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Peptides or polypeptides, or derivatives thereof

Subclass of:

506 - Combinatorial chemistry technology: method, library, apparatus

506013000 - LIBRARY, PER SE (E.G., ARRAY, MIXTURE, IN SILICO, ETC.)

506015000 - Library containing only organic compounds

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DocumentTitleDate
20130029879Methods and Systems for Cell State Quantification - Systems, methods, libraries, kits, and computer software tools are provided for designing and producing engineered cells. Such engineered cells can be used for cell state quantification, such as genome, transcriptome and/or proteome quantification. In one aspect, an engineered cell having a plurality of artificially designed oligonucleotides introduced into the genome of the cell is provided. The oligonucleotides are each located in proximity of a gene of interest encoding a protein of interest, and are different from one another. The oligonucleotides can each encode a unique peptide tag for each protein of interest, wherein each peptide tag has a unique quantitatively measurable value such as mass-to-charge ratio which can be quantified by a mass spectrometer. The engineered cell is capable of expressing a plurality of proteins of interest each fused to its corresponding unique peptide tag, wherein each peptide tag is capable of being released therefrom.01-31-2013
20090143248Biologically active C-terminal arginine-containing peptides - The present invention concerns the separation, identification and characterization of active peptide fragments from peptones.06-04-2009
20130079250Compound Arrays for Sample Profiling - The invention provides arrays of compound for use in profiling samples. The arrays include compounds bind to components of the samples at relatively low affinities. The avidity of compounds binding to components of the samples can be increased by forming arrays such that multivalent components of the samples (e.g., antibodies or cells) can bind to more than one molecule of a compound at the same time. When a sample is applied to an array under such conditions, the compounds of the array bind to component(s) of the sample with significantly different avidities generating a profile characteristic of the sample.03-28-2013
20130079249GLYCOSYLATION ASSAY, GLYCOANALYSIS ARRAY AND AN ASSAY SYSTEM - An improved glycosylation assay, glycoanalysis array and an assay system for performing glycosylation assays glycoanalysis array.03-28-2013
20130040861NOVEL METHODS OF CONSTRUCTING LIBRARIES COMPRISING DISPLAYED AND/OR EXPRESSED MEMBERS OF A DIVERSE FAMILY OF PEPTIDES, POLYPEPTIDES OR PROTEINS AND THE NOVEL LIBRARIES - Methods useful in constructing libraries that collectively display and/or express members of diverse families of peptides, polypeptides or proteins and the libraries produced using those methods are disclose. Methods of screening those libraries and the peptides, polypeptides or proteins identified by such screens are also disclosed.02-14-2013
20120165230BIOLOGICALLY PRODUCED CYCLIC AFFINITY TAGS - Described are novel ways of introducing an affinity tag into a protein of interest. Provided is an enzymatic method for providing a proteinaceous substance comprising a polypeptide of interest and a cyclic affinity tag, comprising: (a) providing at least one precursor proteinaceous substance, the precursor comprising the protein of interest and at least one motif of the general formula X1-Tag-X2, wherein X1 and X2 represent amino acids whose side chains can be linked enzymatically by a covalent bond; Tag is a short amino acid sequence capable of binding to a binding partner of the tag when cyclized; (b) contacting the precursor with at least one enzyme capable of forming a covalent bond between X1 and X2, thereby introducing an intramolecular ring structure comprising the Tag sequence; and (c) isolating the resulting cyclized proteinaceous substance. Also provided are proteinaceous substances obtainable thereby and the use thereof, for instance, for preparing a peptide library.06-28-2012
20090124517Microarray having a chemical library of compounds - A microarray and a process for making the microarray having a library of chemical compounds are disclosed herein. Each member of the library is attached at a known location. The microarray has a plurality of addressable electrodes and a porous reaction layer attached to each electrode. The porous reaction layer has reactive hydroxyl groups. Michael acceptors are attached to the porous reaction layer of selected electrodes. The Michael acceptors are attached at the hydroxyl groups using electrochemically-generated base that is generated by the selected electrodes and confined to the selected electrodes with the use of excess activated ester. At least one of a plurality of chemical compounds from a library of compounds is attached to the olefin of the plurality of Michael acceptors. Attachment occurs only at the selected electrodes having Michael acceptors with unreacted olefin. Each member of the library of compounds is attached to the known locations.05-14-2009
20130090263FLUORESCENT PROTEINS, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM - The invention is directed to polypeptides having a fluorescent activity, e.g., an auto-fluorescent activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as noninvasive fluorescent markers in living cells and intact organs and animals. The polypeptides of the invention can be used as, e.g., in vivo markers/tracers of gene expression and protein localization, activity indicators, fluorescent resonance energy transfer (FRET) markers, cell lineage markers/tracers, reporters of gene expression and as markers/tracers in protein-protein interactions.04-11-2013
20120238478PROTEIN MARKERS FOR THE DIAGNOSIS AND PROGNOSIS OF OVARIAN AND BREAST CANCER - Plasma samples of ovarian and breast cancer patients were used to search for markers of cancer, using two-dimensional gel electrophoresis and MALDI TOF mass spectrometry. Truncated forms of cytosolic serine hydroxymethyl transferase (cSHMT), T-box transcription factor 3 (Tbx3) and utrophin were aberrantly expressed in samples from cancer patients, as compared to samples from noncancer cases. Aberrant expression of proteins was validated by immunoblotting of plasma samples with specific antibodies to cSHMT, Tbx3 and utrophin. A cohort of 79 breast and 39 ovarian cancer patients, and 31 individuals who were either healthy or had noncancerous conditions was studied. We observed increased expression of truncated cSHMT, Tbx3 and utrophin in plasma samples obtained from patients at early stages of disease. The results indicate that cSHMT, Tbx3, utrophin and truncated forms thereof can be used as components of multiparameter monitoring of ovarian and breast cancer.09-20-2012
20120238477METHODS FOR SYNTHESIS OF AN OLIGOPEPTIDE MICROARRAY - An oligopeptide microarray and methods for the synthesis thereof are presented. Further presented is a microarray on a solid support comprising at least about 10,000 oligopeptide features per cm09-20-2012
20120238476ALZHEIMER'S DISEASE DIAGNOSTIC PANELS AND METHODS FOR THEIR USE - Novel compositions, methods, assays and kits directed to a diagnostic panel for Alzheimer's disease are provided. In one embodiment, the diagnostic panel includes one or more proteins associated with Alzheimer's disease.09-20-2012
20120142558DIAGNOSTIC LUNG CANCER PANEL AND METHODS FOR ITS USE - Disclosed herein are novel diagnostic lung cancer panels and the use of these panels to diagnose, predict, and characterize lung cancer and to monitor or predict treatment efficacy.06-07-2012
20090181863SCD Fingerprints - This invention relates to methods of testing, diagnosing, monitoring, prognostically stratifying and classifying disease, disorders and other medical conditions and physiological states through the detection and analysis of soluble CD antigens in a body fluid sample.07-16-2009
20110301065IDENTIFYING ANTIGEN CLUSTERS FOR MONITORING A GLOBAL STATE OF AN IMMUNE SYSTEM - Method, system and an article of manufacture for clustering and thereby identifying predefined antigens reactive with undetermined immunoglobulins of sera derived from patient subjects in need of diagnosis of disease or monitoring of treatment.12-08-2011
20090023602BINDING POLYPEPTIDES WITH RESTRICTED DIVERSITY SEQUENCES - The invention provides variant CDRs comprising highly restricted amino acid sequence diversity. These polypeptides provide a flexible and simple source of sequence diversity that can be used as a source for identifying novel antigen binding polypeptides. The invention also provides these polypeptides as fusion polypeptides to heterologous polypeptides such as at least a portion of phage or viral coat proteins, tags and linkers. Libraries comprising a plurality of these polypeptides are also provided. In addition, methods of and compositions for generating and using these polypeptides and libraries are provided.01-22-2009
20100222236MULTIPLE ANTIGENIC PEPTIDE ASSAY FOR DETECTION OF HIV OR SIV TYPE RETROVIRUSES - A method for detecting at least one antibody directed against at least one primate immunodeficiency virus in a biological sample that includes contacting a biological sample with (i) at least one detection multiple antigenic peptide comprising a portion of an immunodominant region of a transmembrane protein of a primate immunodeficiency virus and (ii) at least one differentiation multiple antigenic peptide comprising a portion of a V3-loop of an envelope protein of a primate immunodeficiency virus. Also disclosed is an enzyme immunoassay that includes a first substrate to which are bound at least one of the detection multiple antigenic peptides and a second substrate to which are bound at least one of the differentiation multiple antigenic peptides.09-02-2010
20110166043BIOMARKER DETECTION-2 - Provided are methods, compositions and articles of manufacture for detecting biomarkers indicative of exposure of a mammal to organophosphate compounds. The organophosphate compound includes pesticides, their metabolites and highly reactive organophosphoryl compounds. In one aspect of the invention, the biomarker results from interaction of the organophosphate compound with a polypeptide such as a serine hydrolase that includes acetylcholiestersae. The interaction of a biomarker so derived with a optical sensor comprising a receptor bound to a biopolymer results in an optical readout that reports the presence of the biomarker. In one aspect of the invention the receptor that is bound to a biopolymer, such as a poly-di-acetylene polymer, is a antibody that selectively recognizes the biomarker.07-07-2011
20110294702TEMPLATE FIXED BETA-HAIRPIN LOOP MIMETICS AND THEIR USE IN PHAGE DISPLAY - Template-fixed β-hairpin mimetics and libraries including a plurality of these mimetics are provided. The template-fixed β-hairpin mimetics are of the following general formula:12-01-2011
20090221449Presentation of Recognition Motifs by a Multivalent Matrix Grafted Onto a Solid Support - The invention relates to a method for preparing a grafted homodetic cyclopeptide forming a frame defining two surfaces, one surface being known as the upper surface and the other surface being known as the lower surface, both surfaces being grafted, characterized in the a linear peptide is synthesized, said synthesis is being carried out on modified amino acids or not, some of which include orthogonal protector groups, intramolecular cyclization of the protected linear peptide thus obtained is performed, all or part of the orthogonal protector groups are substituted by a protected precursor, and at least one molecule of therapeutic interest is grafted on one and/or the other surface of the frame by means of an oxime link.09-03-2009
20110130309ASSAY SYSTEM TO IDENTIFY THERAPEUTIC AGENTS - The invention is an assay designed to identify agents that modulate apoptosis. The invention provides an assay system and methods for screening for inhibitors of the Bcl-2 family of proteins. In various aspects the invention provides an assay system containing a liposome reagent and an immobilized BH3 domain peptide. In further aspects the invention provides an assay system containing mitochondria, an immobilized BH3 domain peptide and a mitochondrial binding agent, e.g. an anti-VDAC anti-body.06-02-2011
20090258796MASSIVELY PARALLEL SYNTHESIS OF BIOPOLYMERIC ARRAYS - Methods for fabricating dense arrays of polymeric molecules in a highly multiplexed manner are provided using semiconductor-processing-derived lithographic methods. Advantageously, the methods are adaptable to the synthesis of a variety of polymeric compounds. For example, arrays of branched peptides and polymers joined by peptide bonds may be fabricated in a highly multiplexed manner.10-15-2009
20110172126LIBRARIES OF PEPTIDE CONJUGATES AND METHODS FOR MAKING THEM - The invention relates to peptide conjugates including at least one turn inducer wherein the turn inducer comprises a 5-7 membered saturated or unsaturated nitrogen containing heterocyclic ring and methods of making the peptides. Libraries of these peptides, methods of making the libraries are also described and methods of screening the libraries for therapeutic activity are also described.07-14-2011
20130217600METHODS OF DETERMINING EFFICACY OF TREATMENTS OF DISEASES OF THE BOWEL - Novel methods of determining efficacy of a treatment of inflammatory diseases of the bowel in mammals are provided. The methods are of use in screening and determining the efficacy of treatments of inflammatory diseases of the bowel, and for determining the efficacy response of individual sufferers of inflammatory diseases of the bowel to a given regime. Kits for carrying out the method are also provided.08-22-2013
20120065106Methods and Compositions for Enhanced Protein Expression and Purification - Methods for enhancing expression levels, secretion, and purification of heterologous fusion proteins in a host cell are disclosed.03-15-2012
20090005267IMMUNOASSAY FOR CROSS-REACTING SUBSTANCES - The present disclosure provides an immunoassay involving a multiplex of antibodies that recognize the same analyte but that have a different cross-reactivity to structurally similar compounds. Data obtained from the immunoassay involving observed analyte concentrations is input into an algorithm to determine the true concentration of the analyte in a sample.01-01-2009
20110224101Tumor associated proteome and peptidome analyses for multiclass cancer discrimination - Methods are provided for classification of cancer based on analysis of serologic biomarkers.09-15-2011
20110143963MOLECULAR AFFINITY CLAMP TECHNOLOGY AND USES THEREOF - The invention provides a molecular affinity clamp. The architecture of the affinity clamp is modular with two biorecognition modules, each capable of binding a target motif. The first biorecognition module has a recognition domain that possesses inherent or natural specificity for the target motif. The second biorecognition module also has a recognition domain that binds the motif. The two biorecognition modules are tethered together either directly, e.g., via a peptide bond between the two modules, or indirectly, e.g., via a linker moiety or linker.06-16-2011
20090082223METHOD AND KIT FOR THE DETERMINATION OF CELLULAR ACTIVATION PROFILES - A method for obtaining an activation profile of a biological sample by disposing onto a solid support in a pre-determined spatial arrangement a subset of capture molecules able to interact with one or more activated transcription factor(s) present in the biological sample, contacting the biological sample upon the solid support under conditions allowing their interaction, monitoring signals resulting from their interaction, and providing a cellular activation profile from the detected signals.03-26-2009
20090203550METHODS OF MAKING GLYCOMOLECULES WITH ENHANCED ACTIVITIES AND USES THEREOF - Methods to rapidly produce and identify polysaccharides, and other sugar structures, having enhanced activities, have been developed. The methods include producing a molecule, e.g., a therapeutic molecule, which includes a first, non-saccharide moiety (e.g., a protein, polypeptide, peptide, amino acid or lipid) and a second, polysaccharide, moiety. The method includes: determining the chemical composition and structure of all or a portion of the second moiety, modifying the structure of the second moiety to provide a modified second moiety, and evaluating or screening the molecule having the modified second moiety, e.g., for a biological activity or other chemical or physical property. In some embodiments, the step of determining the chemical structure and composition of the second moiety includes a comparison of one or more properties of the second moiety with a database, e.g., a database which correlates such one or more properties with structure or function of a polysaccharide.08-13-2009
20120077714Mass Spectrometry Based Particle Separation - Certain embodiments provide a method of sample analysis that comprises: a) labeling a particle using a specific binding reagent that is cleavably linked to an elemental tag; b) flowing the labeled particle through a flow cell of a mass cytometer; c) cleaving the elemental tag from the labeled particle; d) performing elemental analysis of the cleaved elemental tag without destroying the particle, to produce data; e) matching data for the particle with the particle; and f) collecting the particle. Also provided is a specific binding reagent that is cleavably linked to an elemental tag, and a mass cytometer adapted to perform the method.03-29-2012
20080318808MASSIVELY PARALLEL SYNTHESIS OF BIOPOLYMERIC ARRAYS - Methods for fabricating dense arrays of polymeric molecules in a highly multiplexed manner are provided using semiconductor-processing-derived lithographic methods. Advantageously, the methods are adaptable to the synthesis of a variety of polymeric compounds. For example, arrays of branched peptides and polymers joined by peptide bonds may be fabricated in a highly multiplexed manner.12-25-2008
20090221448FUSION PROTEIN MICROARRAYS AND METHODS OF USE - The present invention provides a microarray having one or more fusion proteins non-covalently attached to a solid support. Non-covalent attachment is achieved by designing a fusion protein having a polyanionic domain attached to a subject protein, and attaching the fusion protein to a solid support having a polycationic coating.09-03-2009
20090280999Epitope-Captured Antibody Display - Reagents and methods for detecting target proteins in a sample are provided. The reagents include a replicable genetic package, a protein displayed on an exterior surface of the package that is expressed from a heterologous nucleic acid borne by the package, and one or more antibodies complexed with the expressed protein and which have an open binding site for a target protein. Thus, a segment of the nucleic acid encodes for an epitope that is shared by the expressed polypeptide and the target protein. The reagents can be utilized individually or as part of a library or an array to bind target proteins within protein samples to form one or more complexes. By determining the sequence of the segment of the heterologous nucleic acid of a package within a complex, one can identify the target protein since the segment encodes for an epitope that is shared by the expressed and target proteins.11-12-2009
20100190662METHODS AND MATERIALS FOR DETECTION, DIAGNOSIS AND MANAGEMENT OF OVARIAN CANCER - The subject invention concerns methods using a panel of proteins to detect, diagnose, and monitor therapy during treatment of ovarian cancer in a female patient. The proteins were identified using proteomics analyses of plasma samples obtained preoperatively from ovarian cancer patients versus those of healthy control women. Such a panel has utility for the diagnosis of ovarian cancer, screening for ovarian cancer and possibly therapeutic monitoring.07-29-2010
20100167957METHODS FOR PRODUCING ACTIVE SCFV ANTIBODIES AND LIBRARIES THEREFOR - The present disclosure describes scFv antibody libraries, antibodies isolated from the libraries, and methods of producing and using the same.07-01-2010
20100184626ARRAYS OF BIOLOGICAL MEMBRANES AND METHODS AND USE THEREOF - The present invention overcomes the problems and disadvantages associated with prior art arrays by providing an array comprising a plurality of biological membrane microspots associated with a surface of a substrate that can be produced, used and stored, not in an aqueous environment, but in an environment exposed to air under ambient or controlled humidities. Preferably, the biological membrane microspots comprise a membrane bound protein. Most preferably, the membrane bound protein is a G-protein coupled receptor, an ion channel, a receptor serine/threonine kinase or a receptor tyrosine kinase.07-22-2010
20110237462IMMOBILIZATION SUBSTRATE AND METHOD FOR PRODUCING THE SAME - An antibody-fragment-immobilizing substrate includes a substrate and at least one set of antibody fragments, wherein antibody fragments of each set include at least two types of separate antibody fragments, the at least two types of separate antibody fragments include at least one type of labeled antibody fragment having a labeled site labeled with a luminescent substance and at least one type of acceptor antibody fragment having an acceptor site for accepting emission from the labeled antibody fragment, and the at least one type of labeled antibody fragment and the at least one type of acceptor antibody fragment are capable of cooperatively recognizing one type of antigen in combination and are independently immobilized on the substrate in a positional relationship that allows each of the antibody fragments in one set to bind to the same antigen.09-29-2011
20120142559BIOMARKERS AND PARAMETERS FOR HYPERTENSIVE DISORDERS OF PREGNANCY - The application discloses new test panels comprising biomarkers and clinical parameters, for the prediction, diagnosis, prognosis and/or monitoring of hypertensive disorders of pregnancy and particularly preeclampsia; and related methods, uses, kits and devices.06-07-2012
20100298169METHOD OF PATTERNING MOLECULES ON A SUBSTRATE USING A MICRO-CONTACT PRINTING PROCESS - The present invention relates to a method of patterning molecules on a substrate using a micro-contact printing process, to a substrate produced by said method and to uses of said substrate. It also relates to a device for performing the method according to the present invention.11-25-2010
20100179073Immunologic assay for detection of autoantibodies to folate binding protein - The present invention is directed to an assay that detects autoantibodies to folate receptor and can be used in the clinical diagnostic testing of these autoantibodies in humans. Although there are other methods that exist to detect these autoantibodies, the assay described in the present invention has several features that offer advantages over the existing methods. Some of these features include adaptability to high-throughput processing, the use of an immunoglobulin antibody to bind autoantibodies bound to folate receptor or the use of enzyme-labeled folic acid to bind folate binding protein and use of fluorescence or chemiluminescence for detection. This assay thereby avoids the use of radioactivity and can be automated and scaled to process hundreds of samples safely and simultaneously.07-15-2010
20100210478MAKE AND USE OF SURFACE MOLECULES OF VARIED DENSITIES - The present invention relates to quantitative and quantity aspects of array synthesis and array uses as a device for high capacity producing synthetic molecules for off-array surface applications and as an assay device for on-array surface applications.08-19-2010
20120245057WHOLE PROTEOME TILING MICROARRAYS - The present invention relates to a microarray comprising at least 50,000 oligopeptide features per cm09-27-2012
20090215650SUBSTRATES WITH STABLE SURFACE CHEMISTRY FOR BIOLOGICAL MEMBRANE ARRAYS AND METHOD FOR FABRICATING THEREOF - The present invention provides a method for preparing a physically stable array of biological membranes, including membrane proteins, on a surface, and the resultant article of manufacture. The method comprises providing a substrate; creating either a polar surface or reactive surface by coating the substrate with a material that either: (1) enhances the stability of lipid spots during withdrawing through a water/air interface and washing and drying protocols; or (2) gives rise to minimal non-specific binding of a labeled target to a background surface, and high specific binding to a probe receptor in said membrane array, or (3) both; and depositing an array of biological-membrane microspots on the substrate. The method may further comprise applying a reagent that includes a soluable protein to stabilize the biological membranes on the surface. Also provided is an article having biological-membrane microspots that are associated in a stable fashion with a substrate surface embodying these properties.08-27-2009
20110071054Panel of monoclonal antibody containing pharmaceutical compositions - Methods, compositions, and kits relating to selecting a prophylactic or therapeutic antibody less likely to induce or aggravate an anti-antibody response in a subject administered the antibody. An antibody for administration to a subject may be selected to match, or at least more closely resemble, the allotypic phenotype of the subject's endogenous antibodies.03-24-2011
20100120634COMPATIBLE DISPLAY VECTOR SYSTEMS - The present invention provides a polynucleotide vector system used during polypeptide display that can be used to facilitate transfer of pools of polynucleotides encoding antigen binding proteins of interest. The present invention also provides methods that allow seamless conversion of pools of polynucleotides encoding antigen binding proteins using a restriction enzyme digestion and ligation strategy.05-13-2010
20120202713CONSTRUCTS AND LIBRARIES COMPRISING ANTIBODY SURROGATE LIGHT CHAIN SEQUENCES - The invention concerns constructs and libraries comprising antibody surrogate light chain sequences. In particular, the invention concerns constructs comprising VpreB sequences, optionally partnered with another polypeptide, such as, for example, antibody heavy chain variable domain sequences, and libraries containing the same.08-09-2012
20080242559Protein and peptide arrays - Ultrahigh resolution patterning, preferably carried out by DIP PEN™ nanolithographic printing, can be used to construct peptide and protein nanoarrays with nanometer-level dimensions. The peptide and protein nanoarrays, for example, exhibit almost no detectable nonspecific binding of proteins to their passivated portions. This work demonstrates how DIP PEN™ nanolithographic printing can be used in a method to generate high density protein and peptide patterns, which exhibit bioactivity and virtually no non-specific adsorption. It also shows that one can use AFM-based screening procedures to study the reactivity of the features that comprise such nanoarrays. The method encompasses a wide range of protein and peptide structures including, for example, enzymes and antibodies. Features at or below 300 nm can be achieved. In a preferred embodiment, parallel printing with multipen systems are used.10-02-2008
20110028349Endothelial monocyte activation polypeptide II, a biomarker for use in diagnosis and treatment of brain injury - A diagnostic tool and method of diagnosing brain injury and brain injury type (traumatic vs. ischemic) by detecting the level of expression of endothelial monocyte-activating polypeptide II (EMAP-II) and comparing to a control. An increase of EMAP-II indicates the presence of traumatic brain injury and a decrease of EMAP-II indicates the presence of ischemic brain injury. Detection of EMAP-II can be done in brain tissue, biofluids such as cerebrospinal fluid or blood (including plasma and serum)02-03-2011
20110257043ULTRA HIGH-THROUGHPUT OPTI-NANOPORE DNA READOUT PLATFORM - Described herein are methods for analyzing polymer molecules. These methods are employed for the high throughput readout of DNA and RNA molecules with single molecule sensitivity. The method of the present invention comprises (1) the electrically controlled unzipping of DNA (or RNA) double strands, and (2) the readout of the molecule's identity (or code) using one or more molecule signal detection.10-20-2011
20110136695UNIVERSAL LIBRARIES FOR IMMUNOGLOBULIN - Libraries of immunoglobulins of interest are described, the libraries containing mutated immunoglobulins of interest in which a single predetermined amino acid has been substituted in one or more positions in one or more complementarity-determining regions of the immunoglobulin of interest. The libraries comprise a series of subset libraries, in which the predetermined amino acid is “walked through” each of the six complementarity-determining regions (CDRs) of the immunoglobulin of interest not only individually but also for each of the possible combinatorial variations of the CDRs, resulting in subset libraries that include mutated immunoglobulins having the predetermined amino acid at one or more positions in each CDR, and collectively having the predetermined amino acid at each position in each CDR. The invention is further drawn to universal libraries containing one such library for each naturally-occurring amino acid as the single predetermined amino acid, totaling twenty libraries; and also to libraries of nucleic acids encoding the described libraries.06-09-2011
20110177977ANTIBODIES FOR DETECTING OR MONITORING A MALIGNANT PLASMA CELL DISEASE - The present invention is directed to antibodies having specificity for a heavy chain class at the same time as having specificity for a first light chain. Such antibodies can be used in a method of detecting or monitoring a malignant plasma cell disease comprising determining in a sample the ratio between the relative amounts of immunoglobulins having: 07-21-2011
20120302464METHODS FOR PRODUCING RECOMBINANT PROTEINS - Methods for producing recombinant cell populations are disclosed. The disclosed methods may be used to produce therapeutic polyclonal proteins.11-29-2012
20090176664High density peptide arrays containing kinase or phosphatase substrates - Peptide arrays and uses thereof for diagnostics, therapeutics and research. Ultra high density peptide arrays are generated using photolithography, such as using photoresist techniques.07-09-2009
20110190168APOPTOSIS MODULATOR BCL-B AND METHODS FOR MAKING AND USING SAME - A novel human member of the Bcl-2 family Bcl-B has been identified, which is Closest in amino-acid sequence homology to the Boo (Diva) protein. The Bcl-B protein is Widely expressed in adult human tissues. The Bcl-B protein modulates apoptosis. Bcl-B also binds Bcl-2, BCl-XL, and Bax but not Bak. Bcl-B displays a unique pattern of selectivity for binding and regulating the function of other members of the Bcl-2 family.08-04-2011
20100022414Droplet Libraries - The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.01-28-2010
20100160182Metal Ion-Based Immobilization - A method for immobilizing unmodified material using a metal-ion approach is provided wherein the material is immobilized on a surface in an active state on surface features coupled with metal-ions.06-24-2010
20090143247Methods for Producing Active scFv Antibodies and Libraries Therefor - The present disclosure describes scFv antibody libraries, antibodies isolated from the libraries, and methods of producing and using the same.06-04-2009
20100190661SERS-ACTIVE STRUCTURE FOR USE IN RAMAN SPECTROSCOPY - A surface-enhanced Raman spectroscopy (SERS)—active structure for use in Raman scattering detection has an array of nanostructures formed on a substrate by deposition and chemical etching. The nanostructures are coated with metal nanoparticles.07-29-2010
20110065608Devices for Detecting Renal Disorders - Devices for diagnosing, monitoring, or determining a renal disorder in a mammal are described. In particular, devices for diagnosing, monitoring, or determining a renal disorder using measured concentrations of a combination of three or more analytes in a test sample taken from the mammal are described.03-17-2011
20110065609Methods and Kits for the Diagnosis of Rheumatoid Arthritis - The present invention relates to the identification and use of proteins with clinical relevance to rheumatoid arthritis (RA). In particular, the invention provides the identity of marker proteins that specifically react with RA-associated autoantibodies. Also provided are methods, arrays and kits for using these proteins in the diagnosis of RA, and in the selection and/or monitoring of treatment regimens.03-17-2011
20110111985Biosensor - The invention provides a product comprising: a membrane-spanning protein; a lipid membrane formed from amphiphilic molecules and membrane-spanning protein molecules; and a substrate: characterised in that the membrane protein is directly coupled to the substrate. The invention also provides a method for producing such a product which i) comprises treating a substrate with a hydrophilic coating agent; providing at least one membrane-spanning protein; iii) bringing the protein into contact with the treated substrate under conditions for the coupling of the protein directly to the treated substrate; iv) adding amphiphilic molecules to the protein-coupled substrate to form a lipid membrane. The product is useful for biosensors, protein arrays and the like.05-12-2011
20120065107REAGENTS AND METHODS FOR USE IN CANCER DIAGNOSIS, CLASSIFICATION AND THERAPY - Methods and reagents for classifying tumors and for identifying new tumor classes and subclasses. Methods for correlating tumor class or subclass with therapeutic regimen or outcome, for identifying appropriate (new or known) therapies for particular classes or subclasses, and for predicting outcomes based on class or subclass. New therapeutic agents and methods for the treatment of cancer.03-15-2012
20120021954FORMATION OF ORGANIC NANOSTRUCTURE ARRAY - A nanostructure array is disclosed. The nanostructure array comprises a plurality of elongated organic nanostructures arranged generally perpendicularly to a plane.01-26-2012
20120122736SENSORS INCORPORATING ANTIBODIES AND METHODS OF MAKING AND USING THE SAME - A sensor comprising an electronic circuit electrically coupled to a type III-V semiconductor material, for example indium arsenide (InAs) and an antibody contacting the type III-V semiconductor material. The sensor produces measurable N changes in the electrical properties of the semiconductor upon antibody-antigen binding events. Electrical properties measurable by the electronic device may include resistivity, capacitance, impedance, and inductance. A method of detecting an antigen using sensors of the invention. A method of detecting a reaction of an analyte to a stimulus using sensors of the invention. Sensor arrays comprising multiple sensors of the invention.05-17-2012
20120108468Cell-based arrays, methods of making, and methods of using - Embodiments of the present disclosure provide for arrays, systems, and methods for the analyzing cells, methods of making arrays, and the like.05-03-2012
20120270752ANALYZING THE EXPRESSION OF BIOMARKERS IN CELLS WITH MOMENTS - Cell profile data is stored. The cell profile data comprises multiplexed biometric images capturing the expression of a plurality of biomarkers by at least one tissue sample from a patient with respect to at least one field of view. Individual cells are delineated and segmented into compartments. At least one cell feature is calculated based on the cell's expression of each of a plurality of biomarkers. A first moment is calculated for each cell feature. The moments are examined for an association with a diagnosis, a prognosis of a response to treatment of a condition or disease. Apparatus for performing the foregoing steps are disclosed.10-25-2012
20090131278NON-SPECIFIC BINDING RESISTANT PROTEIN ARRAYS AND METHODS FOR MAKING THE SAME - Arrays of protein-capture agents useful for the simultaneous detection of a plurality of proteins which are the expression products, or fragments thereof, of a cell or population of cells in an organism are provided. A variety of antibody arrays, in particular, are described. Methods of both making and using the arrays of protein-capture agents are also disclosed. The invention arrays are particularly useful for various proteomics applications including assessing patterns of protein expression and modification in cells.05-21-2009
20090131277PROSTATE CANCER DIAGNOSIS AND TREATMENT - The present invention relates to novel mimetopes of anti-PSMA antibodies and their use for detecting, imaging, staging, treating and monitoring of prostate cancer, and/or metastatis thereof. The present invention also relates to novel pharmaceutical compositions for the treatment of prostate cancer. Furthermore the present invention relates to assay systems and kits for detecting, imaging, staging, treating and monitoring of prostate cancer, and/or metastasis thereof.05-21-2009
20120316085ANTIBODY HUMANIZATION BY FRAMEWORK ASSEMBLY - An improved method for producing humanized antibody or an antigen binding fragment thereof is described. The method, designated framework-assembly, bypasses the reliance on structural biology and the construction of large libraries. It is easier to implement and more efficient than the rational design and empirical methods. Also described are humanized antibodies produced by the method and related framework-assembly library.12-13-2012
20110190169SIMULTANEOUS ASSAY FOR DETERMINING DRUGS - Bodily fluid is analyzed for the presence of drugs of a selected panel of drugs in a simultaneous assay in which sample of the fluid is incubated with additional amounts of all drugs of the panel, antibodies specific to each of the drugs of the panel, and microparticles, the microparticles being divided into subsets, one subset for each drug in the panel and each subset distinguishable from the others. The incubation is performed in a liquid medium in which competitive binding occurs, the drugs in the sample competing with those added to the assay medium for binding to the antibodies. In one procedure, the added drugs are pre-coupled to the microparticles while the antibodies are not, and the incubation is followed by further incubating the microparticles with labeled ligands that have affinity for the antibodies. In an alternative procedure, the added drugs are not coupled to the microparticles but are pre-labeled, while the antibodies are pre-coupled to the microparticles, and the assay proceeds without further incubation. In both alternatives, the microparticles are ultimately recovered from the assay medium and from any unbound species, and the recovered microparticles are analyzed by flow cytometry to obtain indications of the presence of the various drugs in the sample in an inverse manner by detection of the label, each drug differentiable from the others by the distinguishing features of the microparticles.08-04-2011
20120214710METHOD OF PREDICTING RESPONSE TO THALIDOMIDE IN MULTIPLE MYELOMA PATIENTS - A method of predicting response to thalidomide, or thalidomide analogs, in an individual with cancer, especially cancers for which thalidomide has been implicated as a treatment, such as Multiple Myeloma (MM) employs one or more of a panel of biomarkers that have been shown to be differentially expressed in cancer patients that respond to thalidomide (hereafter “Responders”) relative to cancer patients that do not respond to thalidomide (hereafter “Non-responders). The method involves assaying a biological sample from the individual to determine the abundance of at least three biomarkers including Vitamin-D binding protein precursor (VDB) (Sequence ID 1) and Serum amyloid A protein (SAA) (Sequence ID 3), and at least one of beta-2-microglobulin (B2M) (Sequence ID 4), Haptoglobin (Hp) precursor (fragment) (Sequence ID 5), and zinc-alpha-2-glycoprotein (ZAG) (Sequence ID 2). Correlation of the abundance value for the at least three biomarkers with a reference abundance value from a Responder or Non-responder enables predication of response to thalidomide for the patient.08-23-2012
20120264652DIRECTED HETEROBIFUNCTIONAL LINKERS - A heterobifunctional linker that comprises (a) a moiety capable of reversibly coupling the linker to a selected epitope of a target molecule; and (b) a moiety or moieties capable of irreversibly coupling the linker to the target, and optionally a moiety or moieties capable of irreversibly coupling the linker to a solid surface. Also described is a method of orienting molecules, such as proteins, with respect to a surface and to each other, employing such a linker, and an array of such oriented molecules, which may be the same or different.10-18-2012
20110046016DISPOSABLE REACTION VESSEL WITH INTEGRATED OPTICAL ELEMENTS - The present invention provides disposable, semi-reusable, or single use reaction vessels with integrated optical elements for use with diffraction based assay systems. The vessel for assaying liquids for analytes includes a housing having at least one chamber or well for receiving a liquid therein and an optical element integrally formed with the housing for directing an incident light beam towards the well or chamber and directing a light beam away from the chamber after the light beam has interacted with analytes present in the liquid. The vessel may be test tube such as a blood collection tube, with or without, an optical element but having a pattern of analyte-specific receptors located on an inner surface of the tube wall so that when a liquid is introduced into the interior of the test tube analytes present in the liquid can bind with the pattern of analyte-specific receptors.02-24-2011
20110046015PEPTIDE IMMOBILIZATION SOLUTION AND USE THEREOF - Disclosed is a peptide immobilization technique which can achieve the immobilization of a satisfactory quantity of a peptide on a solid support. In the immobilization of a peptide on a solid support, a surfactant is allowed to coexist on the solid support together with the peptide. In this manner, the quantity of the peptide immobilized on the solid support can be increased.02-24-2011
20100234246METHOD FOR PRODUCING PEPTIDE LIBRARIES AND USE THEREOF - Screening libraries of peptides in different assays offers an opportunity to simultaneously interrogate intracellular signaling pathways, create reagents to further the understanding of the pathway, and to create novel forms of therapies.09-16-2010
20120277122GFABS: GFP-Based Biosensors Possessing the Binding Properties of Antibodies - A family of GFP scaffolds capable of accommodating two proximal, randomized binding loops is disclosed. GFP-based binders binding with nanomolar affinity are developed from a library of these GFP scaffolds.11-01-2012
20130012415COMPOSITIONS AND METHODS FOR DIAGNOSIS, PROGNOSIS AND MANAGEMENT OF MALARIA - Biomarkers for predicting the severity of malaria and methods for their detection are disclosed. In one aspect, the present application discloses CXCL4, CXCL10, VEGF, PGDF, IL-1Ra, IL-8, MIP-1β, sFas, Fas-L, sTNF-R2, and sTNF-R1 as biomarkers for the severity of malaria. In another aspect, the present application discloses a method for determining the severity of malaria and predicting mortality due to cerebral malaria. The method comprises the detection of the biomarkers CXCL4 and/or CXCL10 and at least one more biomarker and determining the severity of malaria and predicting mortality due to cerebral malaria based upon the ratio of expression of the biomarkers in the subject versus the expression of the biomarkers in a control.01-10-2013
20100130382Liposome, Proteoliposome, Biochip, and Method for Producing Liposome and Proteoliposome - A liposome comprising a region of a lipid bilayer membrane with different membrane thicknesses, wherein each lipid bilayer membrane region is composed of a different lipid, and a thick membrane side in the region of the lipid bilayer membrane is formed of lipid having a phase transition temperature higher than that of the lipid forming a thin membrane side in the region of the lipid bilayer membrane. A proteoliposome, wherein the above-described liposome includes membrane proteins. A biochip, wherein the above-described liposome or the above-described proteoliposome is spread on a substrate. The above-described biochip, wherein the substrate includes at least one kind selected from the group consisting of mica, SiO05-27-2010
20120149600MICROFLUIDIC DEVICES AND METHODS - Contemplated microfluidic devices and methods are drawn to protein arrays in which distinct and detergent-containing antigen preparations are deposited onto an optical contrast layer in a non-specific and non-covalent manner. Detection of binding a is carried out using a dye that precipitates or agglomerates to so form a visually detectable signal at a dynamic range of at least three orders of magnitude.06-14-2012
20080220988Preparing carbohydrate microarrays and conjugated nanoparticles - The present invention is directed to carbohydrate microarray and conjugated nanoparticles methods of making the same.09-11-2008
20130130939PORTABLE PHOTONIC SENSOR SYSTEM AS AN EARLY DETECTION TOOL FOR OVARIAN CANCER - A guided mode resonance (GMR) sensor that can be used to simultaneously detect an array of analytes. It provides a diagnostic system that can rapidly detect an array of biomarker proteins in patient samples (such as blood, serum or plasma for example) which can be used as an accurate means to conduct a differential analysis of proteins that allows the discrimination of early and late stages of disease, such as metastatic versus primary ovarian serous carcinomas. The GMR sensor can be provided in a compact size such that it can be portable.05-23-2013
20080200348Polysaccharide structure and sequence determination - The invention provides a method for the structural analysis of a saccharide, comprising: a) providing on a surface a plurality of essentially sequence- and/or site-specific binding agents; b) contacting said surface with a saccharide to be analyzed, or with a mixture comprising a plurality of fragments of said saccharide; c) washing or otherwise removing unbound saccharide or saccharide fragments; d) adding to the surface obtained in step c) an essentially sequence- and/or site-specific marker, or a mixture of essentially sequence- and/or site-specific markers; e) acquiring one or more images of the markers that are bound to said surface; and f) deriving information related to the identity of the saccharide being analyzed from said image.08-21-2008
20110224102Eukaryotic signal sequences for polypeptide expression and polypeptide display libraries - The present invention generally relates to methods and compositions for expressing proteins or polypeptides in prokaryotic hosts using eukaryotic signal sequences.09-15-2011
20110275542SIGNATURES AND DETERMINANTS FOR DISTINGUISHING BETWEEN A BACTERIAL AND VIRAL INFECTION AND METHODS OF USE THEREOF - The present invention provides methods of detecting infection using biomarkers.11-10-2011
20130150264Methods, Kits and Compositions Pertaining to the Suppression of the Detectable Probe Binding to Randomly Distributed Repeat Sequences Genomic Nucleic Acid - This invention is directed to methods, kits, non-nucleotide probes as well as other compositions pertaining to the suppression of binding of detectable nucleic acid probes to undesired nucleotide sequences of genomic nucleic acid in assays designed to determine target genomic nucleic acid.06-13-2013
20100292104Photoswitchable Method for the Ordered Attachment of Proteins to Surfaces - Described herein is a method for the attachment of proteins to any solid support with control over the orientation of the attachment. The method is extremely efficient, not requiring the previous purification of the protein to be attached, and can be activated by UV-light. Spatially addressable arrays of multiple protein components can be generated by using standard photolithographic techniques.11-18-2010
20120258890NS1-NP Diagnostics of Influenza Virus Infection - The present application describes methods for assessing influenza infection, including prognosis. An assay that determines the amount of the NS1 and NP proteins of influenza virus shows enhanced sensitivity and reliability compared to either antigen alone. Many formats employ pan-specific antibodies (i.e., that react with all or at least with multiple strains within an influenza type).10-11-2012
20120283143Compositions and methods for antibody and ligand identification - Embodiments disclosed herein relate to methodology, and kits thereof for identifying particular disease-associated antibodies partially based on comparative binding to a mimotope array. Isolation of identified disease-associated antibodies and uses thereof are also described.11-08-2012
20130157900STRUCTURE-ACTIVITY RELATIONSHIPS - The present disclosure relates to compositions and methods for screening a plurality of polypeptide variants.06-20-2013
20120094874STABILIZED IMMUNOGLOBULIN CONSTANT DOMAINS - The invention refers to a multidomain modular antibody comprising at least one constant antibody domain, which is mutated to form an artificial disulfide bridge by introducing at least one Cys residue into the amino acid sequence through mutagenesis of said constant domain to obtain an intra-domain or inter-domain disulfide bridge within the framework region, libraries based on such antibodies and methods of producing.04-19-2012
20130210678MOESIN FRAGMENTS AND USES THEREOF - The present invention provides compositions and methods useful for detecting and monitoring autoimmune diseases.08-15-2013
20130210677REAGENTS AND METHODS FOR USE IN CANCER DIAGNOSIS, CLASSIFICATION AND THERAPY - Methods and reagents for classifying tumors and for identifying new tumor classes and subclasses. Methods for correlating tumor class or subclass with therapeutic regimen or outcome, for identifying appropriate (new or known) therapies for particular classes or subclasses, and for predicting outcomes based on class or subclass. New therapeutic agents and methods for the treatment of cancer.08-15-2013

Patent applications in class Peptides or polypeptides, or derivatives thereof