01st week of 2009 patent applcation highlights part 47 |
Patent application number | Title | Published |
20090004608 | Detergent For Lithography And Method Of Forming Resist Pattern With The Same - Conventional detergents for lithography which contain a surfactant as an active ingredient should have a reduced surfactant concentration because heightened surfactant concentrations result in dissolution of the resin component of a photoresist composition and hence in a dimensional change of a resist pattern. However, the conventional detergents have had a drawback that such a low concentration unavoidably reduces the ability to inhibit pattern falling and defect occurrence. A detergent for lithography is provided which is an aqueous solution containing (A) at least one member selected among nitrogenous cationic surfactants and nitrogenous ampholytic surfactants and (B) an anionic surfactant. This detergent retains a low surface tension even when it has a low concentration. It is effective in inhibiting pattern falling and defect occurrence. It can also inhibit resist patterns from fluctuating in dimension. | 2009-01-01 |
20090004609 | Lithography process - A lithography process for manufacturing bit-island storage mediums that results in improved resolution and uniformity between bit-islands. The lithography process includes applying a resist coating polymer to a surface of a substrate. Selected areas of the resist coating polymer are then exposed to an energy source, wherein each selected area is exposed to the energy source multiple times to provide a time-averaged exposure of the selected areas that reduces errors caused by noise associated with the energy source. After exposure of the resist coating to the energy source, a selective developer solution is applied to the resist coating to develop the fully exposed regions of the resist coating while leaving undeveloped the partially exposed regions of the resist coating. A polymer reflow material is applied to the developed resist pattern and heated to a selected temperature. The polymer reflow material and selected temperature induce reflow of the developed resist coating such that such that a circumferential diameter associated with the holes formed in the resist pattern is reduced to a desired value distance. The process of inducing reflow of the resist coating can be repeated as desired to achieve a resist pattern wherein the holes formed in the resist pattern are reduced to a desired size. The resist pattern formed on the substrate is then transferred to a magnetic medium to form the desired pattern of bit-islands. | 2009-01-01 |
20090004610 | METHOD AND APPARATUS FOR MANUFACTURING SEMICONDUCTOR DEVICE - A method and apparatus for manufacturing a semiconductor device is disclosed. In particular, the application discloses a method that performs a lithography process using a material capable of increasing a depth of focus so as to prevent efficiency of the lithography process from being degraded due to high integration of a semiconductor device, and a pressure-type bake oven as an apparatus for forming a high refractive material on a semiconductor substrate, having advantages of reducing manufacturing costs of a semiconductor manufacturing process and increasing efficiency of the lithography process. | 2009-01-01 |
20090004611 | LOW VELOCITY STAGED COMBUSTION FOR FURNACE ATMOSPHERE CONTROL - An improved staged combustion method useful with oxy-fuel combustion and in a furnace which contains a charge, wherein substoichiometric combustion and low velocity injection of fuel and primary and secondary oxidant are carried out in an orientation which forms a reducing atmosphere proximate the charge surface. | 2009-01-01 |
20090004612 | Gas-Fired Heating Appliance Having a Flammable Vapor Sensor Control Device - A gas-fired heating appliance includes a burner, a gas valve module for supplying a fuel gas to the burner, a power module for applying a voltage to the gas valve module, and a flammable vapor sensor module for detecting a flammable vapor. The flammable vapor sensor module interrupts the voltage to the gas valve to shut off the gas valve and the burner when the flammable vapor module detects the presence or a predetermined concentration of flammable vapor. The voltage of the power module is applied to the gas valve module through the flammable vapor sensor module. The voltage to the gas valve module is interrupted if the flammable vapor sensor module is short-circuited. This arrangement is tamper-resistant to promote a safe operation of the gas-fired heating appliance. | 2009-01-01 |
20090004613 | Child resistant utility lighter - A child resistant utility lighter having a handle, a barrel mounted in the handle and extending outward, a fuel tank within the handle and for containing liquefied gaseous fuel, a gas valve mounted on the fuel tank, a trigger being able to startup the piezoelectric unit to generate sparks for ignition, and, a lever rotatably mounted on the handle by a lever pin. The utility lighter further has a safe button slidably mounted on the handle and pushed by a first elastic element to an undepressed position; and, a piezoelectric unit contained in the handle and having a starting button, the piezoelectric unit constantly stops at a departure position in which the trigger, when pulled over, is not able to meet the piezoelectric unit, and being able to be pushed to an aligning position by depressing the safe button for ignition by pulling over the trigger. | 2009-01-01 |
20090004614 | CANDLE WITH LID FOR DISPENSING AN AIR TREATMENT CHEMICAL - Candles are provided for dispensing air treatment chemicals in response to heat. There is a lid bearing the air treatment chemical. The candle includes a housing with a combustible fuel. An upper lid includes a cover plate bearing the air treatment chemical and an opening centrally disposed in the plate to allow heated air to pass through when the fuel is being combusted. The plate is suspended across an upper end of a side wall of the housing creating an air gap adjacent the radial periphery of the lid. When the fuel is combusted the air treatment chemical can be heated thereby and carried away by heated air that has passed through the central opening, and the air gap can permit cooler air to enter the cavity while the fuel is being combusted. | 2009-01-01 |
20090004615 | Roller hearth calcining furnace and method of use - A roller hearth calcining furnace that uses a movable fluid bed that is transported through plural heating modules, each heating module independently heated using an indirect heating system. The indirect heating system includes an oxidizer, an all-ceramic, indirect, air-to-air heat exchanger, and one or more metal heat exchangers. The oxidizer can be fired with oil, coal, natural gas or other means, is impervious to attack from dirty fuels, and produces clean hot air for productive use. The movable fluid bed includes a permeable silicon carbide ceramic plate that resides within, and is supported by, a sagger or tray. The sagger is transported on rollers through the plural heating modules, and thus different temperature zones within a furnace as required by the temperature profile of the material being calcined. | 2009-01-01 |
20090004616 | CONSTRUCTIVE DISPOSITION INTRODUCED IN SUPPORTING PIECES FOR ORTHODONTIC ELASTIC RINGS - A constructive disposition introduced in supporting pieces for orthodontic elastics such that the present invention is related with a stem ( | 2009-01-01 |
20090004617 | SELF-LIGATING ORTHODONTIC BRACKETS AND ORTHODONTIC BRACKET SYSTEMS - Self-ligating orthodontic brackets and bracket systems. The orthodontic bracket includes a contact point on its bracket body and a contact point on a movable member used to self-ligate an archwire in an archwire slot defined in the bracket body. The contact points are arranged such that different portions of a tip on an orthodontic hand tool can be placed in a contacting relationship with the contact points and used to place the movable member in an open position. | 2009-01-01 |
20090004618 | ORTHODONTIC HAND TOOLS FOR USE WITH A SELF-LIGATING ORTHODONTIC BRACKET AND METHODS FOR USING SUCH ORTHODONTIC HAND TOOLS - Orthodontic hand tools and methods for using the orthodontic hand tools in conjunction with self-ligating orthodontic brackets. The orthodontic hand tool includes a tip configured to contact a contact point on a bracket body of the bracket and to also contact a contact point on a movable member coupled with the bracket body. When the tip is used, the tip applies a force to the first and second contact points effective to move the movable member relative to the bracket body with negligible force transferred from the tip to the patient's tooth. | 2009-01-01 |
20090004619 | SELF-LIGATING ORTHODONTIC BRACKET AND DEVICES FOR DEPLOYING SAME - An orthodontic bracket for coupling an archwire with a tooth includes a bracket body having an archwire slot adapted to receive the archwire therein and a movable member engaged with the bracket body and movable between an opened position and a closed position. A securing mechanism is configured to secure the movable member in at least the closed position and includes a projecting portion and a receiving portion. The projecting portion may include a generally elongate pin capable of flexing relative to a central axis thereof to secure the movable member in at least the closed position. An orthodontic assembly includes an orthodontic bracket and an alignment device for positioning the bracket on the tooth. | 2009-01-01 |
20090004620 | Surface treating device and surface treating method - A surface treating method for treating a tooth surface and a surface treating device thereof are provided. First, a working gas is filled into a tube. Next, a voltage is provided to the working gas for exciting the working gas into plasma. After that, the plasma is discharged through an opening of the tube for contacting the tooth surface. | 2009-01-01 |
20090004621 | Endodontic Irrigation System - In an endodontic procedure, after the working of a root canal by instruments to remove material and shape the walls of the canal, irrigant is supplied to irrigate and clean the canal. A fluid is applied via a tube which is inserted partway down the root canal and a vacuum is supplied via the cannula. Alternately, the fluid may be supplied through the cannula and vacuum through the tube. The tube and cannula may or may not pass through a material created by a standard dental filling material of a composite nature which provides a seal at a position near the top of the coronal opening. The cleaning may be performed using a cannula having a slight taper with one or a series of larger holes near the top and smaller holes near the bottom; or a pair of cannulas of substantially the same diameter and taper, one with one or a series of larger holes near the top, while the other one with one or a series of holes near the bottom of the cannula. | 2009-01-01 |
20090004622 | Dental, Dental-Medical or Dental-Technical Handpiece With Electric Motor - In a dental, dental-medical or dental-technical handpiece, having an elongate grip sleeve there are provided at the rearward end of the grip sleeve coupling elements for the connection of the handpiece to a supply hose to be connected with a dental supply arrangement, and there are provided at the forward end of the grip sleeve a head section with a tool receiver for mounting a tool to be put into rotation. The handpiece further has an electric motor for driving a tool held by the tool receiver, which motor is completely integrated into the grip sleeve such that grip sleeve and motor form a unit. | 2009-01-01 |
20090004623 | SUCKBACK PREVENTION DEVICE FOR SINGLE USE HIGH-SPEED TURBINE DENTAL DRILL HANDPIECE - A suckback prevention device for single use high-speed turbine dental drill handpiece, installed at the side assembled with the drilling needle of the head housing of the high-speed turbine dental drill handpiece which comprises a head, a drilling needle ( | 2009-01-01 |
20090004624 | Piezo Insert and Piezo Packer for Operating an Implant Surgical Operation Using Piezoelectric Device - The invention concerns a piezo insert and a piezo packer for implant surgical operation with a piezoelectric device. The piezo insert according to the invention consists of a piezo insert body one end of which is inserted in and fixed to a hand piece of the piezoelectric main device; an insert pole extending from the other end of the piezo insert body; and an insert tip formed at the end of the insert pole. The piezo packer consists of a piezo packer body one end of which is inserted in and fixed to a hand piece of a piezoelectric main device; a packer pole extending from the other end of the piezo packer body and a packer tip formed at the end of the packer pole. With the piezo insert and the piezo packer according to the invention, forming and expanding a hole in a vertical direction in a residual ridge at a portion for implant placement is facilitated when applying bone grafting in a maxillary sinus, and damages can be avoided in a maxillary sinus membrane, in order to effectively insert graft materials through the formed hole in the residual ridge for bone grafting. | 2009-01-01 |
20090004625 | Dental Drilling Assembly - A dental drilling assembly comprises a drill ( | 2009-01-01 |
20090004626 | Multi-Cortial Dental Implant Anchor, Dental Implant Kit and Template - A multi-cortical implant anchor for use with a dental implant having a generally elongate shank and a grippable region formed at a predetermined portion thereof, the implant anchor comprises a first elongate member and a second elongate member connected therebetween via a bridge member to generally form a member having a longitudinal axis, the first elongate member and the second elongate member being adapted to lockably engage the grippable region of the dental implant against pivoting in a direction transverse to the longitudinal axis. | 2009-01-01 |
20090004627 | Dental Material And Composite Dental Material Formed By Using Hydroxy Apatite - It is intended to provide a dental material formed by using a hydroxy apatite, which is suitable for performing the guided bone regeneration (GBR) method in the implant therapy of dentistry. Also, it is intended to provide a composite dental material which is a bioabsorbable film provided integrally with hydroxy apatite, which is capable of rapidly attracting the osteoblast and blocking the fibroblast. The dental material is a membrane to be used for a guided bone regeneration method of guiding a bone formation by preventing invasion by anaplastic fibroblast by covering a bone developing site with a blocking membrane and filling the bone developing site with osteoblast, wherein a membrane main body of the blocking membrane is formed by using hydroxy apatite having osteoconductive capability of rapidly attracting osteoblast for the purpose of disposing the hydroxy apatite at a bone developing site side of the membrane main body. The composite dental material is a membrane to be used for a guided bone regeneration method of guiding a bone formation by preventing invasion by anaplastic fibroblast by covering a bone developing site with a blocking membrane and filling the bone developing site with osteoblast, wherein a membrane main body of the blocking membrane is formed by using a bioabsorbable membrane, and the bioabsorbable membrane is integrally provided with hydroxy apatite having osteoconductive capability of rapidly attracting osteoblast and a structure of being disposed at a bone developing site side of the membrane main body. | 2009-01-01 |
20090004628 | Tissue desensitizing system and method - A method and device for controlling the pain of a periodontal ligament injection, and other minor medical and dental procedures. The device desensitizes the tissues utilizing cold, vibration, or both. The method is useful for injections, small biopsies, intraosseous drilling, blood sampling, and so on. | 2009-01-01 |
20090004629 | AESTHETIC DENTAL ARCH LAMINATES AND ADHESIVE - The invention relates to a dental kit and method for comfortably improving oral aesthetics, including one or more thin aesthetic dental arch laminates and a non-toxic tackification adhesive. The one or more thin aesthetic dental arch laminates is configured for comfortable, easy, reusable application, adhered to a person's teeth by the non-toxic tackification adhesive, which is not a bonding agent or a cement. The one or more thin aesthetic dental arch laminates can accommodate the person's teeth without tensioning the person's teeth or the one or more thin aesthetic dental arch laminates, enhancing comfort and minimizing breakage. | 2009-01-01 |
20090004630 | Strengthened ceramic restoration - The present invention relates to a process for the preparation of a full-ceramic dental restoration, comprising heat pressing of a tooth coloured pressing glass on a fully or partially supporting structure of yttria doped tetragonal zirconia (YTZP), comprised of yttria doped tetragonal zirconia ceramic having a grain size, as measured by the linear intercept method, of less than 0.6 μm, wherein the pressing glass has a thermal expansion coefficient (TEC) of between 9.0 and 11.0 μm/m.K (measured in the range of from 25 to 500° C.) and wherein the pressing glass has a pressing temperature of between 750 and 1200° C. In a further aspect, the invention relates to a full-ceramic dental restoration, comprising a fully or partially supporting structure of yttria doped tetragonal zirconia (YTZP) and a heat pressed tooth coloured pressing glass, which restoration is modeled to be in occlusal contact with opposing teeth and in mesio-distal contact with neighbouring teeth. | 2009-01-01 |
20090004631 | Dental examination and treatment tool - The invention relates to a Dental examination or treatment tool. the tool comprises at least one lumen in which at least one imaging catheter is or can be accommodated for recording image data of an examination or treatment area. | 2009-01-01 |
20090004632 | Braille Writing Apparatus With Information Processing Function - A Braille writing device capable of processing information, comprises a motherboard ( | 2009-01-01 |
20090004633 | INTERACTIVE LANGUAGE PRONUNCIATION TEACHING - Techniques for language instruction and teaching are described. Methods focus on the sound distinctions that learners have trouble discriminating. Learners practice discriminating these sounds. A learning system is developed using databases of speech from people discriminating these sounds. An embodiment of a method according to the present disclosure can utilize sets of words that differ by only a single syllable containing a sound that is difficult to pronounce, as a way to teach the pronunciation of a word. The sets of similar words can be of a desired number or have a desired number of constituent members. Embodiments of systems can include user interfaces and a automated speech recognition system, including suitable automated speech recognition software, that can interact with a user, e.g., in a pedagogical setting. Related software products including computer-readable instructions resident in a computer-readable medium are described. HMM and DTW algorithms may be used for the embodiments. | 2009-01-01 |
20090004634 | System and Method for Preventing and Treating Repetitive Stress Injury - A system and method for is disclosed that plays a video of a graphical representation of a person demonstrating a selected physical exercise that is aimed at preventing or treating a repetitive stress injury in one or more areas of the body. The video demonstrates the exercise in real time so that a user performing the exercise, in time with the demonstration, will perform the exercise in a proper period of time that increases the benefits of the exercise. The selected exercise could be either directly selected from a list of exercises, from a reduced list of exercises that focus or effect a specific body area chosen by the user, or one of a set of exercises. Alternatively, a scheduling event could be used to display a demonstration of a selected exercise upon the occurrence of a predetermined event. | 2009-01-01 |
20090004635 | Methods for multi-sensory virtual golf instruction - A method and devices are disclosed for instructing students in a physical skill and allowing students to experience specific physical movements involved in a sport or physical skill. The method, program and system comprise instructions, stories, structured activities, games, mechanical figures, and devices which synergistically engage the student's kinesthetic learning facilities. Stimulation of these learning facilities is achieved by engagement in multi-sensory interaction, simulation of physical movements, demonstration of the underlying physics of the movements, interaction with structured activities and games. Educational devices using process-oriented methods are disclosed to engage multiple learning styles in the process of instruction of a sport or physical skill. These devices may include visual, manipulative, and computer-adapted models designed to allow visual, auditory, kinesthetic learners to learn and experience the sport or physical movement. Further, this invention allows motion-impaired individuals a unique opportunity to experience a sport at a kinesthetic level. | 2009-01-01 |
20090004636 | MODEL HUMAN EYE - A model human eye is disclosed. The model human eye comprises a extended hemispherical-shaped assembly comprising a center point, an open end defined by a first continuous distal end, a cornea portion symmetrically disposed around the center point, and a sclera portion disposed between the cornea portion and the first continuous distal end. The extended hemispherical-shaped assembly comprises an integral molding. The model human eye further comprises a lenticular bag disposed within the extended hemispherical-shaped assembly. The model human eye further comprises a base comprising a bottom and one or more walls attached to that bottom and extending outwardly therefrom, where the one or more walls comprise a second continuous distal end. The first continuous distal end is attached to the second continuous distal end to define an enclosed space. The lenticular bag separates the enclosed space into an anterior chamber and a posterior chamber. | 2009-01-01 |
20090004637 | MODEL HUMAN EYE - A model human eye comprising a cylindrical base portion comprising a bowl-shaped substrate disposed therein, and a plurality of retinal layers stacked on the surface of that bowl-shaped substrate. The model eye further comprises a hemi-spherical-shaped top portion comprising a visually transparent cornea portion and a visually opaque sclera portion comprising an inner surface, an annular iris continuously attached to the inner surface and extending inwardly therefrom, and a lenticular bag continuously attached to the iris, wherein the iris in combination with the lenticular bag and the cornea portion define an anterior chamber. The top portion is attached to the base portion to define a posterior chamber. | 2009-01-01 |
20090004638 | LATENT PROPERTY DIAGNOSING PROCEDURE - The present invention provides a method of doing cognitive diagnosis of mental skills, medical and psychiatric diagnosis of diseases and disorders, and in general the diagnosing of latent properties of a set of objects, usually people, for which multiple pieces of binary (dichotomous) information about the objects are available, for example testing examinees using right/wrong scored test questions. Settings where the present invention can be applied but are not limited to include classrooms at all levels, web-based instruction, corporate in-house training, large scale standardized tests, and medical and psychiatric settings. Uses include but are not limited to individual learner feedback, learner remediation, group level educational assessment, and medical and psychiatric treatment. | 2009-01-01 |
20090004639 | INTERACTIVE TEACHING AID - A teaching aid is provided. The teaching aid can include a first portion. The first portion can include at least one shape, at least one fine motor skill activity and at least one alphabet. The teaching aid can also include a second portion. The second portion can include a numeric scale, at least one self esteem activity, at least one safety activity and at least one large motor skill activity. The first portion can be coupled to the second portion to form an aid to teach early childhood development skills for preschool. | 2009-01-01 |
20090004640 | Method of Paraffin-Penetration Treatment for Tissue Preparation and Apparatus for the Treatment - The present invention provides a paraffin-penetration treatment method for a tissue preparation, which is capable of enabling sufficient penetration of paraffin even into a tissue preparation, into which paraffin can hardly penetrate by a conventional paraffin-penetration treatment method. When the penetration of the paraffin into the tissue preparation is performed by immersing the tissue preparation in molten paraffin after a dehydrating and a degreasing treatments, the method comprises the steps of: immersing the tissue preparation in the molten paraffin so as to allow the paraffin to penetrate into the tissue preparation; cooling the tissue preparation so as to solidify the paraffin having penetrated thereinto; and immersing the tissue preparation in the molten paraffin again so as to melt the paraffin having penetrated thereinto, and the above described solidification treatment and melting treatment are conducted at least once for one tissue preparation. | 2009-01-01 |
20090004641 | SITE-SPECIFIC LABELING OF AFFINITY PEPTIDES IN FUSION PROTEINS - The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment. | 2009-01-01 |
20090004642 | In vitro Method for the Determination of Glycemic Index of Food Products - The present invention provides an in vitro method for determining the glycemic index values for various food products. The present invention provides an accurate and inexpensive in vitro method for determining the glycemic index of a wide variety of both food ingredients and finished food products. | 2009-01-01 |
20090004643 | METHODS FOR CONCURRENT IDENTIFICATION AND QUANTIFICATION OF AN UNKNOWN BIOAGENT - The present invention provides methods for the quantification of an unknown bioagent in a sample by amplification of nucleic acid of the bioagent, and concurrent amplification of a known quantity of a calibration polynucleotide from which are obtained a bioagent identifying amplicon and a calibration amplicon. Upon molecular mass analysis, mass and abundance data are obtained. The identity of the bioagent is then determined from the molecular mass of the bioagent identifying amplicon and the quantity of the identified bioagent in the sample is determined from the abundance data of the bioagent identifying amplicon and the abundance data of the calibration amplicon. | 2009-01-01 |
20090004644 | METHODS AND COMPOSITIONS FOR PROCESSES OF RAPID SELECTION AND PRODUCTION OF NUCLEIC ACID APTAMERS - Embodiments herein relate to compositions and methods for making and using aptamers, for example, DNA aptamers (DCEs) and/or RNA aptamers. In some embodiments, methods relate to making and amplifying target DCEs. In certain embodiments, methods for making capture elements or aptamers concern using a reporter moiety and signal reducing moiety prior to amplifying a target-specific capture element. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of aptamers such as DCEs directed to a particular target agent. Some embodiments relate to systems for performing automated generation of aptamers. | 2009-01-01 |
20090004645 | Methods for Identifying Genes that Mediate a Response of a Living Cell to an Agent - In one aspect, the present invention provides methods for determining whether a gene mediates the response of a living cell to an agent. In another aspect, the present invention provides methods to identify a mammalian subject responsive to a KSP inhibitor. | 2009-01-01 |
20090004646 | Method for Quantifying Methylated Dna - The inventive method makes it possible to quantify methylated DNA by combining the restricted digestion and real-time PCR. For this purpose, the inventive method consists in isolating the examined DNA from a biological sample, in reacting the isolated AND with a methylation specific restriction enzyme, in amplifying by means of a real-time PCR, wherein the amplified products are formed only when the DNA is precut-off and, afterwards, in calculated the methylated and unmethylated DNA proportion in the initial sample with the aid of a reference measurement. Said method is particularly suitable for diagnosis and prognosis cancer and other diseases associated with a methylation state modification and for predicting the drug effects. | 2009-01-01 |
20090004647 | Method of Judging Grade of Malignancy of Carcinoma Cell - Means for easily determining the grade of malignancy of cancer cells. The amount of ATBF1 in the whole cell structure of test cancer cells separated from a living organism is detected, and on the basis of detection results, the grade of malignancy of test cancer cells is judged. Alternatively, the amount of ATBF1 in the nuclei of test cancer cells separated from a living organism is detected, and on the basis of detection results, the grade of malignancy of test cancer cells is judged. Still alternatively, the amount of ATBF1 in the cytoplasms of test cancer cells separated from a living organism is detected, and on the basis of detection results, the grade of malignancy of test cancer cells is judged. In a preferred from, at least one of (1) the amount of intranuclear presence and/or intracytoplasmic presence a region corresponding to exon 10 of an ATBF1 gene, (2) the amount of intranuclear presence and/or intracytoplasmic presence a region corresponding to exon 11 of an ATBF1 gene, and (3) the amount of intranuclear presence and/or intracytoplasmic presence a region corresponding to exon 3 of an ATBF1 gene is detected as the amount of ATBF1. | 2009-01-01 |
20090004648 | Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis of ovarian cancer - Novel markers for ovarian cancer that are both sensitive and accurate. These markers are overexpressed and/or differentially expressed in ovarian cancer specifically, as opposed to normal ovarian tissue. The measurement of these markers, alone or in combination, in patient samples provides information that the diagnostician can correlate with a probable diagnosis, in ovarian cancer. The markers of the present invention, alone or in combination, show a high degree of differential detection between ovarian cancer and non-cancerous states. | 2009-01-01 |
20090004649 | EVERNINOMICIN BIOSYNTHETIC GENES - This invention is directed to nucleic acids which encode the proteins that direct the synthesis of the orthosomycin everninomicin and to use of the nucleic acids and proteins to produce compounds exhibiting antibiotic activity based on the everninomycin structure. The DNA sequence for the gene clusters responsible for encoding everninomicin biosynthetic genes, which provide the machinery for producing everninomicin, are provided. Thus, this invention provides the nucleic acid sequences needed to synthesize novel everninomicin-related compounds based on everninomicin, arising from modifications of the DNA sequence designed to change glycosyl and modified orsellinic acid groups contained in everninomicin. A | 2009-01-01 |
20090004650 | Methods for detecting a Cyclophilin B SNP associated with Herda - This invention provides compositions and methods for identification of carriers of Hereditary Equine Regional Dermal Asthenia (HERDA) in equine species. In particular, this invention identifies a single nucleotide polymorphorism (SNP) in cyclophlin B that can be used to identify carriers of HERDA and individuals affected by HERDA. | 2009-01-01 |
20090004651 | Screening methods for compounds that modulate the activity of G-protein coupled receptors - The present invention relates to a screening system for modulators of GPCRs. Further it relates to recombinant vector systems for the heterologous expression of heterodimeric g-protein coupled receptors (GPCRs) in eucaryotic host cells. Preferably the functional expression of engineered GPCRs for the perception of sweet and L-amino acid taste or more preferably the use of said receptors for the identification of functional ligands is also encompassed. | 2009-01-01 |
20090004652 | Methods for identifying and using SNP panels - Described are methods for identifying single nucleotide polymorphism (SNPs) that are useful for analyzing genetic samples, and for using said SNPs to determine genetic identity of samples. | 2009-01-01 |
20090004653 | Sulfonated [8,9]benzophenoxazine dyes and the use of their labelled conjugates - Fluorescent, sulfonated 3,7-diamino-[8,9]benzophenoxazine dyes are provided that are especially useful for labelling biopolymers and other substrates. The dye-labelled conjugates can be used in a variety of contexts, including cell surface assays employing intact, live cells and in nucleic acid detection methods. The new dyes are water soluble and can be conjugated to a variety of substrates, such as polynucleotides, nucleosides, nucleotides, peptides, proteins, antibodies, carbohydrates, ligands, particles and surfaces. | 2009-01-01 |
20090004654 | METHOD FOR THE DETECTION OF BACTERIAL SPECIES OF THE GENERA ANAPLASMA/EHRLICHIA AND BARTONELLA - The present invention relates to a method for the detection and identification of bacterial species belonging to the genera | 2009-01-01 |
20090004655 | RNA BIOASSAY - The present invention relates to methods for evaluating the cell damaging potential of an agent by determining the ability of the agent to increase messenger RNA release in cells. | 2009-01-01 |
20090004656 | THERMOSTABLE POLYMERASES HAVING ALTERED FIDELITY AND METHODS OF IDENTIFYING AND USING SAME - The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant. | 2009-01-01 |
20090004657 | 7B2 Knockout transgenic animals as models of endocrine disease - In general, the invention features methods and uses for transposon-mediated gene targeting which greatly enhance the insertion and detection of desired genes in genomic exons by homologous recombination. The invention also features transgenic non-human mammals, and eukaryotic cells, wherein a gene encoding 7B2 protein is modified, as well as nucleic acid vectors capable of undergoing homologous recombination with an endogenous 7B2 gene in a cell. The invention also features transgenic non-human mammals as models of endocrine disorders, as well as methods for diagnosing and treating patients with endocrine disorders. | 2009-01-01 |
20090004658 | INTEGRIN ALPHA 7 MUTATIONS IN PROSTATE CANCER, LIVER CANCER, GLIOBLASTOMA MULTIFORME, AND LEIOMYOSARCOMA - Methods are provided for determining the presence of a cancer in a biological sample, such as a tissue biopsy. The methods comprise determining if integrin alpha 7 expression is decreased in the biopsy which is indicative of the presence of a cancer or likelihood of relapse of a cancer. This can be accomplished by determining if levels of integrin alpha 7 mRNA or protein are decreased as compared to a control. This also can be accomplished by determining if a mutation in the integrin alpha 7 gene is present in the biopsy. | 2009-01-01 |
20090004659 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 4 and mutated sequences thereof. | 2009-01-01 |
20090004660 | METHOD FOR DETECTING NEUROBLASTOMA AND ITS MALIGNANCY AND METHOD FOR SUPPRESSING THE SAME - An object of the present invention is to provide a method for detecting cancer and a cell growth inhibitor through identification of genes exhibiting characteristic behavior in the cases of cancer such as neuroblastoma. The present invention provides a method for detecting cancer, which comprises detecting canceration including malignancy of a specimen through detection of inactivation of a gene in the q22 region of chromosome 14 in the specimen. | 2009-01-01 |
20090004661 | METHOD OF GROWING MESENCHYMAL STEM CELLS FROM BONE MARROW - The present invention provides a method for culturing mesenchymal stem cells using cord blood serum, for therapeutic purposes in regenerative medicine; and in particular the present invention provides for the use of these cells in the treatment of PD, and the present invention has provided proliferation and neuronal differentiation of the MSCs in a xenofree culture system for clinical applications in a simple two step protocol, and the in vivo functional efficacy was tested in Parkinson's animal model. | 2009-01-01 |
20090004662 | METHOD AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION - The present teachings provide methods, compositions, and kits for nucleic acid amplification. In some embodiments of the present teachings, amplification reactions are performed with at least one high stability primer. In some embodiments, the present teachings provide a method comprising a high stability primer for amplification of a nucleic acid sequence in a sample comprising a target nucleic acid sequence and a PCR inhibitor. | 2009-01-01 |
20090004663 | Real-Time Assays Of Neuro-Humoral Factors To Assess Cardiovascular Stress - The present invention relates to rapid assays for neuro-humoral factors modulated in response to cardiovascular stress and integration of data obtained from such assays to provide profiles of response to cardiovascular stress that can guide therapy. | 2009-01-01 |
20090004664 | HCV NS3 replicon shuttle vectors - The present invention provides for novel HCV NS3 replicon shuttle vectors useful for cloning in HCV polynucleotide sequences from samples of HCV-infected patients and testing the resulting replicons for drug susceptibility. | 2009-01-01 |
20090004665 | METHODS AND COMPOSITIONS FOR ISOLATING NUCLEIC ACID SEQUENCE VARIANTS - The invention is drawn to isolating sequence variants of a genetic locus of interest using a modified iterative primer extension method. The nucleic acids analyzed are generally single stranded and have a reference sequence which is used as a basis for performing iterative single nucleotide extension reactions from a hybridized polymerization primer. The iterative polymerization reactions are configured such that polymerization of the strand will continue if the sequence of the nucleic acid being analyzed matches the reference sequence, whereas polymerization will be terminated if the nucleic acid being analyzed does not match the reference sequence. Nucleic acid strands that have mutations can be isolated using a variety of methods and sequenced to determine the precise identity of the mutation/polymorphism. By performing the method on both strands of the nucleic acid being analyzed, virtually all possible mutations can be identified. | 2009-01-01 |
20090004666 | METHOD OF DETECTING NUCLEOTIDE SEQUENCE WITH AN INTRAMOLECULAR PROBE - A nucleotide sequence-detecting method, including preparing a first intramolecular detecting sequence having a sequence complementary to a first sequence located at a 3′-side of the detecting site contained in the nucleotide sample and a second intramolecular detecting sequence having a sequence complementary to a second sequence located at a 5′-side of the detecting site, preparing a detecting chain containing a sequence of the detecting chain by connecting the first intramolecular detecting sequence to the 3′ terminal of the nucleotide sample and the second intramolecular detecting sequence to the 5′ terminal, allowing intramolecular hybridization at two positions of the detecting chain, connecting the 3′ terminal of the first intramolecular detecting sequence to the 5′ terminal of the second intramolecular detecting sequence, obtaining a cyclic structure, detecting the desired sequence in the nucleotide sample from the cyclic structure. | 2009-01-01 |
20090004667 | METHOD FOR GENERATING APTAMERS WITH IMPROVED OFF-RATES - The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently crosslinking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element. | 2009-01-01 |
20090004668 | Pre-miRNA loop-modulated target regulation - By employing essential nucleotides from both the stem and loop of precursor-miRNA, greater specificity is achieved as to the mRNAs that are repressed. It is found that besides the seed sequence of the stem of the pre-miRNA, nucleotides in the loop affect the activity and specificity of the cursor- and the processing and binding to target mRNA. By using both sequences in the natural pre-miRNA or modified mimetics, one can screen for cellular miRNA expression, modulate cell properties with greater specificity and investigate cellular activity as to phenotype and response to external stimuli in the presence and absence of target protein expression. | 2009-01-01 |
20090004669 | Methods of diagnosing pre-eclampsia or eclampsia - Disclosed herein are methods for diagnosing pre-eclampsia and eclampsia. Also disclosed herein are methods for treating pre-eclampsia and eclampsia using compounds that increase VEGF or PlGF levels or compounds that decrease sFlt-1 levels. Compounds that inhibit the binding of VEGF or PlGF to sFlt1- are also disclosed herein for the treatment of pre-eclampsia or eclampsia. | 2009-01-01 |
20090004670 | METHODS FOR FABRICATING SURFACE ENHANCED FLUORESCENT (SEF) NANOPARTICLES AND THEIR APPLICATIONS IN BIOASSAYS - Embodiments of the invention relate to SEF nanoparticles with increased fluorescence, methods of making SEF nanoparticles, and their application in various bioassays for the detection of target bioanalytes. One embodiment includes the SEF nanoparticle itself, a second embodiment includes the fabrication of SEF nanoparticles, a third embodiment includes methods of using SEF nanoparticles in biodetection assays. A final embodiment includes kits to be used in the fabrication of SEF nanoparticles. | 2009-01-01 |
20090004671 | Marker Protein For Use In Diagnosis Of Pancreatic Cancer - Disclosed herein are a pancreatic carcinoma marker protein that is a blood protein having a molecular weight of 28,080±15, 17,272±9, 17,253±9, 8,766±5, or 14,779±8 (m/z) and a method for detecting pancreatic carcinoma using the marker protein as an indicator. | 2009-01-01 |
20090004672 | 17867, A NOVEL HUMAN AMINOPEPTIDASE - The present invention relates to a newly identified human aminopeptidase. The invention also relates to polynucleotides encoding the aminopeptidase. The invention further relates to methods using the aminopeptidase polypeptides and polynucleotides as a target for diagnosis and treatment in aminopeptidase-related disorders. The invention further relates to drug-screening methods using the aminopeptidase polypeptides and polynucleotides to identify agonists and antagonists for diagnosis and treatment. The invention further encompasses agonists and antagonists based on the aminopeptidase polypeptides and polynucleotides. The invention further relates to procedures for producing the aminopeptidase polypeptides and polynucleotides. | 2009-01-01 |
20090004673 | Method for Determining Condition of Disseminated Intravascular Coagulation - A method for determining a condition of disseminated intravascular coagulation (DIC), by analyzing the amount and/or enzyme activity of a von Willebrand factor (vWF)-cleaving protease (ADAMTS13) (preferably also the amount of vWF) in a patient suffering from DIC, and a kit for determining a condition of DIC, comprising an antibody or a fragment thereof which specifically binds to ADAMTS13, are disclosed. According to the present invention, a differential diagnosis of patients with thrombotic thrombocytopenic purpura (TTP) can be carried out from among patients with DIC, which could not be distinguished on the basis of only clinical findings or known markers. | 2009-01-01 |
20090004674 | ACPL-related assays - The invention is directed to purified and isolated novel ACPL polypeptides, the nucleic acids encoding such polypeptides, processes for production of recombinant forms of such polypeptides, antibodies generated against these polypeptides, fragmented peptides derived from these polypeptides, and the uses of the above. | 2009-01-01 |
20090004675 | Method of Identifying Compounds for Bacterial Growth Modulation - The present invention relates to a method of identifying a candidate compound for modulating bacterial growth. This the method involves providing a β clamp peptide from a bacterial replicase, providing a second peptide that binds to at least one amino acid of SEQ ID NO:9 that is not designated X, wherein the second peptide does not exhibit polymerase activity, and providing a test compound. The β clamp peptide and the second peptide are contacted with the test compound, and the level of binding between the β clamp peptide and the second peptide in the presence of the test compound is determined. The level of binding between the β clamp peptide and the second peptide in the presence of the test compound is then compared to a control that does not contain the test compound. A test compound that alters the level of binding between the β clamp peptide and the second peptide compared to the control is a candidate compound for modulating bacterial growth. | 2009-01-01 |
20090004676 | T1R3 receptor binding assays for identification of taste modulatory compounds - Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically. T | 2009-01-01 |
20090004677 | QUANTITATIVE DETERMINATION OF BCL10 - Methods of and kits for quantitatively determining the concentrations of Bcl10 in a biological sample were developed that provide an accurate means of identifying therapeutic molecules with a plurality of therapeutic effects. A solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) for human Bcl10 that provides reproducible, precise measurements was developed and characterized. The sensitivity of the assay is 0.25 ng/ml, enabling accurate detection of small quantities of Bcl10. The sensitive and specific, solid-phase, sandwich ELISA for Bcl10 is well-suited for the accurate determination of Bcl10 values in different experimental conditions, in immune and non-immune cells, and may find use in a clinical context. An ELISA as described herein may have particular clinical utility, since increased Bcl10 is associated with inflammation, infection and malignancy. | 2009-01-01 |
20090004678 | METHOD FOR SCREENING FERTILITY AND NEW COMPOUNDS OR MOLECULES, USING CRT OR ERP57 TRANSLOCATION - A screening method for testing the immunogenicity of new molecules and compounds and for testing fertility and sterility conditions. The method includes inducing a translocation of a calreticulin protein and/or an ERP57 protein, to a cellular membrane in order to determine the immunogenicity of the new molecules and compounds, or the fertility or sterility conditions. The method includes also the using of recombinant CRT and/or ERP57 to treat sterility. | 2009-01-01 |
20090004679 | METHOD FOR DETERMINING PLASMINOGEN ACTIVATOR INHIBITOR - A method is provided for determining active plasminogen activator inhibitor—Type 1 (PAI-1) in a biological fluid, the method comprising the steps of (i) providing a sample of a biological fluid; and (ii) measuring the amount of PAI-1/multimeric vitronectin complex in the sample to determine active PAI-1 in the sample. A kit for carrying out the method is also provided. | 2009-01-01 |
20090004680 | DETERMINING AND REDUCING IMMUNORESISTANCE TO BOTULINUM TOXIN THERAPY USING BOTULINUM TOXIN A PEPTIDES - The present invention provides BoNT/A peptide compositions, tolerogizing compositions, BoNT/A immune response inducing compositions and antibody compositions, as well as methods of determining immunoresistance to botulinum toxin therapy in an individual, methods of preventing or reducing immunoresistance to botulinum toxin therapy in an individual, methods of vaccinating an individual against botulinum toxin, methods of preparing anti-BoNT/A antibodies, methods of treating botulinum toxicity in an individual and methods of reducing anti-botulinum toxin antibodies in an individual. | 2009-01-01 |
20090004681 | PLATELET AGGREGATION MEASURING METHOD AND PLATELET AGGREGATION MEASURING APPARATUS - A platelet aggregation measuring method comprising: preparing a measurement sample which contains a sample and a reagent which includes a platelet activator; mixing the measurement sample at a first speed; mixing the measurement sample at a second speed which is greater than the first speed after mixing the sample at the first speed; obtaining optical information from the measurement sample while mixing the measurement sample at the second speed; and analyzing aggregation of platelets in the sample based on the optical information is disclosed. A platelet aggregation measuring apparatus is also disclosed. | 2009-01-01 |
20090004682 | Method for Measuring Glucose Concentration in Blood Using Infrared Spectroscopy and Instrument Employing It - The objective of the present invention is to provide a method and instrument for measuring glucose concentration in blood using infrared spectroscopy. Glucose concentration in blood is measured based on an integrated value obtained by measuring an absorption spectrum that includes the wavelength range of 1020-1040 cm | 2009-01-01 |
20090004683 | Indicator Agent for Noninflammatory Stress Response and Use Thereof - A system enabling the molecular biological visualization and quantitative detection of events in a stress-exposed living organism and a means enabling the management of stress are provided. An indicator agent for non-inflammatory stress responses mediated by superoxide, which comprises IL-18, a visualizing agent for non-inflammatory stress responses for detecting the aforementioned indicator agent, a method of measuring the degree of non-inflammatory stress, which comprises using the aforementioned visualizing agent, a method of preventing, ameliorating or predicting a change in immune status based on a non-inflammatory stress response, which comprises applying the aforementioned visualizing agent to an animal, and a therapeutic agent for a change in immune status based on a non-inflammatory stress response for reducing the amount or activity of the aforementioned indicator agent. | 2009-01-01 |
20090004684 | Compositions and Methods for Detection and Quantification of Protein Oxidation - Compositions and methods for detecting carbonyl functional groups on polypeptides are disclosed. | 2009-01-01 |
20090004685 | Interface Device and Method for Using the Same - The present disclosure is related to an interface device for providing access to a network to be monitored. The interface device includes a plurality of elements, the elements being sensors and/or actuators. A selection means is provided for selecting a subset of elements among the plurality of elements, each element of the subset being arranged for outputting and/or receiving a signal. A local memory is provided for storing the subset. | 2009-01-01 |
20090004686 | Measurement and uses of oxidative stress - The present invention provides a method of determining the overall oxidative status of a body fluid or a tissue of a patient by measuring the oxidation-reduction potential (ORP) of the body fluid or tissue. The method has been found to be useful in the diagnosis, evaluation and monitoring of patients who have suffered a trauma (such as a head injury), patients suspected of being critically-ill, patients who have a viral infection, and patients suspected of having a myocardial infarction. The method has also been found useful in monitoring and evaluating exercise performance in patients. In addition, the method has been found useful in monitoring and evaluating stored blood products and patients who will receive such a product. | 2009-01-01 |
20090004687 | PREDICTIVE MARKERS FOR OVARIAN CANCER - Methods are provided for predicting the presence, subtype and stage of ovarian cancer, as well as for assessing the therapeutic efficacy of a cancer treatment and determining whether a subject potentially is developing cancer. Associated test kits, computer and analytical systems as well as software and diagnostic models are also provided. | 2009-01-01 |
20090004688 | Sampling System and Method - A sampling system and method may use a kit that has a package enclosing a swab and a receptacle. The swab may have a shaft and a tip. The tip may be substantially made of calcium alginate. The receptacle may contain a diluent, such as sodium citrate, and glass beads. The swab may be used in an environment to sample microorganisms present on surfaces or in equipment. The kit may be used in a method of sampling and the resulting sample may be used in a method of quantitative testing. | 2009-01-01 |
20090004689 | Lineage Restricted Glial Precursors from the Central Nervous System - A glial precursor cell population from mammalian central nervous system has been isolated. These A2B5 | 2009-01-01 |
20090004690 | COMPOSITION AND METHODS FOR MAKING 5, 10, 15, 20-TETRAKIS (CARBOXYPHENYL) PORPHINE - Presented is a method to detect precancerous states in mammalian cell and tissue samples comprising incubating a sample with solubilized 5, 10, 15, 20-tetrakis (carboxyphenyl)porphine (TCPP), measuring TCPP fluorescence in the sample, and categorizing the sample as non-cancerous, precancerous or cancerous based on TCPP fluorescence, as correlated with the respective states of the cells. In conjunction with the method a detection, a novel and more efficient method of solubilizing TCPP is presented, as well as a composition comprising TCPP solubilized by this method. | 2009-01-01 |
20090004691 | Enhanced Fluidic Method and Apparatus for Automated Rapid Immunohistochemistry - A sample processing system that may be configured to achieve rapid sample processing such as rapid histochemical processing may involve a wave element that can use angular microscopic slide movements to cause repeated elimination and reapplication of a fluidic substance perhaps through the action of capillary motion in order to refresh a microenvironment adjacent to a sample such as a biopsy or other such sample. Through such refreshing of a microenvironment, depletion of the microenvironment is avoided and the time necessary for slide processing may be dramatically shortened from a more common 60 to 120 minute to perhaps less than 15 minutes so as to permit use of such a system in an intraoperative or surgical environment such as recommended by the College of American Pathologists intraoperative guidelines or the like. Patients may thus avoid a need to be subjected to an additional surgical procedure when lab results become available to see if tumors or the like were fully removed in a prior procedure. | 2009-01-01 |
20090004692 | METHODS TO ENHANCE THE ACTIVITY OF LIGNOCELLULOSE-DEGRADING ENZYMES - Methods for hydrolyzing lignocellulose are provided, comprising contacting the lignocellulose with at least one chemical treatment. Methods for pretreating a lignocellulosic material comprising contacting the material with at least one chemical are also provided. Methods for liberating a substance such as an enzyme, a pharmaceutical, or a nutraceutical from plant material are also provided. These methods are more efficient, more economical, and less toxic than current methods. | 2009-01-01 |
20090004693 | Production of Polypeptides by Improved Secretion - The present invention relates to polypeptides that have an activity corresponding to at least one activity of the SEC61 polypeptide, polynucleotides encoding these polypeptides and the use thereof in the preparation of host cells suitable for production of a polypeptide of interest. Such host cells may have an increased capacity to secrete a polypeptide of interest. | 2009-01-01 |
20090004694 | Sequence of Thermotolerant L-Rhamnose Isomerase Gene and Use of the Same - [PROBLEMS] To provide the sequence of a thermotolerant L-rhamnose isomerase gene. [MEANS FOR SOLVING PROBLEMS] A DNA comprising the base sequence represented by SEQ ID NO:1. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A protein originating in | 2009-01-01 |
20090004695 | Process for constructing strain having compactin hydroxylation ability - The present invention is directed to methods and compositions for microbial based production of pravastatin. The compositions of the invention include novel strains of microorganisms that are capable of efficiently hydroxylating compactin (ML-236 B) resulting in production of pravastatin. In particular, the microorganisms of the invention are genetically engineered to express both cytochrome P-450 and the fdxshe or fdxshe-like protein. The invention further relates to the use of such microorganisms in processes designed for production of pravastatin for use in treatment of disease such as hypercholesterolemia and hyperlipidemia. | 2009-01-01 |
20090004696 | Stabilized bioactive peptides and methods of identification, synthesis, and use - An intracellular selection system allows screening for peptide bioactivity and stability. Randomized recombinant peptides are screened for bioactivity in a tightly regulated expression system, preferably derived from the wild-type lac operon. Bioactive peptides thus identified are inherently protease- and peptidase-resistant. Also provided are bioactive peptides stabilized by a stabilizing group at the N-terminus, the C-terminus, or both. The stabilizing group can be a small stable protein, such as the Rop protein, glutathione sulfotransferase, thioredoxin, maltose binding protein, or glutathione reductase, an α-helical moiety, or one or more proline residues. | 2009-01-01 |
20090004697 | Generation of Chemical Building Blocks from Plant Biomass by Selective Depolymerization - The invention concerns a method for the enzymatic treatment of raw polymeric feedstock comprising the following steps: (a) preferably separation of soluble components from the raw polymeric feedstock, (b) treating the raw polymeric feedstock with an enzyme system in order to liberate defined soluble monomeric or oligomeric building blocks from the insoluble raw polymeric feedstock; and (c) separating the defined monomeric or oligomeric building blocks produced in step b) from the remainder of the raw polymeric feedstock. Preferably, the enzyme system used in step b) contains not more than 50%, preferably not more than 20%, more preferably not more than 10%, more preferably not more than 5%, more preferably not more than 2%, more preferably not more than 1% of other enzyme activities apart from the enzyme activity resulting in liberation of said defined monomeric or oligomeric building blocks from the raw polymeric feedstock according to step b). Further aspects of the invention concern the use of “less pure” and thus less costly enzyme systems in subsequent enzymatic treatment steps and methods for determining the optimum sequence of enzymatic treatment steps by analysis of the raw polymeric feedstock used. | 2009-01-01 |
20090004698 | METHODS TO ENHANCE THE ACTIVITY OF LIGNOCELLULOSE-DEGRADING ENZYMES - Methods for hydrolyzing lignocellulose are provided, comprising contacting the lignocellulose with at least one chemical treatment. Methods for pretreating a lignocellulosic material comprising contacting the material with at least one chemical are also provided. Methods for liberating a substance such as an enzyme, a pharmaceutical, or a nutraceutical from plant material are also provided. These methods are more efficient, more economical, and less toxic than current methods. | 2009-01-01 |
20090004699 | Process for Producing Beta-Chitin - The method of the present invention for producing β-chitin comprises a step of cultivating an alga capable of producing β-chitin using a medium that contains nitrogen (N), phosphorus (P), silicon (Si), and selenium (Se), wherein the weight ratio of phosphorus to nitrogen (P/N) is 0.1 or above, the weight ratio of silicon to nitrogen (Si/N) is 0.1 or above, and nitrogen (N) is contained at 160 ppm or more. It is preferable that selenium (Se) is present in the medium at a concentration of 0.08 ppm to 0.008 ppb. | 2009-01-01 |
20090004700 | Chemical Induction in Quiescence in Bacteria - Quiescence is induced in cells using indole compounds. Expression continues from extra-chromosomal vectors within the cells during quiescence, while chromosomal expression is suppressed. The cells may be used as factories for the production of large amounts of polypeptides of interest, particularly polypeptides which normally have an adverse effect on cell viability or growth. Expression from an extra-chromosomal vector of interest may be monitored, in view of the reduced background expression from the chromosome. Vector copy number may be amplified. Cell cycles may be synchronized. | 2009-01-01 |
20090004701 | Multiplex oligonucleotide addition and target amplification - Methods for appending oligonucleotides directly to nucleic acid templates, particularly to defined sites internal to single-stranded templates, are described. Appending first and second common priming sites to each of a plurality of templates of distinct sequence allows the subsequent stoichiometric amplification of a plurality of templates of distinct sequence. | 2009-01-01 |
20090004702 | CODON SPECIFIC MUTAGENESIS - Materials and Methods are provided for replacing one or more amino acids in a polypeptide with an amino acid of choice to form mutant proteins. Both naturally and non-naturally occurring amino acids can be inserted. A population of mutant proteins can be created in which an amino acid residue has replaced an existing residue at random locations along the primary sequence of the protein. The provided techniques allow for the study of proteins and development of proteins with improved functionalities. | 2009-01-01 |
20090004703 | Method for the Production of Suitable Dna Constructs for Specific Inhibition of Gene Expression by Rna Interference - The invention relates to a method for the production of vectors which, following transfection thereof in eukaryotic cells, are suitable for targeted inhibition of the formation of defined proteins therein by RNA interference. The method for the production of such vectors does not include any PCR steps. It is a three-step procedure in a single reaction vessel and can be carried out within a few hours. Thus, a method is provided which allows very easy testing of a wide variety of siRNA sequences for their functionality within a very short time. Screening processes utilizing the rapid and uncomplicated production of vectors with the aid of said kit can be performed in a cost- and time-saving manner. Another advantage of vectors thus produced is their small size which, among other things, facilitates transfection. | 2009-01-01 |
20090004704 | XYLITOL DEHYDROGENASE-INACTIVATED AND ARABINOSE REDUCTASE-INHIBITED MUTANT OF CANDIDA TROPICALIS, METHOD OF PRODUCING HIGH-YIELD OF XYLITOL USING THE SAME, AND XYLITOL PRODUCED THEREBY - Disclosed herein are Xylitol dehydrogenase-inactivated and arabinose reductase-inhibited mutant of | 2009-01-01 |
20090004705 | PSOD Expression Units - The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms. | 2009-01-01 |
20090004706 | METHODS TO ENHANCE THE ACTIVITY OF LIGNOCELLULOSE-DEGRADING ENZYMES - Methods for hydrolyzing lignocellulose are provided, comprising contacting the lignocellulose with at least one chemical treatment. Methods for pretreating a lignocellulosic material comprising contacting the material with at least one chemical are also provided. Methods for liberating a substance such as an enzyme, a pharmaceutical, or a nutraceutical from plant material are also provided. These methods are more efficient, more economical, and less toxic than current methods. | 2009-01-01 |
20090004707 | Process for Producing Glycolic Acid from Formaldehyde And Hydrogen Cyanide - A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from | 2009-01-01 |