Pastoris Das Promoter Variants - The present invention relates to promoter variants in the form of an isolated polynucleotide comprising: i) a nucleotide sequence consisting of the DAS promoter sequence from | 2011-06-02 |
20110129875 | IMMUNOSUPPRRESSIVE POLYPEPTIDES AND NUCLEIC ACIDS - The invention provides immunosuppressive polypeptides and nucleic acids encoding such polypeptides. In one aspect, the invention provides mutant CTLA-4 polypeptides and nucleic acids encoding mutant CTLA-4 polypeptides. Compositions and methods for utilizing such polypeptides and nucleic acids are also provided. | 2011-06-02 |
20110129876 | Constructs and Methods for the Production and Secretion of Polypeptides - Described herein are molecules, constructs and methods for the production and secretion of polypeptides of interest by host cells, preferably bacterial host cells, and more particularly gram positive bacteria. In particular, the present invention is related to a polynucleic acid encoding a fusion protein and to uses thereof for the secretion of heterologous or homologous polypeptides of interest by a bacterial host cell, preferably | 2011-06-02 |
20110129877 | PULLULANASE VARIANTS AND METHODS FOR PREPARING SUCH VARIANTS WITH PREDETERMINED PROPERTIES - The present invention relates to pullulanase variants, wherein the variants have improved properties, for example, altered pH optimum, improved thermostability, altered substrate specificity, increased specific activity or altered cleavage pattern. | 2011-06-02 |
20110129878 | BIOREACTIVE AGENTS - This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances. | 2011-06-02 |
20110129879 | Hybrid Genes and Enzymes of Glucanase and Dextransucrase and Processes for Preparing Isomalto-Oligosaccharides or Dextran Using the Same - Disclosed are hybrid genes of glucanase and dextransucrase, recombinant vectors comprising said hybrid genes, microorganisms which are transformed with said recombinant vectors, hybrid enzymes which are expressed from said hybrid genes, and processes for preparing isomalto-oligosaccharides or dextran using said microorganisms or enzymes. Expensive isomalto-oligosaccharides and low molecular weight dextran for clinical use can be produced simply and effectively from cheap substrate-sucrose, using a single bacterial strain or enzyme. | 2011-06-02 |
20110129880 | PROCESS FOR TREATING BIOMASS TO INCREASE ACCESSIBILITY OF POLYSACCARIDES CONTAINED THEREIN TO HYDROLYSIS AND SUBSEQUENT FERMENTATION, AND POLYSACCHARIDES WITH INCREASED ACCESSIBILITY - In this invention, a process for producing fermentable sugars derivable from biomass that contains polysaccharide, such as cellulose, which has been made increasingly accessible as a substrate for enzymatic degradation or other methods of depolymerization. The process of the present invention increases accessibility of polysaccharides, typically present in biomass and produces polysaccharides with increased accessibility. The polysaccharides with increased accessibility may be subsequently saccharified to yield fermentable sugars. These fermentable sugars are subsequently able to be fermented to produce various target chemicals, such as alcohols, aldehydes, ketones or acids. | 2011-06-02 |
20110129881 | RECOMBINANT BETA-GLUCOSIDASE VARIANTS FOR PRODUCTION OF SOLUBLE SUGARS FROM CELLULOSIC BIOMASS - The invention relates to recombinant expression of a variant form of a fungal C1 strain β-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the β-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant β-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel. | 2011-06-02 |
20110129882 | GENE CODING FOR GLUCOSE-6-PHOSPHATE-DEHYDROGENASE PROTEINS - The present invention relates to isolated nucleic acid molecules encoding mutants of glucose-6-phosphate dehydrogenase, and vectors and hosts cells including such nucleic acid molecules. These nucleic acid molecules are involved in the biosynthesis of a fine chemical, e.g., an amino acid such as lysine. The present invention also relates to methods of producing and modulating the production of fine chemicals, e.g., lysine, by culturing recombinant microorganisms containing these nucleic acid molecules under conditions such that the fine chemical is produced. | 2011-06-02 |
20110129883 | INACTIVATION OF GENES OF THE MEP PATHWAY - The invention relates to cells and organisms as well as to methods for producing said cells and organisms, according to which intermediates of the mevalonate-independent pathway for isoprenoid biosynthesis (MEP pathway) are enriched by deleting or inactivating genes. The derivatives can also be enriched by using enzyme inhibitors. The enriched intermediates may be used as substrates in enzyme activity tests. The inventive cells and organisms and the enriched intermediates can further be used in the production of medicaments. | 2011-06-02 |
20110129884 | METHOD FOR THE CULTIVATION OF MICROORGANISMS OF THE ORDER THRAUSTOCHYTRIALES - The present invention relates generally to the field of microorganism cell culture. It devises a cell culture medium for and a method of culturing microorganisms of the order Thraustochytriales, especially for the production of omega-3-fatty acids. Furthermore it devises the use of ammonium tartrate as nitrogen source for the cultivation of micro-organisms of the order Thraustochytriales. | 2011-06-02 |
20110129885 | MICROORGANISM FOR THE PRODUCTION OF SUCCINIC ACID - The invention relates to an isolated genetically modified microorganism in which the gene IDH1 and at least one of the genes SDH2 and DIC1 are under the control of a first promoter that is repressed to a growth culture medium by means of a cultivation additive and is active in the absence of the cultivation additive. The genes that are part of the group comprising “PYC1, ACS1, CIT1, ACO1, ICL1, MSL1, and CIT2, optionally also MDH3” are constitutively active. The invention further relates to uses of such a microorganism, especially for producing succinic acid. | 2011-06-02 |
20110129886 | Biorefinery Process for Extraction, Separation, and Recovery of Fermentable Saccharides, other Useful Compounds, and Yield of Improved Lignocellulosic Material from Plant Biomass - Non-food plant biomass is subjected to hot-water extraction in a pressurized vessel at an elevated temperature up to about 250° C. and at a pH below about 7.0, to yield an aqueous extract containing hemicellulosic components, other wood-derived compounds, and a lignocellulosic residue. The separated aqueous extract or liquor is purified and concentrated through a multi-step process producing fermentable sugars. At each stage, inhibitory chemicals such as acetic acid, lignin, and furfural are separated and eventually recovered as commercial chemicals. The lignocellulosic residue may be further processed, as a material with enhanced resistance to sorption of water, for manufacture of improved pulp and paper, construction materials, pellet fuel, and/or other useful products. | 2011-06-02 |
20110129887 | IMMOBILIZED PRODUCT TOLERANT MICROORGANISMS - Methods for adapting or selecting microorganisms with increased product tolerance are provided Additionally, a bioreactor capable of operation in either packed bed or fluidized bed is disclosed along with methods to use the bioreactor for culturing microorganisms adapted or selected increased product tolerance | 2011-06-02 |
20110129888 | Method for Fermenting Cellulosics - The present invention is directed to host cells capable of fermenting cellulosic materials for the production of ethanol. Microorganisms engineered to be able to use amorphous cellulosic materials in a fermentation process to produce ethanol are disclosed. Additionally, methods of using the host organisms of the invention and compositions for producing ethanol according to the invention are disclosed. | 2011-06-02 |
20110129889 | Process for Production of Ethanol from Lignocellulosic Material - This invention relates to a process for production of ethanol from lignocellulosic material. Lignocellulosic material treated with a dicarboxylic acid, preferably with oxalic acid, separating a hemicellulosic fraction to ferment pentose sugar. The lignin is dissolved in alkali, preferably with NaOH, separating a cellulose fraction for further enzymatic treatment with one or more than one cellulytic enzymes capable of hydrolyzing cellulose. The enzyme hydrolyasate is further subjected to fermentation in the presence of ethanol-producing yeast, preferably | 2011-06-02 |
20110129890 | METHOD OF TREATING SUBSTANCE CONTAINING LIGNOCELLULOSE OR CELLULOSE - A method for converting a material comprising lignocellulose or cellulose into a substance convertible with yeast into ethyl alcohol, wherein a mixture comprising 1 part by weight of the material and 0.5 to 5 parts by weight of water is stirred at a temperature of 150 to 270 degrees C. in a vessel closed in terms of pressure in conditions of providing a high shearing force to be pulverized to an average of maximum dimensions of 1 to 20 micrometers, whereby the material is degraded and at least 15% by weight of the cellulose in the material is converted into a substance convertible into ethyl alcohol. It is possible to prepare saccharides for alcohol fermentation from lignocellulose or cellulose by using this method. | 2011-06-02 |
20110129891 | Process for Controlled Homogeneous Acid Leaching - A method for leaching a material containing one or more target metals using an acidic leaching solution to extract said one or more target metals, said method including (I) empirically determining an optimal acid concentration range for said acidic leaching solution by: (a) determining the relationship between the concentration of extracted target metal/s and acid consumption in said leaching solution, (b) utilizing said relationship to evaluate value parameters for the target metal containing material as a function of said acid consumption, and (c) determining said optimal acid concentration range, which is the pH range corresponding to an optimal value parameter; and (II) controlling the concentration of said acidic leaching solution such that its pH is substantially within the optimal acid concentration range throughout said material. | 2011-06-02 |
20110129892 | PRODUCTION OF CELL TISSUE HAVING THREE-DIMENSIONAL STRUCTURE USING ELECTROSTATIC INK JET PHENOMENON - According to the present invention, a method and an apparatus for producing a cell tissue of blood vessels or the like with the use of an electrostatic ink jet phenomenon are provided. | 2011-06-02 |
20110129893 | MONITORING TARGET ENDOGENOUS SPECIES - An electrode comprising: a conducting substrate for detecting an electroactive agent; a catalytic agent held on the substrate for converting a non-electroactive agent to an electroactive agent by a catalytic process which consumes oxygen; and an oxygen reservoir, for releasing oxygen to feed the catalytic process, held within a polymer matrix on the substrate. The electrode allows for detection of materials such as glucose when using oxidase enzymes. The invention solves the problem of fluctuation or depletion of oxygen in the environment in which the measurements are being taken. | 2011-06-02 |
20110129894 | SECRETION OPTIMIZED MICROORGANISM - Proteins having a cofactor can be secreted in an improved manner in a microorganism belonging to the genus | 2011-06-02 |
20110129895 | Method For Purifying Protein - A method is provided for purifying protein having steps of loading a sample containing proteins into a column containing an ion exchanger in a first direction to allow the ion exchanger to absorb proteins; and passing a salt solution through the column in a second direction opposite to the first direction to elute a target protein from the ion exchanger. The method is proven as capable of improving homogeneity of eluted target protein without combining minute and complicated techniques for protein purification. Therefore, the method is convenient, efficient and economic for purifying proteins. | 2011-06-02 |
20110129896 | INHIBITORS OF GSK-3 AND CRYSTAL STRUCTURES OF GSK-3Beta PROTEIN AND PROTEIN COMPLEXES - The present invention relates to inhibitors of GSK-3 and methods for producing these inhibitors. The invention also provides pharmaceutical compositions comprising the inhibitors and methods of utilizing those compositions in the treatment and prevention of various disorders, such as diabetes and Alzheimer's disease. In addition, the invention relates to molecules or molecular complexes which comprise binding pockets of GSK-3β or its homologues. The invention relates to a computer comprising a data storage medium encoded with the structure coordinates of such binding pockets. The invention also relates to methods of using the structure coordinates to solve the structure of homologous proteins or protein complexes. The invention relates to methods of using the structure coordinates to screen for and design compounds that bind to GSK-3β protein or homologues thereof. The invention also relates to crystallizable compositions and crystals comprising GSK-3β protein or GSK-3β protein complexes. | 2011-06-02 |
20110129897 | PROCESS FOR MAKING MICRO-SIZED PROTEIN PARTICLES - A process of making micro-sized protein particles comprising the step of drying nano-sized protein particles suspended in a liquid medium under conditions to agglomerate the nano-sized protein particles and thereby form micro-sized protein particles. | 2011-06-02 |
20110129898 | Methods and compositions for increasing nuclease activity - Disclosed herein are methods and compositions for increasing nuclease activity to increase nuclease-mediated genomic modifications. | 2011-06-02 |
20110129899 | MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL, 4-HYDROXYBUTANAL, 4-HYDROXYBUTYRYL-COA, PUTRESCINE AND RELATED COMPOUNDS, AND METHODS RELATED THERETO - The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine. | 2011-06-02 |
20110129900 | LOW OXYGEN BIOLOGICALLY MEDIATED NUTRIENT REMOVAL - The present invention is directed to a substantially odorless biologically mediated treatment process for solid and liquid organic wastes. The present invention also provides for a novel nutrient rich humus material produced from the biologically mediated treatment process. The bioconversion process of the present invention results from low electron acceptor concentrations and high quantities of microorganisms in a diverse microbial community. | 2011-06-02 |
20110129901 | USE OF A CARBONACEOUS SUBSTITUTE FOR THE PRODUCTION OF YEAST - The present invention relates to novel strains of | 2011-06-02 |
20110129902 | Microbial strain Alteromonas SP. SN2 for degrading polycyclic aromatic hydrocarbon - The present invention relates to a novel microbial strain, | 2011-06-02 |
20110129903 | Method of making an artificial micro-gland using taxis - A method is used for making an artificial micro-gland by taxis. A monodisperse multiple emulsion is produced with a first fluid; a second fluid confined within the first fluid; a third fluid within the second fluid. Interfaces between the fluids permit living cells dispersed in the one of the fluids to migrate towards an adjacent fluid having a different concentration of an agent affecting the metabolic activity of the living cells. Waiting, usually about 30 minutes, allows the living cells to migrate to the interface, forming the continuous membrane. Once formed, the artificial micro-gland is removed from the remains of the emulsion. The artificial micro-gland may also be given a second layer of different cells when the emission of the cells of the artificial micro-gland is used as the agent to attract the different cells. The method may also be used to produce an artificial micro-gland within an artificial micro-gland. | 2011-06-02 |
20110129904 | METHODS AND ORGANISMS FOR CONVERTING SYNTHESIS GAS OR OTHER GASEOUS CARBON SOURCES AND METHANOL TO 1,3-BUTANEDIOL - A non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway includes at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme or protein expressed in a sufficient amount to produce 1,3-BDO. A method for producing 1,3-BDO that includes culturing the this non-naturally occurring microbial organism under conditions and for a sufficient period of time to produce 1,3-BDO. | 2011-06-02 |
20110129905 | MODIFIED PICHIA STRAIN - The present invention generally relates to a modified strain of yeast. More specifically, it relates to a strain of | 2011-06-02 |
20110129906 | PHOTOBIOREACTOR, SYSTEM AND METHOD FOR THE CULTIVATION OF PHOTOSYNTHETIC MICROORGANISMS - The present invention relates to the cultivation of photosynthetic microorganisms, and more particularly, to a low-cost flexible photobioreactor ( | 2011-06-02 |
20110129907 | Method of conversion of biodegradable, hygienically non-stabilized substrate into hygienically stabilized product - A method for treating hygienically unstable waste is disclosed. Waste is allowed to aerobically ferment for approximately 36 hours at a temperature of 40 to 50° C. During aerobic fermentation, an external air supply maintains the appropriate temperature, while restacking of the substrate ensures uniform oxygen distribution. After approximately 36 hours of aerobic fermentation, the amount of externally supplied air is decreased, leading to a gradual temperature rise. The substrate is kept at a temperature range of 70 to 80° C. for a period of 30 to 60 minutes, followed by intensive aeration which is performed until the maximum moisture content of 10% is reached. The waste has then been converted to a hygienically stabilized product. | 2011-06-02 |
20110129908 | Apparatus and method for maintaining and/or restoring viability of organs - An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The method includes perfusing the organ at hypothermic and/or normothermic temperatures, preferably after hypothermic organ flushing for organ transport and/or storage. The method can be practiced with prior or subsequent static or perfusion hypothermic exposure of the organ. Organ viability is restored by restoring high energy nucleotide (e.g., ATP) levels by perfusing the organ with a medical fluid, such as an oxygenated cross-linked hemoglobin-based bicarbonate medical fluid, at normothermic temperatures. In perfusion, organ perfusion pressure is preferably controlled in response to a sensor disposed in an end of tubing placed in the organ, by a pneumatically pressurized medical fluid reservoir, providing perfusion pressure fine tuning, overpressurization prevention and emergency flow cut-off. In the hypothermic mode, the organ is perfused with a medical fluid, preferably a simple crystalloid solution containing antioxidants, intermittently or in slow continuous flow. The medical fluid may be fed into the organ from an intermediary tank having a low pressure head to avoid organ overpressurization. Preventing overpressurization prevents or reduces damage to vascular endothelial lining and to organ tissue in general. Viability of the organ may be automatically monitored, preferably by monitoring characteristics of the medical fluid perfusate. The perfusion process can be automatically controlled using a control program. | 2011-06-02 |
20110129909 | LIQUID SAMPLE ANALYSIS DEVICE - A liquid sample analysis device includes a holder part | 2011-06-02 |
20110129910 | COOLING DEVICE, AND ASSEMBLY, AND METHODS FOR LOWERING TEMPERATURE IN A CHEMICAL REACTION - Embodiments provide a cooling device. The cooling device comprises a container configured as a heat sink. The container is at least partially made from heat conducting material. The cooling device further comprises endothermic chemical material which is contained in the container. | 2011-06-02 |
20110129911 | Bioreactor Chamber - Embodiments of the invention provide a chamber for a bioreactor, the chamber having a fluid inlet aperture and a fluid outlet aperture disposed at respective different locations of a wall of the chamber, with respect to a normal upright orientation of the chamber the chamber being provided with an upper wall portion defining an upper boundary of the chamber. The upper wall portion has an internal surface having a first portion that is vertically displaced with respect to a second portion. The internal surface of the upper wall portion is arranged to promote expulsion of trapped gas bubbles through the outlet aperture, the first and second portions of the upper wall portion each comprising a sloped portion. | 2011-06-02 |
20110129912 | SELECTIVE CONFORMATION OF CELL CULTURING SUPPORT LAYER - Embodiments described herein relate to in vitro cell culture. Embodiments include a substrate having a support layer that includes support molecules having a first region configured to bind to the substrate and having a photo-responsive region configured to change conformation between at least two conformations. In a first conformation of the photo-responsive region, the support molecules are configured to be more accessible to cells. In a second conformation of the photo-responsive region, the support molecules are configured to be less accessible to cells. | 2011-06-02 |
20110129913 | Device for cell culture - The device for cell culture comprises: a container body ( | 2011-06-02 |
20110129914 | THERMOCYCLING OF A BLOCK COMPRISING MULTIPLE SAMPLE - The present invention relates to the field of high throughput analysis of samples. In particular, the present invention is directed to a device, a system and a method for simultaneous tempering of multiple samples. More particular, the invention relates to the simultaneous thermocycling of multiple samples to perform PCR in a microtiter plate format. | 2011-06-02 |
20110129915 | APPARATUS AND METHOD FOR INCUBATING CELL CULTURES - A portable, self-contained incubation apparatus has a cell container in which a cell culture can grow. The cell container resides on a base along with other components that maintain the environmental conditions inside of the container within a desired range. The self-contained incubation apparatus is portable allowing the conditions within the cell container to be precisely controlled even as it is being moved from one location to another. Further, at least one transparent surface of the cell container enables observation of the cell culture. Thus, the culture can be observed while the environmental conditions within the container are being controlled by components of the incubation apparatus. Since the cells can be observed without breaking the air-tight seal of the container, observation of the cells can be performed as often as is desired without introducing contaminants to the culture or otherwise significantly affecting the growth environment within the container. Indeed, observation of the cells can be performed as often as is desired without introducing contaminants to the culture or otherwise significantly affecting the growth environment within the container. | 2011-06-02 |
20110129916 | MONOCLONAL ANTIBODIES WITH SPECIFICITY FOR FETAL ERYTHROID CELLS - The present invention concerns a monoclonal antibody and corresponding hybridoma cells and antigens suitable for isolating fetal cells from maternal blood. The inventive monoclonal antibody reacts with a surface antigen present on fetal red blood cells including their nucleated precursor cells, but not with surface antigens on adult erythroid cell. | 2011-06-02 |
20110129917 | CELL ISOLATION METHOD - The present invention is a method for isolating a desired cell, which comprises selectively applying culture conditions including a culture medium to a sample potentially containing various cells, with addition of a cell enlarger simultaneously with, before or after the application. Thereby, it is possible to conveniently and efficiently obtain unknown but useful microorganisms occurring in a natural environment that are less competitive. | 2011-06-02 |
20110129918 | METHOD FOR EXPANDING MESENCHYMAL STEM CELLS IN LOW-DENSITY AND HYPOXIC CULTURE - The present invention relates to a novel method for expanding mesenchymal stem cells (MSCs) in low-density and hypoxic condition as compared to normal air conditions traditionally used in cell culture. The present method provides rapid and efficient expansion of human MSCs without losing cellular proliferation and stem cell properties, including increase in proliferation, decrease in senescence, and increase in differentiation potential both in vitro and in vivo. The expanded MSCs by the present method may maintain normal karyotyping, and will not form tumor when transplanted into mamma. | 2011-06-02 |
20110129919 | Microcarriers for Stem Cell Culture - We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells. | 2011-06-02 |
20110129920 | BIOMARKERS FOR ALZHEIMER'S DISEASE - The present invention provides protein-based biomarkers and biomarker combinations that are useful in qualifying Alzheimer's disease status in a patient. In particular, the biomarkers of this invention are useful to classify a subject sample as Alzheimer's or non-Alzheimer's dementia or normal. The biomarkers can be detected by SELDI mass spectrometry. In addition, the invention provides appropriate treatment interventions and methods for measuring response to treatment. Certain biomarkers of the invention may also be suitable for employment as radio-labeled ligands in non-invasive imaging techniques such as Positron Emission Tomography (PET). | 2011-06-02 |
20110129921 | TARGETED POLYMER BIOCONJUGATES - Polymer bioconjugate having a RNAi agent covalently coupled to the alpha or omega end of a pH-dependent membrane-destabilizing polymer. | 2011-06-02 |
20110129922 | METHOD FOR INDUCING DIFFERENTIATION INTO CARDIOMYOCYTES USING G-CSF - A method for inducing differentiation of ES cells into cardiomyocytes, which comprises contacting the ES cells with an agonist for G-CSF receptor. | 2011-06-02 |
20110129923 | CELL CULTURE METHODS AND DEVICES UTILIZING GAS PERMEABLE MATERIALS - Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency. | 2011-06-02 |
20110129924 | Porous Polymeric Articles - Porous polymeric articles, and more specifically, porous polymeric articles for tissue engineering and organ replacement, are described. In some embodiments, methods described herein include use of a polymer-solvent system (e.g., phase inversion) to generate porosity in a structure. The process may include formation of a structure precursor material including a first crosslinkable component and a second component that can be precipitated in a precipitation medium. The structure precursor material may be shaped into a three-dimensional shape by a suitable technique such as three-dimensional printing. Upon shaping of the structure precursor material, at least a portion of the first component may be crosslinked. The structure may then be contacted with a precipitation medium to remove the precursor solvent from the structure, which can cause the second polymer component to precipitate and form a porous structure containing a network of uniform pores. In some embodiments, the porous structure is constructed and arranged for use as a template for ultrafiltration, cell growth, and/or for forming complex, biomimetic, porous biohybrid organs, where living cells can be immobilized and perform their normal physiological functions. | 2011-06-02 |
20110129925 | ZEOLITE AND BONE MIMETIC ZEOLITE BASED COATINGS FOR BIOIMPLANTS - The disclosure provides biocompatible metal compositions, methods of making such compositions and uses thereof, including a method of synthesizing zeolite coatings. The disclosure further provides the zeolite-hydroxyapatite composite coatings and methods of making them, which includes forming a base zeolite layer, forming a hydroxyapatite layer on the base zeolite layer, and interlocking the hydroxyapatite layer with an outer zeolite layer. The composite can be formed on a metal substrate for bioimplants, such as titanium alloy and/or stainless steel, which is used for bioimplants. | 2011-06-02 |
20110129926 | DRY POWDER CELL CULTURE PRODUCTS AND METHODS OF PRODUCTION THEREOF - The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations. | 2011-06-02 |
20110129927 | CHINESE HAMSTER APOPTOSIS-RELATED GENES - Provided is an isolated polypeptide comprising a | 2011-06-02 |
20110129928 | Method of Manufacturing Induced Pluripotent Stem Cell Originated from Somatic Cell - Disclosed is a method for manufacturing stem cells including preparing Oct-4 gene, Sox2 gene, C-myc gene, and Klf-4 gene from mouse embryonic stem cells, and allowing each of the genes to be infected in host cells using a lentiviral vector system to generate viruses in which each of the genes are induced; concentrating or mixing each of the viruses to prepare a virus concentrated mixture, and mixing the virus concentrated mixture and a first culture solution to prepare a virus solution; floating mouse somatic cells having been cultivated in advance in a first culture dish, and mixing and reacting the floated somatic cells and the virus solution to prepare a somatic cell-virus mixture; adding and retaining the somatic cell-virus mixture as is in a second culture dish including a second culture solution to induce the genes in the somatic cells; and cultivating the somatic cells. | 2011-06-02 |
20110129929 | APPARATUS AND METHOD FOR DETERMINING THE RESULTS OF ASSAYS - The present invention provides a method for the real-time continuous monitoring of a change or density and/or viscosity within a test sample. Such methods can be used to determine the occurrence of a chemical reaction within a test sample where the same causes and increase or decrease in the density and/or viscosity of the sample due to, for example, a gelation, precipitation or coagulation occurring within the test sample. There is further provided a multi-resonator apparatus for use in measuring the density and/or viscosity of a test sample in which the multi-beam resonator is immersed. | 2011-06-02 |
20110129930 | INSPECTION METHOD AND APPARATUS - In an embodiment, there is disclosed an inspection method for detecting the presence of imprintable medium on an imprint lithography template. The method includes contacting the imprint lithography template with a marker, the marker being attachable to imprintable medium that may be on the imprint lithography template, the marker being configured to interact with incident radiation when attached to the imprintable medium, directing radiation at the imprint lithography template, and measuring radiation re-directed by the imprint lithography template to attempt to detect presence of a marker that has attached to the imprintable medium, from the interaction of the marker with the incident radiation, and thus detect the presence of imprintable medium to which the marker is attached. | 2011-06-02 |
20110129931 | MICROFLUIDIC SYSTEM FOR DETECTING A BIOLOGICAL ENTITY IN A SAMPLE - According to various embodiments, a microfluidic system for detecting a biological entity in a sample volume is provided. The microfluidic system may include: a chamber configured to receive the sample volume, wherein the chamber includes a detection region for detecting the biological entity; a first port in fluid communication with the chamber; and a second port including a filter in fluid communication with the chamber; and wherein a fluid provided to the first port or the second port flows between the first port and the second port through the chamber. | 2011-06-02 |
20110129932 | Lipoprotein Assay - The present invention concerns a method of determining the concentration of total lipoprotein in a sample. The method involves the steps of: (i) adding to an aliquot of the sample a lipophilic dye that binds to lipoproteins in the sample and which when so bound fluoresces under appropriate excitation; and (ii) determining the total lipoprotein concentration in the sample using fluorescence analysis. A method of analysing the lipoprotein content of a sample solution using a dye that discriminates between different types of lipoprotein is also disclosed. | 2011-06-02 |
20110129933 | MEANS AND METHODS FOR ASSESSING INCREASED PEROXISOMAL PROLIFERATION - The present invention pertains to the field of toxicological as for risk stratification of chemical compounds. Specifically, it relates to methods for (i) diagnosing, (ii) determining whether a compound is capable of inducing, (iii) identifying a drug for treating increased peroxisomal proliferation. Furthermore, the present invention relates to a data collection comprising characteristic values of at least five metabolites, a data storage medium comprising said data collection, and a system and a device for diagnosis. Finally, the present invention pertains to the use of a group of metabolites or means for the determination thereof for the manufacture of diagnostic devices or compositions. The metabolite markers of the metabolome are selected from: Coenzyme Q10, 16-Methylheptadecanoic acid, 17-Methyloctadecanoic acid, Eicosatrienoic acid (C20:3), Threonine, Proline, Tyrosine, trans-4-Hydroxyprolme, Pantothenic acid, Coenzyme Q9, Glycerol, Palmitic acid (C16:0), Lmoleic acid (C18:cis (9, 12) 2), 14-Methylhexadecanoic acid, gamma-Linoleic acid (C18:cis (6, 9, 12) 3), Threonic acid, Cytosine, Phosphatidyl-choline (C18:0/C22:6). Different multimarker sets are proposed for male and female subjects. | 2011-06-02 |
20110129934 | METHOD FOR INFERRING TEMPERATURE IN AN ENCLOSED VOLUME - A method for inferring temperature in an enclosed volume containing a fuel/oxidant mixture, the method comprises placing at least one wire in the enclosed volume. The at least one wire having an identifiable property wherein the identifiable property of the at least one wire changes from a first identifiable state at a temperature below the auto-ignition temperature of the fuel/oxidant mixture to a second identifiable state at a temperature above the auto-ignition temperature of the fuel/oxidant mixture, and determining if the identifiable property of the at least one wire has changed from the first identifiable state to the second identifiable state and hence if the temperature in the enclosed volume is above the auto-ignition temperature of the fuel/oxidant mixture. | 2011-06-02 |
20110129935 | PROTEIN STABILITY ASSAY USING A FLUORESCENT REPORTER OF PROTEIN FOLDING - The invention relates to methods and compositions for assessing protein stability, including improved assays for distinguishing between soluble and aggregated proteins. The methods and compositions include measuring residual fluorescence of a fusion protein in a soluble fraction as an indicator of protein solubility, and monitoring fluorescence quenching of a fusion protein as an indicator of protein stability. The fusion protein may comprise an amino acid sequence of a protein of interest, a peptide linker amino acid sequence and an amino acid sequence of a fluorescent marker protein. | 2011-06-02 |
20110129936 | D-DIMER, TROPONIN, AND NT-PROBNP FOR PULMONARY EMBOLISM - The present invention relates to a method of diagnosing acute pulmonary embolism (PE) in a subject including a) determining the amount of fibrin-fibrinogen degradation products, in particular D-dimer in a sample of the subject; b) determining the amount of a natriuretic peptide in a sample of the subject; c) determining the amount of a cardiac troponin in a sample of the subject; and d) comparing the amounts determined in steps a) to c) to reference amounts, thereby establishing the diagnosis. Included is also a method of deciding on a therapy of a subject diagnosed with PE and a method of monitoring the therapy. | 2011-06-02 |
20110129937 | SEMICONDUCTOR DETECTOR FOR PEROXIDE-BASED EXPLOSIVES - A device for the detection of a peroxide-based explosive, in particular, triacetone triperoxide (TATP), which is based on a molecular controlled semiconductor resistor (MOCSER) and composed of at least one insulating or semi-insulating layer, at least one conducting semiconductor layer, two conducting pads and a layer of multifunctional organic molecules capable of adsorbing molecules of said peroxide-based explosive. Further is provided an array of semiconductor devices for the selective detection of a peroxide-based explosive, as well as a method for the selective detection of vapors of a peroxide-based explosive in a gaseous mixture using said array. The multifunctional organic molecules are from the group of cyclodextrins, thiols or alkylphosphonates with RPO(OH) 2 wherein R is an aliphatic hydrocarbyl with C6-C22. | 2011-06-02 |