17th week of 2011 patent applcation highlights part 38 |
Patent application number | Title | Published |
20110097704 | COMPOSITIONS FOR USE IN IDENTIFICATION OF PICORNAVIRUSES - The present invention provides oligonucleotide primers, compositions, and kits containing the same for rapid identification of viruses which are members of the Picornaviridae family by amplification of a segment of viral nucleic acid followed by molecular mass analysis. | 2011-04-28 |
20110097705 | Surface-assisted hemagglutination and hemagglutination inhibition assays - Hemagglutination (HA) and hemagglutination inhibition (HAI) functional assays remain important instruments of analysis of virus-cell interaction and protecting efficacy of virus-specific antibodies and sera. However, they demonstrate limited sensitivity towards many viruses, and require significant volumes of viruses, erythrocytes, sera, and antibodies. The present invention comprises new and significantly more sensitive versions of the HA and HAI assays based on observing agglutination on activated surfaces of specifically opsonized plates and ELISA plates rather than in solution. A version of the new assay that uses ELISA plates additionally allows characterizing the affinity of functional antibodies in the tested sera and fluids, which is not possible in the classical HAI assay. The methods of the present invention can also be used to improve the sensitivity of agglutination methods based on latex beads and to develop agglutination methods using target cells other than erythrocytes. | 2011-04-28 |
20110097706 | MICRO-REACTOR FOR OBSERVING PARTICLES IN A FLUID - The invention relates to a micro-reactor for observing small particles, cells, bacteria, viruses or protein molecules in a fluid. The micro-reactor shows a first channel formed between two layers for containing the fluid, with an inlet and an outlet, the two layers separated by a first distance. A likewise second channel with an inlet and an outlet is placed adjacent to the first channel. A gap connects the first channel and the second channel, at the gap at least one layer showing a window transparent to the method of inspection and at the window the two layers being separated by a very small distance of, for example, 1 μm or less. | 2011-04-28 |
20110097707 | RNAi Agents Comprising Universal Nucleobases - One aspect of the present invention relates to an oligonucleotide agent comprising at least one universal nucleobase. In certain embodiments, the universal nucleobase is difluorotolyl, nitroindolyl, nitropyrrolyl, or nitroimidazolyl. In a preferred embodiment, the universal nucleobase is difluorotolyl. In certain embodiments, the oligonucleotide is double-stranded. In certain embodiments, the oligonucleotide is single-stranded. Another aspect of the present invention relates to a method of altering the expression level of a target in the presence of target sequence polymorphism. In a preferred embodiment, the oligonucleotide agent alters the expression of different alleles of a gene. In another preferred embodiment, the oligonucleotide agent alters the expression level of two or more genes. In another embodiment, the oligonucleotide agent alters the expression level of a viral gene from different strains of the virus. In another embodiment, the oligonucleotide agent alters the expression level of genes from different species. | 2011-04-28 |
20110097708 | ASSESSMENT OF HUMAN PAPILLOMA VIRUS-RELATED DISEASE - This invention provides novel methods for assessing HPV infection. Gene expression levels are used to assess the progression of HPV infection from benign to malignant growth. Also provided are kits for carrying out the methods of this invention. | 2011-04-28 |
20110097709 | Method for modifying a nucleic acid - Disclosed are methods for increasing the rate at which a nucleic acid can be translated by modifying the polynucleotide such that the rate at which the polynucleotide is translated is altered. Also disclosed are methods for modulating the translation of an mRNA by adding a nucleic acid that increases translation of the mRNA | 2011-04-28 |
20110097710 | Methods for detecting atrial fibrillation and related conditions - Methods for detecting disorders associated with atrial fibrillation, and, thus, at risk for stroke and/or heart failure) in a subject based on a monitoring plasma levels of apelin are provided. Diagnostic compositions for the detection of such disorders are additionally provided. | 2011-04-28 |
20110097711 | METHOD OF JUDGING INFLAMMATORY DISEASE BY USING SINGLE NUCLEOTDIE POLYMORPHISM - An object of the present invention is to identify a novel single nucleotide polymorphism (SNP) associated with the onset and the advancement of inflammatory diseases such as myocardial infarction. The present invention provides a method for judging an inflammatory disease which comprises detecting at least 1 type of genetic polymorphism existing in at least one gene selected from the group consisting of the LBP-32 gene, the TSBP gene, and the WAP gene. | 2011-04-28 |
20110097712 | METHOD FOR DETECTING AND QUANTIFYING RARE MUTATIONS/POLYMORPHISMS - The present invention is directed to a method for detecting and quantifying rare mutations in a nucleic acid sample. The nucleic acid molecules under investigation can be either DNA or RNA. The rare mutation can be any type of functional or non-functional nucleic acid change or mutation, such as deletion, insertion, translocation, inversion, one or more base substitution or polymorphism. Therefore, the methods of the present invention are useful in detection of rare mutations in, for example, diagnostic, prognostic and follow-up applications, when the targets are rare known nucleic acid variants mixed in with the wildtype or the more common nucleic acid variant(s). | 2011-04-28 |
20110097713 | Compositions and methods for pharmacogenomic screening of CYP2C9 and VKORC1 - Compositions and methods for determining an optimal dose of a medication for a subject are described that include determining the subject's genotype for the CYP2C9 and VKORC1 genes and determining the dose of the medication based on the genotype. Articles of manufacture also are provided that include polynucleotides for genotyping. | 2011-04-28 |
20110097714 | AMPLIFICATION METHOD OF METHYLATED OR UNMETHYLATED NUCLEIC ACID - The object of the present invention is to provide a gene amplification method, wherein the method can amplify both methylated and unmethylated nucleic acids present in a biological sample, and further regulate the amplification ratio of the methylated and/or unmethylated nucleic acid as needed. Such objects can be solved by an amplification method using a nonspecific primer which can hybridize both with methylated and unmethylated nucleic acids and a specific primer which specifically hybridizes with either methylated or unmethylated nucleic acid, and further by an amplification method which can change the amplification ratio of methylated or unmethylated nucleic acid by changing the mixing rate of these primers. | 2011-04-28 |
20110097715 | METHOD FOR PURIFYING OR DETECTING A TARGET PROTEIN - A method for purifying or detecting a target protein present in a solution, includes, before carrying out the actual detection or purification step, a step of contacting the solution with an aptamer binding specifically to the target protein, where the aptamer does not bind to a protein homologous to the target protein that could also be present in the solution. | 2011-04-28 |
20110097716 | Methods for Detecting Oligonucleotides - The invention provides methods and compositions for detecting and/or quantifying nucleic acid oligonucleotides. These methods and compositions are useful for detecting and quantifying diagnostic and/or therapeutic synthetic target oligonucleotides, such as aptamers, RNAi, siRNA, antisense oligonucleotides or ribozymes in a biological sample. | 2011-04-28 |
20110097717 | Gene Expression Profiling For Identification of Cancer - A method is provided for determining whether an individual has a particular cancer based on a sample from the subject, wherein the sample provides a source of RNAs. The method includes using amplification for measuring the amount of RNA corresponding to at least 1 constituent from Tables A-C. | 2011-04-28 |
20110097718 | MS-COMPATIBLE NONIONIC OR ZWITTERIONIC SURFACTANTS IN FREE-FLOW ELECTROPHORESIS - The invention relates to the use of MS compatible surfactants in free-flow electrophoretic methods, which allow the separation of analytes with differentiated electrophoretic mobility. The surfactant is preferably a cleavable surfactant, such as PPS. | 2011-04-28 |
20110097719 | Neprilysin Gene Polymorphism and Amyloid Beta Plaques in Traumatic Brain Injury - The invention relates to methods of diagnosing risk of amyloid β deposition following traumatic brain injury. More particularly, the invention relates to the discovery of a specific single nucleotide polymorphism in the neprilysin gene that is linked to an increased risk of amyloid β deposition after traumatic brain injury | 2011-04-28 |
20110097720 | SCREENING METHOD FOR SELECTED AMINO LIPID-CONTAINING COMPOSITIONS - The invention features a method of identifying therapeutically relevant compositions which include a therapeutic agent and 2,2-Dilinoley 1-4-dimethylaminomethyl-[1,3]-dioxolane by screening for an effect of the agent on the liver of a model subject. | 2011-04-28 |
20110097721 | IN VIVO GENE SENSORS - Described are methods and compositions for the detection of target genes. The inventors have developed a synthetic nucleic acid sensor-effector gene circuit. In cells without a target gene, the circuit suppresses e.g., effector production, but in the presence of the target gene the suppression is subject to competition, such that the synthetic sensor is de-repressed and permits expression of the effector gene. The methods and compositions described further permit the selective expression of an effector gene in those cells expressing the target gene. In this manner, cells expressing a target gene can be selectively targeted for treatment or elimination. In certain aspects, the methods and compositions described permit the selective expression of an agent such as a therapeutic gene product, in a specifically targeted population of cells in an organism. | 2011-04-28 |
20110097722 | Cold Shock Protein Compositions and Methods and Kits for the Use Thereof - The present invention provides cold shock protein-containing compositions for improved DNA synthesis reactions with improved reactivity, methods for synthesizing DNA using such compositions, kits for use in such methods, and DNA compositions yielded by such methods. The present invention further provides cold shock protein-containing compositions for the identification of endoribonuclease cleavage sites, methods for identifying endoribonuclease cleavage sites using such compositions, and kits for use in such methods. | 2011-04-28 |
20110097723 | Methods and reagents for analyte detection - The present invention relates to chemiluminescent method and regent to detect analyte. One aspect of the current invention relates to using chemiluminescent and fluorescent molecule/enzyme coupled with analyte binding molecules to detect specific analyte molecules. Another aspect of the current invention is to use gold nanoparticle triggered chemiluminescent reaction to detect analyte. | 2011-04-28 |
20110097724 | Detection of Head and Neck Cancer Using Hypermethylated Gene Detection - Methods and kits for detection of a cell proliferative disorder, such as head and neck cancer are provided utilizing analysis of the methylation state of targeted genes or regulatory regions of genes in a saliva or serum sample are described. The presence of hypermethylation of the genes or their regulatory regions is indicative of the presence, or a stronger possibility of recurrence and or a poorer prognosis in subjects with cancer. | 2011-04-28 |
20110097725 | RAPID DETECTION OF MYCOBACTERIA - The invention provides a method for detecting a | 2011-04-28 |
20110097726 | REGENERATION AND NEOGENESIS OF RETINAL PHOTORECEPTOR CELL USING OTX2 GENE - The present invention provides a medicine, comprising (a) an Otx2 protein or its partial peptide, or a salt thereof, or (b) a DNA or an RNA encoding an Otx2 protein or its partial peptide. The present medicine is useful as an agent for preventing, treating or suppressing progression of a retinal disease including retinal degeneration. In addition, the present medicine is useful, for example, as an agent for inducing differentiation from a retinal stem cell into a retinal photoreceptor cell, in the transplantation of a cell into the retina of patients suffering from retinal diseases. | 2011-04-28 |
20110097727 | DNA MARKERS FOR MANAGEMENT OF CANCER - A method is provided for assessing allelic losses and hypermethylation of genes in CpG tumor promotor region on specific chromosomal regions in cancer patients, including melanoma, neuroblastoma breast, colorectal, and prostate cancer patients. The method relies on the evidence that free DNA and hypermethylation of genes in CpG tumor promotor region may be identified in the bone marrow, serum, plasma, and tumor tissue samples of cancer patients. Methods of melanoma, neuroblastoma, colorectal cancer, breast cancer and prostate cancer detection, staging, and prognosis are also provided. | 2011-04-28 |
20110097728 | MATERIALS AND METHOD FOR ASSAYING FOR METHYLATION OF CpG ISLANDS ASSOCIATED WITH GENES IN THE EVALUATION OF CANCER - Provided are methods, reagents, and kits for evaluating cancer, such as prostate cancer, in a subject. Disclosed methods of evaluating cancer include methods of diagnosing cancer, methods of prognosticating cancer and methods of assessing the efficacy of cancer treatment. The methods include assaying a biological sample for methylation of a CpG island associated with specified genes. Provided reagents and kits include primers suitable for amplifying at least a portion of a target CpG islands associated with specified genes. | 2011-04-28 |
20110097729 | APPARATUS AND METHOD FOR SEQUENCING NUCLEIC ACID MOLECULES - The sequence of a target polynucleotide can be determined by:
| 2011-04-28 |
20110097730 | Recombinant Sars-Cov nsp12 and the Use of Thereof and the Method for Producing it - The present invention relates to a recombinant severe acute respiratory syndrome coronavirus (SARS-CoV) non-structural protein (nsp) 12 with an RNA polymerase activity, its expression vector, its preparation method, and its use. According to the present invention, a soluble recombinant SARS-CoV nsp12 with an RdRp activity of initiating SARS-CoV genome synthesis can be over-expressed in the transformed host cells, and conveniently purified with high purity. An in vitro replication system important for studying SARS-CoV replication can be established with the purified recombinant SARS-CoV nsp12. SARS-CoV nsp12 produced by the present invention can also be used as a target for the development of anti-viral agents against SARS-CoV. In addition, materials inhibiting RNA-dependent RNA polymerase (RdRp) activity of nsp12 can be screened efficiently according to the present invention as the optimal conditions for the RdRp assay with SARS-CoV nsp12 were found. | 2011-04-28 |
20110097731 | NOVEL MITOCHONDRIAL DYE - The present invention relates to the use of single-barrel genetically encoded GFP-based calcium indicator as an intramitochondrial dye and to nucleic acid molecules coding for said indicators, as well as to methods using said indicators. Examples of single-barrel genetically encoded GFP-based calcium indicator is a GCaMP, Case16 and/or Case12. In a particular embodiment, the single-barrel genetically encoded GFP-based calcium indicator is GCaMP2 or Case16. | 2011-04-28 |
20110097732 | NOVEL BIOMARKER FOR THE PROGNOSIS OF BREAST CANCER - The present invention relates to methods of determining the predilection for survival for an individual having breast cancer comprising: obtaining a breast tissue sample from said individual, measuring the amount of Wrap53 in the nucleus of cells in said breast tissue sample, and/or measuring the amount of Wrap53 in the cytoplasm of cells in said breast tissue sample, and wherein both the nuclear levels, the cytoplasmic levels and/or the ratio between the nuclear and cytoplasmic levels of Wrap53 may be used alone or in combination in breast cancer prognosis. The invention furthermore relates to antibodies and kits relating to Wrap53. | 2011-04-28 |
20110097733 | PROCESS FOR THE PRODUCTION OF A HYBRIDOMA AND ANTIBODY OBTAINED THEREFROM, ABLE TO RECOGNIZE MORE THAN ONE VITAMIN D METABOLITE - The invention concerns a process for the production of a hybridoma, and of a monoclonal antibody or fragments thereof able to recognize 25-hydroxyvitamin D | 2011-04-28 |
20110097734 | Flow Control Technique for Assay Devices - A method for controlling fluid flow in an assay device that employs a membrane is provided. Specifically, one or more recessed regions are formed in the membrane by applying a solvent treatment thereto. The solvent treatment is selected based on its particular dissolving capacity for the material used to form the membrane. For example, an alcohol-based solvent, such as methanol, may be used as a solvent for nitrocellulose membranes. Upon contact with the solvent treatment, a recessed region is formed that may serve a variety of different functions relating to flow control. In one particular embodiment, the recessed region may function as a metering channel that is capable of delivering a controlled volume of the test sample to a detection zone upon initiation of the assay. | 2011-04-28 |
20110097735 | FLUORESCENT PYRENE COMPOUNDS - The present invention relates to fluorescent pyrene dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions. | 2011-04-28 |
20110097736 | METHODS OF DETERMINING LEVELS OF FREE AMINO ACID AND DIPEPTIDES AND DIAGNOSING ALZHEIMER'S DISEASES - Provided herein are methods of diagnosing Alzheimer's disease (“AD”) based on characteristic changes of the levels of certain free amino acids or dipeptides (collectively termed as “AD diagnosis markers”) in the body fluid sample of an individual, carnosine synthesis activities in the plasma, and dopamine synthesis activities in the plasma. Also provided are methods of simultaneously determining the levels of at least two free amino acids or dipeptides in the biological fluid sample of an individual. | 2011-04-28 |
20110097737 | Methods and Compositions for Targeting Proteins of Interest to the Host Cell Envelope - Methods and compositions are provided for producing membrane proteins or toxic proteins from recombinant DNA introduced into a prokaryotic host cell by targeting the expressed proteins to the envelope of the host cell. The methods and compositions utilize a protein vehicle fused to a protein of interest. The fusion protein may contain one or more protease cleavage sites to separate the protein of interest from the protein vehicle either in vivo or in vitro. The protein vehicle is characterized by a membrane-tar peptide and a trans-membrane segment separated by a cytoplasmic amino acid sequence that includes a cytoplasmic affinity-binding domain. | 2011-04-28 |
20110097738 | PLASMA BIOMARKER TOOL FOR THE DIAGNOSIS OF LIVER CANCER COMPRISING LIVER CARBOXYLESTERASE 1 AND LIVER CANCER SCREENING METHOD - The present invention relates to a plasma biomarker for diagnosing hepatocellular carcinoma (HCC), in particular to the discovery of a protein in plasma using 2-D fluorescence differential gel electrophoresis (2-D DIGE), immunoprecipitation and Nano-liquid chromatography mass spectrometry (Nano-LC-MS/MS) system that was unknown on the basis of conventional techniques. By demonstrating the presence of liver carboxylesterase 1 (hCE1) in human plasma and confirming that its secretion level is higher in patients with HCC than in healthy volunteers, this invention may be used as a screening method to diagnose HCC at an early stage. | 2011-04-28 |
20110097739 | BLOOD-BRAIN BARRIER EPITOPES AND USES THEREOF - The invention features a method of identifying an agent and generating an antibody that can cross the blood brain barrier, through the use of novel antigen isoforms of transmembrane domain protein 30A (TMEM30A). This is useful in establishing mechanisms of transmigration across the blood-brain barrier. These antigens are enriched in brain endothelium compared to other endothelial cells and may have better selectivity and capacity for brain delivery compared to transferrin and insulin receptors. One antigen is TMEM30A. | 2011-04-28 |
20110097740 | REAL-TIME CONTINUOUS DETECTION DEVICE - Provided is a real-time continuous detection device for detecting an analyte in a sample including: a sample inflow channel; a sample assay site; and a sample outflow channel, wherein the sample assay site includes a reversible capturing recognizing component and a sensor which detects a signal generated from a binding body of the analyte and the reversible capturing recognizing component. According to the real-time continuous detection device, it is possible to measure a change in concentration of the analyte in real time by continuously recycling the reversible capturing recognizing component. The real-time continuous detection device can be used to detect or assay living organism metabolites, a protein, a hormone, a nucleic acid, a cell, a food test material, an environment contaminant, national-defense chemical, biological and radiological test materials, or the like. Accordingly, the real-time continuous detection device can be applied to medical, public health, national defense, environment, food, veterinary, and biotechnology industries. | 2011-04-28 |
20110097741 | PARTIAL T1R2 NUCLEIC ACID SEQUENCE, RECEPTOR PROTEIN AND ITS USE IN SCREENING ASSAYS - A novel sweet receptor protein, corresponding nucleic acid sequence, expression vectors, transfected host cells, and screening methods for modulators including ligands of the sweet taste response employing the aforementioned are provided. | 2011-04-28 |
20110097742 | CONTRAST AGENTS, METHODS FOR PREPARING CONTRAST AGENTS, AND METHODS OF IMAGING - Embodiments of the present disclosure provide for contrast agents, methods of making contrast agents, and methods of using contrast agents, and the like. | 2011-04-28 |
20110097743 | COMPOSITIONS AND METHODS OF DETECTING TIABS - The present invention relates to novel methods and products for assessing the physiological status of a subject. More particularly, the invention relates to methods of assessing the presence, risk or stage of a cancer in a subject by measuring the levels of antibodies against particular aberrant protein domains in a sample from the subject, or the presence or number of immune cells bearing TCR specific for such aberrant protein domains. The invention is also suitable to assess the responsiveness of a subject to a treatment, as well as to screen candidate drugs and design novel therapies. The invention may be used in any mammalian subject, particularly in human subjects. | 2011-04-28 |
20110097744 | Cellular COPD Diagnosis - Method for diagnosing the risk of a human subject to develop chronic obstructive pulmonary disease (COPD) comprising the steps of:—providing a sample from a human subject,—determining the amount of CD4+CD28null T-cells in said sample,—diagnosing the risk to develop COPD when the amount of CD4+CD28null T-cells is reduced compared to the amount of CD4+CD28null T-cells in healthy human subjects. | 2011-04-28 |
20110097745 | Method For Detecting Microbes - The invention provides means and methods for determining the presence of infecting microbes in a liquid. The method comprises steps of introducing the liquid to the sample container of a microbial analyzer, the sample container further containing nutrients. Other steps include carrying out a first incubation of said specimen, adding bioluminescent tester microbes to the specimen, carrying out a second incubation of the specimen and measuring bioluminescence, thereby determining said presence and/or concentration of infecting microbes. | 2011-04-28 |
20110097746 | Orthogonal Method for the Removal of Transmissible Spongiform Encephalopathy Agents from Biological Fluids - A method comprising contacting a biological fluid comprising hemoglobin and at least one pathogenic agent with a first filter and generating a first filtrate; contacting the first filtrate with a nanofiltration device and generating a second filtrate; contacting the second filtrate with a chromatographic material and isolating an eluted fraction; contacting the eluted fraction with a hydrophobic solvent and generating a hydrophobic and a hydrophilic phase; and isolating the hydrophilic phase wherein the biological fluids comprise components of interest of equal to or less than about 65 kDa. A method comprising contacting a biological fluid comprising high molecular weight components and at least one pathogenic agent with a first filter and generating a first filtrate; contacting the first filtrate with a hydrophilic membrane and generating a second filtrate; contacting the second filtrate with a chromatographic material and isolating an eluted fraction; contacting the eluted fraction with a hydrophobic solvent and generating a hydrophobic and a hydrophilic phase; and isolating the hydrophilic phase, wherein the high molecular weight components have molecular weights greater than about 65 kDa. A method comprising subjecting a biological fluid comprising hemoglobin and at least one pathogenic agent to at least two filtration steps and thereby reducing the amount of pathogenic agent associated with the biological fluid. A method comprising removing transmissible spongiform encephalopathy agents in a hemoglobin solution of human and/or animal origin by subjecting the hemoglobin solution to an orthogonal separation methodology comprising a plurality of filtration steps. | 2011-04-28 |
20110097747 | Monoclonal antibody binds antigen associated with human tumors - The present invention is concerned with a novel antigen associated with human tumors, including carcinomas of the colon, pancreas, and lung, as well as novel monoclonal antibodies which binds strongly to said antigen. The antibodies bind to normal human cells to a much lesser degree than to tumor cells. The antibodies find use both in diagnostic methods such as the detection of malignant cells associated with tumors and in therapeutic methods for treatment of humans with tumors. The novel antigen disclosed is a 60,000 Dalton glycoprotein found on the cell surface of human tumor cells. | 2011-04-28 |
20110097748 | USE OF CELL LINES TO DETERMINE LEVELS OF EFFICACY OF PHARMACEUTICAL FORMULATIONS - The present invention provides methods and assays for determining the efficacy of pharmaceutical compositions. Through the use of cell lines to measure efficacy of a formulation, one can better measure characteristics such as half life, release rate and release profile of a product that may be informative to the FDA. These methods and assays may, for example, be of use when measuring the effect of anti-inflammatory agents on cytokine production that is being considered for local administration. | 2011-04-28 |
20110097749 | PROCESS FOR PRODUCING HEPCIDIN - The present invention provides a novel method for producing biologically functional hepcidin in | 2011-04-28 |
20110097750 | METHOD OF QUANTITATIVE ASSESSMENT ON REPRODUCTIVE EFFORT OF BLACK-LIP PEARL OYSTERS USING ANTIBODY SPECIFIC THERETO - Disclosed are an antigen protein of black-lip pearl oyster egg extract, an antibody specific thereto, and a method of quantitative assessment of the reproductive effort of black-lip pearl oyster using the antibody. The method accomplishes quantitative assessment of reproductive effort of black-lip pearl oysters easily and accurately. In addition, the method provides important information for the study of life cycles of black-lip pearl oysters, and allows efficient management of black-lip pearl oysters. | 2011-04-28 |
20110097751 | GLP-1 RECEPTOR AGONIST BIOASSAYS - Provided herein are cell-based bioassays for measuring GLP-I receptor agonist activity of test compounds, such as GLP-I receptor agonist compounds. Exemplary GLP-I receptor agonist compounds include exendins, exendin analogs, GLP-1(7-37), and GLP-1(7-37) analogs. The bioassays are useful for quantitatively determining cAMP generated in samples containing GLP-I receptor agonist compounds (e.g., exenatide) and 6-23 (clone 6) cells having GLP-I receptors, whereby the amount of cAMP generated can be correlated to the GLP-I receptor agonist activity of the GLP-I receptor agonist compounds (e.g., exenatide). Suitable cell-based bioassays include enzyme-linked immunosorbent assays and homogeneous time-resolved fluorescence assays. | 2011-04-28 |
20110097752 | COPD Diagnosis - The present invention relates to a method for diagnosing chronic obstructive pulmonary disease (COPD) in a human subject or the risk of a human subject to develop COPD comprising the steps of: —providing a sample from a human subject, —determining the amount of caspase-cleaved cytokeratin-18 (ccCK-18), histones, heat shock protein 27 (HSP27), heat shock protein 70 (HSP70) and/or heat shock protein 90 alpha (HSP90 alpha) in said sample, —diagnosing COPD when the amount of ccCK-18, histones, HSP27, HSP70 and/or HSP90 alpha is increased compared to the amount of ccCK-18, histones, HSP27, HSP70 and/or HSP90 alpha in healthy human subjects, or—diagnosing the risk to develop COPD when the amount of ccCK-18, histones and/or HSP70 is reduced compared to the amount of ccCK-18, histones and/or HSP70 in healthy human subjects. | 2011-04-28 |
20110097753 | Chemiluminescent methods and reagents for analyte detection - The present invention relates to chemiluminescent method and regent to detect analyte. One aspect of the current invention relates to using enzyme substrate that can be cleaved by target enzyme to release chemiluminescent compound giving light signal for the detection of varieties of target enzymes. Another aspect of the current invention relates to use chemiluminescent enzyme coupled with analyte binding molecules to detect specific analyte molecules in a homogenous phase. | 2011-04-28 |
20110097754 | METHOD FOR MEASURING ACTIVATED FACTOR VII LEVEL IN A SAMPLE - The present disclosure relates to a method for measuring the activated factor VII level in a sample to be tested, including the steps of: a) mixing the test sample with a plasma free of factor VII (FVII) and free of at least another factor selected from among factor VIII (FVIII), factor IX (FIX), and factor XI (FXI), the test sample+plasma having a final FVII+FVIIa concentration of 10 pM to 80 pM; b) adding initiating components from the thrombin generation reaction; c) obtaining a thrombogram when carrying out a thrombin generation test (TGT) on the mixture from step b); d) comparing at least one of the thrombogram parameters from step c) with a homologous parameter obtained from standard thrombograms established on the basis of standard samples, the activated factor VII level of which is known and varies with each standard sample; e) deducing, from step d), an activated factor VII level measurement in the test sample. | 2011-04-28 |
20110097755 | Apparatus and Method for Optically Sensing Analyte Concentration in an Erythrocyte-Containing Fluid - A device for determination of analytes has optical material with a deposit on a surface thereof. The deposit includes an analyte-reactive reagent and a particulate material for enhancing reflectance of a light beam emitted through the optical material into the analyte-reactive reagent. The enhanced reflectance provides a return of light through the optical material at a level which is not only analytically useful, but involves sufficient returned light intensity so as to enable markedly improved sensitivity and accuracy in analytical determinations. A suitable particulate material is one of hollow spherical particles. Moreover, the particulate material may be selected for a property such as a neutralizing charge that suppresses the membrane-disruptive effects of certain types of optical materials and hemolytic reagents on blood cells such as erythrocytes. | 2011-04-28 |
20110097756 | APEX AS A MARKER FOR LUNG CANCER - The present invention relates to the assessment of lung cancer. It discloses the use of protein AP endonuclease (APEX) in the assessment of lung cancer. It also relates to a method for assessing lung cancer in vitro using a liquid sample derived from an individual by measuring APEX in the sample. Measurement of APEX can, e.g., be used in the early detection or in the follow-up of patients with lung cancer. | 2011-04-28 |
20110097757 | Biomarkers for Diabetes, Obesity, and/or Hypertension - Methods of identifying subjects having, or at risk of developing, diabetes, obesity, and/or hypertension are disclosed, as well as methods of identifying biomarkers for diabetes, obesity, and/or hypertension, and biomarkers identified by such methods. | 2011-04-28 |
20110097758 | Peptide Conjugates and Fluorescence Detection Methods for Intracellular Caspase Assay - Polypeptides labelled with a donor and acceptor pair of dyes selected from a dibenzorhodamine dye and a diamino-benzophenoxazine dye are peptide conjugates which are useful for intracellular and bead-based assays with fluorescence detection. Peptide conjugates with a caspase-recognition site undergo cleavage into peptide fragments which may be detected, located, and quantitated by the changes in fluorescence. Intracellular cleavage of peptide conjugates is correlated with apoptosis. | 2011-04-28 |
20110097759 | Gene Expression Markers for Colorectal Cancer Prognosis - A method of predicting clinical outcome in a subject diagnosed with colorectal cancer comprising determining evidence of the expression of one or more predictive RNA transcripts or their expression products in a biological sample of cancer cells obtained from the subject. | 2011-04-28 |
20110097760 | Method For Producing a Casein Hydrolysate - The present invention relates to a method for producing a casein hydrolysate using a microbial endopeptidase. | 2011-04-28 |
20110097761 | MUTANT MUSCLE-SPECIFIC ENHANCERS - The present invention relates to nucleic acid compositions and expression systems comprising muscle-specific regulatory elements, and methods for expressing heterologous DNA sequences in cells. In particular, the present invention provides mutant muscle-specific enhancers, genetic cassettes, and vectors useful in gene therapy, diagnostic assays, and other gene expression systems. | 2011-04-28 |
20110097762 | LINKERS AND CO-COUPLING AGENTS FOR OPTIMIZATION OF OLIGONUCLEOTIDE SYNTHESIS AND PURIFICATION ON SOLID SUPPORTS - A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications. | 2011-04-28 |
20110097763 | Thermal Cycling Method - The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target. | 2011-04-28 |
20110097764 | AMPLIFICATION PRIMERS WITH NON-STANDARD BASES FOR INCREASED REACTION SPECIFICITY - Described herein are methods for increasing the annealing specificity of an amplification reaction using Iso-base Amplification Primers (“IAPs”). IAPs containing an iso-region are capable of regulating sequence-specific annealing thereby enhancing primer-template hybridization for sequence-specific amplification of nucleotides. | 2011-04-28 |
20110097765 | ENHANCED FERMENTATION PROCESS USING MOLASSES - Described herein are compositions and methods for enhanced fermentation of molasses using transglucosidase. | 2011-04-28 |
20110097766 | 1,2 - ADDITION OF CARBONYL COMPOUNDS USING THE ENZYME YERE - A method is disclosed for forming a covalent bond between two groups having carbonyl radicals in accordance with the present reaction equation: | 2011-04-28 |
20110097767 | MICROORGANISMS FOR THE PRODUCTION OF ANILINE - A non-naturally occurring microbial organism having an aniline pathway includes at least one exogenous nucleic acid encoding an aniline pathway enzyme expressed in a sufficient amount to produce aniline. The aniline pathway includes (1) an aminodeoxychorismate synthase, an aminodeoxychorismate lyase, and a 4-aminobenzoate carboxylyase or (2) an anthranilate synthase and an anthranilate decarboxylase. A method for producing aniline, includes culturing these non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce aniline. | 2011-04-28 |
20110097768 | Novel Burkholderia Multivorans LG 31-3, Amidase Produced From the Same, and Method for Resolution of Racemic Mixture Using the Same - The present invention relates to a novel | 2011-04-28 |
20110097769 | MODIFIED MICROORGANISM USES THEREFOR - The invention provides a genetically modified microorganism that acquires the ability to consume a renewable feedstock (such as cellulose) and produce products. This organism can be used to ferment cellulose, one of the most abundant renewable resources available, and produce products. | 2011-04-28 |
20110097770 | Novel method to generate bioactive compounds in algae - The present invention discloses a novel microalgae-based method, called Microalgae Genomics Technology™ (MaGT), which can be used to generate novel compounds or increase the existing compounds in algae, and revealed some example of its potential applications. Specifically, this method utilizes microalgae genomics technology to manipulate the metabolic pathways in the genomes of microalgae and therefore induce the algae to produce novel secondary compounds or increase the concentration of existing compounds with a variety of commercial applications. | 2011-04-28 |
20110097771 | ISOLATED POLYNUCLEOTIDES AND POLYPEPTIDES AND METHODS OF USING SAME FOR INCREASING PLANT UTILITY - Provided are isolated polypeptides comprising the amino acid sequence at least 80% homologous to SEQ ID NO:68, 51-66, 69-100, 379-656, 707-715, 720-723, 742-754, 764-771 or 772 with the proviso that the amino acid sequence is not as set forth by SEQ ID NO: 765 or 771, isolated polynucleotides comprising the nucleic acid sequence at least 80% identical to SEQ ID NOs:18, 1-16, 19-50, 101-378, 657-672, 674-706, 716-719, 724-741 and 755-763 with the proviso that the nucleic acid sequence is not as set forth by SEQ ID NO:756 or 762, and isolated polynucleotides selected from the group consisting of SEQ ID NOs:779-792 and methods of using same for increasing oil content, yield, growth rate, biomass, vigor, abiotic stress tolerance and/or nitrogen use efficiency of a plant. | 2011-04-28 |
20110097772 | METHOD FOR PRODUCING XYLITOL FROM LIGNOCELLULOSIC HYDROLYSATES WITHOUT DETOXIFICATION - A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeast | 2011-04-28 |
20110097773 | METHOD FOR PRODUCING BUTANOL USING EXTRACTIVE FERMENTATION - A method for producing butanol through microbial fermentation, in which the butanol product is removed by extraction into a water-immiscible extractant composition comprising a first solvent and a second solvent, is provided. The first solvent is selected from the group consisting of C | 2011-04-28 |
20110097774 | VISIBLE LIGHT-ENHANCED ENZYMATIC PROMOTION OF HYDROCARBON REACTIONS - A photocatalytic method for the hydroxylation of alkanes involving the use of a Cytochrome P450 class of enzyme as a catalyst which is activated by exposure to a pulsed blue light emitted from a light emitting diode (LED) at 450 nm±25 nm. | 2011-04-28 |
20110097775 | PRODUCTION OF BUTANOL - The present invention relates to methods for making recombinant thermophilic bacteria of the family Bacillaceae. In particular, recombinant thermophilic Bacillaceae are provided which have been engineered to produce butanol and/or butyrate. Preferably, heterologous nucleic acid molecules encoding one or more butanol or butyrate biosynthetic pathway enzymes are introduced into a thermophilic Bacillaceae in order to produce a recombinant thermophilic Bacillaceae which is capable of producing butanol. The Bacillaceae is preferably of the genus | 2011-04-28 |
20110097776 | NOVEL ETHANOL-PRODUCING YEAST - A novel isolated | 2011-04-28 |
20110097777 | Processes for Extraction of Sugar From Sugar-Bearing Plant Material - The invention provides processes for the extraction of sugar from sugar-bearing plant material such as sugar cane. Accordingly, in one aspect of the invention, a process for extracting sugar from sugar-bearing plant material comprises contacting the sugar-bearing plant material with an extractant solution comprising water and a surfactant; and separating the extractant solution from the sugar-bearing plant material. One example of a suitable surfactant is a poly(alkylene oxide) polymer such as a poly(propylene oxide) polymer, a poly(ethylene oxide) polymer, or a poly(propylene oxide)/(ethylene oxide) copolymer. | 2011-04-28 |
20110097778 | CHIMERIC ALPHA-AMYLASE VARIANTS - Chimeric alpha amylases having the characteristics of high thermostability and good performance in starch degradation, especially high-temperature liquefaction processes, are provided. The alpha-amylases are chimeras of AmyL and AmyS enzymes, and are useful in starch degradation processes. Methods of making the chimeric enzymes, and methods of using the chimeric alpha-amylases for liquefaction, cleaning starch residue from a surface, and treating woven material to remove coatings. Kits for practicing the methods are provided. Polynucleotides encoding the chimeric amylases, vectors, and expression hosts also are provided. | 2011-04-28 |
20110097779 | Processes for Producing Fermentation Products - The present invention relates to processes for producing a fermentation product from gelatinized and/or un-gelatinized starch-containing material using a metallo protease, and processes for producing a fermentation product from gelatinized starch-containing material using a metallo protease and a pullulanase. | 2011-04-28 |
20110097780 | Method of Xylose Fermentation Having Enhanced Conversion Rate in Fiber Solution - A method is provided for improving the efficiency of xylose fermentation in lignocellulosic hydrolysate. The disclosed embodiment raises the efficiency of xylose conversion by adding a specific lignocellulosic material during fermentation. In particular, a 10% enhancement in the efficiency of xylose conversion for ethanol production was given, and the ethanol yield is achieved 90% after adding the specific lignocellulosic material. | 2011-04-28 |
20110097781 | IMMOBILIZED BIOLOGICAL MATERIAL WITH IMPROVED FUNCTIONALITY AND METHOD FOR PRODUCING THE SAME - The present invention provides methods and systems for performing biological reactions with biologically active entities immobilized on a solid support. Particularly, the invention provides a biologically active entity immobilized a support through a spacer linked to a first linker. The spacer gives the biologically active entity a certain level of free movement relatively to the support surface to which it is fixed, thereby allowing the biological reaction to take place. | 2011-04-28 |
20110097782 | METHOD, AGENT, AND KIT FOR ISOLATING NUCLEIC ACIDS - A method for isolating nucleic acids is provided. The method includes providing a biological sample containing at least one nucleic acid, and mixing the biological sample with an isolating agent under a suitable condition to isolate the nucleic acids from the biological sample in single step, wherein the isolating agent contains 1-40 wt % of PEG and/or more than 30 wt % of low molecular weight alcohol, a salt, and a detergent. Isolated nucleic acids are bound to a solid support by changes in the solubility of nucleic acids. Additionally, the present invention further provides an isolating agent and kit for isolating nucleic acids. | 2011-04-28 |
20110097783 | Proteases from gram-positive organisms - The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for | 2011-04-28 |
20110097784 | THERMOSTABLE ORGANIC SOLVENT TOLERANT PROTEASE FROM GRAM-POSITIVE BACTERIA - The present invention relates to a thermostable protease useful as an enzyme for industrial use, a gene encoding the same and a method of producing the enzyme by genetic engineering technique. More particularly, the present invention discloses a thermostable organic solvent tolerant protease and its code gene and application. The invention also discloses a method for preparing protease by isolating from | 2011-04-28 |
20110097785 | METHOD OF REPLICATING VIRUSES IN SUSPENSION CULTURES OF DOG KIDNEY CELLS - Animal cells are described which can be infected by viruses and which are adapted to growth in suspension in medium free of animal-derived components, such as serum-free medium. Processes for the replication of viruses in cell culture using these cells are furthermore described, as well as vaccines which contain the viruses or antigenic portions thereof obtainable by the process. | 2011-04-28 |
20110097786 | Methods of saccharification of polysaccharides in plants - Saccharification of polysaccharides of plants is provided, where release of fermentable sugars from cellulose is obtained by adding plant tissue composition. Production of glucose is obtained without the need to add additional β-glucosidase. Adding plant tissue composition to a process using a cellulose degrading composition to degrade cellulose results in an increase in the production of fermentable sugars compared to a process in which plant tissue composition is not added. Using plant tissue composition in a process using a cellulose degrading enzyme composition to degrade cellulose results in decrease in the amount of cellulose degrading enzyme composition or exogenously applied cellulase required to produce fermentable sugars. | 2011-04-28 |
20110097787 | CONSTANT-TEMPERATURE EQUIPMENT - Constant-temperature equipment wherein maintenance is facilitated with the least failure, and highly reliable culturing and testing can be carried out. Mechanical and electrical structures are eliminated from the inside of a temperature-controlled chamber ( | 2011-04-28 |
20110097788 | NANO AND MICRO-TECHNOLOGY VIRUS DETECTION METHOD AND DEVICE - The invention relates to methods and devices for detecting the presence of a particle of interest (hereinafter an analyte particle) in a fluid. A detection device exemplary of the present invention filters a sample of the fluid to remove particles larger than the analyte particles. A reagent solution, containing reagent particles smaller than the analyte particles, is then added to the sample. The reagent particles will react with the analyte particles, if any are present, to form reagent-analyte complexes which are larger than the analyte particles. The sample is then filtered a second time to remove particles the same size as or smaller than the analyte particles. The sample is then tested for the presence of reagent-analyte complexes to detect the presence of the analyte particle in the fluid. | 2011-04-28 |
20110097789 | TUBE PORTS AND RELATED CONTAINER SYSTEMS - A tube port includes a tubular first stem having an interior surface and an exterior surface extending between a first end and a longitudinally spaced apart second end. The interior surface bounds a passage that longitudinally extends through the first stem. The tube port also includes a flange encircling and radially outwardly projecting from the first end of the first stem. The flange and the first stem are integrally formed as a unitary member comprised of a flexible elastomeric material having a durometer on a Shore A scale with a value of less than 90. | 2011-04-28 |
20110097790 | Lockable cell growth chamber with aintilocking feature - A lockable cell growth chamber is disclosed wherein the lid and base of the dish are provided with at least two pairs of locking and anti-locking members, the locking and antilocking functions being activated by application of rotational torque. | 2011-04-28 |
20110097791 | Novel process, construct and conjugate for producing multiple nucleic acid copies - This invention provides inter alia an in vitro process for producing multiple specific nucleic acid copies in which the copies are produced under isostatic conditions, e.g., temperature, buffer and ionic strength, and independently of any requirement for introducing an intermediate structure for producing the copies. In other aspects, the invention provides in vitro processes for producing multiple specific nucleic acid copies in which the products are substantially free of any primer-coded sequences, such sequences having been substantially or all removed from the product to regenerate a primer binding site, thereby allowing new priming events to occur and multiple nucleic acid copies to be produced. This invention further provides a promoter-independent non-naturally occurring nucleic acid construct that produces a nucleic acid copy or copies without using or relying on any gene product that may be coded by the nucleic acid construct. Another aspect of this invention concerns a protein-nucleic acid construct in the form of a conjugate linked variously, e.g., covalent linkage, complementary nucleic acid base-pairing, nucleic acid binding proteins, or ligand receptor binding. Further disclosed in this invention is an in vivo process for producing a specific nucleic acid in which such a protein-nucleic acid construct conjugate is introduced into a cell. A still further aspect of the invention relates to a construct comprising a host promoter, second promoter and DNA sequence uniquely located on the construct. The host transcribes a sequence in the construct coding for a different RNA polymerase which after translation is capable of recognizing its cognate promoter and transcribing from a DNA sequence of interest in the construct with the cognate promoter oriented such that it does not promote transcription from the construct of the different RNA polymerase. | 2011-04-28 |
20110097792 | INTERLEUKIN-12P40 VARIANTS WITH IMPROVED STABILITY - Modified interleukin-12 (IL-12) p40 polypeptides are disclosed. The modified polypeptides have alterations in the IL-12p40 subunit to eliminate the protease site between positions Lys260 and Arg261. The modified IL-12p40 polypeptides according to the invention have improved stability compared to wild-type mature human IL-12p40 polypeptides. | 2011-04-28 |
20110097793 | CELL SEPARATION DEVICE, CELL SEPARATION SYSTEM AND CELL SEPARATION METHOD - A cell separation device which can perform a continuous processing without bonding fluorescent molecules or magnetic particles to the surface of the cell membrane, a cell separation system, and a cell separation method, wherein when a sample cell suspension containing the desired target cells is supplied continuously from a sample inlet and physiological saline is supplied continuously from a physiological saline inlet, the sample cell suspension flows together with the physiological saline in a liquid flow path and an adsorption force acts on the target cells due to affinity bonding from the adsorbing portions of adsorbing regions in the form of strips formed in a planar wall portion. Since the adsorbing regions in the form of strips are disposed in an asymmetric fashion to the flow path direction of the liquid flow path, the adsorption force acting on the target cells has a constituent perpendicular to the flow path direction. As a result, the target cells shown in | 2011-04-28 |
20110097794 | Whitefly Ecdysone Receptor Nucleic Acids, Polypeptides, and Uses Thereof - The present invention relates to a novel isolated whitefly ecdysone receptor polypeptide. The invention also relates to an isolated nucleic acid encoding the whitefly ecdysone receptor polypeptide, to vectors comprising them and to their uses, in particular in methods for modulating gene expression in an ecdysone receptor-based gene expression modulation system and methods for identifying molecules that modulate whitefly ecdysone receptor activity. | 2011-04-28 |
20110097795 | Desiccated Biologics And Methods Of Preparing The Same - The present invention provides compositions comprising desiccated biologics comprising a cell, protein, virus, nucleic acid, carbohydrate, or lipid, or any combination thereof, along with at least one membrane penetrable sugar, and at least one membrane impenetrable sugar, wherein the moisture content is from 5% to 95%, and to methods of preparing the same, and to methods of treating animals using the same. | 2011-04-28 |
20110097796 | METHOD FOR FREEZING AND STORING CELLS - This invention relates to a method for freezing and storing cells in a container, and relates to frozen cells in freezing medium in a container. | 2011-04-28 |
20110097797 | Modular Functional Peptides for the Intracellular Delivery of Nanoparticles - Described are peptides for delivery of a nanoparticle to the cytosol, the peptide comprising: (a) a nanoparticle association domain; (b) a proline-rich spacer domain; (c) an uptake domain; and (d) a vesicle escape domain comprising a non-hydrolyzable lipid moiety, wherein the spacer domain is between the nanoparticle association domain and the uptake and vesicle escape domains, and wherein the peptide, when attached to an extracellular nanoparticle, is effective to induce uptake of the nanoparticle by a cell and delivery of the nanoparticle to the cytosol of the cell. Also described are methods of delivery of a nanoparticle to the cytosol of a cell, the method comprising providing to a cell a nanoparticle attached to such a peptide. Exemplary nanoparticles include quantum dots. | 2011-04-28 |
20110097798 | MAMMALIAN CELL EXPRESSION VECTORS AND UTILIZATION - An improved mammalian expression vector system which allows: (1) highly expressing exogenous proteins in host mammalian cells; (2) rapidly and efficiently screening recombinant stable cell lines expressing the gene of interest; (3) maintain sustainable expression and prevent gene silencing; and (4) effectively secreting proteins into media in some of cases. The entire expression vector system includes optimized promoters and core promoters, the use of special internal ribosome entry sites, integration of the bacterial backbone into the mammalian expression unit, multiple choices of selection markers, artificial matrix attachment region elements, effective secreting lead sequences and their 5′ and 3′ UTRs, and proper combinations of these expression elements. | 2011-04-28 |
20110097799 | CARDIOMYOCYTE PRODUCTION - Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided. | 2011-04-28 |
20110097800 | METHOD AND APPARATUS FOR RETAINING AND RECIRCULATING CELLS - The invention relates to a device and a method for retaining and recirculating cells in a vessel through which flow passes continuously or batchwise. | 2011-04-28 |
20110097801 | TISSUE SCAFFOLDING COMPOSITES - A method for preparing a biocompatible polymeric composite includes modifying a first biocompatible polymer with a primer group to form a modified biocompatible polymer; blending the modified biocompatible polymer with a second biocompatible polymer and an inorganic material; allowing the primer group of the modified biocompatible polymer to react with the inorganic material to form a biocompatible polymeric composite. Such biocompatible polymeric composites may be formed into medical devices such as tissue growth scaffolds and bone growth scaffolds. | 2011-04-28 |
20110097802 | ORGANIC-INORGANIC COMPOSITE DISPERSION, CELL CULTURE SUBSTRATE MANUFACTURED USING THE SAME, AND METHODS FOR PREPARING THE SAME - Disclosed are: an organic-inorganic complex dispersion improved in film formability and adhesion to a base material. The organic-inorganic complex dispersion comprises an aqueous medium and particles of a complex dispersed in the aqueous medium, wherein the complex has a three-dimensional network structure formed by a polymer of a monomer comprising a monomer represented by general formula (1) and at least one inorganic material selected from a water-swellable clay mineral and silica. Also disclosed is an antifogging material manufactured by using the organic-inorganic complex dispersion. Further disclosed is a cell culture substratum improved in the detachability of cells cultured on the substratum, which is manufactured by using the organic-inorganic complex dispersion. Still further disclosed are manufacturing methods for same. [In the formula, R1 represents a hydrogen atom or a methyl group; R2 represents an alkylene group having 2 to 3 carbon atoms; R3 represents a hydrogen atom or an alkyl group having 1 to 2 carbon atoms; and n represents a number of 1 to 9.] | 2011-04-28 |
20110097803 | CLDN5 Mini-Promoters - Isolated polynucleotides comprising a CLDN5 mini-promoter are provided. The mini-promoter may be operably linked to an expressible sequence, e.g. reporter genes, genes encoding a polypeptide of interest, regulatory RNA sequences such as miRNA, siRNA, anti-sense RNA, etc., and the like. In some embodiments a cell comprising a stable integrant of an expression vector is provided, which may be integrated in the genome of the cell. The mini-promoter may also be provided in a vector, for example in combination with an expressible sequence. The polynucleotides find use in a method of expressing a sequence of interest, e.g. for identifying or labeling cells, monitoring or tracking the expression of cells, etc. | 2011-04-28 |