| Class / Patent application number | Description | Number of patent applications / Date published |
|---|
| 530415000 | Selective absorbtion, e.g., Ca phosphate sorbents, etc. | 31 |
| 20120232254 | Removal of Endotoxin Using Amphiphilic Core-shell Nanosorbents - Method for removal of endotoxin from protein preparations using core-shell nanoparticles, which have the ability to selectively adsorb endotoxin molecules in a protein mixture. The method comprises the steps of (a) preparing a plurality of core-shell nanoparticles; (b) adding the core-shell nanoparticles into a protein preparation containing endotoxin; (c) incubating the core-shell nanoparticles with the protein preparation for a period of time; and (d) separating nanoparticles from the protein preparation. | 09-13-2012 |
| 20090082552 | Microfluidic protein assay - A microfluidic device for separating a fluid sample into components is disclosed. The microfluidic device is used for assaying proteins for the purpose of identification of protein species. The microfluidic device employs integrated features such as a sample well for holding a protein sample, a thermal control device for heating the protein sample in the sample well, and a protein separation region in fluid communication with the sample well. Also disclosed is a method for assaying a protein sample. | 03-26-2009 |
| 20080262207 | Benzimidazole compounds and their use as chromatographic ligands - The present invention provides a new method for isolation and/or purification of immunoglobulins from a solution containing one or more immunoglobulins using a solid phase matrix represented by the formula: M-SP-L, wherein M is designates a matrix backbone, SP designates a spacer and L designates a substituted benzimidazole ligand. | 10-23-2008 |
| 20080293925 | Affinity Adsorbents for Plasminogen - For the separation, removal, isolation, purification, characterisation, identification or quantification of plasminogen or a protein that is a plasminogen analogue, an affinity adsorbent is used that is a compound of formula (II) wherein one X is N and the other is N, C—Cl or C—CN; A is a support matrix, optionally linked to the triazine ring by a spacer; Z is O, S or N—R and R is H, C | 11-27-2008 |
| 20090215997 | SEPARATION METHOD - A method of separating a fluorescent protein from a sample containing a plurality of proteins containing the fluorescent protein is provided. The method comprising: preparing a sample solution by adding the sample to a liquid; preparing an adsorption apparatus having a filling space for filling an adsorbent having a surface, wherein at least the surface of the adsorbent is constituted of a calcium phosphate-based compound and at least a part of the filling space is filled with the adsorbent; supplying the sample solution into the filling space of the adsorption apparatus so that the plurality of proteins are adsorbed by the adsorbent; supplying a phosphate elution buffer for eluting the fluorescent protein contained in the plurality of proteins from the adsorbent into the filling space of the adsorption apparatus to thereby obtain an eluant containing the fluorescent protein; and fractionating the eluant which is discharged from the filling space of the adsorption apparatus into a portion of the phosphate elution buffer containing the fluorescent protein and other portions thereof to thereby separate the fluorescent protein from the plurality of proteins. According to the present invention, it is possible to separate a large amount of the fluorescent protein from the sample containing the plurality of proteins containing the fluorescent protein with high purity by a simple operation. | 08-27-2009 |
| 20110098453 | MAGNETIC NANOCOMPOSITE, AND PROCESS FOR SELECTIVE BINDING, SEPARATION AND PURIFICATION OF PROTEIN USING THE SAME - The present invention relates to a magnetic nanocomposite, a process for production thereof, a reusable protein-binding agent for separation of a protein including the magnetic nanocomposite, and a process for selective binding, separation and purification of a protein using the magnetic nanocomposite. In particular, the present invention is directed to a magnetic nanocomposite with a magnetic nanoparticle core of a magnetic nanoparticle, a silica shell coating said core, and a nanoparticle layer of a fourth period transition metal oxide, which coats said silica shell, a process for production of the magnetic nanocomposite, a reusable protein-binding agent the magnetic nanocomposite, and a process for selective binding, separation and purification of a protein using the magnetic nanocomposite. | 04-28-2011 |
| 20080214794 | Method and device for extracting an analyte - The invention provides columns and methods for the purification and concentration of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution. The columns typically include a bed of extraction medium positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. The invention also provides methods for purifying and concentrating multiple analytes simultaneously. | 09-04-2008 |
| 20100305311 | NANOPARTICLE FOR SEPARATING PEPTIDE, METHOD FOR PREPARING THE SAME, AND METHOD FOR SEPARATING PEPTIDE USING THE SAME, - Disclosed are nanoparticles for use in the isolation of peptides, a method for producing the nanoparticles, and a method for the isolation of peptides using the nanoparticles. The nanoparticles comprise magnetic nanoparticles and thiol-specific functional groups as first functional groups bound to the surfaces of the magnetic nanoparticles to selectively capture cysteine-containing peptides. The nanoparticles allow highly selective isolation of target peptides in a simple and rapid manner. Therefore, the nanoparticles can be applied to research on the treatment of diseases such as cancers. | 12-02-2010 |
| 20120157668 | METHODS FOR REMOVAL OF MICROCYSTINS AND ISOLATION OF PHYCOCYANIN FROM CYANOBACTERIA - This disclosure relates to methods of removing contaminating microcystins toxins from preparations of blue-green algae. It also relates to methods of purifying phycocyanin from blue-green algae extracts. | 06-21-2012 |
| 20110092680 | METHOD OF SEPARATING AND PURIFYING CELLULAR COMPONENTS USING NON-COVALENT BOND BETWEEN CUCURBITAL DERIVATIVE AND GUEST COMPOUND AND APPARATUS USING THE SAME - Provided is a method of separating cellular components, the method including: a) contacting a guest compound-bound reactive compound with cells; b) lysing the cells; c) adding a host compound-bound solid phase to a solution including the lysates of the cells to prepare a mixture; d) separating binding pairs of the guest compound bound to cellular components and the host compound bound to the solid phase from the mixture, and purifying the binding pairs; and e) separating the cellular components from the binding pairs, wherein the guest compound-bound reactive compound is obtained through a covalent bond between a reactive compound and a guest compound represented by Formula 2 below guest, the host compound-bound solid phase is obtained through a covalent bond between a solid phase and a host compound represented by Formula 1 below, and the reactive compound includes at least one selected from the group consisting of a biomolecule, N-hydroxysuccimide, an antigen, an antibody, an aptamer, folic acid, transferrin, and any mixtures thereof. | 04-21-2011 |
| 20110184155 | CARRIER POLYMER PARTICLE, PROCESS FOR PRODUCING THE SAME, MAGNETIC PARTICLE FOR SPECIFIC TRAPPING, AND PROCESS FOR PRODUCING THE SAME - Carrier polymer particles comprising organic polymer particles having a particle diameter of 0.1 to 20 micrometers and a saccharide with which the surface of the organic polymer particles is covered, the organic polymer particles and the saccharide being chemically bonded. | 07-28-2011 |
| 530416000 | Ion exchange | 14 |
| 20130035477 | Method for Removing Endotoxin from Proteins - Disclosed is a method for removing endotoxin from proteins. Also disclosed are products made by using the method. The method may be used, for example, to produce endotoxin-free lactoferrin. Bovine milk-derived lactoferrin may be produced in commercial quantities by the method, and endotoxin-free bovine lactoferrin may be used for a variety of therapeutic uses, including improving wound healing. | 02-07-2013 |
| 20090306352 | Basic Protein Purification Tags from Thermophilic Bacteria - The invention is related to a method for purification of recombinant proteins using highly basic proteins from thermophilic bacteria as purification tags for use in a cation-exchange chromatography purification step. The basic proteins may be ribosomal proteins. The recombinant proteins are expressed in eukaryotic or prokaryotic host cells. The purification tag will typically have a pl above about 9 and comprise from about 15 to about 250 amino acid residues. | 12-10-2009 |
| 20090318674 | PROCESS FOR PURIFICATION OF ANTIBODIES - The disclosed embodiments are directed to methods and compositions for purification of proteins, in particular, to methods and compositions for an antibody purification process that includes aggregate removal and the use of solubility enhancing additives such as zwitterion-containing compositions to enhance antibody solubility and avoid aggregate formation or occlusion during ion exchange chromatography, yielding a high-purity protein product substantially free of aggregates. | 12-24-2009 |
| 20090036655 | Production of 2S Canola Protein Involving Ion Exchange - Substantially pure 2S canola protein is obtained substantially free from 7S and 12S canola protein by a procedure in which 2S canola protein is captured by binding to a cation-exchange medium while permitting other proteins and impurities to be washed away. The 2S canola protein then is removed from the cation-exchange medium by exposure of the cation-exchange medium to saline at a suitably high salt concentration. | 02-05-2009 |
| 20080269469 | Method of Separating Protein - It is intended to provide a method whereby a protein can be separated and purified at a high accuracy by a convenient procedure. A sample containing the desired protein is brought into contact with an ion exchanger under first conditions at a high ionic strength and at pH value not in the vicinity of the isoelectric point of the desired protein. Next, the component adsorbed by the ion exchanger is eluted under second conditions at a lower ionic strength than in the first conditions and at a pH value closer to the isoelectric point of the desired protein than in the first conditions. | 10-30-2008 |
| 20120077966 | POLYNUCLEOTIDE AND POLYPEPTIDE SEQUENCE AND METHODS THEREOF - The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably | 03-29-2012 |
| 20090105465 | Protein Purification Using HCIC and Ion Exchange Chromatography - The present invention provides methods for purifying proteins. In particular, the methods employ a two-step non-affinity chromatography process without the use of an in-process tangential flow filtration step. | 04-23-2009 |
| 20110118453 | PROTEIN SEPARATION VIA ION-EXCHANGE CHROMATOGRAPHY AND ASSOCIATED METHODS, SYSTEMS, AND DEVICES - The present invention provides methods for separating proteins from a protein mixture. In one aspect, a method for separating a high concentration protein mixture into a bound protein fraction and a flow-through protein fraction can include delivering a protein mixture through an ion exchange column at a fixed pH and a fixed salt concentration. The fixed pH and the fixed salt concentration have been preselected to cause separation of the protein mixture into a bound protein fraction and a flow-through protein fraction. In this case, the bound protein fraction binds to the ion exchange column and the flow-though protein fraction flows though the ion exchange column. The method can further include receiving the flow-through protein fraction from the ion exchange column separate from the bound protein fraction, wherein either the bound protein fraction or the flow-through fraction contains a protein of interest. | 05-19-2011 |
| 20110166332 | ENHANCED ANTIBODY AGGREGATE REMOVAL WITH CAPTO ADHERE IN THE PRESENCE OF PROTEIN-EXCLUDED ZWITTERIONS - The invention relates to a method for separating at least one non-aggregated protein from a liquid protein preparation by contacting said preparation with a multimodal anion exchanger in the presence of protein-excluded zwitterions at a concentration of 0.25 to 2.5 M. | 07-07-2011 |
| 20110092682 | Methods and Systems for Purifying Non-Complexed Botulinum Neurotoxin - Methods and systems for chromatographically purifying a | 04-21-2011 |
| 20110092681 | Purification of VWF for Increased Removal of Non-Lipid Enveloped Viruses - The present invention provides methods for purifying Von Willebrand factor (VWF) for increased removal of non-lipid enveloped viruses. | 04-21-2011 |
| 20090043080 | Purification of a Bulk of a Factor VII Polypeptide by Fractionated Elution from an Anion-Exchange Material - The present invention relates to the purification of a Factor VII polypeptide from a bulk of a Factor VII polypeptide with respect to desirable glycoforms by fractionated elution from an anion-exchange material with an eluting buffer comprising a certain concentration of calcium ions. The present invention renders it possible to enrich a bulk of a Factor VII polypeptide with respect to desirable glycoforms. | 02-12-2009 |
| 20090118476 | Purification of pegylated polypeptides - The invention is a method for the purification of mono-PEGylated erythropoietin using two cation exchange chromatography steps wherein the same type of cation exchange material is used in both cation exchange chromatography steps and a method for producing a mono-PEGylated erythropoietin in substantially homogeneous form. | 05-07-2009 |
| 20100292447 | METHOD AND AGENT FOR REFOLDING PROTEINS - This invention relates to the use of ionic liquids comprising a cation with at least one electron donor region and one positively charged electrostatic region which are spatially distinct from each other for protein refolding and a method for refolding proteins using said ionic liquids. | 11-18-2010 |
| 530417000 | Chromatography or by septum selective as to material, e.g., gel filtration, molecular sieve dialysis, etc. | 6 |
| 20120202978 | CHROMATOGRAPHY EQUIPMENT CHARACTERIZATION - Herein is reported a method for determining whether a re-useable chromatography column packing, which is used at least for the second time in a purification step of a purification of a polypeptide, has reduced separation efficacy in said purification step of said purification of said polypeptide, comprising the following steps: a) identifying and determining the experimental data of an inert change of at least one physicochemical parameter of a mobile phase passing through said re-useable chromatography column packing, b) determining the parameters of a function of formula I by fitting the experimental data of the inert change of the physicochemical parameter of the at least second use, c) determining the difference between the experimental data of the inert change of the physicochemical parameter of the at least second use and the function of formula I with the parameters determined in step b), d) calculating the difference between the maximum value and the minimum value of the difference determined in step c) and normalizing said difference, e) determining reduced separation efficacy of said re-useable chromatography column packing when the absolute value of the difference calculated in step d) is more than 0.1. | 08-09-2012 |
| 20090281288 | MATRIX FOR SEPARATION OF POLYETHERS AND METHOD OF SEPARATION - The present invention relates to a separation matrix comprised of a support to the surfaces of which polymer chains have been coupled, wherein each polymer chain presents recurring proton-donating groups and at least the surface of the support is substantially hydrophilic. In the most advantageous embodiment, the support is porous cross-linked agarose, the polymers are poly(acrylic acid) and the proton-donating groups are carboxyl groups. The matrix is useful e.g. to remove PEG from pegylated and/or native compounds in a liquid. Accordingly, the invention also encompasses a method, such as a chromatographic method, wherein the separation matrix according to the invention is used, for example as a pre-treatment of a reaction mixture that comprises unreacted PEG, pegylated proteins and native proteins. | 11-12-2009 |
| 20120016113 | BUOYANT PROTEIN HARVESTING DEVICE - A buoyant device containing chromatography media performs the function of protein harvesting replacing the steps of cell separation and volume reduction; the device can be loaded into columns for further purification. | 01-19-2012 |
| 20120253024 | METHOD AND SYSTEM FOR POLYPEPTIDE PURIFICATION - The present invention provides a method and automated system for the purification of polypeptides including the direct filtration of solutions containing the polypeptides after purification. | 10-04-2012 |
| 20120232255 | PRETREATMENT CARTRIDGE FOR SEPARATING SUBSTANCE AND METHOD OF PRETREATMENT USING THE SAME - Pretreatment is performed using a pretreatment cartridge for separating substances that is packed with a stationary phase that is coated on its surface with a polymer whose tendency to hydration will change within a temperature range of 0-80° C. and which is capable of changing the affinity between the substance to be separated and the surface of the stationary phase in response to the shrinkage or swelling of the polymer chain due to temperature change. By using this pretreatment cartridge for separating substances, a convenient operation will suffice for a sample solution to be reduced to a solution containing the substance that need be separated from the specimen. If this method of pretreatment is used, useful substances can be separated intact by merely changing the temperature and it becomes possible to perform the subsequent analyzing or fraction collecting operation efficiently. | 09-13-2012 |
| 20120271042 | METHOD FOR ISOLATION OF GENOMIC DNA, RNA AND PROTEINS FROM A SINGLE SAMPLE - The invention provides systems, methods and kits for the separation and/or purification of at least two cellular components selected from genomic DNA, RNA and proteins. The method includes first lysing a biological sample to generate an aqueous solution containing the cellular components; then applying the aqueous solution to a first mineral support under conditions for genomic DNA to bind; and collecting the flowthrough which contains unbound total RNA and proteins. The method further includes applying the flowthrough to a second mineral support under conditions for RNA to bind, and collecting the flowthrough which contains proteins. The genomic DNA and total RNA bound can be eluted while the protein in the flowthrough can be further purified. Further the total RNA isolated could be used to isolate small RNA such as microRNA. | 10-25-2012 |
