| Class / Patent application number | Description | Number of patent applications / Date published |
|---|
| 530413000 | Immunological separation or affinity chromatography | 39 |
| 20080200658 | Process For The Purification Of Il-18 Binding Protein - The invention relates to a process for the production of purified IL-18 binding protein (IL-18BP) from a fluid comprising affinity chromatography. | 08-21-2008 |
| 20090264631 | IMMUNOADSORPTION OF ANTI-VON WILLEBRAND FACTOR CLEAVING PROTEASE ANTIBODIES - This invention relates to adsorbents and methods for highly selective removal of anti-von Willebrand Factor-cleaving protease antibodies (“anti-vWF-cp-abs”) from human plasma using human von Willebrand Factor-cleaving protease (“hvWF-cp”) or fragments thereof as affinity ligands. The adsorbents can be used for treating disorders associated with the occurrence of anti-vWF-cp-abs in patients, such as thromboembolic diseases. | 10-22-2009 |
| 20090306351 | Wash Buffer And Method Of Using - A method for purifying a protein using Protein A chromatography comprising a) absorbing the protein to Protein A immobilized on a solid support; b) removing contaminants by washing the immobilized Protein A containing the absorbed protein with a buffer comprising one or more chaotropic agents in combination with one or more hydrophobic modifiers and having a pH of at least 7.0; and c) eluting the protein from the Protein A immobilized on the solid support. | 12-10-2009 |
| 20130096284 | METHOD FOR PURIFYING PROTEIN USING AMINO ACID - In large-scale purification of proteins such as antibodies, an economic high-purity purification method is required. The present invention relates to a method for purifying a protein, including one or more chromatographic processes, in which an amino acid; or a dipeptide, an oligopeptide, or a polyamino acid thereof is included in a buffer solution used in at least one chromatographic processes (equilibration buffer, wash buffer, and elution buffer), thereby purifying a high-purity protein with a very small quantity of the impurity (e.g., polymers or host cell proteins). | 04-18-2013 |
| 20100022760 | MUTANT PROTEIN - The present invention relates to an immunoglobulin-binding protein, wherein at least one asparagine residue has been mutated to an amino acid other than glutamine or aspartic acid, which mutation confers an increased chemical stability at pH-values of up to about 13-14 compared to the parental molecule. The protein can for example be derived from a protein capable of binding to other regions of the immunoglobulin molecule than the complementarity determining regions (CDR), such as protein A, and preferably the B-domain of Staphylococcal protein A. The invention also relates to a matrix for affinity separation, which comprises an immunoglobulin-binding protein as ligand coupled to a solid support, in which protein ligand at least one asparagine residue has been mutated to an amino acid other than glutamine. | 01-28-2010 |
| 20090099344 | REDUCING PROTEIN A LEACHING DURING PROTEIN A AFFINITY CHROMATOGRAPHY - A method for reducing leaching of protein A during protein A affinity chromatography is described which involves reducing temperature or pH of, or by adding one or more protease inhibitors to, a composition that is subjected to protein A affinity chromatography. | 04-16-2009 |
| 20100016564 | METHOD OF PREPARING AN IMMOBILISED METAL ION CHROMATOGRAPHY ADSORBENT AND METHODS OF PURIFYING PROTEINS, PEPTIDES OR POLYNUCLEOTIDES - The present invention relates to a method of preparing an immobilised metal ion affinity chromatography (IMAC) adsorbent, which comprises to provide chromatography ligands comprised of alkylene diamine triacetic acid, or a derivative thereof, and coupling thereof to a carrier via nitrogen. In an advantageous embodiment, the alkylene diamine triacetic acid is ethylene diaminetriacetic acid (ED3A). | 01-21-2010 |
| 20110301342 | APPARATUS AND PROCESS FOR PURIFICATION OF PROTEINS - The invention is directed to an apparatus and method for purifying a protein. The apparatus involves the use of a capture chromatography resin, a depth filter arranged after the capture chromatography resin, and a mixed-mode chromatography resin arranged after the depth filter. The method involves providing a sample containing the protein, processing the sample through a capture chromatography resin, a depth filter, and a mixed-mode chromatography resin. A membrane adsorber or monolith may be substituted for the mixed-mode chromatography column. | 12-08-2011 |
| 20100069617 | ENHANCED PROTEIN AGGREGATE REMOVAL BY MIXED MODE CHROMATOGRAPHY ON HYDROPHOBIC INTERACTION MEDIA IN THE PRESENCE OF PROTEIN-EXCLUDED ZWITTERIONS - This invention relates to methods for enhancing purification of proteins such as monoclonal antibodies by chromatography on carboxyl group-containing HIC supports in the presence of zwitterions that are excluded from protein surfaces. In certain embodiments, the invention may permit more effective separation of non-aggregated protein from aggregated protein. | 03-18-2010 |
| 20080207880 | Reduction of the Content of Protein Contaminents in Compositions Comprising a Vitamin K-Dependent Protein of Interest - The present invention relates to Vitamin K-dependent protein compositions having a very low, or negligible, content of protein contaminants. The present invention also relates to method applicable in the preparation of such Vitamin K-dependent protein compositions. Such methods may either be used alone or in sequential combination with the purpose of reducing the relative content of protein contaminants. The present invention is particularly relevant in the preparation of compositions of coagulation factors selected from Factor X polypeptides (FX/FXa), Factor IX polypeptides (FIX/FIXa), Factor VII polypeptides (FVII/FVIIa), and the anticoagulant Protein C, in particular Factor VII polypeptides. | 08-28-2008 |
| 20090143570 | METHOD FOR ISOLATION OF GENOMIC DNA, RNA AND PROTEINS FROM A SINGLE SAMPLE - The invention provides systems, methods and kits for the separation and/or purification of at least two cellular components selected from genomic DNA, RNA and proteins. The method includes first lysing a biological sample to generate an aqueous solution containing the cellular components; then applying the aqueous solution to a first mineral support under conditions for genomic DNA to bind; and collecting the flowthrough which contains unbound total RNA and proteins. The method further includes applying the flowthrough to a second mineral support under conditions for RNA to bind, and collecting the flowthrough which contains proteins. The genomic DNA and total RNA bound can be eluted while the protein in the flowthrough can be further purified. Further the total RNA isolated could be used to isolate small RNA such as microRNA. | 06-04-2009 |
| 20080293924 | Process For the Isolation and/or Purification of Proteins - The invention relates to the process for the isolation and/or purification of biologically active proteins, preferably TNF-alpha or TNF-alpha analogues. The process of the present invention results in the production of high yields of proteins, preferably TNF-alpha or TNF alpha analogues with a purity of greater than 98%. The described process is particularly suitable for the industrial production of proteins, preferably TNF-alpha or TNF-alpha analogues. | 11-27-2008 |
| 20080249289 | Manufacture of a Chromatography Matrix - The present invention relates to method of manufacturing a sulphonate-functionalized (S-functionalized) cation exchanger, which method comprises reacting olefinic groups with bisulphite in the presence of at least one amine oxide, such as N-methyl morpholine oxide (NMO). The olefinic groups of the carrier may be allyl groups; which may be provided on extenders such as polyhydroxyfunctional polymers. In one embodiment, the carrier is made of agarose with improved flow pressure properties. | 10-09-2008 |
| 20090264630 | METHOD OF SEPARATING MONOMERIC PROTEIN(S) - The present invention relates to a method of separating one or more monomeric proteins, such as monomeric antibodies, from a liquid. The method comprises providing a thiophilic aromatic chromatography matrix; contacting the liquid that comprises proteins with the matrix; and recovering at least one monomeric protein, such as a monomeric antibody, from the flow-through fraction. The proteins are advantageously monomeric antibodies. | 10-22-2009 |
| 20090062515 | METHOD FOR PURIFYING BIOACTIVE SUBSTANCES - A method for purifying bioactive substances includes the steps of: causing a bioactive substance having histidine units to contact media, each constituted by a substrate, ligands which are physically attached to the surface of the substrate, and Cu(II) or Fe(II) metal ions which are covalently bonded to the ligands; causing the bioactive substance to covalently bond with the metal ions via the histidine units; and washing the media with an amount of 1 nmol/L to 10 mmol/L imidazole derivative solution 60 times the volume of the media or greater. In the case that the metal ions are Cu(II), the bioactive substance which has covalently bonded with the Cu(II) via the histidine units are recovered by one of a 10 mmol/L to 1 mol/L imidazole derivative solution and a 0.5 mmol/L to 5 mol/L EDTA solution. | 03-05-2009 |
| 20090209735 | AFFINITY SEPARATION BY PARTITION ENHANCING MATERIAL CONJUGATED BIOMOLECULES IN AQUEOUS TWO-PHASE EXTRACTION SYSTEM - The present invention relates to a method for separating a material that has affinity to an antibody by using a protein-antibody conjugate with modified partition characteristics, more precisely a method for affinity separation to separate a material specifically binds to an antibody, in which an antibody is conjugated to a protein to modify partition characteristics of the protein-antibody conjugate. The method of the present invention can be effectively and widely used as a safe and efficient separation method for biomolecules since it takes advantages of safe aqueous two-phase extraction system and high selective molecular specific conjugation. | 08-20-2009 |
| 20090209736 | MULTICOLUMN SEQUENTIAL SEPARATION PROCESS - The subject of the invention is separation on a solid support by multi-column sequential selective retention in order to separate a product of interest from a solution containing such a product of interest, by passing this solution over a fixed chromatographic resin bed comprising at least three zones, liquid flow means being placed between adjacent zones and between the last zone and the first zone, this process comprising several sequences, each sequence comprising at least one step chosen from an absorption step, a rinsing step and a desorption step, carried out either simultaneously or not, each following sequence is carried out by the displacement of the fronts in the zones towards the downstream end substantially of the same increment before the periodic displacement of the points of introduction and of withdrawal, characterized in that the process includes a sub-sequence without injection of the charge. | 08-20-2009 |
| 20090209737 | USE OF DENATURING AGENTS DURING AFFINITY CAPTURE - A method for affinity capturing target molecules is disclosed, comprising obtaining a sample comprising a target molecule; and, in the presence of a denaturing agent; affinity capturing the target molecule with a single chain affinity molecule or antigen binding portion thereof that specifically binds the target molecule, wherein the affinity molecule or antigen binding portion thereof is immobilized on a support. | 08-20-2009 |
| 20090253900 | Processing of Peptides and Proteins - The invention provides novel methionine aminopeptidase enzymes and their use. | 10-08-2009 |
| 20100228010 | METHOD FOR PURIFYING PROTEIN - The present invention provides a method for purifying a protein to remove impurities from a mixture liquid containing a desired protein and the impurities, comprising the step of performing filtration using a porous membrane having a graft chain on a pore surface and an anion-exchange group fixed to the graft chain. | 09-09-2010 |
| 20100305310 | Multi-Media Affinity Column to Prevent Leaching of Ligands - In an affinity-type purification, ligands dissociated from a packed bed that would otherwise leach into the solution containing the species being purified are captured by a second ligand that resides in a porous barrier downstream from the packed bed, the second ligand exhibiting an affinity-type interaction with the dissociated first ligand with sufficient specificity to avoid the undesired retention by the second ligand of species from the liquid sample or source liquid other than the species sought to be purified in the affinity column. | 12-02-2010 |
| 20100331530 | METHOD FOR PURIFYING THERAPEUTIC PROTEINS BY MEANS OF MULTI-STAGE EXTRACTION - Method for purifying therapeutic proteins by multi-stage extractive distillation. | 12-30-2010 |
| 20110009604 | Method for Concentrating, Purifying and Removing Prion Protein - The present invention relates to a method for concentrating and/or purifying prion PrP | 01-13-2011 |
| 20110118452 | Open Channel Solid Phase Extraction Systems and Methods - The invention provides, inter alia, methods, devices and reagents for the preparation of native and non-denatured biomolecules using solid-phase extraction channels. The invention is particularly suited for the purification, concentration and/or analysis of protein analytes. The invention further provides, inter alia, methods, devices and reagents for the purification, concentration and/or analysis of multi-protein complexes. | 05-19-2011 |
| 20100056766 | PURIFICATION OF BIOLOGICAL CONJUGATES BY SIZE EXCLUSION CHROMATOGRAPHY - A method for separating a biological conjugate from an aggregate. The molecular weight of the biological conjugate ranges from about 10 kDa to about 1000 kDa. In one embodiment, the method comprises the steps of:
| 03-04-2010 |
| 20100179308 | Method and Device for Sample Preparation - The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media. | 07-15-2010 |
| 20090182131 | Methods for removing anti-MHC antibodies from a sample - The present invention relates generally to anti-MHC assay methodologies utilizing functionally active, recombinantly produced, and truncated individual soluble MHC trimolecular complexes that are linked to a substrate. The methods include reacting a sample with the substrate having the MHC trimolecular complex linked thereto, whereby antibodies specific for the at least one MHC trimolecular complex linked to the substrate are removed from the biological sample. | 07-16-2009 |
| 20090247735 | ENHANCED CAPACITY AND PURIFICATION OF PROTEIN BY MIXED MODE CHROMATOGRAPHY IN THE PRESENCE OF AQUEOUS-SOLUBLE NONIONIC ORGANIC POLYMERS - This invention relates to the use of mixed mode chromatography for purification of a protein from a mixture containing other materials, including fragmented or aggregated antibodies, host cell proteins, DNA, endotoxin, and/or virus. This invention further relates to the integration of such a method into a multi-step procedure with other fractionation methods for purification of antibodies or other proteins suitable for in vivo applications. | 10-01-2009 |
| 20100267939 | DEVICE AND METHODS FOR PREPARATION OF PEPTIDES AND PROTEINS SAMPLES FROM SOLUTION - Featured are devices, methods and kits for preparation of samples of peptides and proteins from a solution; more particularly to such devices and methods in which carbon nanotubes are utilized for sample preparation and/or purification of peptide and/or protein samples from a solution. A sample to be treated/processed can comprise proteins, peptides or any other molecule having an amine moiety that can be protonated under low pH, such as, but not limited to, pH 5. Such a sample also can contain contaminants such as salts, detergents, etc. that will be eliminated during the sample preparation/purification process of the present invention. The methods, devices and kits of the present invention advantageously provide for the concentration of the sample, removal of contaminants and ease of manipulation of small liquids without the concomitant loss of sample. | 10-21-2010 |
| 20100292446 | ISOLATION OF DNA, RNA AND PROTEIN FROM A SINGLE SAMPLE - The invention provides a method for the separation and purification of two or three cellular components selected from genomic DNA, RNA and proteins from a single biological sample. The method comprises generating an aqueous solution containing the cellular components by lysing cells with a lysis solution; contacting the aqueous solution with an ion exchanger for genomic DNA and RNA to bind to the ion exchanger; collecting the flow-through which contains unbound proteins; eluting RNA from the ion exchanger; and eluting DNA from the ion exchanger. For the purification of any two of the cellular components, one of the components is not collected. The invention also provides reagent kits for carrying out the methods. | 11-18-2010 |
| 20080312425 | Ion Exchange Chromatography and Purification of Antibodies - A novel method for purifying antibody and other product protein concomitant with removing aggregates made up from single product protein species is devised. | 12-18-2008 |
| 20090048433 | Scalable Process for Protein Purification - The invention provides a process for the purification recombinantly expressed, self-assembled VLP from the homogenate of a bacterial host, wherein the process can be scaled up to a commercial production scale in a cost effective manner. The process comprises a first chromatography using an anion exchange matrix, a second chromatography using hydroxyapatite and, optionally, a size exclusion chromatography. VLP preparations obtained by the process of the invention are essentially free of endotoxin contaminations. | 02-19-2009 |
| 20090259029 | [1, 2, 4] TRIAZOLO [1, 5-A] PYRIMIDINE DERIVATIVES AS CHROMATOGRAPHIC ADSORBENT FOR THE SELECTIVE ADSORPTION OF IGG - The present invention relates to a chromatographic adsorbent for selectively adsorbing IgG, comprising the following formula | 10-15-2009 |
| 20120029176 | Chromatography Media And Method - Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs). | 02-02-2012 |
| 20110105736 | SEPARATING AGENT FOR PROTEIN PURIFICATION AND PROTEIN PURIFICATION METHOD - A novel separating agent for protein purification which not only can adsorb proteins in a sufficient amount for protein purification from a low concentration buffer but also can desorb the adsorbed protein easily just by altering the pH of the buffer and a simple and economical method for its production and a method for protein purification using it. | 05-05-2011 |
| 20120149885 | DOWNSTREAM BIOPROCESSING DEVICE - Large-scale downstream processing of secreted recombinant proteins is provided in a single device, wherein the contents of a plurality of bioreactors are combined simultaneous to their harvesting and purification resulting in significant savings of time and the cost of manufacturing. | 06-14-2012 |
| 20120214976 | Scalable Process for Protein Purification - The invention provides a process for the purification recombinantly expressed, self-assembled VLP from the homogenate of a bacterial host, wherein the process can be scaled up to a commercial production scale in a cost effective manner. The process comprises a first chromatography using an anion exchange matrix, a second chromatography using hydroxyapatite and, optionally, a size exclusion chromatography. VLP preparations obtained by the process of the invention are essentially free of endotoxin contaminations. | 08-23-2012 |
| 20100048880 | Starch Binding Domain and Use Thereof - The present invention relates to a starch binding domain, a recombinant protein and a complex thereof. The present invention also relates to a method for separating a recombinant protein comprising a starch binding domain of the present invention. | 02-25-2010 |
| 20100292445 | Method for Efficient Purification of Bionanocapsule - The present invention provides a method for purifying a heat-resistant bio-nanocapsule composed mainly of a protein produced by using a eukaryotic cell. The method comprises heat-treating a lysate of the eukaryotic cell to remove contaminant proteins, and then subjecting the resulting product to chromatography. | 11-18-2010 |
