Entries |
Document | Title | Date |
20080207888 | METHODS OF SYNTHESIZING AND PRESERVING A NUCLEOTIDE-LABELED MICROTUBULE - A method of synthesizing a nucleotide-labeled microtubule includes causing a microtubule which is stabilized after polymerization to react with a chemical crosslinking agent which has succinimide and maleimide and nucleotides which have a thiolated 3′ end or 5′ end to synthesize a microtubule-chemical crosslinking agent-nucleotide complex. | 08-28-2008 |
20080234474 | METHODS FOR THE SEPARATION OF BIOLOGICAL MOLECULES USING SULFOLANE - The present invention provides a method for the isolation of biological molecules by the adsorption of the molecules onto a mineral substrate in the presence of an appropriate mixture of salts and sufolane. Preferably, the biological molecules are nucleic acids. Compositions and kits for performing the process according to the invention are also provided. | 09-25-2008 |
20080234475 | PROCESS FOR THE SYNTHESIS OF 2'-O-SUBSTITUTED PURINES - The present invention provides an improved process for the synthesis of 2′-O-substituted purine nucleosides. The process includes anhydro or thioanhydro ring opening of a selected 8,2′-cyclopurine nucleoside with a weak nucleophile in the presence of a Lewis acid ester, followed by reduction to afford the desired 2′-O-substituted purine nucleoside. | 09-25-2008 |
20080262212 | Ribocloning: Recombinant DNA Construction using Primers with RIBO Bases - The present invention provides methods of linking nucleic acids without the use of restriction enzymes or any joining enzyme such as ligase. More specifically, the present invention provides methods for cloning or rearranging a double-stranded target DNA which is a PCR product into a double-stranded vector DNA which is a PCR product, said DNAs being amplified using primers that contain at least one ribonucleotide, preferably at or near the respective 3′ ends of the primers, such that RNA-specific cleavage, preferably by RNAse A, will allow release of the primers to create long matching 3′-sticky ends. | 10-23-2008 |
20080281087 | Method For Bisulfite Treatment - The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines. In this method, the nucleic acid is bisulfite treated and is bound to a solid phase when an alkaline solution is added for desulfonation and elution of the nucleic acid from the solid phase. The nucleic acid can be amplified in an additional step. The solid phase is preferably a material comprising glass or silica, more preferably a glass fleece, glass membrane or a magnetic glass particle. Further, several uses of the alkaline solution for bisulfite treatment are disclosed and a kit containing a bisulfite reagent, a solid phase and an alkaline solution. | 11-13-2008 |
20080287668 | Nanostructures and methods of making - A nanostructure can include a first single-stranded nucleic acid, a plurality of complementary single-stranded helper nucleic acids hybridized with the first single-stranded nucleic acid, and at least one nanocube linked to a helper nucleic acid by a tether. The nanostructure can include at least two nanocubes each linked to a helper nucleic acid by a tether, and bonded to each other. | 11-20-2008 |
20080293929 | SYNTHESIS OF SULFURIZED OLIGONUCLEOTIDES - Methods for the formation of sulfurized oligonucleotides are provided. The methods allow for the formation of phosphorothioate linkages in the oligonucleotides or derivatives, without the need for complex solvent mixtures and repeated washing or solvent changes. Oligonucleotides having from about 8, and up to about 50, nucleotides can be sulfurized according to the methods of the invention with higher yields than have been previously reported. | 11-27-2008 |
20090023910 | Applications with and Methods for Producing Selected Interstrand Cross-Links in Nucleic Acids - The invention provides a method for stably cross-linking a first nucleic acid sequence selected for being stably cross-linked in the presence of a second nucleic acid sequence selected for not being stably cross-linked in a nucleic acid molecule. The method comprises providing a nucleic acid sequence that is complementary to the first nucleic acid sequence; hybridizing the first nucleic acid sequence to its complementary sequence; and stably cross-linking the first nucleic acid sequence to the complementary sequence. | 01-22-2009 |
20090036664 | Complex oligonucleotide primer mix - The invention generally relates to a complex mixture of oligonucleotide primers and/or probes. Another aspect of the invention includes a method of selective priming of a target nucleic acid. | 02-05-2009 |
20090043086 | Methods for Synthesizing a Collection of Partially Identical Polynucleotides - Methods for synthesizing a collection of partially identical polynucleotides are disclosed. | 02-12-2009 |
20090048436 | Methods of synthesizing chemically cleavable phosphoramidite linkers - The present invention provides a method of synthesizing phosphoramidite linkers that are useful for the production of synthesizing two or more oligonucleotides in tandem. The inventive linker has the following desirable properties: (i) enhanced stability to alkali conditions versus the linkers previously published, (ii) cleaves to produce 5′ and 3′ ends that are fully biologically compatible, (iii) cleaves completely under conditions that are already used in cleavage/deprotection processes so it is fully compatible with conditions that are common in laboratories and does not require additives that necessitate further purification after cleavage, (iv) integrates easily onto commercially available synthesizers because it is compatible with standard coupling chemistry, and (v) is compatible with DNA, RNA, forward, reverse, and LNA, synthesis chemistries. In addition, the inventive linkers may be coupled to a solid support. Thus, the inventive linkers provide a significant advancement in the state of the art. | 02-19-2009 |
20090131648 | Rigid dendrimeric structures - The present invention provides novel dendrimers having a multimeric structure obtainable by reaction of a core molecule having an adamantoid structure with branching synthons and terminating synthons, said dendrimers immobilised on a support and a method of hybridisation which uses said dendrimers immobilised on a support. | 05-21-2009 |
20090171076 | COMPOSITIONS AND METHODS FOR THE USE OF FMOC DERIVATIVES IN DNA/RNA SYNTHESIS - Methods of nucleic acid preparation are described, including preparation of MnRNA, using FMOC derivatives to synthesize oligonucleotides in addition to applying FMOC protocols to various therapeutic and diagnostic methods. In some embodiments a single stranded oligonucleotide is synthesized bound to a polymer support (such as optic fiber glass filters) using said FMOC derivatives. | 07-02-2009 |
20090171077 | DELIVERY OF NUCLEIC ACID-LIKE COMPOUNDS - A process for preparing a microparticulate complex is provided. The process comprises (a) combining a particle-forming component (“PFC”) and a nucleic acid-like component (“NAC”) in a monophasic composition comprising water and a water-miscible, organic solvent to form a mixture wherein the PFC and the NAC are independently molecularly or micellarly soluble in the aqueous/organic solvent system, and (b) reducing the amount of the organic solvent in the mixture. This effects formation of the microparticulate complex of the NAC and the PFC. Also provided is a microparticulate complex that comprises a particle-forming component complexed to a nucleic acid-like component forming an approximately spherical particle, wherein the particle-forming component encloses an interior of the particle containing the nucleic acid-like component and the so-enclosed interior volume has less than about 50% (preferably less than 20%) of the volume containing free water. Also disclosed composition comprising water and particles of the microparticulate complex. According to the invention a nucleic acid-like component is delivered to a cell by (a) contacting the cell with a composition comprising water and the microparticulate complex, and (b) maintaining the contact for a time sufficient to allow the nucleic acid-based moiety to enter the cell. A therapeutic nucleic acid-like component is delivered into a patient in need thereof by administering a composition comprising water and particles of the microparticulate complex. Also disclosed is a charge-changing composition represented by the formula A-X-B, wherein X represents a chemical bond capable of irreversible dissociation in reaction to a factor in a physiological or bioprocess environment; A represents a molecular moiety that upon dissociation of the bond X produces a ionically charged product; and B represents a molecular moiety, which upon the dissociation of bond X, separates from the composition leaving the remaining ionically charged product more positive than that of A-X-B itself. | 07-02-2009 |
20090209750 | Organic compound synthesizer and method for synthesizing organic compounds - An organic compound synthesizer for synthesizing organic compounds contains at least one type of polymerizable repeat unit and includes a substrate for organic compound synthesis. A liquid supply unit is configured to supply a reaction liquid containing compounds necessary for the synthesis of organic compounds and a reaction liquid containing a thermal acid generator for generating protons by heating. A substrate heater is configured to selectively heat a specific portion of said substrate for organic compound synthesis to thereby heat the reaction liquid containing a thermal acid generator. | 08-20-2009 |
20090209751 | METHODS AND KITS FOR EXTRACTION OF DNA - Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping. | 08-20-2009 |
20090221808 | Process for the Preparation of Nucleic Acid Duplexes - The invention is a process for the preparation of at least one nucleic acid duplex by the steps of: (a) synthesizing a first single strand oligonucleotide to produce a crude solution of the first single strand oligonucleotide; (b) purifying the first single strand oligonucleotide from the crude solution by first liquid chromatography to produce a solution of purified first single strand oligonucleotide in a first liquid chromatography eluant; (c) synthesizing a second single strand oligonucleotide to produce a crude solution of the second single strand oligonucleotide; (d) purifying the second single strand oligonucleotide from the crude solution by second liquid chromatography to produce a solution of purified second single strand oligonucleotide in a second liquid chromatography eluant; (e) mixing the solution of purified first single strand oligonucleotide in the first liquid chromatography eluant with the solution of purified second single strand oligonucleotide in the second liquid chromatography eluant so that the first and second single strand oligonucleotides can complex to form a nucleic acid duplex in the mixture of first and second liquid chromatography eluants. | 09-03-2009 |
20090286970 | METHOD FOR INTRODUCING A NUCLEIC-ACID PROTECTING GROUP - The present invention relates to a simple, economical method for introducing substituent (I) at the 2′-hydroxyl group of the ribose of a ribonucleic acid derivative whose 3′-hydroxyl group and 5′-hydroxyl group are protected with a silicon protecting group, wherein WG | 11-19-2009 |
20090299046 | SELF-ASSEMBLING OLIGONUCLEOTIDES AND METHODS - A composition comprising individual single-stranded oligonucleotides capable of self assembling to form a pair of complementary, substantially contiguous single strands of a double-stranded nucleic acid filament, and a method for forming such filaments are disclosed. | 12-03-2009 |
20090326210 | POROUS RESIN BEADS AND METHOD OF PRODUCING NUCLEIC ACID USING THE SAME - The present invention provides porous resin beads containing an aromatic monovinyl compound-divinyl compound-(meth)acrylamide derivative copolymer. Preferably, the copolymer further contains as a structural unit a second aromatic monovinyl compound having a functional group capable of binding to a carboxyl group by a dehydrating condensation reaction. | 12-31-2009 |
20100004436 | METHODS AND COMPOSITIONS FOR THE SPECIFIC INHIBITION OF GENE EXPRESSION BY DOUBLE-STRANDED RNA - The invention provides compositions and methods for selectively reducing the expression of a gene product from a desired target gene, as well as treating diseases caused by expression of the gene. The method involves introducing into the environment of a cell an amount of a double-stranded RNA (dsRNA) such that a sufficient portion of the dsRNA can enter the cytoplasm of the cell to cause a reduction in the expression of the target gene. The dsRNA has a first oligonucleotide sequence that is between 26 and about 30 nucleotides in length and a second oligonucleotide sequence that anneals to the first sequence under biological conditions. In addition, a region of one of the sequences of the dsRNA having a sequence length of from about 19 to about 23 nucleotides is complementary to a nucleotide sequence of the RNA produced from the target gene. | 01-07-2010 |
20100016574 | 2'-Hydroxyl Group-Modified Ribonucleoside Derivatives - Provided is a ribonucleoside derivative represented by General Formula (I): | 01-21-2010 |
20100029924 | Process for the Preparation of Poly(Alkoxylated) Oligonucleotides - A process for the preparation of a poly(alkoxylated) oligonucleotide is provided. The process comprises reacting an oligonucleotide which has been purified by ultrafiltration with a poly(alkoxide) thereby to form a poly(alkoxylated) oligonucleotide. The poly(alkoxyalted) oligonucleotide may be separated from non-poly(alkoxyalted) oligonucleotide by ultrafiltration under denaturing conditions, such as the presence of organic solvents, for example, ethanol; the presence of urea; the addition of chaotropic salts, for example perchlorate and guanidinium salts; the presence of formamide; and the application of heat, for example a temperature of up to about 70° C. | 02-04-2010 |
20100036108 | Universal Base - The present invention provides artificial universal base capable of base pairing nonspecifically with any of four kinds of natural nucleic acid bases (A, T, G, and C) without the function to specifically recognize pairing natural nucleic acid bases for base pair formation. | 02-11-2010 |
20100099860 | CAPTURE MOLECULES FOR THE DETECTION OF AMPLICONS WITH HIGH SENSITIVITY - The invention relates to a method for the design and/or the preparation of a polynucleotide capture molecule for detecting an amplicon having one strand serving as target to be detected and/or quantified after hybridization on said capture molecule, comprising the steps of: (a) selecting a primer pair defining the amplicon; (b) selecting a specific sequence of 10 to 100 nucleotides within the amplicon, such that said specific sequence defines two non-complementary ends of the amplicon; (c) defining the capture molecule having a capture portion that is complementary to the specific sequence selected in step b), and a spacer portion comprising at least 20 nucleotides; and (d) identifying among the two non-complementary ends of the amplicon a spacer end and a non-spacer end, respectively, such that the spacer end is non-complementary to the spacer portion of the capture molecule, and said spacer end exceeds said non-spacer end by at least 50 bases. | 04-22-2010 |
20100137572 | OXIDATION PROCESS - A process for the preparation of an oligonucleotide having at least one phosphate internucleotide linkage I provided. The process comprises the steps of:
| 06-03-2010 |
20100137573 | SYNTHESIS OF SELENIUM-DERIVATIZED NUCLEOSIDES, NUCLEOTIDES, PHOSPHORAMIDITES, TRIPHOSPHATES AND NUCLEIC ACIDS - The present invention provides selenium derivatives of nucleosides, nucleoside phosphoramidites, nucleotides, nucleotide triphosphates, oligonucleotides, polynucleotides, and larger nucleic acids and methods for their synthesis. Selenium derivatives of both ribonucleic acids and deoxyribonucleic acids, as well as methods for their synthesis, crystallization and uses in structural determinations, particularly by X-ray crystallographic techniques are disclosed. The selenium derivatives of the present invention are also useful as food supplements. | 06-03-2010 |
20100216983 | SYNTHESIS OF LOCKED NUCLEIC ACID DERIVATIVES - The invention relates to a novel strategy for the synthesis of Locked Nucleic Acid derivatives, such as α- | 08-26-2010 |
20100324278 | RNA synthesis-phosphoramidites for synthetic RNA in the reverse direction, and application in convenient introduction of ligands, chromophores and modifications of synthetic RNA at the 3'-end - The present invention relates to novel phosphoramidites, A-n-bz, C-n-bz, C-n-ac, G-n-ac and U are produced with an HPLC purity of greater than 98% and | 12-23-2010 |
20110092690 | LINKER AND SUPPORT FOR SOLID PHASE SYNTHESIS OF NUCLEIC ACID - The invention provides a universal linker capable of synthesizing nucleic acid having a phosphate group at the 3′ terminal, a universal support carrying the linker, and a synthesis method of nucleic acid using the universal support. The linker for solid phase synthesis of nucleic acid contains a compound represented by at least one of the following formulae | 04-21-2011 |
20110112285 | NOVEL METHOD FOR PREPARING OLIGONUCLEOTIDES COMPRISING A 5-PHOSPHATE MONOESTER OR 5-THIOPHOSPHATE MONOESTER END - The preparation of oligonucleotides having a 5′-phosphate monoester end. | 05-12-2011 |
20110144319 | Modified Oligonucleotides and Applications Thereof - Disclosed, among other things, are primers containing certain modified nucleobases in the 3′ terminal region of the primers that provide reduced formation of primer-dimers during amplification reactions, and various methods of use thereof. | 06-16-2011 |
20110178284 | Novel protective group for synthesis of RNA and derivative - A protective group represented by the following general formula (I) (the oxygen atom attached with * represents oxygen atom of 2′-hydroxyl group of a ribonucleoside, a ribonucleotide or a derivative thereof; R | 07-21-2011 |
20110178285 | METHODS AND COMPOSITIONS FOR PROCESSING CHEMICAL REACTIONS - Disclosed herein are compositions, methods and systems for the processing of chemical reactions, such as the synthesis of polymers. | 07-21-2011 |
20110196144 | SYNTHESIS AND USE OF 2'-SUBSTITUTED-N6-MODIFIED NUCLEOSIDES - An improved method of preparing a sugar modified nucleoside analog includes a protocol in which a hydroxy group of a sugar is selectively deprotected and oxidized prior to nucleophilic modification of the corresponding carbonyl group. The modified sugar is then coupled to a heterocyclic base that is modified with a dual nucleophilic reagent in a further step that provides N6-modified adenosine analogs with high stereoselectivity. | 08-11-2011 |
20110196145 | PROCESS FOR DESILYLATION OF OLIGONUCLEOTIDES - The present invention relates to processes and reagents for oligonucleotide synthesis and purification. One aspect of the present invention relates to compounds useful for activating phosphoramidites in oligonucleotide synthesis. Another aspect of the present invention relates to a method of preparing oligonucleotides via the phosphoramidite method using an activator of the invention. Another aspect of the present invention relates to sulfur-transfer agents. In a preferred embodiment, the sulfur-transfer agent is a 3-amino-1,2,4-dithiazolidine-5-one. Another aspect of the present invention relates to a method of preparing a phosphorothioate by treating a phosphite with a sulfur-transfer reagent of the invention. In a preferred embodiment, the sulfur-transfer agent is a 3-amino-1,2,4-dithiazolidine-5-one. Another aspect of the present invention relates to compounds that scavenge acrylonitrile produced during the deprotection of phosphate groups bearing ethylnitrile protecting groups. In a preferred embodiment, the acrylonitrile scavenger is a polymer-bound thiol. Another aspect of the present invention relates to agents used to oxidize a phosphite to a phosphate. In a preferred embodiment, the oxidizing agent is sodium chlorite, chloroamine, or pyridine-N-oxide. Another aspect of the present invention relates to methods of purifying an oligonucleotide by annealing a first single-stranded oligonucleotide and second single-stranded oligonucleotide to form a double-stranded oligonucleotide; and subjecting the double-stranded oligonucleotide to chromatographic purification. In a preferred embodiment, the chromatographic purification is high-performance liquid chromatography. | 08-11-2011 |
20110207920 | 5-BROMO-2'-DEOXY-URIDINE LABELED NUCLEOTIDE TRIPHOSPHATES AND NUCLEIC ACID PROBES AND METHODS OF MAKING AND USING THE SAME - 5-bromo-2′-deoxy-uridine (BrdU) labeled nucleotide triphosphates and nucleic acid probes are described herein. The BrdU labeled nucleotide triphosphates include a linker between the nucleotide triphosphate and the BrdU moiety. The linker can be cleavable or non-cleavable. The nucleotide triphosphates can be a ribonucleotide triphosphates, 2′-deoxyribonucleotide triphosphates or 2′,3′-dideoxyribonucleotide triphosphates. The nucleic acid probes can be used for in situ hybridization. | 08-25-2011 |
20120029181 | SYNTHESIS OF 5-AZACYTIDINE - The present invention provides a method for the preparation of 5-azacytidine, wherein 5-azacytidine is represented by the structure: | 02-02-2012 |
20120277419 | METHOD FOR MANUFACTURING PEGYLATED OLIGONUCLEOTIDES - A method for preparing a therapeutic pegylated oligonucleotide is described. The method involves synthesis, cleavage and purification steps designed to improve the efficiency whereby the therapeutic oligonucleotide may be prepared. Also described are methods for large scale preparation at the improved efficiency. | 11-01-2012 |
20130072672 | Method for F-18 FLT Synthesis - The present invention establishes a fast and simple [F-18] FLT synthesis process. Solid extraction units are used for purification to achieve an equally high and constant radiochemical yield and purity in a short period of time. By using a separation method, the impurities are reduced successfully while the total synthesis time is shortened. The radiochemical purity and the corrected radiochemical yield are both high. | 03-21-2013 |
20130085271 | POST-SYNTHETIC MODIFICATION OF NUCLEIC ACIDS BY INVERSE DIELS-ALDER REACTION - The present invention concerns a method and a kit for the post-synthetic modification of nucleic acids via an inverse Diels-Alder reaction. | 04-04-2013 |
20130090466 | CRYSTALLIZATION PROCESS OF CYCLIC ADENOSINE 3',5'-MONOPHOSPHATE - Provided is a crystallization process of cyclic adenosine 3′,5′-monophosphate, which comprises the following steps: 1) reacting an aqueous solution of cyclic adenosine 3′,5′-monophosphate with a base to obtain a salt of cyclic adenosine 3′,5′-monophosphate; 2) reacting the cyclic adenosine 3′,5′-monophosphate salt solution obtained in step 1) with an acid to obtain cyclic adenosine 3′,5′-monophosphate; 3) keeping cyclic adenosine 3′,5′-monophosphate obtained in step 2) at 0-15° C. | 04-11-2013 |
20130150570 | NUCLEASE RESISTANT DOUBLE-STRANDED RIBONUCLEIC ACID - This invention relates to modified double-stranded oligoribonucleic acid (dsRNA) having improved stability in cells and biological fluids, and methods of making and identifying dsRNA having improved stability, and of using the dsRNA to inhibit the expression or function of a target gene. | 06-13-2013 |
20140031538 | Systems, methods, and a kit for determining the presence of fluids - This invention relates to nucleic-acid based product authentication and identification by determining authentication codes comprising target nucleic acids using oligonucleotide probes associated with samples. The presence of the authentication code is determined using detection methods, such as flow cytometric methods, capable of particle discrimination based on the light scattering or fluorescence properties of the particle. Target-correlated fluorescence signal, originating from a target nucleic acid hybridized to labeled complementary oligonucleotides is determined as an indicator of the presence of the authentication code. In some embodiments, an intercalating dye is used to determine the presence of target nucleotide/oligonucleotide heterodimers and identify an authentication code. | 01-30-2014 |
20140051845 | Method Involving 1-Benzotriazolyl Carbonate Esters of Poly(ethylene glycol) - The invention provides for preparing a polymer-active agent conjugate, the method comprising the steps of reacting an amino acid derivative with a biologically active agent under conditions to form a polymer-active agent conjugate. | 02-20-2014 |
20140107331 | PHOTORESPONSIVE NUCLEIC ACID MANUFACTURING METHOD - The present invention provides a manufacturing method that can easily manufacture a compound known as photoresponsive (photocoupling) nucleic acids at high yield in a shorter period of time than that of the conventional technology. The present invention relates to a method of manufacturing a photoresponsive nucleic acid which includes a step of reacting a nucleic acid having groups represented by the Formula I, the Formula III, the Formula IV, or the Formula V and a compound represented by the Formula II, or reacting a nucleic acid having groups represented by the Formula VI, the Formula VIII, the Formula IX, or the Formula X and a compound represented by the Formula VII by heating them by microwaves in the presence of a metal catalyst, a basic substance, and a solvent. | 04-17-2014 |
20140316126 | RNA MOLECULES AND USES THEREOF - The invention relates to a method of designing a short RNA molecule to increase the expression of a target gene in a cell through the down-regulation of a non-coding RNA transcript, said method comprising the steps of: a) obtaining the nucleotide sequence of the coding strand of the target gene, at least between 200 nucleotides upstream of the gene's transcription start site and 200 nucleotides downstream of the gene's transcription start site; b) determining the reverse complementary RNA sequence to the nucleotide sequence determined in step a); and c) designing a short RNA molecule which is the reverse complement or has at least 80% sequence identity with the reverse complement of a region of the sequence determined in step b); wherein said method does not include a step in which the existence of said non-coding RNA transcript is determined; and to such short RNA molecules and uses thereof. | 10-23-2014 |
20150011746 | Protected Linker Compounds - The invention features protected linker compounds which comprise at one terminus a protected amino group and at another other terminus a phosphorous activating group, such as a phosphoramidite group. These protected linker compounds are introduced chemically at the 5′-end of oligonucleotides for the purpose of preparing 5′-amino modified oligonucleotides. After deprotection, the thereby introduced amino group then allows further modification (e.g. attachment of dyes) or immobilization (on surfaces or beads) of the oligonucleotide. Specifically, the presented amino protecting group is designed to provide such protected linker compounds in a solid form, which facilitates efficient purification by precipitation or crystallization and aliquoting for distribution and storage. | 01-08-2015 |
20150087820 | MICROFLUIDIC REACTORS FOR OLIGONUCLEOTIDE SYNTHESIS - The present disclosure generally pertains to systems and methods for the chemical synthesis of micro-quantities of oligonucleotides or other chemical molecules. The system includes a reusable glass micro-reactor containing a paramagnetic solid support, a magnet, an electronic drive controller and an optical spectroscopy system capable of driving a plurality individual reactors. The system utilizes the electroosmotic movement of reactants through microfluidic channels. Spectrophotometric monitoring of the reaction products allows for the real-time determination of synthesis yield. | 03-26-2015 |
20150133648 | HYBRID SOLID SUPPORTS USEFUL FOR OLIGONUCLEOTIDE PRODUCTION - A method for preparing a crosslinked polymer coated controlled porosity glass (CPG) particle is provided. The method involves mixing CPG particles in a solution comprising polyvinylbenzylchloride and a first solvent at a temperature below 10° C. A second solvent is added and a crosslinking agent is added to the mixture. The first solvent is removed rapidly within hours of addition of the crosslinking agent. The crosslinking reaction is permitted to proceed and the mixture is then cooled and treated to remove any remaining solvent. The resulting coated CPG particles are washed and dried. Also provided a polymer coated CPG particles using for loading ligand thereon. | 05-14-2015 |
20150376602 | Compositions and Methods for Multiplex Nucleic Acids Synthesis - Aspects of the invention relate to methods, compositions for designing and producing a target nucleic acid. In particular, aspects of the invention relate to the multiplex synthesis of target polynucleotides. | 12-31-2015 |