Entries |
Document | Title | Date |
20080275228 | Method and Apparatus of Automatically Isolating and Purifying Nucleic Acid - A method of automatically isolating and purifying nucleic acid from a nucleic acid-containing specimen is provided, the method comprising: injecting a liquid into a cartridge for isolation and purification of a nucleic acid including at least two openings from one opening of the at least two openings, in which the cartridge includes a container having the at least two openings and containing a nucleic acid-adsorbent solid phase; passing the liquid through the nucleic acid-adsorbent solid phase by a pressure difference generated by a pressure generation means for generating a pressure difference between the inside and outside of the container; and discharging the liquid from the other opening of the container to the outside of the container by a pressure difference generated by the pressure generation means, wherein a pressure generated in the inside of the container by the pressure generation means is measured, a pressure change velocity and a pressure change acceleration are calculated on the basis of the value of the measured pressure, and the timing of completion of discharge of the liquid from the container is determined by use of a temporal change pattern of at least one of the measured pressure, the pressure change velocity and the pressure change acceleration. | 11-06-2008 |
20080281089 | Methods and Systems for Differential Extraction - Methods are provided for differential extraction of DNA from at least two different cell types. Systems for carrying out the differential extraction methods are also provided. A kit is also provided for differential extraction of DNA from at least two different cell types using a multi-compartment container. | 11-13-2008 |
20080287669 | METHODS AND COMPOSITIONS FOR IDENTIFYING COMPOUNDS USEFUL IN NUCLEIC ACID PURIFICATION - The present invention provides methods, compositions, and kits for identifying compounds useful in nucleic acid purification. The methods of the invention include identifying certain characteristics of organic solvents such as miscibility in water, dielectric constant, and the class of the solvent. | 11-20-2008 |
20080293931 | Methods and compositions for the removal of nucleic acid amplification inhibitors - An improved method for preparing nucleic acid extracts from environmental samples contaminated by polymerase inhibitors such as humic and fulvic acids is provided. The methods of the invention utilize chemical compositions capable of precipitating humic and/or fulvic acids from organic samples. The methods may be used in connection with the preparation of DNA and RNA extracts, thereby providing purified preparations suitable for amplification using PCR, RT-PCR, and other nucleic acid amplification technologies. Reagent kits for preparing a purified nucleic acid-containing extract from an environmental sample of soil, fluid, or organic particles, using the chemical compositions of the invention are also provided. | 11-27-2008 |
20080300396 | lSOLATION OF NUCLEIC ACID - The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be couple with a further step to isolate RNA from the same sample. | 12-04-2008 |
20090018323 | METHOD FOR EXTRACTING NUCLEIC ACID FROM BLOOD - The present invention relates to an improved method for isolating nucleic acids, particularly genomic desoxyribonucleic acid (DNA) from blood. | 01-15-2009 |
20090036665 | Disposable Sample Processing Unit - A low-cost, non-instrumented, easy-to-use disposable platform for extraction, stabilization, and preservation of viral RNA in specimens at the point of collection is described. The system may use chemical heating. The platform performs the following steps: specimen lysis, RNA extraction, and RNA stabilization in a modular approach. This modular approach confers versatility to the product for application to multiple targets such as avian flu, and HIV, specimens such as blood, nasal swabs, and downstream applications such as PCR or transcription-mediated amplification. The technology described is a point-of-care specimen-processing platform generically applicable to both emerging point-of-care and central-facility molecular diagnostic tests, as well as to surveillance applications. | 02-05-2009 |
20090048438 | Method for washing a column and method for extracting membrane-bound target molecules - A method for washing a column is characterised by using a washing liquid, such as an eluting buffer, water, or even any expected buffer, between the traditional washing step and the eluting step as to effectively remove the impurities and/or residual washing liquid in a column from the previous washing step. A method for extracting membrane-bound target molecules containing the additional washing method is also devised. | 02-19-2009 |
20090048439 | Isolation of nucleic acids molecules using modified solid supports - Provided are solid supports that contain at least one hydrophilic ligand; and at least one hydrophobic ligand, where amount of the at least one hydrophobic ligand on the solid support relative to the amount of the at least one hydrophilic ligand on the solid support is adjusted for binding target nucleic acid(s) from a sample onto the solid support and/or for eluting the bound target nucleic acid(s) from the solid support, so that the amount of target nucleic acid(s) bound to the solid support and/or recovered after elution from the solid support is about 5% to about 500% greater than the amount of target nucleic acid(s) bound to the solid support and/or recovered from the solid support in the absence of either the at least one hydrophobic ligand or the at least one hydrophilic ligand or both. The solid supports with ligands are used for isolation of nucleic acid molecules from samples. | 02-19-2009 |
20090062522 | MONOLITHIC-SHAPED BODIES FOR PURIFYING AND SEPARATING BIOPOLYMERS - The present invention concerns new monolithic shaped bodies, a method for their preparation as well as their use especially for the selective purification and separation of biopolymers. A chromatographic separation material is provided with the new monolithic shaped bodies that allows a selective, efficient and reproducible purification and separation of biopolymers. | 03-05-2009 |
20090069554 | METHOD FOR ISOLATING NUCLEIC ACIDS COMPRISING THE USE OF ETHYLENE GLYCOL MULTIMERS - The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a solid support, preferably magnetic beads, in the presence of an ethylene glycol multimer consisting of from 2 to 70 ethylene oxide monomers, preferably tetraethylene glycol, whereby soluble nucleic acid in said sample is bound to the surface of the support, and separating said support with bound nucleic acid from the sample. Kits for performance of the invention are also provided. | 03-12-2009 |
20090069555 | DEVICE AND PROCEDURE FOR AUTOMATED ISOLATION AND PURIFICATION OF NUCLEIC ACIDS FROM COMPLEX STARTING MATERIALS OF THE USERS CHOICE - A reaction unit containing a bottom part and a top part for pipetting a liquid, wherein the bottom part contains a reaction cavity with a permeable filter grid insert and the top part contains a reaction cavity which can be fixed on said bottom part and contains a cavity or covering for receiving a magnet. | 03-12-2009 |
20090111978 | NON-DESTRUCTIVE PROCEDURE FOR THE ISOLATION OF DNA FROM PLANTS - Provided is a method for obtaining DNA from a plant by collecting the root border cells from a growing root and extracting DNA from the root border cells. Preferably, the root border cells are contained in the root exudate of the growing root, which is growing in a medium, for example, water, tissue culture medium, or soil. Suitably, the root is part of a germinating seed, or the root of a seedling, or the adventitious root of a tissue culture plant or plant part. | 04-30-2009 |
20090149646 | Isolation of Nucleic Acid - The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be coupled with a further step to isolate RNA from the same sample. | 06-11-2009 |
20090209752 | DEVICE AND METHOD FOR TREATING OR CLEANING SAMPLE MATERIAL, IN PARTICULAR NUCLEIC ACIDS - The invention relates to a device and a method for cleaning sample material, in particular nucleic acids. The cleaning process takes place by means of centrifugal microfluidics and the invention provides a simple compact construction and a simple procedure. If necessary, the sample material is easily mixed with a solvent buffer and proteases in the device. The cleaned sample material is in particular transferred directly to a container. Separate containers for the removal of excess liquid are not required. | 08-20-2009 |
20090253903 | METHOD FOR PARALLEL ISOLATION OF VIRAL NUCLEIC ACIDS - Parallel isolation of a double-stranded nucleic acid and a single-stranded nucleic acid is possible from a sample that contains these acids, without separating the acids, by mixing the sample with a lysis buffer having high salt concentration or low salt concentration, or having a proteolytic enzyme. The sample that contains nucleic acid before its lysis, or the sample that has already been lysed or homogenized, is adjusted with a binding buffer in such a manner that the total nucleic acid is adsorbed onto a solid carrier. The binding buffer contains at least one non-ionic detergent in a high concentration. With the exception of the detergent, the sample contains no other non-acidic organic component miscible in water. The carrier with the adsorbed total nucleic acid is removed. The adsorbed total nucleic acid is washed and eluted. | 10-08-2009 |
20090270603 | Method for separating and purifying nucleic acid - A method for separating and purifying nucleic acid, the method comprising: (1) a step of contacting a sample solution containing nucleic acid with a solid phase to adsorb the nucleic acid on the solid phase; (2) a step of contacting a washing solution with the solid phase to wash the solid phase in a state that the nucleic acid is adsorbed on the solid phase; and (3) a step of contacting a recovering solution with the solid phase to desorb the nucleic acid from the solid phase, wherein the sample solution is prepared by including a step of removing a precipitate component, and adding a surfactant and a water-soluble organic solvent to a supernatant solution of the precipitate. | 10-29-2009 |
20090306359 | NON-ALCOHOLIC BUFFER FORMULATIONS FOR ISOLATING, PURIFYING AND RECOVERING LONG-CHAIN AND SHORT-CHAIN NUCLEIC ACIDS - Embodiments relate to methods and formulations of buffers used for isolating, purifying, and recovering long-chain and short-chain nucleic acids. The areas of application of the inventive method include all laboratories engaged in isolating nucleic acids. In one embodiment a solution containing a nucleic acid is prepared with additives containing monovalent and multivalent cations and, optionally, an alcohol and/or additional additives. The solution is contacted with a solid phase, the solid phase is optionally washed, and the nucleic acid is removed. The solution may contain multivalent and/or monovalent cations and may contain an alcohol. The solution in certain embodiments has a pH between 7 and 10. Ammonium chloride, sodium chloride and/or potassium chloride may be used as monovalent salt components. Magnesium chloride, calcium chloride, zinc chloride and/or manganese chloride may be used as multivalent salt components. In a preferred embodiment, identical molar amounts of sodium chloride and manganese chloride are used. | 12-10-2009 |
20100029925 | METHODS AND KITS FOR EXTRACTION OF DNA - Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping. | 02-04-2010 |
20100036109 | METHOD FOR SELECTIVELY AND REVERSIBLY ADSORBING NUCLEIC ACIDS ON A CARRIER MATERIAL - The invention relates to a process for selective, reversible adsorption of nucleic acids to a support material, which process is characterized in that (a) a nucleic acid-containing mixture having a cosmotropic salt content of at least 1 mole is contacted with said support material, whereupon the nucleic acid(s) is (are) adsorbed from said nucleic acid-containing mixture to said support material, and (b) the nucleic acid(s) bound to the support material is (are) detached from the support material by means of an aqueous solution having a salt content of no more than 1 mole. | 02-11-2010 |
20100121046 | COLLECTING AND PROCESSING COMPLEX MACROMOLECULAR MIXTURES - This document provides methods and materials involved in collecting and processing complex macromolecular mixtures (e.g., stool samples). For example, stool collection devices, buffers for stabilizing nucleic acid and polypeptides present in stool, and kits for using sequence-specific capture probes (e.g., nucleic acid sequences designed to hybridize with particular target nucleic acids) to capture target nucleic acids directly from complex macromolecular mixtures (e.g., stool samples) without the need to perform prior steps to enrich, isolate, or purify the nucleic acid component are provided. | 05-13-2010 |
20100130732 | SYSTEM AND METHOD FOR PROCESSING TARGET MATERIAL - A system for processing a target material includes; a cartridge which stores a material which reacts with the target material, and may include at least one chamber and at least one valve connected to the at least one chamber, a first module which may be loaded with the at least one cartridge and may rotate, a second module which may selectively open or close the at least one valve, a third module which may selectively control the temperature of the at least one chamber, and a control module which may control the first module, the second module and the third module. | 05-27-2010 |
20100137574 | SYSTEM AND METHOD FOR THE AUTOMATED EXTRACTION OF NUCLEIC ACIDS - A system and method for the automated extraction of nucleic acids from nucleic acids containing samples are disclosed. In the system, reagents provided in cavities of a reagent plate are transferred to samples accommodated in a process plate using a pipetting device comprising pipettes provided with disposable pipette tips for pipetting of the reagents to obtain sample-reagent mixtures, the sample-reagent mixtures accommodated in the process plate are incubated by an incubating device to release the nucleic acids; the released nucleic acids accommodated in the process plate are separated by a separating device and released to obtain extracted nucleic acids containing fluids; the extracted nucleic acids containing fluids are transferred to cavities of an output plate by disposable pipette tips; wherein each of the samples is assigned one-to-one to a cavity of the reagent plate, at least one of the disposable pipette tips and a cavity of the output plate. | 06-03-2010 |
20100137575 | UNIVERSAL BIOLOGICAL SAMPLE PROCESSING - A method for preparing a sample by utilizing a mechanical force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range. | 06-03-2010 |
20100179310 | GENETIC ANALYSIS OF CELLS - Some aspects relate to methods for genetic analysis of selected cells from within a heterogeneous population of cells. The population of cells first can be partitioned. Selected cells are identified by imaging, and then specifically targeted and lysed by irradiation with an energy beam, resulting in specific release of their cellular contents into the culture medium. The culture medium then can be sampled and assayed for the desired nucleic acids. | 07-15-2010 |
20100197903 | METHOD FOR ISOLATING A NUCLEIC ACID USING PARTICULATE MATTER AND A COMPOSITION THEREFOR - Disclosed are a method for isolating a nucleic acid using particulate matter and a composition therefor. The method comprises essentially the steps of adding a lysis buffer and a neutralization buffer to a biological sample sequentially, and centrifuging cell lysates for at least 10 minutes to separate a solution comprising the nucleic acid and insoluble aggregate comprising denatured protein aggregate and cell debris. The particulate matter is added to and participates in co-aggregation and co-precipitation of denatured protein aggregate and cell debris, and co-aggregates and co-precipitates denatured protein aggregate and cell debris within a much shortened time, thereby to shorten the time and to increase the yield for the isolation of a nucleic acid, compared with conventional methods. | 08-05-2010 |
20100204462 | PIPETTE TIP WITH SEPARATION MATERIAL - The present invention provides an attachment or extension for pipettes and pipette tips. The attachment comprises separation materials which are preferably suitable for isolating nucleic acids from liquids. The separation materials are present in the form of a filter disc in a holder which only slightly increases the pipette volume and thus generates little dead volume. | 08-12-2010 |
20100222564 | METHODS AND COMPOSITIONS FOR ISOLATING SMALL RNA MOLECULES - The present invention concerns the use of methods and compositions for the isolation of small RNA molecules (100 nucleotides or fewer), such as microRNA and siRNA molecules. Such molecules are routinely lost in commonly used isolation procedures and therefore the present invention allows for a much higher level of enrichment or isolation of these small RNA molecules. | 09-02-2010 |
20100280233 | METHOD FOR SAMPLE PREPARATION - A method for preparing a sample by utilizing a mechanical force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range. | 11-04-2010 |
20100305312 | Method For The Extraction And Purification Of Nucleic Acids On A Membrane - The invention relates to a method for the extraction and detection of nucleic acids on a membrane, making it possible to identify the microorganisms present in low concentration in a liquid or gaseous medium, as well as a novel lysis composition comprising guanidium chloride and between 0.1 and 1% N-Lauroyl-Sarcosine (NLS) making it possible to implement this method. | 12-02-2010 |
20110009608 | AUTOMATIC REFINING APPARATUS, MULTI-WELL PLATE KIT AND METHOD FOR EXTRACTING HEXANE FROM BIOLOGICAL SAMPLES - The present invention relates to an automatic refining apparatus for separating target materials from a plurality of biological sample solutions by using magnetic particles to which the magnetic particles are to be reversibly coupled, and to a multi-well plate kit for use in the automatic refining apparatus. Further, the present invention relates to a method for extracting nucleic acids from biological samples by using the above-described automatic refining apparatus. The present invention can be used in the automatic separation of nucleic acid, protein, and the like from biological samples. | 01-13-2011 |
20110015381 | INSTRUMENT AND METHOD FOR COLLECTING NUCLEIC ACIDS - An instrument and a method for conveniently collecting nucleic acids from a biological nucleic acid-containing sample are provided. A nucleic acid-capturing tip having silica-containing solid phases enclosed therein in such a state as being capable of coming into contact with a liquid, wherein the solid phases have a water-flowing regions and the average interval among solid phases in the water-flowing regions is regulated to 25 μm or less. | 01-20-2011 |
20110046361 | METHOD FOR SEPARATION OF DOUBLE-STRANDED AND SINGLE-STRANDED NUCLEIC ACIDS - The invention provides systems, methods and kits for the separation and/or purification of double-stranded and single-stranded nucleic acids. The method includes first mixing a sample containing the double-stranded nucleic acid and the single-stranded nucleic acid with a pH-neutral, buffered solution consisting essentially of a chaotropic salt and a pH buffer to generate a mixture; then applying the mixture to a first mineral support for the double-stranded nucleic acid to bind; and collecting the flow-through which contains unbound single-stranded nucleic acid. The method further includes adjusting the pH of the flow-through to an acidic pH, and applying the acidified flow-through to a second mineral support for the single-stranded nucleic acid to bind. Alternatively the flow-through can be mixed with a lower aliphatic alcohol prior to loading of the second column. The double-stranded and the single-stranded nucleic acids bound can be eluted from the mineral supports respectively. | 02-24-2011 |
20110087016 | System and Method for Emulsion Breaking and Recovery of Biological Elements - An embodiment of a method for extracting biological material from an emulsion is described that comprises the steps of a) breaking an emulsion comprising a plurality of aqueous droplets in a continuous phase of an oil using a solvent to produce a combined aqueous-oil mixture, where the solvent disrupts the aqueous droplets which release a plurality of biological elements each immobilized on a substrate into the combined aqueous-oil mixture; b) introducing an inorganic salt to the combined aqueous-oil mixture causing a phase separation of the mixture into a first phase comprising an aqueous solution and the biological elements and a second phase comprising the solvent and the oil; c) extracting the first phase from the second phase; and d) collecting the substrate immobilized biological elements from the first phase. | 04-14-2011 |
20110184162 | METHOD FOR RAPID ISOLATION OF RNA AND A KIT THEREOF - The present invention relates to a method for rapid isolation of RNA. More particularly, it relates to a method for isolation of RNA using two-solution system. The present invention also relates to a RNA isolation kit. | 07-28-2011 |
20110196146 | METHODS, COMPOSITIONS, AND KITS FOR RECOVERY OF NUCLEIC ACIDS OR PROTEINS FROM FIXED TISSUE SAMPLES - Methods and materials for improving nucleic acid or protein recovery from samples preserved in liquid cytological preservative solutions by utilizing scavenging agents, such as hydrazine- and hydrazide-containing compounds, are provided. Lysis solutions comprising hydrazine- and hydrazide-containing compounds and kits comprising the same are also provided. | 08-11-2011 |
20110224419 | Method For Isolating And Purifying Nucleic Acids - The present invention relates to a method for the isolation and purification of nucleic acids by elution of nucleic acids from nucleic acid-containing samples, and biological materials. The present invention further relates to a kit for carrying out the method of the invention. | 09-15-2011 |
20110224420 | SOLID SUPPORT - The present invention provides a solid support for performing steps of isolation of cell or extraction and purification of nucleic acid, safely, easily, efficiently, and with high yield in the genetic test for investigating the presence of pathogenic bacterial infection. A solid support for binding with cell as an embodiment of the above-described solid support, comprises a polypeptide having capability of binding with my colic acid-containing glycolipid which is immobilized on the surface of a carrier. In addition, a solid support for binding with nucleic acid as another embodiment of the above-described solid support, comprises a polypeptide having capability of binding with nucleic acid which is immobilized on the surface of a carrier. | 09-15-2011 |
20110245483 | Method For Purification Of Nucleic Acids, Particularly From Fixed Tissue - The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution. | 10-06-2011 |
20110313145 | ISOLATION OF RNA - Disclosed herein are methods for purification of RNA from a sample. The RNA can be total RNA or mRNA. The method involves preparing the sample in a solution of lysis buffer and depositing into a first end of a lysis straw such that the sample solution flows through the matrix of the lysis straw and is eluted from the opposite end of the lysis straw, and depositing the eluted material into a first end of a solid phase extraction (SPE) straw, such that the deposited solution flows through the matrix of the SPE straw towards the opposite end of the SPE straw, and eluting the RNA from the SPE straw by depositing a solution of elution buffer, into the first end of the SPE straw, such that the deposited solution flows through the matrix of the SPE straw and is eluted from the opposite end of the SPE straw, wherein purified RNA from the sample is present in the eluate of the SPE straw. When the RNA is total RNA, and the sample is a cell sample, and step b) requires adding a precipitating solution to the eluted material from step a), and depositing the solution into the first end of the SPE straw, wherein the straw comprises silica microspheres, such that the deposited solution flows through the matrix of the SPE straw toward the opposite end of the SPE straw. When the RNA is mRNA and step b) further comprises depositing the solution into the first end of the solid phase extraction (SPE) straw, wherein the straw comprises oligo-dT. Pressure (e.g., at least 10 psi pressure) and heat (e.g., about 60° C.) can be applied to the samples, and/or eluates and/or straws. Examples of lysis buffers, loading buffers and elution buffers are provided. Also disclosed are the specific straws and porous polymer monolith matrix components. | 12-22-2011 |
20120022247 | METHODS OF PURIFYING A NUCLEIC ACID AND FORMULATION AND KIT FOR USE IN PERFORMING SUCH METHODS - A formulation containing guanidine thiocyanate together with acetamide, one or more acetamide derivatives, or a combination of acetamide and one or more acetamide derivatives is used to purify one or more nucleic acids contained in a medium. In particular, a medium containing at least one nucleic acid is combined with a binding matrix and the formulation in order to cause the at least one nucleic acid to separate from its in vivo cellular environment and to bind to the binding matrix. The binding matrix with at least one nucleic acid bound thereto then is separated from substantially the rest of the combined medium and formulation, after which the at least one nucleic acid is eluted from the binding matrix to obtain the at least one nucleic acid in a substantially purified form. | 01-26-2012 |
20120077969 | NUCLEIC ACID PURIFICATION METHOD - The present invention relates to a method for purifying a defined amount of nucleic acids from a nucleic acid-containing sample, which has at least the following steps: (a.) contacting the nucleic acid-containing sample with a defined amount of a nucleic acid binding phase with the following features: (i) the nucleic acid binding phase has nucleic acid binding ligands that have at least one protonatable group; (ii) the nucleic acid binding ligands are bound to a carrier; (iii) the nucleic acid binding phase has a surface with a low charge density, wherein the amount of nucleic acids in the sample exceeds the binding capacity of the amount of nucleic acid binding phase used; (b.) binding of the nucleic acids to the nucleic acid binding phase at a pH (binding pH) that is below the pKs value of at least one of the protonatable groups; (c.) elution of the nucleic acids at a pH that is above the binding pH, wherein a defined amount of nucleic acids is obtained. Furthermore, corresponding kits and nucleic acid binding phases, which can be used for the purification of nucleic acids, are disclosed. | 03-29-2012 |
20120123110 | Plasmid DNA Extraction Process - A process for the extraction of pDNA from cells is provided. In one aspect, the process comprises heating a liquid comprising the cells to an average temperature of from 95° C. to about 120° C. for a time of less than 10 seconds. In certain preferred aspects, the pDNA is extracted by the use of flow-through apparatus. | 05-17-2012 |
20120130061 | Method FMethod For Isolating And Purifying Nucleic Acids - The present invention relates to a method for the isolation and purification of nucleic acids by elution of nucleic acids from nucleic acid-containing samples and biological materials. The present invention further relates to a kit for carrying out the method of the invention. | 05-24-2012 |
20120178918 | NUCLEIC ACID PREPARATION COMPOSITIONS AND METHODS - Provided herein are methods and compositions to extract and enrich by, physical separation or amplification, relatively short nucleic acids from a nucleic acid composition containing a high background of longer nucleic acids (e.g., host or maternal nucleic acids; genomic nucleic acid and the like). | 07-12-2012 |
20120197008 | NUCLEIC ACID AND EXTRACTION METHOD - There is provided a nucleic acid extraction method applicable to microbes in a relatively wide range, and capable of rapidly extracting nucleic acid. The nucleic acid extraction method comprises the steps of introducing a cell suspension into a vessel, sealing the vessel, and preheating a heater up to a set temperature not lower than 100° C. Further, the method comprises the step of bringing the vessel into contact with the heater heated up to the set temperature, thereby heating the cell suspension housed in the vessel up to a prescribed highest temperature at not lower than 100° C. with the vessel held in a sealed state. | 08-02-2012 |
20120197009 | ISOLATION OF NUCLEIC ACIDS - A method for extracting nucleic acids from a biological material such as blood comprises contacting the mixture with a material at a pH such that the material is positively charged and will bind negatively charged nucleic acids and then eluting the nucleic acids at a pH when the said materials possess a neutral or negative charge to release the nucleic acids. The nucleic acids can be removed under mildly alkaline conditions to the maintain integrity of the nucleic acids and to allow retrieval of the nucleic acids in reagents that are immediately compatible with either storage or analytical testing. | 08-02-2012 |
20120238739 | METHODS FOR IMPROVED DNA RELEASE FROM BINDING SUBSTRATES AND/OR DECREASING PCR INHIBITION IN PATHOGEN DETECTION - Disclosed herein are processes for collecting nucleic acids from particulate samples. One embodiment disclosed herein relates to the use of ultrasonic energy to simultaneously shear large nucleic acid molecules and large particulates to very small sizes prior to or during a chemical binding step to a nucleic acid binding surface. Another embodiment involves crushing the nucleic acid binding surface prior to eluting the bound nucleic acid molecules to enable better wetting of the nucleic acid binding surface and easier diffusion of bound nucleic acid molecules out of the nucleic acid binding surface. | 09-20-2012 |
20120271043 | PROCESS AND DEVICE FOR COLLECTING NUCLEIC ACIDS OF MICROORGANISMS FROM A PARTICULATE SAMPLE - Disclosed herein are processes and devices for collecting nucleic acids of microorganisms from particulate samples. | 10-25-2012 |
20120296075 | GRAVITY FLOW FLUIDIC DEVICE FOR NUCLEIC ACID EXTRACTION - A system, method, and kit for extracting nucleic acid from a sample containing nucleic acid uses an extraction device with an elongate channel. Fluids are provided to the channel via gravity feed to the inlet port. The flow rate and other flow behavior may be controlled with a siphon provided at the outlet port. | 11-22-2012 |
20120309952 | SMALL RNA PURIFICATION - The present invention relates to methods, kits, and compositions for purifying small RNA molecules. In particular, the present invention provides methods for purifying small RNA molecules from a sample containing both small RNA molecules and larger RNA molecules using a compaction agent and a RNA binding matrix, as well as compositions and kits for practicing such methods. In certain embodiments, the compaction agent comprises a plurality of metal-amine-halide molecules. | 12-06-2012 |
20130005955 | Micro Rotary Machine and Methods for Using Same - A micro rotary machine may include a micro actuator and a micro shaft coupled to the micro actuator. The micro shaft comprises a horizontal shaft and is operable to be rotated by the micro actuator. A micro tool is coupled to the micro shaft and is operable to perform work in response to motion of the micro shaft. | 01-03-2013 |
20130023656 | METHOD FOR SELECTIVE ISOLATION AND PURIFICATION OF NUCLEIC ACIDS - The present invention relates to a gentle method for isolating and purifying nucleic acids from a biological cell comprising sample comprising at least DNA, RNA, and proteins, comprising at least the steps of 1. mixing the sample with a lysis buffer, 2. incubating the sample at a temperature within a range between 45° C. and 59° C. to obtain DNA as well as RNA or within a range between 60° C. and 70° C., to obtain DNA essentially free of RNA and 3. separating the nucleic acids from any contaminants. | 01-24-2013 |
20130041145 | METHOD FOR ISOLATING RNA FROM WHOLE BLOOD SAMPLES - The present invention relates to a method for isolating RNA from a whole blood sample, comprising the steps bringing the sample into contact with an aqueous lysis solution containing at least one lysis substance in a concentration of 1.5 mol/1 to 7 mol/1 and at least one detergent, simultaneously or subsequently adding a proteinase, in particular proteinase K, then incubating the solution for at least partial enzymatic digestion and lysis of the sample so that a lysate is obtained, and isolating the RNA from the lysate. | 02-14-2013 |
20130046086 | METHOD FOR SEPARATING CARBON NANOTUBES - This invention relates methods and apparatus to separate charged species such as carbon nanotubes by their electrical conductivity. Carbon nanotubes of varying electrical conductivity may be separated by hybridizing the nanotubes with nucleic acids such as DNA and then passing a dispersion of the hybrids through an electrochemical cell with an electrical potential on the working electrode which absorbs or desorbs nanotubes of a desired electrical conductivity. | 02-21-2013 |
20130066061 | PURIFICATION OF OLIGONUCLEOTIDES - A method for purifying a protected oligonucleotide comprising the steps of:
| 03-14-2013 |
20130066062 | NUCLEIC ACID EXTRACTION METHOD, NUCLEIC ACID EXTRACTION REAGENT KIT, AND NUCLEIC ACID EXTRACTION REAGENT - A method is provided that is suitable for various test samples and that prepares nucleic acid templates directly usable for gene amplification reaction such as PCR method or RT-PCR method conveniently and promptly, preferably under a mild condition. A nucleic acid extraction method is provided comprising a process of contacting a nucleic acid extraction reagent with a test sample, wherein the nucleic acid extraction reagent comprises zwitterionic buffer solution. Preferably, the nucleic acid extraction reagent comprises a surfactant and a proteolytic enzyme. | 03-14-2013 |
20130096292 | POLYNUCLEOTIDE CAPTURE MATERIALS, AND METHODS OF USING SAME - Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as RNA and/or DNA from such samples. The RNA and/or DNA is captured by polyamindoamine (PAMAM (Generation 0)) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding RNA and of DNA, and of release, and thereby permit quantitative determinations. | 04-18-2013 |
20130144049 | SOLUTION FOR EXTRACTION OF RNA - A solution for extracting substantially pure RNA from a biological sample is disclosed. The solution for extracting RNA from a biological sample containing RNA and at least DNA comprises:
| 06-06-2013 |
20130165643 | NUCLEIC ACID EXTRACTION KIT, NUCLEIC ACID EXTRACTION METHOD, AND NUCLEIC ACID EXTRACTION APPARATUS - A nucleic acid extraction kit, which enables the nucleic acid extraction operation to be accomplished safely without causing contamination, and in which the complex preparation of reagents and the disposal treatments that are performed before and after the nucleic acid extraction operation can be performed rapidly and simply, with the extraction performed in an automated manner. The nucleic acid extraction kit includes: a container including reagent wells that each store at least a reagent, a sample well into which a biological sample is introduced, a waste liquid well, and a collection well in which an extracted nucleic acid is collected, and an extraction filter cartridge equipped with an extraction filter for separating and extracting a nucleic acid from the biological sample, wherein the extraction filter cartridge is formed in a manner that enables the extraction filter cartridge to be supported on the waste liquid well and the collection well. | 06-27-2013 |
20130331560 | METHODS AND DEVICES FOR PRODUCING BIOMOLECULES - A scalable process and device for producing a biomolecule, in particular pharmaceutical grade plasmid DNA. The process includes the steps of alkaline lysis and a neutralization. For separating the lysate and the precipitate, the mixture is allowed to gently flow downward through a clarification reactor that is partially filled, in its lower part, with retention material like glass beads, whereby the precipitate is retained on top of and within the retention. In a preferred embodiment of the lysis step, cell suspension and alkaline lysis solution flow through a lysis reactor that is filled with particulate material like glass beads. The process can be run continuously and fully automated. | 12-12-2013 |
20140018529 | NUCLEIC ACID ISOLATION AND PURIFICATION SYSTEM - A device was developed for use in nucleic acid isolation that includes reversibly connected upper and lower chambers, and contains a fibrous nucleic acid binding surface which can expand in the chambers when wetted. The device may be used in a rapid nucleic acid isolation process without clogging while accommodating complex samples. | 01-16-2014 |
20140039176 | METHODS AND KITS FOR EXTRACTION OF DNA - Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping. | 02-06-2014 |
20140046050 | METHOD FOR ISOLATING NUCLEIC ACIDS AND COMPOSITION USED THEREFOR - The disclosure provides a composition and method for isolating nucleic acids, in which the composition includes at least one halocarbon, at least one salt and at least one surfactant. Mixing the composition and a biosample to form a homogenized solution, nucleic acids in the solution can be easily isolated with a simple treatment and good yield. | 02-13-2014 |
20140051847 | METHOD FOR ISOLATING DNA - The present invention provides a method for the isolation of nucleic acid from microbial cells in an environmental sample. The method includes preparing a suspension of the environmental sample, lysing the suspended sample with a buffered solution, adding sodium dodecylsulfate solution to the lysed suspended sample, carrying out solvent extraction and separation to obtain an aqueous phase, reacting the aqueous phase with solvents to generate an insoluble precipitate containing nucleic acid, and isolating the nucleic acid therefrom, thereby releasing high molecular weight nucleic acid pellets from the cells. | 02-20-2014 |
20140094597 | METHOD FOR ISOLATING NUCLEIC ACIDS COMPRISING THE USE OF ETHYLENE GLYCOL MULTIMERS - The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a solid support, preferably magnetic beads, in the presence of an ethylene glycol multimer consisting of from 2 to 70 ethylene oxide monomers, preferably tetraethylene glycol, whereby soluble nucleic acid in said sample is bound to the surface of the support, and separating said support with bound nucleic acid from the sample. Kits for performance of the invention are also provided. | 04-03-2014 |
20140179909 | MICROFLUIDIC DEVICE FOR NUCLEIC ACID EXTRACTION AND FRACTIONATION - Various embodiments of the present disclosure generally relate to molecular biological protocols, equipment and reagents for the extraction and fractionation of DNA molecules, from whole or lysed samples, in a single flow-through device. | 06-26-2014 |
20140194613 | USE OF MICROVESICLES IN DIAGNOSIS AND PROGNOSIS OF MEDICAL DISEASES AND CONDITIONS - The presently disclosed subject matter is directed to methods of aiding diagnosis, prognosis, monitoring and evaluation of a disease or other medical condition in a subject by detecting a biomarker in microvesicles isolated from a biological sample from the subject. | 07-10-2014 |
20140256929 | EXTRACTION BUFFER AND METHOD FOR ISOLATING HIGH-QUALITY RNA FROM CELLS EXPOSED TO METAL CHLORIDE SOLUTIONS AND CLAY MINERAL SUSPENSIONS - An RNA extraction buffer, an RNA extraction method, and an RNA extraction kit are described which enable functional, rapid, efficient, and high-quality RNA isolation from samples containing high concentrations of aqueous metal cations, clays, silica, or silicate minerals | 09-11-2014 |
20140288293 | COMPOSITIONS AND METHODS FOR NUCLEIC ACID EXTRACTION - Compositions and methods for the efficient extraction, enrichment and isolation of nucleic acids from fresh, fixed or fixed and embedded cells, tissues, biological materials and cellular source material. | 09-25-2014 |
20140323711 | POLYNUCLEOTIDE CAPTURE MATERIALS, AND METHODS OF USING SAME - Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as RNA and/or DNA from such samples. The RNA and/or DNA is captured by polyamindoamine (PAMAM (Generation 0)) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding RNA and of DNA, and of release, and thereby permit quantitative determinations. | 10-30-2014 |
20140336372 | DNA EXTRACTION FROM SEEDS USING OSMOTICUM - The invention broadly relates to methods of fragmenting seed by use of mechanical devices such as crushing pins or other crushing devices with a preconditioned hard seed. Methods of preconditioning the seeds to soften the seed for more effective fragmentation which are adapted to enhance extracted DNA yields and/or DNA quality are shown. Extracted DNA yield from alkali-soaked seeds can be significantly increased by adding an osmoticum to a seed-softening alkali soaking solution used for pretreatment of the seeds prior to crushing them. The osmoticum inhibits and reduces liquid uptake by the seeds, but the seeds are weakened enough to be crushed with steel beads, without requiring the use of crushing pins or other crushing devices. | 11-13-2014 |
20140343268 | METHODS FOR PURIFYING NUCLEIC ACIDS AND DEVICES THEREOF - A nucleic acid purification device includes a housing and an adapter operatively attached to the housing. The adapter is configured to receive at least one well plate which includes a plurality of wells for receiving contents including at least a sample and a plurality of paramagnetic particles. The device also includes a motor disposed within the housing and configured to selectively move at least the adapter to thereby disrupt or shake the contents of the plurality of wells when the at least one well plate is received by the adapter. The device further includes at least one electromagnetic feature configured to selectively receive an electrical signal and thereby magnetize at least a portion of the plurality of paramagnetic particles when the plurality of paramagnetic particles are received by the plurality of wells and the at least one well plate is received by the adapter. | 11-20-2014 |
20150112054 | Method for Isolating and Purifying RNA from Biological Materials - Includes the following steps: (1) Mix organs and tissues with formamide and monovalent cation salt solution, homogenize them to obtain the dehydrated biological sample; (2) Mix the dehydrated biological sample with monovalent cation salt solution for incubation; (3) Add the monovalent cation salt solution with precipitation effect to the mixture, mix them and centrifuge, then pour the supernatant into another centrifuge tube; (4) Add isopropanol, mix it and centrifuge, and then discard the upper phase liquid, the lower phase liquid and the visible residual impurities between the upper and lower phases to get white RNA precipitate. This method proposed in the invention enables efficient isolation of protein from RNA in the biological samples. It avoids decomposition of RNA by the residual protein that remains in the products; the reagent used in the invention is low-toxicity compound with little hazard to the environment and human body; since the products obtained are not easily decomposed, the products can be transported at long distance and stored at room temperature; the method is easy to operate and no special skill is required for the operator. The equipment used is simple and cost efficient and enables high RNA yield and high purity. | 04-23-2015 |
20150126724 | SAMPLE EXTRACTION AND PREPARATION DEVICE - A fluid sample extraction device is disclosed comprising a housing defining an internal fluid passage having a distal open end and a proximal open end and a porous medium within the fluid passage, between the distal open end and proximal open end. The porous medium, which may be glass, is configured to allow fluid flow in the fluid passage to flow around the porous medium, toward the proximal open end of the housing, when fluid is drawn from the distal open end toward the proximal open end, and to allow fluid in the fluid passage to flow toward the distal open end of the housing, through the porous medium when the fluid is forced toward the distal open end. The porous medium is also configured to capture nucleic acids in the porous medium from the fluid when fluid is forced through the porous medium. Devices and kits are also disclosed. | 05-07-2015 |
20150299693 | METHOD AND APPARATUS FOR ISOLATING NUCLEIC ACIDS - A method comprises: sorbing a sample solution comprising nucleic acids to a sample receiving portion of a quartz fiber filter by contacting the sample solution with the sample receiving portion; and washing the sample receiving portion while keeping most of nucleic acids around the sample receiving portion by flowing a wash solution through the sample receiving portion under a wicking force directed away from the sample receiving portion. An associated apparatus is also provided. | 10-22-2015 |
20150353920 | METHODS FOR ISOLATING MICROVESICLES - The invention provides novel methods for isolating microvesicles from a biological sample and for extracting nucleic acids from the microvesicles. | 12-10-2015 |
20160046659 | DEVICE FOR PURIFYING NUCLEIC ACIDS - The invention relates to a device ( | 02-18-2016 |
20160102305 | POLYNUCLEOTIDE CAPTURE MATERIALS, AND METHODS OF USING SAME - Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as RNA and/or DNA from such samples. The RNA and/or DNA is captured by polyamindoamine (PAMAM (Generation 0)) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding RNA and of DNA, and of release, and thereby permit quantitative determinations. | 04-14-2016 |
20160251647 | CONTINUOUS FLOW NUCLEIC ACID EXTRACTION APPARATUS AND METHOD OF USE THEREOF | 09-01-2016 |