| 03rd week of 2011 patent applcation highlights part 39 |
| Patent application number | Title | Published |
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| 20110014617 | Methods and Systems for Detecting Nucleic Acids - Methods and kits for detecting a target nucleic acid in a sample are described. In some embodiments, the sample to be analyzed includes a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising nuclease activity that can cleave the hybridized hybridization probe to thereby release a labeled probe fragment. In some embodiments, the sample can then be contacted with a solid support comprising surface bound capture probes which can hybridize to the labeled probe fragment(s). These capture probes more readily bind to the probe fragment(s) than to the intact hybridization probe. The label can then be detected on the support surface. In this manner, improved discrimination between the probe fragments and the intact hybridization probes can be achieved. | 2011-01-20 |
| 20110014618 | DETECTION OF NUCLEIC ACIDS AND PROTEINS - The invention generally relates to methods for detecting a target nucleic acid and a target protein in a single assay. | 2011-01-20 |
| 20110014619 | REACTION TREATMENT APPARATUS AND REACTION TREATMENT METHOD - Provided is a reaction treatment apparatus in which, in a case of mixing a plurality of solutions in a microchip used in a biochemical reaction system, an electric field generation area for changing solute concentration distribution in the solutions is provided in a solution upstream fluid path. Diffusion between the solutions is accelerated by bringing an area with a high solute concentration into contact with another solution. This may shorten a time required for mixing the solutions. | 2011-01-20 |
| 20110014620 | METHODS FOR IDENTIFICATION OF BONE ANABOLIC AGENTS - The present invention is related to methods for identifying bone anabolic agents and factors, identifying pathways that promote proliferation of osteoblasts for bone growth and/or repair, and for identifying new therapeutic targets for treatments for osteoporosis and other bone degenerative disorders characterized by osteopenia. | 2011-01-20 |
| 20110014621 | DETECTING MULTINUCLEOTIDE REPEATS - Methods of determining the length of a multinucleotide repeat region in a target nucleic acid are provided herein which include labeling amplified target nucleic acids with a target detection label independent of the number of multinucleotide repeats and a repeat-detection label proportional to the number of multinucleotide repeats, wherein the two types of labels are each independently incorporated in the amplified target nucleic acids during the amplifying or after the amplifying; binding the amplified target nucleic acids to a capture probe specific for the amplified target nucleic acids; detecting the target detection label associated with the capture probe to produce a first signal; detecting the repeat-detection label associated with the capture probe to produce a second signal; and determining a ratio of the first signal and the second signal, wherein the ratio is indicative of the length of the multinucleotide repeat region in the target nucleic acid. | 2011-01-20 |
| 20110014622 | GENETIC REFERENCE MATERIALS - The invention provides a genetic reference standard with at least one human genetic reference sequence (having a human DNA sequence containing at least one genetic variant whose presence in the DNA of a human subject is indicative of a pathological condition, a predisposition to a pathological condition, or a predisposition to an adverse reaction to external stimuli, or is indicative of a patient's likely response to a therapeutic intervention, i.e. a variant used in pharmacogenomic analysis) cloned into a non-mammalian animal cell line. There are also provided such reference standards where the human DNA is targeted to specific location in the host genome, using homologous recombination. The invention further provides a method of detecting a genetic variant using such reference standards. | 2011-01-20 |
| 20110014623 | TAGGED OLIGONUCLEOTIDES AND THEIR USE IN NUCLEIC ACID AMPLIFICATION METHODS - The present invention provides nucleic acid amplification methods that desirably reduce or eliminate false positive amplification signals resulting from contaminating biological material, e.g., nucleic acid, that may be present in one or more reagents used in an amplification reaction and/or that may be present in the environment in which an amplification reaction is performed. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in amplification reactions, and the environment in which an amplification reaction is performed, are free of bacterial or other nucleic acid contamination that may yield false positive results. | 2011-01-20 |
| 20110014624 | Methods For Identifying Cells Suitable For Large-Scale Production of Recombinant Proteins - The present invention provides methods of identifying a clonal population of cells suitable for large-scale production of a protein of interest. The invention further provides methods for high-throughput screening for genetic rearrangements in the gene encoding the protein of interest, whereby the absence of a deletion in the gene encoding the protein of interest indicates that the cell is suitable for large-scale production of the protein of interest. | 2011-01-20 |
| 20110014625 | System and Method for Determining the Health of a Subject Using Polymorphic Risk Markers - A system and method for predicting the health of a subject comprising obtaining nucleic acid sequence data about the subject. Identifying at least one polymorphic risk marker associated with a change in promoter methylation of a gene associated with lung cancer; and predicting the health of the subject from a presence of at least one polymorphic risk marker identified and kits associated therewith. | 2011-01-20 |
| 20110014626 | METHODS FOR MEASURING THE INSULIN RECEPTOR ALPHA SUBUNIT - Presence of free insulin receptor α-subunit in blood was discovered. Furthermore, methods for measuring the insulin receptor α-subunit was provided, the method comprising the steps of contacting the insulin receptor α-subunit in a blood sample with an antibody recognizing the insulin receptor α-subunit, and detecting the binding between the two. Measurement of the free insulin receptor α-subunit in the blood is useful for evaluating risk factors for diabetes. | 2011-01-20 |
| 20110014627 | METHOD FOR DETERMINING ANTAGONIST ACTIVITY TO A CYTOKININ RECEPTOR - The present invention provides a method for analyzing agonist-activity to a cytokinin receptor, which comprises (1) bringing an examinee substance into contact with a transformed cell into which DNA coding the cytokinin receptor is introduced and (2) measuring the existence or the quantity of intracellular signal transduction from the cytokinin receptor expressed in the transformed cell, and, a method for analyzing antagonist activity to a cytokinin receptor, which comprises (1) bringing an examinee substance and a substance having agonist-activity to the cytokinin receptor into contact with a transformed cell into which DNA coding the cytokinin receptor is introduced and (2) measuring the existence or the quantity of intracellular signal transduction from the cytokinin receptor expressed in the transformed cell, and the like. | 2011-01-20 |
| 20110014628 | Identification of Surface-Associated Antigens for Tumor Diagnosis and Therapy - An isolated truncated desmoglein 4 (DSG4) polypeptide splice variant of the invention is characterized by an amino acid sequence that lacks a region encoded before exon 9 or beyond exon 10 of the DSG4 gene having the polynucleotide sequence of SEQ ID NO: 75. Also disclosed is a method of diagnosing a cancer, or monitoring the course thereof, in a patient. The method comprises detecting in a tissue sample of a patient the expression of a tumor-associated antigen comprising the extracellular domain of a DSG4 polypeptide encoded by a DSG4 gene having the polynucleotide sequence of SEQ ID NO: 75, or a truncated DSG4 polypeptide splice variant characterized by an amino acid sequence that lacks a region encoded before exon 9 or beyond exon 10 of the DSG4 gene. | 2011-01-20 |
| 20110014629 | METHODS FOR IMPROVING THE RECOVERY OF TROPONIN I AND T IN MEMBRANES, FILTERS AND VESSELS - A method to facilitate recovery troponin I and/or troponin T from a sample comprising addition of troponin C to the sample or to a surface from which the troponin I and/or troponin T are recovered. | 2011-01-20 |
| 20110014630 | Fluorescent Carbon Nanoparticles - Disclosed are photoluminescent particles. The particles include a core nano-sized particle of carbon and a passivation agent bound to the surface of the nanoparticle. The passivation agent can be, for instance, a polymeric material. The passivation agent can also be derivatized for particular applications. For example, the photoluminescent carbon nanoparticles can be derivatized to recognize and bind to a target material, for instance a biologically active material, a pollutant, or a surface receptor on a tissue or cell surface, such as in a tagging or staining protocol. | 2011-01-20 |
| 20110014631 | METHOD FOR DIAGNOSING ACUTE CORONARY SYNDROME - This invention concerns a bioaffinity assay for quantitative determination in a sample of free PAPP-A, defined as the pregnancy associated plasma protein A (PAPP-A) that is not complexed to the proform of major basic protein (proMBP), wherein
| 2011-01-20 |
| 20110014632 | ASSAYS FOR CLINICAL ASSESSMENTS OF RHEUMATOID ARTHRITIS - The disclosure provides methods of detecting autoantibodies present in the serum of subjects suffering from rheumatoid arthritis. The methods use capture probes and detection probes that can bind to the antifilaggrin autoantibodies or other epitope related autoantibodies. The presence, absence, and/or amount of the autoantibody complex may be detected, wherein the presence of the complex may indicate a positive diagnosis of rheumatoid arthritis. | 2011-01-20 |
| 20110014633 | ELECTRIC ANALYSIS METHOD - Disclosed is an analysis method comprising the steps of: | 2011-01-20 |
| 20110014634 | NUCLEIC ACIDS ENCODING T2R, A NOVEL FAMILY OF TASTE RECEPTORS - The invention provides isolated nucleic acid and amino acid sequences of taste cell specific G-protein coupled receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of taste cell specific G-protein coupled receptors. | 2011-01-20 |
| 20110014635 | MARKER PEPTIDE FOR ALZHEIMER'S DISEASE - To provide a peptide obtainable by cleaving an N-terminal region and a C-terminal region of Alcadein α, Alcadein β, or Alcadein γ; and capable of being a diagnostic marker for Alzheimer's disease. It is possible to detect Alzheimer's disease at an early stage without burdening subjects to be tested by using the peptide as a diagnostic marker. | 2011-01-20 |
| 20110014636 | METHOD OF DETECTING LEUKEMIC CELL - [Problems] To provide a marker which is useful in diagnosing leukemia and a method of using the same. | 2011-01-20 |
| 20110014637 | Method For Determining The Sensitivity Of Patients Suffering From A Cancer Disease To Biological Therapy - The invention relates to a method for determining the sensitivity of patients suffering from a cancer disease towards targeted biological therapy based on the inhibition of signaling pathways of the members of HER family (e.g., HER-1, HER-2, HER-3 and HER-4) by determining the expression of the biomarker S6 kinase or its post-translationally modified form or of the biomarkers of the activation of S6 kinase or their post-translationally modified forms in the tumor. | 2011-01-20 |
| 20110014638 | SOLUBLE FAS IN ACUTE CORONARY SYNDROMES DIAGNOSIS - The present technology provides methods and materials for the early clinical detection of acute coronary syndromes in a subject. The methods and materials comprise measuring the amount of soluble Fas in a sample obtained from a subject, comparing the amount of soluble sFas with a reference value and detecting the presence of acute coronary syndromes. | 2011-01-20 |
| 20110014639 | HYBRIDOMA CELL LINE PRODUCING MONOCLONAL ANTIBODY AGAINST FOOT-AND-MOUTH DISEASE VIRUS, THE MONOCLONAL ANTIBODY THEREFROM, IMMUNOASSAY REAGENT AND KIT, AND IMMUNOASSAY METHOD - Provided herein are a hybridoma cell line producing monoclonal antibody against foot-and-mouth disease virus (FMDV), the monoclonal antibody therefrom, reagent and kit for ELISA, and immunoassay method. The hybridoma cell line is produced by cell fusion of a parental cell and a myeloma cell line and has the same characteristics as the cell line whose strain designation is CmA40 and deposition number is ATCC (To be Provided). The parental cell is a splenocyte isolated from the spleen of a mouse immunized by an antigen derived from a 3ABC non-structural protein (NSP) of FMDV. The antigen used here is expressed by a prokaryotic cell. The monoclonal antibody produced by the hybridoma cell line can specifically recognize a 3ABC polypeptide and does not cross-react with an antiserum of swine vesicular disease virus. | 2011-01-20 |
| 20110014640 | BLOOD COAGULATION ANALYZER, BLOOD COAGULATION ANALYSIS METHOD, AND COMPUTER PROGRAM PRODUCT - A blood coagulation analyzer is provided by which, while the measurement using reagent for measuring a Fbg (fibrinogen concentration) is being minimized, the result of a measurement such as a PT measurement can be used to accurately acquire a fibrinogen concentration. The blood coagulation analyzer ( | 2011-01-20 |
| 20110014641 | METHOD FOR MEASURING AROMATASE ACTIVITY - The present invention relates to compounds useful for measuring aromatase activity. The invention further provides methods for measuring aromatase activity and for screening test agents which modulate aromatase activity. A kit is also provided for use in such screening methods. | 2011-01-20 |
| 20110014642 | METHOD OF SCREENING FOR COMPOUNDS THAT CAN BE USED FOR THE TREATMENT OF RESPIRATORY CONDITIONS - The present invention relates to the use of a screening method for identifying candidate molecules that can be used for the treatment of respiratory conditions in a mammal, wherein said screening method comprises a step which comprises determining whether the functional activity of a TASK-2 polypeptide in the presence of a test molecule is decreased or eliminated compared with the functional activity of said TASK-2 polypeptide in the absence of said test molecule, the test molecule being considered to be a candidate molecule when it decreases or eliminates said functional activity. | 2011-01-20 |
| 20110014643 | Neuronal Cells Cultured On Microparticles and Methods of Using Same - The present invention provides methods for culturing neuronal cells for transplantation into a subject. The methods include culturing neuronal cells with microparticles to provide a microparticle and neuronal cell culture composition, wherein the microparticles are coated with a compound that provides for attachment of neuronal cells. The present invention also provides methods of screening the cultured neuronal cells as well as kits and systems for use in the same. | 2011-01-20 |
| 20110014644 | METHODS FOR PREDICTING A CANCER PATIENT'S RESPONSE TO ANTIFOLATE CHEMOTHERAPY - The present invention provides methods for individualizing therapy for cancer treatment, and particularly for evaluating a patient's responsiveness to one or more antifolate therapeutic agents prior to treatment with such agents. Particularly, the invention provides an in vitro chemoresponse assay for predicting a patient's response to an antifolate agent, such as pemetrexed or methotrexate. | 2011-01-20 |
| 20110014645 | METHOD FOR DETERMINING A COMPLETE BLOOD COUNT ON A WHITE BLOOD CELL DIFFERENTIAL COUNT - Systems and methods analyzing body fluids such as blood and bone marrow are disclosed. The systems and methods may utilize an improved technique for applying a monolayer of cells to a slide to generate a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and methods for utilizing multi color microscopy for improving the quality of images captured by a light receiving device. | 2011-01-20 |
| 20110014646 | SAMPLE PREPARATION APPARATUS AND SAMPLE PREPARATION METHOD, AND CELL ANALYZER AND CELL ANALYSIS METHOD - A sample preparation apparatus comprising: a detector for detecting a predetermined cell included in a biological sample; a sample preparation section for preparing a measurement sample from the biological sample and a predetermined reagent; and a controller configured to generate, based on a detection result by the detector, concentration information reflecting a concentration of the predetermined cell in the biological sample and to control, based on the concentration information, the amount of the biological sample supplied to the sample preparation section. | 2011-01-20 |
| 20110014647 | System and Method for Analyzing Samples Labeled with 5, 10, 15, 20 Tetrakis (4-Carboxyphenyl) Porphine (TCPP) - One embodiment of the present invention provides for a method of determining if a sputum sample contains dysplastic or carcinomic cells by obtaining a sputum sample containing cells. The sputum sample is labeled with TCPP to stain cells suspected to be dysplastic or carcinomic. The labeled sputum sample is excited with an excitation wavelength of light of about 475 nm+/−30 nm and emission at about 560 nm+/−30 nm is detected from cells identified to be macrophages. An imager focuses on the plasma membrane of one or more cells suspected to be dysplastic or carcinomic and emission at about 655 nm+/−30 nm, if present, is detected for TCPP labeled cells of the sputum sample after focusing on the plasma membrane of the cells of the sputum sample. Photon flux for each pixel of a sensor is measured to obtain a value for the imaged cell. The measured value is scored to determine if a cell is cancerous or dysplastic. | 2011-01-20 |
| 20110014648 | A MICROBIOLOGICAL PRODUCTION METHOD AND EQUIPMENT FOR ITS USE - When using the equipment and method in accordance with this invention, it is possible to implement biotechnological production e.g. in biorefineries in such a way that it is possible to make use of the phenomena of the gas-liquid interface and interfaces between other phases by means of a moving process solution and gas led into it. This way, production and post-treatment methods can also be integrated, and desired acceleration of reactions and cost reductions can be achieved. | 2011-01-20 |
| 20110014649 | METHOD FOR PRODUCTION OF N36-BINDING PEPTIDE - The present invention relates to the production of an N36-binding peptide at low cost and in a large quantity utilizing a microorganism. More specifically, the present invention relates to a method for producing an N36-binding peptide comprising introducing a recombinant vector into which a DNA molecule encoding an N36-binding peptide that binds to an N36 protein derived from a retrovirus that causes immunodeficiency in a mammal has been incorporated into | 2011-01-20 |
| 20110014650 | IN VIVO UNNATURAL AMINO ACID EXPRESSION IN THE METHYLOTROPHIC YEAST PICHIA PASTORIS - The invention provides orthogonal translation systems for the production of polypeptides comprising unnatural amino acids in methylotrophic yeast such as | 2011-01-20 |
| 20110014651 | METHOD FOR HIGH-LEVEL SECRETORY PRODUCTION OF PROTEIN - This invention provides a means for high-level secretory production of a protein, and, in particular, a protein having a complicated structure such as an antibody, in a host cell such as a yeast cell. This invention provides a method for high-level secretory production of a foreign protein with the use of a transformed host cell having one or more types of chaperone protein genes and via suppression of O sugar chain inherent to a host cell such as a yeast cell. | 2011-01-20 |
| 20110014652 | Norovirus and sapovirus antigens - Immunogenic compositions that elicit immune responses against Norovirus and Sapovirus antigens are described. In particular, the invention relates to polynucleotides encoding one or more capsid proteins or other immunogenic viral polypeptides from one or more strains of Norovirus and/or Sapovirus, coexpression of such immunogenic viral polypeptides with adjuvants, and methods of using the polynucleotides in applications including immunization and production of immunogenic viral polypeptides and viral-like particles (VLPs). Methods for producing Norovirus- or Sapovirus-derived multiple epitope fusion antigens or polyproteins and immunogenic compositions comprising one or more immunogenic polypeptides, polynucleotides, VLPs, and/or adjuvants are also described. The immunogenic compositions of the invention may also contain antigens other than Norovirus or Sapovirus antigens, including antigens that can be used in immunization against pathogens that cause diarrheal diseases, such as antigens derived from rotavirus. | 2011-01-20 |
| 20110014653 | PRODUCTION OF ANTIBODIES IN AVIAN CELLS - The present invention relates generally to novel methods of producing immunoglobulin polypeptides. More specifically, the invention relates to methods of inserting immunoglobulin-encoding transgenes into avian cells for immunoglobulin production. In one embodiment, the transgenes include at least two immunoglobulin-encoding nucleic acid sequences and an internal ribosome entry site (IRES). | 2011-01-20 |
| 20110014654 | CELL FOR USE IN PROUDUCTION OF HETEROPROTEINS AND PRODUCTION METHOD USING THE SAME - The present invention provides a method capable of producing a protein efficiently. | 2011-01-20 |
| 20110014655 | Selection of host cells expressing protein at high levels - The invention provides a DNA molecule comprising a multicistronic transcription unit coding for i) a selectable marker polypeptide functional in a eukaryotic host cell, and for ii) a polypeptide of interest, the polypeptide of interest having a translation initiation sequence separate from that of the selectable marker polypeptide, characterized in that the coding sequence for the polypeptide of interest is downstream from the coding sequence for the selectable marker in the multicistronic transcription unit, and the nucleic acid sequence coding for the selectable marker polypeptide comprises a mutation that decreases the translation efficiency of the selectable marker in a eukaryotic host cell. The invention also provides methods for obtaining host cells expressing a polypeptide of interest, the host cells comprising the DNA molecules of the invention. The invention further provides the production of polypeptides of interest, comprising culturing host cells comprising the DNA molecules according to the invention. | 2011-01-20 |
| 20110014656 | SOLUBLE IL-17RA/RC FUSION PROTEINS AND RELATED METHODS - Disclosed are antagonists of IL-17A and IL-17F. The antagonists are based on soluble IL-17RA and IL-17RC fusion proteins, including hybrid soluble receptors comprising portions of both IL-17RC and IL-17RA (“IL-17RC/IL-17RA”). Such antagonists serve to block, inhibit, reduce, antagonize or neutralize the activity of IL-17F, IL-17A, or both IL-17A and IL-17F. Also disclosed are methods of using such antagonists for treating disease, particularly inflammatory diseases mediated at least in part by IL-17A and/or IL-17F. | 2011-01-20 |
| 20110014657 | Method for sequencing a polynucleotide template - The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template. | 2011-01-20 |
| 20110014658 | COMPOSITIONS AND METHOD FOR STORAGE OF NUCLEIC ACID FROM BODILY FLUIDS - The present invention provides an aqueous composition and method for extracting nucleic acid from a sample of bodily fluid, such as saliva, such that the nucleic acid within said sample remains stable for at least fourteen days at room temperature. The composition permits direct use of the extracted and stored DNA in an amplification reaction without further processing. | 2011-01-20 |
| 20110014659 | ISOLATION OF UNKNOWN REARRANGED T-CELL RECEPTORS FROM SINGLE CELLS - Disclosed herein are methods and materials for isolating and identifying T cell receptors from single cells. In some embodiments, genomic DNA from a single T cell is isolated using whole genome amplification (WGA). A series of PCR reactions is carried out to enrich the genomic template for sequences encoding the TCR alpha and beta chains, and then to isolate the sequences encoding the TCR alpha and beta chains. | 2011-01-20 |
| 20110014660 | THERMOSTABLE DNA POLYMERASE FROM PALAEOCOCCUS FERROPHILUS - There is provided a polypeptide having thermostable DNA polymerase activity and comprising or consisting of an amino acid sequence with at least 90% identity to | 2011-01-20 |
| 20110014661 | METHOD OF PRODUCING SIALYLATED OLIGOSACCHARIDES - The present invention relates to a method for the large scale in vivo synthesis of sialylated oligosaccharides, culturing a microorganism in a culture medium, optionally comprising an exogenous precursor such as lactose, wherein said microorganism comprises heterologous genes encoding a CMP-Neu5Ac synthetase, a sialic acid synthase, a GlcNAc-6-phosphate 2 epimerase and a sialyltransferase, and wherein the endogenous genes coding for sialic acid aldolase (NanA) and for ManNac kinase (NanK) have been deleted or inactivated. The invention also relates to these micoorganisms which are capable of producing internally activated sialic acid. | 2011-01-20 |
| 20110014662 | Methods for producing hyaluronan in a recombinant host cell - The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a | 2011-01-20 |
| 20110014663 | METHOD FOR PRODUCING AN L-AMINO ACID - An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability in a medium containing a processed product of a microalga which promotes production and accumulation of the L-amino acid by the bacterium. The process product is produced by disrupting the culture of the microalga, and/or extracting the culture of the microalga, or fractionating the culture of the microalga or the disrupted culture. The processed product contains a mixture of organic substances produced by the microalga, a hydrolysate of the disrupted microalga culture, and/or an extract or fractionation product of the microalga culture. The processed product can also contain a saccarification product of starch or a hydrolysate of fats and oils. The bacterium is cultured to produce and accumulate the L-amino acid in culture, and the L-amino acid is collected from the culture. | 2011-01-20 |
| 20110014664 | Fermentation Process for Higher Yield Coefficient of Lipase-Inhibitor with Respect to Consumed Fatty Acid - The invention provides a process for the production of lipase inhibitors via an improvised fermentation process characterized in that a combinatorial feeding of linoleic acid or its esters or salts thereof and an omega-9 fatty acid, preferably oleic acid and/or its derivatives is employed during said process resulting in an improved yield co-efficient, productivity further providing ease of operation. | 2011-01-20 |
| 20110014665 | Production of Oil in Microorganisms - The invention provides methods of cultivating oil-bearing microbes using cellulosic material. Also provided are microorganisms containing one or more exogenous genes that facilitate the use of cellulosic materials as a feedstock. Also provided are microorganisms and methods for manufacturing non-alcohol-based fuels and fuel feedstocks through a process of converting cellulosic materials into oils. | 2011-01-20 |
| 20110014666 | POLYPEPTIDE HAVING GLYOXALASE III ACTIVITY, POLYNUCLEOTIDE ENCODING THE SAME AND USES THEREOF - The present invention relates to a novel polypeptide having the enzymatic activity of conversion of methylglyoxal to lactic acid in a single step (known as glyoxalase III activity), a polynucleotide having a nucleotide sequence encoding such polypeptide and uses thereof. | 2011-01-20 |
| 20110014667 | Producing Dicarboxylic Acids Using Polyketide Synthases - The present invention provides for a polyketide synthase (PKS) capable of synthesizing a dicarboxylic acid (diacid). Such diacids include diketide-diacids and triketide-diacids. The invention includes recombinant nucleic acid encoding the PKS, and host cells comprising the PKS. The invention also includes methods for producing the diacids. | 2011-01-20 |
| 20110014668 | ORGANISMS FOR THE PRODUCTION OF CYCLOHEXANONE - A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on C—C bond), a 2-ketocyclohexane-1-carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxyl-CoA transferase, and a 2-ketocyclohexane-1-carboxyl-CoA synthetase. A pathway includes an enzyme selected from a 6-ketocyclohex-1-ene-1-carboxyl-CoA hydrolase (acting on C—C bond), a 6-ketocyclohex-1-ene-1-carboxyl-CoA synthetase, a 6-ketocyclohex-1-ene-1-carboxyl-CoA hydrolase (acting on thioester), a 6-ketocyclohex-1-ene-1-carboxyl-CoA transferase, a 6-ketocyclohex-1-ene-1-carboxyl-CoA reductase, a 6-ketocyclohex-1-ene-1-carboxylate decarboxylase, a 6-ketocyclohex-1-ene-1-carboxylate reductase, a 2-ketocyclohexane-1-carboxyl-CoA synthetase, a 2-ketocyclohexane-1-carboxyl-CoA transferase, a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxylate decarboxylase, and a cyclohexanone dehydrogenase. A pathway includes an adipate semialdehyde dehydratase, a cyclohexane-1,2-diol dehydrogenase, and a cyclohexane-1,2-diol dehydratase. A pathway includes a 3-oxopimelate decarboxylase, a 4-acetylbutyrate dehydratase, a 3-hydroxycyclohexanone dehydrogenase, a 2-cyclohexenone hydratase, a cyclohexanone dehydrogenase and an enzyme selected from a 3-oxopimeloyl-CoA synthetase, a 3-oxopimeloyl-CoA hydrolase (acting on thioester), and a 3-oxopimeloyl-coA transferase. Each these pathways can include a PEP carboxykinase. A method for producing cyclohexanone includes culturing these non-naturally occurring microbial organisms. | 2011-01-20 |
| 20110014669 | Production of 1,4 Butanediol in a Microorganism - A biological method for the conversion of L-glutamate to 1,4-butanediol that involves a decarboxylation step and avoids production of 4-hydroxybutyrate as an intermediate is described. The method includes: (a) conversion of L-glutamate to L-glutamate 5-phosphate; (b) conversion of L-glutamate 5-phosphate to L-glutamate 5-semialdehyde; (c) conversion of L-glutamate 5-semialdehyde to 5-hydroxy-L-norvaline; (d) conversion of 5-hydroxy-L-norvaline to 5-hydroxy-2-oxopentanoate; (e) conversion of 5-hydroxy-2-oxopentanoate to 4-hydroxybutanal; and (f) the conversion of 4-hydroxybutanal to 1,4-butanediol. | 2011-01-20 |
| 20110014670 | ZYMOMONAS WITH IMPROVED XYLOSE UTILIZATION IN STRESS CONDITIONS - Strains of xylose utilizing | 2011-01-20 |
| 20110014671 | METHOD FOR OBTAINING BIOETHANOL FROM SORGHUM GRAIN (SORGHUM BICOLOR L. MOENCH), COMPRISING STEPS INVOLVING DECORTICATION AND HYDROLYSIS WITH PROTEASES - The invention relates to an improved method for obtaining bioethanol from | 2011-01-20 |
| 20110014672 | ISOPRENE PRODUCTION USING THE DXP AND MVA PATHWAY - The invention provides for methods for producing isoprene from cultured cells using various components of the DXP pathway and MVA pathway, or components associated with the DXP pathway and MVA pathway, iron-sulfur cluster-interacting redox polypeptides, and isoprene synthase. The invention also provides compositions that include these cultured cells. | 2011-01-20 |
| 20110014673 | Systems and Methods for Effecting a Physical Change in a Biological Sample - The present invention relates generally to systems and methods for processing a biological sample that result in a physical change, such as reacting two molecules together to form a reaction product or for use in lysing viruses or biological cells for analysis using biological assay systems. As such, the present invention relates both to breaking apart biological species such as viruses and cells, as well as the formation of reactants from one or more reactive species. The sample has a volume in the range from about 1 microliter to 10 milliliters. The sample is processed by applying pressure, and either sonic energy or thermal energy to the sample, wherein the pressure achieved is usually at least 24 atmospheres, and the temperature of the sample is usually raised to at least 50° C. | 2011-01-20 |
| 20110014674 | CARBON NANOTUBE BINDING PEPTIDES - Peptides have been generated that have binding affinity to carbon nanostructures and particularly carbon nanotubes. Peptides of or the invention are generally about twelve amino acids in length. Methods for generating carbon nanotube binding peptides are also disclosed. | 2011-01-20 |
| 20110014675 | Poly zinc finger proteins with improved linkers - Polynucleotides encoding chimeric proteins, and methods for their production and use are disclosed. The chimeric proteins comprise a flexible linker between two zinc finger DNA-binding domains, wherein the linker contains eight or more amino acids between the second conserved histidine residue of the carboxy-terminal zinc finger of the first domain and the first conserved cysteine residue of the amino-terminal zinc finger of the second domain. | 2011-01-20 |
| 20110014676 | PROTEIN STABILIZATION - A method and formulation for temperature stabilization of proteins, such as antibodies, enzymes such as Taq poly-merase, restriction enzymes, and other diagnostic or therapeutic enzymes using a combination of first and second stabilizers. | 2011-01-20 |
| 20110014677 | NOVEL DNA FRAGMENT, RECOMBINANT VECTOR COMPRISING SAME, TRANSFORMANT TRANSFORMED THEREWITH, AND USE THEREOF - A DNA fragment containing a gene which encodes a specific gene regulatory region alone or the gene regulatory region together with a signal peptide; a recombinant vector containing the DNA fragment; a transformant containing the recombinant vector; and a method of producing a recombinant protein by using the transformant. According to the invention, it is possible to produce a protein in a large amount at a high efficiency regardless of the kind of the recombinant protein. | 2011-01-20 |
| 20110014678 | SULFOTRANSFERASE 1E1 SEQUENCE VARIANTS - Isolated sulfotransferase nucleic acid molecules that include a nucleotide sequence variant and nucleotides flanking the sequence variant are described, as well as sulfotransferase allozymes. Methods for determining if a mammal is predisposed to cancer also are described. | 2011-01-20 |
| 20110014679 | DNA REPLICATION FACTORS - A DNA polymerase reaction system which provides high DNA polymerase activity even at a high temperature and at a high salt concentration. A DNA polymerase reaction system that is constructed from a DNA polymerase, a clamp, and a clamp loader without intein sequence, the DNA polymerase being from | 2011-01-20 |
| 20110014680 | CRYSTAL STRUCTURE OF HUMAN JAK3 KINASE DOMAIN COMPLEX AND BINDING POCKETS THEREOF - The present invention relates to human Janus Kinase 3 (JAK3) and JAK3-like binding pockets. The present invention provides a computer comprising a data storage medium encoded with the structure coordinates of such binding pockets. This invention also relates to methods of using the structure coordinates to solve the structure of homologous proteins or protein complexes. In addition, this invention relates to methods of using the structure coordinates to screen for and design compounds, including inhibitory compounds, that bind to JAK3 protein or JAK3 protein homologues, or complexes thereof. The invention also relates to crystallizable compositions and crystals comprising JAK3 kinase domain and JAK3 kinase domain complexed with AMP-PNP. | 2011-01-20 |
| 20110014681 | GLUCOAMYLASE VARIANTS - The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells. | 2011-01-20 |
| 20110014682 | CARBON NANOTUBE BINDING PEPTIDES - Peptides have been generated that have binding affinity to carbon nanostructures and particularly carbon nanotubes. Peptides of or the invention are generally about twelve amino acids in length. Methods for generating carbon nanotube binding peptides are also disclosed. | 2011-01-20 |
| 20110014683 | System and Method for Growing Photosynthetic Cells - Disclosed are a system and method for growing photosynthetic cells in conduit. The system and method supply light, CO2 and nutrients to the cells. The system and method also dampen thermal variations in the conduit. | 2011-01-20 |
| 20110014684 | METHOD FOR WASTEWATER TREATMENT AND WASTEWATER TREATMENT SYSTEM - The invention relates to a method for wastewater treatment utilizing an anaerobic treatment of primary sludge (PS) in a septic tank ( | 2011-01-20 |
| 20110014685 | CELL PROCESSING APPARATUS, SAMPLE PREPARATION APPARATUS, AND CELL ANALYZER - A cell processing apparatus | 2011-01-20 |
| 20110014686 | Method and apparatus for imaging target components in a biological sample using permanent magnets - A system for enumeration of cells in fluids by image cytometry is described for assessment of target populations such as leukocyte subsets in different bodily fluids or bacterial contamination in environmental samples, food products and bodily fluids. Briefly, fluorescently labeled target cells are linked to magnetic particles or beads. In one embodiment, a small, permanent magnet is inserted directly into the chamber containing the labeled cells. The magnets are coated with PDMS silicone rubber to provide a smooth and even surface which allows imaging on a single focal plane. The magnet is removed from the sample and illuminated with fluorescent light emitted by the target cells captured by a CCD camera. In another embodiment, a floater having a permanent magnet allows the target cells to line up along a single imaging plane within the sample solution. Image analysis can be performed with a novel algorithm to provide a count of the cells on the surface, reflecting the target cell concentration of the original sample. | 2011-01-20 |
| 20110014687 | CONNECTION DEVICE AND ANALYZER - A connection device for connecting to a plurality of fluid containers that have openings, and a sample analyzer body for analyzing samples using reagent, is disclosed, which comprises: a plurality of tubular members for passing liquid, the plurality of tubular members being inserted in the fluid containers through the openings of the plurality of fluid containers; a holding member to hold the plurality of tubular members; and a plurality of cover members to be fitted to the opening of the fluid container, wherein the plurality of cover members are arranged in a predetermined positional relationship on the holding member side of the plurality of tubular members; configured with a continuously decreasing cross sectional area toward the fluid container side; and the shape of the cross section of the cover member is substantially the same as the shape of the corresponding opening of the fluid container. | 2011-01-20 |
| 20110014688 | PICO LITER WELL HOLDING DEVICE AND METHOD OF MAKING THE SAME - The present invention broadly comprises a holding device for studying cells comprising at least one cavity adapted to receive cells in a medium consisting essentially of water, the cavity having a substrate and a generally inert wall, wherein the substrate includes a surface for receiving the medium, and wherein the surface includes a multiplicity of pico liter wells and is characterized in that the substrate is substantially translucent and has a refractive index equal to the refractive index of the medium. The invention further comprises a method of making the holding device comprising providing a carrier plate, applying an adhesive layer to the carrier plate, depositing a curable substrate on the adhesive layer, applying a second layer of adhesive to the substrate, attaching a wall structure to the second layer of adhesive, forming a multiplicity of pico liter wells in the substrate, curing the substrate, and removing the template. | 2011-01-20 |
| 20110014689 | Disposable Bio-Reactor System - A disposable bioreactor system with a focus on improving cell culture technology by introducing a disposable bioreactor bag which operates or functions in conjunction with a unique mechanism of external non-rotating gyrating motion that uses gravity in a natural way to make the fluid flow circumferentially in it. The bioreactor is eminently suitable to grow a variety of living cells and has the advantage of tangential flow to avoid clogging of filters. | 2011-01-20 |
| 20110014690 | CORNEA STORAGE CONTAINER TO MAXIMIZE CORNEA HEALTH - An apparatus for shipping, storing, and viewing a cornea. The device offers an improvement to cornea health relative to conventional cornea containers. | 2011-01-20 |
| 20110014691 | METHOD FOR GENERATING PRIMATE CARDIOVASCULAR PROGENITOR CELLS FOR CLINICAL USE FROM PRIMATE EMBRYONIC STEM CELLS OR EMBRYONIC-LIKE STATE CELLS, AND THEIR APPLICATIONS - The present invention is directed to a method for the in vitro preparation of cardiovascular progenitors cells from mammalian embryonic stem cells (ES cells) or mammalian embryonic-like state cells, preferably from primate, wherein said method comprises the use of the CD15 (SSEAI) marker as a positive cardiovascular progenitors differentiation marker. The present invention also claimed the use of a receptor tyrosine kinase inhibitor, particularly the SU5402 or SU11248 in association with the BMP2 for improving the efficiency of the desired differentiation. The present invention is also directed to the use of platelet lysate as foetal animal serum substitute in a culture medium intended to the proliferation or propagation of primate ES cells maintaining their pluripotency feature. Derived compositions or kits in relation with the claimed methods or product obtainable by the claimed methods form also part of the present invention. | 2011-01-20 |
| 20110014692 | METHOD OF DERIVING PROGENITOR CELL LINE - We disclose a method comprising: (a) providing an embryonic stem (ES) cell; and (b) establishing a progenitor cell line from the embryonic stem cell; in which the progenitor cell line is selected based on its ability to self-renew. Preferably, the method selects against somatic cells based on their inability to self-renew. Preferably, the progenitor cell line is derived or established in the absence of co-culture, preferably in the absence of feeder cells, which preferably selects against embryonic stem cells. Optionally, the method comprises (d) deriving a differentiated cell from the progenitor cell line. | 2011-01-20 |
| 20110014693 | Microcarriers For Stem Cell Culture - We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells. | 2011-01-20 |
| 20110014694 | USE OF ENTERIC GLIA - Methods of reducing tissue damage in the nervous system are disclosed. The methods involved administering enteric glial cells to an animal with a nerve injury. Methods of improving locomotor function in animal with a nerve injury are also disclosed. | 2011-01-20 |
| 20110014695 | METHODS FOR OBTAINING ADULT HUMAN OLFACTORY PROGENITOR CELLS - An isolated human olfactory stem cell can be prepared by culturing human tissue from olfactory neuroepithelium to form neurospheres. | 2011-01-20 |
| 20110014696 | METHOD FOR PRODUCING DENDRITIC CELLS - Disclosed are embryonic stem cell-derived dendritic cells, genetically modified immature dendritic cells capable of maturation, as well as methods for the production of such cells. In one embodiment, the cells made be produced by a method comprising the steps of providing a population of embryonic stem cells; culturing the embryonic stem cells in the presence of a cytokine or combination of cytokines which brings about differentiation of the embryonic stem cells into dendritic cells; and recovering the dendritic cells from the culture. In a further embodiment, the cells may be genetically modified. | 2011-01-20 |
| 20110014697 | Method and Kit for Rapid Isolation of Human Foxp3+ Treg Cells - The present invention relates to methods for isolating human forkhead box P3 (Foxp3+) CD4+ regulatory T cells (herein referred to as Foxp3+ Treg cells) from a sample containing (i) peripheral blood mononuclear cells (PBMCs), (ii) a lymphocyte containing fluid, or (iii) a lymphocyte containing tissue, a kit for isolating human Foxp3+ Treg cells, and the use of anti-CD49d antibody for the isolation of human Foxp3+ Treg cells. | 2011-01-20 |
| 20110014698 | USE OF NANOPATTERNED SURFACES AND METHOD FOR ENRICHING OR ISOLATING CELLULAR SUBPOPULATIONS - The invention relates to the use of nanopatterned surfaces. It also relates to a method for enriching or isolating cellular subpopulations. To create a simple, versatile and specific method for enriching or isolating cellular subpopulations from a complex mixture, the invention proposes the use of nanopatterned surfaces for isolating and enriching cellular subpopulations from a complex mixture. | 2011-01-20 |
| 20110014699 | PROSTRATIN ANALOGS, BRYOSTATIN ANALOGS, PRODRUGS, SYNTHETIC METHODS, AND METHODS OF USE - Embodiments of the present disclosure provide for prostratin analogs, bryostatin analogs, prodrugs of prostratin and prostratin analogs, methods of making prostratin analogs, and methods of making prodrugs of prostratin and prostratin analogs, methods of use of prostratin analogs, bryostatin analogs, and prodrugs thereof, and the like. | 2011-01-20 |
| 20110014700 | Methods of Storing and Providing Samples of Cells and Tissue - A cell or tissue sample from a donor is stored in bank wherein the donor can initially determine the sample status to (a) exclusively retain, (b) make public, or (c) optionally make public his cells or tissue. Where the donor opts for optional public storage, the donor may in response to a third party request convert the optional public storage status to public status or private status top thereby share or exclusively retain his cells or tissue. Most preferably, depending on the initial and/or subsequent choice by the donor, an incentive or disincentive is provided. | 2011-01-20 |
| 20110014701 | Protection of Progenitor Cells and Regulation of Their Differentiation - The present invention relates to the use of polysulfated polysaccharides in combination with progenitor cells to improve the viability of the progenitor cells including improving the cryopreservation of the progenitor cells and provides novel compositions, methods and uses. The present invention also relates to the use of polysulfated polysaccharides to regulate the proliferation and differentiation of progenitor cells. | 2011-01-20 |
| 20110014702 | Differentiation of Human Embryonic Stem Cells - The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce cells capable of producing insulin following transplantation into an animal. | 2011-01-20 |
| 20110014703 | Differentiation of Human Embryonic Stem Cells - The present invention provides methods to promote the differentiation of pluripotent stem cells into cells expressing markers characteristic of the pancreatic endocrine lineage that co-express PDX1, NKX6.1, but do not express CDX2 and NGN3. | 2011-01-20 |
| 20110014704 | ISOLATION OF MULTI-LINEAGE STEM CELLS - The present application discloses a method of manipulating a biological sample of cells, which includes multi-lineage stem cells, progenitor cells, other marrow stromal cells: allowing the sample of cells to settle in a container; transferring supernatant from the container to another container; and isolating cells from the supernatant, which has comparatively lower density in the sample. | 2011-01-20 |
| 20110014705 | METHOD AND APPARATUS FOR SEPARATING BIOLOGICAL MATERIALS - According to various embodiments, a system can be provided to separate undifferentiated cells and/or stromal cells from a whole tissue sample. The whole tissue sample can be any appropriate tissue sample obtained directly from a patient. The tissue sample can be obtained during a selected operating procedure for immediate or quick application or re-application to the patient. Accordingly, autologous cells can be obtained intraoperatively for application to a patient substantially soon after obtaining a whole tissue sample. | 2011-01-20 |
| 20110014706 | Arabidopsis thaliana Genome Sequence and Uses Thereof - The present invention relates to nucleic acid sequences from the dicotyldonus plant | 2011-01-20 |
| 20110014707 | Polypeptides having endoglucanase activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. | 2011-01-20 |
| 20110014708 | NUCLEIC ACID FOR USE IN ALGAE AND USE THEREOF - The present invention provides a modified nucleic acid for expressing | 2011-01-20 |
| 20110014709 | Method of Modifying Target Region in Host DNA and Selectable Marker Cassette - A method of modifying a target region in a host DNA using a donor DNA:
| 2011-01-20 |
| 20110014710 | COMPOSITIONS, METHODS AND KITS FOR BIARSENICAL FLUOROPHORE LABELING - Methods, compositions, and kits for labeling tetracysteine-tagged proteins with biarsenical fluorophores with increased specificity, including compositions, methods and kits particularly adapted for labeling of tetracysteine-tagged proteins to be resolved within an electrophoresis gel. | 2011-01-20 |
| 20110014711 | MIX FOR IDENTIFICATION TEST IN THE PROCESS OF QUALITY CONTROL OF THE MEDICINE 'GLYCINE TABLETS FOR SUBLINGUAL APPLYING 0,1G' METHODS OF ITS PREPARATION AND IDENTITY EVALUATION IN THE PROCESS OF QUALITY CONTROL OF THE AFOREMENTIONED MEDICINE - The invention relates to the chemical-pharmaceutical industry and specifically to mix for identification test in the process of quality control of the medicine ‘Glycine tablets for sublingual applying 0.1 g.’ its preparation method and method of identity evaluation in the process of quality control of the mentioned medicine. There is prepared mix containing 50% ethaπτl and porphyrized tablets in a ratio 100:0.5. The method involves dissolution of 1.25 g of porphyrized tablets in 250 ml of 50% ethanol. Process of dissolution takes 20 minutes and is carried out at a temperature of 400 C in the apparatus for dissolving determination at a paddle rotation speed of 200 rpm. After mix is dissolved it is allowed for 10 minutes RT. Method of identification test includes hydro-alcohol mix preparation using 50% ethanol as described before. Then there are selected 4 ml of the mix for light transmission spectrophotometer analysis at a wave length of 700±2 in a cuvet with layer thickness of 10 mm relative to 50% ethanol. Water mix is prepared by dissolving of 2.5 g of porphyrized tablets in 250 ml, of purified water for 20 minutes at a temperature of 370 C. Experiments with water mix and hydro-alcohol mix are similar. Therefore there is determined difference between light transmission coefficients of water mix and hydro-alcohol mix and the obtained value is compared to the limit of 30 to 50%. | 2011-01-20 |
| 20110014712 | METHOD, DEVICE AND APPARATUS FOR MEASURING THE CONCENTRATION OF CREATININE, AND METHOD, DEVICE AND APPARATUS FOR MEASURING THE AMOUNT OF SALT USING THE SAME - A method for measuring a concentration of creatinine includes the steps of: (A) mixing a sample containing creatinine with a creatinine quantitative reagent containing a metal complex of at least one of hexacyanoferrate and hexacyanoruthenate in the absence of picric acid and any enzyme responsive to creatinine, to cause the creatinine to reduce the metal complex; (B) electrochemically or optically measuring the amount of the metal complex reduced in the step (A); and (C) determining the concentration of the creatinine contained in the sample from the amount of the reduced metal complex measured in the step (B). | 2011-01-20 |
| 20110014713 | DETECTION METHOD AND DETERMINATION METHOD FOR DETECTION TARGET - A detection/determination kit with which a substance to be detected or the amount thereof can be rapidly, inexpensively, and easily detected or determined; and a detection/determination method. The kit, which is for detecting a substance to be detected ( | 2011-01-20 |
| 20110014714 | METHOD FOR SELECTIVE LABELING OF PROTEIN PRODUCED BY IN VITRO TRANSLATION BY USING A MARKER FROM TRNA PREPARED BY USING IN VITRO TRANSCRIPTION - The present invention relates to a method for the preparation of a labeling material (labeling agent) usable for non-radioactive selective labeling of proteins produced by in vitro translation with fluorophore or biotin; and a method for selective labeling of proteins produced by in vitro translation using the said labeling material. More specifically, the present invention provides a method for preparing a labeling material usable for selective labeling of a target protein produced only from the gene added to the cell-free protein synthesis reaction mixture by an experimenter without labeling pre-existing proteins in the reaction mixture, for which only one type of tRNA prepared by in vitro transcription is used instead of “total tRNA mixture”. The present invention also provides a method for labeling of the protein produced by in vitro translation by using the labeling material prepared by the above method. According to the present invention, the preparation of a labeling material becomes easier and the protein labeled with the labeling material of the present invention provides much improved signals than the protein labeled with the labeling material of the conventional method prepared by using “total tRNA mixture” as a tRNA material. | 2011-01-20 |
| 20110014715 | Synthesis of Peptide Nucleic Acids Conjugated with Amino Acids and Their Application - This invention relates to a peptide nucleic acid (PNA) oligomer which is conjugated with one or more linear-type amino acid containing a plurality of alkyleneglycols and to a synthesis method thereof. In addition, this invention related to a linear amino acid spacer in a device for detection for detecting a target gene using the PNA oligomers which is fixed on a surface of a functionalized solid support. The linear amino acid spacer contains a plurality of alkyleneglycols and maintains enough space between the solid support and PNA oligomer in the device in order to prevent the interference of the interaction between the PNA oligomer and a target gene. Furthermore, this invention relates to a PNA array, a PNA chip and a gene diagnosis kit whereof sensitivity and specificity are improved by being manufactured with the PNA conjugated with the amino acid spacer. | 2011-01-20 |
| 20110014716 | COMPOSITIONS AND METHODS FOR DIAGNOSING AND TREATING MACULAR DEGENERATION - The present invention relates generally to biomarkers for macular degeneration. In particular, the present invention provides a plurality of biomarkers for monitoring and diagnosing macular degeneration. The compositions and methods of the present invention find use in diagnostic, therapeutic, research, and drug screening applications. | 2011-01-20 |
