| 20th week of 2012 patent applcation highlights part 45 |
| Patent application number | Title | Published |
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| 20120122100 | COMPOSITIONS FOR USE IN IDENTIFICATION OF BACTERIA - The present invention provides compositions, kits and methods for rapid identification and quantification of bacteria by molecular mass and base composition analysis. | 2012-05-17 |
| 20120122101 | COMPOSITIONS FOR USE IN IDENTIFICATION OF BACTERIA - The present invention provides compositions, kits and methods for rapid identification and quantification of bacteria by molecular mass and base composition analysis. | 2012-05-17 |
| 20120122102 | COMPOSITIONS FOR USE IN IDENTIFICATION OF BACTERIA - The present invention provides compositions, kits and methods for rapid identification and quantification of bacteria by molecular mass and base composition analysis. | 2012-05-17 |
| 20120122103 | COMPOSITIONS FOR USE IN IDENTIFICATION OF BACTERIA - The present invention provides compositions, kits and methods for rapid identification and quantification of bacteria by molecular mass and base composition analysis. | 2012-05-17 |
| 20120122104 | Triple-Stranded Nucleobase Structures and Uses Thereof - The present disclosure relates to compositions and methods of using triplex structures generated by a duplex of a polypurine tract and complementary polypyrimidine tract and a triplex-forming nucleobase polymer that hydrogen bonds to both the purine and pyrimidine bases of the polypurine-polypyrimidine duplex. | 2012-05-17 |
| 20120122105 | REAL TIME CLEAVAGE ASSAY - A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured. | 2012-05-17 |
| 20120122106 | Mutation Detection Assay - A method of sample analysis is provided. In certain embodiments, the method involves: a) amplifying a product from a sample that comprises both wild type copies of a genomic locus and mutant copies of the genomic locus that have a point mutation relative to said wild type copies of the genomic locus, to produce an amplified sample, where: i. the amplifying is done using a first primer and a second primer; and ii. the first primer comprises a 3′ terminal nucleotide that base pairs with the point mutation and also comprises a nucleotide sequence that is fully complementary to a sequence in the locus with the exception of a single base mismatch within 6 bases of the 3′ terminal nucleotide; and b) detecting the presence of said product in said amplified sample using a flap assay that employs an invasive oligonucleotide. A kit for performing the method is also provided. | 2012-05-17 |
| 20120122107 | HOMEOSTATIC MULTIDOMAIN PROTEIN, AND USES FOR IT - The invention relates to the discovery and characterization of mannan binding lectin-associated protein (map44), a new protein that acts in the lectin pathway of complement activation. | 2012-05-17 |
| 20120122108 | MICROFLUIDIC CARTRIDGE - The technology described herein generally relates to microfluidic cartridges configured to amplify and detect polynucleotides extracted from multiple biological samples in parallel. The technology includes a microfluidic substrate, comprising: a plurality of sample lanes, wherein each of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another: an inlet; a first valve and a second valve; a first channel leading from the inlet, via the first valve, to a reaction chamber; and a second channel leading from the reaction chamber, via the second valve, to a vent. | 2012-05-17 |
| 20120122109 | NEURAL PROGENITOR CELLS DERIVED FROM EMBRYONIC STEM CELLS - The present invention relates to undifferentiated human embryonic stem cells, methods of cultivation and propagation and production of differentiated cells. In particular it relates to the production of human ES cells capable of yielding somatic differentiated cells in vitro, as well as committed progenitor cells such as neural progenitor cells capable of giving rise to mature somatic cells including neural cells and/or glial cells and uses thereof. This invention provides methods that generate in vitro and in vivo models of controlled differentiation of ES cells towards the neural lineage. The model, and cells that are generated along the pathway of neural differentiation may be used for: the study of the cellular and molecular biology of human neural development, discovery of genes, growth factors, and differentiation factors that play a role in neural differentiation and regeneration, drug discovery and the development of screening assays for teratogenic, toxic and neuroprotective effects. | 2012-05-17 |
| 20120122110 | METHOD FOR ISOLATING CELLS - The present invention relates to a method and kit for the isolation of cells from a sample. The sample is treated with an extraction solution that comprises at least MgCl | 2012-05-17 |
| 20120122111 | RNA SEQUENCE-SPECIFIC MEDIATORS OF RNA INTERFERENCE - The present invention relates to a | 2012-05-17 |
| 20120122112 | ANTIBODIES AS A CANCER DIAGNOSTIC - Method and kits are provided for the detection and diagnosis of metastatic disease. More particularly, the methods and kits employ compounds that can detect EphA2, a specific epithelial cell tyrosine kinase that is overexpressed in metastatic tumor cells. In one embodiment the compound is an antibody capable of binding to an epitope of EphA2. | 2012-05-17 |
| 20120122113 | Signature Of Secreted Protein Isoforms Specific To Ovarian Cancer - The present invention relates to the identification of secreted protein isoforms specific in ovarian cancer and methods for diagnosis or prognosis of ovarian cancer in a subject by detecting the secreted protein isoforms. | 2012-05-17 |
| 20120122114 | IMMUNOASSAY FOR THE DETECTION OF PROCALCITONIN - The present invention relates to an in vitro method for the detection of Procalcitonin or a fragment thereof of at least 20 amino acid residues in length in a biological sample derived from a bodily fluid obtained from a subject, comprising the steps of: (i) contacting said sample with at least two antibodies or functional fragments thereof directed against different epitopes within Procalcitonin, and (ii) qualitatively or quantitatively detecting binding of said at least two antibodies to Procalcitonin or said fragment thereof, wherein binding indicates the presence or concentration of Procalcitonin or said fragment in said sample, wherein at least one antibody or functional fragment thereof is directed against an epitope comprised in the sequence spanning amino acid residues 2 to 52 of Procalcitonin. The invention also pertains to antibodies directed against an N-terminal epitope of Procalcitonin and kits comprising antibodies directed against PCT. | 2012-05-17 |
| 20120122115 | Bacterial quorum sensing biosensor - The present invention generally relates to fluorescent resonance energy transfer protein compounds and methods for using such compounds as biosensors. The present invention also relates to one or more nucleic acids for encoding the protein compounds, vectors containing the nucleic acids, cells transformed by the vectors, and methods for making and using the foregoing compositions. | 2012-05-17 |
| 20120122116 | USE OF LYMPHOCYTES TO MEASURE ANTHRAX LETHAL TOXIN ACTIVITY - It is disclosed herein that isolated lymphocytes, such as human B-cells and CD4 | 2012-05-17 |
| 20120122117 | SECRETED PHOSPHOLIPASE A2 BIOMARKERS FOR ARTHRITIS - The present invention relates to the use of protein expression profiles of sPLA2 isoforms with clinical relevance to osteoarthritis (OA). In particular, the invention provides methods for diagnosing OA or determining risk factors for development of OA based on expression of sPLA2-IIA. | 2012-05-17 |
| 20120122118 | Monclonal antibodies, hybridomas and methods for use - The present invention provides monoclonal antibodies to TM9SF-proteins and hybridoma cell lines that produce the monoclonal antibodies to TM9SF4. This invention also provides a method for determining the level of TM9SF4-protein in biological fluid samples, tissue samples and in microvesicles such as exosomes, comprising kit for determining the level of TM9SF4 protein in human exosomes and other microvesicles, in tissue samples, and in biological fluids. | 2012-05-17 |
| 20120122119 | METHODS OF IDENTIFYING COMPOUNDS THAT BIND TO A TOLL-LIKE RECEPTOR - The present invention is directed to nucleic acid molecules and polypeptides encoding a dsRNA receptor (dsRNA-R). The dsRNA-R contains a THD, interacts with the MyD88 adapter protein, and may bind to dsRNA. The present invention is also directed to antibodies against dsRNA-R and to methods of modulating an immune response and the methods of identifying compounds which bind to and/or modulate dsRNA-R. | 2012-05-17 |
| 20120122120 | ANTIBODIES AGAINST HUMAN EPO RECEPTOR - An antibody binding to human EPO-R, characterized in specifically binding pY461, pY430 or p465 is useful for the determination of activated EPO receptor. | 2012-05-17 |
| 20120122121 | ANTIBODIES AND IMMUNOTOXINS THAT TARGET HUMAN GLYCOPROTEIN NMB - The invention provides high affinity antibodies suitable for forming Immunotoxins that inhibit the growth of cells expressing human glycoprotein NMB, including glioblastoma multiform cells, anaplastic astrocytoma cells, anaplastic oligodendroglioma cells, oligodendroglioma cells, and melanoma cells. | 2012-05-17 |
| 20120122122 | METHOD OF DIAGNOSING CANCER - A method of diagnosing cancer is provided. The method comprising determining a level of CEACAM1 on isolated peripheral blood lymphocytes (PBLs) of a subject in need thereof, wherein an upregulation of the level of CEACAM1 above a predetermined threshold is indicative of cancer in said subject. | 2012-05-17 |
| 20120122123 | DETECTION OF ANTHRAX PATHOGENICITY FACTORS - One major problem in diagnosis methods presently available for anthrax is that these methods require several days to produce a result. The only existing treatment for anthrax requires administration soon after infection at a time when patients are exhibiting only mild flu-like symptoms. Thus, a patient may be days beyond the time when treatment would be effective by the time a diagnosis is made. The present invention reduces diagnosis time to as little as four hours providing same day identification of anthrax radically increasing the odds of delivering proper treatment and patient recovery. The rapid identification of anthrax lethal factor activity exhibited by the instant invention is also amenable to in vivo screening protocols for the discovery and development of anthrax vaccines and lethal factor inhibitors. The instant invention isolates and concentrates lethal factor and lethal toxin from nearly any biological sample. By capitalizing on the endopeptidase activity of lethal factor the present invention amplifies output signals producing reliable detection of picomolar concentrations of lethal factor. The instant invention involves novel purification and detection techniques and substrates for rapid, reproducible, and quantitative measurements of anthrax lethal factor in biological samples. | 2012-05-17 |
| 20120122124 | NEW BACTERIOPHAGE ADHESION PROTEINS - The present invention relates to bacteriophage adhesion proteins binding to the O-antigen of gram negative bacteria, lacking the ability of binding to a bacteriophage and of hydrolysing lipopolysaccharides. The invention further relates to nucleic acid molecules comprising a sequence encoding the proteins according to the present invention. In addition, the present invention relates to a method for generating bacteriophage adhesion proteins according to the present invention. The invention further relates to the use of said proteins and methods of detection, purification and enrichment of bacteria. | 2012-05-17 |
| 20120122125 | ANTI-T. CRUZI ANTIBODIES AND METHODS OF USE - The present disclosure is directed to reagents and methods of using the reagents to detect epitopes of | 2012-05-17 |
| 20120122126 | ANTI-PSK ANTIBODY - An antibody which recognizes PSK is provided. | 2012-05-17 |
| 20120122127 | Cleavage Sensitive Antibodies and Methods of use thereof - We disclose cleavage-sensitive antibodies with epitopes spanning the scissile bond of the toxins molecular target protein, enabling toxin-associated proteolysis to be measured in a variety of assay formats. | 2012-05-17 |
| 20120122128 | IMMUNO-BASED BOTULINUM TOXIN SEROTYPE A ACTIVITY ASSAYS - The present specification discloses SNAP-25 compositions, methods of making α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P | 2012-05-17 |
| 20120122129 | RAPID NASAL ASSAY KIT - The present invention relates to an assay which can be used on nasal secretions. The assay is used to determine the cause of nasal secretions, for example whether the secretions are due to an allergic reaction or a non-allergic reaction. | 2012-05-17 |
| 20120122130 | Coenzyme-Linked Glucose Dehydrogenase and Polynucleotide Encoding the Same - The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor. | 2012-05-17 |
| 20120122131 | SIMULTANEOUS DETECTION OF METABOLIC ENZYME ACTIVITY AND METABOLITE LEVELS - Provided are methods for detecting a metabolic disorder in an individual using mass spectrometry. One method involves (a) contacting a sample containing (i) a metabolically indicative enzyme and (ii) a metabolic analyte, with a substrate for the enzyme to produce a reaction admixture, under conditions wherein the enzyme is capable of acting on a corresponding substrate to generate a product, and wherein a protease inhibitor is present; (b) contacting the reaction admixture with a reagent that inhibits the ability of the enzyme to act on a corresponding substrate, wherein the metabolic analyte and the product are soluble in the reagent; to produce a test sample and (c) determining the presence or amount of the metabolic analyte and the product contained in the test sample using mass spectrometry, wherein a determined presence or amount of the metabolic analyte and the product correlates with presence or absence of the metabolic disorder. | 2012-05-17 |
| 20120122132 | Protein Structural Biomarkers to Guide Targeted Chemotherapies - A rapid, infrared spectroscopic method has been developed to assess the efficacy of targeted chemotherapeutics against the structure of the polypeptide target, based on the effect of natural polymorphic sequence variation on the conformation of the protein. This method has an advantage over the current genomics-based screening, as the new method provides a direct readout of the structural, and hence functional, outcome of polymorphisms to the protein region targeted by drugs. It allows rapid measurement of a protein's susceptibility to therapeutic targeted agents, prior to using the drug as treatment in the patient. This method can be used to identify biomarkers for a response for a protein to a drug which can be readily tested, interpreted, and used in a clinical setting. | 2012-05-17 |
| 20120122133 | NOVEL DEVICES FOR THE DETECTION OF THE PRESENCE AND/OR ACTIVITY OF PROTEASES IN BIOLOGICAL SAMPLES - The subject invention provides novel devices and methods for the detection of the presence and/or activity of proteases in biological samples | 2012-05-17 |
| 20120122134 | Novel Peptidase Substrates - The present invention relates to the use of a compound of the following formula (I) for detecting a peptidase activity and/or a variation in pH: | 2012-05-17 |
| 20120122135 | Novel Peptidase Substrates - The present invention relates to the use of a compound of the following formula (I), as an enzyme substrate for the detection of a peptidase activity or as a pH indicator: | 2012-05-17 |
| 20120122136 | Self-immolative probes for enzyme activity detection - Provided is a compound comprising the structure: | 2012-05-17 |
| 20120122137 | Novel Nitroreductase Enzymatic Substrates - The invention relates to the use of a compound having formula (I) as an enzymatic substrate for the detection of a nitroreductase activity, wherein: W | 2012-05-17 |
| 20120122138 | CONSUMABLE COMPONENT KIT - According to the invention there is provided a consumable component kit for use in a system which utilises a liquid culture of a microbiological material, the kit including: a sealed and aseptic culturing vessel; at least one sealed and aseptic chamber for storing a liquid nutrient medium; a sample of the microbiological material; a sample of the nutrient medium which is suitable for use in a feedstock for the microbiological material; optionally, a sample of salts and other components of the liquid nutrient medium; and a plurality of aseptic conduits for connecting the chamber to the culturing vessel, and for connecting the culturing vessel to the remainder of the system to enable a liquid supply of the microbiological material, or a product or extract thereof, to be utilised by the system. | 2012-05-17 |
| 20120122139 | MICROFLUIDIC DEVICE AND HEMOGLOBIN MEASUREMENT METHOD USING THE SAME - A microfluidic device and a method for measurement of biomaterials using the same. The microfluidic device includes a microfluidic structure including: a sample chamber which receives and accommodates blood; a reagent chamber which contains a luminescent reactant; a first detection chamber which contains a first material that is positively charged; a second detection chamber which is connected to the first detection chamber, and contains a second material having a boronate moiety; and at least one channel which connects the sample chamber, the reagent chamber and the first and second detection chambers. | 2012-05-17 |
| 20120122140 | ULTRASONIC METHOD FOR MONITORING CELL CULTURES - A method for monitoring a cell culture, where the method includes measuring pulse-echo ultrasonic waveforms from the cell culture, and analyzing the pulse-echo ultrasonic waveforms to monitor the cell culture. | 2012-05-17 |
| 20120122141 | Point Source Diffusion Cell Activity Assay Apparatus - Apparatuses and methods for determining whether a test compound solution induces cell activity, an embodiment of the method of the present invention comprising placing a test compound solution in contact with a cell suspension media containing cells, diffusing the test compound solution into the cell suspension from a point source, and detecting activity in the cells with respect to their distance from the point source. Detecting activity in the cells can involve detecting activity in a first group of the cells proximate to the point source, and detecting activity in a second group of the cells farther from the point source than the first group. | 2012-05-17 |
| 20120122142 | CELL DEPOSITION SYSTEM - A system for depositing cells on an analysis plate, the cells being contained in a cell suspension including a fixing agent and the cells, included, for receiving the suspension, a chamber ( | 2012-05-17 |
| 20120122143 | TECHNIQUE FOR DETERMINING MATURITY OF A CELL AGGREGATION, IMAGE PROCESSING PROGRAM AND IMAGE PROCESSING DEVICE USING THE TECHNIQUE, AND METHOD FOR PRODUCING A CELL AGGREGATION - An image processing program (GP) is configured to comprise a step (A | 2012-05-17 |
| 20120122144 | METHODS EMPLOYING INSECT MODELS FOR DETERMINING INTESTINAL ABSORPTION OF CHEMICAL COMPOUNDS - There is provided insect screening models to determine gastrointestinal absorption of different chemical compounds in vertebrates, and in particular humans, in order to improve the compound screening procedures/processes in the early drug discovery process. This offers many advantages relative to prior technologies since insect models are more reliable tools for the decision-making process than the existing in vitro models, and will speed up the drug screening process and reduce the late phase attrition rate. Moreover, it will reduce the number of mammals sacrificed during the drug discovery phase. | 2012-05-17 |
| 20120122145 | INSECT-BASED EX VIVO MODEL FOR TESTING BLOOD-BRAIN BARRIER PENETRATION AND METHOD FOR EXPOSING INSECT BRAIN TO CHEMICAL COMPOUNDS - There is provided an ex-vivo insect screening model to accurately determine blood-brain barrier penetration of different chemical compounds in order to improve the compound screening procedures/processes in the early drug discovery process. This object offers many advantages relative to prior technologies since insect models are more reliable tools for the decision-making process than the existing in vitro models, and will speed up the drug screening process and reduce the late phase attrition rate. Moreover, it will reduce the number of mammals sacrificed during the drug discovery phase. | 2012-05-17 |
| 20120122146 | NANOPARTICLE SCREENING METHODS EMPLOYING INSECTS WITH BLOOD BRAIN BARRIER - There is provided an insect model for determining the penetration of nanoparticles through the BBB. The model is also directed to the use of insects for studying the environmental safety of nanoparticles. The particles are administered into the hemolymf of an insect having a BBB, such as the locust, and the uptake into the brain is analysed. Thus, in this model it is possible to investigate the potential up-take into the brain of nanoparticles circulating in the blood. | 2012-05-17 |
| 20120122147 | REAL-TIME METHOD FOR THE DETECTION OF VIABLE MICRO-ORGANISMS - The invention relates to a method for real-time detection of viable microorganisms comprising: a. addition of a cell-permeable, phototautomeric compound to a micro-organism or other living cell; and b. measuring the fluorescent emission of said phototautomeric compound. Preferably the phototautomeric compound is salicylic acid, 2-hydroxy-1-naphtoic acid or 1-hydroxy-2-naphtoic acid. Further, the assay can he used to assess the antibiotic effect of a test compound. This test can be used as a high—throughput screening for compounds with antibiotic activity. Also part of the invention is the use of a cell permeable phototautomeric compound in a method for determining the viability of micro-organisms and for assessing the antibiotic effect of a test compound. | 2012-05-17 |
| 20120122148 | Media For The Specific Detection Of Gram-Negative Bacteria Resistant To Beta-Lactam Antibiotics - The invention relates to a reaction medium for gram-negative bacteria having a beta-lactam antibiotic resistance mechanism, comprising:
| 2012-05-17 |
| 20120122149 | Absorbent bead concentration method that eliminates the need for centrifugation - This invention is a technique that enables the concentration of cells, microorganisms, parasite eggs and cysts using absorbent beads, eliminating the need for centrifugation. | 2012-05-17 |
| 20120122150 | STABILIZATION METHOD FOR BIOLOGICAL SAMPLES BY COMBINATION OF HEATING AND CHEMICAL FIXATION - The present invention provides methods for stabilizing a biological sample for analysis. The invention more particularly provides methods combining heat treatment and chemical fixation of biological samples in order to maintain protein primary structure and post-translational modifications, such as protein phosphorylations. | 2012-05-17 |
| 20120122151 | CYTOLOGICAL OR HISTOLOGICAL BINDING COMPOSITION AND STAINING METHODS - A histological and cytological fixing composition, includes at least alcohol, ethylene glycol, dimethyl sulfoxide, water, and sodium chloride. Also, process for preparing this fixer, as well as to its use in particular in processes for staining cells or cellular structures are described. | 2012-05-17 |
| 20120122152 | TREATING BIOMASS TO PRODUCE MATERIALS USEFUL FOR BIOFUELS - Fermentable sugar useful for the production of biofuels can be produced from biomass by contacting the biomass with a solution containing at least one α-hydroxysulfonic acid. The α-hydroxysulfonic acid can be easily removed from the product and recycled. | 2012-05-17 |
| 20120122153 | ACID-CLEVABLE LINKERS EXHIBITING ALTERED RATES OF ACID HYDROLYSIS - An acid-cleavable peptide linker comprising aspartic acid and proline residues is disclosed. The acid-cleavable peptide linker provides an altered sensitivity to acid-hydrolytic release of peptides of interest from fusion peptides of the formula PEP1-L-PEP2. The inventive linker, L, is described in various embodiments, each of which provides substantially more rapid acid-release of peptides of interest than does a single aspartic acid-proline pair. In an additional aspect, a method of increasing the stability of an acid cleavable linkage to acid hydrolysis is also provided. | 2012-05-17 |
| 20120122154 | METHOD OF PRODUCING LIPIDATED POLYPEPTIDES - A method of producing a recombinant lipidated polypeptide in | 2012-05-17 |
| 20120122155 | IMMORTALIZED AVIAN CELL LINES AND USE THEREOF - The present invention relates to specific immortalized avian cell lines expressing telomerase reverse transcriptase (TERT), and exhibiting distinct biologics production patterns. More particularly, the present invention relates to immortalized avian cell line capable of either amplifying Flaviviridae but not capable of amplifying Vaccinia virus strain Copenhagen (W—COP) nor Modified Vaccinia virus Ankara (MVA), or capable of amplifying both Flaviviridae and Poxviridae. The invention further relates to the use of said immortalized avian cell lines and related methods for producing biologics, including viruses and proteins. | 2012-05-17 |
| 20120122156 | RECOMBINANT PROTEIN OF FIBROBLAST GROWTH FACTOR HAVING ADHESIVE ACTIVITY FOR STEM CELLS AND METHOD FOR CULTURING STEM CELLS USING THE SAME - The present invention relates to a recombinant protein of a fibroblast growth factor (FGF) having an adhesive activity for stem cells and a method for culturing stem cells using the same. More particularly, the present invention relates to a recombinant protein having an adhesive activity for stem cells by fusion of a polypeptide linker at amino terminal of FGF, and a method for culturing stem cells using immobilized FGF comprising: fixing the recombinant protein in a culture vessel with a hydrophobic surface using amino terminal of the polypeptide linker, adhering stem cells on the recombinant protein-fixed culture vessel, and culturing the stem cells. | 2012-05-17 |
| 20120122157 | Signal Peptide for Producing a Polypeptide - The present invention relates to a method for producing a polypeptide comprising using a signal peptide, to nucleic acid constructs comprising a first nucleotide sequence encoding the signal peptide and a second nucleotide sequence encoding a polypeptide which is foreign to the first nucleotide sequence. Furthermore, it also relates to expression vectors and host cells comprising the nuclei acid construct. | 2012-05-17 |
| 20120122158 | PCR FOR DNA THAT IS RESISTANT TO AMPLIFICATION - DNA that is difficult to amplify by conventional PCR is amplified by a modified PCR process that includes a high-temperature, short-term heating step as the last step of each thermal cycle, following the conventional denaturing and annealing/elongation steps. | 2012-05-17 |
| 20120122159 | PCR-BASED METHOD OF SYNTHESIZING A NUCLEIC ACID MOLECULE - There is provided a method of synthesizing a nucleic acid molecule and in one aspect, the method comprises assembling a full length template nucleic acid molecule by PCR in a PCR reaction mixture comprising a set of assembly oligonucleotides having a first average melting temperature and a set of outer amplification primers having a second average melting temperature that is lower than the first average melting temperature, wherein said assembling comprises subjecting the PCR reaction mixture to a first annealing temperature that is higher than the second average melting temperature and; amplifying the full length template nucleic acid molecule by PCR in the PCR reaction mixture wherein said amplifying comprises subjecting the PCR reaction mixture to a second annealing temperature that permits annealing of the outer amplification primers to the full length template nucleic acid molecule. | 2012-05-17 |
| 20120122160 | THERMAL CYCLER AND THERMAL CYCLING METHOD - A thermal cycler includes a holder to which a biotip having a longitudinal direction is attached in such a manner that one end portion of the biotip is at a higher level than the other end portion, and that the distance between one end portion of the biotip and the rotational axis is shorter than the distance between the other end portion of the biotip and the rotational axis, a heating unit heats a first end portion of the biotip, a rotating unit rotates the holder, and a controller that controls the rotation speed of the rotating unit. The controller has a first mode a rotation speed at which the magnitude of the centrifugal force acting on the reaction mixture becomes smaller than the gravity, and a second mode a rotation speed at which the magnitude of the centrifugal force acting on the reaction mixture becomes greater than the gravity. | 2012-05-17 |
| 20120122161 | Sorting Asymmetrically Tagged Nucleic Acids by Selective Primer Extension - The present invention provides methods and compositions for amplifying and sorting adapter tagged nucleic acid fragments using selective primer extension. Immortalized pooled polynucleotide samples and method of producing the same are also provided. | 2012-05-17 |
| 20120122162 | ENZYMATIC HYDROLYSIS OF PRE-TREATED BIOMASS - This disclosure relates to a method for performing high solids saccharification comprising by (a) providing a cellulosic biomass; (b) pretreating the cellulosic biomass in a pretreatment process to produce a pretreated cellulosic biomass; (c) adjusting said pretreated cellulosic biomass to a solids concentration of 6% to 35% w/w and a starting pH of between 5-7; and (d) hydrolyzing the pretreated biomass with at least one aqueous hydrolyzing liquid comprising at least one enzyme selected from the group consisting of a cellulase, a saccharification enzyme, and a combination thereof for a period of time, to hydrolyze at least a part of the pretreated cellulosic biomass to a cellulosic hydrolysate, said cellulosic hydrolysate comprising fermentable sugars. | 2012-05-17 |
| 20120122163 | Microorganism Which Produces L-Amino Acid and Method for Producing L-Amino Acid Using the Same - The present invention relates to a microorganism belonging to the genus | 2012-05-17 |
| 20120122164 | METHOD AND SYSTEM FOR PROCESSING A BIOMASS FOR PRODUCING BIOFUELS AND OTHER PRODUCTS - The present invention provides methods of processing a biomass, e.g., for producing lipid and non-lipid materials, by inducing autolysis of a biomass. The biomass may be generated using any species of microorganism and any available biodegradable substrate, including waste materials. The lipid and non-lipid materials are present in two separate layers of the autolysate, and they can be used to generate valuable products such as biofuels and nutritional supplements. The present invention further provides a processing apparatus useful for practicing the methods of the present invention. | 2012-05-17 |
| 20120122165 | RECOMBINANT MICROORGANISM AND METHOD FOR PRODUCING ALIPHATIC POLYESTER WITH THE USE OF THE SAME - This invention provides a recombinant microorganism exhibiting excellent aliphatic polyester productivity and a method for producing an aliphatic polyester with the use of the recombinant microorganism. In this invention, the | 2012-05-17 |
| 20120122166 | MUTANT METHYLGLYOXAL SYNTHASE (MGS) FOR THE PRODUCTION OF A BIOCHEMICAL BY FERMENTATION - The present invention concerns a method for the production of a biochemical selected among lactic acid, acetol and 1,2-propanediol, comprising culturing a microorganism modified for an improved production of the biochemical selected among lactic acid, acetol and 1,2-propanediol in an appropriate culture medium and recovery of the desired biochemical which may be further purified wherein the microorganism expresses a methylglyoxal synthase (MGS) enzyme which activity is not inhibited by orthophosphate. | 2012-05-17 |
| 20120122167 | METHOD FOR PRODUCING LACTIC ACID - Provided is a method for producing lactic acid, which includes: obtaining D-lactic acid or L-lactic acid by carrying out lactic acid fermentation using a lactic acid-producing microorganism under a pressurized condition that exceeds normal pressure and is capable of maintaining lactic acid production activity of the lactic acid-producing microorganism. | 2012-05-17 |
| 20120122168 | RECOMBINANT ESCHERICHIA COLI HAVING ENHANCED ACETYL-COENZYME A SYNTHETASE ACTIVITY FOR PRODUCING GLYEROL AND GLYCEROL-DERIVED PRODUCTS - Recombinant | 2012-05-17 |
| 20120122169 | MUTANT YQHD ENZYME FOR THE PRODUCTION OF A BIOCHEMICAL BY FERMENTATION - The present invention concerns a method for the production of a biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol comprising culturing a microorganism modified for an improved production of the biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol in an appropriate culture medium and recovery of the desired biochemical which may be further purified wherein the microorganism expresses a YqhD enzyme which catalytic efficiency toward NADPH is increased. | 2012-05-17 |
| 20120122170 | IN SITU PRODUCTION OF FURFURAL IN A CONTROLLED AMOUNT IN AN ALCOHOL PRODUCTION UNIT FROM A LIGNOCELLULOSIC BIOMASS - The present invention describes a process for the production of alcohol, known as second generation production, from a lignocellulosic substrate, in which a controlled quantity of furfural is produced in situ and which comprises at least the following steps:
| 2012-05-17 |
| 20120122171 | COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS - The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided is a method are methods for the production of 4-HB and BDO. | 2012-05-17 |
| 20120122172 | METHOD FOR PRODUCING ETHANOL FROM XYLOSE USING RECOMBINANT SACCHAROMYCES CEREVISIAE TRANSFORMED TO ELIMINATE FUNCTIONS OF GENES INVOLVED IN TOR SIGNAL TRANSDUCTION PATHWAY - Disclosed is a method for producing ethanol from zylose using a | 2012-05-17 |
| 20120122173 | Methods for Increasing the Production of Ethanol from Microbial Fermentation - A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing in a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity of greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1. | 2012-05-17 |
| 20120122174 | Non-sterile fermentation of bioethanol - A range of concentrations exists in which fermentation inhibitors derived from pretreatment of lignocellulosic feed stocks inhibit growth of lactic acid bacteria without affecting fermentive yeast. By optimizing levels of fermentation inhibitors to fall within this range, yeast fermentations of lignocellulosic biomass can be conducted under non-sterile conditions with ethanol yields comparable to those achieved under sterile conditions. Optimised inhibitor levels can be achieved by controlling the water/biomass ratio of a lignocellulosic biomass during and after pretreatment, for example by washing the fiber fraction of a previously pretreated lignocellulosic biomass with a pre-defined amount of fresh water or recycled process solutions. Crude extracts of liquid fraction or process solutions from pretreatment of lignocellulosic biomass can also provide an effective anti-baterial treatment for first generation starch fermentations. | 2012-05-17 |
| 20120122175 | METHOD AND DEVICE FOR OPERATING A FERMENTATION PLANT - A method for operating a fermentation plant with at least one digester ( | 2012-05-17 |
| 20120122176 | CELLULASE CEL5H RELATED REAGENTS AND THEIR USE IN MICROORGANISMS - The invention relates to applications of the cellulase Cel5H of | 2012-05-17 |
| 20120122177 | Viral Infection Enhancing Peptide - The invention relates to a peptide derived from HIV-1 gp120 which forms insoluble aggregates when introduced into an aqueous solution and its use for enhancing viral infection of cells. In addition, the invention comprises methods for enhancing viral infection of cells, for concentrating a virus and for separating a virus from a fluid. | 2012-05-17 |
| 20120122178 | Human Aminoacyl-tRNA Synthetase Polypeptides Useful for the Regulation of Angiogenesis - The present invention provides an isolated polypeptide comprising residues 94-471 of SEQ ID NO: 10, wherein the isolated polypeptide does not comprise amino acid residues 1-47 of SEQ ID NO: 10. Nucleic acids and expression vectors encoding the peptide also are provided. | 2012-05-17 |
| 20120122179 | MEANS FOR PURIFYING A PROTEIN OF BLOOD PLASMA AND METHODS FOR IMPLEMENTING SAME - An affinity substrate for the selective binding of a protein of blood plasma includes a solid substrate material on which are immobilized deoxyribonucleic aptamers specifically binding with the plasma protein. | 2012-05-17 |
| 20120122180 | INCORPORATION OF TYPE III POLYKETIDE SYNTHASES INTO MULTIDOMAIN PROTEINS OF THE TYPE I AND III POLYKETIDE SYNTHASE AND FATTY ACID SYNTHASE FAMILIES - Recombinant fusion proteins in which intermediates are covalently bound to the fusion proteins and transferred between domains of the fusion proteins are provided. The fusion proteins include proteins having type I polyketide or fatty acid synthase domains fused with type III polyketide synthase domains. Methods of making such recombinant fusion proteins and methods using such proteins to produce polyketide and other products are described. | 2012-05-17 |
| 20120122181 | METHOD FOR DETERMINING ANTAGONIST ACTIVITY TO A CYTOKININ RECEPTOR - The present invention provides a method for analyzing agonist-activity to a cytokinin receptor, which comprises (1) bringing an examinee substance into contact with a transformed cell into which DNA coding the cytokinin receptor is introduced and (2) measuring the existence or the quantity of intracellular signal transduction from the cytokinin receptor expressed in the transformed cell, and, a method for analyzing antagonist-activity to a cytokinin receptor, which comprises (1) bringing an examinee substance and a substance having agonist-activity to the cytokinin receptor into contact with a transformed cell into which DNA coding the cytokinin receptor is introduced. | 2012-05-17 |
| 20120122182 | GAUSSIA LUCIFERASE VARIANT FOR HIGH-THROUGHPUT SCREENING - Described herein is a variant of wild type Gaussia luciferase that catalyzes glow-type emission kinetics suited for high-throughput functional screening applications. Polypeptides, functional fragments, variants, and nucleic acids that encode the enhanced luciferase are further described. One such polypeptide corresponds to wild type Gaussia luciferase with a substitution mutation of I for M at position 43 of the mature peptide. Methods of use, assay systems and kits that contain the polypeptides and/or nucleic acids are further described. | 2012-05-17 |
| 20120122183 | FLOCCULATION WITH DIVALENT SALT AND PHOSPHATE - A method of flocculating a bacterial cell, producing a protein of interest, from a fermentation broth, comprising a) diluting the fermentation broth up to 1000% (w/w) with water; b) adding a divalent salt to a concentration in the fermentation broth of more than 10 milli moles per liter diluted fermentation broth; c) adjusting the phosphate concentration to a concentration in the fermentation broth of more than 10 milli moles per liter diluted fermentation broth; d) adjusting the pH of the diluted fermentation broth to a pH within the range of 6.1-10.5; and e) removing the bacterial cells, whereby a protein solution with a turbidity of less than 100 NTU is obtained. | 2012-05-17 |
| 20120122184 | Polypeptides of strain Bacillus sp. P203 - A new strain | 2012-05-17 |
| 20120122185 | Chimeric viruses presenting non-native surface proteins and uses thereof - The present invention provides chimeric negative-stand RNA viruses that allow a subject, e.g., an avian, to be immunized against two infectious agents by using a single chimeric virus of the invention. In particular, the present invention provides chimeric influenza viruses engineered to express and incorporate into their virions a fusion protein comprising an ectodomain of a protein of an infectious agent and the transmembrane and cytoplasmic domain of an influenza virus protein. Such chimeric viruses induce an immune response against influenza virus and the infectious agent. The present invention also provides chimeric Newcastle Disease viruses (NDV) engineered to express and incorporate into their virions a fusion protein comprising the ectodomain of a protein of an infectious agent and the transmembrane and cytoplasmic domain of an NDV protein. Such chimeric viruses induce an immune response against NDV and the infectious agent. | 2012-05-17 |
| 20120122186 | NOVEL BACTERIOPHAGE STRAINS FOR THE TREATMENT OF BACTERIAL INFECTIONS, ESPECIALLY DRUG RESISTANT STRAINS OF THE GENUS ENTEROCOCCUS - Novel bacteriophage strains are disclosed and their use in the production of preparations for use in the treatment of bacterial infections, particularly with drug resistant strains of bacteria of the genus | 2012-05-17 |
| 20120122187 | METHOD FOR INCREASING THE CONCENTRATION OF COLONIES OF MICRO ORGANISMS IN A PROCESS FOR REMOVING CONTAMINANTS BY ANAEROBIC DIGESTION - The present invention refers to a process to increase the concentration of micro organisms' colonies which are formed on the surface of | 2012-05-17 |
| 20120122188 | LIVE ORGANISM PRODUCT - A live organism product wherein the organisms are in a dormant state and are suspended in a liquid carrier which is sufficiently devoid of moisture so that the organisms will remain in a dormant state for several months. The carrier is comprised of oil. The carrier may also include an absorbent. The product is stored and shipped in a plastic bag and is sprayed onto its target host or the like. The moisture and pH then activates the organisms. | 2012-05-17 |
| 20120122189 | Lactobacillus Mutant - The present invention discloses a | 2012-05-17 |
| 20120122190 | Red Mold Mutant that can Increase the Production of Monascus Metabolites - The present invention discloses a Red mold mutant that can increase the production of monascus metabolites, wherein the Red mold mutant is | 2012-05-17 |
| 20120122191 | Methods For Producing Secreted Polypeptides - The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3′ end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides. | 2012-05-17 |
| 20120122192 | Edible Oils and Food Compositions From Heterotrophic Microorganisms - The invention provides methods of manufacturing oils and oil-based products such as transportation fuels, industrial chemicals, edible oils, lubricants and plastics using sugar cane, sugar cane juice, corn steep liquor, corn starch or depolymerized cellulosic material as a feedstock for bioproduction processes. The disclosed processes utilize oil-bearing microbes as a food source and as a conversion technology to convert chemical energy produced by various fixed carbon sources into edible oils. | 2012-05-17 |
| 20120122193 | ENGINEERED CO2 FIXING MICROORGANISMS PRODUCING CARBON-BASED PRODUCTS OF INTEREST - The present disclosure identifies pathways and mechanisms to confer production of carbon-based products of interest such as ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof in photoautotrophic organisms such that these organisms efficiently convert carbon dioxide and light into carbon-based products of interest, and in particular the use of such organisms for the commercial production of ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof. | 2012-05-17 |
| 20120122194 | METHOD FOR THE TOTAL GASIFICATION OF GARBAGE OR WASTE - A method for treating garbage or waste in which the garbage or refuse is subjected to at least one compression step ( | 2012-05-17 |
| 20120122195 | PROCESS FOR CO2 CAPTURE USING MICRO-PARTICLES COMPRISING BIOCATALYSTS - A process for capturing CO | 2012-05-17 |
| 20120122196 | Methods and Products for Biomass Digestion - Provided herein are methods and products for biomass digestion, which includes the production of biogas, U.S. Environmental Protection Agency classified Class A Biosolids, and pathogen reduced organic liquid fertilizer. Through the digestion of waste materials using sequential phases in an efficient digestion process, enhanced biomass conversion efficiency and improved output of products (in quantity and/or quality) are obtained with a significant reduction in dwell time in each phase. | 2012-05-17 |
| 20120122197 | INKJET REAGENT DEPOSITION FOR BIOSENSOR MANUFACTURING - A technique for producing a biosensor includes inkjet printing a reagent onto electrodes of the biosensor. The ink has been specially formulated to allow the reagent to be printed using inkjet printing while at the same time produce commercially viable biosensor. The inkjet printing of the reagent allows for different inkjet patterns to be produced as well as facilitates quick change over between various products. For example, the technique allows the reagent and electrode to be formed on opposite sides of a substrate. In another example, the reagent can be layered such that incompatible reagents can be separated by a barrier layer. The electrodes for the biosensor can also be inkjet printed such that most of the biosensor can be produced using inkjet technology. | 2012-05-17 |
| 20120122198 | BIOSENSOR MANUFACTURING METHOD - A biosensor having a first conductive component is described, wherein the first conductive component includes at least one boundary formed by a first processing technique and at least one boundary formed by a second processing technique not the same as the first processing technique. The biosensor can also have a second conductive component including at least one boundary formed by the first processing technique and at least one boundary formed by a third processing technique not the same as the first processing technique. Further, the biosensor has a third conductive component including at least one boundary formed by the second processing technique and at least one boundary formed by the third processing technique not the same as the second processing technique. | 2012-05-17 |
| 20120122199 | Photobioreactor for the growth and development of photosynthetic and heterotrophic microorganisms - A photobioreactor, especially for the growth and development of photosynthetic microorganism such as microalgae or cyanobacteria. The photobioreactor includes at least one reflector ( | 2012-05-17 |
