06th week of 2014 patent applcation highlights part 49 |
Patent application number | Title | Published |
20140038197 | SYSTEM FOR AND METHOD OF DETERMINING CANCER PROGNOSIS AND PREDICTING RESPONSE TO THERAPY - A database for predicting clinical outcomes based upon quantitative tumor burden in lymph node samples from an individual is provided. The database comprises data sets from a plurality of individuals. The data sets include clinical outcome data and data regarding number of lymph nodes evaluated, maximum number of biomarker detected in any single node, median normalized expression levels detected across all evaluated lymph nodes and the maximum normalized expression levels detected in any evaluated lymph nodes and the database also includes stratified risk categories based upon recursive partitioning of data. A system for predicting clinical outcomes based upon quantitative tumor burden in lymph node samples from an individual is provided which includes the database linked to a data processor, an input interface and an output interface. Method of preparing a database and method for predicting clinical outcome for a test patient based upon quantitative tumor burden in lymph node samples from an individual using a system that includes the database linked to a data processor, an input interface and an output interface. The method comprises measuring quantitative tumor burden in a plurality of lymph node samples from an individual, inputting the results into the system and processing with data in the database. The results of the processing of the data is the assignment of data test patient to a stratified risk category. Output is produced that displays test patient's identity and assigned stratified risk category. | 2014-02-06 |
20140038198 | CANNABINOID 2 (CB2) RECEPTOR GENE PROMOTER AND UNIQUE RNA TRANSCRIPTS IN B CELLS AND METHODS OF USE - Cannabinoid receptor 2 (CB | 2014-02-06 |
20140038199 | LUMINESCENCE DETECTION METHOD - A method of detecting an analyte is provided. The method includes providing a sample, a container | 2014-02-06 |
20140038200 | METHODS AND COMPOSITIONS FOR AMPLIFYING A DETECTABLE SIGNAL - Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex. | 2014-02-06 |
20140038201 | SUB-DIFFRACTION LIMIT IMAGE RESOLUTION IN THREE DIMENSIONS - The present invention generally relates to sub-diffraction limit image resolution and other imaging techniques, including imaging in three dimensions. In one aspect, the invention is directed to determining and/or imaging light from two or more entities separated by a distance less than the diffraction limit of the incident light. In some cases, the position of the entities can be determined in all three spatial dimensions (i.e., in the x, y, and z directions), and in certain cases, the positions in all three dimensions can be determined to an accuracy of less than about 1000 nm. In some cases, the z positions may be determined using one of a variety of techniques that uses intensity information or focal information (e.g., a lack of focus) to determine the z position. Non-limiting examples of such techniques include astigmatism imaging, off-focus imaging, or multi-focal-plane imaging. | 2014-02-06 |
20140038202 | ASSAY DETECTION SYSTEMS AND METHODS - Described herein are methods and devices for detecting one or more targets in a sample using an assay device, optical illumination, and optical detection. | 2014-02-06 |
20140038203 | METHODS FOR DETECTING OR PREDICTING KIDNEY DISEASE - Methods of detecting or predicting the onset or magnitude of kidney diseases such as acute kidney disease (AKI), previously called acute renal failure (ARF), are provided. In various aspects, methods and kits are provided to detect specific urinary proteins associated with AKI diagnosis or prognosis such as, e.g., angiotensinogen. | 2014-02-06 |
20140038204 | SELECTIVE MEASUREMENT OF ACTIVE HUMAN PROTEASE COAGULATION FACTORS - The present invention relates to a method for the selective determination of the concentration of an active human protease coagulation factor in a sample, comprising the steps of binding a specific inhibitor of the human protease coagulation factor to a solid phase, letting the human protease coagulation factor contained in the sample bind to the solid phase-bound inhibitor, and detecting the human protease coagulation factor bound to the solid phase-bound inhibitor with a detection reagent. | 2014-02-06 |
20140038205 | METHODS AND COMPOSITIONS RELATING TO COAGULATION ASSAYS - The invention provides methods and compositions relating to the detection and neutralization of heparin and heparin derivatives in vivo and in vitro. To neutralize heparin in a patient sample, a composition comprising a complex of a heparin-binding agent and a carrier compound is used prior to performing coagulation assays. | 2014-02-06 |
20140038206 | IMAGE ANALYSIS AND MEASUREMENT OF BIOLOGICAL SAMPLES - Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples. | 2014-02-06 |
20140038207 | PLATELET ANALYSIS SYSTEM - A method for diagnosis of HIT (Heparin-induced thrombocytopenia) in a patient's serum or plasma sample and a system comprising kits for performing the method. | 2014-02-06 |
20140038208 | PREPARATION OF ANTI-PEG ANTIBODY EXPRESSING CELL AND APPLICATION THEREOF - The present invention provides a method for preparing an isolated eukaryotic cell which presents an anti-polyethylene glycol (PEG) antibody on a cell membrane. The present invention also provides a method for a quantitative analysis of a polyethylene glycol (PEG) by said anti-PEG antibody expressing cell. The cell-based quantitative analysis of the present prevention could sensitively quantify free PEG and PEG-modified macromolecules (proteins, nanoparticles and liposomes) as sensitive as nano-gram level. | 2014-02-06 |
20140038209 | Apparatus and Methodology to Carry Out Biochemical Testing on a Centrifugal Platform Using Flow Splitting Technique - The invention provides an apparatus and methodology to carry out biochemical testing on a centrifugal platform using flow splitting technique. In conventional biochemical testing, reagents need to be loaded individually into each reservoir. By using the flow splitting technique in this invention, one reagent only need to be loaded once, then, it can be evenly distributed into each reaction chambers in single or multiple layers format. The invention greatly reduces the required manpower when large numbers of assays are integrated on one platform. Because of the invention, many medical examinations can be performed efficiently, thus reduce the waste of manpower, time and cost. | 2014-02-06 |
20140038210 | ANTI-HUMAN IgG1 ANTIBODY - Herein are reported a monoclonal antibody specifically binding to a human IgG1 antibody and not specifically binding to the immunoglobulin of an experimental animal and the use of the antibody in immunoassays. | 2014-02-06 |
20140038211 | BREAST CANCER DIAGNOSIS USING NIPPLE DISCHARGE - A panel of biomarkers for the prediction of breast cancer using nipple discharge, as well as method and kits for using the same. Nipple discharge may be nipple aspirate fluid or spontaneous discharge. The panel has been selected to optimize predictive ability while minimizing the number of markers to be screened. As observed, the panel approaches one hundred percent accuracy for predicting breast cancer without the need for invasive biopsy. | 2014-02-06 |
20140038212 | IMMUNOASSAY METHODS - The application generally relates to the field of diagnostic or prognostic assays and in particular relates to assays for the detection of antibodies in a sample comprising patient bodily fluid, wherein such antibodies are used as biological markers of a disease state or disease susceptibility. The assay is based on cross-titration of both the patient bodily fluid to be tested for the antibody and an antigen used to detect the antibody by specific binding. | 2014-02-06 |
20140038213 | HIGH-MOLECULAR-WEIGHT ADIPONECTIN MEASUREMENT METHOD - Provided is a method of separating and measuring highly active HMW adiponectin in adiponectin multimers. A method of measuring high-molecular-weight adiponectin in a sample, wherein adiponectin multimers are separated by use of a protease and measured immunologically, the method comprising reacting a sample containing adiponectin multimers with chymotrypsin. | 2014-02-06 |
20140038214 | MICROFLUIDIC FLOW ASSAY AND METHODS OF USE - A microfluidic-based flow assay and methods of manufacturing the same are provided. Specifically, the microfluidic flow assay includes a substrate surface comprising lipid coated particles and microfluidic channels through which a blood product can flow. The lipid coated particles comprise functional molecules that can induce or inhibit the coagulation cascade. | 2014-02-06 |
20140038215 | DERIVATIZATION OF PNGASE F RELEASED GLYCANS ON AN HPLC CHIP - A microfluidic device for glycan analysis includes a deglycosylation column comprising a glycosidase attached to a solid support; a tagging column comprising a reactive ester for reaction with an amino group, wherein the tagging column is arranged downstream of the deglycosylation column; an analytical column comprising a stationary phase capable of separating a derivatized glycan; and a plurality of inlet/outlet ports configured to connect with channels on a switching element to form flow paths. | 2014-02-06 |
20140038216 | METHOD AND APPARATUS FOR PERFORMING RETENTION TIME MATCHING - A method for matching a precursor ion with one or more related product ions includes providing input data sets obtained from sample injections, each of the data sets including a precursor ion and one or more product ions, normalizing the input data sets in accordance with a single retention time for the precursor ion, determining which product ions are within a predetermined retention time window with respect to the single retention time, and, if a product ion is within the predetermined retention time window for a specified number of the input data sets, determining that the product ion is related to the precursor having the single retention time. An apparatus for analyzing a sample includes a chromatography module, a mass-spectrometry module in communication with the chromatography module, and control unit in communication with the chromatography module and the mass-spectrometry module. | 2014-02-06 |
20140038217 | METHOD AND APPARATUS FOR FRACTIONATION-BASED CHEMICAL ANALYSES | 2014-02-06 |
20140038218 | PROTEOLYSIS DETECTION - Disclosed are electrochemical methods, apparatus, systems and kits for the detection or monitoring of proteolysis of proteinaceous matrices such as fibrin clots. The methods, apparatus, systems and kits generally include the use of voltammetric techniques to measure the changes in current that result from diffusion of an electroactive species towards an electrode on proteolysis of a proteinaceous matrix. | 2014-02-06 |
20140038219 | THYROGLOBULIN QUANTITATION BY MASS SPECTROMETRY - Provided are methods for determining the amount of thyroglobulin in a sample using various purification steps followed by mass spectrometry. The methods generally involve purifying thyroglobulin in a test sample, digesting thyroglobulin to form peptide T129, purifying peptide T129, ionizing peptide T129, detecting the amount of peptide T129 ion generated, and relating the amount of peptide T129 ion to the amount of thyroglobulin originally present in the sample. | 2014-02-06 |
20140038220 | METHOD FOR SCREENING INHIBITORY SUBSTANCES TO INFLAMMATORY SKIN-AGING - Disclosed is a method for screening a substance inhibiting inflammatory skin aging, comprising: treating an immune cell with an inflammatory stimulant; treating a skin cell with a candidate substance; treating the skin cell treated with the candidate substance, with the immune cell treated with the inflammatory stimulant; and measuring the concentration of matrix metalloproteinase-1 (MMP-1) in the skin cell treated with the immune cell. | 2014-02-06 |
20140038221 | BIOENGINEERED HUMAN TRABECULAR MESHWORK FOR BIOLOGICAL APPLICATIONS - The present invention relates to methods of manufacture and utility of an artificial trabecular meshwork [TM] that utilizes micro- and nanofabricated materials bioengineered to mimic the structure and function of native outflow system of the eye. | 2014-02-06 |
20140038222 | Compact portable apparatus for optical assay - A structure is configured to retain optical diagnostic assay components in a manner that enables them to be synergistically combined or coupled both mechanically and electrically with a conventional mobile electronic device (such as a smartphone), such that the pairing maintains a mutually advantageous relationship to provide a compact portable optical assay apparatus in which the optical assay portion has access to the image sensor, battery power, microprocessor, and data capture, analysis, storage, display and transmission capabilities of the smartphone, and the smartphone portion provides the overall apparatus with features of transportability, user interface, and information storage, analysis and retrieval, and transmission of assay results to a separate site, such as a site of records related to the provider of the sample being assayed. Methods of providing such structure and of performing an optical assay of sample material utilizing such structure are described. | 2014-02-06 |
20140038223 | CRK-A NOVEL TARGET FOR CANCER THERAPY - This disclosure identifies a novel apoptotic compensatory proliferation signaling complex (ASCS) involving ExoT's cellular target, Crk1. This disclosure demonstrates that in cells induced to undergo apoptosis, Crk1 forms globular complexes which associate with plasma membrane in the apoptotic cell and are released from the dying cell to its healthy neighbor, inducing it to proliferate. ExoT prevents the ACSC assembly while inducing apoptosis, indicating that apoptotic program cell death and compensatory proliferation are distinct and can be uncoupled from each other. Without being bound to any particular theory, it is believed that Crk1 functions as a scaffold for the ACSC assembly and that its ADP-ribosylation by ExoT interferes with this process while resulting in anoikis apoptotic cell death. | 2014-02-06 |
20140038224 | MICROFLUIDIC ELECTROCHEMICAL DEVICE AND PROCESS FOR CHEMICAL IMAGING AND ELECTROCHEMICAL ANALYSIS AT THE ELECTRODE-LIQUID INTERFACE IN-SITU - A microfluidic electrochemical device and process are detailed that provide chemical imaging and electrochemical analysis under vacuum at the surface of the electrode-sample or electrode-liquid interface in-situ. The electrochemical device allows investigation of various surface layers including diffuse layers at selected depths populated with, e.g., adsorbed molecules in which chemical transformation in electrolyte solutions occurs. | 2014-02-06 |
20140038225 | CELL CULTURE DEVICE - A cell culture device for culturing cells and measuring mechanical forces exerted by one or more cells. The device includes a cell culture chamber having a bottom plate and a main frame with side walls to enclose a volume. The main frame has side walls fabricated as one piece. The cell culture chamber is open on a side opposite the bottom plate. The device further has a force sensor array on top of the bottom plate within the cell culture chamber. The force sensor array has an array of flexible microcolumns configured to provide a site of adhesion for one or more cells, each microcolumn being configured to be deflected from an original position to a deflected position in response to a mechanical force exerted by the one or more cells. | 2014-02-06 |
20140038226 | Device For Spraying A Reagent For Fast Microbiological Analysis - The device for spraying a reagent onto a support ( | 2014-02-06 |
20140038227 | APPARATUS AND METHOD FOR PREPARING CYTOLOGICAL SPECIMENS FOR ANALYSES - The apparatus ( | 2014-02-06 |
20140038228 | REMOVABLE LAYER AND METHOD OF USE - A method of processing a sample for analysis is disclosed. The method includes providing a first sample-transfer article with a sample disposed therein, a container ( | 2014-02-06 |
20140038229 | SELECTION OF IMPROVED MICROBIAL STRAINS FOR PRODUCTION OF SUCCINIC ACID FROM GLYCEROL - Provided are methods for generating a microorganism with improved ability to convert glycerol to succinic acid. These methods comprise combining a microorganism with ability to convert glycerol to succinic acid and a medium containing glycerol to produce a fermentation mixture; allowing fermentation to occur such that succinic acid is produced; and assaying for an indication of glycerol metabolism. These latter two steps may be repeated as necessary. Also provided are microorganisms generated in this manner, particularly bacteria deposited at the NCIMB on 13 | 2014-02-06 |
20140038230 | A SIMPLE AND AFFORDABLE METHOD FOR IMMUNOPHENOTYPING USING A MICROFLUIDIC CHIP SAMPLE PREPARATION WITH IMAGE CYTOMETRY - The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and system disclosed herein largely overcome these limitations. The system includes a simple system for CD4 and CD8 counting in point-of-care HIV staging within resource poor countries. Unlike previous approaches, no sample preparation is required with the sample added directly to a chip containing dried reagents by capillary flow. A large area image cytometer consisting of an LED module is used to excite the fluorochromes PerCP and APC labeled targets and a monochrome CCD camera with a combination of two macro lenses captures images of 40 mm | 2014-02-06 |
20140038231 | ISOLATION AND CHARACTERIZATION OF TUMOR CELLS USING SHEAR STRESS MEASUREMENTS - Methods for isolating viable cancer cells from a sample that comprises a mixture of cancerous cells and normal (non-cancerous) cells are provided. In the methods, a fluid preparation comprising a mixture of cancerous and normal cells is repeatedly exposed to fluid shear stresses, whereby the repeated exposure to the fluid shear stresses preferentially imparts fluid shear stress-resistance to the cancerous cells. | 2014-02-06 |
20140038232 | Enhanced Scheduling Sample Processing System and Methods of Biological Slide Processing - A sample processing system | 2014-02-06 |
20140038233 | EXPRESSION VECTOR FOR ANIMAL CELLS - There is provided an expression vector for animal cell having an increased gene expression efficiency, and particularly, an expression vector for animal cells including a MAR element and a SAR element, which are gene expression increasing factors, at a 5′ end of a promoter, a 3′ end of a transcription termination site, or at both of the 5′ end of the promoter and the 3′ end of the transcription termination site. | 2014-02-06 |
20140038234 | Expression System With Sar Element From IFNalpha2 - A short human genomic nucleotide sequence from the SAR3 region of the human interferon α2 gene permits enhances expression stability in the absence of drug selection and permits generation of stable clones or stable pools of cells for producing recombinant proteins. Although stable clones may be generated, the ability to generate stable pools reduces the burden of generating stable clones. | 2014-02-06 |
20140038235 | Methods For Producing Hyaluronan In A Recombinant Host Cell - The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a | 2014-02-06 |
20140038236 | Global Amplification Using a Randomly Primed Composite Primer - The invention relates to the field of polynucleotide amplification. More particularly, the invention provides methods, compositions and kits for amplification of (i.e., making multiple copies of) a multiplicity of different polynucleotide template sequences using a randomly primed RNA/DNA composite primer. | 2014-02-06 |
20140038237 | NUCLEIC ACID MODIFYING ENZYMES - This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid. | 2014-02-06 |
20140038238 | NUCLEIC ACID AMPLIFICATION METHODS - The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein. | 2014-02-06 |
20140038239 | MEGANUCLEASE VARIANTS CLEAVING A DNA TARGET SEQUENCE FROM THE HUMAN HEMOGLOBIN BETA GENE AND USES THEREOF - An I-CreI variant, wherein one of the two I-CreI monomers has at least two substitutions, one in each of the two functional subdomains of the LAGLIDADG core domain situated respectively from positions 26 to 40 and 44 to 77 of I-CreI, said variant being able to cleave a DNA target sequence from the human beta globin gene. Use of said variant and derived products for the prevention and the treatment of pathological conditions caused by a mutation in the human beta globin gene (sickle cell disease, beta-thalassemia). | 2014-02-06 |
20140038240 | METHODS FOR MULTIPART, MODULAR AND SCARLESS ASSEMBLY OF DNA MOLECULES - The present invention consists of methods for joining DNA molecules (parts) together to form larger DNA molecules (assemblies) of specified sequence and organization. The invention exhibits three necessary characteristics. Firstly, the invention enables 2 or more parts to be joined in a single reaction. Secondly, the seam between joined parts is scarless, producing no residual sequence dependencies like restriction enzyme recognition sites. Thirdly, parts are modular and can easily be reused in novel assemblies without modification. Prior technologies have exhibited no more than two of the three necessary characteristics, limiting their utility in synthesizing and editing DNA molecules of arbitrary sequence. | 2014-02-06 |
20140038241 | GENOMIC ENRICHMENT METHOD, COMPOSITION, AND REAGENT KIT - By using engineered sequence specific DNA nuclease (“SSDN”), the composition, reagent kit and method of the present invention can cut and release a DNA sequence of interest 1×10 | 2014-02-06 |
20140038242 | Polypeptides Having Isoamylase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having isoamylase activity derived from | 2014-02-06 |
20140038243 | METHODS FOR PRETREATING BIOMASS - A method for treating biomass is provided, which includes, in a reactor, allowing gaseous ammonia to condense on the biomass and react with water present in the biomass to produce pretreated biomass, wherein reactivity of polysaccharides in the biomass is increased during subsequent biological conversion as compared to polysaccharides in biomass which has not been pretreated. A method for treating biomass with a liquid ammonia and recovering the liquid ammonia is also provided. Related systems which include a biochemical or biofuel production facility are disclosed. | 2014-02-06 |
20140038244 | INTEGRATED WOOD PROCESSING AND SUGAR PRODUCTION - Provided is a system for localizing and optimizing harvesting of woody biomass by providing a plurality of portable pretreatment units at harvesting or mill sites. The sugars derived from woody waste products can be transported to plants for processing or processing directly by plants located at mill sites to generate biofuels and other chemicals. | 2014-02-06 |
20140038245 | EXTRACELLULAR ALDONOLACTONASE - The present disclosure relates to hydrolysis of hexose-δ-lactones by use of an | 2014-02-06 |
20140038246 | METHOD FOR PRODUCING LIGNIN DERIVATIVES - A method for producing lignin derivatives from technical lignins by treatment with proteolytic enzymes (proteases), characterized in that the proteolytic treatment of the technical lignins significantly reduces the molar mass thereof. | 2014-02-06 |
20140038247 | ALGAE HAVING INTRACELLULAR LIPID PARTICLES AND HIGH LIPID CONTENT - A method for increasing the lipid content of algae includes combining algae and a lipid-precipitating addition in a growth-inhibitive medium, the growth-inhibitive medium being deficient in at least one nutrient that is necessary for reproductive growth of algae, thus frustrating algae reproduction, the lipid-precipitating addition selected from hydroxyl-containing compounds and amine-containing compounds. An organic acid addition may also be combined with the lipid-precipitating addition and algae in the growth-inhibitive medium, the organic acid addition including compounds containing carboxylic acid functionality. Direct production of biodiesel is also achieved by the use of particular lipid-precipitating additions and organic acid additions. | 2014-02-06 |
20140038248 | PRODUCT OF FATTY ACID ESTERS FROM BIOMASS POLYMERS - The invention provides consolidated bioprocessing methods and host cells. The host cells are capable of directly converting biomass polymers or sunlight into biodiesel equivalents and other fatty acid derivatives. In particular, the invention provides a method for producing biodiesel equivalents and other fatty acid derivatives from a biomass polymer including providing a genetically engineered host cell, culturing the host cell in a medium containing a carbon source such that recombinant nucleic acids in the cell are expressed, and extracting biodiesel equivalents and other fatty acid derivatives from the culture. | 2014-02-06 |
20140038249 | Integrated Process For The Production Of Jatropha Methyl Ester And By Products - The present invention provides a simpler and more energy efficient process for the preparation of fatty acid methyl ester (biodiesel) from sun dried whole seed capsules of | 2014-02-06 |
20140038250 | APPARATUS AND METHOD FOR SEPARATING AND REFINING PRODUCT MANUFACTURED BY MICROBIAL FERMENTATION BY USING ADSORBENT - The present invention relates to an apparatus and a method for fermenting, separating, and refining a product, which is produced by cultivating a microorganism. The apparatus and the method for fermenting, separating, and refining, of the present invention, can separate and refine the product that is produced by microbial fermentation in a simple, continuous manner and with high efficiency. | 2014-02-06 |
20140038251 | PROCESSING BIOMASS - Fructose, e.g., fructose derived from a cellulosic or lignocellulosic material, is use, e.g., fermented to produce a product, e.g., a solvent. | 2014-02-06 |
20140038252 | PROCESS FOR THE PRODUCTION OF ETHANOL AND BUTANOL - The present invention relates to a process for the production of ethanol and butanol from biomass, and in particular to a process for the production of ethanol and butanol using two separate fermentation step subjecting the biomass feedstock to anaerobic fermentation at a pH below 6.0 and at a temperature in the range 20 to 700° C. and so as to convert the biomass to a product predominantly comprising acetic acid and butyric acid with at least a 2:1 ratio by weight of acetic acid to butyric acid, c) treating the product of stream of step (b) to separate a solution comprising the acetic acid and butyric acid by: (i) separating a solution comprising the acetic acid and butyric acid from any residual solids and (ii) separating bacteria and/or pasteurizing or sterilizing the solution from the first fermentation step, and d) in a second fermentation step fermenting the solution comprising the acetic acid and butyric acid from the step (c) to form ethanol and butanol. | 2014-02-06 |
20140038253 | COMPOSITIONS AND METHODS FOR INCREASED ETHANOL PRODUCTION FROM BIOMASS - The present application discloses the identification of the novel | 2014-02-06 |
20140038254 | Biological/Electrolytic Conversion of Biomass to Hydrocarbons - Hydrocarbon and hydrogen fuels and other products may be produced by a process employing a combination of fermentation and electrochemical stages. In the process, a biomass contained within a fermentation medium is fermented with an inoculum comprising a mixed culture of microorganisms derived the rumen contents of a rumen-containing animal. This inoculated medium is incubated under anaerobic conditions and for a sufficient time to produce volatile fatty acids. The resultant volatile fatty acids are then subjected to electrolysis under conditions effective to convert said volatile fatty acids to hydrocarbons and hydrogen simultaneously. The process can convert a wide range of biomass materials to a wide range of volatile fatty acid chain lengths and can convert these into a wide range of biobased fuels and biobased products. | 2014-02-06 |
20140038255 | Methods and Compositions for Producing Alkenes of Various Chain Length - The NonA alkene synthase in | 2014-02-06 |
20140038256 | PROCESS FOR PREPARING METABOLIZED CONDITIONED GROWTH MEDIA - Compositions comprising metabolized conditioned growth medium and/or metabolized cell extract and methods of use are described. The metabolized conditioned growth medium and metabolized cell extract compositions may be formulated with an acceptable carrier into injectable or topical formulations, for example, as a cream, lotion or gel, and may be used in cosmeceutical or pharmaceutical applications. The metabolized conditioned growth medium and metabolized cell extract may also be further processed to concentrate or reduce one or more factors or components contained within the metabolized conditioned growth medium or metabolized cell extract. The growth medium may be conditioned by any eukaryotic cell. The metabolized conditioned growth medium and metabolized cell extract may be used to prevent or treat a condition, for example, a skin condition. | 2014-02-06 |
20140038257 | METHODS OF USING ULTRASOUND IN TISSUE CULTURE AND TISSUE ENGINEERING - The present disclosure describes methods of using ultrasound in tissue culture and tissue engineering, as well as a bioreactor that includes at least one ultrasound transducer. | 2014-02-06 |
20140038258 | BIOREACTOR WITH MECHANICAL AND ELECTRICAL STIMULATION MEANS - Disclosed herein is a bioreactor for creating and/or conditioning biological tissues, in particular cardiovascular patches. This bioreactor supports additionally cellular differentiation under controlled conditions. The bioreactor comprises a housing having a first section ( | 2014-02-06 |
20140038259 | FLUIDIC IN-LINE PARTICLE IMMOBILIZATION AND COLLECTION SYSTEMS AND METHODS FOR USING THE SAME - Fluidic sorting systems configured to immobilize, and optionally concentrate and/or analyze, one or more particles within the system are provided. Aspects of the systems include a flow-through chamber and an immobilization component configured to immobilize, preferably reversibly, a particle within the flow-through chamber. Optionally, the systems include an analysis component configured to optically analyze an immobilized particle within the flow-through chamber. In certain aspects, the systems are configured to collect one or more particles from the flow-through chamber for subsequent analysis, experimentation, and/or use. Also provided are methods, components and kits for reversibly immobilizing, and optionally analyzing, one or more particles within a fluidic sorting system. | 2014-02-06 |
20140038260 | METHOD FOR PROCESSING A SURFACE - A method for processing a surface involves depositing at least one class of enzymes ( | 2014-02-06 |
20140038261 | Compositions and Methods of Use of Immunotoxins Comprising Ranpirnase (Rap) Show Potent Cytotoxic Activity - The present invention concerns methods and compositions for forming immunotoxin complexes having a high efficacy and low systemic toxicity. In preferred embodiments, the toxin moiety is a ranpirnase (Rap), such as Rap(Q). In more preferred embodiments, the immunotoxin is made using dock-and-lock (DNL) technology. The immunotoxin exhibits improved pharmacokinetics, with a longer serum half-life and significantly greater efficacy compared to toxin alone, antibody alone, unconjugated toxin plus antibody or even other types of toxin-antibody constructs. In a most preferred embodiment the construct comprises an anti-Trop-2 antibody conjugated to Rap, although other combinations of antibodies, antibody fragments and toxins may be used to form the subject immunotoxins. The immunotoxins are of use to treat a variety of diseases, such as cancer, autoimmune disease or immune dysfunction. | 2014-02-06 |
20140038262 | NOVEL PAENIBACILLUS SP. HPL-001 STRAIN FOR PRODUCING HIGHLY ACTIVE XYLANASE HAVING HEAT RESISTANCE AND A WIDE PH RANGE, NOVEL XYLANASE ENZYME ISOLATED THEREFROM, AND METHOD FOR MASS-PRODUCING SAME USING A TRANSFORMANT THEREOF - The present invention relates to the novel | 2014-02-06 |
20140038263 | Activity of Fe-S Cluster Requiring Proteins - The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell. | 2014-02-06 |
20140038264 | METHOD OF PRODUCING A POLYPEPTIDE OR VIRUS OF INTEREST IN A CONTINUOUS CELL CULTURE - Described herein is a chemostat-like continuous cell culture system that combines certain advantages of perfusion open systems and chemostat open systems to improve the culturing of mammalian cells, e.g., genetically modified cells, particularly in serum-free or chemically-defined media. The continuous culture system described herein involves culturing mammalian cells in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system has a dilution rate (D) of less than about 2 d | 2014-02-06 |
20140038265 | COMPOSITE OIL DISPLACEMENT AGENT CONTAINING A MICROORGANISM FOR OIL DISPLACEMENT AND A PREPARATION METHOD THEREOF - The present invention relates to a microorganism for oil displacement, a composite oil displacement agent containing the microorganism and a preparation method thereof. The microorganism for oil displacement contains the following main components: 20 to 40% of microorganism, 6 to 30% of surfactant, 5 to 10% of macromolecular modifier, 1 to 5% of viscosity reducer, 1 to 5% of additive and the balance of water. The preparation method comprises the steps of in proportion by weight, adding the microorganism, the surfactant, the macromolecular modifier, the viscosity reducer, the additive and the water to a reactor provided with a stirring device, and stirring the components for 1.5 to 2 h at room temperature to obtain the finished product. According to the invention, the microorganism and the surfactant composite oil displacement agent are prepared based on the mechanism of intermolecular interaction of the surfactant of a plurality of association and active components with complementary structure and good compatibility, and the system has high temperature and salt resistance, is capable of reducing viscosity of crude oil and oil-water interfacial tension, meets the demand of environmental protection, is suitable for oil recovery and can enhance the recovery rate of crude oil remarkably. | 2014-02-06 |
20140038266 | OIL ABSORBENT COMPOSITION - The invention relates to a method of preparing an oil absorbent composition. The method comprises heating and then de-mineralising a precursor plant material under conditions suitable to produce an oil absorbent composition comprising charcoal. The invention extends to oil absorbent compositions per se, such as charcoal-based compositions, and to various uses of the compositions for efficiently and rapidly absorbing spilled oil, for example from water surfaces, or from bituminous sands. | 2014-02-06 |
20140038267 | NOVEL MONOMERIC YELLOW-GREEN FLUORESCENT PROTEIN FROM CEPHALOCHORDATE - The present disclosure provides isolated nucleic acid sequences encoding a monomeric green/yellow fluorescent proteins, and fragments and derivatives thereof. Also provided is a method for engineering the nucleic acid sequence, a vector comprising the nucleic acid sequence, a host cell comprising the vector, and use of the vector in a method for expressing the nucleic acid sequence. The present invention further provides an isolated nucleic acid, or mimetic or complement thereof, that hybridizes under stringent conditions to the nucleic acid sequence. Additionally, the present invention provides a monomeric green/yellow fluorescent protein encoded by the nucleic acid sequence, as well as derivatives, fragments, and homologues thereof. Also provided is an antibody that specifically binds to the green/yellow fluorescent protein. | 2014-02-06 |
20140038268 | Activity of Fe-S Cluster Requiring Proteins - The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell. | 2014-02-06 |
20140038269 | METHOD FOR PRODUCING DRY MICROBIAL CELL POWDER - An object of the present invention is to develop a means capable of maintaining the enzymatic activity of a microorganism even in a dry state. Provided is a method for producing a dry microbial cell powder maintaining an enzyme titer, characterized by comprising adding a carbohydrate to a liquid of microbial cells having an enzymatic activity and then drying the liquid. Also provided is a dry microbial cell powder obtained by the method. | 2014-02-06 |
20140038270 | Detergent Compositions Comprising Metalloproteases - The present invention relates to the use of Thermolysin-Like Metalloproteases in cleaning processes, such as laundry and dish wash, and in particular to the use in low temperature wash and in removal of egg stains. The invention also relates to detergent compositions and cleaning compositions comprising Thermolysin-Like Metalloproteases. | 2014-02-06 |
20140038271 | METHODS FOR CANCER DETECTION, DIAGNOSIS AND PROGNOSIS - The present invention provides a method for diagnosing cancer, predicting a disease outcome or response to therapy in a patient sample. The method involves isolating a circulating tumor cell (CTC), for example, a viable CTC, from a sample using a parylene microfilter device comprising a membrane filter having or consisting of a parylene substrate, which has an array of holes with a predetermined shape and size; and detecting and quantifying telomerase activity in blood circulating tumor cells. The invention further provides methods of using cells live-captured in various applications. | 2014-02-06 |
20140038272 | THERMAL CYCLING SYSTEM AND METHOD OF USE - A temperature cycling system ( | 2014-02-06 |
20140038273 | Nano Biofuel Production Processes: Using Nantechnology to Enhance Produciton fo Biofuels - Methods, systems, devices and materials for producing biofuels under nanoscale control (“nanobiofuels”) are provided. In one aspect, the invention provides method for producing a biofuel, including providing a hydrocarbon producing organism; exposing the biological hydrocarbon producing organism to conditions effective to cause substantial release of the hydrocarbon from the biological hydrocarbon producing organism; and isolating at least a portion of the hydrocarbon. At least one of the actions of providing, exposing, and isolating is performed using a corresponding nanoscale control. | 2014-02-06 |
20140038274 | NUCLEIC ACID ANALYSIS DEVICE, NUCLEIC ACID ANALYSIS APPARATUS, AND NUCLEIC ACID ANALYSIS METHOD - The present invention relates to a nucleic acid analysis device in a nucleic acid analysis apparatus, whereby waste of reaction spots on the nucleic acid analysis device is eliminated and leakage of fluorescence excitation light to unobserved nucleic acid measurement regions is minimized. Specifically, the nucleic acid analysis device has a plurality of nucleic acid measurement regions, which are characterized in that one nucleic acid measurement region is disposed at a sufficient distance from the other nucleic acid measurement regions such that the other nucleic acid measurement regions do not enter an irradiation region. | 2014-02-06 |
20140038275 | Pharmacology Bioassays for Drug Discovery, Toxicity Evaluation and in vitro Cancer Research Using a 3D Nanocellulose Scaffold and Living Tissue - The present invention relates to pharmacology bioassays used in drug discovery, drug screening and toxicity evaluations. More specifically, the present invention relates to novel systems and methods used for production and control of 3-D architecture and morphology of living tissues and organs produced by mammalian cells using 3D porous scaffolds based on nano-cellulose. The resultant nano-cellulose based structures are useful as tools in high throughput assays for drugs. More particularly, embodiments of the present invention relate to systems and methods for evaluating a drug that comprise a microtiter plate comprising a plurality of wells, each well comprising: a 3D non-biodegradable, inert, nano-cellulose scaffold; and optionally cells capable of forming living tissue or organs; and optionally a drug having a biological activity of interest; and optionally a detector capable of detecting the biological activity in a high throughput format. | 2014-02-06 |
20140038276 | REACTION VESSEL - We provide a miniature, disposable reactor vessel for bioprocessing. Embodiments include a sealed vessel surrounding a filter through which spent media may be preferentially removed relative to culture cells. Preferred embodiments include an impeller shaft that is contained within the vessel and passes through the filter assembly. The impeller shaft may be engaged magnetically with a drive shaft. Combinations of these reactor vessels and methods of their use are also described. | 2014-02-06 |
20140038277 | INTEGRATED MICROBIAL COLLECTOR - A system for real-time sizing of fluid-borne particles is disclosed. The system further determines, in real time, whether the detected particles are biological or non-biological. As the fluid is being tested, it is exposed to a microbe collection filter which is cultured to determine the type of microbes present in the fluid being tested. | 2014-02-06 |
20140038278 | METHODS FOR GASIFICATION OF CARBONACEOUS MATERIALS - The present disclosure is generally directed to process of gasification of carbonaceous materials to produce synthesis gas or syngas. The present disclosure provides improved methods of gasification comprising adding a molecular oxygen-containing gas and optionally adding water into said gasifier. This disclosure is also directed to process of production of one or more alcohols from said syngas via fermentation or digestion in the presence of at least one microorganism. | 2014-02-06 |
20140038279 | CELL CULTURE SYSTEM - The embodiments of the invention described herein relate to systems and methods for culturing and/or maintaining intestinal cells, tissues and/or organoids in vitro. The cells, tissues and/or organoids cultured according to the methods and systems described herein can mimic or reproduce natural intestinal epithelial structures and behavior as well as support co-culture of intestinal microflora. | 2014-02-06 |
20140038280 | MICROCHIP FOR NUCLIEIC ACID AMPLIFICATION REACTION - There is provided a microchip for a nucleic acid amplification reaction including a reagent accommodation region including a solid-phase reagent and a particle, the solid-phase reagent containing a substance necessary for a nucleic acid amplification reaction. | 2014-02-06 |
20140038281 | SYSTEM FOR CARGO DELIVERY INTO THE CELLS - The present invention relates to a system for intracellular cargo delivery, named NickFect, comprising at least one component A, which is attached covalently to cell penetrating peptide B and/or peptide or non-peptide construct C. The said delivery system NickFect relates to chemically modified new cell-penetrating peptides (CPP) non-covalently or covalently complexed with cargo for efficient cellular. | 2014-02-06 |
20140038282 | MODIFIED VITAMIN K-DEPENDENT POLYPEPTIDES - The invention provides vitamin K-dependent polypeptides with enhanced membrane binding affinity. These polypeptides can be used to modulate clot formation in mammals. Methods of modulating clot formation in mammals are also described. | 2014-02-06 |
20140038283 | Oocyte Separation And Collection System - An apparatus for separating and collecting one or more oocytes from a fluid mixture includes a chamber inserted into a hollow elongate tube. An inlet channel receives an injected fluid mixture of oocytes and extraneous fluids and passes the fluid mixture to the hollow elongate tube. A separation unit positioned within the chamber includes a porous filter that selectively filters the fluid mixture to retain the oocytes in the hollow elongate tube, and a propeller for agitating the extraneous fluids for allowing unobstructed passage of the extraneous fluids into an outlet channel and for compactly collecting fibrin elements from the extraneous fluids. A flow of the extraneous fluids rotates the propeller on application of a vacuum suction force. Furthermore, the chamber includes an outlet channel for allowing passage of the extraneous fluids separated from the fluid mixture out of the chamber, and retaining the oocytes in the hollow elongate tube. | 2014-02-06 |
20140038284 | Mammalian Cell Lines for Increasing Longevity and Protein Yield from a Cell Culture - Disclosed are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. Cells transfected with an apoptosis-inhibiting gene or vector, such as a triple mutant Bcl-2 gene, can survive longer in culture, resulting in extension of the state and yield of protein biosynthesis. Such transfected cells exhibit maximal cell densities that equal or exceed the maximal density achieved by the parent cell lines. Transfected cells can also be pre-adapted for growth in serum-free medium, greatly decreasing the time required to obtain protein production in serum-free medium. In certain methods, the pre-adapted cells can be used for protein production following transformation under serum-free conditions. The method preferably involves eukaryotic cells, more preferably mammalian cells. | 2014-02-06 |
20140038285 | METHOD OF CORRELATED MUTATIONAL ANALYSIS TO IMPROVE THERAPEUTIC ANTIBODIES - A method of improving antibody manufacturability or developability through a computational approach. | 2014-02-06 |
20140038286 | CODON OPTIMIZED SODIUM-IODIDE SYMPORTER GENE AND ITS USE - The present disclosure provides a polynucleotide which is codon optimized for the efficient expression in a eukaryotic cell, a plasmid and a eukaryotic cell comprising the same. The modification resulted in the efficient expression of NIS in eukaryotic cells and the enhancement of the function of NIS by glycosylation. Thus modified polynucleotide encoding NIS of the present disclosure is useful as imaging reporter for gene, viral and/or cell based therapies. | 2014-02-06 |
20140038287 | Fc Receptor-Binding Polypeptides with Modified Effector Functions - Disclosed are processes for producing a variant polypeptide (e.g. antibodies) having modified binding characteristics for human Fc gamma receptor IIA (CD32A) leading to increased inhibition of proinflammatory mediators while retaining binding to a target antigen via its Fv portion, which processes comprise altering the polypeptides by substitution of at least two amino acid residues at EU position 325, 326 or 328 of a human IgG CH2 region for a sequence selected from SAAF, SKAF, NAAF and NKAF. Also disclosed are molecules, particularly polypeptides, more particularly immunoglobulins (e.g. antibodies) that include a variant CDR3 region, wherein the variant CDR3 region includes at least one amino acid modified relative to a wild-type CDR3 region. The polypeptides that can be generated according to the methods of the invention are highly variable, and they can include antibodies and fusion proteins that contain an Fc region or a biologically active portion thereof. | 2014-02-06 |
20140038288 | CYTOTOXIC RIBONUCLEASE VARIANTS - Cytotoxic variants of human ribonuclease 1 (RNase 1) identified through analysis of the interaction between RNase 1 and the human ribonuclease inhibitor (hRI) as defined by the three dimensional (3-D) atomic structure of the RNase1 hRI complex are disclosed. Also disclosed is the 3-D structure of the hRI.RNase 1 complex and methods for designing and using the RNase 1 variants. | 2014-02-06 |
20140038289 | NONADHESIVE SPHERE CELL CULTURE CONTAINER, SYSTEM, AND METHOD - The present invention provides a nonadhesive sphere cell culture container, system and method, which is performed by coating a gel film on the bottom of the culture container, and then by immersing the liquid medium containing cells on the gel film to cultivate cells as the sphere cells. Abundant cancer stem cells with sphere formation can be harvested by the invention with advantages of marked reduction of cost, time-saving (takes only 7-10 days for spheres formation), and without additional separation technique. | 2014-02-06 |
20140038290 | EXTRACELLULAR MATRICES WHICH CAN BE USED AS SCAFFOLD FOR LIVING CELLS - The present invention discloses an extracellular matrix comprising a modified polysaccharide consisting of repeating disaccharide units whereby in at least 11% of the disaccharide units one primary alcohol group is oxidized into a carboxylic acid. | 2014-02-06 |
20140038291 | METHODS FOR REPROGRAMMING CELLS AND USES THEREOF - Described herein are reprogrammed cells, and methods for cell dedifferentiation, transformation and eukaryotic cell reprogramming. Also descried are cells, cell lines, and tissues that can be transplanted in a patient after steps of in vitro dedifferentiation and in vitro reprogramming. In particular embodiments the cells are Stem-Like Cells (SLCs), including Neural Stem-Like Cells (NSLCs), Cardiac Stem-Like Cells (CSLC), Hematopoietic Stem-Like Cells (HSLC), Pancreatic Progenitor-Like Cells, and Mesendoderm-like Cells. Also described are methods for generating these cells from human somatic cells and other types of cells. Also provided are compositions and methods of using of the cells so generated in human therapy and in other areas. | 2014-02-06 |
20140038292 | Tol1 FACTOR TRANSPOSASE AND DNA INTRODUCTION SYSTEM USING THE SAME - An object is to provide a Tol1 element transposase and a use thereof. Provided is a Tol1 element transposase containing (a) a protein having the amino acid sequence of SEQ ID No: 1 or (b) a protein having an amino acid sequence homologous to the amino acid sequence of SEQ ID NO: 1 and having an enzymatic activity for transferring Tol1 element. Further, provided is a polynucleotide encoding the transposase and an expression construct containing the polynucleotide therein. The present invention also provides a DNA introduction system including (a) a donor factor having such a structure that a desired DNA is inserted in a transposase gene-defected Tol1 element and (b) a helper factor containing the transposase or the polynucleotide. | 2014-02-06 |
20140038293 | METHODS FOR THE PRODUCTION OF IPS CELLS USING NON-VIRAL APPROACH - Methods and composition of induction of pluripotent stem cells and other desired cell types are disclosed. For example, in certain aspects methods for generating essentially vector-free induced pluripotent stem cells are described. Furthermore, the invention provides induced pluripotent stem cells and desired cell types essentially free of exogenous vector elements with the episomal expression vectors to express differentiation programming factors. | 2014-02-06 |
20140038294 | SYSTEMS AND METHODS FOR ENGINEERING NUCLEIC ACID CONSTRUCTS USING SCORING TECHNIQUES - Systems and methods are provided for defining a nucleic acid construct for integration at locus L of an organism. Nucleic acid requests are received, each such request specifying a genetic change to L. The request are expanded into component polynucleotides which are then arranged into {AR | 2014-02-06 |
20140038295 | BILIVERDIN FROM A NON-ANIMAL SOURCE - Methods for producing biliverdin in a microorganism, methods for producing biliverdin from a non-animal source, cells for producing biliverdin and methods for producing cells for producing biliverdin are disclosed. | 2014-02-06 |
20140038296 | METABOLIC ENGINEERING OF MICROBIAL ORGANISMS - Microbial strains with desirable carbohydrate productions characteristics and methods of making and using the same are provided herein. | 2014-02-06 |