Entries |
Document | Title | Date |
20080227662 | Livestock tissue identification system - A system receives tissue samples and identification information of sources of tissue samples in an array of at least two and preferably at least four containers having one open end. A support secures the array of containers together. The open end of each container faces in a same direction. The array contains unique identification information for each of the at least two or at least four containers. The unique identification information is machine readable. At least two or at least four open end caps securely close the open end. | 09-18-2008 |
20080227663 | SYSTEMS AND METHODS FOR HIGH SPEED ARRAY PRINTING AND HYBRIDIZATION - Novel and improved systems and methods for high speed arraying, hybridization, quantitative development and/or assaying are provided. Some embodiments provide a web based arraying format. Some other embodiments provide a sheet based arraying format. Some embodiments use a drop on drop assaying or hybridization mode. In some embodiments, a substantially inert substrate is utilized. In some other embodiments, an interactive substrate is utilized. | 09-18-2008 |
20080227664 | CELL ARRAY STRUCTURAL BODY AND CELL ARRAY - The present invention provides a cell array structural body containing a substrate, a plurality of micropores piercing the substrate from one surface to another surface, through which a sample cell can pass, and a capture/release unit for the sample cell on a wall surface of each micropore, as well as a cell array with such structural body detachably immobilizing sample cells in its micropores. In using the cell array structural body and the cell array for drug evaluations or the like, handling of a culture medium or a drug solution, or washing procedure is easy, and harvest of a desired cell from the cell array is easy and trustworthy. | 09-18-2008 |
20080234144 | Apparatus and method for digital magnetic beads analysis - A micro bead having a digitally coded structure that is partially transmissive and opaque to light. The pattern of transmitted light is determined by to decode the bead. The coded bead may be structured a series of alternating light transmissive and opaque sections, with relative positions, widths and spacing resembling a 1D or 2D bar code image. To decode the image, the alternating transmissive and opaque sections of the body are scanned in analogous fashion to bar code scanning. The coded bead may be coated or immobilized with a capture or probe to effect a desired bioassay. The coded bead may include a paramagnetic material. A bioanalysis system conducts high throughput bioanalysis using the coded bead, including a reaction detection zone and a decoding zone. Alternative method for barcode determination is based on barcode pattern image processes. Microbeads can also be settled down to the bottom of the well in a microplate, so the barcode can be decoded by image processed directly. Therefore, multiple-analyte tests can be performed in one single well. Microplate is a standard format; each plate can have 96, 384, or 1536 patient samples. The bead image is taken with a conventional microscope and optical detector from the bottom of an optically clear microplate, and barcode pattern can be decoded by image software. Therefore, the whole bead experiment can be performed in the microplate without taking the beads out. | 09-25-2008 |
20080242562 | APPARATUS AND METHODS FOR DETECTING NUCLEIC ACID IN BIOLOGICAL SAMPLES - There are disclosed apparatus and methods for the field-assisted acceleration of biological processes involving charged entities, including in particular the detection of target DNA in a biological sample. A reaction cell is provided with a dielectric surface, and a field is generated by inducing charge-separation in the dielectric material by applying a potential to an electrode in contact with the dielectric material. | 10-02-2008 |
20080255006 | MAGNETIC NANOPARTICLES, MAGNETIC DETECTOR ARRAYS, AND METHODS FOR THIER USE IN DETECTING BIOLOGICAL MOLECULES - Magnetic nanoparticles and methods for their use in detecting biological molecules are disclosed. The magnetic nanoparticles can be attached to nucleic acid molecules, which are then captured by a complementary sequence attached to a detector, such as a spin valve detector or a magnetic tunnel junction detector. The detection of the bound magnetic nanoparticle can be achieved with high specificity and sensitivity. | 10-16-2008 |
20080255007 | Substrate Material For Handling and Analyzing Samples - The invention relates to a substrate material for analyzing one or more fluid samples for the presence, amount or identity of one or more analytes in the samples, whereby the substrate material is adapted in that way that a flow of the sample or parts thereof in and/or with the substrate material is influenced and/or caused by phase transitions, preferably photoinducable phase transitions, in selected areas of the substrate material. | 10-16-2008 |
20080255008 | GELATION CONTROLLED FLUID FLOW IN A MICROSCALE DEVICE - A method of self regulating a process of manufacturing a biological device which includes the steps of: choosing a first material and a second material based on a correlation of a parameter of the second material with a parameter of the first material; and merging the first material with the second material where the correlation of the parameter of the second material with the parameter of the first material self regulates the merging step to provide a distinct patterning of the first material and the second material. | 10-16-2008 |
20080269078 | Nanoscale array biomolecular bond enhancer device - The invention teaches the use of an addressable nanoscale device to create a programmable substrate useful in selectively attracting proteins, nucleating protein crystals and growing protein crystals of a size amenable to diffraction analysis. Further taught is the use of nanoscale assemblies to create charge patterns, where such charge patterns are useful in purifying, nucleating or crystallizing protein molecules. Charge extension moieties, including water, are taught. The invention provides rapid and efficient identification, purification and detection of proteins and protein-related complexes. | 10-30-2008 |
20080300148 | MICROFLUIDIC DEVICE FOR SIMULTANEOUSLY CONDUCTING MULTIPLE ANALYSES - Provided is a rotatable microfluidic device for conducting simultaneously two or more assays. The device includes a platform which can be rotated, a first unit which is disposed at one portion of the platform and detects a target material from a sample using surface on which a capture probe selectively binds to the target material is attached, and a second unit which is disposed at another portion of the platform and detects a target material included in the sample by a different reaction from the reaction conducted in the first unit. | 12-04-2008 |
20090005270 | Systems and Methods for Evaluating Enzyme Competency - The present invention provides systems and methods for determining enzymatic competency, which is important in determining whether a patient may suffer an adverse drug reaction, has a disease associated with defects in specific enzymatic function, and/or has an enzyme defect that is likely to cause pathophysiology. As contemplated herein, a parent molecular entity is administered to a patient in whom enzymatic competency is to be determined. A sample of the patient's bodily fluid is exposed to a sensor of the invention to distinguish, detect, and quantify a detectable entity in the bodily fluid. Sensor-acquired data regarding the detectable entity is used to determine enzymatic competency. Preferably, a sample of a patient's exhaled breath is collected and exposed to the sensor of the invention. Types of sensor systems of the invention include, but are not limited to, surface resonance arrays; microelectromechanical sensors (such as microcantilever-based technology); molecularly imprinted polymer sensors; amplifying fluorescent sensor technology; aptamer-based sensor technology; SAW sensors; infrared sensors; fuel cells; chemical reactors; and pH sensitive sensors. | 01-01-2009 |
20090023610 | Removable microfluidic cell - A microfluidic flow cell for removably interfacing with a removable-member for performing a reaction therebetween. The microfluidic flow cell device comprises at least one reaction portion defining with the removable-member a reaction chamber when in an interfaced position thereof. The microfluidic flow cell comprises at least one fluid-receiving portion for receiving a fluid therein and being in fluid communication with the reaction chamber. When the microfluidic flow cell and the removable-member are in the interfaced position, the cell is adapted to allow for the fluid in the fluid-receiving portion to flow to the reaction chamber. Devices, systems and methods comprising this microfluidic flow cell are also disclosed. | 01-22-2009 |
20090029877 | Automated System for Isolating, Amplifying, and Detecting a Target Nucleic Acid Sequence Present in a Fluid Sample - An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations, or modules, in which discrete aspects of the assay are performed on fluid samples contained in reaction receptacles. The analyzer includes stations for automatically preparing a specimen sample, incubating the sample at prescribed temperatures for prescribed periods, performing an analyte isolation procedure, and ascertaining the presence of a target analyte. An automated receptacle transporting system moves the reaction receptacles from one station to the next. The analyzer further includes devices for carrying a plurality of specimen tubes and disposable pipette tips in a machine-accessible manner, a device for agitating containers of target capture reagents comprising suspensions of solid support material and for presenting the containers for machine access thereto, and a device for holding containers of reagents in a temperature controlled environment and presenting the containers for machine access thereto. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte. The process is performed by automatically moving each of a plurality of reaction receptacles containing a solid support material and a fluid sample between stations for incubating the contents of the reaction receptacle and for separating the target analyte bound to the solid support from the fluid sample. An amplification reagent is added to the separated analyte after the analyte separation step and before a final incubation step. | 01-29-2009 |
20090036328 | BIOCHIP PACKAGE AND BIOCHIP PACKAGING SUBSTRATE - A biochip package allowing biochips optimized for high-volume production to be compatible with general-purpose devices and a biochip packaging substrate of the biochip package are provided. The biochip package can include a biochip having a probe array mounted thereon and a biochip packaging substrate on which the biochip is mounted and which has a through cavity exposing a rear surface of the biochip. | 02-05-2009 |
20090042743 | METHOD FOR DETERMINING CRYSTALLIZATION PARAMETERS AND APPARATUS FOR USE WITH THE SAME - The present disclosure provides a method to allow a user to pre-screen numerous crystallization conditions in the crystallization space to identify those conditions with the highest probability of yielding crystals and high quality diffracting crystals. In one embodiment, the dilute solution thermodynamic virial coefficient, termed B, is used to aid in the determination crystallization conditions that increase the probability of producing crystals for the crystallant of interest. The present disclosure also provide methods for predicting solution conditions that generate beneficial solubility and/or stability conditions for a polypeptide of interest using the B parameter. Devices for use in the described methods are also described. | 02-12-2009 |
20090042744 | MICRODEVICES FOR SCREENING BIOMOLECULES - Methods and devices for the parallel, in vitro screening of biomolecular activity using miniaturized microfabricated devices are provided. The biomolecules immobilized on the surface of the devices of the present invention include proteins, polypeptides, polynucleotides, polysaccharides, phospholipids, and related unnatural polymers of biological relevance. These devices are useful drug development, functional proteomics and clinical diagnostics and are preferably used for the parallel screening of families of related proteins. | 02-12-2009 |
20090042745 | BIOSENSOR CHIP - A biosensor chip is constituted by: a substrate; a metal film which is physically attached to the substrate; and a self assembled monolayer constituted by alkyl chains, which is physically attached to the metal film. Ligands and at least one functional group, selected from a group consisting of a hydroxyl group, a carboxyl group, an alkoxy group, a methyl group, and hydrogen atoms are physically attached to the self assembled monolayer. The ratio between the total number of alkyl chains physically attached to the metal film and the number of alkyl chains which are covalently bonded with the ligands is within a range of 0.40 to 0.99. | 02-12-2009 |
20090048125 | Biochip microsystem for bioinformatics recognition and analysis - A system with applications in pattern recognition, or classification, of DNA assay samples. Because DNA reference and sample material in wells of an assay may be caused to fluoresce depending upon dye added to the material, the resulting light may be imaged onto an embodiment comprising an array of photodetectors and an adaptive neural network, with applications to DNA analysis. Other embodiments are described and claimed. | 02-19-2009 |
20090054266 | Microfluidic transfer pin - A liquid dispenser for a microfluidic assay system is described. The dispenser includes at least one transfer pin for transferring a microfluidic sample of liquid to a target receptacle. A pin tip at one end of the transfer pin is structured to cooperate with an opening in the target receptacle. The tip uses capillary action to transfer the sample from the pin to the receptacle. | 02-26-2009 |
20090054267 | Active nems arrays for biochemical analyses - A biofunctionalized nanoelectromechanical device (BioNEMS) for sensing single-molecules in solution by measuring the variation in the mechanical displacement of the BioNEMS device during a binding event is provided. The biofunctionalized nanoelectromechanical device according to the invention generally comprises a nanomechanical mechanical resonator, a detector integral with the mechanical resonator for measuring the mechanical displacement of the resonator, and electronics connected to the detector for communicating the results to a user. A system of biofunctionalized nanoelectromechanical devices and a method for utilizing the biofunctionalized nanoelectromechanical device of the present invention are also provided. | 02-26-2009 |
20090062151 | COMPUTERIZED METHOD AND APPARATUS FOR ANALYZING AMINO ACIDS - A method for analyzing a plurality of amino acids in a fluid sample by a user is provided comprising the steps of introducing the sample into a buffer solution, passing the sample in the buffer solution through a separation column and setting a lithium ion concentration in the buffer to no more than 0.3 mols/L up to a time before β-aminoisobutyric acid (β-AiBA) is eluted. | 03-05-2009 |
20090062152 | THERMAL CYCLING APPARATUS AND METHOD - A thermal cycling device and a method of thermal cycling are provided. A thermal cycling device includes a fluid device system, a case, and a cycling head. The fluid delivery system develops a flow of controlled-temperature fluid. The case has a fluid-tight cavity for holding a microfluidic array. The array includes a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of through-holes running through the thickness between the surfaces. The cycling head holds the case and delivers the flow of fluid over the case. | 03-05-2009 |
20090069199 | METHOD AND DEVICE FOR OPTICAL DETECTION OF SUBSTANCES IN A LIQUID OR GASEOUS MEDIUM - The invention relates to a device for optical detection of substances in a liquid or gaseous medium, with a substrate with molecules for detecting the substances that are to be detected, wherein these molecules are immobilised at a surface of the substrate or in the substrate and wherein the substances that are to be detected can essentially be selectively bound to these molecules, wherein light waves can be coupled into the substrate and can be guided through this, and wherein the substrate is a foil element made of a transparent material in which a coupling structure for coupling the light waves is integrally formed and in which the coupled light waves can be guided. | 03-12-2009 |
20090075843 | Biological Sensor System - Disclosed is a system using each of the descrete emitters in a III-nitride micro-emitter array as a light source for measuring the properties of independent samples of biological materials deposed on a micro-array using some form of detecting device, e.g, a detector array or charge-coupled device. In embodiments the emitter array produces deep ultraviolet in investigating protein-protein interactions or to detect biological and chemical molecules with high specificity by monitoring changes in a protein's intrinsic fluorescence. | 03-19-2009 |
20090105095 | Device for Studying Individual Cells - A device for studying individual cells including a picowell array (such as an array of microwells, dimples, depressions, tubes or enclosures) and a fluid reservoir in fluid communication with the picowells through channels is disclosed. Preferably, the device has a moveable lid that in one rest location allows loading of cells in the picowell array. Preferably the channels of the device are capillary channels. | 04-23-2009 |
20090105096 | Filter-Equipped Microplate - There is provided a filter-equipped microplate including: an upper container | 04-23-2009 |
20090143250 | MICROFLUIDIC DEVICE USING MICROFLUIDIC CHIP AND MICROFLUIDIC DEVICE USING BIOMOLECULE MICROARRAY CHIP - Disclosed is a microfluidic device including a microfluidic structure formed in a platform in which various examinations, such as an immune serum examination, can be automatically performed using the biomolecule microarray chip. The biomolecule microarray chip-type microfluidic device using a biomolecule microarray chip comprises: a platform which is rotatable; a microfluidic structure disposed in the platform, comprising: a plurality of chambers; a plurality of channels connecting the chambers each other; and a plurality of valves controlling flow of fluids through the channels, wherein the microfluidic structure controls flow of a fluid sample using rotation of the platform and the valves; and a biomolecule microarray chip mounted in the platform such that biomolecule capture probes bound to the biomolecule microarray chip contact the fluid sample in the microfluidic structure. | 06-04-2009 |
20090186780 | Biochip - A biochip for qualitatively analyzing a biological sample using probes includes a probe cell array comprising a plurality of probe cells capable of coupling with biomolecules of a biological sample, an optical sensor for detecting optical signals from probe cells selectively coupling with the biomolecules of the biological sample and converting the optical signals to digital electrical signals, and a memory cell array for storing the digital electrical signals. | 07-23-2009 |
20090247427 | LABEL-FREE HIGH THROUGHPUT BIOMOLECULAR SCREENING SYSTEM AND METHOD - A screening system and method are described herein which provide a unique and practical solution for enabling label-free high throughput screening (HTS) to aid in the discovery of new drugs. In one embodiment, the screening system enables direct binding assays to be performed in which a biomolecular interaction of a chemical compound (drug candidate) with a biomolecule (therapeutic target) can be detected using assay volumes and concentrations that are compatible with the current practices of HTS in the pharmaceutical industry. The screening system also enables the detection of bio-chemical interactions that occur in the wells of a microplate which incorporates biosensors and surface chemistry to immobilize the therapeutic target at the surface of the biosensors. The screening system also includes fluid handling and plate handling devices to help perform automated HTS assays. | 10-01-2009 |
20090312200 | Nucleic Acid Analysis Chip Integrating a Waveguide and Optical Apparatus for the Inspection of Nucleic Acid Probes - A chip for nucleic acid analysis includes a body ( | 12-17-2009 |
20100022416 | ASSAY PLATES, METHODS AND SYSTEMS HAVING ONE OR MORE ETCHED FEATURES - The present invention includes composition, methods of making and methods of using a multi-nano-well plate having a first layer at least partially disposed on the substrate and one or more nano-wells that extends through the first layer that extends toward the substrate, wherein the one or more nano-wells having an opening in the first layer connected to bottom layer by one or more walls. | 01-28-2010 |
20100041571 | Multiplexed Lateral Flow Assay Arrays - A diagnostic test device comprising at least one membrane disposed in any of one, two, and three dimensions; at least one test sample fluid input port that receives and transfers at least one test sample fluid and onto the at least one membrane; at least one contiguous fluid flow manifold within the at least one membrane to multiplex or distribute the at least one test sample fluid; at least one contiguous fluid flow channel that operatively connects the at least one membrane to the at least one contiguous fluid flow manifold; at least one analyte assay test zone disposed within the at least one contiguous fluid flow channel; and at least one chemical capturing reagent disposed within the at least one analyte assay test zone, wherein multiple analyte assay test results are simultaneously obtained and in parallel in the diagnostic test device. | 02-18-2010 |
20100069265 | SYSTEM AND METHOD FOR PROCESSING LARGE NUMBER OF BIOLOGICAL MICROARRAYS - A system and method for processing biological sensors. The system includes a support component configured to support a fluidic component. The fluidic component includes at least a first container and a second container. The first container is capable of holding a first volume of a first fluid, and the second container is capable of holding a second volume of a second fluid. Additionally, the system includes a hybridization component configured to perform a hybridization process on a first sensor and a second sensor. Moreover, the system includes a transport component configured to move the first sensor, directly or indirectly, from the hybridization component into the first container and in contact with the first volume of the first fluid. | 03-18-2010 |
20100099582 | Biochip Package Structure - A biochip package structure is provided. The biochip package structure includes a substrate, a biochip, at least one wire, and a molding compound. The substrate has a circuit unit electrically connected, by wiring, to the biochip defined with a sensing region. The molding compound covers the wire but leaves the sensing region of the biochip exposed, allowing a cavity to be formed in the sensing region. The cavity delivers a biomedical sample. The biomedical sample reacts in the sensing region. Thus, the biochip package structure is applicable to various medical and biochemical assays. | 04-22-2010 |
20100099583 | OPTICAL DETECTING APPARATUS FOR A BIO-CHIP - An optical detecting apparatus for a bio-chip, the optical detecting apparatus including: a light source system for illuminating a bio-chip with an excitation light; a fluorescent light detecting system for detecting a fluorescent light emitted by the bio-chip; and a light path altering unit for directing the excitation light emitted by the light source system to a bio-chip and directing the fluorescent light emitted by the bio-chip to the fluorescent light detecting system, wherein a cross-sectional area of the excitation light irradiated by the light source system onto the bio-chip is greater than an area of the bio-chip, and the fluorescent light detecting system detects a fluorescent image of the entire bio-chip with a single illumination of excitation light. | 04-22-2010 |
20100105577 | PASSIVE MICROFLUIDIC ARRAY CARD AND READER - A microfluidic array card and reader system for analyzing a sample. The microfluidic array card includes a sample loading section for loading the sample onto the microfluidic array card, a multiplicity of array windows, and a transport section or sections for transporting the sample from the sample loading section to the array windows. The microfluidic array card reader includes a housing, a receiving section for receiving the microfluidic array card, a viewing section, and a light source that directs light to the array window of the microfluidic array card and to the viewing section. | 04-29-2010 |
20100105578 | METHOD AND DEVICE FOR MEASURING MULTIPLE PHYSIOLOGICAL PROPERTIES OF CELLS - A method of analyzing cells disposed in media within a vessel includes the steps of providing a vessel having an original volume of media about the cells, reducing the original volume of media about at least a portion of the cells to define a reduced volume of media, and analyzing a constituent related to the cells within the reduced volume of media. An apparatus for analyzing cells includes a stage adapted to receive a vessel holding cells and a volume of media, a plunger adapted to receive a barrier to create a reduced volume of media within the vessel including at least a portion of the cells, the barrier adapted for insertion into the vessel by relative movement of the stage and the plunger, and a sensor in sensing communication with the reduced volume of media, wherein the sensor is configured to analyze a constituent disposed within the reduced volume. | 04-29-2010 |
20100137166 | INDEPENDENTLY REMOVABLE NUCLEIC ACID SEQUENCING SYSTEM AND METHOD - A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected. | 06-03-2010 |
20100144558 | SYSTEMS AND METHODS FOR BIODOSIMETRY WITH BIOCHIP USING GENE EXPRESSION SIGNATURES - Exposure to ionizing radiation can produce a well-defined dose dependent signature in terms of changes in gene expression. In approaches and devices described herein, such a signature can be used to generate and use a self-contained radiation biodosimeter device, based on, for example, a blood finger stick. Various aspects of the invention are directed to biodosimetry with a fully integrated biochip using gene expression signatures. | 06-10-2010 |
20100160184 | CROSS TYPE MAGNETIC ARRAY SENSORS FOR BIOMOLECULES MAGNETIC BEAD DETECTION - There is provided a cross-type magnetic array sensor for biomolecules magnetic bead detection having a structure of various types, which can be used as a high-sensitivity and high-density magnetic biosensor. Since a vertical output voltage vertically crossing an applied current direction can be measured, it is possible to measure high-sensitivity magnetic-field variation in comparison with a known magnetic bead detection device that measures an output voltage parallel to the applied current direction. As a result, a magnetic bead magnetic-field detection device having a high signal-to-noise ratio can be achieved. Further, since the cross-type magnetic array sensors for biomolecules magnetic bead detection measure a vertical voltage, magnetic beads can be magnetized by an applied current induction magnetic field that is generated by an applied current without a magnetic field applied from the outside. | 06-24-2010 |
20100167959 | MICROELECTRONIC DEVICE WITH HEATING ARRAY - The invention relates to different designs of a microelectronic device comprising an array of heating elements (HE) with local driving units (CU | 07-01-2010 |
20100167960 | METHODS AND APPARATUS FOR SCREENING AND DETECTING MULTIPLE GENETIC MUTATIONS - An assay system and methods are described where patient samples containing genomic DNA are analyzed for the presence of known genetic polymorphisms using a universal reporter strategy. In a preferred embodiment, the amplified DNA is localized at test sites in an array of sites on a microchip followed by a series of hybridization reactions that screen for the presence of a single mutation from among a number of mutations, and allow the identification of specific mutations. In addition to universal reporters, the assay may use blockers and discriminators for screening and identification of known polymorphisms. | 07-01-2010 |
20100248995 | MICROREACTOR - A microreactor with at least one cavity, which comprises a bottom, a side wall and an opening disposed opposite the bottom, has a cross-section intersecting the side wall parallel to the bottom, the cross-section having a shape diverging from a round, square or rectangular shape. | 09-30-2010 |
20100267590 | Reaction Surface Array Diagnostic Apparatus - A reaction surface array diagnostic apparatus and method of making the same includes a substrate carrying a plurality of reaction surfaces, a plate and a gasket, each having a plurality of through bores, alignable with one of the reaction surfaces and forming a fluid tight well about each reaction surface when the gasket and the plate are sealingly affixed to the substrate to form a stack. Clamp members engage opposite side edges of a stack to compress the gasket. A plurality of side-by-side disposed clamped stacks of plates, gaskets and substrates are mounted in a tray in the standard footprint of a microtitre plate. Alternately, the plate and the gasket are combined into a single plate formed of a flexible material having an adhesive on one surface. | 10-21-2010 |
20100292105 | Methods and devices for concentration and fractionation of analytes for chemical analysis - A multi-well cassette configuration and an instrument capable of accepting the cassette and thereafter pre-concentrating and purifying analytes from biological samples held in the cassette wells, such as human serum, for subsequent analysis by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS). | 11-18-2010 |
20100323925 | Nanosensor array for electronic olfaction - A detector system is described including arrays having a plurality of nanoelectronic sensors comprising a channel including a nanostructured element disposed on a substrate, the nanostructured element functionalized by one or more materials disposed on or adjacent to the nanostructured element so as to operatively influence one or more sensor electrical properties. In certain embodiments, the nanostructured element comprise one or more nanotubes, and the functionalization material may include nanoparticles composed of one or more metals, metal oxides, salts, or other inorganic or organic materials or composites of these. | 12-23-2010 |
20100323926 | DIAGNOSIS DEVICE AND METHOD OF MANUFACTURING THE DIAGNOSIS DEVICE - Provided are a diagnosis device in which a bio-chemical reaction between a reference sample and a target sample occurs and a result of the bio-chemical reaction can be detected and a method of manufacturing the diagnosis device. The diagnosis device includes an image sensor where a plurality of photo-detectors are formed; a polymer layer which is made of a polymer material and formed on an upper portion of the image sensor; and a plurality of wells which are formed corresponding to the plurality of photo-detectors on the polymer layer, wherein an inner portion of each well is empty. | 12-23-2010 |
20100331219 | TEMPERATURE CONTROLLER FOR STRUCTURE - A temperature controller for controlling temperature of a first structure having a first flat face comprises a second structure having a second flat face for planar contact with the first flat face, a pressure-applying means for pressing the first structure, a second supporting means for supporting the second structure to be capable of changing inclination of the second flat face, a first supporting means for supporting the first structure by contact with a portion of the first structure other than the first flat face, and a temperature-controlling means, wherein the first structure is supported by pressing the first structure by the pressure-applying means, and the second structure is brought into contact with the temperature-controlling means with interposition of the second supporting means. | 12-30-2010 |
20110003718 | SELF-SENSING ARRAY OF MICROCANTILEVERS CHEMICAL DETECTION - The invention provides a chemical detection system for detecting at least one target chemical species, including a self-sensed cantilevered probe array having a plurality of self-sensed cantilevered probes, at least one chemical-sensitive coating material applied to at least one cantilevered probe in the cantilevered probe array, and an interface circuit that is coupled to the cantilevered probe array. At least one cantilevered probe in the cantilevered probe array exhibits a shifted cantilevered probe response when the cantilevered probe array is exposed to the target chemical species and the interface circuit actuates the cantilevered probe. A handheld chemical detection system and a method of operation are also disclosed. | 01-06-2011 |
20110009296 | NUCLEIC ACID SEQUENCING SYSTEM AND METHOD - A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected. | 01-13-2011 |
20110009297 | Consumable elements for use with fluid processing and detection systems - One embodiment describes an automated and flexible system to analyze probe arrays. It comprises a plurality of arrays mounted on pegs that are moved by an instrument handling robot to liquid reaction stations. | 01-13-2011 |
20110021383 | APPARATUSES FOR REAL-TIME, SINGLE MOLECULE SEQUENCE DETERMINATION - A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system. | 01-27-2011 |
20110028353 | BIOCHEMICAL REACTION CASSETTE - A biochemical reaction cassette having a structure for uniformizing a liquid flow in a reaction chamber with a simple additional arrangement is provided. A fluid resistive section is arranged in a channel that is constructed to include an injection port, a reaction chamber, and a discharge port. The fluid resistive section decreases the cross-sectional area of the channel to decrease the fluid resistance at ends in the width direction of the fluid resistive section and at positions remote from the injection port and the discharge port, where the flow rate is decreased. | 02-03-2011 |
20110046021 | TAG FREE BIO SENSING MICRO STRIP - In one embodiment, a system for determining information about an assay incorporates a filter assembly that includes an optical structure configured to be removably attachable to a laser resonating cavity of the system, the optical structure being configured to form a portion of the laser resonating cavity such that it reflects light propagating through a gain medium in the laser resonating cavity when attached thereto. The optical structure can include a first surface having an array of one or more sites, each site containing an assay, and a second surface configured such that the second surface is disposed within the laser resonating cavity forming a portion of the laser resonating cavity. The optical structure is configured to optically interact with the assay to change one or more characteristics of light in the laser resonating cavity based on a reaction that occurs in an assay on said first surface. | 02-24-2011 |
20110046022 | CRYSTALLIZATION DEVICE FOR HIGH-THROUGHPUT VISUAL INSPECTION AND X-RAY DIFFRACTION ANALYSIS - A crystallization chip has a two-dimensional substrate surface with modified wetting properties convenient for the evaluation of the crystallization experiment results by optical microscope and by X-ray diffraction. A crystallization chip mount comprises a base and a support structure that is connected to the base. The support structure provides a first channel that receives a portion of the crystallization chip. A crystallization chip holder comprises a body defining at least one slot to slidably receive the crystallization chip described herein. The at least one slot extends from an upper surface to a lower surface of the body. The crystallization chip holder further includes at least one support surface for each slot. For a given slot the at least one support surface is disposed on an edge of at least two surfaces of portions of the body that define the given slot. During use, the at least one support surface supports the crystallization chip prior to, during or after the formation of crystals. | 02-24-2011 |
20110059870 | Assay Plates, Reader Systems and Methods for Luminescence Test Measurements - Luminescence test measurements are conducted using an assay module having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence. | 03-10-2011 |
20110071055 | INTEGRATED CARTRIDGE - An integrated cartridge for sample processing and analysis is disclosed. The integrated cartridge contains a sample preparation chamber having a sample inlet and a sample outlet, and a sample purification chamber adapted to receive a replaceable sample purification unit containing a housing and an extraction filter inside the housing. The extraction filter specifically binds to a molecule of interest. The sample purification chamber has a sample inlet that is in fluid communication with the sample outlet of the sample preparation chamber. Also disclosed is a microarray-based sample analysis (MBSA) system. | 03-24-2011 |
20110111987 | CENTRIFUGAL MICROFLUIDIC SYSTEM FOR NUCLEIC ACID SAMPLE PREPARATION, AMPLIFICATION, AND DETECTION - A microfluidic system for processing a sample includes a microfluidic CD in the form a rotatable disc, the disc containing a plurality of separate lysis chambers therein. A magnetic lysis blade and lysis beads are disposed in each of the lysis chambers and a plurality of stationary magnets are disposed adjacent to and separate from the microfluidic CD. The stationary magnets are configured to magnetically interact with each of the magnetic lysis blades upon rotation of the microfluidic CD. Each lysis chamber may have its own separate sample inlet port or, alternatively, the lysis chambers may be connected to one another with a single inlet port coupled to one of the lysis chambers. Downstream processing may include nucleic acid amplification using thermoelectric heating as well as detection using a nucleic acid microarray. | 05-12-2011 |
20110118151 | MULTIPLE DISPLACEMENT AMPLIFICATION - The present invention provides methods kits and systems for performing multiple displacement amplification reactions. In one method a sample of nucleic acid is provided. The nucleic acid is contacted with a reaction mixture which includes a set of oligonucleotide primers, a one or more polymerase enzymes and a detergent. The reaction mixture is then subjected to conditions under which the nucleic acid sequence is amplified to produce an amplified product in a multiple displacement reaction. The method may also be carried out by contacting the nucleic acid with the reaction mixture in the form of an emulsion. A kit is also provided for carrying out either the methods described above. The kit includes one or more polymerases, a plurality of primers and a detergent. The kit may also include a hydrophobic polymer and may include instructions for performing a multiple displacement amplification reaction on a nucleic acid sample. | 05-19-2011 |
20110130310 | MICROBIOREACTOR AND MICROTITER PLATE COMPRISING A PLURALITY OF MICROBIOREACTORS - The present invention relates to a microbioreactor comprising a sample support ( | 06-02-2011 |
20110130311 | METHOD AND SYSTEM FOR DIAGNOSING VIRUS - Disclose herein is a method for diagnosing virus, comprising the steps of: (a) collecting a sample and lysing virus present in the sample; (b) treating the lysed sample with a specific protease to digest a protein in the sample into peptides; (c) measuring the masses of the peptides in the sample with a mass measurement device; and (d) comparing the masses of the peptides in the sample to the masses of peptides derived from known viral proteins digested with the same protease as used in step (b), thus identifying the protein from which the peptides of the sample were derived. Also disclosed is a system for diagnosing virus which can be used to carry out the above diagnostic method. | 06-02-2011 |
20110136699 | MANUFACTURING AND PROCESSING POLYMER ARRAYS - The invention provides methods to process multiple microarrays by providing a microarray plate and processing plates. In an embodiment of the invention, methods for assembling microarray plates by using wafers are described for high throughput microarray processing. | 06-09-2011 |
20110143968 | SAMPLE VESSELS - A sample vessel may include a segmented tubule and an interface receiving the tubule. The segmented tubule may include an opening for receiving a sample material and at least one compressible section, the at least one compressible section having a wall constructed at least partially from a material having sufficient flexibility to permit compression of opposed sections of the wall into contact with one another, and at least two segments of the tubule being fluidically isolated from one another by a bonding of a fluid-tight seal between opposed sections of the tubule wall, wherein said fluid-tight seal irreversibly opens upon application of fluid pressure greater than a threshold value to permit selective fluid communication between the opposed sections. The interface may facilitate delivery of a sample material to the tubule through the opening. | 06-16-2011 |
20110152127 | Light Transmitted Assay Beads - A micro bead having a digitally coded structure that is partially transmissive and opaque to light. The pattern of transmitted light is determined by to decode the bead. The coded bead may be structured a series of alternating light transmissive and opaque sections, with relative positions, widths and spacing resembling a 1D or 2D bar code image. To decode the image, the alternating transmissive and opaque sections of the body are scanned in analogous fashion to bar code scanning. The coded bead may be coated or immobilized with a capture or probe to effect a desired bioassay. The coded bead may include a paramagnetic material. A bioanalysis system conducts high throughput bioanalysis using the coded bead, including a reaction detection zone and a decoding zone. | 06-23-2011 |
20110166045 | WAFER SCALE PLASMONICS-ACTIVE METALLIC NANOSTRUCTURES AND METHODS OF FABRICATING SAME - Plasmonics-active nanostructure substrates—developed on a wafer scale in a reliable and reproducible manner such that these plasmonics-active nanostructures have nano-scale gaps (that include but are not limited to sub-10 nm gaps or sub-5 nm gaps) that provide the highest EM field enhancement between neighboring plasmonics-active metallic or metal-coated nanostructures. The plasmonics-active nanostructure substrates relate to environmental sensing based on SERS, SPR, LSPR, and plasmon enhanced fluorescence based sensing as well as for developing plasmonics enhanced devices such as solar cells, photodetectors, and light sources. Controllable development of sub-2 nm gaps between plasmonics-active nanostructures can also be achieved. Also, the size of the nano-scale gap regions can be tuned actively (e.g., by the application of voltage or current) to develop tunable sub-5 nm gaps between plasmonic nanostructures in a controllable manner. | 07-07-2011 |
20110172129 | Biochip Having Image Sensor with Back Side Illumination Photodiode - A biochip having an image sensor with a back side illumination photodiode structure includes: a biochip layer; and an image sensor layer attached to one surface of the biochip layer and configured to sense light with biochemical reaction information, which is emitted from the biochip layer, wherein the image sensor layer includes a plurality of light sensing parts which receive the light directed toward a back side of a wafer. | 07-14-2011 |
20110190170 | COMPOSITIONS FOR USE IN IDENTIFICATION OF ANTIBIOTIC-RESISTANT BACTERIA - The present invention relates generally to identification of antibiotic-resistant bacteria and provides methods, compositions and kits useful for this purpose when combined, for example, with molecular mass or base composition analysis. | 08-04-2011 |
20110195871 | SYSTEMS AND METHODS OF SCREENING BIOMARKERS IN BODILY FLUIDS - Systems and methods of screening biomarkers in bodily fluids. According to at least one embodiment of the system for analyzing stabilizing agents of the present disclosure, the system comprises a detection platform comprising a plurality of detection sites each capable of receiving a diagnostic marker, a stabilization agent, and a detection agent. The system, in at least one embodiment, further comprises a detection device capable of determining a binding characteristic between the detection agent and the diagnostic marker in the detection sites. Further, the system may also be able to compare the binding characteristic among a plurality of detection markers to order the level of effect of the stabilizing agents assayed on the binding characteristic between the detection agent and the diagnostic agent. | 08-11-2011 |
20110195872 | DEVICES AND SYSTEMS FOR BIOMARKER DETECTION - Devices and systems for biomarker detection . In at least one embodiment of a diagnostic testing device of the present disclosure, the device comprises a fluid collector to collect a body fluid, and a housing, to test and retain the body fluid. The housing, in at least one embodiment, comprises a collection chamber, having an open end, to receive the fluid collector and contain a stabilization reagent, at least one membrane test strip, to indicate the presence or absence of at least one diagnostic marker, and an immunoassay-based fingerprint acquisition pad in fluid communication with the collection chamber. | 08-11-2011 |
20110195873 | SYSTEMS AND METHODS FOR THE DETECTION OF BIOMARKERS - Systems and methods for the detection of biomarkers. In at least one embodiment of a microarray system of the present disclosure, the microarray system comprises a microarray product comprising at least 100 diagnostic markers/cm | 08-11-2011 |
20110201530 | Vacuum Assist For a Microplate - A vacuum assist apparatus can comprise a microplate. The microplate can comprise a first surface and an opposing second surface. A plurality of wells can be formed in the first surface of the microplate. Each of the plurality of wells can be sized to receive an assay therein. A support base can comprise a fluid passage. The microplate can be positioned adjacent and in contact with the support base. A pressure device, in fluid communication with the fluid passage, can exert a vacuum within the fluid passage to actively retain the microplate in the contact with the support base. | 08-18-2011 |
20110230375 | METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS - Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis. | 09-22-2011 |
20110237464 | Guided Mode Resonant Filter Biosensor Using a Linear Grating Surface Structure - Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters. | 09-29-2011 |
20110251110 | Methods for Sequencing Individual Nucleic Acids Under Tension - The invention provides apparatuses and methods of use thereof for sequencing nucleic acids subjected to a force, and thus considered under tension. The methods may employ but are not dependent upon incorporation of extrinsically detectably labeled nucleotides. | 10-13-2011 |
20110263463 | INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS - The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 μm or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region. In one embodiment, the characteristic of said chemical or biological sample is a concentration of a charged species and wherein each of said chemical field effect transistors is an ion-sensitive field effect transistor having a floating gate with a dielectric layer on a surface thereof, the dielectric layer contacting said sample fluid and being capable of accumulating charge in proportion to a concentration of the charged species in said sample fluid. In one embodiment such charged species is a hydrogen ion such that the sensors measure changes in pH of the sample fluid in or adjacent to the sample-retaining region thereof. Apparatus and chips of the invention may be adapted for large scale pH-based DNA sequencing and other bioscience and biomedical applications. | 10-27-2011 |
20110269648 | ELECTROCHEMICAL SENSOR SYSTEM - A electrochemical sensor system is provided. An example system utilizes electrical and steric properties of contaminants, such as pesticides, herbicides, and heavy metals to measure an ongoing concentration of multiple contaminants simultaneously in real time. An example system has a sensor array including sensors tuned to specific contaminants, each sensor having at least two conducting elements arranged in a capacitive relationship, for example, on a printed circuit board. A binding layer on the conducing elements of each sensor selectively binds a specific contaminant, which produces a signature change in a measureable electrical property, such as impedance. Enclosed sensors and chemical buffers preserve the chemical and physical environment of the contaminants for ongoing real-time measurement of dynamic concentrations. A delivery system enables samples containing contaminants to be automatically delivered to the array of sensors without adulterating the natural state of the samples. | 11-03-2011 |
20110269649 | ACTIVE NEMS ARRAYS FOR BIOCHEMICAL ANALYSES - A biofunctionalized nanoelectromechanical device (BioNEMS) for sensing single-molecules in solution by measuring the variation in the mechanical displacement of the BioNEMS device during a binding event is provided. The biofunctionalized nanoelectromechanical device according to the invention generally comprises a nanomechanical mechanical resonator, a detector integral with the mechanical resonator for measuring the mechanical displacement of the resonator, and electronics connected to the detector for communicating the results to a user. A system of biofunctionalized nanoelectromechanical devices and a method for utilizing the biofunctionalized nanoelectromechanical device of the present invention are also provided. | 11-03-2011 |
20110281774 | Gene Cluster Diagnosis Apparatus - A diagnosis apparatus is provided. A gene chip having a target gene cluster is used with the apparatus. The target gene cluster comprises a plurality of target genes related to a cancer. Thus, a fast, accurate, sensitive and cheap WEnCA-Chipball platform is provided for mass analysis and automatic operation while human error and time for diagnosis are both reduced. | 11-17-2011 |
20110281775 | DETECTION AND/OR QUANTIFICATION METHOD OF TARGET MOLECULES ON A SOLID SUPPORT - The present invention relates to a method and device for detecting and/or quantifying one or multiple target molecules present in a solution by quantifying online their binding on specific capture molecules immobilized at different locations (spots) of a surface of an optically transparent solid support without substantial detection of target molecules present in solution. The present invention allows multiple target assays to be performed in a simultaneous detection. More particularly, the invention comprises detecting in real-time the hybridization between capture DNA molecules present on a micro-array and target polynucleotides present in solution. The invention is also related to real-time PCR of multiple targets on a micro-array. | 11-17-2011 |
20110287980 | LASER MODIFICATION AND FUNCTIONALIZATION OF SUBSTRATES - Assay devices comprising substrates functionalized to comprise probe species on multiple separate regions are provided. Ten thousand to a hundred thousand separate regions can be provided in a substrate of one square centimeter. The separate regions can comprise separate probe species, or in another embodiment, multiple different probe species can be present on each single functionalized region. The probe species are selected to be specific for binding to target species of interest in a sample. Methods and systems for making these devices are also provided. The devices are useful, for example for assaying molecules in a human sample that are reactive to a large number of different allergens placed on the substrate. | 11-24-2011 |
20110287981 | METHODS AND SYSTEMS FOR SIMULTANEOUS REAL-TIME MONITORING OF OPTICAL SIGNALS FROM MULTIPLE SOURCES - Methods and systems for real-time monitoring of optical signals from arrays of signal sources, and particularly optical signal sources that have spectrally different signal components. Systems include signal source arrays in optical communication with optical trains that direct excitation radiation to and emitted signals from such arrays and image the signals onto detector arrays, from which such signals may be subjected to additional processing. | 11-24-2011 |
20110306522 | MICROFLUIDIC PROTEIN CRYSTALLOGRAPHY - The use of microfluidic structures enables high throughput screening of protein crystallization. In one embodiment, an integrated combinatoric mixing chip allows for precise metering of reagents to rapidly create a large number of potential crystallization conditions, with possible crystal formations observed on chip. In an alternative embodiment, the microfluidic structures may be utilized to explore phase space conditions of a particular protein crystallizing agent combination, thereby identifying promising conditions and allowing for subsequent focused attempts to obtain crystal growth. | 12-15-2011 |
20110312622 | MICROFLUIDIC DEVICE WITH LOW-VOLUME HYBRIDIZATION CHAMBERS FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET SEQUENCES - A microfluidic device for detecting target nucleic acid sequences in a sample, the microfluidic device having an array of hybridization chambers, each containing electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences, and electrode pairs for receiving an electrical pulse, the ECL probes being configured to emit a photon of light when hybridized with one of the nucleic acid targets and activated by one of the electrodes, wherein, the hybridization chambers each have a volume less than 900000 cubic microns. | 12-22-2011 |
20110312623 | LOC DEVICE FOR PATHOGEN DETECTION WITH DIALYSIS, THERMAL LYSIS, NUCLEIC ACID AMPLIFICATION AND PREHYBRIDIZATION FILTERING - A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a first dialysis section for separating pathogens from larger constituents in the sample, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section having a lysis chamber and a heater for lysing the pathogens while the sample is in the lysis chamber, a nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material, and, a second dialysis section downstream of the nucleic acid amplification section for prehybridization filtration of amplicon produced by the nucleic acid amplification section, the second dialysis section being configured to remove cell debris from the amplicon, wherein, the first dialysis section, the lysis section, the nucleic acid amplification section and the second dialysis section are all supported on the supporting substrate. | 12-22-2011 |
20110312624 | MICROFLUIDIC DEVICE WITH FLOW-CHANNEL STRUCTURE HAVING ACTIVE VALVE FOR CAPILLARY-DRIVEN FLUIDIC PROPULSION WITHOUT TRAPPED AIR BUBBLES - A microfluidic device for processing a fluid sample, the microfluidic device having a first channel configured to fill with the sample by capillary action, a second channel configured to fill with the sample by capillary action, a plurality of fluid connections between the first channel and the second channel, one of the fluid connections having an active valve to arrest the sample flow into the second channel and the remaining fluid connections each being configured to pin a meniscus of the sample that arrests capillary flow between the first channel and the second channel, the fluid connection with the active valve being upstream of the remaining fluid connections, wherein during use, the sample flow into the second channel is initiated by activation of the active valve such that the sample flow in the second channel progressively removes the meniscus at each of the meniscus anchors. | 12-22-2011 |
20110312625 | LOC DEVICE FOR GENETIC ANALYSIS WITH DIALYSIS, CHEMICAL LYSIS AND TANDEM NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating cells larger than a predetermined threshold in the sample from smaller constituents, whereby the cells larger than a predetermined threshold include target cells containing genetic material for analysis, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the target cells to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the target cells in the lysis section, a first nucleic acid amplification section downstream of the lysis section for amplifying first nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the first nucleic acid amplification section for amplifying second nucleic acid sequences in the amplicon from the first nucleic acid amplification section, wherein, the dialysis section, the lysis section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312626 | TEST MODULE INCORPORATING SPECTROMETER - A test module having an outer casing having a receptacle for receiving a biological sample, an excitation source mounted in the outer casing for sequentially illuminating the biological sample with different wavelengths of light, and, a photosensor positioned to detect the different wavelengths of light transmitted through the biological sample, wherein during use, the photosensor output signal is used to generate a spectrogram for analysis of a characteristic of the biological sample. | 12-22-2011 |
20110312627 | TEST MODULE WITH MICROFLUIDIC DEVICE HAVING LOC AND DIALYSIS DEVICE FOR SEPARATING PATHOGENS FROM OTHER CONSTITUENTS IN A BIOLOGICAL SAMPLE - A test module for concentrating pathogens in a biological sample, the test module having an outer casing with a receptacle for receiving the sample, a dialysis device in fluid communication with the receptacle and configured to separate the pathogens from other constituents in the sample, and, a lab-on-a-chip (LOC) device being in fluid communication with the dialysis device and configured to analyze the pathogens. | 12-22-2011 |
20110312628 | MICROFLUIDIC DEVICE WITH MST LAYER AND OVERLYING CAP - A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer on the supporting substrate, a cap overlying the MST layer, the cap having a plurality of fluidic connections between the cap and the MST layer for fluid flow from the MST layer to the cap and fluid flow from the cap to the MST layer. | 12-22-2011 |
20110312629 | MICROFLUIDIC DEVICE WITH DIALYSIS SECTION HAVING STOMATA TAPERING COUNTER TO FLOW DIRECTION - A microfluidic device for removing cell debris from a biological sample, the microfluidic device having a dialysis section with a large constituent channel and a small constituent channel, and a series of stoma for fluid communication between the large constituent channel and the small constituent channel, the large constituent channel having an upstream end for receiving the biological sample, the biological sample being a liquid carrying a mixture of cell debris and target molecules, the small constituent channel having a downstream end for connection to a hybridization section with an array of probes for reaction with the target molecules to form probe-target complexes, wherein, each of the stoma is tapered in a counter-flow direction such that each have a small opening to the large constituent channel and a large opening to the small constituent channel, the small openings being sized to allow the target molecules to flow into the small constituent channel but retain the cell debris larger than a threshold size in the large constituent channel. | 12-22-2011 |
20110312630 | LOC DEVICE FOR PATHOGEN DETECTION, GENETIC ANALYSIS AND PROTEOMIC ANALYSIS WITH DIALYSIS, CHEMICAL LYSIS, INCUBATION AND TANDEM NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for pathogen detection, genetic analysis and proteomic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a leukocyte dialysis section for dividing the sample into a leukocyte stream, and a pathogen and erythrocyte stream containing cells and sample constituents smaller than a first size threshold, a pathogen dialysis section for dividing the pathogen and erythrocyte stream into an erythrocyte stream and a pathogen stream containing cells and sample constituents smaller than a second size threshold, a leukocyte lysis section downstream of the leukocyte dialysis section for lysing the leukocytes with a lysis reagent to release genetic material and proteins therein, a leukocyte incubation section downstream of the leukocyte lysis section for enzymatic reaction of the genetic material with enzymes, a pathogen lysis section downstream of the pathogen dialysis section for lysing the pathogens with a lysis reagent to release genetic material and proteins therein, a pathogen incubation section downstream of the pathogen lysis section for enzymatic reaction of the genetic material with enzymes, an erythrocyte lysis section downstream of the pathogen dialysis section for lysing the erythrocytes with a lysis reagent to release proteins therein, a first leukocyte nucleic acid amplification section downstream of the leukocyte incubation section for amplifying nucleic acid sequences, a second leukocyte nucleic acid amplification section downstream of the first leukocyte nucleic acid amplification section for amplifying nucleic acid sequences, a first pathogen nucleic acid amplification section downstream of the pathogen incubation section for amplifying nucleic acid sequences, and, a second pathogen nucleic acid amplification section downstream of the first pathogen nucleic acid amplification for amplifying nucleic acid sequences, wherein, the leukocyte dialysis section, the pathogen dialysis section, the leukocyte lysis section, the pathogen lysis section, the leukocyte incubation section, the pathogen incubation section, the first and second leukocyte nucleic acid amplification sections and the first and second pathogen nucleic acid amplification sections are all supported on the supporting substrate. | 12-22-2011 |
20110312631 | GENETIC ANALYSIS LOC WITH NON-SPECIFIC NUCLEIC ACID AMPLIFICATION SECTION AND SUBSEQUENT SPECIFIC AMPLIFICATION OF PARTICULAR SEQUENCES IN A SEPARATE SECTION - A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing dNTP's, primers, polymerase and buffer solution for addition to the sample, a first nucleic acid amplification section for thermal control of the sample to amplify the target nucleic acid sequences, and, a second nucleic acid amplification section for thermal control of amplicon from the first nucleic acid amplification section to further amplify the target nucleic acid sequences. | 12-22-2011 |
20110312632 | MICROFLUIDIC DEVICE WITH PCR SECTION AND DIFFUSION MIXER - A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, a reagent reservoir containing a reagent, and, a diffusion mixing section for mixing the nucleic acid sequences with the reagent, the diffusion mixing section having a microchannel defining a tortuous flow-path having a length sufficient for diffusive mixing of the reagent and the sample, wherein during use, the sample flows from the sample inlet to the PCR section via the diffusion mixing section. | 12-22-2011 |
20110312633 | MICROFLUIDIC DEVICE WITH DIALYSIS SECTION - A microfluidic device for dialysis of biological material, the microfluidic device having a sample inlet for receiving a sample of biological material that has constituents of different sizes, a dialysis section for separating smaller constituents from larger constituents, the smaller constituents being smaller than a predetermined size threshold, and the larger constituents being larger than the predetermined size threshold, and, a microsystems technology (MST) layer for separately processing the smaller constituents and/or the larger constituents. | 12-22-2011 |
20110312634 | MICROFLUIDIC DEVICE WITH LAMINAR STRUCTURE - A microfluidic device having a supporting substrate, a microsystems technology (MST) layer overlying the supporting substrate, the MST layer defining MST channels for fluid flow within the MST layer, a cap overlying the MST layer, the cap defining cap channels for fluid flow within the cap and reservoirs for holding a quantity of fluid, wherein, the MST channels and the cap channels are in fluid communication. | 12-22-2011 |
20110312635 | MICROFLUIDIC DEVICE WITH FLOW-CHANNEL STRUCTURE FOR CAPILLARY-DRIVEN FLUIDIC PROPULSION WITHOUT TRAPPED AIR BUBBLES - A microfluidic device for processing a fluid sample, the microfluidic device having a first channel configured to fill with the sample by capillary action, a second channel configured to fill with the sample by capillary action, a plurality of fluid connections between the first channel and the second channel, each of the fluid connections being configured to pin a meniscus of the sample that arrests capillary flow between the first channel and the second channel, a bypass channel between the first channel and the second channel, the bypass channel joining the second channel upstream of the plurality of fluid connections and is configured for uninterrupted capillary driven flow from the first channel to the second channel, wherein during use, flow from the bypass channel reaches the meniscus pinned at each of the fluid connections after the meniscus has formed such that the flow sequentially removes each of the menisci and sample flow from the first channel to the second channel is via the plurality of fluid connections as well as the bypass channel. | 12-22-2011 |
20110312636 | LOC DEVICE WITH DIALYSIS SECTION FOR SEPARATING LEUKOCYTES FROM BLOOD - A lab-on-a-chip (LOC) device to for analyzing leukocytes in a blood sample, the LOC device having a supporting substrate, an inlet for receiving a sample of blood including leukocytes and erythrocytes, a dialysis section for separating at least some of the erythrocytes from the leukocytes, and, a microsystems technology (MST) layer for separately analyzing the leukocytes, wherein, the inlet, the dialysis section and the MST layer are all supported on the supporting substrate. | 12-22-2011 |
20110312637 | LOC DEVICE WITH DIALYSIS SECTION FOR SEPARATING PATHOGENS FROM A BIOLOGICAL SAMPLE - A lab-on-a-chip (LOC) device to separate pathogens from a biological sample for analysis, the LOC device having a supporting substrate, an inlet for receiving the sample including pathogens and other constituents larger than the pathogens, a dialysis section for separating the pathogens into a portion of the sample, and, a microsystems technology (MST) layer for separately analyzing the pathogens, wherein, the inlet, the dialysis section and the MST layer are all supported on the supporting substrate. | 12-22-2011 |
20110312638 | LOC DEVICE FOR PATHOGEN DETECTION AND GENETIC ANALYSIS WITH DIALYSIS AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for pathogen detection and genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a plurality of reagent reservoirs, a dialysis section for separating pathogens and cells larger than a predetermined threshold in the sample from smaller constituents, whereby the pathogens and cells larger than the predetermined threshold contain genetic material for analysis, a nucleic acid amplification section downstream of the dialysis section for amplifying nucleic acid sequences from the genetic material, wherein, the dialysis section and the nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312639 | LOC DEVICE WITH DIALYSIS SECTION FOR SEPARATING LEUKOCYTES AND PATHOGENS FROM BLOOD - A lab-on-a-chip (LOC) device to separate pathogens and leukocytes from a blood sample for analysis, the LOC device having a sample inlet for receiving the blood including pathogens, leukocytes and other constituents, a dialysis section for separating the pathogens into a first portion of the sample and separating the leukocytes into a second portion of the sample such that the remainder of the sample has constituents smaller than the leukocytes and larger than the pathogens, and, a microsystems technology (MST) layer for analyzing the pathogens and the leukocytes. | 12-22-2011 |
20110312640 | MICROFLUIDIC DEVICE WITH PHOTOSENSOR - A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer for processing a fluid sample, and, a photosensor adjacent the MST layer for detecting photons emitted from the MST layer. | 12-22-2011 |
20110312641 | MICROFLUIDIC DEVICE WITH SAMPLE INLET AND PROBE HYBRIDIZATION SECTION - A microfluidic device for detecting hybridization of probes with target nucleic acid sequences, the microfluidic device having a sample inlet for receiving a fluid sample with target nucleic acid sequences, an array of probes for hybridization with the target nucleic acid sequences, and, a flow-path from the sample inlet to the array, wherein, the flow-path is configured to draw the fluid sample from the sample inlet to the probes by capillary action. | 12-22-2011 |
20110312642 | MICROFLUIDIC DEVICE FOR DETECTION OF NUCLEIC ACID TARGETS WITH ELECTROCHEMILUMINESCENT PROBES - A microfluidic device for detecting a target nucleic acid sequence in a sample, the microfluidic device having a sample inlet for receiving the sample, probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, and, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, wherein, the sample inlet draws the sample along a fluid flow-path leading to the probes by capillary action. | 12-22-2011 |
20110312643 | MICROFLUIDIC DEVICE FOR DETECTION OF HYBRIDIZATION OF NUCLEIC ACID TARGETS - A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer with a hybridization section that has an array of probes for hybridization with target nucleic acid sequences to form probe-target hybrids, and, a photosensor for detecting the probe-target hybrids. | 12-22-2011 |
20110312644 | MICROFLUIDIC DEVICE FOR SIMULTANEOUS DETECTION OF MULTIPLE CONDITIONS IN A PATIENT - A microfluidic device for simultaneous detection of multiple conditions in a patient, the microfluidic device having an inlet for receiving a sample of biological material drawn from the patient, a microsystems technologies (MST) layer with a detection section having an array of probes for reaction with target molecules in the sample to form probe-target complexes, the target molecules being indicative of medical conditions in the patient, and, a photosensor for detecting the probe-target complexes, wherein, the array of probes has more than 1000 probes. | 12-22-2011 |
20110312645 | MICROFLUIDIC DEVICE WITH TEMPERATURE FEEDBACK CONTROLLED HYBRIDIZATION CHAMBERS FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGETS - A microfluidic device for detecting target nucleic acid sequences in a sample, the microfluidic device having a supporting substrate, a microsystems technology (MST) layer overlying the supporting substrate for processing the sample, the MST layer having an array of hybridization chambers, each containing electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences, and electrode pairs for receiving an electrical pulse, the ECL probes being configured to emit a photon of light when hybridized with one of the nucleic acid targets and activated by one of the electrodes, an array of temperature sensors positioned such that at least one of the temperature sensors corresponds to each of the hybridization chambers respectively, and, heaters for heating each of the hybridization chambers, such that, output from the temperature sensors is used for feedback control of the heaters. | 12-22-2011 |
20110312646 | LOC DEVICE FOR SEPARATING CONSTITUENTS OF INTERMEDIATE SIZE FROM LARGER AND SMALLER CONSTITUENTS IN A BIOLOGICAL SAMPLE - A lab-on-a-chip (LOC) device to separate constituents of intermediate size from larger and smaller constituents in a biological sample, the LOC device having an inlet for receiving the biological sample including large constituents sized above an upper threshold, small constituents sized smaller than a lower threshold and intermediate constituents sized between the upper and the lower thresholds, a first channel in fluid communication with the inlet, first apertures sized to correspond to the upper threshold, a second channel in fluid communication with the first channel via the first apertures, second apertures sized to correspond to the lower threshold, a third channel in fluid communication with the second channel via the second apertures, and, a microsystems technology (MST) layer for analyzing the large, intermediate and or small constituents separately, wherein during use, the intermediate and the small constituents flow into the second channel via the first apertures while the large constituents are retained in the first channel, and the small constituents flow into the third channel while the intermediate constituents are retained in the second channel | 12-22-2011 |
20110312647 | MICROFLUIDIC DEVICE WITH TEMPERATURE FEEDBACK CONTROLLED HYBRIDIZATION CHAMBERS - A microfluidic device having a supporting substrate, a microsystems technology (MST) layer overlying the supporting substrate for processing a fluid containing target nucleic acid sequences, the MST layer having an array of hybridization chambers, each containing probes for hybridization with the target nucleic acid sequences, a temperature sensor for sensing the temperature at the array of hybridization chambers, and, a hybridization heater for heating the array of hybridization chambers, wherein during use, output from the temperature sensor is used for feedback control of the hybridization heater. | 12-22-2011 |
20110312648 | MICROFLUIDIC DEVICE FOR GENETIC AND MITOCHONDRIAL ANALYSIS OF A BIOLOGICAL SAMPLE - A microfluidic device for analysis of genetic DNA and mitochondrial DNA in a sample, the microfluidic device having an inlet for receiving a sample of biological material having cells with an outer cellular membrane containing mitochondria and a nucleus, the mitochondria has a mitochondrial membrane containing mitochondrial DNA and the nuclei have a nuclear membrane containing nuclear DNA, a first lysis section for lysing the outer cellular membrane and the mitochondrial membrane while the nuclear membrane remains intact, a second lysis section for lysing the nuclear membrane, mitochondrial probes downstream of the first lysis section for hybridization with target nucleic acid sequences in the mitochondrial DNA to form probe-target hybrids, and, nuclear probes downstream of the second lysis second for hybridization with target nucleic acid sequences in the nuclear DNA to form probe-target hybrids. | 12-22-2011 |
20110312649 | MICROFLUIDIC DEVICE WITH OPTICALLY TRANSPARENT HYBRIDIZATION CHAMBERS - A microfluidic device having a supporting substrate, a microsystems technology (MST) layer overlying the supporting substrate for processing a fluid containing target nucleic acid sequences, the MST layer having an array of hybridization chambers, each containing probes for hybridization with the target nucleic acid sequences, the probes being configured to emit a fluorescence signal in response to an excitation light upon hybridization, wherein, the hybridization chamber has a wall section that is optically transparent to the fluorescence signal. | 12-22-2011 |
20110312650 | MICROFLUIDIC DEVICE WITH OPTICALLY TRANSPARENT HYBRIDIZATION CHAMBERS FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGETS - A microfluidic device for detecting target nucleic acid sequences in a sample, the microfluidic device having an array of hybridization chambers, each containing electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences, and electrode pairs for receiving an electrical pulse, the ECL probes being configured to emit a photon of light when hybridized with one of the nucleic acid targets and activated by one of the electrodes, wherein, the hybridization chambers each have a wall section that is optically transparent to the light emitted by the ECL probes. | 12-22-2011 |
20110312651 | MICROFLUIDIC DEVICE WITH LOW MASS PROBE SPOTS - A microfluidic device having a supporting substrate, a microsystems technology (MST) layer overlying the supporting substrate for processing a fluid containing target nucleic acid sequences, the MST layer having a plurality of probe spots, each of the probe spots containing probes for hybridization with the target nucleic acid sequences, wherein, the mass of the probes in each of the probe spots is less than 270 picograms. | 12-22-2011 |
20110312652 | MICROFLUIDIC DEVICE WITH LOW-VOLUME ELECTROCHEMILUMINESCENCE-BASED PROBE SPOTS - A microfluidic device for detecting target nucleic acid sequences in a sample, the microfluidic device having an array of hybridization chambers, each of the hybridization chambers containing electrode pairs for receiving an electrical pulse and electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences, the ECL probes being configured to emit a photon of light when hybridized with one of the nucleic acid targets and excited by current between the electrodes, wherein, the mass of the probes in each of the hybridization chambers is less than 270 picograms. | 12-22-2011 |
20110312653 | MICROFLUIDIC DEVICE WITH LOW-VOLUME HYBRIDIZATION CHAMBERS - A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer with a hybridization section that has an array of hybridization chambers, each containing a different probe type for hybridization with target nucleic acid sequences, wherein, each of the hybridization chambers has a volume less than 900,000 cubic microns. | 12-22-2011 |
20110312654 | APPARATUS FOR LOADING OLIGONUCLEOTIDE SPOTTING DEVICES AND SPOTTING OLIGONUCLEOTIDE PROBES - An apparatus for loading oligonucleotide spotting devices and spotting oligonucleotide probes, the apparatus having a plurality of oligonucleotide vials, each with a droplet dispenser, a mounting surface for detachably mounting an oligonucleotide spotting device, a chuck for detachably mounting the oligonucleotide spotting device adjacent the mounting surface, and, a control processor for operative control of the oligonucleotide vials, the oligonucleotide spotting device when mounted in the chuck and movement of the mounting surface relative to the oligonucleotide vials, and the oligonucleotide spotting device, wherein, the control processor is configured to activate the droplet dispensers, and move the oligonucleotide spotting device into registration with the oligonucleotide vials. | 12-22-2011 |
20110312655 | MICROFLUIDIC DEVICE FOR PCR, PROBE HYBRIDIZATION AND ELECTROCHEMILUMINESCENT DETECTION OF PROBE-TARGET HYBRIDS - A microfluidic device for amplifying and detecting target nucleic acid sequences in a sample, the microfluidic device having a polymerase chain reaction (PCR) section for amplifying the target nucleic acid sequences, and, a hybridization section that has an array of electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, and a plurality of electrodes for receiving an electrical pulse such that the probe-target hybrids emit a photon of light. | 12-22-2011 |
20110312656 | MICROFLUIDIC DEVICE FOR PCR AND PROBE HYBRIDIZATION - A microfluidic device having a sample inlet for receiving a sample of biological material containing target nucleic acid sequences, and, a microsystems technologies (MST) layer with a polymerase chain reaction (PCR) section for amplifying the target nucleic acid sequences, and an array of probes for hybridization with the target nucleic acid sequences. | 12-22-2011 |
20110312657 | MICROFLUIDIC TEST MODULE FOR INTERFACING WITH A MOBILE TELEPHONE - A microfluidic test module for analyzing a sample fluid and communicating test results to a mobile telephone, the microfluidic test module having an outer casing with a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the mobile telephone, and, a controller for operative control of the communication interface. | 12-22-2011 |
20110312658 | LOC DEVICE WITH DIALYSIS SECTION FOR CONCENTRATING NUCLEATED CELLS IN A BIOLOGICAL SAMPLE - A lab-on-a-chip (LOC) device for concentrating nucleated cells in a biological sample, the LOC device having an inlet for receiving a sample of biological material including nucleated cells and other constituents, the nucleated cells all being larger than a predetermined threshold, a dialysis section for concentrating the nucleated cells into a portion of the sample such that the remainder of the sample contains the other constituents smaller than the predetermined threshold, and, a microsystems technology (MST) layer for processing the nucleated cells. | 12-22-2011 |
20110312659 | MICROFLUIDIC DEVICE WITH HYBRIDIZATION CHAMBERS AND CORRESPONDING DIFFUSION BARRIERS - A microfluidic device having a sample flow-path for a biological sample containing target nucleic acid sequences, an array of hybridization chambers each containing probes for hybridization with the target nucleic acid sequences, each of the hybridization chambers having a chamber inlet for fluid communication between the sample flow-path and the probes, wherein, the chamber inlet is configured as a diffusion barrier to prevent diffusion of hybridized probes between the hybridization chambers to an extent that causes erroneous hybridization detection results. | 12-22-2011 |
20110312660 | MICROFLUIDIC TEST MODULE FOR INTERFACING WITH A LAPTOP COMPUTER - A microfluidic test module for analyzing a sample fluid and communicating test results to a laptop computer, the microfluidic test module having an outer casing with a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the laptop computer, and, a controller for operative control of the communication interface. | 12-22-2011 |
20110312661 | MICROFLUIDIC DEVICE WITH ARRAY OF CHAMBERS AND CORRESPONDING DIFFUSION BARRIERS FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGETS - A microfluidic device for detecting target molecules in a fluid, the microfluidic device having an array of chambers, each containing electrochemiluminescent (ECL) probes for reaction with the target molecules to form a probe-target complex, and electrodes positioned in each of the chambers for receiving an electrical pulse, the probe-target complexes being configured to emit a photon of light when excited by current between the electrodes, a flow-path for the fluid containing the targets, wherein, each of the chambers have a chamber inlet for fluid communication between the sample flow-path and the probes within the chamber, the chamber inlet being configured as a diffusion barrier to prevent diffusion of the probe-target complexes between the chambers to an extent that causes erroneous detection results. | 12-22-2011 |
20110312662 | LOC DEVICE WITH DIALYSIS SECTION FOR REMOVING CELL DEBRIS FROM A BIOLOGICAL SAMPLE - A lab-on-a-chip (LOC) device for removing cell debris from a biological sample, the LOC device having a dialysis section with a large constituent channel, a small constituent channel and a plurality of apertures for fluid communication between the large constituent channel and the small constituent channel, the large constituent channel having an upstream end for receiving the biological sample, the biological sample being a liquid carrying a mixture of cell debris and target molecules, the small constituent channel having a downstream end for connection to a hybridization section with an array of probes for reaction with the target molecules to form probe-target complexes, wherein, the apertures are sized to allow the target molecules to flow into the small constituent channel but retain the cell debris larger than a threshold size in the large constituent channel. | 12-22-2011 |
20110312663 | MICROFLUIDIC DEVICE WITH TIME DELAYED DETECTION OF FLUORESCENCE FROM HYBRIDIZED PROBES - A microfluidic device having a supporting substrate, a probe having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, CMOS circuitry on the substrate, the CMOS circuitry having a photosensor for generating an output signal in response to the fluorescence signal, wherein, the CMOS circuitry is configured to trigger a time delay when the excitation light is deactivated before activating the photosensor. | 12-22-2011 |
20110312664 | MICROFLUIDIC TEST MODULE FOR INTERFACING WITH A DEDICATED READER - A microfluidic test module for analyzing a sample fluid and communicating test results to a dedicated reader purpose built for operating with the microfluidic test module, the microfluidic test module having an outer casing with a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the dedicated reader, and, a controller for operative control of the communication interface. | 12-22-2011 |
20110312665 | LOC WITH DIALYSIS SECTION FOR REMOVING INSOLUBLE SAMPLE CONSTITUENTS FROM A NUCLEIC ACID MIXTURE - A lab-on-a-chip (LOC) device for removing cell debris from a biological sample containing target nucleic acid sequences, the LOC device having a dialysis section with a large constituent channel, a small constituent channel and a plurality of apertures for fluid communication between the large constituent channel and the small constituent channel, the large constituent channel having an upstream end for receiving the biological sample, the biological sample being a liquid carrying a mixture of cell debris and target nucleic acid sequences, and the small constituent channel having a downstream end for connection to a hybridization section with an array of probes for hybridization with the target nucleic acid sequences to form probe-target complexes, wherein, the apertures are sized to allow the target nucleic acid sequences to flow into the small constituent channel but retain the cell debris larger than a threshold size in the large constituent channel. | 12-22-2011 |
20110312666 | MICROFLUIDIC DEVICE WITH TRIGGERED PHOTODETECTION OF FLUORESCING PROBE-TARGET HYBRID - A microfluidic device having a probe for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid having a reporting fluorophore for emitting a fluorescence signal in response to an excitation light, a detection photodiode for exposure to the excitation light and the fluorescence signal, and, a trigger photodiode for exposure to the excitation light, the trigger photodiode being configured to activate the detection photodiode after the excitation light has been extinguished. | 12-22-2011 |
20110312667 | MICROFLUIDIC TEST MODULE FOR INTERFACING WITH A DESKTOP COMPUTER - A microfluidic test module for analyzing a sample fluid and communicating test results to a desktop computer, the microfluidic test module having an outer casing with a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the desktop computer, and, a controller for operative control of the communication interface. | 12-22-2011 |
20110312668 | LOC WITH DIALYSIS SECTION FOR RETAINING INSOLUBLE SAMPLE CONSTITUENTS AFTER AMPLIFICATION AND PASSING SOLUBLE CONSTITUENTS TO A DETECTION SECTION - A lab-on-a-chip (LOC) device for removing cell debris from a biological sample containing target nucleic acid sequences, the LOC device having an amplification section for amplifying the nucleic acid sequences, a dialysis section with a large constituent channel, a small constituent channel and a plurality of apertures for fluid communication between the large constituent channel and the small constituent channel, the large constituent channel configured to receive the biological sample after nucleic acid amplification in the amplification section, the biological sample being a liquid mixture of the cell debris and the target nucleic acid sequences and the apertures are sized to allow the target nucleic acid sequences to flow into the small constituent channel while the cell debris larger than a threshold size is retained in the large constituent channel, and, an array of probes in fluid communication with the small constituent channel for hybridization with the target nucleic acid sequences to form probe-target hybrids. | 12-22-2011 |
20110312669 | MICROFLUIDIC DEVICE WITH ELECTROCHEMILUMINESCENT PROBES AND PHOTOSENSOR WITH LARGE ANGLE OF COLLECTION FOR PROBE EMITTTED LIGHT - A microfluidic device for detecting target molecules in a fluid, the microfluidic device having a hybridization chamber containing electrodes and probes for hybridization with target molecules to form probe-target complexes and generate an electrochemiluminescence (ECL) signal in response to an electrical current between the electrodes, and, a photodiode with an active area and an optical axis extending normal to the active area and through the hybridization chamber, wherein, the hybridization chamber has a floor surface positioned parallel to the active area of the photodiode, the floor surface having a centroid and the active area being encompassed within a cone having the centroid at its vertex, and a vertex angle less than 174°. | 12-22-2011 |
20110312670 | MICROFLUIDIC TEST MODULE FOR INTERFACING WITH AN EBOOK READER - A microfluidic test module for analyzing a sample fluid and communicating test results to an ebook reader, the microfluidic test module having an outer casing with a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the ebook reader, and, a controller for operative control of the communication interface. | 12-22-2011 |
20110312671 | SINGLE USE MICROFLUIDIC DEVICE WITH PHOTOSENSOR FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGETS - A microfluidic device for detecting target molecules in a fluid, the microfluidic device having an array of electrochemiluminescent (ECL) probes for reaction with the target molecules to form a probe-target complexes, electrodes for receiving an electrical pulse, the probe-target complexes being configured to emit a photon of light when excited by current between the electrodes, and, a photosensor for detecting the light emitted by the probes, wherein during use, addition of the fluid to the probes prevents subsequent addition of other fluid to the probes. | 12-22-2011 |
20110312672 | MICROFLUIDIC ASSEMBLY WITH TEST MODULE AND DETACHABLE INDICATOR MODULE - A microfluidic assembly for detecting a target molecule in a sample fluid and indicating a positive or negative result, the microfluidic assembly having a test module having an outer casing with a receptacle for receiving the sample, functional sections for processing the sample and detecting the target molecules, a communication interface a controller for transmitting an output signal from one or more of the functional sections, and, an indicator module for detachable engagement with the communication interface, the indicator module having an indicator for providing an indication of the target molecule being present in the fluid sample. | 12-22-2011 |
20110312673 | DIALYSIS DEVICE WITH MULTI-LAYER STRUCTURE - A dialysis device for dialysis of a fluid containing constituents of different sizes, the dialysis device having a first layer of material defining a first channel and a second channel, the first channel configured for receiving the fluid which contains constituents of different sizes, a second layer having a plurality of apertures open to the first channel and at least one fluid connection leading from the apertures to the second channel for establishing fluid communication between the first channel and the second channel, wherein, the apertures are sized in accordance with a predetermined size threshold such that the constituents flowing to the second channel are smaller constituents that are smaller than the predetermined size threshold, and the constituents retained in the first channel include larger constituents which are larger than the predetermined size threshold. | 12-22-2011 |
20110312674 | LOC DEVICE WITH INTEGRAL PHOTOSENSOR FOR ELECTROCHEMILUMINESCENCE BASED DETECTION OF TARGETS - A lab-on-a-chip (LOC) device for detecting target molecules in a fluid, the LOC device having an array of electrochemiluminescent (ECL) probes for reaction with the target molecules to form probe-target complexes, electrodes for receiving an electrical pulse, the probe-target complexes being configured to emit a photon of light when excited by current between the electrodes, and, a photosensor for detecting light emitted by the probe-target complexes. | 12-22-2011 |
20110312675 | DIALYSIS DEVICE WITH FLOW-CHANNEL STRUCTURE FOR CAPILLARY-DRIVEN FLUIDIC PROPULSION WITHOUT TRAPPED AIR BUBBLES - A dialysis device with capillary-driven, flow-channel structure for dialysis of a fluid containing constituents of different sizes, the dialysis device having a first channel configured to fill with the fluid by capillary action, a second channel configured to fill with the fluid by capillary action, a plurality of fluid connections between the first channel and the second channel, each of the fluid connections being configured to pin a meniscus of the fluid that arrests capillary flow between the first channel and the second channel, a bypass channel between the first channel and the second channel, the bypass channel joining the second channel upstream of the plurality of fluid connections and is configured for uninterrupted capillary driven flow from the first channel to the second channel, wherein during use, flow from the bypass channel reaches the meniscus pinned at each of the fluid connections after the meniscus has formed such that the flow sequentially removes each of the menisci and sample flow from the first channel to the second channel is via the plurality of fluid connections as well as the bypass channel. | 12-22-2011 |
20110312676 | LOC DEVICE WITH INTEGRAL DRIVER FOR EXCITATION OF ELECTROCHEMILUMINESCENT LUMINOPHORES - A lab-on-a-chip (LOC) device for exciting electrochemiluminescent luminophores, the LOC device having a supporting substrate, an array of electrochemiluminescent (ECL) probes for reaction with the target molecules to form probe-target complexes, the probes each having a luminophore for emitting photons of light when in an excited state, electrodes for receiving an electrical pulse to excite the luminophores with current between the electrodes, and, CMOS circuitry for controlling the electrical pulse transmitted to the electrodes, wherein, the CMOS circuitry is between the supporting substrate and the array of probes. | 12-22-2011 |
20110312677 | LOC DEVICE FOR DETECTION OF TARGETS WITH ELECTROCHEMILUMINESCENT RESONANT ENERGY TRANSFER PROBES - A lab-on-a-chip (LOC) device for detecting target molecules in a fluid, the LOC device having an array of electrochemiluminescent (ECL) probes for reaction with the target molecules to form probe-target complexes, and, electrodes for receiving an electrical pulse, wherein, the probes each have an ECL luminophore that emits a photon when excited by current between the electrodes, and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer. | 12-22-2011 |
20110312678 | TEST MODULE WITH MICROFLUIDIC DEVICE HAVING DIALYSIS DEVICE, LOC AND INTERCONNECTING CAP - A test module for analyzing a sample fluid containing target molecules, the test module having an outer casing with a receptacle for receiving the sample fluid, and, a microfluidic device having: a dialysis device in fluid communication with the receptacle and configured to separate the target molecules from other constituents of the sample, a lab-on-a-chip (LOC) device for analyzing the target molecules, and, a cap overlaying the LOC device and the dialysis device for establishing fluid communication between the LOC device and the dialysis device. | 12-22-2011 |
20110312679 | MICROFLUIDIC DEVICE WITH SURFACE-MICROMACHINED DIALYSIS SECTION - A microfluidic device for removing cell debris from a biological sample, the microfluidic device having a dialysis section with a large constituent channel, a small constituent channel and a plurality of apertures for fluid communication between the large constituent channel and the small constituent channel, the large constituent channel having an upstream end for receiving the biological sample, the biological sample being a liquid carrying a mixture of cell debris and target molecules, the small constituent channel having a downstream end for connection to a hybridization section with an array of probes for reaction with the target molecules to form probe-target complexes, wherein, the apertures are spaced along the large constituent channel at a spacing between 1 micron and 10 microns and each of the apertures is sized to allow the target molecules to flow into the small constituent channel but retain the cell debris larger than a threshold size in the large constituent channel. | 12-22-2011 |
20110312680 | LOC DEVICE FOR DETECTING HYBRIDIZATION OF TARGET NUCLEIC ACID SEQUENCES WITH ELECTROCHEMILUMINESCENT RESONANT ENERGY TRANSFER, PRIMER-LINKED, LINEAR PROBES - A lab-on-a-chip (LOC) device for detecting hybridization of target nucleic acid sequences, the LOC device having electrochemiluminescent (ECL), resonant energy transfer, primer-linked, linear probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, each of the probes having a linear portion containing the target nucleic acid sequence, a primer for extension along the target nucleic acid sequence to form a nucleic acid sequence complementary to the target, an ECL luminophore for emitting photons when in an excited state, and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and, electrodes for receiving an electrical pulse to excite the ECL luminophores, wherein during use, replicating the target nucleic acid sequence causes the linear portion to dissociate from the functional moiety such that the complementary nucleic acid sequence therein hybridizes to the target nucleic acid sequence and photons emitted by the ECL luminophore are not quenched. | 12-22-2011 |
20110312681 | LOC DEVICE WITH DIALYSIS SECTION FOR REMOVING ERYTHROCYTES FROM BLOOD - A lab-on-a-chip (LOC) device for analyzing leukocytes in a blood sample, the LOC device having a supporting substrate, an inlet for receiving a sample of blood, a dialysis section for separating erythrocytes from leukocytes in the blood, and, a microsystems technology (MST) layer for analyzing the leukocytes, wherein, the inlet, the dialysis section and the MST layer are all supported on the supporting substrate. | 12-22-2011 |
20110312682 | LOC DEVICE FOR AMPLIFYING AND DETECTING TARGET NUCLEIC ACID SEQUENCES USING ELECTROCHEMILUMINESCENT RESONANT ENERGY TRANSFER, STEM-AND-LOOP PROBES WITH COVALENTLY ATTACHED PRIMERS - A lab-on-a-chip (LOC) device for amplifying and detecting target nucleic acid sequences, the LOC device having electrochemiluminescent (ECL), resonant energy transfer, stem-and-loop probes for hybridization with the target nucleic acid sequences, each of the probes having a loop portion containing a sequence complementary to the target nucleic acid sequence, an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and a covalently attached primer for extension along a complementary sequence denatured from the target nucleic acid sequence to replicate the target nucleic acid sequence, heaters for thermally cycling the target nucleic acid sequences through a polymerase chain reaction (PCR), in which the covalently attached primers anneal to oligonucleotides containing the target nucleic acid sequences, and, electrodes for receiving an electrical pulse to excite the ECL luminophores, wherein during use, replicating the target nucleic acid sequence causes the loop portion to open such the complementary nucleic acid sequence therein hybridizes to the target nucleic acid sequence and the ECL luminophore is moved away from the functional moiety. | 12-22-2011 |
20110312683 | MICROFLUIDIC TEST MODULE FOR INTERFACING WITH TABLET COMPUTER - A microfluidic test module for analyzing a sample fluid and communicating test results to a tablet computer, the microfluidic test module having an outer casing with a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the tablet computer, and, a controller for operative control of the communication interface. | 12-22-2011 |
20110312684 | LOC DEVICE FOR PATHOGEN DETECTION WITH DIALYSIS, LYSIS AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, and, a nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material, wherein, the dialysis section, the lysis section and the nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312685 | LOC DEVICE FOR PCR USING ADAPTOR PRIMERS AND TARGET DETECTION USING ELECTROCHEMILUMINESCENT RESONANT ENERGY TRANSFER PROBES - A lab-on-a-chip (LOC) device for amplifying and detecting target nucleic acid sequences, the LOC device having restriction enzymes for restricting double stranded oligonucleotides at known ligation sites, linker molecules for attaching to the ligated ends of the double stranded oligonucleotides, heaters for thermally cycling the oligonucleotides through a polymerase chain reaction (PCR) process, primers for annealing to the linker molecules on single strands of the oligonucleotides after denaturation, deoxyribonucleoside triphosphates (dNTPs) for extending the primers along the single strand oligonucleotides, and, electrodes for receiving an electrical pulse to excite the ECL luminophores. | 12-22-2011 |
20110312686 | MICROFLUIDIC DEVICE WITH ELONGATE PCR CHAMBERS - A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having at least one elongate PCR chamber having a longitudinal extent much greater than its lateral dimensions, and, at least one elongate heater element for heating the nucleic acid sequences within the elongate PCR chamber, wherein, the elongate heater element extends parallel with the longitudinal extent of the PCR chamber. | 12-22-2011 |
20110312687 | LOC DEVICE WITH LOW VOLUME HYBRIDIZATION CHAMBERS AND REAGENT RESERVOIRS FOR GENETIC ANALYSIS USING ELECTROCHEMILUMINESCENT TARGET DETECTION - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL), resonance energy transfer probes for hybridization with the target nucleic acid sequences, each of the probes containing a sequence complementary to the target nucleic acid sequence, and an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, hybridization chambers containing the probes for hybridization with the targets, at least one of the heaters and a pair of the electrodes, and, a reagent reservoir containing a reagent for addition to the fluid, wherein, the hybridization chambers each have a volume less than 900,000 cubic microns and the reagent reservoir has a volume less than 1,000,000,000 cubic microns. | 12-22-2011 |
20110312688 | MICROFLUIDIC DEVICE WITH PCR CHAMBER BETWEEN SUPPORTING SUBSTRATE AND HEATER - A microfluidic device having a supporting substrate, a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having a PCR chamber, and, a heater element for heating the nucleic acid sequences within the PCR chamber, wherein, the PCR chamber is between the heater element and the supporting substrate. | 12-22-2011 |
20110312689 | MICROFLUIDIC DEVICE WITH SENSOR-TRIGGERED PHOTODETECTION OF FLUORESCENT PROBE-TARGET HYBRID - A microfluidic device having a supporting substrate, an array of probes, each of the probes having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, CMOS circuitry on the substrate, the CMOS circuitry having an array of photodiodes for sensing deactivation of the excitation light and generating an output signal in response to the fluorescence signals from the probe-target hybrids within the array of probes. | 12-22-2011 |
20110312690 | MICROFLUIDIC DEVICE WITH PCR SECTION HAVING TWO-DIMENSIONAL CONTROL OF INPUT HEAT FLUX DENSITY - A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having a microchannel configured to have a plurality of mutually parallel, adjacent channel sections, and a plurality of elongate heaters positioned end to end along each of the channel sections, wherein, each of the heaters are independently operable for two-dimensional control of heat flux density to the PCR section. | 12-22-2011 |
20110312691 | LOC DEVICE WITH ELECTROCHEMILUMINESCENT PROBES INCLUDING POSITIVE AND NEGATIVE CONTROL PROBES - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, at least one positive control probe that has one of the ECL luminophores but not the functional moiety for quenching photon emission, at least one negative control probe that does not have one of the ECL luminophores, and, electrodes for receiving an electrical pulse to excite the ECL luminophores. | 12-22-2011 |
20110312692 | LOC DEVICE FOR PATHOGEN DETECTION WITH DIALYSIS, THERMAL LYSIS AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section having a lysis chamber and a heater for lysing the pathogens while the sample is in the lysis chamber, and, a nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material, wherein, the dialysis section, the lysis section and the nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312693 | MICROFLUIDIC DEVICE WITH FEEDBACK CONTROLLED PCR SECTION - A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having at least one heater, and, at least one sensor, wherein, the at least one sensor is configured to provide output for feedback control of the at least one heater. | 12-22-2011 |
20110312694 | MICROFLUIDIC DEVICE WITH DELAY-TRIGGERED PHOTODETECTION OF FLUORESCENT PROBE-TARGET HYBRID - A microfluidic device having a supporting substrate, a probe having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, CMOS circuitry on the substrate, the CMOS circuitry having a photosensor for generating an output signal in response to the fluorescence signal, wherein, the CMOS circuitry is configured to initiate a time delay upon deactivation of the excitation light before activating the photosensor. | 12-22-2011 |
20110312695 | LOC DEVICE WITH HYBRIDIZATION CHAMBER ARRAY WITH POSITIVE CONTROL CHAMBER CONTAINING ELECTROCHEMILUMINESCENT REPORTER - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, hybridization chambers containing the probes for detection of the targets, and a pair of the electrodes, and, at least one positive control chamber containing positive control probes that have the ECL luminophore but not the functional moiety for quenching photon emission. | 12-22-2011 |
20110312696 | LOC DEVICE FOR PATHOGEN DETECTION WITH DIALYSIS, CHEMICAL LYSIS AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the cells in the lysis section, and, a nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material, wherein, the dialysis section, the lysis section and the nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312697 | MICROFLUIDIC DEVICE WITH TEMPERATURE FEEDBACK CONTROLLED PCR SECTION - A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having at least one heater, and, at least one temperature sensor, wherein, the at least one temperature sensor is configured to provide output for feedback control of the at least one heater. | 12-22-2011 |
20110312698 | MICROFLUIDIC DEVICE WITH PCR SECTION HAVING SHORT THERMAL CYCLE TIMES - A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having at least one heater for thermally cycling the nucleic acid sequences through a denaturation phase, an annealing phase and a primer extension phase, wherein during use, the PCR section has a thermal cycle time of less than 30 seconds. | 12-22-2011 |
20110312699 | MICROFLUIDIC DEVICE WITH ON-CHIP SEMICONDUCTOR CONTROLLED PCR SECTION - A microfluidic device having a supporting substrate, a sample inlet for receiving a sample of biological material having nucleic acid sequences, a microsystems technology (MST) layer having a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having at least one heater for thermally cycling the nucleic acid sequences through a denaturation phase, an annealing phase and a primer extension phase, and, CMOS circuitry between the MST layer and the supporting substrate, the CMOS circuitry being connected to the at least one heater for operative control of the at least one heater during the thermal cycling. | 12-22-2011 |
20110312700 | MICROFLUIDIC DEVICE WITH PWM CONTROLLED PCR HEATER - A microfluidic device having a supporting substrate, a sample inlet for receiving a sample of biological material having nucleic acid sequences, a microsystems technology (MST) layer having a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having at least one heater for thermally cycling the nucleic acid sequences through a denaturing phase, an annealing phase and a primer extension phase, and, CMOS circuitry between the MST layer and the supporting substrate, the CMOS circuitry configured to energize the at least one heater with a pulse width modulated (PWM) signal during the thermal cycling. | 12-22-2011 |
20110312701 | LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET NUCLEIC ACID SEQUENCES WITH CALIBRATED PHOTODETECTION OF PROBES IN HYBRIDIZATION ARRAY - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, detection photodiodes for exposure to the photons emitted by the ECL luminophores, and, at least one calibration photodiode for exposure to ambient light, wherein during use, a difference between the output from any of the detection photodiode and the output from the calibration photodiode is compared to a predetermined threshold difference such that output differences greater than the predetermined threshold indicate the target is present. | 12-22-2011 |
20110312702 | LOC DEVICE FOR GENETIC ANALYSIS WITH DIALYSIS, CHEMICAL LYSIS AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating cells larger than a predetermined threshold in the sample from smaller constituents, whereby the cells larger than a predetermined threshold include target cells containing genetic material for analysis, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the target cells to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the target cells in the lysis section, and, a nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences from the genetic material, wherein, the dialysis section, the lysis section and the nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312703 | MICROFLUIDIC DEVICE FOR RAPID PCR AMPLIFICATION - A microfluidic device having a supporting substrate, a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having a PCR chamber, and, a heater element for heating the nucleic acid sequences within the PCR chamber, wherein during use, the PCR section generates sufficient amplicon for hybridization with an array of probes in less than 10 minutes. | 12-22-2011 |
20110312704 | MICROFLUIDIC DEVICE FOR PCR AMPLIFICATION USING LOW PCR MIXTURE VOLUME - A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section with a PCR microchannel for thermally cycling a PCR mixture of the sample, polymerase, dNTP's and buffer solution to amplify the nucleic acid sequences, the PCR microchannel defining a flow-path for the sample such that flow of the sample along the PCR microchannel is driven by capillary action, wherein, the PCR mixture has a volume less than 400 nanoliters. | 12-22-2011 |
20110312705 | TEST MODULE FOR PCR AMPLIFICATION USING LOW PCR MIXTURE VOLUME - A test module for amplifying nucleic acid sequences in a sample fluid, the test module having an outer casing with receptacle for receiving the sample fluid, a polymerase chain reaction (PCR) section with a PCR microchannel for thermally cycling a PCR mixture of the sample fluid, polymerase, dNTP's, primers, and buffer solution to amplify the nucleic acid sequences, wherein, the PCR mixture has a volume less than 400 nanoliters. | 12-22-2011 |
20110312706 | LOC DEVICE WITH HYBRIDIZATION CHAMBERS CONTAINING PROBES FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET NUCLEIC ACID SEQUENCES IN A FLUID AND CALIBRATION CHAMBER CONTAINING PROBES SEALED FROM THE FLUID - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having hybridization chambers containing electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the ECL probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, at least one calibration chamber containing probes sealed from contact with the fluid, and, electrodes for receiving an electrical pulse to excite the ECL luminophores. | 12-22-2011 |
20110312707 | GENETIC ANALYSIS LOC FOR PCR AMPLIFICATION OF NUCLEIC ACIDS - A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing buffer, dNTPs, primers, and polymerase for addition to the sample, and, a polymerase chain reaction (PCR) section for thermal cycling the sample, buffer, dNTPs, primers, and polymerase to amplify the target nucleic acid sequences. | 12-22-2011 |
20110312708 | GENETIC ANALYSIS LOC FOR ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS - A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing buffer, dNTPs, primers, and polymerase for addition to the sample to form an amplification mix, and, a nucleic acid amplification section for controlling the temperature of the amplification mix during isothermal amplification of the target nucleic acid sequences. | 12-22-2011 |
20110312709 | LOC DEVICE FOR DETECTING TARGET NUCLEIC ACID SEQUENCES USING ELECTROCHEMILUMINESCENT PROBES AND CALIBRATION PROBES WITH DETECTION PHOTOSENSORS AND CALIBRATION PHOTOSENSORS - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the ECL probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, at least one calibration source configured to generate a calibration emission, electrodes for receiving an electrical pulse to excite the ECL luminophores, at least one detection photodiode for sensing photon emissions from the ECL luminophores and generating a detection output, at least one calibration photodiode for sensing the calibration emission and generating a calibration output, and, CMOS circuitry having a differential circuit for subtracting the calibration output from the detection output. | 12-22-2011 |
20110312710 | LOC DEVICE FOR PATHOGEN DETECTION, GENETIC ANALYSIS AND PROTEOMIC ANALYSIS WITH DIALYSIS, CHEMICAL LYSIS, INCUBATION AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for pathogen detection, genetic analysis and proteomic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a leukocyte dialysis section for dividing the sample into a leukocyte stream, and a pathogen and erythrocyte stream containing cells and sample constituents smaller than a first size threshold, a pathogen dialysis section for dividing the pathogen and erythrocyte stream into an erythrocyte stream and a pathogen stream containing cells and sample constituents smaller than a second size threshold, a leukocyte lysis section downstream of the leukocyte dialysis section for lysing the leukocytes with a lysis reagent to release genetic material and proteins therein, a leukocyte incubation section downstream of the leukocyte lysis section for enzymatic reaction of the genetic material with enzymes, a leukocyte nucleic acid amplification section downstream of the leukocyte incubation section for amplifying nucleic acid sequences, a pathogen lysis section downstream of the pathogen dialysis section for lysing the pathogens with a lysis reagent to release genetic material and proteins therein, a pathogen incubation section downstream of the pathogen lysis section for enzymatic reaction of the genetic material with enzymes, a pathogen nucleic acid amplification section downstream of the pathogen incubation section for amplifying nucleic acid sequences, an erythrocyte lysis section downstream of the pathogen dialysis section for lysing the erythrocytes with a lysis reagent to release proteins therein, wherein, the leukocyte dialysis section, the pathogen dialysis section, the leukocyte lysis section, the pathogen lysis section, the erythrocyte lysis section, the leukocyte incubation section, the pathogen incubation section, the leukocyte nucleic acid amplification section and the pathogen nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312711 | MICROFLUIDIC DEVICE WITH CONTROLLABLE SHUNTS PERIPHERAL TO INTEGRATED PHOTODIODES - A microfluidic device having a probe for hybridization with a target nucleic acid sequence, a photodiode for detecting fluorescence emissions generated by hybridization of the probe in response to an excitation light, and, a shunt transistor between the photodiode anode and a voltage source, the shunt transistor being configured to remove carriers generated by absorption of photons of the excitation light in the photodiode, wherein, a peripheral edge of the photodiode and a peripheral edge of the shunt transistor contiguously abut each other. | 12-22-2011 |
20110312712 | GENETIC ANALYSIS LOC FOR PCR AMPLIFICATION OF NUCLEIC ACIDS FROM WHOLE BLOOD - A lab-on-a-chip (LOC) device for genetic analysis of a whole blood sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the whole blood sample, a plurality of reagent reservoirs containing buffer, dNTPs, primers, and polymerase for addition to the whole blood sample, and, a polymerase chain reaction (PCR) section for thermal cycling the whole blood sample, buffer, dNTPs, primers, and polymerase to amplify the target nucleic acid sequences. | 12-22-2011 |
20110312713 | MICROFLUIDIC TEST MODULE WITH LOW-VOLUME HYBRIDIZATION CHAMBERS FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET NUCLEIC ACID SEQUENCES IN A FLUID - A microfluidic test module for detecting target nucleic acid sequences in a fluid, the test module having an outer casing configured for hand-held portability, the outer casing having an inlet for receiving the fluid containing the target nucleic acid sequences, a hybridization chamber mounted in the casing, the hybridization chamber containing electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the ECL probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and electrodes for receiving an electrical pulse to excite the ECL luminophores, wherein, the hybridization chamber has a volume less than 900,000 cubic microns. | 12-22-2011 |
20110312714 | GENETIC ANALYSIS LOC FOR AMPLIFICATION OF NUCLEIC ACIDS USING DNA POLYMERASES OF THERMOPHILES - A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing buffer, dNTPs, primers, and polymerase derived from a thermophilic bacterium for addition to the sample to form an amplification mixture, and, a nucleic acid amplification section for controlling the temperature of the amplification mixture during amplification of the target nucleic acid sequences. | 12-22-2011 |
20110312715 | MICROFLUIDIC TEST MODULE WITH LOW MASS ELECTROCHEMILUMINESCENT PROBE SPOTS - A microfluidic test module for detecting target nucleic acid sequences in a fluid, the test module having an outer casing having an inlet for receiving the fluid containing the target nucleic acid sequences, electrode pairs for receiving an electrical pulse, electrochemiluminescent (ECL) probe spots in contact with each of the electrode pairs respectively, the ECL probe spots containing ECL probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, such that the electrical pulse to the electrode pair excites the ECL luminophores, wherein, the mass of the ECL probes in each of the probe spots is less than 270 picograms. | 12-22-2011 |
20110312716 | GENETIC ANALYSIS LOC FOR NUCLEIC ACID AMPLIFICATION USING AN ISOTHERMAL REACTION - A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing buffer solution, dNTPs, primers, and polymerase for addition to the sample to form an amplification mixture, and, a nucleic acid amplification section for maintaining the amplification mixture at a predetermined temperature during isothermal amplification of the target nucleic acid sequences. | 12-22-2011 |
20110312717 | LOC DEVICE FOR PATHOGEN DETECTION WITH DIALYSIS, INCUBATION AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a plurality of reagent reservoirs, a pathogen dialysis section for separating pathogens from constituents smaller than a predetermined threshold, a pathogen lysis section downstream of the pathogen dialysis section for lysing the pathogens with a lysis reagent to release genetic material therein, an incubation section downstream of the pathogen dialysis section, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, and, a nucleic acid amplification section downstream of the incubation section for amplifying nucleic acid sequences from the genetic material, wherein, the pathogen dialysis section, the pathogen lysis section, the incubation section and the nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312718 | GENETIC ANALYSIS LOC FOR NUCLEIC ACID AMPLIFICATION USING RECOMBINASE POLYMERASE AMPLIFICATION - A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing dNTPs, primers, recombinase, DNA polymerase and buffer solution for addition to the sample to form an amplification mixture, and, a nucleic acid amplification section for maintaining the amplification mixture at a predetermined temperature during recombinase polymerase amplification (RPA) of the target nucleic acid sequences. | 12-22-2011 |
20110312719 | TEST MODULE WITH INTEGRAL PHOTOSENSOR FOR ELECTROCHEMILUMINESCENT DETECTION OF HYBRIDIZATION - A test module for detecting target nucleic acid sequences in a fluid, the test module having an outer casing having a receptacle for receiving the fluid containing the target nucleic acid sequences, electrochemiluminescent (ECL) probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, and, a detection photosensor for exposure to the photons emitted by the ECL luminophores. | 12-22-2011 |
20110312720 | LOC DEVICE FOR GENETIC ANALYSIS WITH DIALYSIS AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating smaller constituents from larger constituents, the smaller constituents being smaller than a predetermined size threshold, and the larger constituents being larger than the predetermined size threshold, and, a nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material, wherein, the dialysis section and the nucleic acid amplification section are both supported on the supporting substrate. | 12-22-2011 |
20110312721 | LOC DEVICE FOR GENETIC ANALYSIS WITH DIALYSIS, INCUBATION, AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a plurality of reagent reservoirs, a dialysis section for separating cells larger than a predetermined threshold in the sample from smaller constituents, whereby the cells larger than a predetermined threshold include target cells containing genetic material for analysis, an incubation section downstream of the dialysis section, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, and, a nucleic acid amplification section downstream of the incubation section for amplifying nucleic acid sequences from the genetic material, wherein, the dialysis section, the incubation section and the nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312722 | PORTABLE TEST MODULE FOR EXCITATION OF ELECTROCHEMILUMINESCENT PROBES - A portable test module for excitation of electrochemiluminescent probes configured to detect target nucleic acid sequences, the test module having an outer casing configured for hand-held portability, the outer casing having a receptacle for receiving a fluid containing the target nucleic acid sequences, electrochemiluminescent (ECL) probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and, electrodes for receiving an electrical pulse to excite the ECL luminophores, wherein during use, the ECL probes that have detected one of the target nucleic acid sequences reconfigure such that the functional moiety does not quench the photon emission from the ECL luminophore when excited by the electrodes. | 12-22-2011 |
20110312723 | GENETIC ANALYSIS LOC FOR NUCLEIC ACID AMPLIFICATION USING NUCLEIC ACID SEQUENCE BASED AMPLIFICATION - A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing dNTPs, primers, reverse transcriptase, RNA polymerase, and buffer solution for addition to the sample to form an amplification mixture, and, a nucleic acid amplification section for maintaining the amplification mixture at a predetermined temperature during isothermal amplification of the target nucleic acid sequences. | 12-22-2011 |
20110312724 | LOC DEVICE FOR PATHOGEN DETECTION AND GENETIC ANALYSIS WITH INCUBATION, NUCLEIC ACID AMPLIFICATION AND PREHYBRIDIZATION FILTERING - A lab-on-a-chip (LOC) device for pathogen detection and genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample containing genetic material, a supporting substrate, a plurality of reagent reservoirs, an incubation section downstream of the inlet, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, and, a nucleic acid amplification section downstream of the incubation section for amplifying nucleic acid sequences in the genetic material, and, a dialysis section downstream of the nucleic acid amplification section for prehybridization filtration of amplicon produced by the nucleic acid amplification section, the dialysis section being configured to remove cell debris from the amplicon, wherein, the incubation section, the nucleic acid amplification section and the dialysis section are all supported on the supporting substrate. | 12-22-2011 |
20110312725 | USB-INTERFACEABLE PORTABLE TEST MODULE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGETS - A test module for excitation of electrochemiluminescent probes configured to detect target nucleic acid sequences, the test module having an outer casing having a receptacle for receiving a fluid containing the target nucleic acid sequences, electrochemiluminescent (ECL) probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, a detection photosensor for exposure to the photons emitted by the ECL luminophores, control circuitry providing the electrical pulse to the electrodes, and, a universal serial bus (USB) connection such that the outer casing is configured as a USB drive for transmitting data regarding detection of the targets in the fluid to an external device, wherein during use, the ECL probes that have detected one of the target nucleic acid sequences reconfigure such that the functional moiety does not quench the photon emission from the ECL luminophore when excited by the electrodes. | 12-22-2011 |
20110312726 | MICROFLUIDIC DEVICE WITH CONTROLLABLE SHUNTS INSIDE INTEGRATED PHOTODIODES - A microfluidic device having a probe for hybridization with a target nucleic acid sequence to form a probe-target hybrid, a photodiode having an active area for detecting fluorescence emissions generated by the probe-target hybrid in response to an excitation light, and, a shunt transistor between the photodiode anode and a voltage source, the shunt transistor being configured to remove carriers generated by absorption of photons of the excitation light in the photodiode, wherein, the shunt transistor is encompassed by the active area. | 12-22-2011 |
20110312727 | LOC DEVICE WITH PARALLEL NUCLEIC ACID AMPLIFICATION FUNCTIONALITY - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample containing genetic material, a supporting substrate, a plurality of reagent reservoirs, a first nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material in parallel with the first nucleic acid amplification section, wherein, the first nucleic acid amplification section and the second nucleic acid amplification section are both supported on the supporting substrate. | 12-22-2011 |
20110312728 | MICROFLUIDIC DEVICE WITH NON-IMAGING OPTICS - A microfluidic device having a supporting substrate, a hybridization chamber containing probes having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form probe-target hybrids, the probe-target hybrids being configured to generate a fluorescence signal in response to an excitation light, and, a photodiode with an active area and an optical axis extending normal to the active area and through the hybridization chamber, wherein, the active area is less than 249 microns from the probe-target hybrids. | 12-22-2011 |
20110312729 | TEST MODULE USING TRANSITION METAL-LIGAND COMPLEX, ELECTROCHEMILUMINESCENT LUMINOPHORES - A test module for excitation of electrochemiluminescent probes configured to detect target nucleic acid sequences, the test module having an outer casing with an inlet for receiving a fluid containing the target nucleic acid sequences, electrochemiluminescent (ECL) probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and, electrodes for receiving an electrical pulse to excite the ECL luminophores, such that the ECL probes that have detected one of the target nucleic acid sequences reconfigure such that the functional moiety does not quench the photon emission from the ECL luminophore when excited by the electrodes, wherein, the ECL luminophore has a transition metal-ligand complex. | 12-22-2011 |
20110312730 | LOC DEVICE WITH PARALLEL DNA AND RNA AMPLIFICATION FUNCTIONALITY - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample containing genetic material including DNA and RNA, a supporting substrate, a plurality of reagent reservoirs, a first nucleic acid amplification section for amplifying at least some of the genetic material, and, a second nucleic acid amplification section for amplifying at least some the genetic material in parallel with the first nucleic acid amplification section, wherein, the first nucleic acid amplification section and the second nucleic acid amplification section are both supported on the supporting substrate. | 12-22-2011 |
20110312731 | MICROFLUIDIC DEVICE WITH LARGE ANGLE OF COLLECTION OF EMISSION LIGHT - A microfluidic device having a supporting substrate, a hybridization chamber containing probes having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form probe-target hybrids and generate a fluorescence signal in response to an excitation light, and, a photodiode with an active area and an optical axis extending normal to the active area and through the hybridization chamber, wherein, the hybridization chamber has a floor surface positioned parallel to the active area of the photodiode, the floor surface having a centroid and the active area being encompassed within a cone having the centroid at its vertex, and a vertex angle less than 173°. | 12-22-2011 |
20110312732 | TEST MODULE USING LANTHANIDE METAL-LIGAND COMPLEX, ELECTROCHEMILUMINESCENT LUMINOPHORES - A test module for excitation of electrochemiluminescent probes configured to detect target nucleic acid sequences, the test module having an outer casing with an inlet for receiving a fluid containing the target nucleic acid sequences, electrochemiluminescent (ECL) probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and, electrodes for receiving an electrical pulse to excite the ECL luminophores, such that the ECL probes that have detected one of the target nucleic acid sequences reconfigure such that the functional moiety does not quench the photon emission from the ECL luminophore when excited by the electrodes, wherein, the ECL luminophore has a lanthanide metal-ligand complex. | 12-22-2011 |
20110312733 | LOC DEVICE WITH NUCLEIC ACID AMPLIFICATION SECTION - A lab-on-a-chip (LOC) device for amplifying nucleic acid sequences, the LOC device having an inlet for receiving a sample containing genetic material, a supporting substrate, a plurality of reagent reservoirs, and, a nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material, wherein, the nucleic acid amplification section is supported on the supporting substrate. | 12-22-2011 |
20110312734 | TEST MODULE WITH SUSPENDED ELECTROCHEMILUMINESCENT PROBES - A test module for detecting target nucleic acid sequences in a fluid, the test module having an outer casing having an inlet for receiving the fluid containing the target nucleic acid sequences, electrode pairs for receiving an electrical pulse, electrochemiluminescent (ECL) probes adjacent each of the electrode pairs respectively, each of the ECL probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, such that the electrical pulse to the electrode pair excites the ECL luminophores, wherein, the ECL probes are not anchored to a surface and form a suspension in the fluid containing the target nucleic acid sequences during detection. | 12-22-2011 |
20110312735 | MICROFLUIDIC DEVICE WITH NUCLEIC ACID AMPLIFICATION SECTION - A microfluidic device for amplifying nucleic acid sequences, the microfluidic device having an inlet for receiving a sample containing genetic material, a plurality of reagent reservoirs containing reagents for addition to the sample, and, a nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material. | 12-22-2011 |
20110312736 | MICROFLUIDIC DEVICE WITH FLOW RATE SENSOR - A microfluidic device for processing a fluid, the microfluidic device having an inlet for receiving the fluid, functional sections for processing the fluid, a flow-path extending from the inlet into at least one of the functional sections, a flow rate sensor with a conductive element positioned in proximity of the fluid flowing along the flow-path, CMOS circuitry configured for measuring the temperature of the conductive element, wherein, the CMOS circuitry is configured to provide a predetermined current through the conductive element and measure the electrical resistance of the conductive element such that a flow speed is derived from the current, the temperature sensor output and the electrical resistance, and a flow rate is derived using the flow speed and the flow-path cross section transverse to the flow direction at the conductive element. | 12-22-2011 |
20110312737 | SINGLE-USE TEST MODULE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGETS - A test module for detecting target nucleic acid sequences in a fluid, the test module having an outer casing with an inlet for receiving a fluid containing the target nucleic acid sequences, an array of electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, and, electrodes positioned for receiving an electrical pulse, the probe-target hybrids being configured to emit a photon of light when excited by current between the electrodes, and, a photosensor for detecting the light emitted by the probes, wherein during use, addition of the fluid to the probes prevents subsequent addition of other fluid to the probes. | 12-22-2011 |
20110312738 | MICROFLUIDIC DEVICE WITH LIQUID SENSOR - A microfluidic device for processing a fluid, the microfluidic device having an inlet for receiving the fluid, functional sections for processing the fluid, a flow-path extending from the inlet into at least some of the functional sections, circuitry for operative control of the functional sections, and, a liquid sensor with electrodes positioned for contact with the fluid flowing along the flow-path, wherein, the circuitry is configured to provide a voltage across the electrodes such that current above a predetermined threshold is indicative of liquid at the electrodes. | 12-22-2011 |
20110312739 | SINGLE-USE TEST MODULE FOR PCR AMPLIFICATION OF TARGETS AND ELECTROCHEMILUMINESCENT DETECTION OF TARGETS - A test module for amplifying and detecting target nucleic acid sequences in a fluid, the test module having an outer casing with a receptacle for receiving a fluid containing the target nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the target nucleic acid sequences, an array of electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, and, electrodes positioned for receiving an electrical pulse, the probe-target hybrids being configured to emit a photon of light when excited by current between the electrodes, and, a photosensor for detecting the light emitted by the probes, wherein during use, addition of the fluid to the probes prevents subsequent addition of other fluid to the probes. | 12-22-2011 |
20110312740 | MICROFLUIDIC DEVICE WITH CAPILLARY MENISCUS MARCHING VELOCITY SENSOR - A microfluidic device for processing a fluid, the microfluidic device having an inlet for receiving the fluid, functional sections for processing the fluid, a flow-path extending from the inlet into at least some of the functional sections, circuitry for operative control of the functional sections, and, a capillary meniscus marching velocity sensor having a plurality of liquid sensors spaced along the flow-path, each of the liquid sensors having electrodes positioned for contact with the fluid flowing along the flow-path, wherein, the circuitry is configured to provide a voltage across the electrodes such that current above a predetermined threshold is indicative of liquid at the electrodes and used to derive a velocity of the liquid flow front. | 12-22-2011 |
20110312741 | MICROFLUIDIC DEVICE FOR ANALYSIS OF MITOCHONDRIAL DNA - A microfluidic device for analysis of mitochondrial DNA in a sample, the microfluidic device having an inlet for receiving a sample of biological material having cells with an outer cellular membrane containing mitochondria and a nucleus, the mitochondria having a mitochondrial membrane containing mitochondrial DNA and the nuclei having a nuclear membrane containing nuclear DNA, a lysis section for lysing the outer cellular membrane and the mitochondrial membrane while the nuclear membrane remains intact, and, probes for hybridization with target nucleic acid sequences in the mitochondrial DNA to form probe-target hybrids. | 12-22-2011 |
20110312742 | SINGLE-USE MICROFLUIDIC DEVICE - A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing a target nucleic acid sequence, a probe with a nucleic acid sequence for hybridization with the target nucleic acid sequence to form a probe-target hybrid and generate a fluorescence signal in response to an excitation light, and, a photosensor for sensing the fluorescence signal, wherein during use, addition of the biological sample to the inlet prevents fluid communication between a fluid subsequently added to the inlet and the probe. | 12-22-2011 |
20110312743 | LOC DEVICE FOR DETECTION OF TARGET NUCLEIC ACID SEQUENCES USING ELECTRODES CONFIGURED FOR ELECTROCHEMILUMINESCENCE OF LUMINOPHORES WITHOUT A COREACTANT - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and, electrodes for receiving an electrical pulse to excite the ECL luminophores, wherein, the electrodes are arranged into pairs, each having an anode and a cathode, the anode and the cathode being separated by a dielectric gap between 0.4 microns and 2 microns wide. | 12-22-2011 |
20110312744 | MICROFLUIDIC DEVICE FOR AMPLIFYING MITOCHONDRIAL DNA IN A BIOLOGICAL SAMPLE - A microfluidic device for amplifying mitochondrial DNA in a sample, the microfluidic device having an inlet for receiving a sample of biological material having cells with an outer cellular membrane containing mitochondria and a nucleus, the mitochondria having a mitochondrial membrane containing mitochondrial DNA and the nuclei having a nuclear membrane containing nuclear DNA, a lysis section for lysing the outer cellular membrane and the mitochondrial membrane while the nuclear membrane remains intact, and, a nucleic acid amplification section configured for amplifying target nucleic acid sequences in the mitochondrial DNA. | 12-22-2011 |
20110312745 | MICROFLUIDIC TEST MODULE WITH PHOTOSENSOR - A test module for analyzing a sample fluid containing target molecules, the test module having an outer casing with a receptacle for receiving the sample fluid, probes for reaction with the target molecules to form probe-target complexes, the probe-target complexes being configured to emit photons when excited, and, a photosensor for detecting photons emitted by the probe-target complexes. | 12-22-2011 |
20110312746 | MICROFLUIDIC DEVICE WITH CHEMICAL LYSIS SECTION - A microfluidic device for lysing cells in a fluid, the microfluidic device having an inlet for receiving a fluid containing cells, a lysis reagent reservoir containing a lysis reagent and having an outlet valve, and, a lysis section in fluid communication with the inlet for holding the fluid during lysis of the cells, wherein, the outlet valve is upstream of the lysis section and configured to open as the fluid flows into the lysis section thereby adding the lysis reagent to the fluid. | 12-22-2011 |
20110312747 | MICROFLUIDIC DEVICE FOR BIOCHEMICAL PROCESSING AND ANALYSIS - A microfluidic device for biochemical processing and analysis, the microfluidic device having a supporting substrate, a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving biochemical liquid, and, a reagent reservoir containing a reagent for addition to the biochemical liquid for use in chemical analysis of the biochemical liquid, wherein, the reagent reservoir has an outlet with a surface tension valve for retaining the reagent in the reservoir with a reagent meniscus until contact with the biochemical liquid removes the reagent meniscus. | 12-22-2011 |
20110312748 | LOC WITH INTEGRAL PHOTOSENSOR FOR DETECTION OF HYBRIDIZATION ASSAY RESULTS - A microfluidic device having a supporting substrate, an array of probes for hybridization with a target nucleic acid sequence to form a probe-target hybrid and generate a fluorescence signal in response to an excitation light, and, a photosensor positioned adjacent the array of probes, wherein, the photosensor is configured for sensing which probes within the array of probes generated the fluorescence signal. | 12-22-2011 |
20110312749 | MICROFLUIDIC DEVICE WITH THERMAL LYSIS SECTION - A microfluidic device for lysing cells in a fluid, the microfluidic device having an inlet channel for receiving a fluid containing cells, a lysis section in fluid communication with the inlet channel for holding the fluid during lysis of the cells, and, a lysis heater for heating the fluid in the lysis section such that the cells lyse, wherein, the inlet channel is configured for filling the lysis section by capillary action. | 12-22-2011 |
20110312750 | MICROFLUIDIC DEVICE WITH TOTAL REAGENT STORAGE - A microfluidic device for biochemical processing and analysis, the microfluidic device having a supporting substrate, a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving biochemical liquid, and, a plurality of reagent reservoirs containing all reagents necessary for the biochemical processing, wherein, each of the reagent reservoirs have an outlet with a surface tension valve for retaining the reagent in the reservoir with a reagent meniscus until contact with the biochemical liquid removes the reagent meniscus. | 12-22-2011 |
20110312751 | MICROFLUIDIC DEVICE FOR DETECTION OF MITOCHONDRIAL DNA VIA FLUORESCENCE MODULATED BY HYBRIDIZATION - A microfluidic device for analysis of mitochondrial DNA in a sample, the microfluidic device having an inlet for receiving a sample of biological material having cells with mitochondria containing mitochondrial DNA, a lysis section for lysing the mitochondria to release the mitochondrial DNA, fluorescence resonance energy transfer (FRET) probes for hybridization with target nucleic acid sequences in the mitochondrial DNA to form probe-target hybrids, and, a photosensor for detecting the probe-target hybrids. | 12-22-2011 |
20110312752 | MICROFLUIDIC DEVICE WITH LOW-VOLUME REAGENT RESERVOIR - A microfluidic device for biochemical processing and analysis, the microfluidic device having a supporting substrate, a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving biochemical liquid, and, a reagent reservoir containing a reagent necessary for the biochemical processing, wherein, the reagent reservoir has a volume less than 1000,000,000 cubic microns. | 12-22-2011 |
20110312753 | LOC WITH INTEGRAL LED DRIVER FOR EXCITATION LED - A microfluidic device having a supporting substrate, a probe with a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid and generate a fluorescence signal in response to an excitation light, and, CMOS circuitry on the supporting substrate for operative control of the excitation light. | 12-22-2011 |
20110312754 | MICROFLUIDIC DEVICE FOR DETECTION OF MITOCHONDRIAL DNA VIA ELECTROCHEMILUMINESCENCE MODULATED HYBRIDIZATION - A microfluidic device for analysis of mitochondrial DNA in a sample, the microfluidic device having an inlet for receiving a sample of biological material having cells with mitochondria containing mitochondrial DNA, a lysis section for lysing the mitochondria to release the mitochondrial DNA, electrochemiluminescent (ECL) resonance energy transfer probes for hybridization with target nucleic acid sequences in the mitochondrial DNA to form probe-target hybrids, and, a photosensor for detecting the probe-target hybrids. | 12-22-2011 |
20110312755 | TEST MODULE WITH CHEMICAL LYSIS SECTION - A test module for lysing cells in a fluid, the test module having an outer casing with receptacle for receiving a fluid containing cells, a lysis reagent reservoir containing a lysis reagent and having an outlet valve, and, a lysis section in fluid communication with the receptacle for holding the fluid during lysis of the cells, wherein, the outlet valve is upstream of the lysis section and configured to open as the fluid flows into the lysis section thereby adding the lysis reagent to the fluid. | 12-22-2011 |
20110312756 | MICROFLUIDIC DEVICE WITH LOW REAGENT VOLUMES - A microfluidic device for biochemical processing and analysis, the microfluidic device having a supporting substrate, a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving biochemical liquid, and, a reagent reservoir containing a reagent necessary for the biochemical processing, wherein, the reagent reservoir contains less than 1,000,000,000 cubic microns of reagent. | 12-22-2011 |
20110312757 | REAGENT MICROVIAL WITH DIGITAL MEMORY - A reagent microvial for a reagent dispensing apparatus, the microvial having a container for holding a volume of reagent, a digital memory for data relating to the reagent, a droplet generator for ejecting droplets of the reagent from the container, and, electrical contacts for connection to a control processor in the reagent dispensing apparatus to receive drive pulses for the droplet generator and provide the data to the control processor. | 12-22-2011 |
20110312758 | TEST MODULE WITH THERMAL LYSIS SECTION - A test module for lysing cells in a fluid, the test module having an outer casing with receptacle for receiving a sample that is a fluid containing cells, a lysis section in fluid communication with the receptacle for holding the fluid during lysis of the cells, and, a lysis heater for heating the fluid in the lysis section to lyse cells, wherein, the test module is configured for drawing the fluid from the receptacle to the lysis section by capillary action. | 12-22-2011 |
20110312759 | GENETIC ANALYSIS LOC WITH REAGENT RESERVOIR - A LOC device for performing a genetic diagnostic assay, the LOC device having a supporting substrate, a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving a biological sample containing target nucleic acid sequences, probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, a flow-path extending from the inlet to the probes, and, a reagent reservoir containing a reagent for addition to the sample in the flow-path upstream of the probes. | 12-22-2011 |
20110312760 | REAGENT MICROVIAL WITH AUTHENTICATION INTEGRATED CIRCUIT - A reagent microvial for a reagent dispensing apparatus, the microvial having a container for holding a volume of reagent, an integrated circuit with digital memory for data relating to an identity of the microvial, and, electrical contacts for connection to a control processor in the reagent dispensing apparatus to provide the data to the control processor for comparison with a list of authentic microvial identities. | 12-22-2011 |
20110312761 | TEST MODULE FOR CHEMICALLY AND THERMALLY LYSING CELLS - A test module for lysing cells in a fluid, the test module having an outer casing with receptacle for receiving a fluid containing cells, a lysis reagent reservoir containing a lysis reagent and having a valve, and, a lysis section in fluid communication with the receptacle for holding the fluid during lysis of the cells, the lysis section having a lysis heater for heating the fluid in the lysis section, wherein, the valve is upstream of the lysis section and configured to open as the fluid flows into the lysis section thereby adding the lysis reagent to the fluid. | 12-22-2011 |
20110312762 | LOC FOR DETECTION OF HYBRIDIZATION OF NUCLEIC ACID SEQUENCES WITH FLUORESCENCE RESONANCE ENERGY TRANSFER (FRET) PROBES - A lab-on-a-chip (LOC) device having a supporting substrate, a microsystems technologies (MST) layer with a hybridization section that has an array of fluorescence resonance energy transfer (FRET) probes for hybridization with target nucleic acid sequences in a fluid, and, a photosensor for detecting hybridization of probes within the array of probes. | 12-22-2011 |
20110312763 | GENETIC ANALYSIS LOC WITH IN-LOC STORAGE OF ALL REQUIRED REAGENTS - A lab-on-a-chip (LOC) device for genetic analysis having a supporting substrate, a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving a biological sample containing target nucleic acid sequences, probes for hybridization with the target nucleic acid sequence to form probe-target hybrids, and, a plurality of reagent reservoirs containing all reagents necessary for performing the diagnostic assay. | 12-22-2011 |
20110312764 | MICROFLUIDIC DEVICE WITH INCUBATOR - A microfluidic device having a supporting substrate, an inlet for receiving a fluid, a microsystems technology (MST) layer for processing the fluid, the MST layer incorporating an incubation section, the incubation section having at least one heater for maintaining the fluid at a predetermined incubation temperature, and, CMOS circuitry between the MST layer and the supporting substrate, the CMOS circuitry being connected to the at least one heater for operative control of the at least one heater. | 12-22-2011 |
20110312765 | GENETIC ANALYSIS LOC WITH LOW OLIGONUCLEOTIDE PROBE MASS AND LOW REAGENT VOLUME - A lab-on-a-chip (LOC) device for genetic analysis having a supporting substrate, a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving a biological sample containing target nucleic acid sequences, a hybridization section comprising an array of chambers with probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, and, a reagent reservoir containing a reagent necessary for processing the biological sample prior to hybridization, wherein, the hybridization section contains less than 270 picograms of each probe per chamber and the reagent reservoir containing less than 1000,000,000 cubic microns of the reagent. | 12-22-2011 |
20110312766 | MICROFLUIDIC DEVICE WITH FEEDBACK CONTROLLED INCUBATION SECTION - A microfluidic device having a sample inlet for receiving a fluid sample, an incubation section for maintaining the fluid sample at an incubation temperature, the incubation section having at least one heater, and, at least one sensor, wherein, the at least one sensor is configured to provide output for feedback control of the at least one heater. | 12-22-2011 |
20110312767 | MICROFLUIDIC DEVICE WITH INCUBATION SECTION HAVING TEMPERATURE FEEDBACK - A microfluidic device having a sample inlet for receiving a fluid sample, an incubation section for maintaining the fluid sample at an incubation temperature, the incubation section having at least one heater and at least one temperature sensor, wherein, the at least one temperature sensor is configured to provide output for feedback control of the at least one heater. | 12-22-2011 |
20110312768 | LOC FOR DETECTION OF HYBRIDIZATION OF NUCLEIC ACID SEQUENCES WITH PCR AMPLIFICATION USING PRIMERS COVALENTLY ATTACHED TO STEM-AND-LOOP PROBES - A LOC device having a supporting substrate, primer-linked, stem-and-loop probes, each with a nucleic acid sequence that matches one of a plurality of target nucleic acid sequences, and a primer for elongating against the target nucleic acid sequence to form a complementary sequence such that during use the nucleic acid sequence matching the target nucleic acid sequence anneals to the complementary sequence to change a fluorescence emission from the probe in response to an excitation light, a polymerase chain reaction (PCR) section for amplifying the target nucleic acid sequences in the fluid prior to hybridization with the FRET probes, and, CMOS circuitry on the supporting substrate, the CMOS circuitry having operative control of the excitation light. | 12-22-2011 |
20110312769 | TEST MODULE WITH LOW-VOLUME REAGENT RESERVOIR - A test module for biochemical processing and analysis, the test module having an outer casing dimensioned for hand-held portability, the outer casing having a receptacle for receiving biochemical liquid, and, a reagent reservoir containing a reagent for addition to the biochemical liquid for use in chemical analysis of the biochemical liquid, wherein, the reagent reservoir has a volume less than 1000,000,000 cubic microns. | 12-22-2011 |
20110312770 | LOC FOR DETECTION OF HYBRIDIZATION OF NUCLEIC ACID SEQUENCES WITH NUCLEIC ACID AMPLIFICATION USING PRIMERS COVALENTLY ATTACHED TO LINEAR PROBES - A LOC device having a supporting substrate, a primer-linked, linear probe with a nucleic acid sequence that matches a target nucleic acid sequence, and a primer for elongating against the target nucleic acid sequence to form a complementary sequence such that during use the nucleic acid sequence matching the target nucleic acid sequence anneals to the complementary sequence to change a fluorescence emission from the probe in response to an excitation light, and, CMOS circuitry on the supporting substrate, the CMOS circuitry having operative control of the excitation light. | 12-22-2011 |
20110312771 | MICROFLUIDIC DEVICE WITH PWM CONTROLLED INCUBATION SECTION - A microfluidic device having a sample inlet for receiving a fluid sample, an incubation section in fluid communication with the sample inlet, the incubation section having at least one heater for maintaining the fluid sample at an incubation temperature for an incubation period, and, CMOS circuitry to energize the at least one heater with a pulse width modulated (PWM) signal. | 12-22-2011 |
20110312772 | LOC FOR DETECTION OF HYBRIDIZATION OF NUCLEIC ACID SEQUENCES WITH PCR AMPLIFICATION USING LINKER PRIMERS - A LOC device for amplifying nucleic acid sequences and detecting target nucleic acid sequences, the LOC device having a supporting substrate, restriction enzymes for digesting double stranded nucleic acid sequences at known restriction sites, linker primers for ligation to the restricted ends of the double stranded nucleic acid sequences, heaters for thermally cycling the nucleic acid sequences through a polymerase chain reaction (PCR) process, deoxyribonucleoside triphosphates (dNTPs) for extending the linker primers along the nucleic acid sequences, and, probes for hybridizing with target nucleic acid sequences. | 12-22-2011 |
20110312773 | MICROFLUIDIC DEVICE WITH FAULT-TOLERANT MULTIPLE VALVE ASSEMBLY - A microfluidic device having an inlet for receiving a liquid flowing through the microfluidic device, an outlet downstream of the inlet, and, a fault-tolerant multiple valve assembly having a plurality of flow-paths extending from the inlet to the outlet and a plurality of valves positioned along each of the flow-paths respectively. | 12-22-2011 |
20110312774 | MICROFLUIDIC DEVICE FOR DIFFUSIVE MIXING IN SMALL CROSS SECTIONAL AREA MICROCHANNEL - A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer on the supporting substrate, the MST layer being configured to combine at least two liquids to form a flow of combined liquids, the MST layer having a diffusion mixing section for diffusive mixing of the at least two liquids, the mixing section having a microchannel defining a flow-path for the combined liquids, wherein, the channel cross section transverse to the flow direction is less than 100,000 square microns. | 12-22-2011 |
20110312775 | MICROFLUIDIC DEVICE WITH DIGITAL MEMORY - A microfluidic device for analyzing a sample fluid, the microfluidic device having a sample inlet for receiving the sample, a microsystems technology (MST) layer with functional sections for processing and analyzing the sample, and, CMOS circuitry with digital memory for storing data and operational information to operatively control the functional sections during processing and analysis of the sample. | 12-22-2011 |
20110312776 | GENETIC ANALYSIS LOC WITH HYBRIDIZATION ARRAY WITH POSITIVE CONTROL CHAMBERS INCORPORATING REPORTERS - A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing a target nucleic acid sequence, probes that each have a nucleic acid sequence for hybridization with the target nucleic acid sequence to form a probe-target hybrid, a fluorophore and a quencher configured such that the fluorophore emits a fluorescence signal in response to an excitation light and the quencher quenches the fluorescence signal when the probe is not hybridized, but fails to quench the fluorescence signal from the probe-target hybrid, and, a reporter with a fluorophore positioned for exposure to the excitation light simultaneously with the probes, wherein, the reporter always emits the fluorescence signal in response to the excitation light. | 12-22-2011 |
20110312777 | TEST MODULE WITH DIGITAL MEMORY - A test module for analyzing a sample fluid, the test module having a receptacle for receiving the sample, functional sections for processing and analyzing the sample, and, digital memory for storing data and operational information to operatively control the functional sections during processing and analysis of the sample. | 12-22-2011 |
20110312778 | GENETIC ANALYSIS LOC WITH HYBRIDIZATION ARRAY WITH NEGATIVE CONTROL CHAMBERS INCORPORATING PROBES DESIGNED TO BE NONCOMPLEMENTARY TO NUCLEIC ACID SEQUENCES IN THE AMPLICON - A lab-on-a-chip (LOC) device for genetic analysis having a supporting substrate, an inlet for receiving a biological sample containing target nucleic acid sequences, an array of probes each having a nucleic acid sequence for hybridization with the target nucleic acid sequences to form probe-target hybrids, and, a control probe designed to be complementary to sequences known to be absent from the biological sample. | 12-22-2011 |
20110312779 | OLIGONUCLEOTIDE SPOTTING DEVICE FOR OPERATION UNDER EXTERNAL MICROPROCESSOR CONTROL - An oligonucleotide spotting device for contactless spotting of probes onto a surface, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample, the oligonucleotide spotting device having a supporting substrate, an array of ejectors, each having an actuator for ejecting droplets of liquid containing the oligonucleotide probes, CMOS circuitry for providing each of the drop ejection actuators with a drive pulse for droplet ejection, and, bond-pads for electrically connecting the CMOS circuitry and an external microprocessor controller for operative control of the array of ejectors. | 12-22-2011 |
20110312780 | GENETIC ANALYSIS LOC WITH HYBRIDIZATION ARRAY WITH NEGATIVE CONTROL CHAMBERS INCORPORATING PROBES WITH NO REPORTERS - A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing target nucleic acid sequences, probes that each have a nucleic acid sequence for hybridization with the target nucleic acid sequences to form probe-target hybrids, a fluorophore and a quencher configured such that the fluorophore emits a fluorescence signal in response to an excitation light and the quencher quenches the fluorescence signal when the probe is not hybridized, but fails to quench the fluorescence signal from the probe-target hybrid, and, a control probe with no fluorophore such that the control probe never emits the fluorescence signal in response to the excitation light. | 12-22-2011 |
20110312781 | LOC WITH DIGITAL MEMORY TO STORE GENETIC DATA UPDATES - A lab-on-a-chip (LOC) device for analyzing a sample fluid, the LOC device having a sample inlet for receiving the sample, a microsystems technology (MST) layer with functional sections for processing and analyzing the sample, and, CMOS circuitry with digital memory for storing genetic data, and configured to download genetic data updates from an external source. | 12-22-2011 |
20110312782 | OLIGONUCLEOTIDE SPOTTING DEVICE WITH DIGITAL MEMORY - An oligonucleotide spotting device for contactless spotting of probes onto a surface, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample, the oligonucleotide spotting device having a supporting substrate, an array of ejectors, each having an actuator for ejecting droplets of liquid containing the oligonucleotide probes, and, CMOS circuitry for providing each of the drop ejection actuators with a drive pulse for droplet ejection, wherein, the CMOS circuitry has a digital memory for storing data related to the device. | 12-22-2011 |
20110312783 | GENETIC ANALYSIS LOC WITH HYBRIDIZATION ARRAY WITH NEGATIVE CONTROL COMPRISING EMPTY CHAMBERS - A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing target nucleic acid sequences, probes that each have a nucleic acid sequence for hybridization with a respective one of the target nucleic acid sequences to form a probe-target hybrid and emit a fluorescence signal in response to an excitation light, and, photosensors corresponding to each of the probes respectively, and, a control photosensor not corresponding to any of the probes such that the control photosensor does not sense the fluorescence signal of a corresponding probe-target hybrid during correct operation of the microfluidic device. | 12-22-2011 |
20110312784 | MICROFLUIDIC DEVICE FOR DETECTING TARGETS WITH PROBES, DETECTION PHOTODIODES AND A CALIBRATION PHOTODIODE - A microfluidic device for detecting target molecules in a sample, the microfluidic device having probes for reaction with the target molecules to form probe-target complexes, the probe-target complexes being configured to emit photons when excited, photodiodes for detecting photons emitted by the probe-target complexes and a calibration photodiode for exposure to a calibration source to generate a calibration signal, wherein during use, output from the photodiodes for detecting photons emitted by the probe-target complexes is calibrated by the calibration signal. | 12-22-2011 |
20110312785 | SPOTTING DEVICE FOR SPOTTING FIXED ARRAY OF LOCS - A spotting device for contactless spotting of lab-on-a-chip (LOC) devices with oligonucleotide probes, the LOC devices being held in a fixed array on a printed circuit board (PCB) and each having an array of hybridization chambers for receiving the oligonucleotide probes, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample, the spotting device having a monolithic supporting substrate, an array of reservoirs on one side of the supporting substrate, the reservoirs containing sufficient amount of the oligonucleotide probes suspended in a liquid to spot all the LOC devices on the PCB, and, an array of ejectors on the other side of the supporting substrate, each of the ejectors being configured for fluid communication with a corresponding one of the reservoirs respectively such that the ejectors eject droplets containing the oligonucleotide probes from the corresponding reservoir into one of the hybridization chambers. | 12-22-2011 |
20110312786 | GENETIC ANALYSIS LOC WITH HYBRIDIZATION ARRAY WITH CALIBRATION CHAMBER CONTAINING PROBE DESIGNED TO BE NONCOMPLEMENTARY TO NUCLEIC ACID SEQUENCES IN THE AMPLICON - A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing a target nucleic acid sequences, a hybridization chamber containing probes each having a nucleic acid sequence for hybridization with the target nucleic acid sequences to form probe-target hybrids, and, a calibration chamber containing a control probe incapable of hybridization with any sequence in the biological sample. | 12-22-2011 |
20110312787 | LOC HAVING USB DEVICE DRIVER FOR USE IN A TEST MODULE TO CONTROL USB CONNECTION - A lab-on-a-chip (LOC) device for installation in a test module for analyzing a sample fluid and communicating test results to an external device, the LOC device having a supporting substrate, a sample inlet for receiving the sample, a microsystems technology (MST) layer with functional sections for processing and analyzing the sample, and, CMOS circuitry on the supporting substrate, the CMOS circuitry having a universal serial bus (USB) device driver for operative control of a USB plug in the test module for communication with the external device. | 12-22-2011 |
20110312788 | GENETIC ANALYSIS LOC WITH THERMAL BOILING-INITIATED VALVE - A lab-on-a-chip (LOC) device for genetic analysis having a supporting substrate, an array of probes for hybridization with target nucleic acid sequences to form probe-target hybrids, a channel for directing a flow of liquid containing the target nucleic acid sequences, the channel having an inlet, an outlet and a meniscus anchor between the inlet and the outlet such that the flow from the inlet towards the outlet stops at the meniscus anchor where the liquid forms a meniscus, and, an actuator valve with a valve heater for heating the meniscus such that the meniscus unpins from the meniscus anchor such that the liquid flow towards the outlet resumes. | 12-22-2011 |
20110312789 | LOC DEVICE WITH FLASH MEMORY - A lab-on-a-chip (LOC) device for analyzing a sample fluid, the LOC device having a supporting substrate, a sample inlet for receiving the sample, a microsystems technology (MST) layer with functional sections for processing and analyzing the sample, and, CMOS circuitry between the supporting substrate and the MST layer, the CMOS circuitry having flash memory for storing program data to operate the functional sections during processing and analysis of the sample. | 12-22-2011 |
20110312790 | MICROFLUIDIC TEST MODULE WITH LOW-VOLUME HYBRIDIZATION CHAMBER - A microfluidic test module having an outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, and, a hybridization chamber mounted in the casing, the hybridization chamber containing probes having a nucleic acid sequence for hybridization with the target nucleic acid sequence to form probe-target hybrids, wherein, the hybridization chamber has a volume less than 900,000 cubic microns. | 12-22-2011 |
20110312791 | TEST MODULE WITH FAULT-TOLERANT MULTIPLE VALVE ASSEMBLY - A test module for genetic analysis having a casing for hand held portability, an inlet for receiving a liquid containing nucleic acid sequences, an outlet downstream of the inlet, and, a fault-tolerant multiple valve assembly having a plurality of flow-paths extending from the inlet to the outlet and a plurality of valves positioned along each of the flow-paths respectively. | 12-22-2011 |
20110312792 | TEST MODULE THAT UPDATES EPIDEMIOLOGICAL DATABASES - A test module for analyzing a sample fluid and communicating epidemiological data to a database, the test module having a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the epidemiological database, and, a controller for operative control of the communication interface. | 12-22-2011 |
20110312793 | MICROFLUIDIC TEST MODULE WITH LOW MASS OF PROBES - A microfluidic test module having an outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, and, a hybridization chamber mounted in the casing, the hybridization chamber holding a volume of fluid containing probes, the probes each having a nucleic acid sequence for hybridization with the target nucleic acid sequence to form probe-target hybrids, wherein, the mass of the probes in each of the hybridization chambers is less than 270 picograms. | 12-22-2011 |
20110312794 | TEST MODULE THAT UPDATES EPIDEMIOLOGICAL DATABASES WITH LOCATION DATA - A test module for analyzing a sample fluid and communicating location data to an epidemiological database, the test module having a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the epidemiological database, and, a controller for operative control of the communication interface, wherein, the controller is configured to associate location data with epidemiological data sent to the communication interface for communication with the epidemiological database. | 12-22-2011 |
20110312795 | DIAGNOSTIC TEST MODULE WITH A LOC WITH INTEGRAL PHOTOSENSOR AND EXCITATION LED FOR DETECTION OF HYBRIDIZATION ASSAY RESULTS - A test module for detecting hybridization of probes with target nucleic acid sequences, the test module having a receptacle for receiving a biological sample with target nucleic acid sequences, an array of probes for hybridization with the target nucleic acid sequences, an excitation light emitting diode (LED) for illuminating the array of probes, CMOS circuitry having a photosensor for sensing a fluorescence emission from any of the probes in the array that have hybridized with the target nucleic acid sequences such that the CMOS circuitry is configured to use outputs from the photosensor to generate a signal indicative of the probes that have hybridized, wherein, the array of probes and the CMOS circuitry are integrated on a lab-on-a-chip (LOC) device contained within the test module. | 12-22-2011 |
20110312796 | TEST MODULE THAT UPDATES MEDICAL DATABASES - A test module for analyzing a sample fluid and communicating data to a medical database, the test module having a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the medical database, and, a controller for operative control of the communication interface. | 12-22-2011 |
20110312797 | PORTABLE TEST MODULE FOR FLUORESCENCE EXCITATION OF PROBE NUCLEIC ACID SEQUENCES - A test module for detecting hybridization of probes with target nucleic acid sequences, the test module having a casing configured for hand held portability, the casing having a receptacle for receiving a biological sample with target nucleic acid sequences, an array of probes for hybridization with the target nucleic acid sequences, an excitation light source for illuminating the array of probes, and a photosensor for sensing a fluorescence emission from any of the probes in the array that have hybridized with the target nucleic acid sequences. | 12-22-2011 |
20110312798 | TEST MODULE WITH INBUILT LANCET - A test module for processing and analyzing a blood sample, the test module having an outer casing with a receptacle for receiving blood, functional sections for processing and analyzing the sample, and, a lancet for collecting the blood sample from a patient. | 12-22-2011 |
20110312799 | USB-INTERFACEABLE PORTABLE TEST MODULE FOR DETECTION OF HYBRIDIZED PROBES - A test module for detecting hybridization of probes with target nucleic acid sequences, the test module having a receptacle for receiving a biological sample with target nucleic acid sequences, an array of probes for hybridization with the target nucleic acid sequences, an excitation light source for illuminating the array of probes, circuitry incorporating a photosensor for sensing a fluorescence emission from any of the probes in the array that have hybridized with the target nucleic acid sequences, wherein, the circuitry is configured to use outputs from the photosensor to generate a signal indicative of the probes that have hybridized and transmit the signal to an external device. | 12-22-2011 |
20110312800 | TEST MODULE FOR GRAVITY-INDEPENDENT OPERATION - A test module for analyzing genetic material in a biological sample, the test module having an outer casing with a receptacle for receiving the biological sample, functional sections for processing and analyzing the biological sample, a controller for operating the functional sections, and, a flow-path from the receptacle to the functional sections, wherein, the flow-path is configured to draw the sample to be analyzed from the receptacle to the functional sections by capillary action regardless of module orientation relative to gravity. | 12-22-2011 |
20110312801 | TEST MODULE WITH LANTHANIDE METAL-LIGAND COMPLEX FLUOROPHORE - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, and, probes in the outer casing for hybridization with the target nucleic acid sequence to form probe-target hybrids, the probes each having a fluorophore such that the probe-target hybrids emit a fluorescence signal in response to exposure to an excitation light, wherein, the fluorophore is a lanthanide metal-ligand complex. | 12-22-2011 |
20110312802 | TEST MODULE WITH PROBES SUSPENDED IN FLUID - A test module having an outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, and, probes in the outer casing for hybridization with the target nucleic acid sequence to form probe-target hybrids, the probes each having a fluorophore such that the probe-target hybrids emit a fluorescence signal in response to exposure to an excitation light, wherein, the probes are suspended in a fluid. | 12-22-2011 |
20110312803 | SYSTEM FOR VARIABLE LOADING OF REAGENTS INTO MICROFLUIDIC DEVICE FOR GENETIC ANALYSIS - A system for loading reagents into a microfluidic device for genetic analysis, the system having containers with reagents for processing a biological sample in the microfluidic device, an integrated circuit secured to each of the containers respectively, each of the integrated circuits having a container digital memory storing reagent specification data related to the reagent in that container, a microfluidic device for performing a desired genetic test assay, the microfluidic device having a device digital memory, a reagent dispensing apparatus for loading a selection of the reagents into the microfluidic device, the reagent dispensing apparatus having a control microprocessor for accessing the container digital memory and transmitting the specification data to the device digital memory, and, a device reader for accessing the reagent specification data from the device digital memory in order to analyze test assay results from the microfluidic device. | 12-22-2011 |
20110312804 | MICROFLUIDIC DEVICE WITH APERTURE WITH GEOMETRY TO PROMOTE UNPINNED FLOW-THROUGH OF FLUID - A microfluidic device having a channel having an inlet, an outlet and an aperture between the inlet and the outlet, wherein, the aperture has a geometry that does not allow a meniscus of the fluid flow to anchor to the aperture, such that fluid flow from the inlet towards the outlet is uninterrupted. | 12-22-2011 |
20110312805 | TEST MODULE WITH TIME DELAYED DETECTION OF FLUORESCENCE FROM HYBRIDIZED PROBE - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, control circuitry having a photosensor for generating an output signal in response to the fluorescence signal, wherein, the control circuitry is configured to initiate a time delay upon deactivation of the excitation light before activating the photosensor. | 12-22-2011 |
20110312806 | MICROFLUIDIC DEVICE WITH HUMIDIFIER - A microfluidic device for analyzing a fluid sample containing target molecules, the microfluidic device having a supporting substrate, a microsystems technologies (MST) layer on the supporting substrate for processing the fluid sample, the MST layer having an array of probes configured to combine with the target molecules to form probe-target complexes, and, a humidifier configured to heat water for localized humidification of an area encompassing the MST layer. | 12-22-2011 |
20110312807 | MICROFLUIDIC TEST MODULE WITH A MEMBRANE SEAL TO PREVENT DEHUMIDIFICATION OF THE MIXTURE - A microfluidic test module for analyzing genetic material, the test module having a casing configured for hand-held portability, an array of probes housed in the casing, the probes being configured to hybridize with target nucleic acid sequences to form probe-target hybrids, wherein, the casing has a membrane seal for reducing dehumidification within the casing and providing pressure relief from small air pressure fluctuations. | 12-22-2011 |
20110312808 | TEST MODULE WITH CONTROLLED EXPOSURE OF FLUOROPHORES TO EXCITATION LIGHT SOURCE - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, and, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, control circuitry having a photosensor for generating an output signal in response to the fluorescence signal, wherein, the control circuitry is configured to initiate a time delay upon deactivation of the excitation light before activating the photosensor. | 12-22-2011 |
20110312809 | TEST MODULE WITH HUMIDIFIER - A test module for analyzing genetic material, the test module having a casing configured for hand-held portability, an array of probes housed in the casing, the probes being configured to hybridize with target nucleic acid sequences to form probe-target hybrids, and, a humidifier for increasing the humidity within the casing. | 12-22-2011 |
20110312810 | SINGLE-USE TEST MODULE FOR DETECTION OF HYBRIDIZATION OF TARGETS WITH OLIGONUCLEOTIDE PROBES - A single-use test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, a photosensor to detect the fluorescence signal. | 12-22-2011 |
20110312811 | LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET SEQUENCES WITH ELECTRODES PROFILED FOR GREATER PERIPHERAL EDGE LENGTH - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, a photosensor for sensing the photons emitted from the ECL luminophore, wherein, the electrodes are plates of conductive material, the plates having edge profiles configured such that the length of peripheral edge of each of the plates is greater than 128 microns. | 12-22-2011 |
20110312812 | GENETIC TEST MODULE WITH FEEDBACK-CONTROLLED HUMIDIFIER - A test module for processing genetic material in a biological sample, the test module having a casing, a microfluidic device housed in the casing, the microfluidic device having reagents for processing the genetic material, a humidifier for increasing the humidity within the casing, a humidity sensor for sensing the humidity within the casing, wherein, the humidity sensor generates an output used for control of the humidifier. | 12-22-2011 |
20110312813 | SINGLE-USE GENETIC TEST MODULE - A single-use test module for detecting hybridization of probes with nucleic acid sequences, the test module having a sample input for receiving a biological sample with target nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the target nucleic acid sequences in the biological sample, an array of probes for hybridization with target nucleic acid sequences in the biological sample, and, circuitry having a photosensor for sensing hybridization between the probes and target nucleic acid sequences in the biological sample, wherein, the circuitry is configured to use outputs from the photosensor to generate a signal indicative of the probes that have hybridized. | 12-22-2011 |
20110312814 | SINGLE-USE TEST MODULE WITH EXCITATION SOURCE - A single-use test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, an excitation light source. | 12-22-2011 |
20110312815 | MICROFLUIDIC DEVICE WITH HUMIDITY SENSOR - A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer on the supporting substrate for processing a fluid sample, and, a humidity sensor for sensing the humidity of an area encompassing the MST layer, wherein, the humidity sensor generates an output for humidity control of the area encompassing the MST layer. | 12-22-2011 |
20110312816 | TEST MODULE WITH LED FOR SIMULTANEOUS EXCITATION OF OLIGONUCLEOUTIDE PROBES - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, an excitation LED for generating the excitation light, the LED positioned in the outer casing for simultaneously illuminating all the probes. | 12-22-2011 |
20110312817 | MICROFLUIDIC TEST MODULE WITH HUMIDITY SENSOR - A test module for analyzing a sample containing nucleic acid sequences, the test module having a casing configured for hand-held portability, an array of probes housed in the casing, the probes being configured to hybridize with target nucleic acid sequences in the sample to form probe-target hybrids, a humidity sensor for sensing the humidity within the casing, wherein, the humidity sensor generates an output for humidity control within the casing. | 12-22-2011 |
20110312818 | TEST MODULE WITH EXCITATION LIGHT AND LENS FOR SIMULTANEOUS EXCITATION OF OLIGONUCLEOUTIDE PROBES - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, an excitation light for generating the excitation light, and, a lens positioned in the outer casing for directing light from the excitation light to simultaneously illuminate the probes. | 12-22-2011 |
20110312819 | LOC DEVICE FOR DETECTING TARGET NUCLEIC ACID SEQUENCES USING ELECTROCHEMILUMINESCENCE OF A LUMINOPHORE IN THE PRESENCE OF AN ELECTROCHEMICAL COREACTANT - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, an electrochemical coreactant that is present with the luminophore during electrochemiluminescence. | 12-22-2011 |
20110312820 | TEST MODULE WITH EXCITATION LIGHT AND PRISMS FOR SIMULTANEOUS EXCITATION OF OLIGONUCLEOUTIDE PROBES - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, an excitation light for generating the excitation light, and, prisms positioned in the outer casing for redirecting light from the excitation light to simultaneously illuminate the probes. | 12-22-2011 |
20110312821 | MICROFLUIDIC DEVICE WITH WASTE STORAGE - A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer on the supporting substrate for analyzing a fluid, and, a waste reservoir for storage of waste material separated from the fluid. | 12-22-2011 |
20110312822 | GENETIC ANALYSIS LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET NUCLEIC ACID SEQUENCES - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having a supporting substrate, probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, CMOS circuitry between the supporting substrate and the probes for applying a voltage across the electrodes. | 12-22-2011 |
20110312823 | TEST MODULE WITH EXCITATION LIGHT AND MIRRORS FOR SIMULTANEOUS EXCITATION OF OLIGONUCLEOUTIDE PROBES - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, an excitation light for generating the excitation light, and, mirrors positioned in the outer casing for redirecting light from the excitation light to simultaneously illuminate the probes. | 12-22-2011 |
20110312824 | TEST MODULE WITH WASTE STORAGE INCORPORATING POROUS ELEMENT - A test module for analyzing fluid containing target nucleic acid sequences, the test module having a casing for hand-held portability, the casing having a receptacle for receiving the fluid, an array of probes for hybridization with target nucleic acid sequences to form probe-target hybrids, and, a waste accumulator for storage of waste material separated from the fluid, wherein, the waste accumulator has a waste reservoir and a porous element to draw the waste material into the waste reservoir by capillary action. | 12-22-2011 |
20110312825 | LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION WITH ELECTRODE PAIRS HAVING COMPLEMENTARY AND MUTUALLY INTERDIGITATED FINGER FORMATIONS - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, and, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, wherein, the electrodes have an anode and a cathode, each having fingers configured for mutual interdigitation. | 12-22-2011 |
20110312826 | TEST MODULE WITH LASER FOR EXCITATION OF OLIGONUCLEOUTIDE PROBES - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization with a target nucleic acid sequence to form probe-target hybrids, the probe-target hybrids being configured to generate a fluorescence signal in response to an excitation light, a laser positioned in the outer casing for generating the excitation light. | 12-22-2011 |
20110312827 | GENETIC ANALYSIS LOC DEVICE FOR DETECTION OF TARGET SEQUENCES WITH ELECTROCHEMILUMINESCENT LUMINOPHORE AND FUNCTIONAL MOIETY FOR QUENCHING PHOTON EMISSIONS - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having a supporting substrate, probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, an electrochemiluminescent (ECL) luminophore, and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, CMOS circuitry between the supporting substrate and the probes for applying a voltage across the electrodes. | 12-22-2011 |
20110312828 | LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION USING ELECTRODE PAIRS OPTICALLY COUPLED TO PHOTODIODE - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrode pairs in which one electrode in each of the electrode pairs is a working electrode which causes oxidation or reduction of the luminophore to generate an excited species that emits a photon, and, photodiodes corresponding to each of the electrode pairs respectively, wherein, the photodiodes have a planar active surface area for receiving the light from the luminophore, and the working electrode has a surface area optically coupled to the active surface area of the photodiode, the working electrode being configured such that the optically coupled surface area is greater than 50% of the active surface area of the photodiode. | 12-22-2011 |
20110312829 | LOC DEVICE FOR GENETIC ANALYSIS AND ELECTROCHEMILUMINESCENT DETECTION OF TARGET SEQUENCES - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, and, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light. | 12-22-2011 |
20110312830 | GENETIC ANALYSIS LOC DEVICE WITH ELECTROCHEMILUMINESCENT PROBES HAVING A FUNCTIONAL MOIETY FOR QUENCHING PHOTON EMISSIONS CONFIGURED TO CHANGE PROXIMITY TO A LUMINOPHORE UPON FORMING A PROBE-TARGET HYBRID - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, an electrochemiluminescent (ECL) luminophore, and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, wherein during use, the functional moiety changes proximity to the luminophore upon formation of a probe-target hybrid. | 12-22-2011 |
20110312831 | LOC DEVICE FOR DETECTING TARGET NUCLEIC ACID SEQUENCE WITH ELECTROCHEMILUMINESCENT METALORGANIC COMPLEX - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having a supporting substrate, probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) metalorganic complex, and, electrodes for generating an excited state in the metalorganic complex in which the metalorganic complex emits photons of light, and, CMOS circuitry between the supporting substrate and the probes for applying a voltage across the electrodes. | 12-22-2011 |
20110312832 | LOC DEVICE FOR DETECTION OF TARGET SEQUENCES WITH ELECTROCHEMILUMINESCENT PROBES IN HYBRIDIZATION CHAMBERS - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, an array of hybridization chambers, each of the hybridization chambers having a pair of the electrodes respectively and containing a plurality of the probes, the nucleic acid sequence in the probes in each of the hybridization chambers being different to the nucleic acid sequence in at least one other hybridization chamber in the array such that a plurality of target nucleic acid sequences are detectable. | 12-22-2011 |
20110312833 | LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET SEQUENCES USING TRANSPARENT ELECTRODES - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, and, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, wherein, the electrodes are arranged in pairs, one of the electrodes in each electrode pair being a working electrode which causes oxidation or reduction of the luminophore to generate an excited species that emits a photon, the working electrode being transparent to light at the wavelength of the photons emitted by the ECL luminophores. | 12-22-2011 |
20110312834 | LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION USING A RUTHENIUM ORGANIC COMPLEX - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having a supporting substrate, probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and a ruthenium organic complex, electrodes for generating an excited state in the ruthenium organic complex in which the ruthenium organic complex emits photons of light, and, CMOS circuitry for applying a voltage across the electrodes. | 12-22-2011 |
20110312835 | GENETIC ANALYSIS LOC DEVICE WITH ELECTROCHEMILUMINESCENT PROBES AND INTEGRATED PHOTOSENSOR FOR DETECTION OF TARGET SEQUENCES - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, a photosensor for sensing the photons emitted from the ECL luminophore. | 12-22-2011 |
20110312836 | MICROFLUIDIC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET SEQUENCES - A microfluidic device for detecting target nucleic acid sequences in a sample, the microfluidic device having reagent reservoirs for adding reagents to the sample prior to detection of the target nucleic acid sequences, probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, and, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light. | 12-22-2011 |
20110312837 | LOC DEVICE FOR GENETIC ANALYSIS AND ELECTROCHEMILUMINESCENT DETECTION OF TARGET SEQUENCES - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, a photosensor for sensing the photons emitted from the ECL luminophore, wherein, the electrodes are plates of conductive material, the plates having peripheral edges optically coupled to the photosensor. | 12-22-2011 |
20110312838 | MICROFLUIDIC DEVICE WITH ELECTROCHEMILUMINESCENT PROBES AND INTEGRATED PHOTOSENSOR FOR DETECTION OF TARGET MOLECULES - A microfluidic device for detecting target molecules in a fluid, the microfluidic device having reagent reservoirs for adding reagents to the fluid prior to detection of the target molecules, probes for reaction with the target molecules to form probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, a photosensor for sensing the photons emitted from the ECL luminophore. | 12-22-2011 |
20110312839 | GENETIC ANALYSIS LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET SEQUENCES WITH WORKING ELECTRODE IN CONTACT WITH PHOTOSENSOR - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, a photosensor for sensing the photons emitted from the ECL luminophore, wherein, the electrodes have at least one working electrode which causes oxidation or reduction of the luminophore to generate an excited species that emits the photons, the working electrode being positioned immediately adjacent the photosensor. | 12-22-2011 |
20110312840 | MICROFLUIDIC DEVICE WITH SAMPLE INLET, ELECTROCHEMILUMINESCENT PROBES AND INTEGRATED PHOTOSENSOR FOR DETECTION OF TARGET SEQUENCES - A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer with a hybridization section that has an array of electrochemiluminescent (ECL) probes for hybridization with target nucleic acid sequences in a fluid, and electrode pairs for receiving an electrical pulse, the ECL probes being configured to emit a photon of light when hybridized with one of the nucleic acid targets and activated by one of the electrodes, and, a photosensor for detecting photons of light from the ECL probes that have hybridized. | 12-22-2011 |
20120004144 | MICROELECTROPORATION DEVICE FOR GENOMIC SCREENING - We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system. | 01-05-2012 |
20120015851 | METHOD AND DEVICE FOR RAPID, DETECTION OF SPECIFIC REACTIONS USING NON-MAGNETIC AND MAGNETIC NANOPARTICLES - A method and apparatus for conducting specific binding assays with two or more particles is claimed. A non-magnetic nanoparticle capable of binding the target of interest is mixed with a magnetic particle capable of binding a species directed toward binding the same target of interest. Following the application of the mixture to a capillary tube or other container that allows the separation with a magnetic field or other force, the resultant movement of the magnetic or combination of magnetic and non-magnetic assemblies can be read visually or with the aid of an appropriate instrument. | 01-19-2012 |
20120028845 | Sensor for Detecting Biological Agents in Fluid - Systems and apparatus are disclosed for detecting biological agents in fluids. Biosensor apparatus utilizes a sample chamber configured for receiving a fluid sample. Wells accessible from the sample chamber include electrodes deployed for receiving one or more electromagnetic signal from each of the wells. The electrodes are pre-provided with biomaterial deposits selected to attract particular biological agents of interest. An integrated circuit connected with electrodes facilitates electromagnetic signal acquisition and processing of parameters measurable at the well electrodes. | 02-02-2012 |
20120028846 | GAS SENSOR INCORPORATING A POROUS FRAMEWORK - The disclosure provides sensor for gas sensing including CO | 02-02-2012 |
20120028847 | ASSEMBLIES FOR MULTIPLEX ASSAYS - Multiplex binding assay assemblies are disclosed. The assemblies include at least one assay bar that has a top side, a bottom side, and at least one well accessible from the top side of the assay bar, with each well including a side surface, a bottom surface, an open top end. Each well also includes at least one secondary container, with each secondary container including a capillary tube that (i) begins at a location within an interior volume of the well and (ii) ends at a location beneath the bottom side of the assay bar. The assemblies further include a guiding track, which includes a set of two rails, with each rail having its own separate groove. Such grooves are configured to run parallel to each other with a distance between such grooves, with a first groove configured to receive a protruding element (or an end) of a first side of the assay bar, and a second groove configured to receive a protruding element (or an end) of a second side of the assay bar. The set of two separate grooves are configured to allow the assay bar to glide along the grooves from one side to a second side of the guiding track. | 02-02-2012 |
20120028848 | Binding method and apparatus for sorting objects - The present invention relates to a method and apparatus of sorting objects including, providing a sample having wanted objects and unwanted objects; coating a surface of a sample holder with an antibody; placing an eluted sample on the sample holder; binding an antigen in the wanted objects with the antibody on the surface of the sample holder to sort the objects into wanted objects and unwanted objects; separating the wanted objects; and performing PCR-based STR analysis on the wanted objects. In one embodiment, holographic optical trapping is used to further sort the wanted objects. In other embodiments, the wanted objects are sperm and the antibody is a human sperm specific antibody, and the PCR is single cell PCR-based STR analysis. In still other embodiments, the binding is direct or indirect, ligands are used to bind to object-specific organomolecules, and protein A or protein G are used to bind the antibody. | 02-02-2012 |
20120028849 | MICROWAVE ACCELERATED ASSAYS - The present invention provides for increasing fluorescence detection in surface assay systems while increasing kinetics of a bioreaction therein by providing low-power microwaves to irradiate metallic materials within the system in an amount sufficient to increase heat thereby affecting the kinetics of a bioreaction therein. | 02-02-2012 |
20120028850 | MICROFLUIDIC DEVICE FOR SIMULTANEOUSLY CONDUCTING MULTIPLE ANALYSES - Provided is a rotatable microfluidic device for conducting simultaneously two or more assays. The device includes a platform which can be rotated, a first unit which is disposed at one portion of the platform and detects a target material from a sample using surface on which a capture probe selectively binds to the target material is attached, and a second unit which is disposed at another portion of the platform and detects a target material included in the sample by a different reaction from the reaction conducted in the first unit. | 02-02-2012 |
20120028851 | MICROFLUIDIC DEVICE FOR SIMULTANEOUSLY CONDUCTING MULTIPLE ANALYSES - Provided is a rotatable microfluidic device for conducting simultaneously two or more assays. The device includes a platform which can be rotated, a first unit which is disposed at one portion of the platform and detects a target material from a sample using surface on which a capture probe selectively binds to the target material is attached, and a second unit which is disposed at another portion of the platform and detects a target material included in the sample by a different reaction from the reaction conducted in the first unit. | 02-02-2012 |
20120028852 | MICROFLUIDIC DEVICE FOR SIMULTANEOUSLY CONDUCTING MULTIPLE ANALYSES - Provided is a rotatable microfluidic device for conducting simultaneously two or more assays. The device includes a platform which can be rotated, a first unit which is disposed at one portion of the platform and detects a target material from a sample using surface on which a capture probe selectively binds to the target material is attached, and a second unit which is disposed at another portion of the platform and detects a target material included in the sample by a different reaction from the reaction conducted in the first unit. | 02-02-2012 |
20120035084 | DEVICE AND METHOD FOR PARTICLE COMPLEX HANDLING - An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte. | 02-09-2012 |
20120046203 | IMAGING ANALYZER FOR TESTING ANALYTES - The invention provides analyzers that improve tests for detecting specific cellular, viral, and molecular targets in clinical, industrial, or environmental samples. The invention permits efficient and specific selection and sensitive imaging detection of individual microscopic targets at low magnification. Automated embodiments allow efficient walk-away, on-demand, random-access high-throughput testing. The analyzers perform tests without requiring wash steps thus streamlining engineering and lowering costs. Thus, the invention provides analyzers that can deliver rapid, accurate, and quantitative, easy-to-use, and cost-effective tests for analytes. | 02-23-2012 |
20120071363 | METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS - Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis. | 03-22-2012 |
20120071364 | DETECTION OF NUCLEIC ACID SEQUENCE DIFFERENCES USING THE LIGASE DETECTION REACTION WITH ADDRESSABLE ARRAYS - The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. | 03-22-2012 |
20120071365 | Detection Device Having Increased Detection Rate, and Method for Quick Detection of Biological Molecules - A biological molecule detection device that includes a detection array, arranged on a body and having one or more probes for detecting corresponding electrically charged molecules, wherein a time varying electric field generating circuit is provided for generating at least one time varying electric field around the detection array within the detection region. The time varying electric field moves the electrical charged molecules repeatedly back and forth over the probes, thus providing increased opportunities for interaction and speeding the detection process. | 03-22-2012 |
20120088696 | MICRO ELECTROCHEMICAL MULTIPLEX REAL-TIME PCR PLATFORM - A micro electrochemical multiplex Real-Time PCR platform which can be widely used to rapidly amplify, examine, and quantify target nucleotides in real-time, and can be used in sepsis diagnosis, rapid detection of animal/plant viral or bacterial infections, plant disease control, real-time environmental monitoring, food industry contamination prevention, and improvement of agricultural varieties. | 04-12-2012 |
20120108470 | MICROFLUIDIC MAGNETOPHORETIC DEVICE AND METHODS FOR USING THE SAME - A microfluidic device may employ one or more sorting stations for separating target species from other species in a sample. The separation is driven by magnetophoresis. A sorting station generally includes separate buffer and sample streams. A magnetic field gradient applied to the sorting station deflects the flow path of magnetic particles (which selectively label the target species) from a sample stream into a buffer stream. The buffer stream leaving the sorting station is used to detect or further process purified target species labeled with the magnetic particles. | 05-03-2012 |
20120115757 | SELF-SENSING ARRAY OF MICROCANTILEVERS FOR CHEMICAL DETECTION - The invention provides a chemical detection system for detecting at least one target chemical species, including a self-sensed cantilevered probe array having a plurality of self-sensed cantilevered probes, at least one chemical-sensitive coating material applied to at least one cantilevered probe in the cantilevered probe array, and an interface circuit that is coupled to the cantilevered probe array. At least one cantilevered probe in the cantilevered probe array exhibits a shifted cantilevered probe response when the cantilevered probe array is exposed to the target chemical species and the interface circuit actuates the cantilevered probe. A handheld chemical detection system and a method of operation are also disclosed. | 05-10-2012 |
20120129732 | Methods and Apparatus for Measuring Analytes Using Large Scale FET Arrays - Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis. | 05-24-2012 |
20120149603 | SAMPLE ANALYSIS SYSTEM - An integrated sample analysis system is disclosed. The sample analysis system contains (1) a sample preparation/analysis module having sample purification device comprising a monolith that binds specifically to nucleic acids and a sample analysis device comprising a microarray enclosed in a reaction chamber having a hydrophilic interior surface; (2) a temperature control module comprising a thermocycler having a thermally conductive temperature-control bladder; and (3) an imaging device capable of capturing an image of the microarray in the reaction chamber. | 06-14-2012 |
20120157349 | CELL CHIP PACKAGE - There is provided a cell chip package, including: a chamber having an opening formed in a region thereof; a cell chip received in an inside of the chamber and having a biomaterial array formed therein; and a sealing membrane mounted in the opening and made of a gas permeable material. The cell chip package may facilitate experimentation and long-term storage with respect to a cell chip. | 06-21-2012 |
20120157350 | Brownian Microbarcodes for Bioassays - Presented are encoded microparticles, methods of use in biological assays, and flexible, modular instrument systems for conducting a variety of biological assays using the encoded microparticles. The systems include various instrumentation components which are exchangeable and offer a single flexible platform on which a large number of different types of experiments and assays may be conducted including, for example, microarray analysis, encoded microparticle single and multiplex detection, tissue dissection and isolation, and other genetic analysis techniques. | 06-21-2012 |
20120172260 | System and methods for localizing and analyzing samples on a Bio-Sensor chip - Chips that include one or more particle manipulation mechanisms, or force transduction elements, provided at specific locations to manipulate and localize particles proximal the substrate surface. In one embodiment, individually addressable magnetic control mechanisms such as electric coils are provided at specific locations to create a magnetic field to attract magnetic particles, such a magnetic or magnetizable beads, to those specific locations. In another embodiment, electrostatic control mechanisms such as electrodes are provided to attract and manipulate electrically charged micro-particles. A location may include a crater or well formed in the substrate, or it may include an element on the surface of the substrate. In some embodiments, one or more sensors are located proximal specific locations, e.g., specific craters, so as to analyze specific conditions at each location. In other embodiments, multiple locations share one or more sensors. | 07-05-2012 |
20120190588 | Methods for Identifying Agents that Inhibit Cell Migration, Promote Cell Adhesion and Prevent Metastasis - Disclosed are methods for identification of agents that modulate cell attachment, cell migration and cell viability. Cancer and primary cells adhered to a matrix are treated with agent(s) that modulate ActRII signaling and cell adhesion. Agents are tested that modulate cell adhesion, detachment, invasion and viability. Agents that modulate the expression, phosphorylation, function and translocation of ActRII signaling pathway members also can predict agents that modulate cell adhesion, detachment, invasion and viability. The methods have utility in identifying agents that prevent cancer cell metastasis, wound dehiscence, aortic dissection and aid retina attachment and skin replacement and fertility. | 07-26-2012 |
20120190589 | Assay Cartridges and Methods of Using the Same - Assay cartridges are described that have a detection chamber, preferably having integrated electrodes, and other fluidic components which may include sample chambers, waste chambers, conduits, vents, bubble traps, reagent chambers, dry reagent pill zones and the like. In certain embodiments, these cartridges are adapted to receive and analyze a sample collected on an applicator stick. Also described are kits including such cartridges and a cartridge reader configured to analyze an assay conducted using an assay cartridge. | 07-26-2012 |
20120190590 | Assay Plates, Reader Systems and Methods for Luminescence Test Measurements - Luminescence test measurements are conducted using an assay module having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence. | 07-26-2012 |
20120190591 | Assay Plates, Reader Systems and Methods for Luminescence Test Measurements - Luminescence test measurements are conducted using an assay module having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence. | 07-26-2012 |
20120196775 | EMISSION INTENSITY MEASURING DEVICE - An emission intensity measuring device includes a light receiving unit that is disposed opposed to a biochip having a plurality of compartments in which a sample is housed, and includes a plurality of light receiving elements that are arranged, and a determining section that determines a weighting rate of each of the light receiving elements based on a noise characteristic of the light receiving element, acquired in advance. The emission intensity measuring device further includes a multiplying section that multiplies the output of each of the light receiving elements by the weighting rate to calculate a weighted output of each of the light receiving elements, and an adding section that adds the weighted outputs of the light receiving elements opposed to a respective one of the compartments. | 08-02-2012 |
20120252704 | BIOANALYTICAL INSTRUMENTATION USING A LIGHT SOURCE SUBSYSTEM - The invention relates to a light source for irradiating molecules present in a detection volume with one or more selected wavelengths of light and directing the fluorescence, absorbance, transmittance, scattering onto one or more detectors. Molecular interactions with the light allow for the identification and quantitation of participating chemical moieties in reactions utilizing physical or chemical tags, most typically fluorescent and chromophore labels. The invention can also use the light source to separately and simultaneously irradiate a plurality of capillaries or other flow confining structures with one or more selected wavelengths of light and separately and simultaneously detect fluorescence produced within the capillaries or other flow confining structures. In various embodiments, the flow confining structures can allow separation or transportation of molecules and include capillary, micro bore and milli bore flow systems. The capillaries are used to separate molecules that are chemically tagged with appropriate fluorescent or chromophore groups. | 10-04-2012 |
20120264654 | DIAGNOSTIC ELEMENT, AND A DIAGNOSTIC DEVICE COMPRISING A DIAGNOSTIC ELEMENT - The invention relates to a diagnostic element. The diagnostic element comprises an inlet passage, a holding port and an outlet passage. The holding port is capable of encapsulating a diagnostic gel. The invention also relates to a diagnostic device that comprises at least one inlet port, a preparation port, a diagnostic element that comprises an inlet passage, a holding port, an outlet passage and an outlet port. | 10-18-2012 |
20120289434 | SYSTEMS AND METHODS FOR DEVELOPING DIAGNOSTIC TESTS BASED ON BIOMARKER INFORMATION FROM LEGACY CLINICAL SAMPLE SETS - Disclosed are systems and methods for developing diagnostic tests (e.g., detection, screening, monitoring, and prognostic tests) based on biomarker information from legacy clinical sample sets, for which only small sample volumes (e.g., about 0.05 to about 1.0 mL or less per sample) are typically available. For example, biomarkers (e.g., about 10, 50, 100, 150, 200, 300, or more) may be detected in the clinical samples through the use of single molecule detection and each biomarker may be detected in an assay that includes about 1 μL or less of a legacy clinical sample. | 11-15-2012 |
20130017979 | SENSOR SUBSTRATE AND ARRAY SUBSTRATE USING THE SAME - A sensor substrate includes a base and fibrous projections on an upper surface of the base. The fibrous projections have fixed ends fixed to the upper surface of the base and free ends opposite to the fixed ends, respectively. The fibrous projections provide a fixed end-side region including the fixed ends and a free end-side region including the free ends. The total surface area at which the fibrous projections in the fixed end-side region is larger than that in the free end-side region. | 01-17-2013 |
20130065797 | AUTOMATED SAMPLE HANDLING INSTRUMENTATION, SYSTEMS, PROCESSES, AND METHODS - The present invention provides a processing station for automatically processing a biological sample, a system for automated real-time inventory control of consumables within a biological sample handling or assay instrument, a high throughput random access automated instrument for processing biological samples, an automated instrument for processing or analysis of a sample, and processes for automated mucoid detection and elimination. Methods of using the disclosed instruments, mucoid detection processes, and systems to process and/or analyze samples are also disclosed. | 03-14-2013 |
20130079253 | MICROARRAY-BASED SAMPLE ANALYSIS SYSTEM - A microarray-based sample analysis (MBSA) system includes a cartridge holder adapted to receive a replaceable cartridge that is configured to receive a detachable, replaceable sample analysis unit containing one or more reaction chambers for sample analysis; a fluid control subsystem that controls fluid flow; and an optical subsystem configured to capture an image of the microarray. | 03-28-2013 |
20130079254 | Microfluidic dialysis device - A microfluidic dialysis device that has a first channel for receiving a biological sample, a second channel, and a series of adjacent channels extending between the first channel and the second channel. Each of the adjacent channels pin a meniscus of the sample that arrests capillary flow between the first channel and the second channel. A bypass channel between the first channel and the second channel, the bypass channel joining the second channel upstream of the adjacent channels and is configured for uninterrupted capillary driven flow from the first channel to the second channel. Flow from the bypass channel reaches the meniscus pinned at each of the adjacent channels after the meniscus has formed to sequentially remove each of the menisci and allow flow from the first channel to the second channel via the adjacent channels as well as the bypass channel. | 03-28-2013 |
20130096035 | SELF-SUSTAINED FLUIDIC DROPLET CASSETTE AND SYSTEM FOR BIOCHEMICAL ASSAYS - A fluidic cartridge for biochemical assays includes a cartridge body defining a first droplet region and a second droplet region with a droplet restraining barrier therebetween. The droplet restraining barrier has a gap between the first and the second droplet regions. The fluidic cartridge also includes a first droplet dispensed in the first droplet region. The first droplet includes a plurality of magnetic particles dispersed therein. The fluidic cartridge also includes a second droplet disposed in the second droplet region. The plurality of magnetic particles are sufficiently small to be drawn through the gap between the first and second droplet regions when compelled by an applied magnetic field, and the first droplet is restrained by the restraining barrier while the plurality of magnetic particles are drawn through the gap. A biochemical assay system includes a stage adapted to receive a fluidic cartridge, and a magnetic control assembly that includes a magnet. The magnet of the magnetic control assembly is movable to direct motion of magnetic particles contained within the fluidic cartridge. | 04-18-2013 |
20130137607 | POINT OF CARE IMMUNIZATION TESTING SYSTEM - A point of care immunization system based upon microfluidics and micro-titration technologies to rapidly test a patient in order to ascertain an immunization profile so that vaccinations can be administered to address identified gaps. A point of care system comprised of uniquely shaped and color distinguishing sample and test cartridges, with said test cartridges configured to meet healthcare requirements of national governing bodies. A point of care system including an easy access vaccine storage device with indicators to provide data on viability of stored vaccines. | 05-30-2013 |
20130143775 | METHOD FOR DETECTING BIOMOLECULES ELECTRICALLY AND BIOCHIP THEREFOR - The present invention relates to a method for detecting the presence and/or the reaction of a biomolecule by monitoring changes of electrical property accurately according to the biological, biochemical or chemical reaction of the biomolecule, and a biochip provided for this purpose. | 06-06-2013 |
20130184187 | SYSTEMS AND METHODS FOR BIOMARKER ANALYSIS - Systems and methods of biomarker analysis. In at least one embodiment of a system for determining the therapeutic potential of a therapeutic compound the present disclosure, the system comprises a detection platform, a computer database capable of receiving a plurality of binding characteristics, a processor operably coupled to the computer database and the detection platform. In at least one embodiment, the processor has and executes a software program operational to determine a binding characteristic of the detection agent and a stabilized diagnostic agent in each of the plurality of detection sites, compare the binding characteristic among each of the plurality of detection sites, wherein the comparison of binding characteristics is capable of determining the stabilizing agent with the greatest effect on the binding characteristic between the detection agent and the diagnostic agent, generate a binding record using the compared binding characteristics, and deliver the binding record to a recipient. | 07-18-2013 |
20130184188 | Integrated Test Device for Optical Detection of Microarrays - This invention relates generally to devices and methods for performing optical and electrochemical assays and, more particularly, to testing devices having an optically readable microspot array and/or an electrochemical detector and to methods of performing microspot arrays and electrochemical assays using such devices. The present invention is particularly useful for performing immunoassays and/or electrochemical assays at the point-of-care. | 07-18-2013 |
20130190213 | Microfluidic Methods, Devices, and Systems for Fluid Handling - In one aspect of the invention, systems, methods, and devices are provided for handling liquid. In some embodiments, such systems, methods, and devices are used to process reagent biochemical reactions. | 07-25-2013 |
20130225453 | BIOMATERIAL DETECTING DEVICE - Provided is a biomaterial detecting device for confirming or detecting a biomaterial reaction, and more particularly to a biomaterial detecting device, which is formed in a stick type to thereby be immersed in a tube containing a biomaterial solution to be tested; has an upper portion with a cap structure to thereby induce reaction with a biomaterial, and thus facilitate confirmation and detection of the biomaterial; and is formed in a cap structure to thereby prevent evaporation of a sample and infiltration of an external material at the time of a biomaterial reaction in the tube, and thus improve reliability in analysis. | 08-29-2013 |
20130244909 | METHODS AND APPARATUS FOR CLASSIFICATION AND QUANTIFICATION OF MULTIFUNCTIONAL OBJECTS - A method may include accessing data regarding a number of events, where the events were detected by a particle detection apparatus, and identifying a number of groups in the events. Each of the groups includes two or more events, and each event includes one or more of a time stamp, a time duration, a fluorescence intensity, a scatter measurement, a radio frequency signal, a magnetic signal, a neutron scattering measurement, a light scattering measurement, an electron scattering measurement, an audio signal, an acoustic signal, a mechanical signal, an electrical resistance, a thermal property, and a width of fluorescence signal. The method may include identifying a subset of the groups as a number of objects detected by the particle detection apparatus, where each object is identified based at least in part upon one or more quantities, where each quantity is identified by or derived from the respective two or more events. | 09-19-2013 |
20130267442 | Systems and Methods for Developing Diagnostic Tests Based on Biomarker Information from Legacy Clinical Sample Sets - Disclosed are systems and methods for developing diagnostic tests (e.g., detection, screening, monitoring, and prognostic tests) based on biomarker information from legacy clinical sample sets, for which only small sample volumes (e.g., about 0.05 to about 1.0 mL or less per sample) are typically available. For example, biomarkers (e.g., about 10, 50, 100, 150, 200, 300, or more) may be detected in the clinical samples through the use of single molecule detection and each biomarker may be detected in an assay that includes about 1 μL or less of a legacy clinical sample. | 10-10-2013 |
20130267443 | ncRNA AND USES THEREOF - The present invention relates to compositions and methods for cancer diagnosis, research and therapy, including but not limited to, cancer markers. In particular, the present invention relates to ncRNAs as diagnostic markers and clinical targets for prostate, lung, breast and pancreatic cancer. | 10-10-2013 |
20130303409 | AUTOMATICALLY CONTROLLING A PLURALITY OF DEVICES OF A SEPARATION AND DETECTION PROCESS FOR QUANTITATIVE SAMPLE ANALYSIS - A control system ( | 11-14-2013 |
20130345097 | Sample Block Apparatus and Method for Maintaining a Microcard on a Sample Block - A thermal cycling device for thermally cycling samples of biological material contained in a microcard having a top and bottom surface. The thermal cycling device can include a sample block having an upper surface configured for engaging the bottom surface of a microcard, a vacuum device, and a temperature control system operatively connected with the sample block. The upper surface of the sample block may include a plurality of channels, the channels defining spaces between the sample block and the bottom surface of a microcard that may be positioned thereon. The vacuum device may be in fluid communication with the sample block for drawing gas out of the spaces defined by the channels in the sample block. The vacuum device may be configured for substantially maintaining a vacuum between the sample block and microcard so that a retention force is imparted on the microcard to urge the microcard toward the sample block. Methods of maintaining a microcard on a sample block of a thermal cycling device are also provided. | 12-26-2013 |
20140005078 | OPTICAL SYSTEM FOR CHEMICAL AND/OR BIOCHEMICAL REACTIONS | 01-02-2014 |
20140018266 | MANUFACTURING AND PROCESSING POLYMER ARRAYS - The invention provides methods to process multiple microarrays by providing a microarray plate and processing plates. In an embodiment of the invention, methods for assembling microarray plates by using wafers are described for high throughput microarray processing. | 01-16-2014 |
20140038855 | High Pressure Parallel Fixed Bed Reactor and Method - Apparatus and methods provide rapid analysis of members of a combinatorial library. The apparatus includes a plurality of reactor vessels for containing individual library members, a fluid handling system that apportions a test fluid about equally between each of the vessels, and a housing for enclosing the reactor vessels. The housing defines a pressure chamber configured to sustain a pressure substantially above atmospheric pressure. This allows for simultaneous screening of library members at high pressure by providing a small pressure differential on reactor components. The apparatus is used for screening library members based on their ability to catalyze the conversion of fluid reactants. | 02-06-2014 |
20140045730 | INTEGRATED PLASMONIC SENSING DEVICE AND APPARATUS - An integrated plasmonic sensing device is monolithically integrated and provides marker-free detection (eliminating the need to use fluorescent or absorbing markers) and in-situ monitoring of conditions at each detection region. The integrated plasmonic sensing device includes a plasmonic backplane disposed on a monolithically integrated image sensor. One or more plasmonic scattering regions and one or more plasmonic via regions laterally offset from the plasmonic scattering regions are provided in the plasmonic sensing device. Guided plasmonic modes mediate power transfer through the plasmonic backplane to one or more underlying image sensor pixels. | 02-13-2014 |
20140087973 | INTEGRATED CARRIER FOR MICROFLUIDIC DEVICE - An injection molding method of fabricating a carrier for holding a microfluidic device can form all of the desired features of such a carrier, including wells, channels and ports having smaller dimensions and greater density than previously achieved, while reducing or avoiding fracturing and the need for drilling the substrate to form certain features, in particular the ports. The carrier includes a substrate with a plurality of wells, each well defining a volume of between 0.1 μl and 100 μl; a plurality of channels within the substrate wherein each well is in fluid communication with at least one of the plurality of channels; a plurality of ports within the carrier substrate wherein each port is for coupling with regions in the carrier substrate adapted to receive fluids or pressure; and a receiving portion for receiving a microfluidic device and placing the microfluidic device in fluid communication with the plurality of wells. The carrier has a polymeric composition and/or an array of structural features achieved via the injection molding fabrication technique that enhance its performance and compatibility with existing instrumentation. | 03-27-2014 |
20140100141 | Compartementalized integrated biochips - An integrated microfluidic biochip is provided that includes a microfluidic device, where the microfluidic device includes hollow structures, where at least one the hollow structure includes an output at a bottom surface of the microfluidic device, and a sensor plate, where the sensor plate comprises a plurality of independent surface sensors, where the microfluidic device is sealably attachable to the sensor plate, where the hollow structure output abuts the surface sensor when the microfluidic device is attached to the sensor plate. | 04-10-2014 |
20140113840 | ANALYTE DETECTION SYSTEM WITH CLEANING PHASE AND RENEWABLE LIQUID SENSING MATERIAL AND METHODS THEREFORE - Methods and systems for detecting analytes using a sensor element having a cleaning cycle and renewable liquid material with an affinity for the analytes are described. An analyte detection system may include a controller in communication with a sensor element and a liquid dispensing assembly. The liquid dispensing assembly, such as an inkjet dispensing device, may deposit the liquid material on or adjacent to the sensor element. The controller may be operative to monitor at least one property associated with the liquid material deposited on the sensor element. In addition, the controller may be configured to generate at least one control signal based on the at least one property. The liquid dispensing assembly may be configured to deposit the at least one liquid material on the sensor element based on the at least one control signal. | 04-24-2014 |
20140148365 | Assays - A method for assaying a sample for each of multiple analytes is described. The method includes contacting an array of spaced-apart test zones with a liquid sample (e.g., whole blood). The test zones are disposed within a channel of a microfluidic device. The channel is defined by at least one flexible wall and a second wall which may or may not be flexible. Each test zone comprising a probe compound specific for a respective target analyte. The microfluidic device is compressed to reduce the thickness of the channel, which is the distance between the inner surfaces of the walls within the channel. The presence of each analyte is determined by optically detecting an interaction at each of multiple test zones for which the distance between the inner surfaces at the corresponding location is reduced. The interaction at each test zone is indicative of the presence in the sample of a target analyte. | 05-29-2014 |
20140155298 | MOLECULAR PROFILING OF TUMORS - Provided herein are methods and systems of molecular profiling of diseases, such as cancer. In some embodiments, the molecular profiling can be used to identify treatments for a disease, such as treatments that were not initially identified as a treatment for the disease or not expected to be a treatment for a particular disease. | 06-05-2014 |
20140162908 | BIO-CHIP - There is provided a bio-chip, including a fixing plate having a plurality of guide grooves formed in one surface thereof, a first substrate having a plurality of support plates inserted into the guide grooves, and a plurality of pillars protruded from one surface of the respective support plates, and having a biomaterial disposed thereon. | 06-12-2014 |
20140171343 | BIOLOGICAL DETECTING CHIP - A biological detecting chip comprising an optical fiber, at least one gas filter, an upper cap and a substrate is disclosed. The optical fiber has at least one detecting area disposed on an outer surface. The upper cap has at least two guiding channels passed through the upper cap, at least one discharge channel with two ends connecting to an upper portion of distinct guiding channels, an inlet and an outlet, wherein the gas filter is attached to an upside of the discharge channel to separate the discharge channel and an outside of the upper cap. The substrate has a test area and a plurality of directing channels, wherein the directing channel connects to the inlet and the guiding channel, connects to the guiding channel and the test area, and connects to the test area and the outlet. | 06-19-2014 |
20140171344 | Sample Plate Systems and Methods - A sample plate comprising a sample well is disclosed. The sample well can comprise one or more bead retaining chambers. Also provided herein is a method of using the sample plate and kits comprising the sample plate. | 06-19-2014 |
20140179566 | Thermal Cycling Apparatus and Method - A system for holding at least one of sample and reagent for analysis. The system includes a pair of parallel covers, at least one of which is light transmissive, of which pair a light transmissive cover forms a top, and of which pair the other forms a bottom. A frame is disposed between the covers to define, in relation to the covers, an interior volume. The frame and the covers are associated with one another to form a case, the case being substantially tight to liquids. A microfluidic array is disposed in the interior volume. The array includes a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of through-holes running through the thickness between the surfaces, the through-holes containing at least one of sample and reagent. | 06-26-2014 |
20140187448 | Radio Frequency Identifiers for Use in Biological Science - Provided herein are biological research methods, kits, and products that utilize radio frequency identifier technology. | 07-03-2014 |
20140200167 | FUNCTIONALLY INTEGRATED DEVICE FOR MULTIPLEX GENETIC IDENTIFICATION - A biochip for multiplex genetic identification is disclosed. An biochip for separating and detecting a plurality of DNA fragments includes a set of inputs and chambers for receiving a sample matrix of genetic material and reagents needed to conduct a polymerase chain reaction amplification of the genetic material. The biochip also includes a plurality of separation and detection chambers that physically separate different DNA fragments that have been marked with labels that emit similar colors, thereby enabling the independent detection of the different DNA fragments. | 07-17-2014 |
20140213486 | COMPENSATOR FOR MULTIPLE SURFACE IMAGING - A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention. | 07-31-2014 |
20140213487 | Systems and Methods for Biological Analysis - A biological analysis system is provided. The system comprises an interchangeable assembly configured to accommodate any one of a plurality of sample holders, each respective sample holder configured to receive a plurality of samples. The system also includes a control system configured to cycle the plurality of samples through a series of temperatures. The system further includes an optical system configured to detect fluorescent signals emitted from the plurality of samples. The optical system, in particular, can comprise a single field lens, an excitation source, an optical sensor, and a plurality of filter components. The excitation source can be one or more light emitting diodes. The field lens can be a bi-convex lens. | 07-31-2014 |
20140235511 | MOLECULAR PROFILING OF TUMORS - Provided herein are methods and systems of molecular profiling of diseases, such as cancer. In some embodiments, the molecular profiling can be used to identify treatments for a disease, such as treatments that were not initially identified as a treatment for the disease or not expected to be a treatment for a particular disease. | 08-21-2014 |
20140287965 | METHOD FOR DETERMINING THE PRESENCE OF DISEASE - The invention provides a method for determining presence of a disease, comprising steps of; measuring the levels of expression of transcription products of genes in a biological sample obtained from a subject suspected of having a target disease, wherein the genes comprise at least one gene belonging to each of at least two disease-determining gene families related to the target disease; obtaining values representing deviations by standardizing the levels of the expression based on the levels of expression of transcription products of the corresponding genes in a plurality of healthy subjects; obtaining the average of values representing deviations with respect to the gene belonging to each of the disease-determining gene families; and determining whether or not the subject has the target disease by using the average; as well as a computer program product for determining presence of a disease. | 09-25-2014 |
20140287966 | MICROFLUIDIC RECONFIGURATION DEVICE FOR MULTI-PLEXED SAMPLE ANALYSIS - A reconfigurable microfluidics multiplexing device for biological and chemical sample analysis which comprises: a cartridge comprising one or more sample fluid tracks (both horizontal and vertical) in a single plane, an array comprising one or more electronically programmable valves capable of being configured to alter fluid steering in the fluid tracks; a means for the array to interface with the fluid tracks; a means to control fluid flow in the fluid tracks; and a user interface to direct control of valves via control logic within the device; wherein, by the interface, valve positions are controllable and alterable. | 09-25-2014 |
20140296112 | OPTICAL TESTING SYSTEM - The present application provides a system ( | 10-02-2014 |
20140303043 | REVERSIBLE REACTION SENSORS AND ASSEMBLIES - A reversible reaction sensor provides for detecting medical, biologic or explosive airborne compounds. The sensor may be formed in semiconductor material and is activated by radiation from sources to provide sensing of particular airborne compounds optically detectable by detectors, and reversed by other radiation from a source (or removal of activating radiation) to take away such airborne compounds from the sensor. The reversible reaction sensor device has a sensing material of one or more photo-chromic or photo-biologic compounds for a specific compound(s) or analytic(s) having receptor sites which bound molecularly to specific compound(s) or analytic(s) when present, in which sensing relies only on the response time to saturation for the sensing material as measured by an optical property of the sensing material when exposed to radiation. The sensing material is self cleaning by one or more of light of a specific wavelength, radio waves of a specific frequency, absence of said light illuminating said material, absence or presence of a magnetic field, which causes the receptor sites to close and the bound specific analytic are released and swept away by flow of air. The sensor may be in hermetically sealed and non-hermetic assemblies. | 10-09-2014 |
20140336083 | SYSTEM FOR PORTABLE AND EASY-TO-USE DETECTION OF ANALYTES WITH MOBILE COMPUTING DEVICE - This system takes in raw cellular material collected using a provided swab, blood collection device, urine collection device, or other sample collection device and transforms that biological material into a digital result, identifying the presence, absence and/or quantity of nucleic acids, proteins, and/or other molecules of interest. | 11-13-2014 |
20140364342 | MICROARRAY PROCESSING APPARATUS, WELL PLATE FOR MICROARRAY PROCESSING APPARATUS, MICROARRAY HOLDER, AND MICROARRAY WASHING METHOD - Provided is a microarray processing apparatus which is capable of sufficiently washing a microarray. The microarray processing apparatus ( | 12-11-2014 |
20140371111 | SYSTEM AND METHOD FOR DETERMINING INDIVIDUALIZED MEDICAL INTERVENTION FOR A DISEASE STATE - A system and method for determining individualized medical intervention for a particular disease state, and especially for cancers, that includes the molecular profiling of a biological sample from the patient, determining whether any molecular findings including one or more genes, one or more gene expressed proteins, one or more molecular mechanisms, and/or combinations of such exhibit a change in expression compared to a reference, and identifying a non-specific disease therapy or agent capable of interacting with the genes, gene expressed proteins, molecular mechanisms, or combinations of such molecular findings that exhibited a change in expression. | 12-18-2014 |
20140371112 | Method and Apparatus for Holding Cells - The present invention pertains to an apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises a mechanism for determining the state of the cells. The determining mechanism is in communication with the incubating mechanism. The present invention pertains to a method for holding cells. The method comprises the steps of incubating the cells in a dynamically controlled environment which is maintained in a desired condition and in which the cells can be examined while the environment is dynamically controlled and maintained in the desired condition. Additionally, there is the step of determining the state of the cells. | 12-18-2014 |
20140378351 | SYSTEM AND METHOD FOR PROCESSING GENOTYPE INFORMATION RELATING TO TREATMENT WITH PAIN MEDICATION - There are systems and methods for performing an assay to generate genotype information about a subject associated with a chronic pain condition. There are also systems and method for generating and utilizing prognostic information associated with treating the patient with a pain medication based on the genotype information. The genotype information includes data relating to specific SNP alleles in the patient's genotype. | 12-25-2014 |
20140378352 | MICROFLUIDIC PARTICLE-ANALYSIS SYSTEMS - The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system. Accordingly, these combinations may allow particles to be sorted, cultured, mixed, treated, and/or assayed, among others, as single particles, mixed groups of particles, arrays of particles, heterogeneous particle sets, and/or homogeneous particle sets, among others, in series and/or in parallel. In addition, these combinations may enable microfluidic systems to be reused. Furthermore, these combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays. | 12-25-2014 |
20150011437 | Multi-unit plate for immunoblot analysis - This invention provides for multi-unit plates that comprise a layer of membrane, preferably nitrocellulose or PVDF membrane, on the surface of individual unit of the multi-unit plate. These multi-unit plates are particularly well suited for high throughput immunoblot analysis including Zestern analysis. | 01-08-2015 |
20150011438 | DETECTION CHIP AND METHOD FOR USING THE SAME - A detection chip is provided. The detection chip includes a substrate, an active reagent, a hydrophilic droplet and a lipophilic substance. The substrate includes a first containing slot, wherein the first containing slot includes a first space and a second space adjacent to each other. The active reagent is disposed in the first space of the first containing slot. The hydrophilic droplet is disposed in the second space of the first containing slot. The lipophilic substance is disposed in the first containing slot, wherein the lipophilic substance is immiscible to the active reagent and the hydrophilic droplet, and separates the active reagent from the hydrophilic droplet. | 01-08-2015 |
20150031586 | ASSAY DEVICE AND METHOD - There is provided an assay device comprising a lid and a base, said base comprising, a sample addition zone, a reaction zone and an absorbing zone, said components being in fluid connection and being part of a fluid passage leading from the sample addition zone to the absorbing zone, wherein: (a) a sample addition well is integrated in the lid, (b) the absorbing zone consists of an area on an non-porous substrate, having substantially perpendicular projections, said projections defining a volume, which together with the volume of the fluid passage defines the sample volume subjected to the assay, and (c) at least one filter is between the sample addition well and the sample addition zone. There is further provided methods for handling samples. | 01-29-2015 |
20150031587 | BIOCHIP AND TARGET DNA QUANTITATIVE METHOD - A biochip used for quantitative analysis of a target DNA contained in a sample. The biochip includes a type I chamber that includes a primer designed to bind to the target DNA, an internal standard DNA of a first amount that has a sequence different from a sequence of the target DNA, and is amplifiable with the primer, and a fluorescent probe that is designed to bind to a part of a PCR product of the target DNA and to a part of a PCR product of the internal standard DNA. The fluorescent probe fluoresces differently for the PCR product of the target DNA and the PCR product of the internal standard DNA. The biochip also includes a type II chamber that includes the internal standard DNA of a second amount, the primer, and the fluorescent probe. The first and second amounts are different. | 01-29-2015 |
20150038378 | BIOCOMPATIBLE GRAPHENE SENSOR - A graphene biosensor is formed on an electrically insulating substrate with a single-layer graphene sheet arranged between two metallic electrodes. The graphene sheet is in electrical contact with the metallic electrodes. The graphene sheet has perforations creating edges in the graphene sheet. The perforations may be holes on a micrometer scale or in a nanometer scale. The biosensor can be configured as an ISFET. The graphene sheet may comprise affinity probes immobilized on the edges for attaching specific molecules to the graphene sheet. Several graphene sheets may be arranged in a microarray with different affinity probes on different graphene sheets. The sensor may also be arranged on the distal end of a catheter for in situ measurements in a body vessel. | 02-05-2015 |
20150045259 | HIGH-THROUGHPUT PLATFORM FOR IN-VIVO SUB-CELLULAR SCREENS ON VERTEBRATE LARVAE - High throughput system for in vivo screens on vertebrate larvae. The system includes a source of vertebrate larvae in a liquid medium and loading tube means for aspirating a larva. A detector assembly is provided to differentiate passage of a larva from bubbles and/or debris. An imaging means is provided for both confocal imaging and wide-field fluorescence imaging of the larva. A laser is provided for optical manipulation of the larva. | 02-12-2015 |
20150057193 | ENDOTHELIAL-MONOCYTE ACTIVATING POLYPEPTIDE II (EMAP-II), A BIOMARKER FOR USE IN DIAGNOSIS AND TREATMENT OF BRAIN INJURY - A diagnostic tool and method of diagnosing brain injury and brain injury type (traumatic vs. ischemic) by detecting the level of expression of endothelial monocyte-activating polypeptide II (EMAP II) and comparing to a control. An increase of EMAP II indicates the presence of traumatic brain injury and a decrease of EMAP II indicates the presence of ischemic brain injury. Detection of EMAPII can be done in brain tissue, biofluids such as cerebrospinal fluid or blood (including plasma and serum) | 02-26-2015 |
20150057194 | Integrated Analytical System and Method - An analytical assembly within a unified device structure for integration into an analytical system. The analytical assembly is scalable and includes a plurality of analytical devices, each of which includes a reaction cell, an optical sensor, and at least one optical element positioned in optical communication with both the reaction cell and the sensor and which delivers optical signals from the cell to the sensor. Additional elements are optionally integrated into the analytical assembly. Methods for forming and operating the analytical system are also disclosed. | 02-26-2015 |
20150065397 | ASSAY ASSEMBLY - An assay assembly includes an assay plate. The assay plate includes at least one hydrophobic area and a plurality of hydrophilic areas defined by the hydrophobic area, wherein the hydrophilic areas are defined as a plurality of assay areas. Due to the specialized structure of the assay plate, droplets around the assay areas are drawn to the assay area by pushing force of the hydrophobic area and the pulling force of the assay area. In addition, the shaker is coupled to the assay plate and configured for shaking the assay plate thereby droplets around the assay areas are further drawn to the assay area by shaking of the shaker. The present invention may improve the error-tolerance rate of the assay plate and be used for high-throughput screening. | 03-05-2015 |
20150087559 | PDMS Membrane-Confined Nucleic Acid and Antibody/Antigen-Functionalized Microlength Tube Capture Elements, and Systems Employing Them - A microfluidic device comprising a microfluidic channel network sealed on one side by a membrane sheet, the sheet having PDMS defining at least the surface sealing the channel, the membrane sheet on its opposite side sealing one side of a pneumatic channel, the pneumatic channel arranged to enable pneumatic deflection of a deflectable portion of the membrane sheet into contact with an opposed surface to control flow in a channel of the network, the membrane sheet confining in a channel of the network at least one micro-particle, micro-length tube or glass nano reactor, functionalized with a capture agent, that has been inserted into that channel. A microfluidic device having a microfluidic channel containing at least two micro-particles, micro-length tubes or glass nano reactors, one functionalized with nucleic acid and another with antibody or antigen. A microfluidic device having a microfluidic channel containing at least one micro-length tube or glass nano reactor functionalized to capture nucleic acid, the device constructed to enable recovery of the nucleic acid captured by the device. | 03-26-2015 |
20150111790 | TESTING DEVICE - The invention is a device for testing an individual for the presence of a entity. The entity can be a pathogen, toxin or drug. The device is adapted to collect from the individual the biological sample to be tested. The test device comprises noble metal nanoparticles (i.e. gold, silver or copper) to detect the entity. Test results are read from a window in the device. The current visual limit using aggregated noble metal nanoparticles is a clump having a diameter in a range of about 50 nm to about 75 nm. One or more ligands specific for the entity are attached to two or more metal nanoparticles. Each individual nanoparticle has a diameter in a range of about 15 nm to about 35 nm. Ligands attached to the nanoparticles and specific for the entity can be for example antibodies, antibody fragments, oligonucleotides, and aptamers. Different ligands specific for different sites on the entity may be used in order to increase the aggregation potential of the nanoparticles in the presence of the target. The testing is conducted within the device after sample collection. The results of assay are available promptly. Conditions that can be tested include for example viral infections, bacterial infections, exposure to toxins, and drug use. Detection of Human Immunodeficiency Virus (HIV) is described by example. | 04-23-2015 |
20150119298 | ANALYSIS ENGINE AND DATABASE FOR MANIPULATING PARAMETERS FOR FLUIDIC SYSTEMS ON A CHIP - A system for performing one or more microfluidic processes includes an integrated fluidic device comprising a plurality of well regions and a plurality of control valves and a workflow manager. The system also includes a transfer robot adapted to transfer the integrated fluidic device between a plurality of stations in response to a series of instructions from the workflow manager and a first station comprising a dispensing robot adapted to dispense at least one of a plurality of sample solutions and at least one of a plurality of reagents into the integrated fluidic device. The system further includes a second station comprising a fluidic controller unit and a third station comprising an inspection station. | 04-30-2015 |
20150133345 | FLUORESCENCE DETECTOR FOR MICROFLUIDIC DIAGNOSTIC SYSTEM - The present technology provides for a fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently. | 05-14-2015 |
20150148265 | SYSTEMS AND DEVICES FOR PROFILING MICROBIOTA OF SKIN - Devices and systems for sampling and profiling microbiota of skin are described which include a replaceable microbe sampling unit including a substrate and a location information storage component, the substrate including a microbe-capture region configured to capture at least one type of microbe from one or more regions of a skin surface of an individual, and the location information storage component configured to store information associated with the location of said one or more regions of the skin surface of the individual. | 05-28-2015 |
20150292017 | Systems For Identifying Cosmetic Agents For Skin Care Compositions - A system for identifying connections between a cosmetic agent and at least one gene associated with a skin aging condition. The system includes a computer readable medium having stored thereon a plurality of instances and a skin aging gene expression signature. Each instance comprises an ordered list of identifiers representing a plurality of differentially expressed genes generated as a result of contact between the cosmetic agent and a human fibroblast cell or a human keratinocyte cell. The computing device includes computer-readable instructions for accessing the plurality of instances and the skin aging gene expression signature stored on the computer readable medium, comparing each of the instances to the skin aging gene expression signature, and assigning a connectivity score to each of the instances based on the comparison. | 10-15-2015 |
20150301026 | DEVICES, SYSTEMS AND METHODS FOR HIGH-THROUGHPUT ELECTROPHYSIOLOGY - Electrophysiology culture plates are provided and are formed from a transparent micro-electrode array (MEA) plate. The MEA plate comprises a substrate, a first layer and a first insulating layer. The substrate has a plurality of vias extending from an upper to a lower surface, each via being in electrical contact with each of a plurality of contact pads disposed on the lower surface. The first layer is disposed on the upper surface of the substrate and has a plurality of MEA arrays in in electrical communication with at least a first routing layer. Each MEA array comprises a plurality of reference electrodes and a plurality of microelectrodes and the first routing layer is in electrical communication with a select number of the plurality of vias. A first insulating layer is disposed on the first layer. The MEA plate is joined to a biologic culture plate having a plurality of culture wells such that each culture well defines an interior cavity having a bottom surface that is at least partially transparent and in positioned in registration with a select optical port. The MEA plate is coupled to the biologic culture well plate such that each MEA array is operatively coupled to one culture well wherein each microelectrode and each reference electrode are in electrical communication with the interior cavity through the bottom surface of the culture well. | 10-22-2015 |
20150308957 | Method and Apparatus for Melting Curve Analysis of Nucleic Acids in Microarray Format - A method and apparatus for performing melting curve analyses of nucleic acids on a microarray is described. The present method includes varying the temperature of a fluid on a microarray to dissociate and remove target DNA, scanning the mircoarray for fluorescence, collecting the target DNA removed from the microarray, and reusing the collected target DNA and the microarray. The apparatus of the present disclosure includes a microarray stage, a light source and detector, and a temperature controller, wherein the temperature controller is configured to adjust the temperature of a fluid within a sample chamber on the microarray such that the temperature of the fluid is varied during the analysis such that target DNA is dissociated from the microarray, and wherein the light source is directed to the microarray and the resulting fluorescence is perceived by the detector. | 10-29-2015 |
20150323555 | INTEGRATED MULTIPLEX TARGET ANALYSIS - This invention provides biochip cartridges and instrument devices for the detection and/or analysis of target analytes from patient samples. | 11-12-2015 |
20150329822 | DIFFERENTIATED CELL POPULATION OF ENDOTHELIAL CELLS DERIVED FROM HUMAN PLURIPOTENT STEM CELLS, COMPOSITION, SYSTEM, KIT AND USES THEREOF - The present disclosure relates to a differentiated cell population of endothelial cells derived from human pluripotent stem cells. The present invention also relates to a composition, a system and a kit comprising those cells and uses thereof. The present disclosure also described the combination of arterial ECs derived from human pluripotent stem cells with a microfluidic system to create a vascular kit for high-throughput drug screening and/or toxicology analysis. This technology may find particular use for the identification of drugs that may have a fetal cytotoxic effect. | 11-19-2015 |
20150330879 | METHOD AND APPARATUS FOR BIOMOLECULE ANALYSIS - Disclosed are devices or apparatus and methods for membrane-, bead- or cell-based biomolecule analysis, where a detectable signal can be measured after affinity binding and/or enzymatic reaction occurs on the membranes, beads or cells. The apparatus or device can rapidly separate the beads or cells from the liquid solution without using centrifugation, vacuum, or magnetic force. The apparatus or device includes a sample well for receiving a sample containing cells or beads, as well as other aqueous solutions, a porous filter membrane at the bottom of the well which is capable of retaining the cells or beads on its upper surface by size exclusion, and an absorbing plug in touch with the filter membrane for removing the liquid solution. Multiple devices can be arranged in a multi-well array in a plate so that signals from multiple assays can be directly analyzed in a plate reader or an imaging instrument. | 11-19-2015 |
20150355165 | Microfluidic grid-based design for high throughput assays - Microfluidic chips have been developed to perform a range of high-throughput biochemical and cell-based assays over recent years. We have formulated an innovative design for a microfluidic chip that resembles a microtiter plate by placing the test chambers (each test chamber contains cells) and top-loading drug inlets (one per test chamber) in a grid according to the ANSI/SBS standards, yet offers the miniaturization and fluid handling advantages of microfluidics. This ensures that our chip design is compatible with fluid handling and imaging equipment already in use for drug screening. We have determined a range of topologies that allow placement of various elements of this microfluidic network within the grid alignment constraints. We also developed a resistance equalization methodology to reduce variability across assays run in different chambers of the microfluidic chip. Additionally, it offers orders of magnitude miniaturization over multiwell plates, and potentially more reliable fluid handling. | 12-10-2015 |
20150369721 | DEVICE FOR THE STUDY OF LIVING CELLS - A device for performing a cell study. The device comprises a plate having a plurality of wells, each configured for containing aqueous solution and having a well bottom with a plurality of picowells and a plurality of biosensors each configured for measuring at least one cell characteristic while being in contact with the aqueous solution in a respective the well. The position of each the biosensor in a respective the well is limited by at least one pin. | 12-24-2015 |
20150377875 | Fluidic Medical Devices and Uses Thereof - This invention is in the field of medical devices. Specifically, the present invention provides fluidic systems having a plurality of reaction sites surrounded by optical barriers to reduce the amount of optical cross-talk between signals detected from various reaction sites. The invention also provides a method of manufacturing fluidic systems and methods of using the systems. | 12-31-2015 |
20150377914 | MODULAR POINT-OF-CARE DEVICES, SYSTEMS, AND USES THEREOF - The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications. | 12-31-2015 |
20160001250 | TEMPERATURE REGULATION OF MEASUREMENT ARRAYS - A system for regulating a temperature of a measurement array is disclosed. The system includes a measurement array including a plurality of sensors, wherein the plurality of sensors are integrated onto an integrated circuit die. The system includes a thermal sensor integrated onto the integrated circuit die, wherein the thermal sensor senses a temperature associated with the plurality of sensors. The system further includes a heat pump coupled to the integrated circuit die, wherein the heat pump is controlled by a feedback control circuit including the thermal sensor. | 01-07-2016 |
20160003841 | INTEGRATED HIGH THROUGHPUT SYSTEM FOR THE ANALYSIS OF BIOMOLECULES - Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules. | 01-07-2016 |
20160018310 | White Blood Cell Analysis System and Method - Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WB Cs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis. | 01-21-2016 |
20160018311 | Basophil Analysis System and Method - Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements. | 01-21-2016 |
20160018312 | Nucleated Red Blood Cell Analysis System and Method - Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs. | 01-21-2016 |
20160025667 | APPARATUS FOR THE DETECTION OF LIQUIDS OR SUBSTANCES FROM LIQUIDS - An apparatus for detecting liquids or substances from liquids in spatially separate reaction zones using a plug-in module or a chip card for rapid immunological tests, for example, with the help of a reading device. The liquids or substances from liquids are detected by a sensor array and on which at least one diaphragm is arranged. Individual sensors are spatially separated from each other on a plane by means of walls. The diaphragm is filled with liquid that is to be analyzed. Sealed reaction chambers are created when pressure is applied to the diaphragm. Pores in the diaphragm are completely closed in the zone of the walls while the pores are merely reduced in size and liquid can continue to be exchanged in zones directly above the sensors. No liquid can be exchanged between adjacent reaction chambers as long as pressure is applied to and compresses the diaphragm. | 01-28-2016 |
20160041096 | WAVEGUIDE GRATING STRUCTURE AND OPTICAL MEASUREMENT ARRANGEMENT - The present invention describes (bio)chemo-functional waveguide grating structures consisting of at least one (bio)chemo-functional waveguide grating structure unit or at least one (bio)chemo-functional sensor location with beam guidance permitting light beam separation, as well as detection methods for parallel analysis which are marking-free or based on marking. | 02-11-2016 |
20160041156 | Integrated Device for Measuring Multiple Analytes - Integrated Multiplexed Point-of-Care device for measuring levels of target analytes from biological samples for determination of disease state. Specifically, a platform with a self contained fluidic cartridge comprising direct sample introduction, onboard sample processing, and multiplexed analyte detection is described. | 02-11-2016 |
20160047770 | CELL-BASED BIOSENSOR ARRAY AND ASSOCIATED METHODS FOR MANUFACTURING THE SAME - A cell-based biosensor array includes a base plate having a plurality of substantially transparent areas. The cell-based biosensor array also includes a flexible substrate coupled to the base plate and having disposed thereon a plurality of electrode sets, a plurality of terminal contacts, and a plurality of conductive traces. Each electrode set is disposed proximate a respective one of the substantially transparent areas, and each electrode set includes at least one electrode configured to detect an electric signal. Each terminal contact is associated with a respective one of the at least one electrode and disposed proximate a perimeter of the flexible substrate. Each conductive trace is electrically coupling a respective at least one electrode to the corresponding terminal contact. A first portion of flexible substrate including the electrode sets is disposed on a first surface of the base plate. A second portion of the flexible substrate including the terminal contacts is disposed on a second surface of the base plate. | 02-18-2016 |
20160051980 | DESIGN OF PAPER SENSOR - The present disclosure proposes a design of a biomedical paper sensor which can determine the concentration of biological materials in fluids such as blood, urine, and saliva. The sensor contains a plurality of axially radiating test zones, each test zone separated from other test zones by wax ink barriers formed by a process that produces thin walls. Each test zone can contain a unique test reagent, or a unique concentration of a test reagent, and can also be identified by printed text. The region of the device outside of the test zone area is printed with a uniform reference color. Benefits of the invention include increased accuracy in the measurement of the concentration of biological materials due to the larger test zone area. Benefits also include the integration of the reference or calibration color which simplifies the calibration needed for quantification of the concentration. | 02-25-2016 |
20160054312 | CHEMICALLY DIFFERENTIATED SENSOR ARRAY - A chemically differentiated sensor array system includes a plurality of environmentally-gated transistors and an environmental gate, wherein the environmental gate includes a liquid solution and each environmentally-gated transistor includes a drain, a source, and a Carbon-based substrate channel, the drain electrically couples to a first location on the substrate channel, the source electrically couples to a second location on the substrate channel separated by a gap from the first location on the substrate channel, the environmental gate covers and contacts the substrate channel, a first insulating layer covers and separates the drain from the environmental gate, and a second insulating layer covers and separates the source from the environmental gate. | 02-25-2016 |
20160054397 | Correlated double sampling for noise reduction in magnetoresistive sensors and sensor arrays - Correlated double sampling (CDS) for magnetoresistive (MR) sensors is provided. Here the MR sensor output is sampled at two closely spaced times. The first sample is MR signal+baseline+noise and is sampled when the modulated magnetic field is non-zero. The second sample is baseline+noise only because it is sampled when the modulated magnetic field is zero. The difference between the first and second samples will have significantly reduced low frequency noise and baseline cancellation. Modulation of the electrical bias provided to the MR sensor can be used to provide a baseline signal for temperature compensation. In a second aspect, we provide MR sensor arrays having input and output multiplexing and demultiplexing for row and column line selection, in combination with a per-sensor switch to prevent noise accumulation and bandwidth reduction from idle MR sensors. | 02-25-2016 |
20160061823 | TARGET SUBSTANCE CAPTURING DEVICE - A target substance capturing device includes a reflection surface on which a plurality of non-flat portions is arrayed, the reflection surface capturing a target substance, and reflecting irradiated light. The plurality of non-flat portions are arranged in an array, the array includes a plurality of unit arrays in which the plurality of non-flat portions are arranged such that each one center of the non-flat portions superposes a position of a vertex in an M-time symmetrical figure, and the plurality of unit arrays is arranged such that each one center of gravity of the M-time symmetrical figure superposes a position of an intersection of an N-time symmetrical lattice pattern, where M is an integer of two or more, and N is an integer of two or more and different from M. | 03-03-2016 |
20160074861 | Portable Analytic Device and Methods of Use Thereof - A portable analytic apparatus is provided that is capillary-driven and capable of detecting analytes within a sample and providing an unambiguous positive/negative response. Embodiments provide for a hydrophobic detection reagent within a hydrophilic porous media, where a hydrophobic detection reagent changes to hydrophilic products in a presence/absence of a target analyte. This change from hydrophobic to hydrophilic provides a means to achieve a desired result of generating an unambiguous qualitative and/or quantitative positive/negative readout by forcing samples to wick at differential rates through hydrophilic regions towards a detection region. Suitable hydrophobic detection reagents are also provided. | 03-17-2016 |
20160091427 | METHOD FOR MANUFACTURING BIOCHIP HAVING IMPROVED FLUORESCENT SIGNAL SENSING PROPERTIES AND BIOCHIP MANUFACTURED BY THE SAME - A method for manufacturing a biochip having improved fluorescent signal sensing properties, and a biochip manufactured by the manufacturing method. A filter layer is provided between a bio-layer and a light sensor layer so as to remove noise generated by stray light during a bio-reaction process. Thereby, the sensitivity of the light sensor layer can be enhanced. | 03-31-2016 |
20160109406 | PULSE-FIELD MULTIPLEX CAPILLARY ELECTROPHORESIS SYSTEM - The invention is a multiplex, pulsed-field capillary electrophoresis instrument with the ability to analyze DNA fragments with sizes greater than 50,000 bp, greater than 100,000 bp and even granter than 150,000 base pairs. The parallel capillary electrophoresis system allows for the simultaneous analysis of at least 12 samples while applying a pulse or varying electric field for separation. | 04-21-2016 |
20160109474 | HIGHLY AUTOMATED CAPILLARY ELECTROPHORESIS SYSTEM - The invention is an improved multiplex capillary electrophoresis instrument or module with at least four and preferably six user-accessible vertically stacked drawers. An x-z stage moves samples from the user accessible drawers to the capillary array for analysis. An additional mechanical stage moves the array from side-to-side. The x-z stage, coupled with the additional array stage allows the system to sample all wells of a 384 well plate with a 96-capillary array. A computer program allows users to add capillary electrophoresis jobs to a queue corresponding to the analysis of rows or plates of samples without stopping or interrupting runs in progress. | 04-21-2016 |
20160115528 | MEASURING DEVICE AND SYSTEM FOR PERFORMING MELTING CURVE ANALYSIS OF A DNA MICROARRAY AND UTILIZATION OF A FLUORESCENCE DETECTOR ARRAY FOR ANALYSIS - What is shown is a measuring device for simultaneously analyzing melting curves of DNA samples in DNA microarrays with the aid of a fluorescence detector array. Embodiments show monitoring of melting curves of DNA microarrays (dynamic fluorescence measurement) by means of silicon photomultipliers (SiPM) or other photodetectors such as, e.g., PIN diodes (positive intrinsic negative diodes), avalanche photodiodes (APD), or photomultiplier tubes (PMT). The DNA microarray is applied, along with a thin-film heating element, to a substrate made of plastic or glass or is integrated in a microfluidic channel. | 04-28-2016 |
20160116495 | ROTATING SAMPLE POSITIONING APPARATUS - A positioning system for a sample analysis device is disclosed. The positioning system comprises (1) a carousel comprising a platform and a sample loading tray mounted on the platform, and (2) a stage comprising a positioning system for positioning the carousel under the optical path of an imaging system. The sample loading tray is configured for holding a cartridge comprising one or more lateral flow cells (LFCs). | 04-28-2016 |
20160130640 | CARTRIDGE FOR PERFORMING ASSAYS IN A CLOSED SAMPLE PREPARATION AND REACTION SYSTEM - In one embodiment, a multiplex fluid processing cartridge includes a sample well, a deformable fluid chamber, a mixing well with a mixer disposed therein, a lysis chamber including a lysis mixer, an electrowetting grid for microdroplet manipulation, and electrosensor arrays configured to detect analytes of interest. An instrument for processing the cartridge is configured to receive the cartridge and to selectively apply thermal energy, magnetic force, and electrical connections to one or more discrete locations on the cartridge and is further configured to compress the deformable chamber(s) in a specified sequence. | 05-12-2016 |
20160131636 | SYSTEM AND METHOD FOR HIGH-THROUGHPUT ASSESSMENT OF CELLULAR CARDIOTOXICITY, DRUG SCREENING, AND CARDIOGENIC FACTORS VIA ONLINE PHYSIOLOGICAL MEASUREMENTS - An integrated system for detecting safety and/or efficacy issues related to potential drug compounds by combining tissue maturation, imaging and electrophysiology measurements, at high throughput, with high signal to noise ratio. Testing includes cardiotoxicity screening, drug screening, and screening for cardiogenic factors via on-line physiological measurements. | 05-12-2016 |
20160145676 | SYSTEMS FOR BIOAGENT IDENTIFICATION - The present invention provides systems and methods for analysis of samples, particularly biological and environmental sample to detect biomolecules of interest contained therein. A variety of system components are described herein, including, but not limited to, components for sample handling, mixing of materials, sample processing, transfer of materials, and analysis of materials. The invention further provides mechanisms for combining and integrating the different components and for housing, moving, and storing system components or the system as a whole. The systems may include any one or more or all of these components. The system finds particular use when employed for analysis of nucleic acid molecule using mass spectrometry, however, the invention is not limited such specific uses. | 05-26-2016 |
20160146718 | BIOPOLYMER SEPARATION USING NANOSTRUCTURED ARRAYS - A technique relates sorting biopolymers. The biopolymers are introduced into a nanopillar array, and the biopolymers include a first population and a second population. The nanopillar array includes nanopillars arranged to have a gap separating one from another. The biopolymers are sorted through the nanopillar array by transporting the first population of the biopolymers less than a predetermined bumping size according to a fluid flow direction and by transporting the second population of the biopolymers at least the predetermined bumping size according to a bumped direction different from the fluid flow direction. The nanopillar array is configured to employ the gap with a gap size less than 300 nanometers in order to sort the biopolymers. | 05-26-2016 |
20160146802 | POROUS MEMBRANES WITH A POLYMER GRAFTING, METHODS AND USES THEREOF - A device comprising a modified porous membrane is provided. The modified porous membrane comprises a polymer coating grafted to a porous membrane. The device is used for analyte detection from a biological sample using an immunoassay. The device comprises a sample application zone at one end of the device for applying a biological sample comprising a target analyte; and a detection zone present at another end of the device, downstream of the sample application zone for detecting the target analyte, wherein the detection zone comprises one or more first biomolecules immobilized on a modified porous membrane having a structure of Formula (I). | 05-26-2016 |
20160153978 | AUTOMATED CHEMICAL ANALYSIS APPARATUS WITH INTEGRATED MICROFLUIDIC MICROCHIP | 06-02-2016 |
20160161465 | MULTIWELL MICROELECTRODE ARRAY WITH OPTICAL STIMULATION - An electro-optical stimulation and recording system is disclosed, including a substrate and a plurality of wells coupled to the substrate. The system also includes at least one electrode set disposed proximate a respective one of the plurality of wells, wherein the electrode set comprises at least one electrode configured to collect an electric signal associated with at least a portion of the respective well. The system also includes a light-emitting element set corresponding to a respective one of the wells and configured to deliver optical stimulation to at least a portion of the respective well. | 06-09-2016 |
20160178504 | FLOW CYTOMETRY FOR HIGH THROUGHPUT SCREENING | 06-23-2016 |
20160187328 | DEVICES, SYSTEMS, AND METHODS FOR MULTIPLEX ALLERGEN TESTING - Multiplex devices, systems, and associated methods for assaying a biological sample for an allergen-binding molecule are disclosed and described. | 06-30-2016 |
20160202209 | ELECTRIC FIELD DIRECTED LOADING OF MICROWELL ARRAY | 07-14-2016 |
20160375436 | DETECTION DEVICE - A detection device includes a carrying unit, at least a reaction unit, a wire transporting unit, and a guiding unit. The carrying unit includes at least a hole, and the reaction unit is disposed on one side of the hole. The reaction unit includes at least a reaction area, which includes a hydrophilic material containing a detection reagent. The wire transporting unit is disposed on the other side of the hole and includes a sampling end and a transporting end. The guiding unit is in contact with the reaction unit and the transporting end. The guiding unit collects a test sample through the transporting end and provides at least a part of the test sample to react with the detection reagent. The detection device adopts the wire transporting unit to replace the pipette for collecting the test sample without directly contacting the detection area with the sample source. | 12-29-2016 |
20160375438 | MICROFLUIDIC CARTRIDGE FOR MOLECULAR DIAGNOSIS, DOCKING STATION USING A MICROFLUIDIC CARTRIDGE, AND PROCESS FOR ANALYZING A BIOLOGICAL SAMPLE - A microfluidic cartridge for detecting one nucleic acid of a sample, including a plurality of functional volumes split into functional areas and a fluidic network of microchannels. At least three functional areas are fluidly connected to a central distribution hub of fluids by one or more hub-connected microchannels, the central distribution hub being capable of pumping and injecting fluids from a first functional area to a second functional area by passing through the central distribution hub; and at least three valves of hub-connected microchannels are arranged so that the at least three valves are adapted to be actuated mechanically by a single external cam-driven actuator. A docking station using such a microfluidic cartridge and a process for analyzing a biological sample involving a docking station and a microfluidic cartridge are also described. | 12-29-2016 |
20180023051 | DIFFERENTIATED CELL POPULATION OF ENDOTHELIAL CELLS DERIVED FROM HUMAN PLURIPOTENT STEM CELLS, COMPOSITION, SYSTEM, KIT AND USES THEREOF | 01-25-2018 |
20220137036 | CONTROLS FOR IMPLEMENTING MULTIPLEX ANALYSIS METHODS - The present invention relates to controls which may be used to secure the results of multiplex analysis methods. The present invention thus relates to solid supports comprising one or several controls and their use in multiplex analysis methods to detect several analytes potentially present in a sample. | 05-05-2022 |