Class / Patent application number | Description | Number of patent applications / Date published |
536550300 | Processes | 17 |
20080207892 | NUCLEIC ACID AMPLIFICATION METHOD USING MICROCHIP AND MICROCHIP, AND NUCLEIC ACID AMPLIFICATION SYSTEM USING THE SAME - A nucleic acid amplification method, includes: performing nucleic acid amplification using a microchip that comprises: a specimen introduction section; a reaction section; and a channel that connects the reaction section and the specimen introduction section, wherein the method further comprises preventing a reaction solution from evaporating during the amplification reaction, and a microchip, includes: a specimen introduction section; a reaction section; and a channel that connects the reaction section and the specimen introduction section, wherein the microchip executes a method for preventing a specimen containing a nucleic acid from evaporating. | 08-28-2008 |
20090043087 | DNA purification and recovery from high particulate and solids samples - This invention relates to methods for rapid nucleic acid purification from sources heavily contaminated with high particulate material, such as cellular debris, and solids, including suspended solids. In particular, this invention provides methods for rapid, quantifiable recovery and purification of nucleic acids from a variety of sources heavily contaminated with solids, such as small organisms, tissue samples, samples of blood found on soil, or samples of washing from foods, which are frequently difficult sources for nucleic acid isolation due to their propensity to clog filters and columns. A device and kit are also provided. | 02-12-2009 |
20090048440 | Nucleotide Sugar Purification Using Membranes - The invention provides methods of removing contaminants from a mixture of a desired product and contaminants by pH adjustments and molecular weight cut-offs. The contaminants include phosphate groups, magnesium sulfate, sodium pyruvate and tetrasodium pyrophosphate groups. The desired product includes nucleotide sugars, glycolipids, LnNT, sialyl lactose, and salts. | 02-19-2009 |
20090137792 | Production Of L-Iduronate Containing Polysaccharides - The present invention provides processes for the production of polysaccharides containing the L-iduronate subunit, for example, heparin-type polysaccharides. New intermediate compounds and processes developed during the production of said polysaccharides are also presented. | 05-28-2009 |
20090216006 | COVALENTLY BOUND POLYSACCHARIDE-BASED CHIRAL STATIONARY PHASES AND METHOD FOR THEIR PREPARATION - The present invention relates to liquid chromatographic chiral stationary phases (CSPs) and their preparation. The CSPs are based on carbamate-derivatized polysaccharides that are covalently bound onto inorganic oxide carriers via unique linkage chemistry. The present invention also relates to methods of obtaining the said linkages, which include derivatizing and functionalizing the polysaccharides, and also chemically bonding the functionalized carbamate-derivatized polysaccharides onto inorganic oxide carriers. The polysaccharide derivatives so obtained can be used as materials for the liquid chromatographic chiral separation of enantiomers. The preferred inorganic oxides are silica, zirconium oxide, and aluminum oxide. Cellulose and amylose are the preferred chiral polysaccharides. | 08-27-2009 |
20100063268 | NUCLEIC ACID EXTRACTION METHOD - A method for extracting a nucleic acid, which comprises: (a) preparing a biomaterial containing a solution by a following step (i) or (ii): (i) a step in which a biomaterial containing a phosphate buffer solution or a Bis-Tris (N,N-bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane) buffer solution is prepared; or (ii) a step in which a buffer solution contained in a biomaterial is replaced with a Bis-Tris buffer solution; (b) dissolving the biomaterial with a lysis solution, and eluting a nucleic acid contained in the biomaterial; (c) preparing a lysate solution by adding a water-soluble organic solvent to the nucleic acid-eluted solution obtained in the step (b); (d) allowing the nucleic acid contained in the lysate solution to be adsorbed by a solid material; (e) washing impurities remaining in the solid material and the lysis solution; and (f) desorbing the absorbed nucleic acid from the solid material by a recovering solution. | 03-11-2010 |
20100093994 | REPARATION METHOD OF AN AMINO LINKER OLIGONUCLEOTIDES AND LABELED-LINKER OLIGONUCLEOTIDE THEREFROM - A method for preparing amino linker oligonucleotides is provided, wherein an amino protecting group is efficiently removed from the amino linker oligonucleotides protected by the protecting group, and thereby achieving a high yield of the amino linker oligonucleotides. | 04-15-2010 |
20110040084 | METHOD FOR EXTRACTING SIALIC ACID-CONTAINING COMPOUND FROM PLANT - It is an object of the present invention to provide an extraction method for a sialic acid-containing compound from a natural raw material, which has no risk of contamination with pathogens affecting animals, can achieve mass production, and does not require the use of an organic solvent harmful to the environment and human body. Provided is an extraction method for a water-soluble sialic acid-containing compound, the method comprising: crudely extracting a water-soluble component with water, polyol, or water containing either an acid or an alkali or polyol from a plant body or a processed product of the plant body, in particular, seeds of cereal or seeds of bean; and separating and recovering the water-soluble sialic acid-containing compound from the resultant crude extract solution with a column packed with a serotonin affinity support. | 02-17-2011 |
20110046364 | METHOD OF LABELING SUGAR CHAIN - The present invention relates to a method of labeling a sugar chain, used for labeling a sugar chain contained in a biological sample, aimed at simplifying a process of isolating a sugar chain from a biological sample and labeling it, which includes (a) trapping a sugar chain in a sugar-trapping substance, which is a substance for specifically trapping a sugar chain from a biological sample; (b) washing the sugar-trapping substance having the sugar chains trapped thereon; (c) releasing the sugar chain from the sugar-trapping substance; and (d) labeling the released sugar chain, | 02-24-2011 |
20110130560 | SONICATION CARTRIDGE FOR NUCLEIC ACID EXTRACTION - A cartridge in which sonication is applied to biological matter to disrupt and release nucleic acids from the matter is formed from a cartridge body containing a series of wells connected by fluid passages engineered to prevent backflow, with at least one well containing a sonication window covered by a thin lamina to transmit sonic vibrations from a sonication horn contacting the exterior surface of the window. Fluid transport among the wells is achieved by pressure differentials through the fluid passages, and a succession of functions is performed in the various wells, including disruption, mixing, binding of the released nucleic acids to binding materials, washing, elution, and collection. | 06-02-2011 |
20110213137 | ISOTOPICALLY-LABELED GLYCANS - The present disclosure provides methods of making isotopically-labeled glycans or a mixture of glycans (e.g., a glycan preparation) via reductive amination. Such methods are useful, for example, in the determination of the relative amounts of a glycan species present in two or more glycoprotein preparations using mass spectroscopy. In one aspect, the present disclosure provides a method of making an isotopically-labeled glycan by a glycan with an amine and an isotopically-labeled reducing agent to provide an isotopically-labeled aminated glycan. In certain embodiments, the amine is an isotopically-labeled amine. | 09-01-2011 |
20110224424 | Method for preparing oligonucleotides - A solution phase synthesis method for preparing an oligonucleotide, wherein at least some of the reagents are solid supported. The method suitable for large- scale synthesis comprises coupling a protected compound with a nucleotide derivative having a protection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond; optionally processing the elongated oligonucleotide by capping by reaction with a solid supported capping agent and/or by oxidizing or sulfurizing by reaction of the oligonucleotide with a solid supported oxidizing or sulfurization reagent; and removing the protection group. The coupling may include reacting a 3′-protected compound of formula: | 09-15-2011 |
20120095205 | Method of Modification of Hyaluronic Acid by Means of (O-ACYL-O'-ALKYL Carbonate-Substituted Pyridine) Complex - This invention relates to a novel method of preparation of hyaluronic acid derivatives by means of a reaction of the hyaluronic acid with the complex (O-acyl-O′-alkyl carbonate-substituted pyridine) of the general formula R—CO—O—CO—O—R1 and R25C5N. The reaction takes place in DMSO in the presence of an external base, forming O-acylated products. The method leads to higher substitution degrees and shorter reaction times compared to the known analogues, hi case the agent comprises two or more functional groups R(CO—O—CO—O—R1)n, crosslinked hyaluronic acid derivatives are formed. | 04-19-2012 |
20120157672 | POROUS STRUCTURE - A porous structure includes a polysaccharide and having a multitude of pores wherein an average pore size of the pores is not smaller than 40 μm and a ratio of the number of pores having a pore size not smaller than 50 μm to the total number of the pores is not less than 30%. The porous structure improves the solubility of a polysaccharide in water. | 06-21-2012 |
20150073134 | Metal Oxide Particles with Uniform Multilayer Polymer Coatings - The present invention is a multilayered composite comprising porous metal oxide particles that are covalently bonded by way of inorganic ether groups to one or more sites of a first polyhydroxyl-functionalized polymer. This first polymer is in turn covalently bonded by way of inorganic ether groups to one or more sites of a second polyhydroxyl-functionalized polymer. The multilayered composites can be prepared by contacting porous inorganic-oxide particles with a sufficient amount of OH-reactive crosslinking agent to form metal oxide particles imbibed with the crosslinking agent, and then contacting the inorganic-oxide particles with a solution of polyhydroxyl-functionalized polymer under reactive conditions. | 03-12-2015 |
20150329579 | METHOD FOR MAKING N-SUBSTITUTED MANNOSAMINE DERIVATIVES - A method for making an N-substituted D-mannosamine derivative of the following formula 1 and its salts | 11-19-2015 |
20150376600 | RNA PREPARATION METHOD - An object of the present invention is to provide a technique for preparing RNA ready for an enzymatic reaction more easily than conventional techniques. The present invention provides a reagent for RNA extraction from a biological sample which contains an alkali metal salt and a surfactant. | 12-31-2015 |