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By measuring a physical property (e.g., mass, etc.)

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506 - Combinatorial chemistry technology: method, library, apparatus


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20120184460HIGHLY EFFICIENT GENE-REGULATORY ELEMENT SCREENING ASSAY AND COMPOSITIONS FOR PERFORMING THE SAME - Methods of evaluating a gene-regulatory element, including libraries of known or candidate gene-regulatory elements, are provided. Aspects of the methods include cytometrically analyzing a cell comprising a gene-regulatory element construct, e.g., a plasmid, having an activity reporter comprising a first signal reporter domain which produces a first signal operatively coupled to a gene-regulatory element of interest; and a noise reporter comprising a second signal reporter which produces a second signal that is distinguishable from the first signal to obtain the first and second signals. The first signal is then normalized with the second signal to obtain a normalized activity signal, which normalized activity signal is then employed to evaluate the gene-regulatory element. Also provided are reagents, systems and kits that find use in practicing methods of the invention.07-19-2012
20130079247METHOD AND APPARATUS FOR DELIVERY OF SUBMICROLITER VOLUMES ONTO A SUBSTRATE - A slotted pin tool, a delivery system containing the pin tool, a substrate for use in the system and methods using the pin tool and system are provided. The slotted pin tool contains a plurality of pins having slotted ends designed to fit around each loci of material deposited on a surface, such as a microarray, without contacting any of the deposited material. Sample is delivered by contacting the pin tool with the surface; the amount delivered is proportional to the velocity of the pin tool as it contacts the surface or the velocity of the liquid when movement of the pin is halted.03-28-2013
20100035767POLYMERASE - The present invention relates to an engineered polymerase with an expanded substrate range characterized in that the polymerase is capable of incorporating an enhanced occurrence of detection agent-labeled nucleotide analogue into nucleic acid synthesized by that engineered polymerase as compared with the wild type polymerase from which it is derived.02-11-2010
20130040857MASS SPECTROMETRIC ASSAYS FOR PEPTIDES - Methods for interpretation of mass spectrometric tests for clinical biomarkers in which the amounts of internal standards are set to equal clinical evaluation thresholds, and preparations for adding stable isotope labeled peptide species to sample digests while minimizing losses and alterations in peptide stoichiometry.02-14-2013
20090054262DEVICES FOR CELL ASSAYS - The present invention relates to the field of molecular diagnostics. In particular, the present invention provided improved substrates and methods of using liquid crystals and other biophotonically based assays for quantitating the amount of an analyte in a sample. The present invention also provides materials and methods for detecting non-specific binding of an analyte to a substrate by using a liquid crystal or other biophotonically based assay formats.02-26-2009
20090176661WHOLE GENOME EXPRESSION ANALYSIS SYSTEM - A method for simultaneously determining a genetic expression profile for an individual member of a species relative to an entire standard genome for the species. The method can comprise distributing a liquid sample into an array of reaction chambers of a substrate. The array can comprise a primer set and a probe for each polynucleotide target along the entire standard genome. The liquid sample can comprise substantially all genetic material of the member. Each of the reaction chambers can comprise the primer set and the probe for at least one of the polynucleotide targets and a polymerase. The method can further comprise amplifying the liquid sample in the array, detecting a signal emitted by at least one of the probes, and identifying the genetic expression profile in response to the signal.07-09-2009
20090156429PULSED-MULTILINE EXCITATION FOR COLOR-BLIND FLUORESCENCE DETECTION - The present invention provides a technology called Pulse-Multiline Excitation or PME. This technology provides a novel approach to fluorescence detection with application for high-throughput identification of informative SNPs, which could lead to more accurate diagnosis of inherited disease, better prognosis of risk susceptibilities, or identification of sporadic mutations. The PME technology has two main advantages that significantly increase fluorescence sensitivity: (1) optimal excitation of all fluorophores in the genomic assay and (2) “color-blind” detection, which collects considerably more light than standard wavelength resolved detection. Successful implementation of the PME technology will have broad application for routine usage in clinical diagnostics, forensics, and general sequencing methodologies and will have the capability, flexibility, and portability of targeted sequence variation assays for a large majority of the population.06-18-2009
20090156428MULTI-MODE MICROARRAY APPARATUS AND METHOD FOR CONCURRENT AND SEQUENTIAL BIOLOGICAL ASSAYS - A multi-mode, multiplexed array of probe elements of various forms attached to a common support and methods of using the array are disclosed. The array is used for the concurrent and/or sequential detection of combinations of more than one chemical and/or biological material, such as: target nucleic acid sequences, genomic DNA, pcr products, RNA (including microRNA), single nucleotide polymorphisms, proteins, peptides, carbohydrates, polysaccharides, phosphorylation or methylation state of target molecules, chromosomal abnormalities, and other biomolecules, moieties, metals, or chemical compounds in a biological sample. The method of the invention includes the deposition and/or in-situ synthesis of the various probe elements which comprise the array or group of arrays, the preparation and delivery of the target biological sample in forms suitable for concurrent and/or sequential detection, the hybridization methods of target to probe, the detection methods for target-probe interactions, and the use of such arrays in automated systems for research or diagnostic applications.06-18-2009
20100105575SINGLE NUCLEOTIDE POLYMORPHISM GENOTYPING DETECTION VIA THE REAL-TIME INVADER ASSAY MICROARRAY PLATFORM - A method and apparatus for real-time, simultaneous, quantitative measurement for detecting a single nucleotide polymorphism in a target nucleic acid is provided. This method involves combining a polymerase chain reaction (PCR) technique with invader assay technique.04-29-2010
20100069259SAMPLE DEVICE PRESERVATION - A method for archiving sample devices such as microarray slides and membranes is described using an optically clear, solidifying solution. Also described are related methods and kits.03-18-2010
20090042739CELLOMICS SYSTEM - In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.02-12-2009
20090093375DNA or RNA detection and/or quantification using spectroscopic shifts or two or more optical cavities - A spectroscopic technique for high-sensitivity, label free DNA quantification uses a shift in an optical resonance (whispering gallery mode, WGM) excited in a micron-sized optical cavity (e.g., a silica sphere) to detect and measure nucleic acids. The surface of the silica sphere is chemically modified with oligonucleotides. Hybridization to the target DNA leads to a red-shift of the optical resonance wavelength. The sensitivity of this resonance technique is higher than most optical single-pass devices such as surface plasmon resonance biosensors. Each microsphere can be identified by its unique resonance wavelength. Specific, multiplexed DNA detection may be provided by using two or more microspheres. The multiplexed signal from two or more microspheres illustrates that a single nucleotide mismatch in an 11-mer oligonucleotide can be discriminated with a high signal-to-noise of 54. This all-photonic WGM biosensor can be integrated on a chip, such as a semiconductor chip, which makes it an easy to manufacture, analytic component for a portable, robust lab-on-a-chip device.04-09-2009
20130059758Detection, Staging and Grading of Benign and Malignant Tumors - The present invention provides a method for detecting and grading benign and malignant tumors using at least one sensor of conductive nanoparticles capped with an organic coating in conjunction with a learning and pattern recognition algorithm. The method utilizes a plurality of response induced parameters to obtain improved sensitivity and selectivity for diagnosis, prognosis, monitoring and staging various types of cancers, or for identifying or grading benign or malignant tumors.03-07-2013
20090270275METHOD AND APPARATUS FOR SCANNING BIO CHIPS USING LIGHT AMPLICATION BY METAL NANO-PARTICLES - The present invention utilizes a principle in which, in a state in which metal nano-particles are attached to target probes and used as markers, and the metal nano-particles have a proper density according to a bio reaction between the target probes and fixed probes, when the metal nano-particles are irradiated with a laser beam having a proper intensity from the optical pick-up head, a higher optical energy is delivered to the phase change layer by an optical amplification effect caused by the metal nano-particles, thereby better inducing an amorphous-to-crystalline phase change.10-29-2009
20090075838Biological Analysis Arrangement and Approach Therefor - Characteristics of a chemical or biological sample are detected using an approach involving light detection. According to an example embodiment of the present invention, an assaying arrangement including a light detector is adapted to detect light from a sample, such as a biological material. A signal corresponding to the detected light is used to characterize the sample, for example, by detecting a light-related property thereof. In one implementation, the assaying arrangement includes integrated circuitry having a light detector and a programmable processor, with the light detector generating a signal corresponding to the light and sending the signal to the processor. The processor provides an output corresponding to the signal and indicative of a characteristic of the sample.03-19-2009
20120115755MICROFLUIDIC MAGNETOPHORETIC DEVICE AND METHODS FOR USING THE SAME - A microfluidic device may employ one or more sorting stations for separating target species from other species in a sample. The separation is driven by magnetophoresis. A sorting station generally includes separate buffer and sample streams. A magnetic field gradient applied to the sorting station deflects the flow path of magnetic particles (which selectively label the target species) from a sample stream into a buffer stream. The buffer stream leaving the sorting station is used to detect or further process purified target species labeled with the magnetic particles.05-10-2012
20090054261METHOD - A method of controlling a system having a microfluidic channel structure in which fluids are able to interact to produce at least one product comprises the steps of: 02-26-2009
20090011952Chip assay having improved efficiency - The chip includes electrodes on a substrate. The electrodes include a working electrode, a reference electrode, and a counter electrode. The reference electrode is constructed so as to not have an intrinsic potential. A self-assembly monolayer is positioned on the reference electrode. The self-assembly monolayer includes spacers and active probes. The active probes are configured to have a higher affinity for a capture probe than the spacers have for the capture probe.01-08-2009
20080300146Bio Chip Device with a Sample Compartment and a Light Sensitive Element, Method for the Detection of Fluorescent Particles Within at Least One Sample Compartment of a Bio Chip Device - The invention provides a bio chip device comprising at least one sample compartment and at least one light sensitive element, the at least one sample compartment being provided on a first side of the at least one light sensitive element, wherein incident light is provided incident from a second side opposite of the first side of the at least one light sensitive element. Further, the invention provides a method for the detection of fluorescent particles within at least one sample compartment of a bio chip device.12-04-2008
20120238472BIOANALYTICAL INSTRUMENTATION USING A LIGHT SOURCE SUBSYSTEM - The invention relates to a light source for irradiating molecules present in a detection volume with one or more selected wavelengths of light and directing the fluorescence, absorbance, transmittance, scattering onto one or more detectors. Molecular interactions with the light allow for the identification and quantitation of participating chemical moieties in reactions utilizing physical or chemical tags, most typically fluorescent and chromophore labels. The invention can also use the light source to separately and simultaneously irradiate a plurality of capillaries or other flow confining structures with one or more selected wavelengths of light and separately and simultaneously detect fluorescence produced within the capillaries or other flow confining structures. In various embodiments, the flow confining structures can allow separation or transportation of molecules and include capillary, micro bore and milli bore flow systems. The capillaries are used to separate molecules that are chemically tagged with appropriate fluorescent or chromophore groups.09-20-2012
20120238471Ultrasensitive Biochemical Sensing Device and Method of Sensing Analytes - Systems and methods biochemically sense a concentration of a ligand using a sensor having a substrate having a metallic nanoparticle array formed onto a surface of the substrate. A light source is incident on the surface. A matrix is deposited over the nanoparticle array and contains a protein adapted to binding the ligand. A detector detects s-polarized and p-polarized light from the reflective surface. Spacing of nanoparticles in the array and wavelength of light are selected such that plasmon resonance occurs with an isotropic point such that −s and −p polarizations of the incident light result in substantially identical surface Plasmon resonance, wherein binding of the ligand to the protein shifts the resonance such that differences between the −S and −P polarizations give in a signal indicative of presence of the ligand.09-20-2012
20130165346SYSTEM AND METHOD FOR SCREENING A LIBRARY OF SAMPLES - A continuous throughput microfluidic system includes an input system configured to provide a sequential stream of sample plugs; a droplet generator arranged in fluid connection with the input system to receive the sequential stream of sample plugs and configured to provide an output stream of droplets; a droplet treatment system arranged in fluid connection with the droplet generator to receive the output stream of droplets in a sequential order and configured to provide a stream of treated droplets in the sequential order; a detection system arranged to obtain detection signals from the treated droplets in the sequential order; a control system configured to communicate with the input system, the droplet generator, and the droplet treatment system; and a data processing and storage system configured to communicate with the control system and the detection system.06-27-2013
20100331212METHODS AND SYSTEMS FOR MONITORING MULTIPLE OPTICAL SIGNALS FROM A SINGLE SOURCE - Methods and systems are described for determining information about template sequences by simultaneously providing excitation over time to a plurality of confined sources on a substrate, each confined source carrying out a template mediated addition of nucleotides with a polymerase enzyme. The nucleotides have different labels, each with a different emission wavelength profile. Optical elements direct optical signals from the different NTPs within each optical confinement to different detector locations. Observing the optical signals over time allows for obtaining sequence information about the template nucleic acids.12-30-2010
20110111982MAGNETIC PARTICLES - A magnetic particle comprises a polysaccharide matrix and a plurality of magnetic crystals dispersed in the matrix. A method for making magnetic particles comprises combining a basic solution with a metal ion solution and allowing the metal ions to oxidize to form magnetic crystals, and combining the magnetic crystals with a polysaccharide solution to form the magnetic particles.05-12-2011
20130023444COLOR-PRODUCING DIAGNOSTIC SYSTEMS, REAGENTS AND METHODS - Provided herein are novel reagents and their use in color-producing detection systems for performing diagnostic tests and analytical assays.01-24-2013
20110301063MULTIPLEXING DERIVATIZED ANAYLTES USING MASS SPECTROSCOPY - This document relates to methods and materials involved in simultaneously determining (i.e., multiplexing) the levels of an analyte in biological samples from multiple subjects using high pressure liquid chromatography-tandem mass spectrometry (LC-MS/MS). For example, methods and materials for derivatizing vitamin D metabolites in samples obtained from multiple subjects (e.g., humans), and combining samples for simultaneous analysis in a single assay are provided.12-08-2011
20110301062RELIABLE FLUORESCENCE CORRECTION METHOD FOR TWO-COLOR MEASUREMENT FLUORESCENCE SYSTEM - Methods and devices use in two-color measurement systems. The methods and devices include methods of making corrections, methods of calculating correction factors, fluorescence scanners, and microarray chips. The said methods and devices enable a user to correct fluorescence intensities for errors caused by the occurrence of FRET and /or cross-talk when two fluorophores are used in two-color fluorescence arrays.12-08-2011
20110301061HIGH THROUGHPUT EXPERIMENTATION METHODS FOR PHASE SEPARATION - A process for testing the effectiveness of demulsifying additives on oil/water emulsions includes adding samples containing differing combinations of oil, water and demulsifier to a plurality of elongate reactor vials, wherein each the elongate reactor vial has a longitudinal axis extending from its bottom to its rim. The plurality of reactor vials are placed into a reaction block mounted on a platform of a shaker, wherein the reactor vials are received in stations of the reaction block in a vertical orientation such that the longitudinal axis of each reactor vial is perpendicular to the platform. The reaction block is pivoted the so that the longitudinal axis of each reactor vial is parallel with the platform in a horizontal orientation. The method further includes agitating the reactor vials with the shaker to simultaneously form an oil/water emulsion in each reactor vial while the reactor vials are in the horizontal orientation and then pivoting the reaction block to return the reactor vials to a vertical orientation. The demulsification of the oil/water emulsion in the plurality of reactor vials is observed with the reactor vials in the vertical orientation. In one embodiment, the method further includes using an imaging device to record the demulsification of the oil/water emulsion.12-08-2011
20110287976MICROFLUIDIC SOLUTION FOR HIGH-THROUGHPUT, DROPLET-BASED SINGLE MOLECULE ANALYSIS WITH LOW REAGENT CONSUMPTION - A microfluidic device for a confocal fluorescence detection system has an input channel defined by a body of the microfluidic device, a sample concentration section defined by the body of the microfluidic device and in fluid connection with the input channel, a mixing section defined by the body of the microfluidic device and in fluid connection with the concentration section, and a detection region that is at least partially transparent to illumination light of the confocal fluorescence detection system and at least partially transparent to fluorescent light when emitted from a sample under observation as the sample flows through the detection region.11-24-2011
20110287975Methods and Compositions for Correlating Genetic Markers with Conversion of Medium Chain Polyunsaturated Fatty Acids to Long Chain Polyunsaturated Fatty Acids - The present invention provides methods of identifying a subject having an increased or decreased ability to convert medium chain-polyunsaturated fatty acids (MC-PUFAs) to long chain polyunsaturated fatty acids (LC-PUFAs), comprising: (a) correlating the presence of one or more than one genetic marker in chromosome 11ql2-13, between build 37.1 position 61548559 and build 37.1 position 61560261, with an increased ability to convert MC-PUFAs to LC-PUFAs; and b) detecting the one or more than one genetic marker of step (a) in the subject, thereby identifying the subject as having an increased ability to convert MC-PUFAs to LC-PUFAs. Also provided are methods of correlating one or more genetic markers with an ability to convert MC-PUFAs to LC-PUFAs.11-24-2011
20110294700HIGH-THROUGHPUT QUANTITATION OF CROP SEED PROTEINS - The invention provides a high-throughput method for quantitating plant seed proteins, e.g. seed allergens. Such method involves obtaining a protein sample from the seed; analyzing the sample using mass spectrometry with a set of pre-designed internal standard peptides that are specific to the plant allergens; and monitoring frequencies and intensities of resulting spectra to obtain relative and absolute allergen contents in the seed. The invention also provides a system for high-throughput profiling of plant seed allergens. Such system comprises a set of pre-designed internal standard peptides that are specific to the plant seed allergens and one or more mass spectrometers.12-01-2011
20110294699METHODS AND COMPOSITIONS FOR UNIVERSAL SIZE-SPECIFIC PCR - Provided herein are products and processes for the amplification, detection and sequencing of short-stranded nucleic acid in the presence of a high background of long-stranded genomic material (e.g., host or maternal nucleic acids). The methods rely on the use of inside and outside primers introduced at varying concentrations, as well as universal amplification reactions that preferentially amplify short, low copy number nucleic acid.12-01-2011
20100216667METHOD FOR DETERMINING COMPATIBILITY OF AN ACTIVE PHARMACEUTICAL INGREDIENT WITH MATERIALS - Disclosed is a system or matrix approach for determining compatibility of a pharmaceutically active substance, such as small molecule drug candidate, therapeutic proteins, peptides, vaccines or RNAi, with materials used in the research and development of pharmaceuticals, including plastics, polymers, resins, rubbers, elastomers, glass and steel.08-26-2010
20090318307DEVICE FOR MOLECULAR DIAGNOSIS - The present invention relates to biological detection devices wherein melting curve analysis is performed an electrical sensor and a programmable heating element. The device optionally further comprises means for optically detecting nucleic acids within the device.12-24-2009
20110195866AUTOMATED MULTI-STATION SMALL OBJECT ANALYSIS - The present disclosure provides systems and methods for analyzing a plurality of samples of small objects. In various other embodiments, the system (08-11-2011
20090149345MICROCHANNEL ARRAY AND METHOD FOR PRODUCING THE SAME, AND BLOOD MEASURING METHOD EMPLOYING IT - A microchannel array, a method of manufacturing the same, and a blood test method. The microchannel array is formed by joining first and second substrates, each including a fluid inlet and outlet on their surfaces. An internal space structure connects the fluid inlet and outlet, and includes an upstream flow channel connected with the fluid inlet, a downstream flow channel connected with the fluid outlet with a gap therebetween, and a micro flow channel connecting the upstream and downstream flow channels. A minimum distance from a center of a sectional surface of the micro flow channel to a side wall of the micro flow channel is smaller than that of the upstream and downstream flow channels. Each surface of the first and second substrates includes grooves for creating the upstream and downstream flow channels, and the surface of the second substrate has grooves for creating the micro flow channel.06-11-2009
20090149344SURFACE ENHANCED RAMAN SPECTROSCOPY (SERS) SYSTEMS AND METHODS OF USE THEREOF - Surface-enhanced Raman spectroscopic (SERS) systems and methods for detecting biomolecules of interest, such as a virus, bacterium, or other infectious agent, are provided. A spectroscopic assay based on surface enhanced Raman scattering (SERS) using a silver nanorod array substrate fabricated by oblique angle deposition has been developed that allows for rapid detection of trace levels of viruses or bacteria with a high degree of sensitivity and specificity. This novel and improved SERS assay can detect minor spectral differences within strains of a single virus type such as respiratory syncytial virus or influenza virus in the presence of biological media. The method provides rapid diagnostics for direct molecular and structural characterization of virus strains and virus gene deletion mutants generating reproducible viral spectra without viral manipulation.06-11-2009
20090143244System and apparatus for sequential processing of analytes - An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.06-04-2009
20120035080OPTICAL LENS SYSTEM AND METHOD FOR MICROFLUIDIC DEVICES - An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.02-09-2012
20100120629Biomarkers for Differentiating Between Type 1 and Type 2 Diabetes - Biomarkers that are diagnostic of type 1 diabetes, type 2 diabetes and/or diabetic disorder are identified. Detection of different biomarkers of the invention are also diagnostic of the degree of severity of type 1 diabetes, type 2 diabetes and/or diabetic disorder. An analysis includes the parameters of matching for BMI and Tanner stage. Receiver-operator characteristic (ROC) curves were established to assess association of the biomarkers with a disease.05-13-2010
20100009868Self-Assembled Combinatorial Encoding Nanoarrays for Multiplexed Biosensing - The present invention provides combinatorial encoding nucleic acid tiling arrays and methods for their use and synthesis.01-14-2010
20100081582HIGH THROUGHPUT METHOD FOR MEASURING TOTAL FERMENTABLES - According to the invention, there is provided a high throughput method for measuring total fermentables using a small amount of plan tissue. A high throughput screening tool for gene discovery aiming for increasing total fermentables is further provided.04-01-2010
20090253589Method for Testing Active Compounds - In one aspect the invention relates to a method for testing a chemical entity for its capability to modulate a (poly)peptide that is malfunctioning by means of an interaction of said chemical entity and said (poly)peptide, the method using single-molecule force spectroscopy. In another aspect the invention relates to a method for testing a chemical or physical entity for its capability to interact with a G protein-coupled receptor (GPCR) in its natural membrane environment, the method using single-molecule force spectroscopy.10-08-2009
20080207466System for high throughput GPCR functional assay - A functional assay detection system for membrane bound proteins. The system comprises a biological array including a porous substrate having a plurality of membranes adhered thereto and a first side and a second side, a fluorescent labeling reagent configured to couple to the membrane bound proteins, a pulsed light assembly configured to excite the fluorescent labeling reagent, and a time-delayed imaging device configured to capture emitted fluorescence of the fluorescent labeling reagent. The pulsed light assembly is configured to excite the fluorescent labeling reagent from at least one of the first side and the second side of the porous substrate, and the fluorescent labeling reagent comprises a fluorophore that has an emission lifetime that is in the range of microseconds.08-28-2008
20080287316Screening arrangement for screening immunoassay tests and agglutination tests - A screening device for performing an immunoassay test to detect the presence of a compound in a body fluid. The device includes a holder for removably receiving a membrane to which the fluid has been applied. A light is directed to the membrane. A photodetector measures the concentration of the light reflected back from the membrane. Specifically, the concentrations of reflected light from a control zone and a test zone are measured. Signals representative of the measured light concentrations are applied to a processor. If a specified concentration of predetermined light from a control zone on the membrane is detected, the processor considers the test to be successful. In the test is successful, the processor, based upon the measured concentration of reflected light from the test zone, generates data representative of the presence of the compound.11-20-2008
20080269069Method of Performing a Microarray Assay - Disclosed is a method for performing a microarray assay on one or more sample fluid(s), said fluids comprising target biological compounds. The method comprises the step of tagging said target biological compounds with labels. The following step comprises contacting said sample fluid(s) with a substrate and detecting the presence of said labels at the surface of said substrate. The method is suitable for the simultaneous analysis, in one microarray, of one or more types of target biological compounds, in one or more sample fluid(s). To this end each of said types of biological compounds is tagged with a different label so that target biological compounds belonging to different sample fluids have different labels. Said different labels are discriminable upon detection at the surface of said substrate. Also disclosed is the use of a polymer substrate in a method for performing a microarray assay.10-30-2008
20110269644STRUCTURES FOR ENHANCED DETECTION OF FLUORESCENCE - Substrates are provided for use in the detection, identification and analysis of biologic or chemical samples that are labeled with a fluorescent label, in which the plane of maximum fluorescence is displaced from a reflective substrate surface so that the intensity maximum of the standing wave interference pattern of incident and reflected probe radiation is enhanced. The format of the substrates includes substantially planar surfaces as well as substrates with introduced variations to the substrate surface, e.g., depressions, wells, pedestals and the like, disposed in arrays or other similar structures such that one or more fluorophore-comprising objects can be attached thereto.11-03-2011
20110269643MASS SPECTROMETRIC METHODS FOR DETECTING MUTATIONS IN A TARGET NUCLEIC ACID - Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and mutations in the molecules are provided.11-03-2011
20090137423Micro-electrode array - A method of fabricating a micro-electrode array comprising the steps of coating an electrode with an insulating polymer coating by screen printing an insulating polymer onto the electrode and then curing said polymer; and sonically ablating the insulating polymer coating to produce a plurality of micro-pores.05-28-2009
20090186776Microcolumn-platform based array for high-throughput analysis - A device and methods for performing biological or chemical analysis is provided. The device includes an array of three-dimensional microcolumns projecting away from a support plate. Each microcolumn has a relatively planar, first surface remote from the support plate. An array of multiple, different biological materials may be attached to the first surface. The device, when used in combination with existent micro-titer well plates, can improve efficiency of binding assays using microarrays for high-throughput capacity.07-23-2009
20090054263Grating waveguide structure for multi-analyte determinations and the use thereof - The invention relates to variable embodiments of a grating waveguide structure which enables to determine locally resolved changes of the resonance conditions for the incoupling of an excitation light into the waveguiding layer (a) of a stratified optical waveguide by means of a grating structure (c) modulated in said layer (a) or for outcoupling of a light guided in layer (a). The inventive system comprises arrays of measurement areas produced on the grating waveguide structure having different immobilized biological or biochemical or synthetic recognition elements for simultaneously binding and determining one or more analytes, wherein said excitation light is simultaneously irradiated onto an entire array of measurement areas, and the degree of satisfaction of the resonance condition for the incoupling of light into the layer (a) towards said measurement areas is simultaneously measured. The invention also relates to an optical system comprising at least one excitation light source and at least one locally resolving detector and, optionally, positioning elements for altering the angle of incidence of the excitation light onto the inventive grating waveguide structure. The invention additionally relates to a corresponding measuring method and to the use thereof. Surprisingly, it has been found that the inventive method is well-suited as an imaging detection method with high local resolution and sensitivity.02-26-2009
20100004137Characterization of biochips containing self-assembled monolayers - The present invention relates to a method of characterizing biochips with matrix-assisted laser desorption/ionization and time of flight mass spectrometry (MALDI-TOF MS).01-07-2010
20080261828Quantum Dot Template for Fast and Simultaneous Detection of Different Infectious Agents - A method and a device for detecting the presence of a predetermined substance, in which a quantum dot is produced on a substrate. The quantum dot emits a radiation at a predetermined wavelength, and is covered with a surface layer to which the predetermined substance attaches. A deviation of the value of a parameter related to the radiation is produced when the predetermined substance attaches to the surface layer. This deviation can be detected to thereby sense the presence of the predetermined substance.10-23-2008
20080261827Detection And Analysis System For Protein Array - To provide a method for detecting a protein by immobilizing a protein on a protein array substrate at a high density with controlled orientation, irradiating the immobilized protein with ultraviolet light, visible light, or infrared light, and measuring the light not absorbed by the protein and further analyzing an interaction between the protein on the substrate and another protein and/or a compound other than proteins, a system used for the method, and to provide a protein array suitable for the system. A system including a protein array in which a protein is immobilized in aligned position on a light-transmissive substrate at a high density, a light-irradiating means, and a light-detecting means and being for detecting or analyzing a protein on the protein array and/or a compound which is other than proteins and interacts with the immobilized protein, wherein the protein array is irradiated with light by the light-irradiating means, and the light transmitted through the protein array is measured by the light-detecting means for detecting the light absorption of the protein on the protein array and/or the compound which is other than proteins and interacts with the immobilized protein.10-23-2008
20120077709Biomarkers for Differentiating Between Type 1 and Type 2 Diabetes - Biomarkers that are diagnostic of type 1 diabetes, type 2 diabetes and/or diabetic disorder are identified. Detection of different biomarkers of the invention are also diagnostic of the degree of severity of type 1 diabetes, type 2 diabetes and/or diabetic disorder. An analysis includes the parameters of matching for BMI and Tanner stage. Receiver-operator characteristic (ROC) curves were established to assess association of the biomarkers with a disease.03-29-2012
20120077708BETA-2 MICROGLOBULIN AS A BIOMARKER FOR PERIPHERAL ARTERY DISEASE - The present invention provides β2 microglobulin as a biomarker for qualifying or assessing peripheral artery disease in a subject.03-29-2012
20120077707MICROWELLS WITH MRI READABLE INDICIA - A microwell including a plurality of MRI-readable indicia. The indicia indicate the identification or location of at least one predefined well or a plurality of wells. The indicia are selected from a mark, figure, number, text, code, barcode, readable texture or indication thereof.03-29-2012
20090239767BIOMOLECULE DETECTION REAGENT AND METHOD FOR DETECTING BIOMOLECULE USING REAGENT - A biomolecule detection reagent comprising semiconductor nanoparticles and magnetic nanoparticles, incorporated in a bead comprising an inorganic compound or an organic polymer, and a surface of the beads is modified with a biomolecule detection molecule.09-24-2009
20090099038CELL LINE, SYSTEM AND METHOD FOR OPTICAL-BASED SCREENING OF ION-CHANNEL MODULATORS - A variety of applications, systems, methods and constructs are implemented for use in connection with screening of ion-channel modulators. Consistent with one such system, drug candidates are screened to identify their effects on cell membrane ion channels and pumps. The system includes screening cells having light responsive membrane ion switches, voltage-gated ion switches and fluorescence producing voltage sensors. A chemical delivery device introduces the drug candidates to be screened. An optical delivery device activates the light responsive ion switches. An optical sensor monitors fluorescence produced by the voltage sensors. A processor processes data received from the optical sensor. A memory stores the data received from the optical sensor.04-16-2009
20120196770GEL-ENCAPSULATED MICROCOLONY SCREENING - Provided herein are methods and compositions useful for detecting the production of industrially useful compounds (e.g., isoprenoids, polyketides, and fatty acids) in a cell, for example, a microbial cell genetically modified to produce one or more such compounds. In some embodiments, the methods comprise encapsulating the cell in a hydrogel particle, and detecting the compound within the hydrogel particle.08-02-2012
20120094867High-Throughput Molecular Rotor Viscometry Assay - Described are compositions and methods related to determining the rate of viscosity change in a suspension in real time. The compositions and methods have a broad range of applications, including the measurement of amylase-mediated liquefaction of a starch suspension.04-19-2012
20130123144TUNABLE LCST POLYMERS AND METHODS OF PREPARATION - Polymer compositions having the chemical structure: as well as monomer compositions for producing said polymers are described. Methods for preparing these polymers and combinatorial libraries of these polymers are also described.05-16-2013
20110059864Sequence Determination By Use Of Opposing Forces - The present teachings relate to systems, methods, and the like, for analyzing biological polymers, by use of opposing forces. Among other things, the present teachings can be used to determine sequence information, such as in genetic sequencing and genotyping applications. Various embodiments are described for efficient, high throughput sequencing of nucleic-acid molecules, such as DNA. Various embodiments are described wherein nucleic-acid sequence information is determined without the need or use of extrinsic labels. As well various embodiments of methods, systems, and the like, are described, which can provide long and accurate read lengths for low-cost nucleic acid sequencing.03-10-2011
20100227776Rapid Genotyping of SNPs - Embodiments include a universal and generic method for rapidly genotyping essentially any single nucleotide polymorphism (SNP) and or other polymorphism. Various embodiments include procedures for SNP and/or allele analysis that are easy, cheap, highly multiplexable, easily automatable, and lend themselves to high-throughput. Embodiments are broadly applicable for all applications where SNP determinations are useful.09-09-2010
20100216666METHOD OF PERFORMING A BIOLOGICAL ASSAY - The present invention relates to a method of pooling samples to be analyzed for a categorical variable, wherein the analysis involves a quantitative measurement of an analyte, said method of pooling samples comprising providing a pool of n samples wherein the amount of individual samples in the pool is such that the analytes in the samples are present in a molar ratio of x08-26-2010
20130217596Detection Device And Methods Of Use - An imaging system for exciting and measuring fluorescence on or in samples comprising fluorescent materials (e.g. fluorescent labels, dyes or pigments). In one embodiment, a device is used to detect fluorescent labels on nucleic acid. In a preferred embodiment, the device is configured such that fluorescent labels in a plurality of different DNA templates are simultaneously detected.08-22-2013
20090075837FRAMELESS MULTIPLEXED MICROARRAYS - The present invention relates to novel methods for the quantitative detection of molecules in an array. In particular, the present invention relates to methods and apparatuses for producing a frameless array. In another embodiment, the present invention relates to a composition comprising nitrocellulose that is useful of producing a frameless array. In another embodiment, the present invention relates to a method for detecting a molecular interaction. In yet another embodiment, the present invention relates to kits useful for practicing the methods and apparatuses of the present invention. The present invention provides improved methods and apparatuses for the high throughput analysis of molecular interactions and quantitative detection.03-19-2009
20100222235High throughput screening methods for fuel compositions - Methods for determining deposit formation tendencies for a plurality of fluid samples of different compositions is provided. Each sample includes fuel additive compositions containing one or more fuel additives or fuel compositions containing one or more fuels and one or more fuel additives. The methods can advantageously be optimized using combinatorial chemistry, in which a database of combinations of fuel compositions are generated. As market conditions vary and/or product requirements or customer specifications change, conditions suitable for forming desired products can be identified with little or no downtime.09-02-2010
20090111711DEVICE AND METHOD FOR HIGH THROUGHPUT SCREENING OF CRYSTALLIZATION CONDITIONS IN A VAPOR DIFFUSION ENVIRONMENT - A high-density high-throughput microplate and methods for simultaneously screening a plurality of protein crystallization solutions and for producing diffraction quality protein crystals in a vapor-diffusion environment are disclosed. The microplate has defined side-by-side paired chambers of equal size, wherein the side-by-side paired chambers have a maximum volume of about 8 μl, and wherein the paired chambers have a vapor channel, therein providing vapor exchange between the side-by-side paired chambers. The microplate further includes a membrane to seal the surface of the microplate. The microplate is adapted to receive a crystallization solution in one of the side-by-side paired chambers and a protein solution in the other of the side-by-side paired chambers, wherein the protein solution and the crystallization solution interact via a vapor diffusion process, which enables the formation of protein crystals within the chamber that contains the protein solution.04-30-2009
20130143768SYSTEM AND METHOD FOR GENERATING AND/OR SCREENING POTENTIAL METAL-ORGANIC FRAMEWORKS - A system and method for systematically generating potential metal-organic framework (MOFs) structures given an input library of building blocks is provided herein. One or more material properties of the potential MOFs are evaluated using computational simulations. A range of material properties (surface area, pore volume, pore size distribution, powder x-ray diffraction pattern, methane adsorption capability, and the like) can be estimated, and in doing so, illuminate unidentified structure-property relationships that may only have been recognized by taking a global view of MOF structures. In addition to identifying structure-property relationships, this systematic approach to identify the MOFs of interest is used to identify one or more MOFs that may be useful for high pressure methane storage.06-06-2013
20100323917TAILORED NANOPOST ARRAYS (NAPA) FOR LASER DESORPTION IONIZATION IN MASS SPECTROMETRY - The production and use of semiconducting nanopost arrays made by nanofabrication is described herein. These nanopost arrays (NAPA) provide improved laser ionization yields and controllable fragmentation with switching or modulation capabilities for mass spectrometric detection and identification of samples deposited on them.12-23-2010
20130150261BREATH ANALYSIS OF PULMONARY NODULES - The present invention provides a unique profile of volatile organic compounds as breath biomarkers for lung cancer. The present invention further provides the diagnosis, prognosis and monitoring of lung cancer or predicting the response to an anti-cancer treatment through the detection of the unique profile of volatile organic compounds indicative of lung cancer at its various stages.06-13-2013
20090088341MULTIPLEX ASSAY METHOD FOR MIXED CELL POPULATIONS - Disclosed is an in vitro assay for evaluating cellular activity, in which a culture is provided that contains one or more histologically different isolated mammalian cell-line that stably and constitutively express fluorescent proteins having a different emission spectra. The culture is assessed for cellular activity by quantifying fluorescence or detecting a pattern of fluorescence from fluorescent proteins present in the culture. In some embodiments, the cellular activity of the cell-lines is assessed by determining one or more of the growth rate, migration potential, or tubule formation potential of the cell-lines using the quantified fluorescence or pattern of fluorescence. Also disclosed are cell-lines have been stably transfected with mammalian expression plasmids that constitutively express different fluorescent proteins. Kits for performing the disclosed assays, which include the disclosed fluorescent cell-lines, are also disclosed.04-02-2009
20090088342SYSTEM, APPARATUS AND METHOD FOR APPLYING MECHANICAL FORCE TO A MATERIAL - The present invention details the design and operation of a miniaturized device array in which a range of simultaneous mechanical forces are produced by a single external pressure source. The invention is primarily embodied in a microfabricated device arrays designed to rapidly probe biological cell response to various combinations of mechanical, chemical and extra-cellular matrix parameters in a high-throughput fashion.04-02-2009
20110245106Sample Carrier and Method for Achieving Comparable Analytical Results By Aligning Test Substances on a Uniform Plane - A sample carrier such as a multi-well platform with one or more recesses, in each of which a substance to be analyzed is disposed, and an analysis method in which one or more substances, each of which is located in a recess of the sample carrier, are aligned on a uniform plane relative to the surface of the sample carrier before being analyzed.10-06-2011
20100004138Reagent for detecting biopolymer and method for detecting biopolymer - Detects the presence of binding between a sample biopolymer and a probe biopolymer and the amount of binding, without modifying the sample biopolymer in any way. The present invention provides a reagent for biopolymer detection comprising semiconductor nanoparticles on which a functional group having a positive or negative charge is exposed, and a method for biopolymer detection which detects the presence of binding between a sample biopolymer and a probe biopolymer and the amount of binding by electrostatically binding a semiconductor nanoparticle on which a functional group having a positive or negative charge is exposed to a negative or positive charge of the sample biopolymer.01-07-2010
20100056393APPARATUS AND METHOD FOR DETECTING A TARGET USING SURFACE PLASMON RESONANCE - Disclosed are substrates for detecting one or more target molecules and methods for detecting molecules using surface plasmon resonance.03-04-2010
20100056392MICROSTRUCTURE AND MICRODOMAIN MICROARRAYS, METHODS OF MAKING SAME AND USES THEREOF - Disclosed are methods for direct characterization of microdomains and/or three-dimensional microstructure arrays bearing high densities of reactive sites using Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometery (MALDI-MS) and other analytical techniques. The high site density of the arrays can provide sufficient sample of each array element and/or materials bound to each element to obtain directly using common analytical techniques such as MALDI-MS. Spatially directed synthesis of heteropolymers is done through the use of pliotolabile, electrically labile, and chemically labile protecting group(s).03-04-2010
20110177974NEURAL PROTEINS AS BIOMARKERS FOR NERVOUS SYSTEM INJURY AND OTHER NEURAL DISORDERS - The present invention identities biomarkers that are diagnostic of nerve cell injury and/or neuronal disorders. Detection of different biomarkers of the invention are also diagnostic of the degree of severity of nerve injury, the cell(s) involved in the injury, and the subcellular localization of the injury.07-21-2011
20090215646SYSTEM AND METHOD OF ANALYTE DETECTION USING DIFFERENTIAL RECEPTORS - Methods and systems for detecting the presence of analytes are described. A fluid or gas sample containing one or more analytes may pass through a particle-based sensor array. Detection and analysis techniques may be applied to determine the identity and quantity of the analytes.08-27-2009
20100062949FIBER OPTIC DEVICE - The present invention is directed to a fiber optic device consisting of a fiber bundle having multiple legs of silica fibers, using a plurality of microspheres construct to attach target cells, for the assay of cytotoxic compounds. Each leg of silica fibers consists of twenty-five or more silica fibers treated with biotin and streptavidin treated microspheres which chemically bind the microspheres to the silica fibers. Further, the present invention is directed to the unique microspheres. The microspheres have a core, preferably alginate, with an outer surface of chitosan.03-11-2010
20120202709Devices and Methods for Producing and Analyzing Microarrays - Devices and methods for producing and analyzing microarrays are disclosed. In an embodiment, a method for converting a library of beads to an array of analytes includes positioning a plurality of beads having one or more analytes bound therein on a solid support in a spatially separated manner, causing the analytes to be released from the plurality of microparticles, and localizing the released analytes in discrete spots.08-09-2012
20110039730NANOSCALE OPTOFLUIDIC DEVICES FOR MOLECULAR DETECTION - An optofluidic architecture for label free, highly parallel, detection of molecular interactions is based on the use of optically resonant devices whose resonant wavelength is shifted due to a local change in refractive index caused by a positive binding event between a surface bound molecule and its solution phase target. These devices have an extremely low limit of detection and are compatible with aqueous environments. The device combines the sensitivity (limit of detection) of nanosensor technology with the parallelity of the microarray type format.02-17-2011
20080248967Process for Evaluating a Refinery Feedstock - A process for evaluating a refinery feedstock, said process comprising: (i) providing a refinery feedstock, (ii) treating said refinery feedstock to produce an array comprising a plurality of fractions having different chemical and/or physical properties, each fraction being representative of a process stream that might be present in a refinery, and (iii) analysing each of said plurality of fractions to determine one or more chemical and/or physical properties of the fractions, said analyses being performed at least partially in parallel. In a preferred embodiment, a plurality of refinery feedstocks is evaluated, each being fractionated prior to analysis of the fractions.10-09-2008
20110251104COMPOSITIONS AND METHODS FOR DETECTING DEACETYLASE ACTIVITY - A method for the quantitative determination of the activity of enzymes in the Sirtuin family through the quantification of the acetyl-ADPr product that is formed is provided. The method described allows for a wide range of substrates, including peptides, intact proteins, or protein complexes (e.g. nucleosomes) to be used as substrates without the need for additional analytical methods to be developed.10-13-2011
20090318306SYSTEM AND METHOD FOR TEMPERATURE REFERENCING FOR MELT CURVE DATA COLLECTION - The present invention relates to systems and methods of temperature referencing for melt curve data collection. More specifically, the present invention relates to systems and methods for collecting DNA melt curve data for a DNA sample and a temperature reference material.12-24-2009
20120202708COMPLEMENT FACTOR H COPY NUMBER VARIANTS FOUND IN THE RCA LOCUS - Provided herein is a variant in the RCA locus and methods for detecting the presence, absence or amount of multiple forms of the variant.08-09-2012
20090143245MICROARRAYS FOR GENOTYPING AND METHODS OF USE - The present invention provides a microarray for detecting a genotype at a polymorphic site in a plurality of nucleic acid samples, comprising a first set of nucleic acid fragments derived from the samples and a second set of nucleic acid fragments derived from a plurality of references immobilized thereon. The invention also provides a microarray comprising a set of nucleic acid fragments immobilized on the surface of the microarray, wherein the nucleic acid fragments are derived from the samples by amplifying a region in the sample containing the polymorphism through asymmetric PCR amplification. Methods of using and making the microarrays are also provided.06-04-2009
20120122731Screening molecular libraries using microfluidic devices - Screening in a microfluidic device is mediated by a magnetic field that in some manner displaces or otherwise activates the entities of interest. Entities of interest can be identified and/or separated from one or more other components provided to the microfluidic device. Microfluidic devices may have mechanisms that apply a defined magnetic field to a region of the microfluidic device where library members pass through sequentially and/or in parallel.05-17-2012
20080255001Screening of chemical compounds purified from biological sources - A method of producing a chemical compound library comprises extracting at least one extract from at least one species of plant; processing at least one of the extract(s) to remove at least one type of chemical interference to produce a processed extract; chromatographically separating the processed extract into a plurality of chromatographic fractions, each containing an amount of chemical compounds; determining the amount of chemical compounds in at least one of the chromatographic fractions; and normalizing the chromatographic fractions in which the amounts were determined to produce normalized chromatographic fractions, each such fraction comprising from about 1 microgram to about 500 micrograms of each of from one to seven chemical compounds that were present in lower concentrations in the extract and that each have a log P of from about −1 to about 5 and a molecular weight less than about 1000 Daltons; thereby to produce a chemical compound library from at least one species of plant.10-16-2008
20110053798SYSTEM FOR MIXING FLUIDS BY COALESCENCE OF MULTIPLE EMULSIONS - System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.03-03-2011
20110028346Photonic crystal microarray device for label-free multiple analyte sensing, biosensing and diagnostic assay chips - Methods and systems for label-free multiple analyte sensing, biosensing and diagnostic assay chips consisting of an array of photonic crystal microcavities along a single photonic crystal waveguide are disclosed. The invention comprises an on-chip integrated microarray device that enables detection and identification of multiple species to be performed simultaneously using optical techniques leading to a high throughput device for chemical sensing, biosensing and medical diagnostics. Other embodiments are described and claimed.02-03-2011
20090082220Surface enhanced Raman spectroscopy (SERS) systems for the detection of bacteria and methods of use thereof - Surface-enhanced Raman spectroscopic (SERS) systems and methods for detecting biomolecules of interest, such as a bacterium or virus are provided.03-26-2009
20110136691GOLD-SILVER ALLOY NANOPARTICLE CHIP, METHOD OF FABRICATING THE SAME AND METHOD OF DETECTING MICROORGANISMS USING THE SAME - Provided are a gold-silver alloy nanoparticle chip, a method of fabricating the same and a method of detecting microorganisms using the same. The gold-silver alloy nanoparticle chip includes a hydrophilized glass substrate, a self-assembled monolayer formed on the glass substrate, and gold-silver alloy nanoparticles fixed on the self-assembled monolayer. The gold-silver alloy nanoparticle chip having such a structure enables microorganisms in a water purifier and tap water to be readily detected and enables detection efficiency to be enhanced.06-09-2011
20110136692PROTEIN MICROARRAYS FOR MASS SPECTROMETRY AND METHODS OF USE THEREFOR - The present invention relates to the use of mass spectrometry to analyze arrays. The present invention provides methods for characterizing solutions comprising one or more proteins using arrays and mass spectrometry. The arrays of the present invention are coated with porous gold and utilize hydrophobic and hydrophilic self-assembled monolayers.06-09-2011
20090048122Stabilized polypeptide compositions - The invention is based, at least in part, on the development of stabilized binding molecules that consist of or comprise a stabilized scFv and methods for making such stabilized molecules.02-19-2009
20110118145COPY NUMBER ANALYSIS OF GENETIC LOCUS - Systems and methods for analyzing copy number of a target locus, detecting a disease associated with abnormal copy number of a target gene or a carrier thereof.05-19-2011
20120172256CANTILEVERED PROBES HAVING PIEZOELECTRIC LAYER, TREATED SECTION, AND RESISTIVE HEATER, AND METHOD OF USE FOR CHEMICAL DETECTION - The invention provides a liquid cell for an atomic force microscope. The liquid cell includes a liquid cell housing with an internal cavity to contain a fluid, a plurality of conductive feedthroughs traversing the liquid cell housing between the internal cavity and a dry side of the liquid cell, a cantilevered probe coupled to the liquid cell housing, and a piezoelectric drive element disposed on the cantilevered probe. The cantilevered probe is actuated when a drive voltage is applied to the piezoelectric drive element through at least one of the conductive feedthroughs. A method of imaging an object in a liquid medium and a method of sensing a target species with the liquid cell are also disclosed.07-05-2012
20100210475MICROARRAY HAVING BRIGHT FIDUCIAL MARK AND METHOD OF OBTAINING OPTICAL DATA FROM THE MIRCOARRAY - A substrate includes; a fiducial mark disposed on the substrate, an area on the substrate on which a probe material is configured to be immobilized, the area being separated from the fiducial mark, and a probe immobilization compound disposed on the area on the substrate on which the probe material is configured to be immobilized, wherein the fiducial mark has a structure which reflects irradiated light at a greater intensity than an intensity of reflected irradiated light form the area on the substrate not corresponding to the fiducial mark.08-19-2010
20110319295MASS SPECTROMETRY ASSAY FOR eIF4E AND eIF4E REGULON ACTIVITY - Provided is a highly sensitive high throughput mass spectrometry-based quantitative assay for 4E/4E regulon pathway proteins has been developed which provides for single sample multiplexed analysis, as well as the analysis of protein phosphorylation states. It may be adapted for use as the first single sample analytical method of the 4E/4E regulon biological pathway.12-29-2011
20120046201METHOD FOR CHARACTERIZING AND/OR DETERMINING SAMPLES - Characterizing and/or determining a sample employs an array of at least two of different interacting surfaces, at least one of which comprises a non-specific interacting material non-specifically interacting said sample, at least one labelling reactant, and/or combination of the sample and at least one labelling reactant. The sample and at least one labelling reactant is introduced to interact with said interacting surfaces of said array, wherein said labelling reactant is adapted to change at least one electromagnetically readable property of at least one interacting surface of said array. Then at a predetermined time point said electromagnetically readable property of at least one of said at least two different interacting surfaces of said array is detected to obtain a fingerprint of said sample; and the sample is characterized and/or determined by comparing said fingerprint of said sample with i) at least one fingerprint of at least one corresponding sample, ii) at least one finger print of an array obtained without a sample, and/or iii) at least one fingerprint of known samples.02-23-2012
20120046200Screening Methods Involving the Detection of Short-Lived Proteins - A method is provided for screening for agents that affect protein degradation rates, the method comprising: taking a library of cells, the cells expressing a fusion protein comprising a reporter protein and a protein encoded by a sequence from a cDNA library derived from a sample of cells, the sequence from the cDNA library varying within the cell library; contacting the library of cells with a plurality of agents which may affect protein degradation rates; for each agent, selecting cells in the library which express short-lived proteins based on whether the cells have different reporter signal intensities than other cells in the library, the difference being indicative of the selected cells expressing shorter lived fusion proteins than the fusion proteins expressed by the other cells in the library; and characterizing the fusion proteins expressed by the selected cells for each agent.02-23-2012
20120004138ELECTROPHORESIS APPARATUS FOR SIMULTANEOUS LOADING OF MULTIPLE SAMPLES - The present invention includes apparatus for simultaneous loading of multiple samples for molecular separation, including a separation area with walls wherein at least one of the walls has apertures having loading sites, a gel located within the separation area, and a plurality of wells within the gel. The apertures are connected to the plurality of wells by channels structurally configured to convey samples from the apertures to the wells.01-05-2012
20120208722SURFACE ENHANCED RAMAN SPECTROSCOPY PLATFORMS AND METHODS - Surface enhanced Raman spectroscopy (SERS) platforms and methods of making and using such platforms are disclosed herein. Such platforms can be made by immobilizing a biomaterial (e.g., a carbohydrate such as a glycan) by reacting an azide-functional group attached to a surface of a solid substrate with at least one cyclooctyne (e.g., a dibenzocyclooctyne) having a biomaterial or biomaterial binding group attached thereto. In certain embodiments the immobilized biomaterial can be detected using, for example, surface enhanced Raman spectroscopy.08-16-2012
20120010105LABEL-FREE HIGH THROUGHPUT BIOMOLECULAR SCREENING SYSTEM AND METHOD - A screening system and method are described herein which provide a unique and practical solution for enabling label-free high throughput screening (HTS) to aid in the discovery of new drugs. In one embodiment, the screening system enables direct binding assays to be performed in which a biomolecular interaction of a chemical compound (drug candidate) with a biomolecule (therapeutic target) can be detected using assay volumes and concentrations that are compatible with the current practices of HTS in the pharmaceutical industry. The screening system also enables the detection of bio-chemical interactions that occur in the wells of a microplate which incorporates biosensors and surface chemistry to immobilize the therapeutic target at the surface of the biosensors. The screening system also includes fluid handling and plate handling devices to help perform automated HTS assays.01-12-2012
20090156427Bio-Sensing Nanodevice - The invention provides a bio-sensing nanodevice comprising: a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—on a support, a real time receptor-ligand binding detection method, an odorant delivery system and an odorant recognition program. The biologically-derived G-protein coupled receptor can be stabilized on nanotechnology using surfactant peptide. The said nanodevice provides a greater surface area for better precision and sensitivity to odorant detection. The invention further provides a microfluidic chip containing a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—immobilized on a support, and arranged in at least two dimensional microarray system. The invention also provides a method of delivering odorant comprising the step of manipulating the bubbles in complex microfluidic networks wherein the bubbles travel in a microfluidic channel carrying a variety of gas samples to a precise location on a chip. The invention further provides method of fabricating hOR17-4 olfactory receptor.06-18-2009
20110166040COMPOSITIONS FOR USE IN IDENTIFICATION OF STRAINS OF E. COLI O157:H7 - The present invention relates generally to strain typing of 07-07-2011
20120015847DIAGNOSIS OR PROGNOSIS OF LUNG CANCER AND COLORECTAL CANCER BASED ON EXPRESSION LEVEL OF GLUTAREDOXIN 3 - The present invention relates to a biomarker for diagnosing lung cancer and colorectal cancer containing glutaredoxin 3 as an active ingredient, and a kit for diagnosing lung cancer and colorectal cancer using the same biomarker. Glutaredoxin 3 of the present invention is over-expressed in lung cancer tissue and colorectal cancer tissue, which enables early prediction of diagnosis or prognosis of lung cancer and colorectal cancer. Thus, glutaredoxin 3 can be helpfully used as a biomarker for diagnosing lung cancer and colorectal cancer.01-19-2012
20120065104COMPOSITIONS AND METHODS FOR A MEMBRANE PROTEIN CRYSTALLIZATION SCREENING KIT - The present invention comprises compositions and methods useful as a system for efficiently determining conditions that result in the formation of crystals of membrane proteins from solutions containing a membrane protein in a purified and soluble state. The system is comprised of two primary components, a solubility screen and a crystallization screen. Each component is a set of solutions. The present invention further provides a kit comprising solutions of the invention and an instructional material for the use thereof.03-15-2012
20120108465FIBER ARRAY SENSOR - A sensor array is provided including a plurality of fibers being woven to form 3-D periodic fiber structures. A selective number of the fibers include gaseous sensing materials to detect selective gases. A plurality of spacing elements provides adequate spacing between successively arranged nano-fibers. The nano-fibers and spacing elements are arranged to form a 3-D scaffolding structure for detecting specific or combinations of gaseous analytes.05-03-2012
20120071353APPARATUS AND METHOD FOR PERFORMING NUCLEIC ACID ANALYSIS - The present invention relates to optical confinements, methods of preparing and methods of using them for analyzing molecules and/or monitoring chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost single-molecular analysis.03-22-2012
20120071352Non-labeled virus detection substrate, system, and method based on inverted multi-angular cavity arrays - The present invention discloses a non-labeled virus detection substrate, system, and method based on inverted multi-angular cavity arrays. The virus detection substrate is used together with a Raman spectrometer for virus detection. The virus detection substrate has a metal layer disposed thereon, and inverted multi-angular cavities are formed in the metal layer. The cavities are arranged in a microarray. In order to detect the target, the size of the cavities should be adjusted first. Then, a laser with an optimized wavelength is applied to induce the effect of the surface enhanced Raman scattering.03-22-2012
20110105361MICROVESSELS, MICROPARTICLES, AND METHODS OF MANUFACTURING AND USING THE SAME - A method of reading a plurality of encoded microvessels used in an assay for biological or chemical analysis. The method can include providing a plurality of encoded microvessels. The microvessels can include a respective microbody and a reservoir core configured to hold a substance in the reservoir core. The microbody can include a material that surrounds the reservoir core and facilitates detection of a characteristic of the substance within the reservoir core. Optionally, the material can be transparent so as to facilitate detection of an optical characteristic of a substance within the reservoir core. The microbody can include an identifiable code associated with the substance. The method can also include determining the corresponding codes of the microvessels.05-05-2011
20100137162Method for Rapidly Screening Microbial Hosts to Identify Certain Strains with Improved Yield and/or Quality in the Expression of Heterologous Proteins - The present invention provides an array for rapidly identifying a host cell population capable of producing heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment, or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest, including therapeutic proteins, hormones, a growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.06-03-2010
20090131274DIAGNOSTIC NANOSENSOR AND ITS USE IN MEDICINE - This invention relates generally to biosensor technology, and pertains more particularly to novel multifunctional biosensors based on ordered arrays of metallic, semiconductors and magnetic nano-islands for medical, biological, biochemical, chemical and environmental applications.05-21-2009
20090131273Correlating spectral position of chemical species on a substrate with molecular weight, structure and chemical reactivity - A system for directly printing a variety of chemicals, including very large molecules on the substrate, includes a channel of nanometric dimension movable with respect to a substrate on which printing is to occur or a substrate movable with respect to the channel. Precision contact of the end aperture, or tip, of the channel with the surface deposits the chemical on the surface. Precision contact can be made by normal force atomic force microscopy or by other techniques that allow controlled contact or near contact with the surface on which the chemical is to be written with fine precision. Multiple channels with multiple orifices may be provided. The channel is connected to a suitable separation device such as a high performance liquid chromatograph and the chemicals are delivered through a probe orifice onto a substrate. The nanometric scale of the probe allows the chemicals to be printed on the substrate at spacings of from several nanometers to hundreds of micrometers in a fashion correlated with some external signal from a device that signals the ejection of a specific chemical.05-21-2009
20120172255ES-MS OF GLYCOPEPTIDES FOR ANALYSIS OF GLYCOSYLATION - Herein is reported a method for the determination of the glycosylation of an immunoglobulin with electrospray mass spectrometry but without the need for a chromatographic purification step after the digestion of the immunoglobulin and prior to the mass spectrometric analysis.07-05-2012
20100048419Combinatorial Screening Method and Apparatus - A combinatorial screening method and system are provided. The combinatorial system and method provide rapid data generation for characterization of phase change material. The characterization data is collected through a multipoint probe card where multiple regions are characterized in a single annealing cycle.02-25-2010
20120225798METHOD FOR NON-INVASIVE PRENATAL DIAGNOSIS - The present invention is directed to methods of detecting nucleic acids in a biological sample. The method is based on a novel combination of a base extension reaction, which provides excellent analytical specificity, and a mass spectrometric analysis, which provides excellent specificity. The method can be used, for example, for diagnostic, prognostic and treatment purposes. The method allows accurate detection of nucleic acids that are present in very small amounts in a biological sample. For example, the method of the present invention is preferably used to detect fetal nucleic acid in a maternal blood sample; circulating tumor-specific nucleic acids in a blood, urine or stool sample; and donor-specific nucleic acids in transplant recipients. In another embodiment, one can detect viral, bacterial, fungal, or other foreign nucleic acids in a biological sample.09-06-2012
20120220491RETENTATE CHROMATOGRAPHY AND PROTEIN CHIP ARRAYS WITH APPLICATIONS IN BIOLOGY AND MEDICINE - Analytes in a sample are resolved by retentate chromatography in a procedure involving adsorbing the analytes on a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.08-30-2012
20120190584Chip assay having improved efficiency - The chip includes electrodes on a substrate. The electrodes include a working electrode, a reference electrode, and a counter electrode. The reference electrode is constructed so as to not have an intrinsic potential. A self-assembly monolayer is positioned on the reference electrode. The self-assembly monolayer includes spacers and active probes. The active probes are configured to have a higher affinity for a capture probe than the spacers have for the capture probe.07-26-2012
20120231973METHOD AND APPARATUS PROVIDING ANALYTICAL DEVICE AND OPERATING METHOD BASED ON SOLID STATE IMAGE SENSOR - An analytical system-on-a-chip can be used as an analytical imaging device, for example, for detecting the presence of a chemical compound. A layer of analytical material is formed on a transparent layer overlying a solid state image sensor. The analytical material can react in known ways with at least one reactant to block light or to allow light to pass through to the array. The underlying sensor array, in turn, can process the presence, absence or amount of light into a digitized signal output. The system-on-a-chip may also include software that can detect and analyze the output signals of the device.09-13-2012
20110218120ORDERED TWO- AND THREE-DIMENSIONAL STRUCTURES OF AMPHIPHILIC MOLECULES - The invention pertains, at least in part, to a method for forming an ordered structure of amphiphilic molecules, such as proteins. The method includes contacting a population of amphiphilic molecules with a interface; compressing said population laterally to an appropriate pressure, such that an ordered structure at the interface is formed. The invention also pertains to the two- and three-dimensional ordered structures that are formed using the planar membrane compression method of the invention.09-08-2011
20120094868DETECTION OF CIRCULATING TUMOR CELLS IN PERIPHERAL BLOOD WITH AN AUTOMATED SCANNING FLUORESCENCE MICROSCOPE - An automated, highly sensitive, specific and potentially quantitative detection method using an automated microscope for identifying and enumerating rare cancer cells in blood and other fluids.04-19-2012
20120088692SYSTEMS AND METHODS FOR CHARACTERIZATION OF MOLECULES - The present invention generally provides systems and methods for the detection, identification, or characterization of differences between properties or behavior of corresponding species in two or more mixtures comprised of molecules, including biomolecules and/or molecules able to interact with biomolecules, using techniques such as partitioning. The experimental conditions established as distinguishing between the mixtures of the molecules using the systems and methods of the invention can also be used, in some cases, for further fractionation and/or characterization of the biomolecules and/or other molecules, using techniques such as single-step or multiple-step extraction, and/or by liquid-liquid partition chromatography. The methods could also be used for discovering and identifying markers associated with specific diagnostics, and can be used for screening for such markers once discovered and identified during diagnostics screening.04-12-2012
20120088691HIGHLY MULTIPLEXED REAL-TIME PCR USING ENCODED MICROBEADS - Multiple probes/primers expand the capability of single-probe real-time PCR. Multiplex real-time PCR uses multiple probe-based assays, in which each assay have a specific probe labeled with a unique fluorescent dye, resulting in different observed colors for each assay. Real-time PCR instruments can discriminate between the fluorescence generated from different dyes. Different probes/primers are labeled with different dyes that each have unique emission spectra. By combining the encoded microbeads and real-time PCR amplification, it is possible to increase the multiplexity of PCR experiments to a very large number, such as 128 with 7 digit or 4,096 with 12-digit barcode. Oligonucleotide probes/primers labeled with encoded microbeads offer the ability to monitor the reaction kinetics of each probe which is tagged with barcoded beads.04-12-2012
20120329676HIGH THROUGHPUT SCREENING METHOD OF ACID-PRODUCING MICROORGANISM - A high throughput screening system and method of an acid-producing microorganism using a mixture of at least two pH indicators are provided. The method may be useful in determining a production amount of an acid, which is a final metabolite secreted by the microorganism, more accurately, rapidly and easily.12-27-2012
20120101007SILVER NANOPLATES - A sensor for detecting of an analyte in a solution phase comprises a plurality of functionalised silver nanoplates wherein a functionalising agent is directly bonded to the surfaces of the nanoplates. The nanoplates provide a detectable wavelength shift change in their local surface plasmon resonance spectrum in response to the binding of an analyte. Two or more of the nanoplates may be electromagnetically coupled.04-26-2012
20130012414NANOSTRUCTURE BASED METHODS FOR DETECTION STRUCTURE DETERMINATION SEPARATION TRANSPORT EXTRACTION AND CONTROL OF CHEMICAL AND BIOCHEMICAL MATERIAL - The invention describes methods that use nanostructures and quantum confinement to detect and manipulate chemical and biochemical molecules. To increase selectivity and sensitivity nanostructures are built to have the density of states similar to that in analyte that will be detected and operated. Using device that incorporates such nanostructures, measuring electrical and optical properties of nanostructures and analyte or charge and energy transfer between the nanostructures and analyte a rapid and sensitive continuous detection in fluids can be achieved. Detection can be further enhanced by controlling external environmental parameters and applying external fields. In addition to be detected analyte can be positioned, moved, separated, extracted, and controlled.01-10-2013
20110263455OPTICAL SYSTEM FOR MULTIPLE REACTIONS - The invention relates to optical systems and methods for measurement of samples in multiple sample vessels using an array of light sources where the light from the light sources is divided into multiple light beams, and each beam is directed to a sample vessel.10-27-2011
20080220984Method for diagnosis of physiological states by detecting patterns of volatile analytes - Provided is a non-invasive method for identification of non-physiological, physiological or diseased states based on the volatiles in gas or biogas samples from individuals. The method uses a sensor array comprising a plurality of distinct sensors which differ from other sensors by the sensing molecules or the sol-gel holding material composition. In response to a combination of volatiles, a pattern of responses is generated which can be correlated to particular non-physiological, physiological or diseased state.09-11-2008
20130184180CHARACTERIZATION OF N-GLYCANS USING EXOGLYCOSIDASES - The present disclosure provides methods for analyzing structure and/or composition of N-glycans. Such methods often involve digestion of N-glycans with multiple exoglycosidases. In some embodiments, N-glycans are digested with multiple exoglycosidases simultaneously. In some embodiments, N-glycans are digested with multiple exoglycosidases sequentially. In some embodiments, methods in accordance with the present disclosure involve comparison of cleavage products of N-glycans that have been digested with multiple exoglycosidases simultaneously to N-glycans that have been digested with multiple exoglycosidases sequentially.07-18-2013
20130184181ANALYTE DETERMINATION UTILIZING MASS TAGGING REAGENTS COMPRISING A NON-ENCODED DETECTABLE LABEL - This invention pertains to methods, mixtures, kits and compositions pertaining to analyte determination and/or quantification by mass spectrometry using compounds comprising a reporter moiety and a non-encoded detectable label. The compounds can be used in sets for the analysis of mixtures of labeled analytes.07-18-2013
20130178395Aza-Benzazolium Containing Cyanine Dyes - Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.07-11-2013
20130096030MULTISENSOR ARRAY FOR DETECTION OF ANALYTES OR MIXTURES THEREOF IN GAS OR LIQUID PHASE - The present invention relates to a multisensor array for detection of analytes in the gas phase or in the liquid phase, comprising at least two different chemo-selective compounds represented by the general formula (I) wherein the hetero atoms X04-18-2013
20130130936Method of Conducting an Assay - The invention is directed to droplet actuator devices and assay methods. The invention includes assay methods of conducting an assay comprising combining a sample with an umbelliferyl derivative, wherein the sample potentially comprises an enzyme capable of cleaving the umbelliferyl derivative and where the umbelliferyl derivative comprises an umbelliferyl core modified with one or more modifying moieties.05-23-2013
20120277119PROCESSES AND COMPOSITIONS FOR METHYLATION-BASED ENRICHMENT OF FETAL NUCLEIC ACID FROM A MATERNAL SAMPLE USEFUL FOR NON-INVASIVE PRENATAL DIAGNOSES - Provided are compositions and processes that utilize genomic regions differentially methylated between a mother and her fetus to separate, isolate or enrich fetal nucleic acid from a maternal sample. The compositions and processes described herein are useful for non-invasive prenatal diagnostics, including the detection of chromosomal aneuplodies.11-01-2012
20120283137MASS LABELS - A reactive mass label for labelling a biological molecule for detection by mass spectrometry, which label comprises a reactive functionality for labelling thiol groups or carbonyl groups. Also provided is a reactive mass label for labelling a biological molecule for detection by mass spectrometry, wherein the mass label comprises the following structure:11-08-2012
20130157896ULTRATHIN CALCINATED FILMS ON A GOLD SURFACE FOR HIGHLY EFFECTIVE LASER DESORPTION/ IONIZATION OF BIOMOLECULES - A nanoscale calcinated silicate film fabricated on a gold substrate for highly effective, matrix-free laser desorption ionization mass spectrometry (LDI-MS) analysis of biomolecules. The calcinated film is prepared by a layer-by-layer (LbL) deposition/calcination process wherein the thickness of the silicate layer and its surface properties are precisely controlled. The film exhibits outstanding efficiency in LDI-MS with extremely low background noise in the low-mass region, allowing for effective analysis of low mass weight samples and detection of large biomolecules including amino acids, peptides and proteins. Additional advantages for the calcinated film include ease of preparation and modification, high reproducibility, low cost and excellent reusability.06-20-2013
20110312536Flow cytometry for high throughput screening - The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.12-22-2011
20110312535Cartridge And Device For Analyzing Biological Samples Using Temperature-Controlled Biological Reactions - The cartridge according to the invention for analysing biological samples comprises: 12-22-2011
20110312534METHOD FOR PREDICTION OF HUMAN IRIS COLOR - A method for predicting the iris color of a human, the method comprising: 12-22-2011
20120028838Quantification of Gene Expression - The present invention relates to a method for measuring the amount of a target nucleic acid in a sample using a standard which is designed to have one base difference compared with the gene of interest or a “target nucleic acid sequence.” Use of such standard in combination with a method of “enhancing” the difference in the standard and the test nucleic acid sample using, for example, a base extension reaction carried right at the mutation site allowing amplification of the standard and target nucleic acids with the same efficiency and facilitating quantification of the target nucleic acid. Thereafter a means of quantifying the “enhanced” standard and target nucleic acid samples is used to determine the amount of the target nucleic acid. In the preferred embodiment, the quantification means is Mass Spectrometry.02-02-2012
20130210672Flow Cytometry For High Throughput Screening - The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.08-15-2013

Patent applications in class By measuring a physical property (e.g., mass, etc.)