18th week of 2010 patent applcation highlights part 53 |
Patent application number | Title | Published |
20100112619 | LIGAND-CONTAINING MICELLES AND USES THEREOF - Ligand-containing micelles and various compositions, kits and methods for their preparation and use are provided. | 2010-05-06 |
20100112620 | BIORHYTHM INFORMATION ACQUISITION METHOD - A method of acquiring information on a biorhythm on the basis of a variation with time of the quantity of a physiologically active substance in tear and/or saliva is provided. | 2010-05-06 |
20100112621 | Analytical Method for Protein Mapping Using Hydrogen/Deuterium Exchange - Analytical methods using hydrogen/deuterium exchange are provided which reduce or eliminate the back-exchange of deuterium for hydrogen. The methods, which are useful in protein and peptide mapping, include the steps of (a) providing a peptide or protein comprising a solvent accessible hydrogen; (b) exchanging the solvent accessible hydrogen for a deuterium; (c) separating the peptide or protein with supercritical fluid chromatography; and (d) analyzing by mass spectrometry the mass of the separated peptide or protein. Supercritical fluid chromatography enables the observation of fast exchanging hydrogen atoms missed using conventional liquid chromatography methods. | 2010-05-06 |
20100112622 | METHOD FOR DETECTING PHENOTHIAZINE-DERIVATIVE COLOR AND COLOR-DEVELOPER REAGENT USED THEREIN - The present invention provides a phenothiazine-derivative color-measuring method that can detect a phenothiazine-derivative color even at a wavelength longer than the wavelength that exhibits maximum absorption. A phenothiazine-derivative color is detected, by adding a 5-hydroxy-1-(4-sulfophenyl)-4-(4-sulfophenylazo)pyrazole-3-carboxylic acid salt, 6-hydroxy-5-(4-sulfophenylazo)-2-naphthalenesulfonic acid salt, 3-hydroxy-4-(4-sulfonaphthylazo)-2,7-naphthalenedisulfonic acid salt, 7-hydroxy-8-(4-sulfonaphthylazo)-1,3-naphthalenedisulfonic acid salt, 3′,6′-dihydroxy-2′,4′,5′,7′-tetraiodospiro[isobenzofuran-1(3H),9′-(9H)xanthene]-3-one salt, 3′,6′-dihydroxy-2′,4′,5′,7′-tetrabromo-4,5,6,7,-tetrachlorospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-3-one salt, 4,5,6,7-tetrachloro-3′,6′-dihydroxy-2′,4′,5′,7′-tetraiodospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-3-one salt or flavonoid-based color to the reaction system containing a phenothiazine-derivative color, and then measuring the light absorbance at a wavelength of 610 to 730 nm. | 2010-05-06 |
20100112623 | Bacteria analyzer, method for analyzing bacteria, and a computer program product - A bacteria analyzer comprising: a sample preparing section for preparing a measurement sample from a specimen; a detector for detecting bacteria contained in the measurement sample prepared by the sample preparing section; and a controller including a memory under control of a processor, the memory storing instructions enabling the processor to carry out operations, comprising: obtaining the distribution state of bacteria detected by the detector; setting a bacteria count region according to the obtained bacteria distribution state; and counting the number of bacteria contained in the set bacteria count region is disclosed. Method for analyzing bacteria, and a computer program product for bacteria analyzer are also disclosed. | 2010-05-06 |
20100112624 | METHOD FOR OPERATING A TISSUE PROCESSOR, AND TISSUE PROCESSOR - A method for operating a tissue processor and a respective tissue processor for performing this method are described for the processing tissue samples. The tissue processor comprises at least one retort for receiving the tissue samples and at least one container for receiving a process medium. The process medium is transferred at least one of from the container into the retort and from the retort into the container. A value is automatically measured in the course of transferring the process medium, the value representing a characteristic property of the process medium. The process medium is identified based on the value. | 2010-05-06 |
20100112625 | METHOD AND APPARATUS FOR PROCESSING TISSUE SAMPLES USING A SENSOR - An apparatus and a method for processing tissue samples are described. The apparatus comprises: at least one retort for accommodating tissue samples; at least one container for storing alcohol or xylene; a valve adapted to connect the at least one retort with the at least one container depending on an operating position of the valve; and at least a first sensor that is arranged in flow direction between the container and the retort for measuring a value of a parameter that represents a purity level of the alcohol or xylene; wherein the first sensor and the valve are configured to replace the alcohol or xylene depending on the value of the parameter that represents the purity level. The method comprises for the alcohol or xylene conducting between the container and the retort; automatically measuring the purity level and replacing depending on the purity level. | 2010-05-06 |
20100112626 | Use of Semenogelin in the Diagnosis, Prognosis and Treatment of Cancer - Method of diagnosing cancer in a mammal comprising assaying a test sample from the mammal for an increased level of semenogelin, wherein the increased level of semenogelin in the test sample is diagnostic for the cancer; methods of prognosticating and assessing the effectiveness of treatment of a cancer in a mammal based on the level of semenogelin in a test sample; a composition comprising (a) an immune-response inducing effective amount of (i) semenogelin or polypeptide fragment thereof or (ii) antibody thereto or (b) a recombinant vector encoding and expressing an immune-response inducing effective amount of (i) or (ii); a method of inducing an immune response to a cancer in a mammal comprising administering to the mammal the foregoing composition. | 2010-05-06 |
20100112627 | System and Method for Displaying Three-Dimensional Object Scattergrams - System and method for displaying three-dimensional object scattergrams of particles are provided. In one embodiment, at least two parameters associated with at least one particle in a biological sample are detected and stored as data. An initial two-dimensional scattergram of the data is created with the two dimensions corresponding to the two parameters, and each data point corresponding to a particle in the biological sample. A data point in the initial two-dimensional scattergram is categorized into a population corresponding to a particle population. A density value of the data point is evaluated. Color data for the data point is calculated based on the evaluated density value and the categorized population. A three-dimensional location is generated based on the location in the initial two-dimensional scattergram and a property of the data point. A geometric shape centered at the generated three-dimensional location is displayed using the calculated color data. | 2010-05-06 |
20100112628 | METHODS AND ASSAYS FOR DETECTING AND QUANTIFYING PURE SUBPOPULATIONS OF WHITE BLOOD CELLS IN IMMUNE SYSTEM DISORDERS - Methods for detecting nonactivated basophils in a whole blood sample obtained from a normal healthy subject, methods for determining a subject's susceptibility to an allergic reaction to an allergen, where the subject has no known allergy to the allergen, methods for measuring a response to challenge with a potential allergen in a whole blood sample obtained from a subject with known allergic reactivity to allergens other than the potential allergen; and an in vitro system for reliable detection or quantification of a specific white blood cell population in a whole blood sample are described. | 2010-05-06 |
20100112629 | CELL BLOCK CASSETTE DEVICE - A tissue cassette assembly includes a housing having a recess formed therein, and a compressible reservoir disposed partially or wholly inside of, or otherwise attached in fluid communication with, the housing recess, the compressible reservoir containing a tissue embedding material. The tissue cassette further includes a port disposed in the housing, the port in fluid communication with the compressible reservoir at one end and terminating in a sample cavity at another end. During operation, the compressible reservoir is compressed or squeezed to release the tissue embedding material into the sample cavity containing the biological sample. | 2010-05-06 |
20100112630 | METHODS FOR MEASURING MICROBIOLOGICAL CONTENT IN AQUEOUS MEDIA - A process for measuring total microbiological content in an aqueous medium includes adding a fluorescent dye to the aqueous medium, measuring the fluorescent signal in the aqueous medium to obtain a baseline fluorescent signal, releasing intracellular content of the microbiological matter into the aqueous medium by lysing the microbiological matter, measuring the fluorescent signal in the aqueous medium with the released intracellular content of the microbiological matter to obtain a second fluorescent signal, subtracting the baseline signal from the second fluorescent signal to obtain a net fluorescent signal and equating the net fluorescent signal with a microbiological content. Methods for measuring biofilm and adjusting for background noise are also provided. | 2010-05-06 |
20100112631 | Chip Having Microchannel For Counting Specific Micro Particles Among Floating Micro Particle Mixture By Optical Means And A Method For Counting Micro Particles Using The Same - A microchannel chip for counting floating microparticles with an optical method is provided. The depth of the microchannel chip is made to be a depth of field such that a clear and sharp optical image of microparticles can be obtained, without necessarily diluting the microparticle mixture inside the microchannel. Consequently, a microparticle counting instrument can easily count microparticles shown on an optical image of the microchannel. Particularly, it enables to count more easily the number of CD3 cells CD4 cells or CD8 cells in white blood cells being stained, without lysing or diluting red blood cells. Therefore, the microchannel chip can advantageously used for counting the number of CD3 cells, CD4 cells or CD8 cells in blood of an AIDS patient to monitor the progression of AIDS. | 2010-05-06 |
20100112632 | COMPOSTER MECHANISM - A rotary composter having a fuselage member where a drive system is configured to rotate the fuselage member where the end caps are substantially stationary and do not rotate and the fuselage member has a plurality of longitudinally extending slats contained therein. | 2010-05-06 |
20100112633 | Two-Step Enzyme Method for Preparing 7-Aminocephalosporanic Acid - The present invention discloses a two-step enzyme method for preparing 7-aminocephalosporanic acid from cephalosporin C, wherein D-amino acid oxidase used is purified D-amino acid oxidase mutant of yeast | 2010-05-06 |
20100112634 | YEAST STRAINS AUTONOMOUSLY PRODUCING STEROIDS - The present invention relates to genetically modified yeast strains autonomously producing, from a simple carbon source, steroids. The invention also relates to a method for producing steroids from such yeast strains. | 2010-05-06 |
20100112635 | Protein hydrolysates enriched in peptides having a carboxy terminal proline residue - A method of enzymatically producing a protein hydrolysate from a protein substrate is described, wherein a proline-specific endoprotease or a composition containing a proline-specific endoprotease and optionally a subtilisin or a metallo endoprotease, and other enzymes such as carboxypeptidases, is used to produce a protein hydrolysate enriched in peptide fragments having a carboxy-terminal proline residue. Such protein hydrolysates may be used as such or to reduce bitterness in foods nutritionally supplemented by protein hydrolysates, as well as to produce hydrolysate-containing foodstuffs having low antigenicity. | 2010-05-06 |
20100112636 | INDUCIBLE/REGULATED GENE EXPRESSION SYSTEM IN E COLI - An expression system for transforming | 2010-05-06 |
20100112637 | Polypeptides Having Alpha-Amylase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having alpha-amylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. | 2010-05-06 |
20100112638 | RECOMBINANT HOST CELL FOR THE PRODUCTION OF A COMPOUND OF INTEREST - We describe a recombinant host cell for the production of a compound of interest as well as isolated fungal promoter DNA sequences, to DNA constructs, vectors, and fungal host cells comprising these promoters in operative association with coding sequences encoding a compound of interest. We also describe methods for expressing a gene of interest and/or producing compounds of interest using a promoter according to the invention. | 2010-05-06 |
20100112639 | ENHANCED PRODUCTION AND PURIFICATION OF A NATURAL HIGH INTENSITY SWEETENER - Recombinant | 2010-05-06 |
20100112640 | POLYNUCLEOTIDE - The present invention relates to a polynucleotide comprising a ubiquitous chromatin opening element (UCOE) which is not derived from 13. The polynucleotide of any one of claims | 2010-05-06 |
20100112641 | PROCESS FOR PRODUCING AND PURIFYING FACTOR VIII AND ITS DERIVATIVES - Disclosed is a method for producing proteins having factor VIII procoagulant activity in serum-free medium by in vitro culturing of mammalian cells, wherein the serum-free medium contains an inhibitor against the protease released from cultured cells. In accordance with this invention, the inhibitor can protect the cleavage of a target protein during cultivation and increase homogeneity of a target molecule, wherein the inhibitor can be a dextran sulfate. This invention also relates to a method of purifying target molecules from the culture medium containing both a target molecule and selected inhibitors by affinity chromatography. | 2010-05-06 |
20100112642 | Production Of CPD Glycosylases - Recombinant forms of DNA sequences for CPD glycosylases, including the bacteriophage T4 gene denV, are described that are capable of expression at high levels. Active CPD glycosylases can be recovered from inclusion bodies resulting from the high expression using, for example, a homogenization process which employs stream mixing, and the active proteins can be used in, for example, topical formulations for treatment of photosensitive diseases. Stream mixing can also be used to solubilize inclusion bodies containing proteins other than CPD glycosylases. | 2010-05-06 |
20100112643 | METHOD FOR DIRECT CAPTURE OF RIBONUCLEIC ACID - A method of: providing a solid surface having a dendrimer molecule bound thereto and a single-stranded probe nucleic acid immobilized to the dendrimer; contacting the solid surface with a sample suspected or known to contain a target ribonucleic acid; denaturing the target ribonucleic acid; and incubating the sample to allow hybridization of the denatured ribonucleic acid to the probe nucleic acids. The target ribonucleic acid is complementary to the probe nucleic acid. | 2010-05-06 |
20100112644 | Methods for detection and quantitation of small RNAs - Improved methods that increase the specificity and sensitivity of detection of small RNAs, including miRNAs, using oligonucleotide primers and nucleic acid amplification, are provided. Reaction conditions that result in preferential decrease in cDNA synthesis of RNAs other than the small RNA molecules targeted for detection during miRNA tailing and reverse transcription reactions are described. Using these reaction conditions greater sensitivity and specificity of amplification of small RNAs including miRNAs is achieved. | 2010-05-06 |
20100112645 | Generation of modified polymerases for improved accuracy in single molecule sequencing - Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template. | 2010-05-06 |
20100112646 | ENZYMATIC HYDROLYSIS OF A CELLULOSE MATERIAL TREATED WITH AN IONIC LIQUID - A process for preparing glucose from a cellulose material, in which a cellulose-comprising starting material is provided and treated with a liquid treatment medium comprising an ionic liquid whose anions are selected from among polyatomic anions and the cellulose-comprising material which has been treated with the ionic liquid is subjected to an enzymatic hydrolysis, is described. | 2010-05-06 |
20100112647 | METHOD FOR PRODUCING AN ACIDIC SUBSTANCE HAVING A CARBOXYL GROUP - An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium. | 2010-05-06 |
20100112648 | (R)-HYDROXYNITRILE LYASE FROM BRASSICACEAE - The invention concerns a polypeptide which can be isolated from the Brassicaceae family and which has at least the activity of a hydroxynitrile lyase (HNL). The hydroxynitrile lyase of the invention is the first HNL from the Brassicaceae family. The plants ( | 2010-05-06 |
20100112649 | COMPOSITIONS, METHODS AND USES FOR GROWTH OF MICROORGANISMS AND PRODUCTION OF THEIR PRODUCTS - Embodiments of the present invention report compositions, methods and uses for growth and/or production of byproducts, products and/or co-products from algal cultures. Certain embodiments of the present invention report using wastewaters to grow microorganisms for producing one or more products, byproducts or co-products from the microorganisms. Other embodiments report compositions and methods for rapid growth of any photosynthetic organism in culture. | 2010-05-06 |
20100112650 | POWDERY LIPASE PREPARATION, METHOD FOR PRODUCING THE SAME AND USE THEREOF - The present invention discloses a powdery lipase preparation which is a granulated material comprising a lipase derived from | 2010-05-06 |
20100112651 | COMPOSITIONS AND METHODS FOR MANIPULATING CARBON FLUX IN CELLS - Nucleotide and protein sequences that encode enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as a | 2010-05-06 |
20100112652 | Method for Producing Lactic Acid By The Fermentation of a Self-Sufficient Medium Containing Green Cane Juice - The invention relates to a process for producing lactic acid by fermentation of a sugarcane extract by means of microorganisms belonging to the | 2010-05-06 |
20100112653 | Process of Producing a Fermentation Product - The present invention relates to processes for producing a fermentation product from starch-containing material comprising: (a) saccharifying starch-containing material with an alpha-amylase at a temperature below the initial gelatinization temperature of said starch-containing material; (b) fermenting using a fermenting organism, wherein a phytase is present during steps (a) and/or (b). | 2010-05-06 |
20100112654 | MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL - The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO. The invention additionally provides methods of using such microbial organisms to produce BDO. | 2010-05-06 |
20100112655 | ENHANCED PYRUVATE TO 2,3-BUTANEDIOL CONVERSION IN LACTIC ACID BACTERIA - A high flux of metabolites from pyruvate to 2,3-butanediol in | 2010-05-06 |
20100112656 | RE-ENGINEERING BACTERIA FOR ETHANOL PRODUCTION - The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed. | 2010-05-06 |
20100112657 | NUCLEIC ACIDS AND CONSTRUCTS FOR INCREASING GALACTOSE CATABOLISM AND METHODS THEREFOR - Provided are a recombinant gene associated with increased galactose catabolism, and a recombinant vector and microorganism including the gene. Also disclosed are a method of producing ethanol from a galactose-containing carbon source by culturing the microorganism including the gene in a galactose-containing carbon source such that ethanol is produced, and a method of screening a gene in yeast resulting in increased galactose catabolism when overexpressed. | 2010-05-06 |
20100112658 | Transformed Saccharomyces cerevisiae Engineered for Xylose Utilization - Recombinant | 2010-05-06 |
20100112659 | FERMENTATION PROCESS - A process for the biosymbiotic fermentation of one or more microbe species selected for production of bioactive molecules. These microbe species (bio-hosts) are fermented together with both botanical sources or bacterial, fungal or algal organisms (growth-symbiots) as promoter stimulants. In this manner yields of the bioactive molecules equivalent to those obtained through the biotechnological generation of expression vectors are possible. Furthermore, the invention contemplates biodegradation processes for the decomposition of the resulting fermentation biomass in such a way as to concentrate the bioactive molecules of interest in a precipitate or oleoresin form. | 2010-05-06 |
20100112660 | Method for Derivatization of Proteins Using Hydrostatic Pressure - The present invention provides an effective method for derivatization of proteins using hydrostatic pressure to reversibly perturb the native conformation of a protein such that a normally buried functional group on the protein, such as an amino acid residue, or a ligand or cofactor associated with the protein, is exposed and available for derivatization by a polymer molecule or a cytotoxic agent. The methods described herein do not require use of chaotropes, changes in pH, changes in temperature, or genetic modification of the native primary sequence of the protein and are applicable to substantially all proteins. | 2010-05-06 |
20100112661 | ONCOKINASE FUSION POLYPEPTIDES ASSOCIATED WITH HYPERPROLIFERATIVE AND RELATED DISORDERS, NUCLEIC ACIDS ENCODING THE SAME AND METHODS FOR DETECTING AND IDENTIFYING THE SAME - Oncokinase fusion polypeptides associated with hyperproliferative disorders and the polynucleotides encoding for such fusion polypeptides are provided. The fusion polypeptides have a C-terminal tyrosine kinase domain fused to an N-terminal domain that is not normally fused to the C-terminal tyrosine kinase domain and they possess constitutively activated tyrosine kinase activity. Also provided are methods for detecting and identifying the fusion polypeptides and polynucleotides and methods of diagnosing disease conditions associated with the fusion polypeptides and polynucleotides. In addition, screening assays for identifying agents useful for treating disease conditions associated with such fusion polypeptides and polynucleotides are provided. Furthermore, methods of treating disease conditions associated with the presence of the fusion polypeptides are provided. | 2010-05-06 |
20100112662 | MICROORGANISM FOR PRODUCING RECOMBINANT PIG LIVER ESTERASE - The invention relates to a method for the processing of reaction solutions, containing whole-cell catalysts, an aqueous component and an organic component, wherein the organic component contains the product to be enriched, with the following steps: a) adjustment of the pH value to less than 4; b) filtration of the reaction solution in the presence of a filter aid, preferably in the reaction solution; c) optionally: further enrichment and/or purification of the product contained in the organic component. | 2010-05-06 |
20100112663 | Enzymatic Treatment of Skin and Hide Degreasing - The present invention relates to a method of degreasing skins and hides with certain variants of | 2010-05-06 |
20100112664 | METHOD OF MAKING MACROPOROUS CATION EXCHANGE RESINS - Methods of making macroporous cation exchange resins are described. The macroporous cation exchange resins are in the form of particles such as beads that contain a hydrophilic, crosslinked, (meth)acrylic-type polymeric material. Additionally, methods of purifying a positively charged material using the macroporous cation exchange resins, methods of making chromatographic columns that contain the macroporous cation exchange resins, methods of making filter elements that contain the macroporous cation exchange resins, and methods of making porous composite materials that contain the macroporous cation exchange resins are described. | 2010-05-06 |
20100112665 | Crystallization and Structure Determination of Glycosylated Human Beta Secretase, an Enzyme Implicated in Alzheimer's Disease - An inhibitor bound form of human beta secretase, also known as memapsin 2 and BACE, particularly in a glycosylated form as expressed in Chinese hamster ovary (CHO), HEK293 cells, or in insect cells as part of a Baculovirus expression system has been crystallized, and the three dimensional x-ray crystal structure has been solved to 3.2 Å resolution. The x-ray crystal structure is useful for solving the structure of other molecules or molecular complexes, and designing inhibitors of human beta secretase activity. | 2010-05-06 |
20100112666 | Dehalogenases, Nucleic Acids Encoding Them and Methods for Making and Using Them - The invention relates to haloalkane dehalogenases and to polynucleotides encoding the haloalkane dehalogenases. In addition methods of designing new dehalogenases and method of use thereof are also provided. The dehalogenases have increased activity and stability at increased pH and temperature. | 2010-05-06 |
20100112667 | Microfluidic Biological Extraction Chip - A microfluidic cartridge for isolating biological molecules having a capture chamber containing functionalized solid supports maintained in a fluidized state provides reduced pressure drops and bubble formation during microfluidic extraction. The cartridge may include an electric field lysis chamber and/or a chemical lysis chamber. The electric-field lysis chamber may comprise an electrically insulating structure arranged between two opposing planar electrodes. | 2010-05-06 |
20100112668 | Method For Producing Factor G Derived From HorseShoe Crab - The invention provides a virus harboring a DNA encoding a subunit of | 2010-05-06 |
20100112669 | MDCK CELLS LINES SUPPORTING VIRAL GROWTH TO HIGH TITERS AND BIOREACTOR PROCESS USING THE SAME - The present invention relates to novel MDCK cells which can be to grow viruses, e.g., influenza viruses, in cell culture to higher titer than previously possible. The MDCK cells can be adapted to serum-free culture medium. The present invention further relates to cell culture compositions comprising the MDCK cells and cultivation methods for growing the MDCK cells. The present invention further relates to methods for producing influenza viruses in cell culture using the MDCK cells of the invention. | 2010-05-06 |
20100112671 | Host Cells for Production of Isoprenoid Compounds - Methods for synthesizing isopentenyl pyrophosphate are provided. A first method comprises introducing into a host microorganism a plurality of heterologous nucleic acid sequences, each coding for a different enzyme in the mevalonate pathway for producing isopentenyl pyrophosphate. A related method comprises introducing into a host microorganism an intermediate in the mevalonate pathway and at least one heterologous nucleic acid sequence, each sequence coding for an enzyme in the mevalonate pathway necessary for converting the intermediate into isopentenyl pyrophosphate. The invention also provides nucleic acid sequences, enzymes, expression vectors, and transformed host cells for carrying out the methods. | 2010-05-06 |
20100112672 | PRODUCTION OF ISOPRENOIDS AND ISOPRENOID PRECURSORS - The present invention provides genetically modified host cells and use of same for producing isoprenoid compounds. | 2010-05-06 |
20100112673 | DNA SEQUENCE ENCODING PENICILLIN ACYLASE, NOVEL RECOMBINANT DNA CONSTRUCTS AND RECOMBINANT MICROORGANISMS CARRYING THIS SEQUENCE - The invention consists in a nucleotide sequence having the size of (2646) bp, wherein the order of nucleotides is identical to the order of the nucleotide sequence encoding penicillin acylase from | 2010-05-06 |
20100112674 | MICROCIN H47 PLASMID SELECTION SYSTEM - The present invention relates generally to stabilized expression plasmid systems. The stabilized expression plasmid systems comprise an expression vector that includes a plasmid maintenance system (PMS) and, optionally, one or both of a polynucleotide encoding a selected antigen under control of a promoter, and a polynucleotide encoding a selectable marker under control of a promoter. The use of the mchI protein as a selectable marker is found in preferred embodiments of the invention. | 2010-05-06 |
20100112675 | MULTI-STAGED COMPOSTING - In various embodiments, the invention provides staged processes, or systems, for composting organic materials, such as waste plant and animal matter. In one aspect, the invention involves the staged passage of organic material through alternative composting ecologies, to optimize the diversity of decay organisms that may work on the organic materials. In some embodiments, a robust, well mixed thermophilic environment is created for primary stage aerobic composting. The primary compost produced by this stage may be transferred to an alternative stratified composting ecology, in which the secondary compost descends over time from a relatively stable layer of residual thermophilic aerobic composting to underlying layers that involve non-thermophilic aerobic degradation of the organic material. | 2010-05-06 |
20100112676 | ODOR CONTROL METHODS AND COMPOSITIONS - This invention is directed generally to methods of controlling the odor of a biological material, and more particularly to methods comprising providing the biological material with an Fe(III)-reducing bacteria and a source of Fe(III). This invention also is directed generally to compositions and kits for controlling the odor of a biological material. | 2010-05-06 |
20100112677 | STERILE ENVIRONMENT MAINTAINING APPARATUS - Provided is a sterile environment maintaining apparatus capable of sterilizing a sterile room in a shorter time than conventional ones. | 2010-05-06 |
20100112678 | ANALYTICAL TEST STRIP WITH MINIMAL FILL-ERROR SAMPLE VIEWING WINDOW - An electrochemical-based analytical test strip includes an electrically-insulating substrate, a patterned conductive layer disposed over the electrically-insulating substrate, a patterned insulating layer disposed over the patterned conductive layer, an enzymatic reagent layer disposed over the patterned conductive layer, a patterned adhesive layer disposed above the enzymatic reagent layer and a top layer disposed over the enzymatic reagent layer. In addition, the test strip has a sample-receiving chamber defined by the electrically-insulating substrate, the patterned conductive layer, the patterned insulating layer, the enzymatic reagent layer, the patterned adhesive layer and the top layer. The sample receiving chamber of the test strip has a working portion and a non-working portion and the top layer has a first portion and an opaque second portion. The first portion is configured such that a user can view the working portion of the sample-receiving chamber through the first portion of the top layer, while the opaque second portion is configured to preclude a user from viewing the non-working portion of the sample-receiving chamber. | 2010-05-06 |
20100112679 | Method And Apparatus For Syringe-Based Sample Introduction Within A Flow Cytometer - An apparatus for introducing a specimen into a flow cytometer comprises: a syringe having a hollow barrel containing the specimen, a plunger partially within the barrel and a needle that extends into a volume of a nozzle of the flow cytometer; a one-way port in the nozzle forming a seal against the needle; a mounting platform coupled to both the syringe and to the flow cytometer; and a syringe pump coupled to the plunger, the syringe pump comprising a motor, a drive mechanism coupled to the motor; and a clamping mechanism coupled to the drive mechanism, wherein the motor operates the drive mechanism so as to cause the clamping mechanism to depress the plunger into the barrel. | 2010-05-06 |
20100112680 | INDICATOR SYSTEM FOR DETERMINING ANALYTE CONCENTRATION - A method for quantitatively sensing, using an indicator system based on diffusion in space and time of a reaction front, for determining and reporting the prevailing concentration or exposure history of an analyte in food, beverage, and pharmaceutical monitoring for the state of quality, for ripeness indication in fruit, for monitoring environments for concentrations of sanitisers, pollutants and nutrients, for monitoring the residual life of filters, and for monitoring stream flows. | 2010-05-06 |
20100112681 | MICROCHIP FLUID CONTROL MECHANISM - Compressed gas is supplied from above to a specimen vessel having an open top. Transfer channels to reaction vessels are provided under a microchip and transfer channels extending from the reaction vessels are provided on the microchip. Transfer element is provided outside the microchip so as to supply the compressed gas from a member (cover) holding the microchip. | 2010-05-06 |
20100112682 | TOTAL BACTERIA MONITORING SYSTEM - A system for measuring total microbiological content in an aqueous medium by adding a fluorescent dye to the aqueous medium, measuring the fluorescent signal in the aqueous medium to obtain a baseline fluorescent signal, releasing intracellular content of the microbiological matter into the aqueous medium by lysing the microbiological matter. The system then measures the fluorescent signal in the aqueous medium with the released intracellular content of the microbiological matter to obtain a second fluorescent signal. Next, the system subtracts the baseline signal from the second fluorescent signal to obtain a net fluorescent signal and equates the net fluorescent signal with a microbiological content. | 2010-05-06 |
20100112683 | Thermal Cycler for Automatic Performance of the Polymerase Chain Reaction with Close Temperature Control - A thermal cycler for automatic performance of the polymerase chain reaction is provided. The thermal cycler comprises a heater control that provides close temperature control of the reaction. | 2010-05-06 |
20100112684 | CELL CULTURE DISH FOR THE EMBRYOID BODY FORMATION FROM EMBRYONIC STEM CELLS - The present invention relates to a cell culture dish for the erabryoid body formation from embryonic stem cells, which facilitates efficient and stable differentiation of embryonic stem cells by forming embryoid body in grooves on the bottom of a support adhered on the back of the lid for the culture dish by hanging drop culture. | 2010-05-06 |
20100112685 | Thymidine Kinase Mutants and Fusion Proteins Having Thymidine Kinase and Guanylate Kinase Activities - The present invention provides isolated nucleic acid molecules encoding a Herpesviridae thymidine kinase enzyme comprising one or more mutations, at least one of the mutations encoding an amino acid substitution located toward the N-terminus from a DRH nucleoside binding site which increases a biological activity of the thymidine kinase, as compared to unmutated thymidine kinase. Such mutations include amino acid substitutions within a Q substrate binding domain which increases a biological activity of the thymidine kinase, as compared to unmutated thymidine kinase. Within a further aspect, fusion proteins are provided which have both guanylate kinase and thymidine kinase biological properties. Also provided are vectors suitable for expressing such DNA molecules, as well as methods for utilizing such vectors. | 2010-05-06 |
20100112686 | SHORT HAIRPIN RNAS FOR INHIBITION OF GENE EXPRESSION - Methods, compositions, and kits that include small hairpin RNA (shRNA) useful for inhibition of gene expression, such as viral-mediated gene expression, are described. | 2010-05-06 |
20100112687 | NUCLEIC ACID COMPOUNDS FOR INHIBITING ERBB FAMILY GENE EXPRESSION AND USES THEREOF - The present disclosure provides meroduplex ribonucleic acid molecules (mdRNA) capable of decreasing or silencing one or more ERBB family gene expression. An mdRNA of this disclosure comprises at least three strands that combine to form at least two non-overlapping double-stranded regions separated by a nick or gap wherein one strand is complementary to one or more ERBB family mRNA. In addition, the meroduplex may have at least one uridine substituted with a 5-methyluridine and optionally other modifications or combinations thereof. Also provided are methods of decreasing expression of one or more ERBB family gene in a cell or in a subject to treat one or more ERBB family-related disease. | 2010-05-06 |
20100112688 | Screening Methods for Compounds that Modulate the Activity of G-Protein Coupled Receptors - The present invention relates to a screening system for modulators of GPCRs. Further it relates to recombinant vector systems for the heterologous expression of heterodimeric g-protein coupled receptors (GPCRs) in eucaryotic host cells. Preferably the functional expression of engineered GPCRs for the perception of sweet and L-amino acid taste or more preferably the use of said receptors for the identification of functional ligands is also encompassed. | 2010-05-06 |
20100112689 | METHOD OF OBTAINING VIABLE HUMAN CELLS, INCLUDING HEPATIC STEM/PROGENITOR CELLS - The present invention is directed toward a method for obtaining from whole liver or a resection thereof a population of cells comprising viable, functional liver cells enriched in hepatocytes and hepatocyte stem/progenitor cells, compositions thereof, and uses therefore. Compositions include a composition of liver cells enriched in hepatocytes and hepatocyte stem/progenitor cells and a pharmaceutical composition thereof. Uses include treatment of liver diseases, regeneration of liver, toxicity testing, and liver assist devices. | 2010-05-06 |
20100112690 | INSERT DEVICE FOR MULTIWELL PLATE - An assembly comprises a multiwell test plate having a plurality of wells having an opening and a base surface, an insert plate having a plurality of inserts positioned to align with a corresponding one of the wells whereby the insert plate can be nested with the multiwell plate. Each of the inserts includes a supply port arranged for flow communication with a supply source, an exhaust port, a bottom portion having a plurality of channels extending between the supply port and the exhaust port, and a gas permeable membrane covering the bottom portion. Each of the inserts is sized to position the gas permeable membrane a desired distance from the base surface of the multiwell test plate when the multiwell test plate and the insert plate are coupled to one another. | 2010-05-06 |
20100112691 | Stem Cell Aggregate Suspension Compositions and Methods of Differentiation Thereof - The present invention relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation thereof. | 2010-05-06 |
20100112692 | Differentiation of Human Embryonic Stem Cells - The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides a method to increase the expression of markers associated with the pancreatic endocrine lineage. | 2010-05-06 |
20100112693 | Differentiation of Human Embryonic Stem Cells - The present invention provides a method for increasing the expression of MAFA in cells expressing markers characteristic of the pancreatic endocrine lineage comprising the steps of culturing the cells expressing markers characteristic of the pancreatic endocrine lineage in medium comprising a sufficient amount of a cyclin-dependant kinase inhibitor to cause an increase in expression of MAFA. | 2010-05-06 |
20100112694 | Dedifferentiation of Adult Mammalian Cardiomyocytes into Cardiac Stem Cells - Dedifferentiation is a mechanism whereby specialized cells regain properties of their ancestors, including, in the extreme, stemness. We found that highly-purified cardiomyocytes isolated from adult mammalian hearts dedifferentiated rapidly when cultured in mitogen-rich medium. Such myocytes reentered the cell cycle and proliferated, expressing stem cell surface markers such as c-kit and early cardiac transcription factors including GATA and NKx2.5. These myocyte-derived cells (MDC) were capable of re-differentiating into myocytes and endothelial cells. Contrary to prevailing dogma, cardiomyocyte dedifferentiation yields proliferative cells expressing stem cell markers and capable of multilineage differentiation. Cardiomyocyte dedifferentiation is a potential source of endogenous stem cells in the adult heart. | 2010-05-06 |
20100112695 | Apparatus And Methods For Processing Tissue to Release Cells - An apparatus and methods for processing tissue to release biological material including cells are disclosed. | 2010-05-06 |
20100112696 | Apparatus And Methods For Processing Tissue To Release Cells - An apparatus and methods for processing tissue to release biological material including cells are disclosed. | 2010-05-06 |
20100112697 | PROCESS FOR THE HIGH-PURITY ISOLATION OF MESENCHYMAL STEM CELLS DERIVED FROM PLACENTAL CHORIONIC PLATE MEMBRANE - The present invention provides a method for isolating mesenchymal stem cells derived from a placental chorionic plate membrane, the method including: (a) harvesting a chorionic plate membrane from a detached placenta; (b) harvesting cells present in the chorionic plate membrane obtained in step (a) by scraping; (c) adding a solution containing trypsin and ethylenediaminetetraacetate to the cells obtained in step (b) to perform an enzymatic reaction and adding a fetal bovine serum thereto to terminate the enzymatic reaction; and (d) centrifuging the reaction solution obtained in step (c) and culturing the obtained cells in a medium containing a fetal bovine serum and an antibiotic. | 2010-05-06 |
20100112698 | Device for Trapping Extraneous Gas - A device for trapping extraneous gas or bubbles is disclosed. The device is defined as having a gas collection area in a tapered neck portion such that when used as a cell culturing apparatus, extraneous gas or bubbles are trapped and isolated from the cell growth areas where cells or media reside. | 2010-05-06 |
20100112699 | POROUS COMPOSITE BIOMATERIALS AND PRODUCTION METHOD OF THE SAME - The invention discloses a porous composite biomaterial comprising of poly(γ-glutamic acid)-g-chondroitin sulfate (γ-PGA-g-CS) copolymer and poly(ε-caprolactone). The composite biomaterial provides a three-dimensional microenvironment for using as a scaffold for tissue engineering and for supporting the attachment and proliferation of cells. The invention also discloses a method of producing a porous composite biomaterial. | 2010-05-06 |
20100112700 | LARGE SCALE DISPOSABLE BIOREACTOR - A reusable, disposable device for culturing plant tissues or cells including a non-rigid container having dimensions and gas exchange ports designed for maintaining oxygen saturation and shear forces suitable for culturing plant tissue or cells in 400 liters or more of culture medium is provided. Also provided are methods for producing a catalytically active human recombinant protein in a plant cell, using the disposable device of one of the embodiments of the instant specification. | 2010-05-06 |
20100112701 | REDUCTASE, GENE THEREOF AND METHOD OF USING THE SAME - The present invention relates to a protein which can asymmetrically reduce an ortho-substituted phenylglyoxalic acid compound to produce an aide or ester compound of corresponding optically-active ortho-substituted mandelic acid compound with a good optical yield, a DNA encoding the protein, a process for producing the protein from the DNA, and a process for asymmetrically reducing an ortho-substituted phenylglyoxalic acid compound to produce a corresponding optically-active ortho-substituted mandelic acid compound. | 2010-05-06 |
20100112702 | Method designed to divert glucose away from the glycolytic pathway - A method to divert glucose away from glycolysis is described. The method is based on a planned “glucose sink” by introduction, into tumor cells, of nucleotide sequences from known genes whose products are comprised in lactose synthase enzymatic complex. The method is a process designed to correct, replenish or de novo induce lactose synthesis in cancer cells planned to be achieved by insertion of the cDNA of missing protein products capable of lactose synthesis by an expression vector under a strong promoter/enhancer. Intended introduction of the said enzyme expression system by a vector into the cancer cells is by transfection in culture for research. For therapeutic use introduction in vivo is intended by transvection/infection via nanotechnological delivery or viral delivery when delivery systems will be authorized. The best embodiment of this method is in medicine/veterinary where it may require a diagnostic step that defines in individual tumors, especially in mammary tumors, the quantitative and qualitative expression of endogenous genes that enable lactose synthesis, including mainly β4GalT1, α-lactalbumin, and galactose-generating glucose-epimerase, originating preferably from the same species in the case of human subjects. Expression deficiency, deletions, mutations or absence of one of these genes is a guide for induction, correction (if mutated) or replenishment. | 2010-05-06 |
20100112703 | Sample processing system and controlling method of the same - A sample processing system is disclosed that comprises: a transportation apparatus configured to convey a sample container; a first analyzer, arranged along the transportation apparatus, configured to measure a sample accommodated in a sample container conveyed by the transportation apparatus; a second analyzer, arranged along the transportation apparatus, configured to measure a sample accommodated in a sample container conveyed by the transportation apparatus; and a transportation controller configured to determine an analyzer to which a sample container is conveyed, and to control the transportation apparatus to convey the sample container to the determined analyzer, wherein: the first analyzer is configured to conduct primary measurement; and the second analyzer is configured to conduct both primary measurement and review measurement. | 2010-05-06 |
20100112704 | ANALYZER AND ITS ABNORMALITY COPING METHOD - An analyzer includes plural stirrers that have plural sound-wave generating units, which are provided on respective vessels holding liquid and generate sound wave toward the liquid, and drive units, which drive the plurality of sound-wave generating units, and that stir the liquid using sound wave generated by the sound-wave generating units; a power detecting unit that detects traveling-wave power output from the drive unit and reflected-wave power reflected from the sound-wave generating unit; and a control unit that determines whether an abnormality occurs in each of the stirrers on the sound-wave generating unit side or on the drive unit side in each of the stirrers based on a reflectivity of the power, and controls continuation or suspension of an analytical work based on a result of the determination. | 2010-05-06 |
20100112705 | POLYNUCLEOTIDES FOR USE AS TAGS AND TAG COMPLEMENTS, MANUFACTURE AND USE THEREOF - A family of minimally cross-hybridizing nucleotide sequences, methods of use, etc. A specific family of 210 24mers is described. | 2010-05-06 |
20100112706 | METHOD FOR ANALYZING STRUCTURE AND PURITY OF SEROTONIN TRANSPORTER IMAGING AGENT [123I] ADAM AND PRECURSOR SnADAM - An analytical technique for determining the structures of serotonin transporter (SERT) imaging agent [ | 2010-05-06 |
20100112707 | Biosensor - The invention relates to a biosensor solution in which the biosensor comprises at least one sol-gel response region pattern doped with a biological signature molecule and printed on the biosensor, and at least one micro-channel for transporting material to said at least one sol-gel response region pattern. | 2010-05-06 |
20100112708 | Methods and Mixtures Pertaining to Analyte Determination Using Electrophilic Labeling Reagents - This invention pertains to methods, mixtures, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes. | 2010-05-06 |
20100112709 | HIGHLY SENSITIVE METHOD FOR DETECTING PROTEIN IN FOOD - A method for accurate and precise measurement of target proteins such as food allergen proteins in the specific foods is provided. The method is a method for immunological measurement of a food allergen protein in a processed food using an antibody against the food allergen protein, comprising adding animal tropomyosin to an assay solution upon measurement. | 2010-05-06 |
20100112710 | METHODS AND COMPUTER SYSTEMS FOR IDENTIFYING TARGET-SPECIFIC SEQUENCES FOR USE IN NANOREPORTERS - The present invention relates to compositions and methods for detection and quantification of individual target molecules in biomolecular samples. In particular, the invention relates to coded, labeled probes that are capable of binding to and identifying target molecules based on the probes' label codes. Methods, computers, and computer program products for identifying target-specific sequences for inclusion in the probes are also provided, as are methods of making and using such probes. The probes can be used in diagnostic, prognostic, quality control and screening applications. | 2010-05-06 |
20100112711 | METHOD OF DETECTING AMPLIFICATION OR DELETION IN GENOMIC DNA FRAGMENT - [Problems] To provide a CGH method, in particular a CGH microarray method, enabling detection at an elevated accuracy and elevated sensitivity. | 2010-05-06 |
20100112712 | Modified Oligonucleotides and Applications Thereof - Disclosed, among other things, are primers containing certain modified nucleobases in the 3′ terminal region of the primers that provide reduced formation of primer-dimers during amplification reactions, and various methods of use thereof. | 2010-05-06 |
20100112713 | Methods For Detecting Colorectal Diseases And Disorders - The present invention relates to methods and compositions for the detection of biomarkers associated with colorectal diseases and disorders. In preferred embodiments, said colorectal disease is colorectal cancer. In some embodiments, the invention relates to the detection of said biomarkers using non-invasive methods. In further embodiments, the invention relates to the isolation and evaluation of biomarkers residing in feces from a subject at risk for or exhibiting symptoms associated with a colorectal disease or disorder. In still further embodiments, said biomarkers include exfoliated colonocytes. In additional embodiments, mRNA transcripts isolated from said colonocytes and associated with said colorectal diseases and disorders are quantified. | 2010-05-06 |
20100112714 | BIOCHIP - The present invention relates to a biochip for nucleic acid hybridization. The biochip of the present invention comprises a hybridization chamber which is in the form of a cavity, a porous membrane pressed in the hybridization chamber; and at least one first circulation hole and at least one second circulation hole which are communicated with the hybridization chamber so that the reaction solution flows in the at least one first circulation hole and flows out the at least one second circulation hole through the pores of the porous membrane. The hybridization reaction area is increased by flowing the reaction solution through the pores of the membrane, which enable the reaction sensitivity to be increased. The diffusion distance for the reaction molecules is decreased due to the limited inside space of the membrane, and thereby the hybridization time is shortened. | 2010-05-06 |
20100112715 | DETECTION OF EXPLOSIVES, TOXINS, AND OTHER COMPOSITIONS - The present invention generally relates to methods for modulating the optical properties of a luminescent polymer via interaction with a species (e.g., an analyte). In some cases, the present invention provides methods for determination of an analyte by monitoring a change in an optical signal of a luminescent polymer upon exposure to an analyte. Methods of the present invention may be useful for the vapor phase detection of analytes such as explosives and toxins. The present invention also provides methods for increasing the luminescence intensity of a polymer, such as a polymer that has been photobleached, by exposing the luminescent polymer to a species such as a reducing agent. | 2010-05-06 |
20100112716 | SYNTHESIS OF NANOASSEMBLIES CONTAINING LUMINESCENT QUANTUM DOTS AND MAGNETIC NANOPARTICLES - Negatively charged luminescent CdSe—ZnS quantum dots (QDs) were successfully incorporated into novel luminescent glyconanospheres averaging around 190 nm in diameter through electrostatic interactions with carboxymethyldextran (CM-dextran) and polylysine. The glyconanospheres preferably contain as well carboxyl-modified iron oxide nanocrystals. In addition to electrostatic attraction between the negatively charged dextran, the negatively charged CdSe—ZnS QDs (and negatively charged iron oxide nanocrystals, if present), and the positively charged polylysine, covalent amide bonds were introduced to cross link the QDs (and negatively charged iron oxide nanocrystals, if present) with the polysaccharide matrix to further stabilize the glyconanospheres. The dextran residues on the surface of the nanospheres show high affinity toward the glucose binding protein-Concanavalin A (Con A). As a result, these luminescent CdSe—ZnS QD incorporated glyconanospheres are a useful tool for studying carbohydrate-protein interactions that are critical steps in bacterial and viral infection. | 2010-05-06 |
20100112717 | Method for Providing a Dried Reagent in a Microfluidic System and Microfluidic System - A method for providing a dried reagent in a microfluidic system is provided, the method comprising the following steps: providing a microfluidic system having a microfluidic structure, introducing a flowable carrier medium containing a reagent in the microfluidic structure, and drying the reagent in the microfluidic structure by lyophilization. | 2010-05-06 |
20100112718 | Device For Receiving A Liquid And Also Device For Applying Liquids On Sample Carriers And Method For This Purpose - Methods and apparatus providing for receiving liquids and for applying liquids on sample carriers. | 2010-05-06 |
20100112719 | ELECTRONIC SIGNAL AMPLIFICATION IN FIELD EFFECT DEVICE BASED CHEMICAL SENSORS - Briefly, disclosed is a method and apparatus for detecting an analyte wherein an enhanced charge marker may enhance steric, electrostatic, optic and/or mechanical changes associated with a recognition event between an analyte and a probe. | 2010-05-06 |