Entries |
Document | Title | Date |
20080200341 | DEVICES AND METHODS FOR PERFORMING ARRAY BASED ASSAYS - Devices and methods for performing an array assay are provided. Embodiments of the subject array assay devices include (1) a base, (2) a cover, and (3) a clamping member for holding the cover to the base, wherein when the cover is operatively held to the base about a structure that includes an array assembly spaced-apart from a backing element, the array assembly and the backing element are deflected to substantially the same curvature. Embodiments of the subject methods include contacting a sample with a backing element and placing the backing element supported sample in contact with an array assembly to form a structure that includes the backing element and array assembly. The structure is then held together using a subject array assay device and the array substrate and the backing element are deflected to substantially the same curvature. | 08-21-2008 |
20080227651 | Cyclic voltammetry (CV) for identifying genomic sequence variations and detecting mismatch base pairs, such as single nucleotide polymorphisms - Cyclic voltammetry (CV) may be used with novel sensors for identifying the presence of target sequences complementary to probe sequences. The sensor may include an electrode layer (which is used as a working electrode in a CV system), a conductive polymer layer, and probes immobilized (e.g., via sulfur) on the conductive polymer layer. The conductive polymer layer may be polyaniline, or the like. The probes may be immobilized on the polymer layer using an electro-chemical immobilization technique in the presence of nucleophiles, such as thiol groups for example. The probes may be oligionucleotides. Thus, the sensors may be used for identifying genomic sequence variations and detecting mismatch base pairs, such as single nucleotide polymorphisms (SNPs) for example. | 09-18-2008 |
20080248960 | MICROFLUIDIC ARRAY SYSTEM FOR BIOLOGICAL, CHEMICAL, AND BIOCHEMICAL ASSESSMENTS - The system and method of the present application is a microfluidic array system for biological, chemical and biochemical assessments including a microfluidic chip for reaction assays, wherein the microfluidic chip includes a control layer and a fluidic layer, wherein the control layer is pressurized through pneumatic or hydraulic means in order to control the flow of the reagents in the fluidic layer. The system and method of the present application further includes a method of fabricating such a microfluidic chip, and further a method for operating the same. Lastly, the system and method of the present application further includes a system for operating and analyzing the microfluidic array including a pressure source, a fluidic source, a biochip reader, and a processor configured to control the same. | 10-09-2008 |
20080248961 | MATERIALS FOR SCREENING OF COMBINATORIAL LIBRARIES - The invention relates to the use of molecularly imprinted polymers as a method in which the selectivities of imprinted materials can be gainfully employed as binding matrices in the screening of combinational libraries. | 10-09-2008 |
20080261817 | Methods for Analyzing Global Regulation of Coding and Non-Coding RNA Transcripts Involving Low Molecular Weight RNAs - In some embodiments of the invention, methods are provided to interrogate the transcriptional activity relating to RNAs of small molecular weight. The methods employ hybridization of a large number of oligonucleotide probes with nucleic acid derived from RNAs of small molecular weight. | 10-23-2008 |
20080261818 | Methods of Identifying Pancreatic Cancer Cells - Methods that identify cells as pancreatic cancer cells based on assessing the expression of combinations of target molecules expressed preferentially on pancreatic cancer cells are disclosed. Combinations were initially discovered by microarray analysis and selected based upon tumor specificity, relative lack of cross-reactivity with normal tissues, and applicability as targets of multispecific ligands. The claimed methods encompass measuring the expression of three or more specific target molecules in combination and correlating positive expression of the combination with an identification of the cell as a pancreatic cancer cell. | 10-23-2008 |
20080261819 | IN VIVO PRODUCTION OF CYCLIC PEPTIDES FOR INHIBITING PROTEIN-PROTEIN INTERACTION - The present invention relates to methods and compositions utilizing inteins to generate libraries of cyclic peptides in vivo. The prevent invention also relates to methods for inhibiting protein-protein interaction. | 10-23-2008 |
20080274906 | Novel Predictors of Transplant Rejection Determined by Peripheral Blood Gene-Expression Profiling - The present invention provides methods and kits for predicting transplant rejection or tolerance. Methods for predicting the probability of cardiac allograft rejection via profiling of peripheral blood gene expression are also provided. | 11-06-2008 |
20080274907 | FLUOROGENIC PH SENSITIVE DYES AND THEIR METHOD OF USE - A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: | 11-06-2008 |
20080280771 | Protein MicroarraySystem - The present invention relates to automated methods, systems, and apparatuses for protein separation and analysis. In particular, the present invention provides an automated system for the separation, identification, and characterization of the phosphorylation status of protein samples, including the generation and analysis of protein microarrays. | 11-13-2008 |
20080287307 | MULTIWELL PLATE DEVICE - A multiwell plate device is provided having a frame, a substantially flat substrate and a multiwell structure supported by the substrate. The multiwell structure includes multiple bottomless wells formed therein. The substrate is supported by the frame and may be processed by an automated arrayer or instrument that is used to print or spot arrays in a pattern on a reaction surface of the substrate. Thereafter, the multiwell structure may be engaged with the substrate and the multiwell structure and substrate may be engaged with the frame in an upright orientation. For scanning or other analysis, the multiwell structure and substrate may be disengaged from the frame, inverted 180°, and then reengaged with the frame in the inverted orientation. | 11-20-2008 |
20080312092 | Photoinduced Signal Amplification Through Externally Sensitized Photofragmentation in Masked Photosensitizers - A method of photochemically amplifying the chemical signal associated with unmasking a photosensitizer and releasing a radical leaving group when a photochemical chain reaction is initiated by a sensitizer attached to a molecule of interest is provided. More specifically, provided is a method of photoinduced amplification comprising: providing a plurality of masked photosensitizers, each masked photosensitizer having a masking group bonded to a photosensitizer through a releasable covalent bond which disrupts the conjugation of the photosensitizer; providing a reaction photosensitizer in releasing proximity to a first masked photosensitizer; exciting the reaction photosensitizer with photoradiation, whereby the reaction photosensitizer induces release of the masking group from the first masked photosensitizer, producing a first unmasked photosensitizer which induces release of the masking group from a second masked photosensitizer in releasing proximity to the first masked photosensitizer, and so on. The release of the masking group from masked photosensitizer continues as long as masked photosensitizers are in releasing proximity to the reaction photosensitizer, or until a side reaction occurs which stops the chain propagation, or until the source of light is turned off. | 12-18-2008 |
20090005253 | Double Hybrid System Based on Gene Silencing by Transcriptional Interference - The invention relates to a novel double hybrid system and to the uses thereof. This system provides, in particular, a tool that enables the detection of the interruption of a protein-protein interaction. The developed system uses transcription interference as a mechanism for detecting a breaking up of interacting protein pairs. The developed double hybrid system can be applied to the screening of molecules enabling the detection of molecules breaking up a protein-protein interaction as well as enabling the identification of alleles lacking in the interaction of proteins involved in the protein-protein interactions. | 01-01-2009 |
20090005254 | Compartmentalized Screening by Microfluidic Control - The invention describes a method for the identification of compounds which bind to a target component of a bio-chemical system or modulate the activity of the target, comprising the steps of: a) compartmentalising the compounds into micro-capsules together with the target, such that only a subset of the repertoire is represented in multiple copies in any one microcapsule; and b) identifying the compound which binds to or modulates the activity of the target; wherein at least one step is performed under microfluidic control. The invention enables the screening of large repertoires of molecules which can serve as leads for drug development. | 01-01-2009 |
20090005255 | METHOD FOR DETECTING TARGET BIOLOGICAL MATERIAL USING DNA BARCODES - A method for detecting a target biological material using DNA barcodes is provided. The method comprises: attaching a first type of probes to the surface of magnetic particles to prepare magnetic particles for isolation wherein the first type of probes are at least partially complementary to a target biological material of interest; attaching a second type of probes to the surface of polymer particles to prepare polymer particles for analysis wherein the second type of probes are at least partially complementary to the target material but are different from the first type of probes, and attaching DNA barcodes as identification codes to the surface of the polymer particles wherein the DNA barcodes are present in an amount at least three times the amount of the second type of probes, have a predetermined sequence and are labeled with a label material; reacting the magnetic particles for isolation, the polymer particles for analysis and the target material in a hybridization reaction buffer to prepare composites, each of which consists of one magnetic particle for isolation, the target material and one polymer particle for analysis; separating the composites from unreacted reactants using a magnetic separator; heating the separated composites to denature the DNA barcodes present in the polymer particles for analysis and removing the magnetic particles for isolation and the polymer particles for analysis from the composites by centrifugation to isolate the DNA barcodes; and detecting a signal generated from the label material bound to the isolated DNA barcodes. | 01-01-2009 |
20090005256 | Analysis of Encoded Chemical Libraries - The invention provides methods and compositions for analysis of a mixture of DNA sequences. More particularly, the invention provides methods and compositions for analysis of encoded chemical libraries having encoding nucleic acid tags (e.g., encoded chemical libraries prepared by nucleic acid-mediated chemistry) through analyzing the nucleic acid templates. | 01-01-2009 |
20090011945 | Method For Making Microsensor Arrays For Detecting Analytes - The present invention provides a method and a device for high-throughput, simultaneous, and continuous detection of one or more analytes using a pin-printed chemical sensor array. Chemical sensors comprising [Ru(4,7-diphenyl-1,10-phenanthroline) | 01-08-2009 |
20090018026 | PROTEIN MARKERS FOR DIAGNOSING STOMACH CANCER AND THE DIAGNOSTIC KIT USING THEM - The present invention relates to protein markers for diagnosing stomach cancer and a diagnostic kit using the same, more precisely protein markers screened by two-dimensional gel electrophoresis and bioinformatics and a diagnostic kit using the same. The markers of the invention can be effectively used for diagnosing stomach cancer and evaluating the extent of progress of the cancer by confirming the expression levels of those marker proteins whose expressions differ in stomach cancer patients from in normal healthy people. | 01-15-2009 |
20090023590 | Trichloro Silyl Alkyl Isocyanate Molecule and Surface Modified Mineral Substrate - Molecule responding to formula X-E-SiCl | 01-22-2009 |
20090023591 | CARDIAC HORMONES FOR ASSESSING CARDIOVASCULAR RISK - The present invention relates to the use of cardiac hormones, particularly natriuretic peptides, for diagnosing the cardiovascular risk of a patient who is a candidate for administration of a cox-2-inhibiting compound, in particular an NSAID, selective cox-2 inhibitor, or steroid. More particularly, the present invention relates to the use of cardiac hormones, particularly natriuretic peptides, for diagnosing the cardiovascular risk of a patient who is a candidate for administration of a selective cox-2 inhibitor, comprising the steps of (a) measuring, preferably in vitro, the level of a cardiac hormone, (b) diagnosing the risk of the patient by comparing the measured level to known levels associated with different grades of risk in a patient. The most preferred cardiac hormone in the context of the present invention is NT-proBNP. Furthermore, the present invention relates to a method for diagnosing the risk of a patient to suffer from a cardiovascular complication as a consequence of administration of a cox-2 inhibiting compound, comprising the steps of (a) measuring the level of a cardiac hormone, (b) diagnosing the risk of the patient by comparing the measured level to known levels associated with different grades of risk in a patient. | 01-22-2009 |
20090036318 | Class II Human Histone Deacteylases, and Uses Related Thereto - The invention provides histone deacetylase class II nucleic acids and polypeptides, methods and reagents for their use, and related compounds including small molecule libraries containing class II histone deacetylase inhibitors. | 02-05-2009 |
20090048116 | GENE EXPRESSING ANALYSIS TOOL - The present invention provides a tool for analysis of expression of | 02-19-2009 |
20090054245 | Method for detecting gastric cancer by detecting VLDLR gene - It is an objective of the present invention to provide a highly stable and reproducible method for detecting gastric cancer, by finding a novel gastric cancer-related gene and detecting the inactivation of such cancer-related gene. The present invention provides a method for detecting gastric cancer, comprising detecting malignant transformation of a gastric tissue cell by detecting the inactivation of the human VLDLR gene in the gastric tissue cell. | 02-26-2009 |
20090054246 | Virus-like platform for rapid vaccine discovery - The invention is directed to virus-like particles (VLPs) of an RNA bacteriophage that (a) comprises a coat polypeptide of said phage modified by insertion of a heterologous peptide that is displayed on said VLP and (b) encapsidates said bacteriophage mRNA as well as populations of these VLPs, and their uses. The invention is further directed to VLPs that encapsidate heterologous substances, as well as populations of these VLPs and their uses. | 02-26-2009 |
20090062130 | NUCLEOSOME-BASED BIOSENSOR - A nucleosome-based biosensor can detect events of transcriptional activity. The biosensor includes a nucleosome-forming DNA that contains at least one nuclear responsive DNA sequence; a core histone octamer and at least two labels. In one version, the two labels are placed on the DNA. In another version, one of the labels is attached to the DNA, while the other to the core histone octamer. The sensor function is effected by measuring an emission signal, associated with the labels, which is sensitive to whether the nucleosome-forming DNA is or is not in nucleosomal configuration. The biosensor finds application, for example, in high-throughput screening for ligands to known and orphan nuclear receptors, respectively. | 03-05-2009 |
20090062131 | NUCLEIC ACID ARRAYS FOR DETECTING GENE EXPRESSION IN ANIMAL MODELS OF INFLAMMATORY DISEASES - The present invention provides nucleic acid arrays and methods of using the same for detecting gene expression in animal models of osteoarthritis or other inflammatory diseases. The nucleic acid arrays of the present invention comprise polynucleotide probes for genes that are differentially expressed in osteoarthritis-affected cartilage tissues as compared to non-osteoarthritic cartilage tissues. In one embodiment, a nucleic acid array of the present invention comprises a plurality of polynucleotide probe sets, each of which is capable of hybridizing under stringent or nucleic acid array hybridization conditions to a different respective tiling sequence selected from Table C, or the complement thereof. | 03-05-2009 |
20090062132 | ALTERNATIVE NUCLEIC ACID SEQUENCING METHODS - Embodiments are provided that provide for parallel sequencing of nucleic acid segments. In some embodiments, a single sequence is sequenced by at least two different sequencing techniques and the results compared, allowing for deficiencies or strengths of one technique to be complemented by the second technique. | 03-05-2009 |
20090062133 | Expression analysis of FKBP54 in the diagnosis and treatment of prostate cancer - The invention relates to compositions, kits, and methods for detecting, characterizing, preventing, and treating prostate cancer. FKBP markers are provided, wherein changes in the levels of expression of one or more of the FKBP markers is correlated with the presence of prostate cancer. | 03-05-2009 |
20090062134 | ASSAY IMAGING APPARATUS AND METHODS - A method of conducting an assay on a plurality of samples is provided. The method includes the steps of performing an assay at each sample site in a sample array having greater than 100 sample sites simultaneously illuminating each sample site using one or more LEDs, and simultaneously imaging each of the sample sites to produce imaging data pertinent to the optical effect of each site. Each assay provides an optical effect. | 03-05-2009 |
20090075827 | DIAGNOSTIC METHODS AND AGENTS - The present invention relates generally to a method and agents for profiling or stratifying an individual or group of individuals with respect to a neurological, psychiatric or psychological condition, phenotype or state, including a sub-threshold neurological, psychiatric or psychological condition, phenotype or state. More particularly, the present invention utilizes genetic means to profile or stratify individuals with respect to a neurological, psychiatric or psychological condition, phenotype or state. The present invention enables the identification of individuals at risk of these disorders thus affording the opportunity for early intervention. In addition, the subject invention allows the prediction of drug or other treatment response and adverse reactions. | 03-19-2009 |
20090075828 | INTEGRATED PROTEIN CHIP ASSAY - The present invention relates to novel methodologies for performing multiplexed assays with high precision and sensitivity. In particular, the present invention relates to improving assay sensitivity and precision by combining the normalization of multiplexed assay data using an internal standard with scattered application of samples and standards replicates throughout sample wells on a slide or set of slides as well as scattered replicates of arrayed probes in a single well. These compositions and methods can be used to perform multiplexed assays for analytes in patient and other test samples. In particular, these methods have applications for Quantitative Multi-analyte Immunoassays (QMI) to measure proteins in human serum and plasma. | 03-19-2009 |
20090075829 | Plant polymorphic markers and uses thereof - The present invention is in the field of plant genetics. More specifically, the invention relates to nucleic acid markers associated with | 03-19-2009 |
20090088328 | Microarray Quality Control - The present invention relates to methods of quality control of manufactured nucleic acid arrays. Fluorescence detection is used to evaluate the quality of a printed nucleic acid array without the need to add or otherwise link a fluorescent compound or dye to the nucleic acid. Nucleic acid arrays suitable for this method are those where the spots of the array are formed by printing a solution that contains the nucleic acid in an ion containing solution. Printing quality may be evaluated by measuring the intensity of fluorescence at the location of each printed sample, and/or by measuring the “morphology” (i.e. shape) of the printed sample. Printed spots can be “imaged” by measuring fluorescence across a spotted sample in two dimensions. The resulting image of a printed spot can be compared with a reference printed image expected for the printing equipment and solid phase used. | 04-02-2009 |
20090088329 | MULTICOMPONENT PROTEIN MICROARRAYS - The present invention involves a multicomponent protein microarray comprising two or more components of a protein-based system entrapped within spots of a biomolecule compatible matrix arranged on a surface. Also included are methods of using the microarray for multicomponent analysis along with kits and machinery comprising the microarray. | 04-02-2009 |
20090093371 | MEMBRANE ARRAYS AND METHODS OF MANUFACTURE - The invention relates to G protein-coupled receptor (GPCR) microarrays on porous substrates for structural or functional analyses of GPCRs, and methods of preparing porous substrate surfaces for receiving membranes that comprise GPCRs. In one embodiment, a GPCR microarray of the invention comprises a membrane adhered to an upper surface of a porous substrate, the membrane spanning across a plurality of pores on the porous substrate to form a plurality of cavities having sufficient geometry to permit entry of assay reagents into each cavity, thereby allowing access of assay reagents to both sides of GPCR in the membrane. | 04-09-2009 |
20090099028 | Protein binding miniature proteins and uses thereof - In certain aspects, the present invention provides miniature proteins resulted from a protein scaffold such as an avian pancreatic polypeptide that can be modified by substitution of at least one amino acid residue. In other aspects, the present invention provides diagnostic and therapeutic uses of these miniature proteins. | 04-16-2009 |
20090105082 | NON-INVASIVE MOLECULAR COLONY METHODS, KITS AND APPARATUS - Disclosed are methods permitting colonies of nucleic acids produced by amplifying them in an immobilized medium to be visualized with a homogeneous fluorescence detection system which is introduced into the medium before amplification. No post-amplification steps, such as opening the gel, blotting, hybridization, and removal of unbound substrates, dyes, or probes are needed, and the growing molecular colonies can be monitored in real time. Also disclosed are methods for quantitative in situ assays of nucleic acids contained in individual cells or in tissue fragments. The methods are conveniently executed using a disposable transparent chamber containing dry matrix of the medium and equipped by inlet and outlet ports that serve for filling the chamber with a solution comprising components of amplification and detection systems, said solution being absorbed by said matrix to swell all over the inner volume of said chamber. The invention further discloses two-dimensional arrays of intact cells or microscopic particles, and methods for preparing such arrays. | 04-23-2009 |
20090111701 | METHOD OF PREPARING A SUBSTRATE WITH A COMPOSITION INCLUDING AN ORGANOBORANE INITIATOR - A method of preparing a substrate with a composition comprising (i) an organoborane initiator and (ii) a radical curable component disposed thereon includes the step of depositing the composition onto the substrate wherein at least one of (i) the organoborane initiator and (ii) the radical curable component is deposited onto the substrate in the form of a gradient pattern. An article comprises the substrate and the gradient pattern formed on the substrate. The gradient pattern is formed from a developed composition comprising the reaction product of (i) the organoborane initiator and (ii) the radical curable component. By forming the gradient pattern on the substrate, combinatorial and high-throughput methods of generating and testing the developed composition are possible, which enable characterization of the developed composition for various physical and chemical properties. | 04-30-2009 |
20090118130 | Structure-activity relationships - The present disclosure relates to compositions and methods for screening a plurality of polypeptide variants. | 05-07-2009 |
20090118131 | Genetic comparisons between grandparents and grandchildren - Displaying a comparison of genotypic information between relatives is disclosed, including receiving an indication that a first individual is a grandparent, receiving an indication that a second individual is a grandchild of the first individual, comparing the genotypic information of the first individual and the second individual and calculating a similarity score, and displaying an indication of the similarity score graphically using colors. | 05-07-2009 |
20090124506 | Membrane computing - A method of implementation of a P-system in membrane computing comprising: placing three mutually immiscible liquids into a container, such that a first of said liquids provides a barrier between the second and third liquids; providing an initial substrate to at least one of said second and third liquids; and applying a transport rule for said first liquid to allow transport of substrate between said second and said third liquid. | 05-14-2009 |
20090131264 | METHOD FOR SELECTING STABLE PROTEINS IN NON-STANDARD PHYSICOCHEMICAL CONDITIONS - The invention relates to a method for selecting proteins stable in non-standard physicochemical conditions (temperature, pressure, pH, osmolarity, salinity, solvent, etc.) comprising the expression, in an extremophilic microorganism, of variants of the protein of interest in the form of a fusion protein with a reporter protein which is stable in extreme conditions and acts as a selection marker. | 05-21-2009 |
20090137406 | RNA Detection Method - The present invention provides an RNA detection method detecting, from a reaction system containing a target sample, a target RNA chain originated from the target sample, using a surface having on the surface thereof a polymer substance which contains a first unit having a group derived from a phosphate ester composing the hydrophilic portion of a phospholipid and a second unit having a carboxylic acid derivative group composed of an electron-attractive substitutional group bound to a carbonyl group, while being provided with at least one reaction space, the reaction space having an immobilized nucleic acid primer immobilized therein. | 05-28-2009 |
20090137407 | GENOMIC LIBRARY CONSTRUCTION - Compositions and methods for amplifying nucleic acid sequences from a single cell are provided. Compositions and methods for constructing a genomic library from a single cell are also provided. | 05-28-2009 |
20090149332 | METHODS FOR DETERMINING THE ACTIVITY OF ADAM-TS PROTEASES USING THIOPEPTOLIDES - The invention concerns the use of thiopeptolides of formula R-(Xaa) | 06-11-2009 |
20090149333 | Methods of Predicting Resistance or Sensitivity to Therapies for Cancer - The present application is directed to methods of predicting the resistance of a tumor to molecularly targeted therapy and methods of predicting sensitivity of a tumor to DNA damaging therapy. The methods include: a) determining the level of expression of plurality of genes regulated by retinoblastoma tumor supressor (RB) in the individual, and b) comparing the level of expression of the plurality of genes regulated by RB in the individual with a level of expression of the plurality of genes regulated by RB in a control. The application is also directed to an RNA expression profile for the loss of RB. | 06-11-2009 |
20090149334 | METHODS AND APPARATUS FOR DETECTING LIQUID INSIDE INDIVIDUAL CHANNELS IN A MULTI-CHANNEL PLATE - There is provided a method of measuring properties of a liquid contained in individual wells inside a multi-well array, the method comprising steps of providing capacitor electrodes in the multi-well array, the electrodes adapted to detect a capacitance value of each one of the individual wells without interference of neighboring wells, measuring a capacitance inside each one of the individual wells, and using the capacitance measurements to calculate at least one property of the liquid contained in each one of the individual wells. There is further provided an apparatus for measuring properties of a liquid contained in individual wells inside a multi-well array, and a method of controlling quality of liquid handling task that is repeated across a set of individual wells inside a multi-well array. | 06-11-2009 |
20090149335 | METHOD AND USE OF MICROARRAY TECHNOLOGY AND PROTEOGENOMIC ANALYSIS TO PREDICT EFFICACY OF HUMAN AND XENOGRAPHIC CELL, TISSUE AND ORGAN TRANSPLANT - The present invention is directed to systems and proteogenomic methods for predicting the success of the transplant of a cell, tissue, or organ by providing a means to determine the quality of the cell, tissue, or organ to be transplanted. In one embodiment, the present invention uses samples from the preservation solution to obtain phenomic fingerprints correlated with transplant pre-operative and post-operative data as a pre-operative tissue diagnostic and procedural success predictive indicator. | 06-11-2009 |
20090156413 | Method, system, apparatus and device for discovering and preparing chemical compounds for medical and other uses - Disclosed in this invention are methods, systems, databases, user-interfaces, software, media, and services useful for evaluating interactions between chemical compounds and proteins and for utilizing the information resulting from such evaluation for the purpose of discovering chemical compounds for medical and other fields. An approach termed “reverse proteomics” is disclosed. This invention generates an enormously large pool of new target proteins for drug discovery, novel methods for designing of new drugs, and a previously unthinkable pool of virtually synthesized small molecules for therapeutic uses. This invention is also applicable, for example, to discovery of substitutes for environmentally hazardous chemicals, more effective agrochemicals, and healthier food additives. | 06-18-2009 |
20090163367 | Bio Drive Apparatus, and Assay Method Using the Same - A non-optical bio-disc, a bio-disc device including the non-optical bio-disc and/or optical disc, a bio-driver apparatus in which a controller disc including a controller for the bio-disc is installed, and an assay method using the same, which are suitable for labs-on-a-chips for various diagnostic assays, nucleic acid hybridization assays, and immunoassays, are provided. The bio-driver apparatus is compatible with general optical discs, including audio, CD-ROMs, DVD players, etc. Thus, the bio-driver apparatus and the assay method offer and economical and convenient alternative to existing products. In addition, the bio-driver apparatus can be readily and easily applied in connection with a computer for remote diagnosis via the internet. | 06-25-2009 |
20090163368 | Directed evolution using proteins comprising unnatural amino acids - The invention provides methods and compositions for screening polypeptide libraries that include variants comprising unnatural amino acids. In addition, the invention provides vector packaging systems and methods for packaging a nucleic acid in a vector. Compositions of vectors produced by the methods and systems are also provided | 06-25-2009 |
20090170713 | HIGH THROUGHPUT SCREENING OF MUTAGENIZED POPULATIONS - Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation. | 07-02-2009 |
20090170714 | MULTIWELL INCUBATION APPARATUS AND METHOD OF ANALYSIS USING THE SAME - A continuous temperature-gradient incubation apparatus designed to solve the problem of moving of liquid vapor generated on the high-temperature side toward the low-temperature side to thereby cause condensation. | 07-02-2009 |
20090176651 | A H+-Gated Ion Channel - The invention relates to an isolated or recombinant Na | 07-09-2009 |
20090176652 | Methods and Oligonucleotide Designs for Insertion of Multiple Adaptors into Library Constructs - Aspects described and claimed herein provide methods to insert multiple DNA adaptors into a population of circular target DNAs at defined positions and orientations with respect to one another. The resulting multi-adaptor constructs are then used in massively-parallel nucleic acid sequencing techniques. | 07-09-2009 |
20090181852 | Assays - Assays can determine multiple analytes in a sample, such as a biological marker and a drug-related compound. | 07-16-2009 |
20090186771 | NUCLEOTIDE ANALOGS - The invention provides nucleotide analogs for use in sequencing nucleic acid molecules. | 07-23-2009 |
20090192044 | Electrode addressing method - A device for addressing an electrode array of 2 | 07-30-2009 |
20090192045 | MOLECULAR STAGING OF STAGE II AND III COLON CANCER AND PROGNOSIS - Kits and articles include reagents for conducting a seven gene assay to stage colon cancer as Stage II or Stage III colon cancer. | 07-30-2009 |
20090209431 | Non-Invasive in Vitro Method to Detect Transitional Cell Carcinoma of the Bladder - The present invention refers to a non-invasive in vitro method to detect a transitional cell carcinoma of the bladder in an individual via urine analysis, to determine the stage or severity of this cancer in an individual or to monitor the effect of treatment administered to an individual suffering from this cancer. | 08-20-2009 |
20090215634 | MICROARRAY WITH TEMPERATURE SPECIFIC CONTROLS - The present invention generally relates to microarrays and particularly to microarrays in which the optimal hybridization temperature and stringency of the probes may be easily determined by a designed set of nucleic acids. The present invention further discloses the use of a designed set of nucleic acids in a microarray to determine the optimal hybridization temperature of the nucleic acids forming said microarray and to a kit for the determination of the optimal hybridization temperature of a microarray. | 08-27-2009 |
20090215635 | LABELLING STRATEGIES FOR THE SENSITIVE DETECTION OF ANALYTES - The present invention relates to methods and reagents for detecting analytes, e.g. nucleic acids. The new methods and reagents allow a simple and sensitive detection even in complex biological samples. | 08-27-2009 |
20090215636 | DIAGNOSIS OF DISEASES AND CONDITIONS BY ANALYSIS OF HISTOPATHOLOGICALLY PROCESSED BIOLOGICAL SAMPLES USING LIQUID TISSUE PREPARATIONS - The invention provides methods for diagnosing diseases such as cancer and other conditions using biological samples. Liquid Tissue samples prepared from histopathologically prepared tissue obtained from a subject surprisingly can be used to identify and, optionally, to quantify analytes that are diagnostic of the presence of a disease, condition or syndrome in the subject. | 08-27-2009 |
20090221430 | Proteomic Methods For The Identification And Use Of Putative Biomarkers Associated With The Dysplastic State In Cervical Cells Or Other Cell Types - The invention relates to methods for detecting and identifying potential biomarkers of high-grade cervical dysplasia in an individual human subject. The invention also relates to newly discovered biomarkers, as set forth in Tables 1-4 herein, which are associated with the dysplastic state of cervical cells. It has been discovered that a differential level of expression of any of these markers or combination of these markers correlates with a dysplastic condition in a human subject, e.g., a patient. | 09-03-2009 |
20090239755 | Identification of Compounds Modifying A Cellular Response - The present invention relates to methods for identifying compounds capable of modulating a cellular response. The methods involve attaching living cells to solid supports comprising a library of test compounds. The test compounds are linked to the solid support via cleavable linkers and may thus be released from the solid supports. Solid supports comprising cells, wherein the cellular response of interest has been modulated are selected and the test compound of the solid support can then be identified. The cellular response may for example be changes in complex formation between proteins. | 09-24-2009 |
20090239756 | PREDICTORS FOR METASTASIS OF BREAST CANCER - There is provided a gene expression pattern which is clinically relevant to metastasizing breast cancer. In particular, the identity of genes that are correlated with patient survival and breast cancer recurrence are provided. The gene expression profile, whether embodied in nucleic acid expression, protein expression, or other expression formats, may be used to predict survival of subjects afflicted with breast cancer and the likelihood of breast cancer recurrence. The invention thus provides for the use of a gene expression pattern (or profile or “signature”) which correlates with (and thus able to discriminate between) patients with good or poor survival outcomes. | 09-24-2009 |
20090247416 | Method and Apparatus for Analysis of Tissue Microarrays - The present techniques include methods and systems for finding correspondences between tissue spots in tissue microarray serial sections belonging to the same recipient block. The present techniques may also be used to relate individual tissue cores to clinical information. Using either a whole slide image or the relative x-y coordinates of the tissue spots on the slide, individual tissue spots in different tissue microarrays may be linked to one another and their clinical information. | 10-01-2009 |
20090253582 | CHAMBER APPARATUS - This invention provides a system that allows a user to assemble a chamber apparatus that prohibits samples from leaking or mixing with other samples or chamber array wells, when they are inserted into an array well of the chamber apparatus. In addition, the chamber frame design allows for easy assembly and disassembly for simplified use and slide substrate scanning on conventional microarray scanners. Chamber apparatus includes a chamber frame with an upper integrated gasket and a lower integrated gasket, a substrate, and a substrate frame that positions and captures the substrate. The lower integrated gasket provides a single sealing surface between the chamber frame and the substrate. The upper integrated gasket interfaces with a chamber cover forming a compression seal that prevents sample loss due to evaporation during the hybridization process. The chamber frame and substrate frame have integrated features that allow them to align and fasten to each other by latching or snapping resulting in an optimal clamping force to produce a compression seal between the integrated lower gasket and the substrate. | 10-08-2009 |
20090258789 | METHODS OF DETERMINING THE PROGNOSIS OF AN ADENOCARCINOMA - Methods are disclosed for determining the prognosis of a subject with an adenocarcinoma in an organ, such as the lung. The methods can include quantitating expression of a plurality of cytokines of interest, such as IL-1a, IL-1b, IL-2, IL-8, IL-10, IL-12, IL-15, IFN-γ and TNF-a in the adenocarcinoma and in non-cancerous tissue in the organ. Altered expression of IL-1a, IL-1b, IL-2, IL-8, IL-10, IL-12, IL-15, IFN-γ and TNF-a in the adenocarcinoma as compared to a control and in non-cancerous tissue in the organ as compared to a control determines the prognosis for the subject. Methods for determining if a therapeutic agent is effective as an anti-cancer agent are also disclosed. | 10-15-2009 |
20090264301 | METHODS UTILIZING DIFFERENTIAL SPLICING EVENTS IN BLOOD CELLS FOR THE DETECTION OF PATHOLOGICAL EVENTS - The present invention concerns new compositions and methods for the detection of pathological events. It more specifically concerns methods for the detection in vitro of the presence of a pathology or a pathological event in a subject, comprising taking a sample of blood cells from the subject and determining, in this sample, the presence of blood cells presenting a physiological state characteristic of the pathology. The invention also concerns the tools, kits and compositions for the implementation of such methods, as well as their uses in the field of human and animal health, or in experimental research for example. | 10-22-2009 |
20090286689 | METHODS FOR ANALYZING DIFFERENTIAL GENE EXPRESSION ASSOCIATED WITH MYELOPROLIFERATIVE DISORDERS (MPD) CANCER DISEASE - The present application relates to gene analysis and, in particular, to gene expression profiling for identifying molecular signature of cancer disease, in particular the G1 phase of the cell cycle, such as myeloproliferative disorders (MPD) or breast cancer and studying cancer. | 11-19-2009 |
20090291852 | Method for Amplification of Nucleotide Sequence - A method for amplification of a nucleotide sequence characterized by performing PCR amplification using mRNA isolated from a biological sample as a template and using a first primer comprising a poly(T) sequence and an additional nucleotide sequence X thereto and a second primer comprising a poly(T) sequence and an additional nucleotide sequence Y thereto, provided that the nucleotide sequences X and Y in the first and second primers, respectively, have different sequences from each other. | 11-26-2009 |
20090291853 | METHOD OF PREDICTING RISK OF LUNG CANCER RECURRENCE, AND A COMPOSITION, KIT AND MICROARRAY FOR THE SAME - Provided is a method of predicting risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, the method including: obtaining a biological sample from a lung cancer patient; measuring an expression level of at least one marker gene from the biological sample, the marker gene being selected from the group consisting of marker genes of Table 1, 2 or 3, to obtain data for the expression level of the marker gene; and determining whether the expression level of the marker gene corresponds to an expression level of a recurrence group or an expression level of a non-recurrence group. | 11-26-2009 |
20090298700 | MONOCLONAL ANTIBODY SPECIFICALLY RECOGNIZING MODIFICATION SITE AFTER TRANSLATION OF P53 AND KIT FOR ASSAYING MODIFICATION SITE CONTAINING THE SAME - The present invention provides a monoclonal antibody recognizing modification after translation of p53 in a manner specific to a modification site, an antibody microarray comprising the antibody immobilized on a substrate, etc. Disclosed is a monoclonal antibody which reacts specifically with a peptide consisting of an amino acid sequence of at least 6 consecutive amino acids containing a predetermined amino acid residue of the amino acid sequence represented by SEQ ID NO: 1, wherein the amino acid residue is phosphorylated or acetylated, or with a peptide having one to several arbitrary amino acids added to the above peptide, but does not react with the above peptide which is not phosphorylated or acetylated. | 12-03-2009 |
20090298701 | PREDICTORS OF PATIENT RESPONSE TO TREATMENT WITH EGF RECEPTOR INHIBITORS - The present invention provides methods and compositions to facilitate determining whether an EGFR-expressing cancer in an individual is an EGFR inhibitor-responsive cancer, as well as methods for determining the likelihood that a patient having an EGFR-expressing cancer will exhibit a beneficial response to an EGFR inhibitor therapy. The methods generally involve determining a normalized expression level of a gene product that correlates with EGFR inhibitor responsiveness. | 12-03-2009 |
20090305900 | GENEMAP OF THE HUMAN GENES ASSOCIATED WITH LONGEVITY - The present invention relates to the selection of a set of SNP markers for use in genome wide association studies based on linkage disequilibrium mapping. In particular, the invention relates to the fields of pharmacogenomics, diagnostics, patient therapy and the use of genetic haplotype information to predict an individual's longevity, their protection against age-related diseases and/or their response to a particular drug or drugs. | 12-10-2009 |
20090305901 | High-Throughput Cell-Based Screening System - A (bio-)chemical assay on sensing objects ( | 12-10-2009 |
20090312189 | Method of evaluating pharmacological activity - The disclosure provides a method of evaluation pharmacological activity of a compound, such as a drug candidate, which method combines computational bioinformatics with laboratory validation. In an embodiment, the method involves the creation of a network based on correlation coefficients calculated from a data set generated by exposing the compound to a biological model and gene-against-gene clustering. The network is then validated against, for example, a standard network based on natural language searching. The validated interactions are then run through a simulation scheme to develop a hierarchy of genes that are affected by the compound and that control genes in the network. Laboratory validation is conducted on any gene interactions that could not be otherwise validated. In one embodiment, the final results are compiled into a searchable database. | 12-17-2009 |
20090318300 | Method for the detection and/or identification of a microorganism - The present invention relates generally to a method of identifying micro-organisms in a sample and in particular to the identification of prokaryotic micro-organisms. In some embodiments, the present invention relates to a method for the detection and/or identification of at least one microorganism or for the simultaneous detection and/or identification of several microorganisms in a test sample wherein the test sample is contacted with one or more probes derived from a tuf gene variable region. | 12-24-2009 |
20090318301 | DIAGNOSTIC ASSAY FOR SOURCE OF INFLAMMATION - A method of diagnosing the source of local, acute inflammation has been developed based on the discovery that white cells have different patterns of gene expression, and therefore protein markers, depending on the origin of the inflammation. These differences can be readily accessed by analysis of the white cells obtained at a site to be analyzed, for example, in the synovial fluid of a knee. The analysis, by comparison with the analysis of white cells present in known conditions, can be used to differentiate between inflammation due to bacterial infection, arthritis or gout, for example. The examples demonstrate differential gene expression in cells present in synovial fluid biopsies from patients with confirmed bacterial infection as compared to patients with aseptic loosening or patients with inflammation due to gout. | 12-24-2009 |
20090325810 | DETECTION METHOD - The present invention relates generally to an array of nucleic acid molecules, the expression profiles of which characterise the anatomical origin of a cell or population of cells within the large intestine. More particularly, the present invention relates to an array of nucleic acid molecules, the expression profiles of which characterise the proximal or distal origin of a cell or population of cells within the large intestine. The expression profiles of the present invention are useful in a range of applications including, but not limited to determining the anatomical origin of a cell or population of cells which have been derived from the large intestine. Still further, since the progression of a normal cell towards a neoplastic state is often characterised by phenotypic de-differentiation, the method of the present invention also provides a means of identifying a cellular abnormality based on the expression of an incorrect expression profile relative to that which should be expressed by the subject cells when considered in light of their anatomical location within the colon. Accordingly, this aspect of the invention provides a valuable means of identifying the existence of large intestine colon cells, these being indicative of an abnormality within the large intestine such as the onset or predisposition to the onset of a condition such as a colorectal neoplasm. | 12-31-2009 |
20090325811 | GENETIC ANALYSES PREDICTIVE OF ASTHMA - The present invention is directed to genetic sequence variations that can be used to predict whether a person will develop asthma. Disease is likely to occur if certain polymorphic forms the CCL11 gene, the CCL2 gene and the TLR7 gene are present. | 12-31-2009 |
20100009856 | Randomized dna libraries and double-stranded rna libraries, use and method of production thereof - This invention relates to DNA libraries based on plasmid or viral vectors that can express double-stranded RNA of 10-30 base pairs in length with all possible sequences, where each of the double stranded RNA is formed by a single RNA molecule in the form of hairpin, or formed by two separate RNA molecules with different 3′-overhangs. Each single member in such a DNA library encodes all components of a double stranded RNA as specified above. Such a library can be used in screening for double stranded RNA species that can induce a given phenotype without prior knowledge of their target genes. This invention further relates to a method to generate such a DNA library. | 01-14-2010 |
20100009857 | Eukaryotic signal sequences for polypeptide expression and polypeptide display libraries - The present invention generally relates to methods and compositions for expressing proteins or polypeptides in prokaryotic hosts using eukaryotic signal sequences. | 01-14-2010 |
20100009858 | EMBRYONIC STEM CELL MARKERS FOR CANCER DIAGNOSIS AND PROGNOSIS - A method of predicting the development of a cancer in a patient, comprises procuring a sample of tumour tissue from the patient, determining the expression pattern of embryonic stem cell genes in the tissue, comparing the expression pattern with the corresponding expression pattern of embryonic stem cell genes in tumour tissue of reference patients with known disease histories. Also disclosed are microarrays and DNA/RNA probes for use in the method. | 01-14-2010 |
20100016171 | GLYCAN ARRAYS ON PTFE-LIKE ALUMINUM COATED GLASS SLIDES AND RELATED METHODS - Aluminum coated glass slides provide a novel glycan array platform. Specifically, aluminum coated glass slides increase sensitivity of fluorescent based assay methods. Additionally, aluminum coated glass slides allows for mass spectroscopic analysis of carbohydrates and provide a platform for examining activity of cellulases. The unique properties of ACG slides include: 1) the metal oxide layer on the surface can be activated for grafting organic compounds such as modified oligosaccharides; 2) the surface remains electrically conductive, and the grafted oligosaccharides can be simultaneously characterized by mass spectrometry and carbohydrate-binding assay; and 3) the slides are more sensitive than transparent glass slides in binding analysis. | 01-21-2010 |
20100022400 | METHOD FOR THE QUANTITATIVE MEASUREMENT OF BIOMOLECULAR TARGETS DEPOSITED ON A BIOCHIP, AND DEVICE FOR IMPLEMENTING IT - The present invention relates in particular to a method for the quantitative measurement of biomolecular targets that have been deposited on a biochip ( | 01-28-2010 |
20100029490 | INK-JET DEVICE AND METHOD FOR PRODUCING A BIOLOGICAL ASSAY SUBSTRATE USING A PRINTING HEAD AND MEANS FOR ACCELERATED MOTION - The invention provides an ink jet device for producing a biological assay substrate. The device releases a plurality of substances onto the substrate from print heads, provided with the substances. The device further comprises means to subject the printed substrates to an accelerated motion. The accelerated motion which acts about perpendicular to the surface of the substrates acts to control penetration of the substances into the substrate. The invention also relates to a method for producing a biological assay substrate, and to a biological assay substrate obtainable by such method. | 02-04-2010 |
20100029491 | METHODS AND COMPOSITIONS FOR DIAGNOSTIC USE FOR TUMOR TREATMENT - Diagnostic markers for tumors, and their use in the diagnosis and treatment of tumors are provided. | 02-04-2010 |
20100029492 | NUCLEIC ACID CHIP FOR OBTAINING BINDING PROFILE OF SINGLE STRAND NUCLEIC ACID AND UNKNOWN BIOMOLECULE, MANUFACTURING METHOD THEREOF AND ANALYSIS METHOD OF UNKNOWN BIOMOLECULE USING NUCLEIC ACID CHIP - Disclosed are a nucleic acid chip for obtaining binding profiles between unknown biomolecules and single-stranded nucleic acids, a method for manufacturing the chip, and a method for analyzing the unknown biomolecules using the chip. The nucleic acid chip is used to analyze biological significance of the unknown biomolecule in the biospecimen. The nucleic acid chip can be manufactured by reacting a biospecimen containing an unknown biomolecule with random single-stranded nucleic acids having random base sequences to determine biomolecule-binding single stranded nucleic acids capable of binding the unknown biomolecule; and synthesizing capture single stranded nucleic acids composed of the determined biomolecule-binding single stranded nucleic acids and/or single stranded nucleic acids having base sequences complementary to those of said determined biomolecule-binding single stranded nucleic acids and affixing the capture single stranded nucleic acids on a substrate. | 02-04-2010 |
20100035758 | METHOD FOR DETECTING ORAL SQUAMOUS-CELL CARCINOMA - It is an object to be achieved by the present invention to provide a method for detecting cancer and a cell growth inhibiting agent by the identification of genes exhibiting characteristic behavior in cancers such as oral squamous-cell carcinoma. The present invention provides a method for detecting cancer, which comprises detecting at least one gene alteration existing in chromosomal region 4q35 in a specimen. | 02-11-2010 |
20100048408 | Method for study, determination or evaluation by gene expression analysis - There is provided a method for study, determination or evaluation of a disease condition due to SART stressing or a pharmacological activity, for example an analgesic activity, an autonomic imbalance-ameliorating activity or an anti-stress activity, of a test substance at the gene level. It is a method for study, determination or evaluation of a pharmacological activity of a test substance, especially an analgesic activity, an autonomic imbalance-ameliorating activity, or an anti-stress activity at the gene level by comprehensively analyzing the change in gene expression in a neural tissue after administration of a test substance in a SART-stressed animal. The method enables exploration of the substance that is effective against painful diseases, stress-related diseases, autonomic-imbalance or the like, determination and evaluation of efficacy of the substance, or analysis of a target gene of the substance | 02-25-2010 |
20100048409 | METHODS FOR IDENTIFYING OR ASSAYING FOR AGENTS THAT INCREASE BETA-CELL PROLIFERATION, INSULIN SECRETION, INSULIN SENSITIVITY, GLUCOSE TOLERANCE AND DECREASE FAT MASS - The present invention is directed to cell-based and non-cell based methods of drug screening to identify agents, or assay agents, that reduce OST-PTP or gamma-carboxylase activity or expression, or increase the level of undercarboxylated osteocalcin or adiponectin activity or expression. Such agents find use in treating or preventing a disorder related to the OST-PTP signaling pathway. Such disorders include, metabolic syndrome, glucose intolerance, type 1 or type 2 diabetes, atherosclerosis, or obesity. Such agents may be used to treat disorders characterized by decreased insulin production, deceased insulin sensitivity, and decreased glucose tolerance or increased fat mass. | 02-25-2010 |
20100048410 | Bead Sorting on a Droplet Actuator - A method of sorting beads on a droplet actuator. The method may, for example, include the following steps: (a) providing a droplet actuator comprising a substrate comprising electrodes arranged for conducting droplet operations on a substrate surface; (b) providing an assay droplet on the substrate surface, the droplet comprising two or more target-capture bead populations comprising target-capture beads comprising: (i) a capture probe bound to a target substance; and (ii) a unique bar binding element which binds to a corresponding binder; (c) using droplet operations to combine the assay droplet with a bead-capture droplet comprising one or more bead-capture beads having affinity for the binding element; (d) immobilizing the one or more bead-capture beads while conducting droplet operations to separate the bead-capture beads from unbound target-capture beads; (e) resuspending the one or more bead-capture beads in a droplet, thereby providing a droplet comprising a substantially pure substance-capture bead population; and (f) using droplet operations to conduct one or more protocol steps for an assay protocol. | 02-25-2010 |
20100056380 | COMBINATORIAL BIOMINERALIZATION ARRAYS - Systems and methods employing combinatorial arrays for revealing factors which control biomineralization processes. An understanding of such control factors may be expected to allow those of skill in the art to mimic biomineralization processes so as to allow manufacture of engineered synthetic biomineralized products, such as artificial bones. Such products would be expected to have structure and properties similar or identical to natural products (e.g., bones), and exhibit improved immunological acceptance when implanted as compared to existing synthetic engineered products. | 03-04-2010 |
20100056381 | POROUS BIOLOGICAL ASSAY SUBSTRATE AND METHOD AND DEVICE FOR PRODUCING SUCH SUBSTRATE - The invention provides a porous biological assay substrate suitable for detecting at least one analyte in a biological sample fluid. The substrate comprises one or more capture probes able to each specifically bind one target analyte. The average concentration of the capture probes in pores with a size larger than the O | 03-04-2010 |
20100056382 | DNA MILLICHIP - The present invention provides novel arrays of oligonucleotide probes immobilized on a solid support in the form of a chip (millichip), which can be used for rapid and inexpensive analysis of nucleic acids. The arrays can have a plurality of different oligonucleotide probes that can provide for whole genome gene expression analysis. The millichip can be used for analysis of both RNA and DNA. | 03-04-2010 |
20100056383 | HIGH DENSITY SELF-CONTAINED BIOLOGICAL ANALYSIS - Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include high density nucleic acid amplification and detection and immuno-PCR. | 03-04-2010 |
20100056384 | Sequence Variations in PNPLA3 Associated with Hepatic Steatosis - Disclosed are methods of identifying a genetic variant in a person determined to have or be predisposed having a fatty liver by determining whether the person has PNPLA3-I148M. Also disclosed are methods of identifying a genetic variant in a person by determining whether the person has PNPLA3-I148M; and prescribing to the person a treatment to reduce liver fat or associated inflammation. | 03-04-2010 |
20100062946 | GENOME-WIDE METHOD FOR MAPPING OF ENGAGED RNA POLYMERASES QUANTITATIVELY AND AT HIGH RESOLUTION - A method is provided for detecting genome-wide transcriptionally-engaged RNA polymerases. The method can also be used to assess status and regulation of gene promoters. The method comprises permeabilizing a cell of interest or isolating the nucleus from a cell of interest; performing a nuclear run-on (NRO) reaction with the permeabilized cell or isolated nucleus, wherein a purifiable nucleotide analog is added to the NRO reaction; optimizing the number of bases traveled by engaged polymerases for high resolution and low bias for nucleotide content of transcribed sequences by limiting a second nucleotide concentration or duration of the NRO reaction; isolating NRO-RNA from the NRO reaction; hydrolyzing the NRO-RNA isolated from the NRO reaction to optimize resolution of polymerase location; selecting hydrolyzed NRO-RNA with a solid support to obtain an enriched, purified fraction of the hydrolyzed NRO-RNA; enzymatically repairing the hydrolyzed NRO-RNA; and ligating the hydrolyzed NRO-RNA to compatible adapter oligos. | 03-11-2010 |
20100069251 | METHODS FOR PRODUCING EMBRYONIC STEM CELLS FROM PARTHENOGENETIC EMBRYOS - Means for producing embryonic stem (pES) cells which have a heterozygous genome that is matched to an individual donor are provided. In one embodiment, a means for the generation and isolation of parthenogenetic embryonic stem (pES) cells which have regions of heterozygosity that are fully matched to the oocyte donor at the MHC loci (e.g. (h-)p(MI)ES cells is provided. This is in contrast to the traditional methods of parthenogenesis that generate parthenogenetic embryonic stem (pES) cells having a substantially homozygous haploidentical set of chromosomes that are homozygous at the MHC loci. | 03-18-2010 |
20100069252 | EFFICIENT METHOD FOR PARTIAL SEQUENCING OF PEPTIDE/PROTEIN USING ACID OR BASE LABILE XANTHATES - A method and system for sequencing polypeptides utilizing acid and base labile xanthates. | 03-18-2010 |
20100075859 | SYSTEM AND METHOD FOR SOLUTION BASED MULTIPARAMETER ANALYSIS OF ANALYTES - There is described a system for multiparameter analysis of analytes. The system comprises:
| 03-25-2010 |
20100075860 | MICROASSAY WITH INTERNAL REFERENCING - Specialized microfluidic networks are utilized to deposit substances on sensor surfaces. In particular, a flow-based microfluidic printhead is used as an interface to deliver multiple analytes to a sensor for simultaneous analysis. Furthermore, internal referencing is incorporated into sensor regions for improved sensitivity. | 03-25-2010 |
20100081577 | REACTOR SYSTEMS AND METHODS - A reactor system includes a housing and a plurality of reactors at least partially contained in the housing. The reactors each have a containment structure enclosing an internal space in the reactor. The containment structure including a circumferential sidewall having opposite ends and surrounding at least a portion of said internal space. The sidewall has a thermal mass and a sidewall heater adjacent an exterior surface of the sidewall. The ratio of the thermal mass of the sidewall to a volume of the portion of the internal space that is surrounded by the sidewall is relatively low. | 04-01-2010 |
20100081578 | Exchangeable sheets pre-loaded with reagent depots for digital microfluidics - The present invention provides an exchangeable, reagent pre-loaded sheets which can be temporarily applied to an electrode array on a digital microfluidic device (DMF). The substrate facilitates virtually un-limited re-use of the DMF devices avoiding cross-contamination on the electrode array itself, as well as enabling rapid exchange of pre-loaded reagents while bridging the world-to-chip interface of DMF devices. The present invention allows for the transformation of DMF into a versatile platform for lab-on-a-chip applications. | 04-01-2010 |
20100081579 | SAMPLE DEVICE PRESERVATION - A method for preserving a sample is described. A method for preserving a sample device such as microarrays, slides and membranes is described. The preservation is achieved by applying a coating composition to a sample or sample device, and curing the coating composition. Candidate coating materials for forming the coating compositions are described. Preferably, the coating composition is an optically clear, solidifying solution. Also described are preservation kits which provide materials and instructions for the preservation of sample devices. Calibration devices are also described. | 04-01-2010 |
20100081580 | In vivo library-versus-library selection of optimized protein-protein interactions - The present invention describes a rapid and efficient in vivo library-versus-library screening strategy for identifying optimally interacting pairs of heterodimerizing polypeptides. It allows for the screening of a protein library against a second protein library, rather than against a single bait protein, and thus has numerous applications in the study of protein-protein interactions. Additionally, it allows for the application of different selection stringencies. Two leucine zipper libraries, semi-randomized at the positions adjacent to the hydrophobic core, were genetically fused to either one of two designed fragments of the enzyme murine dihydrofolate reductase (mDHFR), and cotransformed into | 04-01-2010 |
20100087325 | BIOLOGICAL SAMPLE TEMPERATURE CONTROL SYSTEM AND METHOD - The present invention provides a novel approach for controlling the temperature of biological samples on a support structure. The support structure may, for instance, be a flow cell through which a reagent fluid is allowed to flow and interact with biological samples. A thermoelectric heat exchange device, such as a Peltier device, may be used to heat or cool the biological samples on the support structure. In addition, a fluid circulating heat exchange device, such as a water heating or cooling system, may be used to heat or cool the thermoelectric heat exchange device. In general, the support structure may be located on top of the thermoelectric heat exchange device which, in turn, may be located on top of the fluid circulating heat exchange device. The thermoelectric heat exchange device and fluid circulating heat exchange device may be integrated into a holder bench which may be part of a station within an imaging processing system. The holder bench may be configured to hold multiple support structures at a time. In addition, the support structures may be configured to be evaluated and imaged using both epifluorescent and total internal reflection (TIRF) excitation techniques. | 04-08-2010 |
20100087326 | HETEROCYLIC COMPOUNDS CONTAINING THE MORPHOLINE NUCLEUS THEIR PREPARATION AND USE - Herein are described heterocyclic compounds containing the morpholine nucleus prepared from amino acids, their preparation and use for therapeutic applications. | 04-08-2010 |
20100093551 | Liquid Transfer and Filter System - A mechanically simple, small, hand held device is provided based on filtering and pressure equilibration techniques involving a unique hand-operating sequence that produces air pressure within the collection tube and the device, to enable simple and rapid extraction of blood serum or plasma or other filtrate in milliliter quantities from a collected sample. The device can also provide dilution of the serum, plasma or filtrate, capture of unwanted molecular constituents or dispensing of desired reagents. Pipette extraction of diluted or undiluted blood plasma, serum or filtrate from the device can also be achieved via a septum. The device permits all functions to be performed rapidly and with minimum danger of exposure of the operator or contamination of the sample while enabling standard evacuated collection tubes to be used. | 04-15-2010 |
20100093552 | USE AND IDENTIFICATION OF BIOMARKERS FOR GASTROINTESTINAL DISEASES - The described invention relates to the identification of biomarkers for gastrointestinal diseases and provides methods utilizing the biomarkers for in drug discovery, monitoring of treatment efficacy, and diagnostics. The invention further provides methods for identifying a therapeutic target to treat ulcerative colitis, colorectal cancer, and Crohn's disease. | 04-15-2010 |
20100105564 | Stroma Derived Predictor of Breast Cancer - The invention provides methods and compositions for use in the diagnosis and management of cancer, particularly breast cancer. The invention utilizes differential gene expression profiles in tumor associated stroma and normal stroma to compile a stroma derived prognostic predictor that classifies breast cancer patients according to clinical outcome. The application provides nucleic acids, antibodies, microarray chips and kits for use with the methods described in the application. | 04-29-2010 |
20100105565 | Evolved Orthogonal Ribosomes - There is provided a method for evolving an orthogonal rRNA molecule, comprising the steps of: providing one or more libraries of mutant orthogonal rRNA molecules and introducing the libraries into cells such that the orthogonal rRNA is incorporated into ribosomes to provide orthogonal ribosomes; providing one or more orthogonal mRNA molecules which (i) are not translated by natural ribosomes, and (ii) comprise one or more orthogonal mRNA codons; assaying the translation of the of the orthogonal mRNA and selecting the orthogonal rRNA molecules which translate the orthogonal mRNA, wherein the assay in step (c) requires translation of one or more orthogonal mRNA codons in the orthogonal mRNA; and orthogonal ribosomes incorporating such rRNA molecules. | 04-29-2010 |
20100105566 | Enzyme-Catalyzed Metal Deposition for the Enhanced Detection of Analytes of Interest - The invention is directed to enhanced methods for detecting an analyte of interest in situ, by immunoassay, or by hybridization comprising binding an enzyme-labeled conjugate molecule to an analyte of interest in the presence of a redox-inactive reductive species and a soluble metal ion. The enzyme catalyzes the conversion of the inactive reductive species to an active reducing agent, which in turn reduces the metal ion to a metal atom thereby providing an enhanced means of detecting the analyte via metal deposition. | 04-29-2010 |
20100113284 | SMALL INTERFERING RNA (SIRNA) TARGET SITE BLOCKING OLIGOS AND USES THEREOF - The present invention relates to nucleic acids designed to prevent the binding of single strand RNA in protein complexes originatig from small interfering RNA (siRNA) or small hairpin RNA (shRNA) and uses thereof. | 05-06-2010 |
20100113285 | Normalization of Data Using Controls - A method of using a standard to correct for variability in sample handling, can comprise (a) adding a template of known concentration to an assay comprising a sample; (b) preamplifying the assay; (c) amplifying the assay; (d) collecting data during the amplifying; and (e) correcting the data using a comparison of data collected from the template to data collected from the sample. | 05-06-2010 |
20100113286 | METHODS FOR DETECTION OF PREECLAMPSIA - Biomarkers associated with preeclampsia were identified. Nine proteins were identified as being differentially regulated between the control and the under 28-weeks preeclamptic samples. Similarly three proteins were identified as being differentially regulated between the control and the over 28-weeks preeclamptic samples. These 12 proteins can be used as potential biomarkers in the diagnosing and prognosing of preeclampsia. | 05-06-2010 |
20100113287 | METHODS FOR ASSESSING THE REPRESENTATION OF NUCLEIC ACID MOLECULES IN A NUCLEIC ACID LIBRARY - The invention provides methods for evaluating the representation of expected nucleic acid molecules in a test population of nucleic acid molecules. The methods each comprise the steps of: (a) hybridizing a population of sample nucleic acid molecules obtained from a test population of nucleic acid molecules to a substrate comprising a population of target nucleic acid molecules, wherein (i) each target nucleic acid molecule comprises a predetermined sequence corresponding to an expected nucleic acid molecule, and (ii) each target nucleic acid molecule is localized to a defined area of the substrate; and (b) evaluating the representation of expected nucleic acid molecules in the test population of nucleic acid molecules by analyzing the pattern of hybridization of the sample population of nucleic acid molecules to the target nucleic acid molecules. | 05-06-2010 |
20100113288 | Modular System for Performing Laboratory Protocols and Associated Methods - Systems, devices, and methods for automating laboratory protocols utilizing modular processing components to allow systems to be reconfigured for processing a wide variety of disparate laboratory protocols are provided. In one aspect, a sample processing module is provided, including a housing configured to accommodate a pre-identified sample process, a standardized temperature input capable of interfacing with a temperature controller, a standardized fluid input capable of interfacing with an input fluid controller, and a standardized agitation connector capable of interfacing with an agitator. These standardized components provide interchangeability of the module with a module having a housing configured to accommodate a different pre-identified sample process in a sample processing system. | 05-06-2010 |
20100113289 | Method and system for non-competitive copy number determination by genomic hybridization DGH - A method and system for determining a copy number of one or more nucleic acid sequences in a test sample are provided. A solid surface with a plurality of probe sets labeled with a first detectable label material bound to the solid surface is provided. The labeled probes are contacted with one or more nucleic acid molecules of the test sample, under conditions suitable for hybridization, so as to form a modified solid surface. The one or more nucleic acid molecules is/are labeled with a second detectable label material. The modified solid surface is scanned to obtain first and second data, which is then mathematically transformed so as to determine the copy number of one or more nucleic acid sequences in the test sample relative to the copy number of the one or more different nucleic acid sequences in the test sample or a reference genome. | 05-06-2010 |
20100113290 | METHODS FOR ASSESSING RNA PATTERNS - Methods and compositions for the characterizing of cancers by assessing RNA levels, such as determining an RNA pattern, are provided herein. The diagnosis, prognosis, monitoring and treatment or a cancer can be determined by detecting one or more RNAs, such as microRNAs. | 05-06-2010 |
20100120624 | ASSESSMENT OF OOCYTE COMPETENCE - Methods are provided for evaluating an oocyte for fertilization and implantation. For example, methods are provided for determining whether an oocyte expresses, or does not express, one or more of a group of markers identified as differently expressed between chromosomally normal and chromosomally abnormal oocytes. Also provided, for example, are methods for determining whether a cumulus cell expresses, or does not express, one or more of a group of markers identified as differently expressed between cumulus cells associated with chromosomally normal oocytes and cumulus cells associated with chromosomally abnormal oocytes. Methods are provided for the detection of marker expression of differentially expressed genes at the RNA level, as well as at the protein level. | 05-13-2010 |
20100120625 | METHODS FOR DETECTING MODIFICATION RESISTANT NUCLEIC ACIDS - Methods are provided for, inter alia, detecting nucleic acid molecules resistant to degradation, such as a plurality of RNA molecules bound to a ribosome, using various technologies including deep sequencing. | 05-13-2010 |
20100120626 | APPARATUS AND METHODS FOR HIGH THROUGHPUT NETWORK ELECTROPHYSIOLOGY AND CELLULAR ANALYSIS - Provided herein are apparatus and methods relating to the development of instrumentation for high throughput network electrophysiology and cellular analysis. More specifically, provided herein are multiwell microelectrode arrays (MEAs) and methods for the development of such an apparatus in an inexpensive fashion with a flexible, ANSI/SBS-compliant (American National Standards Institute/Society for Biomolecular Screening) format. Microelectrode arrays are a grid of tightly spaced microelectrodes useful for stimulating and sensing electrically active cells, networks and tissue. The techniques described herein relate to the use of microfabrication in combination with certain large-area processes that have been employed to achieve multiwell MEAs in ANSI/SBS-compliant culture well formats, which are also transparent for inverted/backside microscopy compatibility. These multiwell MEAs can be used to investigate two and three-dimensional networks of electrically active cells and tissue such as cardiac, neural, and muscular in a high throughput fashion. Also being ANSI/SBS-compliant, they are compatible with machinery and robotics developed for the pharmaceutical industry for drug screening applications. | 05-13-2010 |
20100125041 | METHODS OF TESTING A PLURALITY OF TEST SAMPLES AND SYSTEMS THEREFOR - The invention is a method and system for testing the physicochemical properties of plurality of test samples in parallel. The method includes providing a test assembly having the plurality of test samples, wherein the plurality of test samples is provided over a plurality of cells in the test assembly. A test fluid, a test object, or both are provided over the plurality of test samples to determine a physicochemical property of the plurality of test samples. The physicochemical property of the plurality of test samples is determined from the displacement of the plurality of test samples. | 05-20-2010 |
20100130367 | Methods of Quantitatively Assessing Inflammation with Biosensing Nanoparticles - The present invention includes a method of using one or more biomarkers to identify individuals with inflammatory disease using Quantum Dots conjugated to targeting moieties that will specifically bind to biomarker proteins or nucleic acids encoding the biomarker, where detection of the biomarker is associated with the inflammatory disease. | 05-27-2010 |
20100130368 | Method and system for sequencing polynucleotides - Provided herein is a method of determining a sequence of a target polynucleotide. The method can include the steps of a) providing a device including an array of relatively short polynucleotides and relatively long polynucleotides immobilised on a surface of a solid support, wherein the relatively long polynucleotides are fragments of the target polynucleotide and wherein the relatively long polynucleotides are separated by a distance of at least 10 nm, whereby parts of the relatively long polynucleotides that extend beyond the relatively short polynucleotides can be individually optically resolved; and b) determining the sequence of the target polynucleotide by detecting incorporation of nucleotides into strands complementary to the relatively long polynucleotide fragments using fluorescent labels associated with the incorporated nucleotides. Also provided is system for determining a sequence of a target polynucleotide. The system can include means for carrying out steps a) and b) of the above method. | 05-27-2010 |
20100130369 | Bead-Based Multiplexed Analytical Methods and Instrumentation - Various methods, such as a method of detecting SNPs, involving (a) introducing onto a droplet actuator a solution comprising genomic DNA, extension oligos and suspension array beads; (b) dispensing on the droplet actuator one bead per droplet; (c) cleaving DNA from the bead in each droplet; (d) amplifying the cleaved DNA; (e) detecting SNP signals and barcode signals from the amplified DNA. | 05-27-2010 |
20100130370 | FLOW CELL ARRAY AND THE UTILIZATION THEREOF FOR MULTIANALYTE DETERMINATION - A one- or two-dimensional arrangement of flow cells is provided, as part of an array of sample compartments, with at least one inlet and outlet for each sample compartment, formed by a base plate and a body, with an arrangement of spatial recesses corresponding to the (geometrical) arrangement of the sample compartments, combined with the base plate. The arrangement allows for supplying to or removing from the sample compartments, which can be arranged at a high quantity on a small base area, even very small amounts of samples or reagents. An arrangement of one or more sample compartments includes a base plate and a body combined with the base plate in such a way that one or more recesses for generation of one or more flow cells fluidically sealed against one another, each with at least one inlet and one outlet, are formed between the base plate and the body, wherein at least one outlet of each flow cell is joined with a reservoir fluidically connected with the flow cell, the reservoir being operable to receive liquid exiting the flow cell, besides methods for its manufacturing and their use. | 05-27-2010 |
20100137144 | METHOD AND MEANS FOR DETECTING AND/OR QUANTIFYING HIERARCHICAL MOLECULAR CHANGE OF A CELL IN RESPONSE TO AN EXTERNAL STIMULUS - The present invention is related to a method for detecting and/or quantifying hierarchical (regulated) molecular changes of a cell in response to any external stimulus. | 06-03-2010 |
20100137145 | MEMBRANE ARRAY AND ANALYTICAL DEVICE - A membrane array used to detect one or more analytes from a small sample of fluid with high sensitivity is provided. The membrane array can be employed in various analytical devices and is especially useful for identifying analytes from whole blood with minimal or negligible background interference. | 06-03-2010 |
20100137146 | Method of Screening Antibacterial Drug Compounds - Compounds are identified simultaneously as having antibiotic activity targeting a specific microbial protein and having no or limited toxicity against eukaryotic cells by expressing the microbial protein in eukaryotic cells by expressing the microbial protein in eukaryotic cells which then are used to screen candidate antibiotic compounds. Preferably, yeast cells such as | 06-03-2010 |
20100137147 | System, method, and product for multiple wavelength detection using single source excitation - An embodiment of a method for adjusting system gain of a biological probe array scanner for a plurality of fluorophore species is described that comprises setting an excitation beam comprising an excitation wavelength at a first power level that elicits an optimal signal to noise ratio response from a first fluorophore species; scanning a biological probe array with the excitation beam; setting the excitation beam comprising the excitation wavelength at a second power level different than the first power level that elicits the optimal signal to noise ratio response from a second fluorophore species; and scanning the biological probe array with the excitation beam. | 06-03-2010 |
20100144539 | HIGH PRESSURE PARALLEL FIXED BED REACTOR AND METHOD - The present invention discloses an apparatus and method for rapid analysis of members of a combinatorial library. The apparatus includes a plurality of reactor vessels for containing individual library members, a fluid handling system that apportions a test fluid about equally between each of the vessels and a housing for enclosing the reactor vessels, the housing defining a pressure chamber, wherein the housing is configured to sustain a pressure substantially above atmospheric pressure. This allows for simultaneous screening of library members at high pressure by providing a small pressure differential on reactor components. The disclosed apparatus is especially useful for screening library members based on their ability to catalyze the conversion of fluid reactants. | 06-10-2010 |
20100144540 | CORRELATION OF MOLECULAR MARKERS WITH CLINICAL OUTCOME IN GBM PATIENTS RADIATION TREATED WITH OR WITHOUT GEFITINIB - Interestingly, for prognosis, the significant biomarkers for Gefitinib-treated GBM patients (RTOG 0211) appeared to differ compared to historical, RT and non-Gefitinib-treated GBM patients. In Gefitinib-treated patients, those with higher levels of nuclear pAKT driven by PTEN loss, higher levels of nuclear pMAPK, and lower levels of nuclear pmTOR had significantly worse clinical outcomes. In contrast, in non-Gefitinib-treated patients, patients with PTEN-deficiency, and higher levels of EGFRvIII, total EGFR, IGFR1, NFkB and lower levels of nuclear Survivin appeared to have adverse clinical outcomes, highlighting the treatment-dependency of these biomarkers. | 06-10-2010 |
20100144541 | DNA-ARRAY-EQUIPPED CARTRIDGE, ANALYZER, AND METHOD FOR USING THE DNA-ARRAY-EQUIPPED CARTRIDGE - Rotating a cartridge body | 06-10-2010 |
20100152052 | SPECIMENS FOR MICROANALYSIS PROCESSES - The present invention relates to specimens for use in microanalysis processes. One aspect of the invention is directed toward using a mold to form specimens for a microanalysis process (e.g., including an atom probe and/or transmission electron microscope processes). Other aspects of the invention are directed towards embedding specimen material (e.g., including nanoparticles) in an embedment material to produce a specimen suitable for use in a microanalysis process. Still other aspects include combining specimen material with an embedment material to enhance a microanalysis process. Yet other embodiments of the invention are directed toward combining a specimen material with multiple embedment materials to produce specimens suitable for a microanalysis process. Further aspects of the invention are directed toward analyzing at least a portion of a specimen produced by one or more of the processes discussed above. | 06-17-2010 |
20100152053 | METHOD FOR IN VITRO MONITORING OF POSTOPERATIVE CHANGES FOLLOWING LIVER TRANSPLANTATION - The present invention relates to the use of gene expression profiles, obtained in vitro from a patient sample, for detecting any postoperative tissue incompatibility reactions following a liver transplantation, wherein in patients the gene activity of a plurality of specific genes relevant to a liver transplantation is determined in a patient sample, and the specific genes and/or gene fragments for monitoring of postoperative states are selected from the group consisting of: SEQ-ID No. 1 to SEQ-ID No. 532, these preferably being subdivided into several clusters. | 06-17-2010 |
20100160171 | Methods For Detection Of Sepsis - The present invention relates to a method for diagnosis, detection, or prognosis of sepsis and its severity. More specifically, this invention uses the presence and amount of granzyme B in platelets as a marker for sepsis. | 06-24-2010 |
20100167937 | Multiple forms of Alzheimer's disease based on differences in concentrations of protein biomarkers in blood serum - The present invention relates to identification and uses of biomarkers for neurodegenerative disease, including Alzheimer's disease, and the related diseases. More specifically, the present invention relates to the identification of protein biomarkers useful for the screening, diagnosis, and differentiation of Alzheimer's disease from Parkinson's disease, other neurodegenerative diseases, and normal controls, and in the monitoring of Alzheimer's disease severity and disease mechanisms in patients. | 07-01-2010 |
20100167938 | Nucleic acid sequencing and electronic detection - Embodiments of the present invention provide devices methods for sequencing DNA using arrays of reaction regions containing sensors to monitor changes in solutions or bound molecules contained in the reaction regions. Additional embodiments provide devices and methods for sequencing DNA using arrays of reaction regions that allow for optical monitoring of solutions in the reaction regions. Chemical amplification schemes that allow DNA to be sequenced in which multiple nucleotide addition reactions are performed to detect the incorporation of a base are disclosed. By sequencing DNA using parallel reactions contained in large arrays, DNA can be rapidly sequenced. | 07-01-2010 |
20100167939 | MULTIGENE ASSAY TO PREDICT OUTCOME IN AN INDIVIDUAL WITH GLIOBLASTOMA - The present invention concerns prognosis for glioblastoma and/or assessment of the response of an individual to therapy for glioblastoma treatment. In particular, expression analysis of two or more specific genes provided in the invention is determined to predict outcome for the individual and/or to predict if the individual will respond to therapy, such as chemoradiation, for example. In specific embodiments, a multigene set from a sample from the individual is compared to a reference set of housekeeping genes. | 07-01-2010 |
20100167940 | METHODS FOR IDENTIFYING CANCER RISK - Provided herein are tissue-specific differential methylated regions (T-DMRs) and cancer-related differential methylated regions (C-DMRs) and methods of use thereof. In one embodiment of the invention, there are provided methods of detecting a cell proliferative disorder by detecting altered methylation in one or more DMRs identified herein. In another embodiment of the invention, there are provided methods of determining clinical outcome by detecting altered methylation in one or more DMRs identified herein. | 07-01-2010 |
20100173788 | BIOMARKERS FOR BREAST CANCER - The present invention provides protein-based biomarkers and biomarker combinations that are useful in qualifying breast cancer status in a patient. In particular, the biomarkers of this invention are useful to determine metastasis-free survival of breast cancer patients. The biomarkers can be detected by SELDI mass spectrometry in serum samples fractionated by means of anion exchange chromatography. Some of the biomarkers have been identified as: Apolipoprotein A1; Apolipoprotein A2; Haptoglobin alpha 1; Transferrin; Complement C3a; and truncated forms thereof. | 07-08-2010 |
20100173789 | ISCHEMIC BIOMARKERS AND THEIR USE TO PREDICT ADVERSE NEUROLOGICAL EVENTS FROM SURGERY - Methods are provided for predicting the occurrence of adverse neurological events from surgery. Such adverse events include, for example, stroke, delirium and transient ischemic attack (TIA). The methods are based on the discovery that levels of circulating cerebral NMDA receptor peptides and antibodies can be used to identify patients who are likely to suffer from an adverse neurological event. Also provided are diagnostic procedures for practicing the predictive methods of the current invention, and interventional strategies for reducing the risk of adverse neurological events from surgery. | 07-08-2010 |
20100173790 | DETECTION OF NUCLEIC ACID SEQUENCE DIFFERENCES USING THE LIGASE DETECTION REACTION WITH ADDRESSABLE ARRAYS - The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. | 07-08-2010 |
20100179066 | Method for Assessing the Risk of a Cardiovascular Disease and for Diagnosing Dyslipidemia - The invention relates to a method for assessing the risk of a cardiovascular disease and/or for diagnosing dyslipidemia in a patient, said method comprising: a) isolating in a blood sample obtained from said patient the high density lipoprotein (HDL) fraction or a subfraction thereof; and b) measuring in said HDL fraction or subfraction thereof the concentration of one or more biomarkers selected from the group consisting of Sphingosine-1-Phosphate (S1P), sphingomyelin (SM) and Apolipoprotein A-I (apoA-1). | 07-15-2010 |
20100184607 | MONOSACCHARIDE COMPOUNDS AND METHODS THEREFOR - A monosaccharide compound of formula I as shown in the specification. Processes for the preparation of the compound of formula I and methods of screening for antibacterial or antibiotic compounds involving the compound of formula I. | 07-22-2010 |
20100184608 | REFERENCE GENES FOR THE NORMALIZATION OF GENE EXPRESSION ANALYSIS DATA - The present invention relates to reference genes, primers, and probes for the normalization of gene expression analysis data from blood samples of a patient. The invention further relates to a method for the normalization of gene expression analysis data with the aid of reference genes, primers, or probes. | 07-22-2010 |
20100190650 | TRANSCRIPTOMIC BIOMARKER OF MYOCARDITIS - Molecular signatures that function as very sensitive diagnostic biomarker for myocarditis, heart disease and disorders thereof, are identified. | 07-29-2010 |
20100190651 | METHOD OF ANALYZING PROBE NUCLEIC ACID, MICROARRAY AND KIT FOR THE SAME - Provided are a method of analyzing a sequence of a first probe nucleic acid using a substrate on which a second probe nucleic acid is immobilized, and a microarray and a kit for the same. | 07-29-2010 |
20100190652 | Biomarkers for Detection of Neonatal Sepsis in Biological Fluid - The present invention concerns the identification and detection of biological fluid biomarkers of neonatal sepsis using global proteomic approaches. | 07-29-2010 |
20100197508 | Integrated Universal Chemical Detector with Selective Diffraction Array - Integrated universal chemical detector in a micro-optical chip in which chemical/bio-sensitive micro/nano-pixels are aligned to create diffraction patterns that can be visually or instrumentally categorized in order to identify a substantial plurality of agents. By using a diffraction method to create a macroscopic diffraction image, a single small array can effectively detect hundreds or even thousands of different chemicals. The apparatus can be further automated by analyzing the diffraction patterns electronically. | 08-05-2010 |
20100197509 | Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids - A method of sorting mixtures of nucleic acid strands comprising hybridizing the strands to an array of immobilized oligonucleotides, each of which includes a constant segment adjacent to a variable segment. The constant segment of the immobilized oligonucleotides can be made complementary to the ends of strands obtained by digesting a double-stranded nucleic acid with a restriction enzyme and restoring the restriction sites, thereby permitting the sorting of strands according to their variable sequences adjacent to their constant terminal restored restriction sites. | 08-05-2010 |
20100197510 | METHODS FOR RAPID DISEASE SCREENING - Methods are provided for screening for the necessity for further diagnosis of one or more diseases or conditions in a subject, which methods are based on the discovery that abnormal levels of selected analytes in a sample fluid from a subject can be correlated with specific diseases or conditions. Further provided are criteria, and methods for the determine thereof, for selected analytes with respect to selected diseases or conditions. Thus, a variety of diseases or conditions can be screened in a rapid, cost-effective composite assay. The methods are useful for screening of newborn humans for a variety of diseases and conditions, whereby additional diagnostic procedures need only be conducted for those diseases or conditions indicated by the methods of the invention. | 08-05-2010 |
20100197511 | GENE EXPRESSION PROFILING FROM FFPE SAMPLES - Methods and compositions relating to the generation and use of gene expression data from tissue samples that have been fixed and embedded are provided. The data can electronically stored and implemented as well as used to augment diagnosis and treatment of diseases. | 08-05-2010 |
20100197512 | Multi-Sample Particle Analyzer and Method for High Throughput Screening - Embodiments of the present invention provide a system and method for analyzing a plurality of samples comprising obtaining with an autosampler a plurality of samples from a first plate having a plurality of sample wells wherein the autosampler has a plurality of probes for sampling a set of samples and wherein each probe of the plurality of probes is in communication with a separate flow cytometer via a separate conduit. The plurality of samples comprising particles is moved into a fluid flow stream for each separate conduit. Adjacent ones of the plurality of samples are separated from each other in the fluid flow stream by a separation gas, thereby forming a gas-separated fluid flow stream. The gas-separated fluid flow stream is independently guided to and through each separate flow cytometer. | 08-05-2010 |
20100210469 | MICROARRAY CHIP AND METHOD FOR DETECTION OF CHROMOSOMAL ABNORMALITY - The present invention relates to techniques to detect chromosomal columns abnormalities. More specifically, the present invention is directed to a microarray chip for detecting chromosomal abnormalities comprising one or more pooled probe sets, wherein the pooled probe set is specific to a chromosomal abnormality and all probes of each pooled probe set are immobilized together in at least one spot; a method of detecting chromosomal abnormalities using the microarray chip; a kit for diagnosing diseases associated with chromosomal abnormalities comprising the microarray chip; and a method of diagnosing a disease associated with a chromosomal abnormality by identifying the chromosomal abnormality specific to the disease using the microarray chip. The present invention relates to techniques to detect chromosomal abnormalities. More specifically, the present invention is directed to a microarray chip for detecting chromosomal abnormalities comprising one or more pooled probe sets, wherein the pooled probe set is specific to a chromosomal abnormality and all probes of each pooled probe set are immobilized together in at least one spot; a method of detecting chromosomal abnormalities using the microarray chip; a kit for diagnosing diseases associated with chromosomal abnormalities comprising the microarray chip; and a method of diagnosing a disease associated with a chromosomal abnormality by identifying the chromosomal abnormality specific to the disease using the microarray chip. | 08-19-2010 |
20100210470 | METHODS FOR DETECTING PROTEIN IN PLASMA - The present invention provides methods for detecting protein in plasma comprising contacting said plasma with a protease capable of digesting said protein into at least one detectable fragment and detecting said at least one detectable fragment using high performance liquid chromatography and mass spectroscopy. | 08-19-2010 |
20100216649 | Methods for protein interaction determination - Provided are compositions and methods for identifying pairs of interacting proteins. The pair of plasmids is adapted for use in a modified two hybrid system wherein each plasmid comprises a recombinase recognition site. The method comprises the steps of providing cDNAs encoding test polypeptides, inserting the cDNAs into the first and second plasmids, recombining the first and second plasmids to obtain recombined plasmids, isolating and digesting the recombined plasmids to obtain cDNAs encoding pairs of interacting proteins, and determining the sequence of the digested fragments to determine pairs of interacting proteins. | 08-26-2010 |
20100216650 | METHOD OF PREDICTING NUCLEIC ACID HIGHER-ORDER STRUCTURE, APPARATUS FOR PREDICTING NUCLEIC ACID HIGHER-ORDER STRUCTURE, AND PROGRAM FOR PREDICTING NUCLEIC ACID HIGHER-ORDER STRUCTURE - The object of the present invention is to provide a method of predicting a higher-order structure of a nucleic acid sequence typified by a G quartet structure, and an apparatus and a program that execute the method. The method according to the present invention relates to a method of predicting a nucleic acid higher-order structure that predicts a higher-order structure of a nucleic acid sequence, the method, including the steps of: extracting bases capable of forming a higher-order structure as a higher-order structure candidate from said nucleic acid sequence; extracting bases capable of forming a stem structure as a stem structure candidate from said nucleic acid sequence; and searching an optimal combinatorial structure based on the higher-order structure candidate and the stem structure candidate. | 08-26-2010 |
20100216651 | VIRAL POLYHEDRA COMPLEXES AND METHODS OF USE - Cypoviruses and baculoviruses are notoriously difficult to eradicate because the virus particles are embedded in micron-sized protein crystals called polyhedra. The remarkable stability of polyhedra means that like bacterial spores these insect viruses remain infectious for years in soil. Although these unique in vivo protein crystals have been extensively characterized since the early 1900s, their atomic organization remains elusive. Here we describe the 2 crystal structure of both recombinant and infectious silkworm cypovirus polyhedra determined using 5-12 micron crystals purified from insect cells. These are the smallest crystals yet used for de novo X-ray protein structure determination. It was found that polyhedra are made of trimers of the viral polyhedrin protein and contain nucleotides. Although the shape of these building blocks is reminiscent of some capsid trimers, polyhedrin has a new fold and has evolved to assemble in vivo into 3-D cubic crystals rather than icosahedral shells. The polyhedrin trimers are extensively cross-linked in polyhedra by non-covalent interactions and pack with an exquisite molecular complementarity similar to that of antigen-antibody complexes. The resulting ultra-stable and sealed crystals shield the virus particles from environmental damage. The structure suggests that polyhedra can serve as the basis for the development of robust and versatile nanoparticles for biotechnological applications such as in cell culture systems, microarrays and biopesticides. | 08-26-2010 |
20100216652 | Low Level Fluorescence Detection at the Light Microscopic Level - The present invention discloses methods of removing unwanted fluorescence from a sample by photobleaching said sample to enhance detection of proteins and fragments thereof, polynucleotides and fragments thereof, and biomolecules and fragments thereof in a sample by contacting said proteins, polynucleotides and biomolecules with a fluorescent reporter, wherein said fluorescent reported comprises a fluorescent semiconductor crystal or SCN, wherein said SCN further comprises a targeting moiety. | 08-26-2010 |
20100216653 | USE OF POLYMORPHISMS IN THE TMEM132D GENE IN THE PREDICTION AND TREATMENT OF ANXIETY DISORDERS - The present invention relates to methods, compositions, kits and reagents for the diagnosis and treatment of anxiety disorders. | 08-26-2010 |
20100222223 | METHOD FOR ASSAYING SEPSIS IN HUMANS - The present invention relates to a reliable method of prediction of sepsis in humans after a trauma, wherein the level of pancreatic stone protein/regenerating protein (PSP/reg) is determined in serum, and a high level is indicative of the development of sepsis at early stages of the disease. Furthermore a method of determination of PSP/reg levels in serum is described. | 09-02-2010 |
20100227766 | Chemical Switches for Detecting Reactive Chemical Agents - Certain embodiments of the present invention relate to the preparation of microbeads that exhibit a “turn on” fluorescence response within seconds of exposure to an analyte vapor (e.g., a chemical warfare agent or a reactive stimulant). This sensing approach is modeled after the mechanism for inhibition of acetylcholinesterase enzyme activity, and utilizes a specific and irreversible reaction between phosphonyl halides and a fluorescent indicator. The present invention also relates to a sensor and a method for sensing an analyte through detection of changes in the fluorescing properties of the inventive microbeads. | 09-09-2010 |
20100227767 | STOCHASTIC CONFINEMENT TO DETECT, MANIPULATE, AND UTILIZE MOLECULES AND ORGANISMS - Methods of detecting organisms e.g. bacteria using stochastic confinement effects with microfluidic technologies involving plugs are provided. Signal amplification methods for the detection of molecules are also disclosed. | 09-09-2010 |
20100234234 | Methods and Devices for Detecting Structural Changes in a Molecule Measuring Electrochemical Impedance - The invention relates to a method of detecting a structural change in a molecule, said molecule being attached to a surface, said surface being electrically conductive, wherein the phase of the electrochemical impedance at said surface is monitored, and wherein a change in the phase in the electrochemical impedance at said surface indicates a change in the structure of said molecule. The invention further relates to methods for making arrays having molecules such as, polypeptides attached to electrically conductive surfaces such as electrodes, and to arrays. | 09-16-2010 |
20100234235 | SYSTEM, METHOD, AND PRODUCT FOR GENERATING PATTERNED ILLUMINATION - An embodiment of a method for generating an interference pattern at a probe array is described that comprises directing light at a first waveguide and second waveguide, wherein the first and second waveguides are positioned adjacent to each other and the output from the first and second waveguides produce an interference pattern; and directing the interference pattern at the probe array. | 09-16-2010 |
20100240538 | Programmable iterated elongation: a method for manufacturing synthetic genes and combinatorial dna and protein libraries - A method for manufacturing synthetic genes and combinatorial DNA and protein libraries, termed here Divide and Conquer-DNA synthesis (D&C-DNA synthesis) method. The method can be used in a systematic and automated way to synthesize any long DNA molecule and, more generally, any combinatorial molecular library having the mathematical property of being a regular set of strings. The D&C-DNA synthesis method is an algorithm design paradigm that works by recursively breaking down a problem into two or more sub-problems of the same type. The division of long DNA sequences is done in silico. The assembly of the sequence is done in vitro. The D&C-DNA synthesis method protocol consists of a tree, in which each node represents an intermediate sequence. The internal nodes are created in elongation reactions from their daughter nodes, and the leaves are synthesized directly. After each elongation only one DNA strand passes to the next level in the tree until receiving the final product. Optionally and preferably, error correction is performed to correct any errors which may have occurred during the synthetic process. | 09-23-2010 |
20100240539 | Human Autism Susceptibility Gene Encoding PRKCB1 and Uses Thereof - The present invention discloses the identification of a human autism susceptibility gene, which can be used for the diagnosis, prevention and treatment of autism and related disorders, as well as for the screening of therapeutically active drugs. The invention more specifically discloses that the PRKCB 1 gene on chromosome 16 and certain alleles thereof are related to susceptibility to autism and represent novel targets for therapeutic intervention. The present invention relates to particular mutations in the PRKCBI gene and expression products, as well as to diagnostic tools and kits based on these mutations. The invention can be used in the diagnosis of predisposition to, detection, prevention and/or treatment of Asperger syndrome, pervasive developmental disorder, childhood disintegrative disorder, mental retardation, anxiety, depression, attention deficit hyperactivity disorders, speech delay or language impairment, epilepsy, metabolic disorder, immune disorder, bipolar disease and other psychiatric and neurological diseases. | 09-23-2010 |
20100240540 | GENOTYPIC TUMOR PROGRESSION CLASSIFIER AND PREDICTOR - Actively dividing tumors appear to progress to a life threatening condition more rapidly than slowly dividing tumors. Assessing actively dividing tumors currently involves a manual assessment of the number of mitotic cells in a histological slide prepared from the tumor and assessed by a trained pathologist. Disclosed is a method for using cumulative information from a series of expressed genes to determine tumor prognosis. This cumulative information can be used to categorize tumor samples into high mitotic states or low mitotic states using a mathematical algorithm and gene expression data derived from microarrays or quantitative-Polymerase Chain Reaction (Q-PCR) data. The specific mathematical description outlines how the algorithm assesses the most informative subset of genes from the full list of genes during the assessment of each sample. | 09-23-2010 |
20100240541 | GENETIC MARKERS FOR PROGNOSIS OF ANTIFOLATE TREATMENT EFFICACY - Methods and kits for predicting the efficacy of antifolate (e.g., methotrexate) treatment of rheumatoid arthritis by detecting polymorphisms, particularly single nucleotide polymorphisms, in adenosine pathway genes. | 09-23-2010 |
20100248972 | Enhanced Expression Method - The present invention concerns a method of producing a desired heterologous gene product wherein said heterologous gene product is expressed from a strong promoter, said method comprising expressing said gene using a mutant mRNA leader which comprises one or more mutations which enhance transcription of the gene. The invention also provides a mutant Pm mRNA leader sequence, and a vector and a library comprising the leader sequence. Methods of obtaining an mRNA mutant leader and identifying an mRNA mutant leader are encompassed, along with a vector for selection or identification of an mRNA leader mutant and a use thereof for screening. | 09-30-2010 |
20100248973 | MICROELECTRONIC SENSOR DEVICE WITH AN ARRAY OF DETECTION CELLS - The invention relates to a microelectronic sensor device with a matrix array of rows (R | 09-30-2010 |
20100261611 | ASSAY SYSTEM AND METHOD - An array of electro-actuated fluid dispensers ( | 10-14-2010 |
20100261612 | Purification and Concentration of Proteins and DNA from a Complex Sample Using Isotachophoresis and a Device to Perform the Purification - A method of simultaneously co-purifying and concentrating nucleic acid and protein targets into a single volume is described. The method includes automation of the entire sample preparation process, performed by having an analyst add a sample into a device that performs all of the steps necessary to prepare a sample for analysis. The method provides for samples are not split during the sample preparation process and where common purification methods can be used for purifying multiple analytes. | 10-14-2010 |
20100267569 | Compositions, methods and kits for the diagnosis of carriers of mutations in the BRCA1 and BRCA2 genes and early diagnosis of cancerous disorders associated with mutations in BRCA1 and BRCA2 genes - The present invention relates to diagnostic compositions methods and kits for the detection of carriers of mutations in the BRCA1 and BRCA2 genes. The detection is based on the use of detecting nucleic acids or amino acid based molecules, specific for determination of the expression of at least six marker genes of the invention, in a test sample. The invention thereby provides methods compositions and kits for the diagnosis of cancerous disorders associated with mutations in the BRCA1 and BRCA2 genes, specifically, of ovarian and breast cancer. | 10-21-2010 |
20100267570 | PS-SPCL SEARCHING APPARATUS AND METHOD USING SURFACE PLASMON RESONANCE - A Positional Scanning—Synthetic Peptide Combinatorial Library (PS-SPC) searching apparatus and method using Surface Plasmon Resonance (SPR) are provided. The method includes spotting and fixing each of a plurality of peptide pools to a top of one thin metal film, inputting specific materials to the top of the thin metal film, applying a TM-mode light to a bottom of the thin metal film and exciting SPR for the thin metal film, and detecting a TM mode reflected light reflected from the thin metal film and displaying the detected light as a two-dimensional image. | 10-21-2010 |
20100267571 | NOVEL METHOD FOR SPECIMEN PREPARATION, WHICH ENSURES PRESERVATION OF TISSUE MORPHOLOGY AND NUCLEIC ACID QUALITY - The present invention aims to develop a method for specimen preparation, which ensures the maintenance of both tissue morphology and nucleic acid quality (particularly RNA quality). The present invention further aims to prepare a specimen by this method, from which desired cells are then collected by microdissection and analyzed for gene expression. A method for specimen preparation from various frozen or unfrozen organs or tissues (excluding hard tissues) of the whole body, which comprises the following steps: 1) fixing a target organ or tissue with PFA fixative; and 2) embedding the same in paraffin by the AMeX method. | 10-21-2010 |
20100267572 | SPACE EFFICIENT POLYMER SETS - The disclosure features a collection that comprises a plurality of polymers, typically nucleic acid molecules in a compact form. The molecules include all possible sequences or at least a certain percentage of all possible sequences, of a particular length. | 10-21-2010 |
20100273661 | Methods and Kits for Predicting Treatment Response in Type II Diabetes Mellitus Patients - A method for predicting treatment response of a type II diabetes patient to rosiglitazone is provided. The method involves at least one sample from a patient having type II diabetes and analyzing biomarkers predictive of a patient who will respond to treatment with rosiglitazone. The biomarkers include, at least, interleukin-8, histidine, citrate. These biomarkers are identified in at least one classification analyses selected from the group consisting of a majority-vote based classifier and support-vector machine (SVM) classifier. Also provided is a method for predicting treatment response of a type II diabetes patient to glyburide at 8 weeks post-initiation of therapy. The method involves obtaining a sample from a type II diabetes patient who has been treated with glyburide for about 4 weeks and analyzing biomarkers predictive of a patient who will respond to treatment with glyburide at 8 weeks. The biomarkers useful in this method include, at least, sphingomyelin 23:1 and L-phenylalanine. Also provided are kits useful for the methods of the invention. | 10-28-2010 |
20100273662 | Method of preparing libraries of template polynucleotides - The present invention relates to a method for preparing a library of template polynucleotides and use thereof in methods of solid-phase nucleic acid amplification. More specifically, the invention relates to a method for preparing a library of template polynucleotides that have common sequences at their 5′ ends and at their 3′ ends. | 10-28-2010 |
20100273663 | Methods and compositions related to arrhythmogenic right ventricular cardiomyopathy (ARVC) - This disclosure provides the identification of an eight base pair deletion in the 3′-untranslated region (UTR) of the striatin gene that is linked to arrhythmogenic right ventricular cardiomyopathy (ARVC) in Boxer dogs. Also provided are methods for detecting ARVC, methods of breeding Boxer dogs to reduce the prevalence or frequency of ARVC in a population, and methods of screening for a compound useful for treatment of ARVC. | 10-28-2010 |
20100279876 | DETECTION OF NUCLEIC ACID SEQUENCE DIFFERENCES BY COMPARATIVE GENOMIC HYBRIDIZATION - The present invention provides a method of detecting nucleotide sequence differences between two nucleic acid samples. The method employs a comparative genomic hybridization (CGH) technique to analyze the sequence differences between the samples. This method permits the identification of small sequence differences (e.g., sequence divergence of 1% or less) in nucleic acid samples of high complexity (e.g., an entire genome). | 11-04-2010 |
20100279877 | Method for Detection of Melanoma - The present invention provides non-invasive methods for detecting, monitoring, staging, and diagnosing malignant melanoma in a skin sample of a subject. The methods include analyzing expression in skin sample of one or more melanoma skin markers. The melanoma skin markers include IL-1 RI, endothelin-2, ephrin-A5, IGF Binding Protein 7, HLA-A0202 heavy chain, Activin A (βA subunit), TNF RII, SPC4, and CNTF Rα. The skin sample can include nucleic acids, and can be a human skin sample from a lesion suspected of being melanoma. | 11-04-2010 |
20100279878 | Biomarkers for Septic Shock Patients - The instant invention relates generally to the use of IL-8 as a biomarker in septic shock patients as an indicator of the likelihood of survival. The instant invention further relates to the use of IL-8 as a biomarker in septic shock patients for the selection of appropriate therapies. The instant invention further relates to the use of IL-8 as a biomarker for the purposes of structuring, conducting, or evaluating clinical trials or data from clinical trials. | 11-04-2010 |
20100279879 | METHOD FOR THE ANALYSIS OF BREAST CANCER DISORDERS - Method for the analysis of breast cancer disorders, comprising determining the genomic methylation status of one or more CpG dinucleotides in a sequence selected from the group of sequences according to SEQ ID NO. 1 to 10 and/or SEQ ID NO. 50 to SEQ ID NO. 60. Optionally, additionally following steps are performed, the one or more results from the methylation status test is input into a classifier that is obtained from a Diagnostic Multi Variate Model, calculating a likelihood as to whether the sample is from a normal tissue or an breast cancer tissue and/or, calculating an associated p-value for the confidence in the prediction. | 11-04-2010 |
20100279880 | SELF-ENCODING SENSOR WITH MICROSPHERES - A microsphere-based analytic chemistry system is disclosed in which self-encoding microspheres having distinct characteristic optical response signatures to specific target analytes may be mixed together while the ability is retained to identify the sensor type and location of each sensor in a random dispersion of large numbers of such sensors in a sensor array using an optically interrogatable encoding scheme. An optical fiber bundle sensor is also disclosed in which individual microsphere sensors are disposed in microwells at a distal end of the fiber bundle and are optically coupled to discrete fibers or groups of fibers within the bundle. The identities of the individual sensors in the array are self-encoded by exposing the array to a reference analyte while illuminating the array with excitation light energy. A single sensor array may carry thousands of discrete sensing elements whose combined signal provides for substantial improvements in sensor detection limits, response times and signal-to-noise ratios. | 11-04-2010 |
20100285971 | Traceability of Cellular Cycle Anomalies Targeting Oncology and Neurodegeneration - The invention concerns a novel diagnostic and/or prognostic test for cancer. More particularly, the invention concerns the human LIV21 protein, its use as cancer diagnostic and prognostic markers. The invention also concerns methods for treating cancers and neurodegenerative diseases as well as compositions therefor. | 11-11-2010 |
20100285972 | Nanofiber surfaces for use in enhanced surface area applications - This invention provides novel nanofiber enhanced surface area substrates and structures comprising such substrates, as well as methods and uses for such substrates. | 11-11-2010 |
20100285973 | METHODS AND COMPOSITIONS FOR ASSESSMENT OF PULMONARY FUNCTION AND DISORDERS - The present invention provides methods for the assessment risk of developing asthma in smokers and non-smokers using analysis of genetic polymorphisms. The present invention also relates to the use of genetic polymorphisms in assessing a subject's risk of developing asthma. Nucleotide probes and primers, kits, and microarrays suitable for such assessment are also provided. | 11-11-2010 |
20100285974 | IDENTIFICATION OF ECTOPIC PREGNANCIES - The present invention concerns methods of identifying extra-uterine (or ectopic) pregnancies and involves the screening of samples for the presence of certain molecules now known to be markers of extra-uterine pregnancies. The present invention provides a method for identifying an ectopic pregnancy, said method comprising the steps of: (a) providing a sample from a subject; (b) identifying a level of one or more of: (i) inhibin/activin BETA b subunit gene (ii) activin B (iii) cysteine-rich secretory protein 3 (CRISP-3); and/or (iv) carboxypeptidase-B1 (CPB1) (v) SLP1 (vi) elafin (vii) prolactin in the sample, wherein the level of inhibin/activin BETA b subunit gene, activin B, CRISP-3, SLP1, elafin, prolactin and/or CPB1 is associated with, or indicative of, an ectopic pregnancy. | 11-11-2010 |
20100285975 | MICROFABRICATED DROPLET GENERATOR FOR SINGLE MOLECULE/CELL GENETIC ANALYSIS IN ENGINEERED MONODISPERSED EMULSIONS - Provided are microfluidic designs and methods for rapid generation of monodisperse nanoliter volume droplets of reagent/target (e.g., molecule or cell) mix in emulsion oil. The designs and methods enable high-throughput encapsulation of a single target (e.g., DNA/RNA molecules or cells) in controlled size droplets of reagent mix. According to various embodiments, a microfabricated, 3-valve pump is used to precisely meter the volume of reagent/target mix in each droplet and also to effectively route microparticles such as beads and cells into the device, which are encapsulated within droplets at the intersection of the reagent channel and an oil channel. The pulsatile flow profile of the microfabricated pumps provides active control over droplet generation, thereby enabling droplet formation with oils that are compatible with biological reactions but are otherwise difficult to form emulsions with. | 11-11-2010 |
20100285976 | METHOD FOR DETECTING RALSTONIA SOLANACEARUM RACE 3 BIOVAR 2 - The invention concerns a method for the detection of | 11-11-2010 |
20100285977 | DIFFERENTIATION BETWEEN BRCA1-ASSOCIATED AND SPORADIC TUMOURS - The present invention relates to methods for classifying a sample as from a BRCA1-related tumour or from a sporadic tumour based on copy number analysis of a numbers of genomic locations. The invention further provides sets of nucleic acid probes, arrays and kits comprising these sets and arrays, for use in the diagnostic and/or prognostic methods of the invention for tumour classification. | 11-11-2010 |
20100285978 | Method and Device for Affinity Differential Intraplexing - The present invention provides a method and device for the improvement of intraplexed assays. This improvement is based upon the use of multiple assay chemistries having different affinity constants (K | 11-11-2010 |
20100292085 | Methods and compositions for characterization of HSD1 inhibitors - One aspect of the present invention relates to methods of identifying cortisone response signature gene sets and methods of using the identified gene sets to identify compounds that modulate HSD1 activity. In some embodiments, methods are provided to use cortisone response gene sets to estimate the HSD1 activity. Another aspect of the present inventive relates to methods for identification of off-target signature gene sets that can be used to estimate HSD1 compound induced off-target activity and methods for classification of compounds that modulate HSD1 activity. Another aspect of the present invention relates to cell lines that over-expresses HSD1 and methods of use thereof. Additional embodiments of the of the invention are described in the specification provided herein. The contents of this ABSTRACT are not intended to in anyway limit the scope of the invention claimed herein. | 11-18-2010 |
20100292086 | METHOD OF SCREENING FOR METABOLITE TRANSPORT MODIFIERS - Methods for discovering compounds that modulate metabolite transport across cell membranes are disclosed. Fluorescently labeled protein probes which have an affinity for an unbound metabolite are trapped in cells. The influx of the unbound metabolite into the cell causes a change in the fluorescence of the protein probe. The ability of test compounds to modulate the fluorescent signal is used to screen for compounds which affect transport of the unbound metabolite. In an example, the unbound metabolite is an unbound free fatty acid and the probe is a fatty acid binding protein. | 11-18-2010 |
20100304978 | Methods and compositions for identifying a fetal cell - The present invention provides methods and compositions for specifically identifying a fetal cell. An initial screening of approximately 400 candidate genes by digital PCR in different fetal and adult tissues identified a subset of 24 gene markers specific for fetal nucleated RBC and trophoblasts. The specific expression of those genes was further evaluated and verified in more defined tissues and isolated cells through quantitative RT-PCR using custom Taqman probes specific for each gene. A subset of fetal cell specific markers (FCM) was tested and validated by RNA fluorescent in situ hybridization (FISH) in blood samples from non-pregnant women, and pre-termination and post-termination pregnant women. Applications of these gene markers include, but are not limited to, distinguishing a fetal cell from a maternal cell for fetal cell identification and genetic diagnosis, identifying circulating fetal cell types in maternal blood, purifying or enriching one or more fetal cells, and enumerating one or more fetal cells during fetal cell enrichment. | 12-02-2010 |
20100304979 | Diagnostic Methods and Arrays for Use in the Same - The present invention provides a method for determining the presence of breast cancer cells in an individual comprising the steps of (a) providing a serum or plasma sample to be tested and (b) determining a protein signature of the test sample by measuring the presence and/or amount in the test sample of two or more proteins selected from the group defined in Table 1a or 1b, wherein the presence and/or amount in the test sample of the two or more proteins selected from the group defined in Table 1a or 1b is indicative of the presence of breast cancer cells. In a preferred embodiment, the two or more proteins include IL-5 and/or MCP-3. Also provided are arrays and diagnostic kits for use in the methods of the invention. | 12-02-2010 |
20100304980 | PLANAR LIPID BILAYER ARRAY FORMED BY MICROFLUIDIC TECHNIQUE AND METHOD OF ANALYSIS USING PLANAR LIPID BILAYER - There is provided a planar lipid bilayer array formed by microfluidic technique and a method of analysis using the planar lipid bilayers, providing the advantages such as portability, decreased analysis time, a smaller amount of required reagents, and parallel automation with high reproducibility. The planar lipid bilayer array formed by microfluidic technique is a planar lipid bilayer array formed by microfluidic technique (PDMS device) | 12-02-2010 |
20100304981 | BIOMARKERS FOR SEPSIS - Biomarkers for sepsis and resulting mortality can be detected by assaying blood samples. Changes in the concentration of the biomarkers can be used to indicate sepsis, risk of sepsis, progression of sepsis, remission from sepsis, and risk of mortality. Changes can be evaluated relative to data sets, natural or synthetic or semisynthetic control samples, or patient samples collected at different time points. Some biomarkers' concentrations are elevated during disease and some are depressed. These are termed informative biomarkers. Some biomarkers are diagnostic in combination with others. Individual biomarkers may be weighted when used in combinations. Biomarkers can be assessed in individual, isolated or assays, in parallel assays, or in single-pot assays. | 12-02-2010 |
20100304982 | SCAFFOLDED NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING - The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries. | 12-02-2010 |
20100311598 | SYSTEM AND METHOD FOR EDITING AND MANIPULATING DNA - A system and method for planning, manipulating, processing and editing DNA molecules utilizing a core operation on a given input DNA molecule to produce a targeted DNA molecule. | 12-09-2010 |
20100317532 | INHIBITION-BASED HIGH-THROUGHPUT SCREEN STRATEGY FOR CELL CLONES - A method for screening cells with high level expression of a target protein is disclosed. The method includes introducing into a plurality of host cells a DNA construct that encodes both a target protein and an inhibitor to an endogenous selectable marker in the host cells, screening host cells harboring the DNA construct for the expression of the endogenous selectable marker, and isolating cells with reduced expression of the selectable marker. Also disclosed is a DNA construct configured to express both the target protein and the inhibitor inside the host cell. | 12-16-2010 |
20100317533 | BIOMARKERS OF CANCER METASTASIS - There is provided a panel of biomarkers of tumour metastasis comprising any two of carbonic anhydrase-9 (CAIX), vascular endothelial growth factor C (VEGF-C), ephrin A5 (EFNA5), eph receptor B2 (EPHB2), transforming growth factor beta 3 (TGF-β3), pyruvate dehydrogenase kinase isoenzyme-3 (PDK3), carbonic anhydrase-12 (CAXII), keratin 14 (KRT14), hypoxia inducible factor 1 alpha subunit (HIF-1α), or tenascin C (TNC). CAIX, VEGF-C, EFNA5, EPHB2, TGF-β3 or PDK3 may be indicators of moderate metastatic potential, while CAXII, KRT14, HIF-1α, or TNC may be indicators of high metastatic potential. There is also provided a method of determining risk of tumour metastasis using the aforementioned biomarkers is also provided. The biomarkers may be used in diagnosis, prognosis, treatment selection, or to test putative therapeutics. The biomarkers may be used to assess malignancies or cancers having hypoxic regions, such as breast cancer. | 12-16-2010 |
20100317534 | GLOBAL GERM LINE AND TUMOR MICROSATELLITE PATTERNS ARE CANCER BIOMARKERS - The present invention includes a method of identifying an increase in microsatellite DNA from a genomic nucleic acid sample comprising: obtaining a microsatellite profile from a sample suspected of comprising cancer cells; comparing the microsatellite profile to a reference microsatellite profile from a reference genome; and determining in increase in the number of microsatellite DNAs from the sample as compared to the reference genome, wherein an increase in microsatellite DNA indicates a pre-disposition to cancer and the microsatellites are upstream from the estrogen receptor-related gamma gene (ESRRG). | 12-16-2010 |
20100317535 | Methods and Compositions For Detecting Nucleic Acid Molecules - There is provided methods and compositions for simultaneously detecting at least two mutually different nucleic acid molecules in a sample. In particular, the methods and composition may be employed in the multiplex detection of antibiotic resistance genes in bacteria. | 12-16-2010 |
20100323902 | Live-cell signals of pathogen intrusion and methods thereof - Disclosed is a system and method for measuring aspects of pathogen intrusion on a live-cell as defined herein. The system and method also provide a method to measure prophylaxis or remedial aspects of a therapeutic candidates in a live-cell or a live-cell model from pathogen intrusion. | 12-23-2010 |
20100323903 | DIAGNOSIS AND PROGNOSIS OF SPECIFIC CANCERS - The present invention provides nucleic acid sequences that are used for identification and diagnosis of specific cancers. The nucleic acid sequences can also be used for prognosis evaluation of a subject based on the expression profile of a biological sample. | 12-23-2010 |
20100331195 | Sequencing Nucleic Acid Polymers with Electron Microscopy - This invention relates to using an electron microscope to sequence by direct inspection of labeled, stretched DNA. This method will have higher accuracy, lower cost, and longer read length than current DNA sequencing methods. | 12-30-2010 |
20100331196 | ELECTRON BEAM NUCLEIC ACID SEQUENCING - The present invention relates to compositions, methods, and uses for obtaining sequence information from nucleic acid molecules. | 12-30-2010 |
20100331197 | SYSTEM AND METHOD FOR THE CLONAL CULTURE OF EPITHELIAL CELLS AND APPLICATIONS THEREOF - The invention relates to means and methods for evaluating and using the specific properties of a particular epithelial cell present in a biological sample. Accordingly, the invention relates to a system for the culture of epithelial cells, in which at least one clonal culture is sown with a single epithelial cell directly extracted from a biological sample of epithelial tissue. The invention also relates to a method for the culture of epithelial cells, that particularly comprises the production of clonal cultures, each being sown with a distinct and unique epithelial cell directly extracted from a biological sample of epithelial tissue, the evaluation of the cellular growth in the clonal cultures, and advantageously the analysis of the capacity of the cellular material from the clonal cultures to reconstruct a three-dimensional epithelium representative of native tissue. The invention is adapted for the parallel implementation of a very large number of clonal cultures, in particular for making large-scale tests. | 12-30-2010 |
20110003698 | MODIFICATION OF SELECTIVITY FOR SENSING FOR NANOSTRUCTURE SENSING DEVICE ARRAYS - An electronic system for selectively detecting and identifying a plurality of chemical species, which comprises an array of nanostructure sensing devices, is disclosed. Within the array, there are at least two different selectivities for sensing among the nanostructure sensing devices. Methods for fabricating the electronic system are also disclosed. The methods involve modifying nanostructures within the devices to have different selectivity for sensing chemical species. Modification can involve chemical, electrochemical, and self-limiting point defect reactions. Reactants for these reactions can be supplied using a bath method or a chemical jet method. Methods for using the arrays of nanostructure sensing devices to detect and identify a plurality of chemical species are also provided. The methods involve comparing signals from nanostructure sensing devices that have not been exposed to the chemical species of interest with signals from nanostructure sensing devices that have been exposed to the chemical species of interest. | 01-06-2011 |
20110003699 | Thermal Cycler for Microfluidic Array Assays - A system for thermal cycling a plurality of samples. The system includes a case having a fluid-tight cavity defining an interior volume. A microfluidic array is disposed in the interior volume, the array including a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of through-holes running through the thickness between the surfaces. A thermal cycler having at least one thermally controlled surface is adapted to thermally contact the case. | 01-06-2011 |
20110003700 | ELIMINATION OF CONTAMINANTS ASSOCIATED WITH NUCLEIC ACID AMPLIFICATION - Use of a non-natural base with an enzyme capable of degrading a nucleic acid containing a non-natural base in an amplification reaction to eliminate carry-over contaminants in the amplification reaction. | 01-06-2011 |
20110009276 | Method for Sequencing a Polynucleotide Template - The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. | 01-13-2011 |
20110009277 | METHOD FOR METHYLATION ANALYSIS - Aspects of the invention relate to composition and methods for the providing of DNA for methylation analysis that is in particular suitable to be applied in reference laboratories. Further 5 aspects of the invention relate to composition and methods for the highly specific and sensitive methylation analysis of the Septin 9 gene also in particular suitable to be applied in reference laboratories. | 01-13-2011 |
20110009278 | NUCLEIC ACID SEQUENCING SYSTEM AND METHOD - A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected. | 01-13-2011 |
20110009279 | Methods of Diagnosing Non-Alcoholic Steatohepatitis (NASH) - Non-invasive methods for detecting non-alcoholic fatty liver disease (NAFLD) and identifying the presence or absence of non-alcoholic steatohepatitis (NASH) in a subject utilize one or more biomarkers. The methods can differentiate between subjects with NASH and those with simple steatosis. Kits containing one or more agents for measuring the level of the biomarkers can be utilized to perform the described methods. | 01-13-2011 |
20110015081 | Genetic Variants on chr 5p12 and 10q26 as Markers for Use in Breast Cancer Risk Assessment, Diagnosis, Prognosis and Treatment - The invention pertains to certain genetic variants on Chr5p12 and Chr10q26 as susceptibility variants of breast cancer. Methods of disease management, including diagnosing increased and/or decreased susceptibility to breast cancer, methods of predicting response to therapy and methods of predicting prognosis using such variants are described. The invention further relates to kits useful in the methods of the invention. | 01-20-2011 |
20110015082 | Systems and Methods for Evaluating Operating Conditions in a Bioreactor Using Gene Expression and Abundance Tracking - Systems and methods for evaluating the operating conditions in a biological nitrogen removal reactor using gene expression and abundance tracking are disclosed. In some embodiments, the systems and methods include the following: obtaining a sample from the reactor during continuous reactor operation; expressing predetermined nitrification, denitrification, and structural genes for ammonia oxidizing bacteria contained in the sample to develop a sample genetic profile of the ammonia oxidizing bacteria; obtaining a genetic profile of a second bacteria substantially similar to the ammonia oxidizing bacteria, wherein the second bacteria was grown in a reactor having substantially optimum operating conditions; and comparing the sample genetic profile to the genetic profile of the second bacteria. | 01-20-2011 |
20110015083 | Identification of Orthologous Genes - A method is disclosed which can be used for the identification of one or more orthologous genes on a microarray, that utilises a microarray derived from a first animal that is used to analyse a corresponding nucleotide sequence from a second animal or a distinct variety of the first animal. The method comprises applying genomic DNA from the second animal, or distinct variety of the first animal, to a microarray derived from the first animal, measuring a background level of hybridisation intensity of the genomic DNA to probes of the microarray, and selecting probes on the microarray for which the hybridisation intensity is greater than a threshold value based on the background level of hybridisation intensity, whereby the selected probes are indicative of orthologous genes. | 01-20-2011 |
20110021361 | Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids - A method of sorting mixtures of nucleic acid strands comprising hybridizing the strands to an array of immobilized oligonucleotides, each of which includes a constant segment adjacent to a variable segment. The constant segment of the immobilized oligonucleotides can be made complementary to the ends of strands obtained by digesting a double-stranded nucleic acid with a restriction enzyme and restoring the restriction sites, thereby permitting the sorting of strands according to their variable sequences adjacent to their constant terminal restored restriction sites. | 01-27-2011 |
20110021362 | AGENTS FOR STIMULATING ACTIVITY OF METHYL MODIFYING ENZYMES AND METHODS OF USE THEREOF - Agents for stimulating activity of methyl modifying enzymes and methods of using the enzymes in assays to identify enzyme modulators are provided herein. | 01-27-2011 |
20110021363 | APPARATUS AND METHOD COMPRISING A SENSOR ARRAY AND A POROUS PLUNGER AND USE THEREOF - Disclosed is an apparatus that is used for detecting liquids or substances from liquids and includes a plunger that has a porous plunger layer which is pressed onto a sensor array. Each sensor of the sensor array is surrounded by elevations which spatially separate the sensors from each other like walls. In at least one embodiment, when the plunger layer is pressed onto the sensor array, the walls are pressed into the pores of the plunger layer. Liquid-tight connections are created between the walls and the plunger layer while liquid remains over the sensors. The liquid can be measured. In at least one embodiment, when there is direct mechanical contact between the plunger layer and the sensors, the liquid over the sensors is located in open pores on the surface of the plunger layer. No liquid flows between the pores and across the walls when pressure is applied to the plunger layer such that closed reaction chambers are created. | 01-27-2011 |
20110028331 | DETECTION OF MUTATIONS IN ACTA2 AND MYH11 FOR ASSESSING RISK OF VASCULAR DISEASE - A method of detecting in an individual an increased risk of hyperplastic vasculomyopathy, or a vascular disease resulting therefrom is disclosed. The method comprises obtaining a DNA genome sample from the individual and detecting in the sample a missense mutation in a gene which is a component of a smooth muscle cell contractile unit. In some embodiments the gene is ACTA2 and in some embodiments the gene is MYH11. In some embodiments, the gene is sequenced and then compared to a panel of control gene sequences which are representative of the same gene in individuals without vascular disease or who are at low risk of developing hyperplastic vasculomyopathy, to detect any missense mutations in the gene. The presence of a missense mutation in the gene indicates an increased risk of hyperplastic vasculomyopathy. | 02-03-2011 |
20110028332 | MARKER FOR DIAGNOSIS OF BREAST CANCER, TEST METHOD, AND TEST KIT - An embodiment of the present invention provides a marker, a test method, and a test kit which can detect the onset of breast cancer that cannot be detected by palpation or mammography examination or breast cancer in an early stage (clinical stage 0), which are simple, and which have high reliability. | 02-03-2011 |
20110034341 | METHOD AND DEVICE FOR DETERMINING THE RELEVANCE OF SAMPLE ARRAY PREPARATIONS - The invention relates to a method and a device ( | 02-10-2011 |
20110034342 | METHOD OF GENERATING AN OPTIMIZED, DIVERSE POPULATION OF VARIANTS - The disclosure relates to a method of generating a diverse set of variants to screen improved and novel properties within the variant population, a system for creating the diverse set of variants, and the variant peptides. | 02-10-2011 |
20110034343 | METHOD AND APPARATUS FOR DETECTING ELUTION OF SAMPLES - The invention relates to a method and apparatus for detecting elution of a sample from a sample substrate to incubation buffer contained in a sample well while the sample substrate is still within the well. The method comprises measuring light absorption of the contents of the sample well at a predefined wavelength or wavelength range, and determining, based on the absorption measurement, the degree of elution of the sample. According to the invention, a wavelength or wavelength range is used which is absorbed by at least one elutable component of the sample but transmitted by the sample substrate. The invention provides a reliable way of determining the degree of elution of blood samples in neonatal screening, for example. | 02-10-2011 |
20110039708 | MOLECULAR CLASSIFIER FOR PROGNOSIS IN MULTIPLE MYELOMA - The invention relates to a 15-gene molecular classifier consisting of the following genes: CNDP2; STMN1; AFG3L2; STK38; PARP1; CPSF6; LOC151162; C20orf100; FRY; FLJ21438; MGST1; ALDH2; CTSF; ATF4; FAM49A. The level of expression of these genes in bone marrow plasma cells of patients with multiple myeloma is a useful marker for evaluating their probability of survival. | 02-17-2011 |
20110039709 | APPARATUS FOR PROCESSING BIOLOGICAL MATERIAL - The invention relates to an apparatus for purification, respectively processing and/or analysis of biological target molecules with a detection device for detecting at least one object, which includes at least one detection area, wherein the detection device is adapted to detect at least one height value of the detection area and is adapted to determine, from the at least one height value, a spatial position and/or orientation and/or a type and/or a presence and/or a number and/or a state of the at least one object. Further, the invention relates to a receiving device for receiving material for the processing, purification and/or analysis of biological target molecules with at least one identification element, wherein the at least one identification element defines a height profile for identifying the receiving device, wherein the height profile is provided for at least one height measurement and extends at least in sections along a line, preferably a straight line. Finally, the invention relates to a method for inventorying, position determining and/or orientation determining of objects, which are needed for the automatic processing of a material, which contains biological target molecules, on a corresponding apparatus, in which at least one height value of a detection area of the respective object is detected, and, from the height value, a spatial position and/or an orientation and/or a type and/or a presence and/or a number and/or a state of the respective object is determined on the apparatus. | 02-17-2011 |
20110039710 | APPARATUS AND METHODS FOR APPLICATIONS OF GENOMIC MICROARRAYS IN SCREENING, SURVEILLANCE AND DIAGNOSTICS - This disclosure describes combinations of apparatus and methods to comprise a system for broad use and effective application of analytical genomic microarrays for screening and surveillance. The methodology abandons reliance on typical volumes of peripheral blood samples obtained by phlebotomy, in preference for protocols enabling collection, stabilization, archive, extraction and purification of small volumes as obtained from a finger prick. Recommended processing protocols from such starting material favor preparation of sufficient quantities of RNA or DNA of sufficient quality for subsequent steps of targeted amplification and fluorescent labeling. A strategy is offered for effective integration of capabilities for genotype and phenotype analysis on the same microarray layout. These gene expression re-sequencing arrays (GXR) are well suited for screening and surveillance applications. | 02-17-2011 |
20110039711 | THERMAL CONTROL SYSTEM AND METHOD FOR CHEMICAL AND BIOCHEMICAL REACTIONS | 02-17-2011 |
20110039712 | PREPARATION OF LIQUID MIXTURES - A method of determining the relative component proportions of at least one each of: a buffer; an acid or a base; a solvent; and optionally a salt, for providing a liquid mixture of pre-defined pH and ionic strength, wherein the relative component proportions are determined using the equation of Debye-Hückel, wherein the ion size parameter a in the Debye-Hückel equation is determined as the weighted mean ion size of all species contributing to the ionic strength of the liquid mixture, and wherein the ionic strength of each species is used as weighting parameter. The present method is also applicable in a method of providing a liquid mixture. Further there is provided a buffer preparation device. | 02-17-2011 |
20110045990 | STRUCTURAL NUCLEIC ACID GUIDED CHEMICAL SYNTHESIS - Disclosed is a composition comprising a nucleic acid and a chemical compound, said composition forming a star structure defining 3 or more stems extending from a reaction center. The stems are formed by a nucleic acid duplex and the chemical compound has been formed in the reaction center as the reaction product of 3 or more chemical groups. | 02-24-2011 |
20110045991 | Methods for the Detection of Colorectal Cancer - This invention relates to an approach for detection of chain truncating mutations based on the utilization of existing sample collection methods such as FOBT platforms, together with advanced methods for cell-free protein expression. “When further combined with mass spectrometry, the invention provides the ability to simultaneously detect changes in the amino acid sequence of multiple peptides. In some embodiments, DNA is isolated from a patient fecal sample and specific regions of a gene (i.e., for example, a K-ras gene or an APC gene) are PCR amplified using specifically designed primers that allow translation of encoded peptide fragments in a cell-free protein synthesis system. Nascent proteins are affinity purified and their mass is detected by MALDI-TOF which allows identifying low levels of mutations. | 02-24-2011 |
20110045992 | DNA SEQUENCING METHOD AND SYSTEM - The present invention relates to the field of gene engineering, provides a DNA sequencing method and system. Said DNA sequencing method includes following steps: A. said DNA is processed into multiple DNA segments, and then constructed into multiple DNA tags; B. amplification of every single DNA tag, and then processed into single stranded DNA; C. Utilize the anchor which can ligate to DNA tags and possesses at least one degenerated base to sequence every single DNA tag and thus produce sequencing signal; D. Obtain sequences of every single DNA segment by sequencing signal. Said DNA sequencing system, includes: unit of short tags construction, unit of amplification, unit of sequencing reaction and unit of signal processing. In this invention, since DNA tags are sequenced by using sequencing anchor with at least one degenerative abase, length of DNA tags that can be directly sequenced is extended. Both short and long DNA tags can be sequenced. Thus application of DNA sequencing is expanded. | 02-24-2011 |
20110045993 | MICROFLUIDIC DEVICE FOR ASSESSING OBJECT/TEST MATERIAL INTERACTIONS - An object/test material interaction microti iridic device comprising a sample inlet adapted to receive a fluid sample comprising a plurality of objects, an outlet adapted to output the fluid sample from the device, at least one internal surface defining a flow cavity within the device, wherein the flow cavity extends between and is connected to the sample inlet and the outlet for flow of the fluid sample through the flow cavity, the flow cavity comprises a test area to which at least one test material is attached and which is situated in the flow cavity for flow of the fluid sample over the test area, and the flow cavity has an aspect ratio which, when the flow cavity is substantially filled by the fluid sample, provides a substantially constant shear force between the test area and the fluid sample flowing over the test area. The invention further comprises a system incorporating the device, methods of use of the device and system, and methods of analysing interactions. | 02-24-2011 |
20110045994 | PARTICLE CAPTURE DEVICES AND METHODS OF USE THEREOF - The present invention provides a device and methods of use thereof in microscale particle capturing and particle pairing. This invention provides particle patterning device, which mechanically traps individual particles within first chambers of capture units, transfer the particles to second chambers of opposing capture units, and traps a second type of particle in the same second chamber. The device and methods allow for high yield assaying of trapped cells, high yield fusion of trapped, paired cells, for controlled binding of particles to cells and for specific chemical reactions between particle interfaces and particle contents. The device and method provide means of identification of the particle population and a facile route to particle collection. | 02-24-2011 |
20110045995 | Flow cytometry for high thorughput screening - The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus. | 02-24-2011 |
20110053783 | BIOACTIVE SURFACE FOR HEPATOCYTE-BASED APPLICATIONS - The invention provides a surface, wherein said surface comprises (i) a polymer substrate and (ii) sugar groups and peptide groups coupled to said substrate suitable for culturing hepatocytes. | 03-03-2011 |
20110053784 | Microfluidic Device and Methods of Using Same - A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses. | 03-03-2011 |
20110053785 | SAMPLE PROCESSING DEVICES - Methods and devices for thermal processing of multiple samples at the same time are disclosed. The sample processing devices provide process arrays that include conduits useful in distributing sample materials to a group of process chambers located in fluid communication with the main conduits. The sample processing devices may include one or more of the following features in various combinations: deformable seals, process chambers connected to the main conduit by feeder conduits exiting the main conduit at offset locations, U-shaped loading chambers, and a combination of melt bonded and adhesively bonded areas. | 03-03-2011 |
20110053786 | HIGH THROUGHPUT SCREENING EMPLOYING COMBINATION OF DISPENSING WELL PLATE DEVICE AND ARRAY TAPE - High throughput screening is performed by directing an array tape ( | 03-03-2011 |
20110059851 | SHOC2 Mutations Causing Noonan-Like Syndrome With Loose Anagen Hair - The present invention is directed to methods of diagnosing Noonan-like syndrome with loose anagen hair comprising detecting a mutation in SHOC2 gene. One specific diagnostic mutation disclosed is an A-to-G transition at position 4 resulting in a mutation at position 2 of SHOC2 amino acid sequence from serine to glycine. The invention also provides related sequences and kits. | 03-10-2011 |
20110059852 | COMPOSITIONS AND METHODS FOR TREATING HAEMATOLOGICAL PROLIFERATIVE DISORDERS OF MEYLOID ORIGIN - The disclosure relates to methods and compositions effective in the diagnosis, prognosis and treatment of human hematopoietic cancers. In particular, the disclosure provides tumor-associated genes that encode for cytokine receptors that are differentially expressed in hematopoietic tumor cells of myeloid origin compared with other cells, e.g., normal stem cells. | 03-10-2011 |
20110065590 | Method and Apparatus for Performing Microassays - A method and apparatus for analyzing a plurality of substances. A platen is provided having two substantially parallel planar surfaces and a plurality of through-holes. An optical arrangement analyzes light emanating from the through-holes. The through-holes may be individually addressable, and may have volumes less than 100 nanoliters. Samples may be accurately dispensed, diluted and mixed in accordance with embodiments of the invention, and may be plated onto walls of the through-holes, and then subsequently resuspended prior to characterization, or, alternatively, retained in the through-holes by surface tension. | 03-17-2011 |
20110065591 | BIOCHIP AND TARGET DNA QUANTITATIVE METHOD - A biochip used for quantitative analysis of a target DNA contained in a sample, includes a type I chamber that includes: a primer that is designed to bind to the target DNA; a internal standard DNA of a first amount that has a sequence different from a sequence of the target DNA, and is amplifiable with the primer; and a fluorescent probe that is designed to bind to a part of an PCR product of the target DNA and to a part of an PCR product of the internal standard DNA, and fluoresces differently for the PCR product of the target DNA and the PCR product of the internal standard DNA, and a type II chamber including: the internal standard DNA of a second amount different from the first amount; and the primer and the fluorescent probe. | 03-17-2011 |
20110065592 | RAPID METHOD FOR IDENTIFYING POLYPEPTIDE-NUCLEIC ACID INTERACTIONS - The invention relates to a method for analysing interactions of a nucleic acid with at least one polypeptide, comprising (a) providing a reaction vessel with a composition comprising cross-linked complexes comprising at least one nucleic acid and at least one polypeptide; (b) immobilizing at least part of the cross-linked complexes to the reaction vessel; (c) at least partially reversing said cross-links in said immobilized complexes by performing a heat incubation step thereby at least partially releasing the nucleic acids from the complexes; (d) analyzing the released nucleic acid. wherein steps (a) to (d) are performed in one reaction vessel. Also provided are specific reaction vessels and kits. | 03-17-2011 |
20110071031 | COMPOSITIONS, METHODS, AND KITS FOR ISOLATING AND ANALYZING NUCLEIC ACIDS USING AN ANION EXCHANGE MATERIAL - The present disclosure relates to methods for isolating, amplifying, and/or analyzing nucleic acids in the presence of an anion exchange material by performing the isolation, amplification and/or analysis step in the presence of at least one anionic compound. | 03-24-2011 |
20110071032 | Novel tumor marker determination - A method of determining CCNE2 in a body fluid sample of patients at risk for solid tumor disease. A multi-marker panel is preferably used for detecting circulating tumor cells, comprising CCNE2, DKFZp762E1312, EMP2, MAL2, PPIC, SLC6A8 and GTF2IRD1, and optionally further comprising one or more markers from the group consisting of AGR2, FXYD3, S100A16, TFF1, mammaglobin A, FN, Epcam, tm4sf and rbpms. | 03-24-2011 |
20110071033 | METHODS AND DEVICES FOR ASSESSING INFERTILITY AND/OR EGG QUALITY - The invention generally relates to methods and devices for assessing infertility. In certain embodiments, methods of the invention involve conducting an assay to determine presence or absence of a mutation in a plurality of genes selected from Table 1, and assessing infertility based on results of the assay, wherein presence of a mutation in at least two of the genes from Table 1 is indicative of infertility. | 03-24-2011 |
20110071034 | CLASSIFICATION AND DIAGNOSIS OF THE MOLECULAR BASIS OF CHOLESTASIS - The methods and compositions of the invention find use in the clinical diagnosis of cholestasis related syndromes, particularly PFIC types 1, 2, and 3; BRIC types 1 and 2; Alagille syndrome, and alpha 1-antitrypsin deficiency. The compositions of the invention include isolated nucleic acid molecules and oligonucleotide pairs suitable for use in amplifying regions of cholestasis related genes. Compositions of the invention include a cholestasis related gene resequencing microarray suitable for determining the nucleotide sequence of a region of a cholestasis related gene. Knowledge of the nucleotide sequence of one or more regions of a patient's cholestasis related gene allows diagnosis of the patient's syndrome. | 03-24-2011 |
20110071035 | Optical Apparatus - A method, optical apparatus and system for illuminating particles and for detecting emissions from illuminated particles in a flow cytometer. One configuration includes an objective lens coaxially aligned with the flow of particles being analyzed for collecting emissions at a detector. | 03-24-2011 |
20110071036 | ISOELECTRIC FOCUSING BIOCHIP - The present invention relates to an isoelectric focusing biochip, in particular for fractionating, detecting and/or collecting analytes, such as proteins, metabolites, glycoproteins and/or peptides, a method for fractionating, detecting and/or collecting analytes, with such a biochip and a manufacturing method for such a biochip. | 03-24-2011 |
20110071037 | Device and Method for Detecting Trace Gases - The invention relates to a gas detection system ( | 03-24-2011 |
20110077162 | ANALYTICAL MEASURING AND EVALUATION METHOD FOR MOLECULAR INTERACTIONS - The invention relates to an analytical measuring and evaluation method for determining the interaction parameters between an analyte and a ligand, preferably in a biosensor. According to the inventive method, the concentration of the analyte is gradually changed at defined intervals ti and the initial association or dissociation rates or association and dissociation rate constants are determined. The invention further relates to a device for carrying out the inventive method. | 03-31-2011 |
20110077163 | Reverse Transfection Of Cell Arrays For Structural And Functional Analyses Of Proteins - The present invention relates to articles and methods for determining the function of genes, gene products, and nucleic acid products. The present invention also relates to identifying ligands and binding partners or proteins and nucleic acid products. The present invention also relates to methods and compositions related to reverse-transfection. | 03-31-2011 |
20110086763 | METHODS FOR MULTIPLEX AMPLIFICATION - Methods for multiplex amplification of target nucleic acid sequences are provided. | 04-14-2011 |
20110086764 | SINGLE TUBE MULTIPLEX ASSAY FOR DETECTION OF ADULTERANTS IN BASMATI RICE SAMPLES - The present invention provides a single tube multiplex assay for distinguishing basmati from non-basmati rice varieties, and thereby identifying the adulteration of basmati rice varieties. The present invention further provides a method for quantifying adulteration in basmati rice varieties. The present invention also provides a kit for performing a multiplex assay for distinguishing basmati from non-basmati rice varieties. The kit may comprise a primer directed to an SSR loci, appropriate reagents for PCR, and optionally, a package insert for conducting the assay. | 04-14-2011 |
20110086765 | GENE EXPRESSION PROFILING OF PRIMARY BREAST CARCINOMAS USING ARRAYS OF CANDIDATE GENES - Method for molecular characterization of a carcinoma including: (i) detecting in tumor cells corresponding to breast tumor cells at least one polynucleotide selected from a first group determining expression level of the polynucleotide from the first group to differentiate a tumor in which a lymph node has been invaded by a tumor cell from a tumor in which a lymph node has not been invaded by a tumor cell; (ii) detecting in tumor cells corresponding to breast tumor cells at least one polynucleotide selected from a second group determining expression level of the polynucleotide from the second group to distinguish tumors sensitive to anthracycline from tumors insensitive to anthracycline; (iii) detecting in tumor cells corresponding to breast tumor cells at least one polynucleotide selected from a third group determining expression levels of the polynucleotide from the third group to classify good and poor prognosis primary breast tumors. | 04-14-2011 |
20110086766 | Chemical and Biological Detection Arrays - Device comprising: a first substrate; a plurality of first raised elements on the first substrate, the first raised elements mutually spaced apart by first channel regions on the first substrate, each of the first raised elements having a first distal end, the first distal ends forming a first array; hydrophobic molecules on the first raised elements; and primary reactive molecules on the first raised elements for generating hydrophilic reaction products. Techniques for utilizing the device. | 04-14-2011 |
20110092374 | METHODS FOR PRODUCING SUBSTANTIALLY HOMOGENEOUS HYBRID OR COMPLEX N-GLYCANS IN METHYLOTROPHIC YEASTS - The present invention provides methods for effectively and efficiently converting methylotrophic yeast's heterogeneous high mannose-type N-glycosylation to mammalian-type N-glycosylation by disruption of an endogenous glycosyltransferase gene (OCH1) and step-wise introduction of heterologous glycosidase and glycosyltransferase activities. Each engineering step includes a number of stages: transformation with an appropriate vector, cultivation of a number of transformants, performance of sugar analysis and heterologous protein expression analysis, and selection of a desirable clone. The selected clone is then subjected to the next engineering step. | 04-21-2011 |
20110092375 | Deducing Exon Connectivity by RNA-Templated DNA Ligation/Sequencing - A technology is described that is capable of generating high-throughput sequencing (HTS) read length DNA products to accurately and reliably provide exon connectivity information for alternatively spliced isoforms. The method is not limited by the initial size of the isoform as the technology removes the template oligonucleotide sequence and a newly formed full length ligated product provides an HTS-compatible read length sequence that comprises information that corresponds to the consecutive order of the exons in the original template oligonucleotide. | 04-21-2011 |
20110092376 | SYSTEM FOR DROPLET-BASED ASSAYS USING AN ARRAY OF EMULSIONS - System, including method and apparatus, for performing droplet-based assays. In the method, an emulsion may be obtained that includes droplets packed closely together in a three-dimensional packing arrangement. Data related to an analyte may be collected from individual droplets of the reacted emulsion as such droplets travel serially through a detection region. | 04-21-2011 |
20110092377 | High throughput methods for analysis of contamination in environmental samples - Use of high throughput methods to analyze samples for toxic elements originating from industrial hygiene and environmental sampling are described. These methods utilize optical detection methods using plates with arrays and microwells. Methods to fabricate samples in such plates are described. The invention is particularly illustrated by demonstrating its applicability for analysis of beryllium by fluorescence and uranium by phosphorescence. This invention also discloses the use of improved filtration method and use of reagents with low background signals. | 04-21-2011 |
20110092378 | ISOLATION AND USE OF SOLID TUMOR STEM CELLS - A small percentage of cells within an established solid tumor have the properties of stem cells. These solid tumor stem cells give rise to both more tumor stem cells and to the majority of cells in the tumor that have lost the capacity for extensive proliferation and the ability to give rise to new tumors. Thus, solid tumor heterogeneity reflects the presence of tumor cell progeny arising from a solid tumor stem cell. | 04-21-2011 |
20110098185 | COMPOSITION AND METHOD FOR DETERMINING OF ESOPHAGEAL CANCER - This invention relates to a composition, kit, or DNA chip for use in diagnosis of esophageal cancer, which comprises a plurality of polynucleotides selected from the group consisting of polynucleotides whose expression levels are varied in esophageal cancer tissues obtained from esophageal cancer patients when compared with cancer-free esophageal tissues obtained from esophageal cancer patients, mutants thereof, and fragments thereof, and to a method for detecting esophageal cancer using the composition, kit, or DNA chip. | 04-28-2011 |
20110105340 | USE OF GENE EXPRESSION SIGNATURES TO DETERMINE CANCER GRADE - Signatures indicative of cancer grades are based on over- and under-expression of 214 genes that characterize expression patterns in CD133 | 05-05-2011 |
20110105341 | Diagnostic Methods For Determining Prognosis Of Non-Small Cell Lung Cancer - The present disclosure provides methods for identifying early stage non-small-cell lung cancer (NSCLC) patients who will have an unfavorable prognosis for the recurrence of lung cancer after surgical resection. The methods are based in part on the discovery of chromosomal copy number abnormalities that can be used for prognostic classification. The methods preferably use fluorescence in situ hybridization with fluorescently labeled nucleic acid probes to hybridize to patient samples to quantify the chromosomal copy number of these genetic loci. | 05-05-2011 |
20110105342 | METHOD FOR DETERMINING REDUCED PREDISPOSITION TO CANCER BASED ON GENETIC PROFILE - The invention provide methods for early detection of a reduced risk of developing cancer, which comprises detecting the absence of a series of genetic polymorphisms associated with a predisposition of developing cancer, including the polymorphisms of the genes BRCA1, BRCA2, CARD15 (NOD2), CHEK2, CDKN2A (P16), CYP1B1, FGFR2 (KGFR2), MAP3K1 (MEKK1), p53 (TP53), TNRC9, XPD (ERCC2) and the genetic marker Rs6983267, in a biological sample from the analyzed subject, wherein the absence of the genetic polymorphisms is indicative of significantly decreased risk of developing, at least, breast cancer. | 05-05-2011 |
20110105343 | Systems Biology Approach Predicts Immunogenicity of Vaccines - A major challenge in vaccinology is to prospectively determine vaccine efficacy. Disclosed herein are methods and compositions for identifying early expression “signatures” that predicted immune responses in humans vaccinated with a vaccine. | 05-05-2011 |
20110105344 | Apparatuses for Detecting and Recognizing Analytes Based on Their Crystallization Patterns - The invention contemplates apparatuses for recognition of proteins and other biological molecules by imaging morphology, size and distribution of crystalline and amorphous dry residues in droplets (further referred to as “crystallization patterns”) containing predetermined amount of certain crystal-forming organic compounds (reporters) to which protein to be analyzed is added. Changes in the crystallization patterns of a number of amino-acids can be used as a “signature” of a protein added. Also, changes in the crystallization patterns, as well as the character of such changes, can be used as recognition elements in analysis of protein molecules. | 05-05-2011 |
20110111966 | ASSAYS FOR AGENTS AFFECTING MEGAKARYOCYTE DEVELOPMENT - Methods and kits for identifying candidate anti-megakaryocyte agents are disclosed. | 05-12-2011 |
20110111967 | METHOD FOR OPTIMIZED ISOLATION OF RNA FROM FIXED TISSUE - In invention relates to a method for the isolation of RNA from tissue pretreated with formaldehyde comprising homogenizing the sample in the presence of a guanidinium salt in aqueous solution, and incubating the sample in the presence of 0.1 M to 5 M ammonium salt at a temperature between 50° C. and 100° C. The heat treatment in the presence of an ammonium salts demodifies RNA by reverting methylol groups which are formed in the presence of formaldehyde between amino groups in nucleobases of RNA and in basic amino acids, and by cleavage of methylene bridges between amino groups in nucleobases of RNA and basic amino acids, to provide high RNA recoveries and consistently high quality of RNA for further reaction, e.g. for reverse transcriptase-polymerase chain reaction or microarray analysis. | 05-12-2011 |
20110111968 | Method and Apparatus for Melting Curve Analysis of Nucleic Acids in Microarray Format - A method and apparatus for performing melting curve analyses of nucleic acids on a microarray is described. The present method includes varying the temperature of a fluid on a microarray to dissociate and remove target DNA, scanning the mircoarray for fluorescence, collecting the target DNA removed from the microarray, and reusing the collected target DNA and the microarray. The apparatus of the present disclosure includes a microarray stage, a light source and detector, and a temperature controller, wherein the temperature controller is configured to adjust the temperature of a fluid within a sample chamber on the microarray such that the temperature of the fluid is varied during the analysis such that target DNA is dissociated from the microarray, and wherein the light source is directed to the microarray and the resulting fluorescence is perceived by the detector. | 05-12-2011 |
20110118127 | MARKER GENES FOR SCREENING OF DRUG-INDUCED TOXICITY IN HUMAN CELLS AND SCREENING METHOD USING THE SAME - The present invention relates to a marker gene for screening a drug inducing toxicity in human and a screening method using the same. More precisely, the invention relates to a microarray on which marker genes up-or down-regulated specifically by 16 drugs inducing pulmonary toxicity, teratogenicity, nephrotoxicity, cardiotoxicity or mutation (Methotrexate, Nitrofurantoin, Amiodarone, Carbamazepine, Valproic acid, Thalidomide, Cisplatin, Gentamycin, Amphotericine, Furylfuramide, N-nitroso-N-methylurea, methylmethanesulfonate, 4-nitroquinoline-N-oxide, 2-nitrofluorene, Doxorubicin and Daunorubicin) are integrated, a kit comprising the said microarray, and a screening method of a drug inducing toxicity in human using the same. The DNA microarray containing the marker gene of the present invention facilitates the construction of Toxtarget Array for screening a drug inducing toxicity in human using drug-specific genes, suggesting that this chip can be effectively used for monitoring drugs or chemicals carrying toxicity to human or determining risks thereof and also it can be used as a tool for examining mechanisms of toxicity/side effects caused by the drugs. | 05-19-2011 |
20110118128 | BIOSENSOR DEVICE AND METHOD OF MANUFACTURING THE SAME - A biosensor device ( | 05-19-2011 |
20110118129 | METHOD FOR PREDICTING THERAPY RESPONSIVENESS IN BASAL LIKE TUMORS - The present invention is related to a method for predicting a clinical response of a patient suffering from or at risk of developing a neoplastic disease towards at least one given mode of treatment, said method comprising the steps of: a) obtaining a biological sample from said patient; b) determining, on a non protein basis, the expression level of at least one gene of interest, said gene being correlated with the Estrogen receptor (ESR) status in the said sample, c) comparing the pattern of expression levels determined in (b) with one or several reference pattern(s) of expression levels; and d) predicting therapeutic success for said given mode of treatment in said patient from the outcome of the comparison in step (c). Moreover, the invention concerns nucleic acid molecules usefuls as primers and probes for detecting the expression of Her2-neu/ErbB2, MLHP and MMP7, respectively. | 05-19-2011 |
20110118130 | Compositions and methods for defining cells - Disclosed are compositions and methods for classifying stem cells. The disclosure provides a way to define cells using a computational analysis. | 05-19-2011 |
20110118131 | METHODS AND MATERIALS FOR TARGETED MUTAGENESIS - The present disclosure relates to methods and materials for mutagenesis, including for the generation of novel or improved proteins and libraries or arrays of mutant proteins or nucleic acids encoding such mutant proteins. | 05-19-2011 |
20110118132 | Enzymatic Assays Using Umbelliferone Substrates with Cyclodextrins in Droplets of Oil - The invention is directed to droplet actuator devices and assay methods. The method may include, among other things, a method of conducting enzymatic assays using umbelliferone substrates with cyclodextrins in droplets in oil, the method including incubating a droplet in oil, the droplet preferably comprising an umbelliferone substrate, a sample, and a cyclodextrin compound. The methods may further include a method of substantially eliminating cross-contamination between droplets in enzymatic containing substrate-based bioassays. The method may include immobilization of the enzymatic substrate including forming an inclusion complex with the substrate for stabilizing the substrate within an aqueous environment, wherein the inclusion complex may be formed using cyclodextrins. In yet another embodiment, the invention may provide a method of enhancing hydrolysis of enzymatic substrates. The methods may further include a method of forming an inclusion complex with the substrate for stabilizing the substrate within an aqueous environment, wherein the substrate may be a 4-MU- or HMU containing substrate. The inclusion complex may be formed using cyclodextrin. | 05-19-2011 |
20110118133 | METHODS TO IDENTIFY PATIENTS AT RISK OF DEVELOPING ADVERSE EVENTS DURING TREATMENT WITH ANTIDEPRESSANT MEDICATION - The invention provides a method of screening patients to identify those patients more likely to exhibit an increased risk of treatment-emergent suicidal ideation comprising: (a) obtaining a sample of genetic material from the patients, and (b) assaying the sample for the presence of a genotype in the patients which is associated with an increased risk of treatment-emergent suicidal ideation, wherein the genotype is characterized by a polymorphism in a gene selected from the group consisting of glutamine receptor, ionotropic, kainate 2 (GRIK2); glutamate receptor ionotropic AMPA 3 (GRIA3); and combinations thereof. | 05-19-2011 |
20110124510 | METHODS OF LABELING POLYNUCLEOTIDE WITH DIBENZORHODAMINE DYES - Dibenzorhodamine compounds having the structure | 05-26-2011 |
20110124511 | Gene Expression Profile-Facilitated In Vitro Fertilization - Gene expression profiling improves the pregnancy success rate of in vitro fertilization processes, while reducing the risk of multiple births. | 05-26-2011 |
20110124512 | METHODS AND KITS FOR SUSTAINING eNOS ACTIVITY TO INHIBIT PLATELET AGGREGATION, AND CLOT RETRACTION, AND PROMOTE FIBRINOLYSIS - The present invention is directed to methods for sustaining eNOS activity to inhibit platelet aggregation, clot retraction, and enhance fibrinolysis. One embodiment of the invention provides methods of treating thrombosis by inhibiting the activity of the syk kinase. Another embodiment provides assays for the discovery of improved compounds to treat thrombosis, by screening for compounds which sustain eNOS activity. Yet another embodiment provides assays for the discovery of improved compounds to treat thrombosis, by identifying inhibitors of calpain and IIbIIIa by screening for compounds which act through calpain or IIbIIIa to sustain eNOS activity. Yet another embodiment provides for enhancing fibrinolysis, by inhibiting the activity of the syk kinase or calpain. | 05-26-2011 |
20110124513 | PROFILES OF VOLATILE HYDROCARBONS, MARKERS OF DEGRADATION/AGEING OF CELLULOSIC MATERIAL AND MARKERS OF PATHOLOGY - The present invention relates to profiles (significant set) of volatile organic compounds, VOCs, namely hydrocarbons between 2 and 24 carbon atoms (C | 05-26-2011 |
20110130295 | Diagnostic Methods For Determining Prognosis Of Non-Small Cell Lung Cancer - Disclosed are methods for identifying early-stage non-small-cell lung cancer (NSCLC) patients who will have an unfavorable prognosis for the recurrence of lung cancer after surgical resection. The methods are based in part on the discovery that chromosomal copy number gains at Chr19, 34.7 Mb-35.6 Mb can be used for prognostic classification. The methods preferably use fluorescence in situ hybridization with fluorescently labeled nucleic acid probes to hybridize to patient samples to quantify the chromosomal copy number of this genetic locus. | 06-02-2011 |
20110130296 | MULTI-GENE CLASSIFIERS AND PROGNOSTIC INDICATORS FOR CANCERS - The present invention relates to the identification of marker genes useful in the diagnosis and prognosis of clinically problematic subsets of primary breast cancers. More specifically, the invention relates to the identification of two sets of marker genes that are differentially expressed in and useful for the diagnosis and prognosis of subsets of hormone receptor-negative (HRneg; i.e., ER and PR negative) and triple-negative (Tneg; i.e., ER, PR and HER2 negative) primary breast cancers at highest risk for early metastatic relapse. The invention further provides methods for determining the best course of treatment for patients having one of these clinically problematic subsets of primary breast cancers. The invention also provides methods for identifying compounds that prevent or treat a subtype of breast cancer based on their ability to modulate the activity or expression level of one or more marker genes identified herein. | 06-02-2011 |
20110130297 | QUANTUM DOT-SENSORY ARRAY FOR BIOLOGICAL RECOGNITION - The present invention provides a quantum dot-based biomolecule sensor array capable of differentiating the strain of a variety of biological molecules including bacteria, spores, fungi, viruses, and disease-causing prions. The biosensor uses specific chemical functionalities that regulate the interactions between different chemical ligands and biological molecules. | 06-02-2011 |
20110130298 | METHOD OF PREDICTING/OPTIMIZING HYDROGEN SULFIDE SCAVENGING CAPACITY AND REDUCTION OF SCALE FORMATION - A method of optimizing a hydrogen sulfide scavenger blend that includes selecting a hydrogen sulfide scavenger blend comprising at least two hydrogen sulfide scavengers; determining a scavenging capacity for the blend; modifying at least one blend parameter based on the determined scavenging capacity; redetermining the scavenging capacity for the modified blend; and selecting an optimized blend from the blend and the modified blend is disclosed. | 06-02-2011 |
20110136678 | METHODOLOGY FOR ANALYSIS OF SEQUENCE VARIATIONS WITHIN THE HCV NS3/4A GENOMIC REGION - The current invention relates to a standardized method for amplification of an HCV NS3/4A or NS3 nucleic acid fragment of any one of HCV genotypes 5 to 6 or HCV genotype 1 as a tool for analysis of sequence variations that may be correlated with 5 HCV drug resistance. | 06-09-2011 |
20110136679 | Imprinted Array - There is described a gene expression array comprising more than one imprinted gene and one or more control genes for the diagnosis of human epigenetic diseases resulting from assisted reproduction or the disruption. | 06-09-2011 |
20110136680 | TUMOR GRADING AND CANCER PROGNOSIS - The disclosure includes the identification and use of gene expression profiles, or patterns, with clinical relevance to cancer. In particular, the disclosure includes the identities of genes that are expressed in correlation with tumor grade. The levels of gene expression are disclosed as a molecular index for determining tumor grade in a patient and predicting clinical outcome, and so prognosis, for the patient. The molecular grading of cancer may optionally be used in combination with a second molecular index for diagnosing cancer and its prognosis. The disclosure further includes methods for predicting cancer recurrence, and/or predicting occurrence of metastatic cancer. For diagnosis or prognosis, the disclosure further includes methods for determining or selecting the treatment of cancer based upon the likelihood of life expectancy, cancer recurrence, and/or cancer metastasis. | 06-09-2011 |
20110136681 | SELECTION OF STEM CELL CLONES WITH DEFINED DIFFERENTIATION CAPABILITIES - Disclosed are methods for producing a clonal population of cells involving: a) obtaining a population of pluripotent or multipotent cells that have been expanded in vitro and maintained in an undifferentiated or essentially undifferentiated state; b) expanding individualized cells of the population into clonal populations of cells; and c) selecting one or more clonal population of cells determined to have the ability to differentiate into a population that is at least about 50% homogeneous for either neural cell types, hepatocytes, or cardiomyocytes. Also disclosed are clonal populations of cells produced by the methods of the present invention, and methods of treating disease in subjects involving administration of clonal cells of the present invention to a subject. Methods of screening test compounds that involve contacting a test compound with a clonal population of cells produced by the methods of the present invention are also set forth. | 06-09-2011 |
20110136682 | ANTENNARY FUCOSYLATION IN GLYCOPROTEINS FROM CHO CELLS - The present invention provides methods of evaluating CHO cells and producing recombinant glycoproteins. | 06-09-2011 |
20110136683 | Systems and Methods for Expression-Based Discrimination of Distinct Clinical Disease States in Prostate Cancer - A system for expression-based discrimination of distinct clinical disease states in prostate cancer is provided that is based on the identification of sets of gene transcripts, which are characterized in that changes in expression of each gene transcript within a set of gene transcripts can be correlated with recurrent or non-recurrent prostate cancer. The Prostate Cancer Prognostic system provides for sets of “prostate cancer prognostic” target sequences and further provides for combinations of polynucleotide probes and primers derived there from. These combinations of polynucleotide probes can be provided in solution or as an array. The combination of probes and the arrays can be used for diagnosis. The invention further provides further methods of classifying prostate cancer tissue. | 06-09-2011 |
20110136684 | CHEMICAL REACTION MONITOR - Disclosed herein are systems for monitoring chemical reactions. The systems can comprise a lighting device, a camera device for obtaining an image of the chemical reaction mixtures and an analyzer program to process data obtained from the image. Also disclosed are methods of monitoring the progress of chemical reactions using these systems. | 06-09-2011 |
20110136685 | METHOD FOR DETERMINING A PREDISPOSITION TO BASAL CELL CARCINOMA AND FOR SCREENING TREATMENTS THEREOF - The present invention relates to methods for detecting a predisposition to a basal cell carcinoma and methods for screening a treatment of a basal cell carcinoma. | 06-09-2011 |
20110143946 | Method for predicting the response of a tumor in a patient suffering from or at risk of developing recurrent gynecologic cancer towards a chemotherapeutic agent - The present invention relates to a method for predicting a response of a tumor in a patient suffering from or at risk of developing recurrent gynecologic cancer towards a chemotherapeutic agent, said method comprising the steps of: a) obtaining a biological sample from said patient; b) determining the pattern of expression level of at least one gene of the group comprising AKR1C1, MLPH, ESR1, PGR, COMP, DCN, IGKC, CCL5, FBN1 and/or UBE2C, or of genes coregulated therewith, in said sample; c) comparing the pattern of expression levels determined in (b) with one or several reference pattern (s) of expression levels; d) identifying at least one marker gene; e) determining a molecular subtype for said sample on the basis of (d); and f) predicting from said molecular subtype response of a tumor for a chemotherapeutic agent, wherein the molecular subtype is selected from the group comprising the subtypes basal, stromal-high, stromal-low, luminal A, immune system-high, immune system-low, proliferation-high and/or proliferation-low. | 06-16-2011 |
20110143947 | Assay Apparatuses, Consumables and Methods - We describe apparatuses, method, reagents, and kits for conducting assays as well as process for their preparation. They are particularly well suited for conducting automated sampling, sample preparation, and analysis in a multi-well plate assay format. For example, they may be used for automated analysis of liquid samples in a clinical point of care setting. | 06-16-2011 |
20110143948 | MOLECULAR SIGNATURES AND BIOMARKERS ASSOCIATED WITH MELANOMA AND METHODS OF USE THEREOF - Described herein are methods for evaluating the risk of melanoma in subjects. The methods involve detecting and quantifying one or more biomarkers associated with melanoma in a biological sample from a subject. | 06-16-2011 |
20110143949 | ANALYSIS USING MICROFLUIDIC PARTITIONING DEVICES - The invention relates to methods, reagents and devices for detection and characterization of nucleic acids, cells, and other biological samples. Assay method are provided in which a sample is partitioned into sub-samples, and analysis of the contents of the sub-samples carried out. The invention also provides microfluidic devices for conducting the assay. The invention also provides an analysis method using a universal primers and probes for amplification and detection. | 06-16-2011 |
20110143950 | Diagnosis/Treatment Option for Head-and-Neck Tumor Using Micro-RNA as Biomarker - Disclosed are: a method for using a particular microRNA as a biomarker for head-and-neck tumor; a method for the determination of head-and-neck tumor; a kit for the determination of head-and-neck tumor, and the like. The present invention is characterized in that at least one microRNA selected from the group of microRNAs consisting of miR-455-3p, miR-455-5p, miR-130b, miR-130b*, miR-801, miR-196a, miR-21, miR-31, miR-133b, miR-145 and miR-375 is used as a biomarker for head-and-neck tumor. | 06-16-2011 |
20110143951 | MASS MARKERS AND METHODS - The invention describes compounds useful for labelling molecules of interest (i.e. analytes), particularly biomolecules such as peptides, proteins, oligonucleotides and nucleic acids, and also methods for analysing, detecting and/or isolating these labelled molecules using mass spectrometry. The compound in one aspect is a mass marker for labelling of an analyte detectable by mass spectrometry such as neutral loss mass spectroscopy, in which the mass marker comprises a neutral loss mass modifier linked via a first collision cleavable linker to a reactive group having reactive functionality for attachment to the analyte. The neutral loss mass modifier upon cleavage from the analyte during mass spectroscopy is uncharged. | 06-16-2011 |
20110152106 | CST1, DCC1, IFITM1 OR MELK AS MARKERS FOR DIAGNOSING STOMACH CANCER - The present invention relates to a biomarker for gastric cancer, CST1 (cysteine proteinase inhibitor, type 2 family), DCC1 (Defective in sister chromatid cohesion homolog 1), IFITM1 (interferon induced transmembrane protein 1) or MELK (maternal embryonic leucine zipper kinase). More particularly, the present invention relates to a diagnostic composition for gastric cancer comprising an agent measuring the expression level of CST1, DCC1, IFITM1 or MELK, a kit comprising the composition, a method for detecting the marker, and a method for screening a therapeutic agent for gastric cancer using the marker. | 06-23-2011 |
20110152107 | Drug Screening Via Nanpore Silicon Filters - The invention provides a porous nanoscale membrane. In one embodiment, the membrane can be used as a filtration device to screen agents that disrupt or prevent molecular interactions. In one embodiment, the membrane allows for screening agents that disrupt or prevent molecular interactions using a small sample volume with efficient high-throughput screening applications. | 06-23-2011 |
20110152108 | Devices and Methods for Thermally-Mediated Chemical Reactions - One aspect of the invention provides container for thermal cycling a plurality of samples in a microfluidic array. The container includes a plurality of walls defining an interior volume and a conductive member for heating the interior volume. Another aspect of the invention provides container for thermal cycling a plurality of samples in a microfluidic array. The container includes a plurality of walls defining an interior volume and a plurality of conductive members for heating an interior volume. Another aspect of the invention provides a container for thermal cycling a plurality of samples in a microfluidic array. The container includes a plurality of walls defining an interior volume and a first conductive member located in the interior volume and adapted to contact a first end of the microfluidic array. | 06-23-2011 |
20110160067 | High throughput system for isolation, growth, and detection of lipid inclusions in bacteria - A method of high throughput growth and quantitative analysis of microorganisms is provided that includes providing a microtiter plate growth and gas delivery system having well plates disposed for growth of the microorganisms, and providing a spectroscopic screening system disposed to analyze lipid inclusions of the microorganisms. | 06-30-2011 |
20110160068 | AUTOMATED SEED SAMPLING APPARATUS, METHOD AND SYSTEM - An automated high throughput apparatus, method and system for seed sampling is provided. The seed sampling system includes an ablation subsystem having a controlled cutting path and a seed handling subsystem comprising a staging system having a plurality of seed staging positions for staging a batch of seed in a sequential manner, and a carrier system for simultaneously presenting seed staging positions at the cutting path for removing a sample portion from each seed in the batch. A method of this invention includes providing ablation means having a controlled cutting path, automatically staging a batch of seed at a plurality of staging positions in a sequential manner, simultaneously presenting the batch of seed at the cutting path, and removing a sample portion from each seed in the batch. | 06-30-2011 |
20110160069 | ANALYTE DETECTION USING A NEEDLE PROJECTION PATCH - Apparatus for use in detecting analytes in a subject, wherein the apparatus includes a number of projections provided on a patch, such that applying the patch to the subject causes at least some of the projections to be inserted into the subject and target one or more analytes and a reagent for detecting the presence or absence of analytes. | 06-30-2011 |
20110166028 | METHODS FOR PREDICTING TREATMENT RESPONSE BASED ON THE EXPRESSION PROFILES OF BIOMARKER GENES IN NOTCH MEDIATED CANCERS - The invention relates to the identification and use of gene expression profiles with clinical relevance to the treatment of cellular proliferative disorders, especially those mediated by aberrant Notch signaling using a Notch signaling inhibitor. In particular, the invention provides the identities of genes, whose individual or cumulative expression patterns may be useful in various assays. The gene expression profiles, whether embodied in nucleic acid expression, protein expression, or other expression formats, may be used to select subjects afflicted with a Notch mediated cancer who will likely respond to treatment with a gamma-secretase inhibitor or another Notch inhibiting agent. The same markers may be used in the classification of patients being treated with other Notch inhibitors. The methods may further comprise providing diagnostic, prognostic, or predictive information based on the classifying step. The methods may further comprise selecting a treatment based on the classifying step. | 07-07-2011 |
20110166029 | Compositions And Methods For Diagnosing Autism Spectrum Disorders - The invention generally relates to compositions and methods for diagnosing autism spectrum disorders. In certain embodiments, the invention provides a method for diagnosing presence or increased risk of developing an autism spectrum disorder in a subject. | 07-07-2011 |
20110166030 | Prediction of response to docetaxel therapy based on the presence of TMPRSSG2:ERG fusion in circulating tumor cells - A method for predicting with high specificity from RTPCR detected markers if a patent is likely to respond to docetaxel treatment is disclosed. RTPCR detects the presence of absence of certain mutations due to fusion events. Together, with other protein markers, additional stratification is possible to identify patents suited for docetaxel therapy as opposed to for alternative treatments. | 07-07-2011 |
20110166031 | Apparatus and Methods for Performing Real Time PCR in Array Tape - A carrier tape ( | 07-07-2011 |
20110172107 | ASSAY FOR IDENTIFYING AGENTS THAT MODULATE EPIGENETIC SILENCING, AND AGENTS IDENTIFIED THEREBY - A high throughput RNAi-based assay for identify factors involved in maintaining epigenetic silencing is disclosed. The assay measures reactivation of a silent reporter gene in cells, resulting from RNAi-based knockdown in target mRNA. RNAi-based screening of these silent reporter cells has identified known enzymes that place or remove epigenetic marks on histones, as well as non-enzymatic proteins that function in silencing or in transfer of marks during S-phase. In addition, the screen has been used to identify a number of novel gene products involved in epigenetic silencing, which are also disclosed. | 07-14-2011 |
20110172108 | TARGETING ENZYMES OF THE tRNA SPLICING PATHWAY FOR IDENTIFICATION OF ANTI-FUNGAL AND/OR ANTI-PROLIFERATIVE MOLECULES - The present invention relates to a method for screening and identifying compounds that modulate the activity of one or more components in the tRNA splicing pathway. In particular the invention relates to a method for screening and identifying compounds that modulate the activity tRNA splicing endonuclease and/or tRNA splicing ligase. The invention provides assays for the identification of compounds that inhibit animalia tRNA splicing endonuclease and/or animalia tRNA splicing ligase. The invention also provides assays for the identification of compounds that inhibit fungal tRNA splicing endonuclease and/or fungal tRNA splicing ligase. The methods of the present invention provide a simple, sensitive assay for high-throughput screening of libraries of compounds to identify pharmaceutical leads useful for treating and/or preventing cancer and/or fungal infections. | 07-14-2011 |
20110172109 | DEVICE FOR THE MANIPULATION OF LIMITED QUANTITIES OF LIQUIDS - Holding device for the arrangement of at least one optical component in front of a laser light source of a laser unit, including a first holding part to which at least one optical component is attached, the holding device furthermore including a second holding part which is attached to one part of the laser unit, and the first holding part being attached to the second holding part. Furthermore this invention relates to an arrangement with such a holding device and a process for producing this arrangement. | 07-14-2011 |
20110177955 | MULTIPLEXED PROTEIN ADSORPTION ASSAY - A multiplexed assay method capable of measuring the interaction of one or more protein, polypeptide or peptide solutions with one or more substrate surfaces comprises contacting each of the wells of a multiwell substrate with the same or different protein solution, the surfaces of said wells being the same as that of said substrate or being substrate surface treated and/or coated to provide test surfaces, and determining the level of protein adsorption in each of said wells. | 07-21-2011 |
20110177956 | METHOD AND SYSTEM OF PREDICTING CLINICAL OUTCOME FOR A PATIENT WITH CONGESTIVE HEART FAILURE - A method of predicting a clinical outcome for a patient with congestive heart failure is disclosed. A plurality of nonlinear first PCI models are identified based on a biomarker dataset, each of the models having a number of distinct terms. One or more second PCI models are identified based on the biomarker dataset, each of these models having a number of distinct terms which corresponds to the number of distinct terms for one or more of the nonlinear first PCI models. Each of the plurality of nonlinear first PCI models are statistically compared to one of the one or more second PCI models having a corresponding number of distinct terms to determine a preference for higher versus lower degree of nonlinearity or preference for shorter versus longer memory length. The clinical outcome is predicted based on the preference for higher versus lower degree of nonlinearity or memory length preference. | 07-21-2011 |
20110177957 | Polymorphisms Associated With Age-Related Macular Degeneration And Methods For Evaluating Patient Risk - The present invention provides for certain polynucleotide sequences that have been correlated to AMD. These polynucleotides are useful as diagnostics, and are preferably used to fabricate an array, useful for screening patient samples. The array is used as part of a laboratory information management system, to store and process additional patient information in addition to the patient's genomic profile. As described herein, the system provides an assessment of the patient's risk for developing AMD, risk for disease progression, and the likelihood of disease prevention based on patient controllable factors. | 07-21-2011 |
20110177958 | METHODS AND MATERIALS FOR IDENTIFYING POLYMORPHIC VARIANTS, DIAGNOSING SUSCEPTIBILITIES, AND TREATING DISEASE - The invention is directed to materials and methods associated with polymorphic variants in two enzymes involved in folate-dependent and one-carbon metabolic pathways: MTHFD1 (5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase) and methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L). Diagnostic and therapeutic methods are provided involving the correlation of polymorphic variants in MTHFD1, MTHFD1, and other genes with relative susceptibility for various pregnancy-related and other complications. | 07-21-2011 |
20110183856 | Diagnosis and Prognosis of Infectious Disease Clinical Phenotypes and other Physiologic States Using Host Gene Expression Biomarkers In Blood - The present invention provides a specific set of gene expression markers from peripheral blood leukocytes that are indicative of a host response to exposure, response, and recovery infectious pathogen infections. The present invention further provides methods for identifying the specific set of gene expression markers, methods of monitoring disease progression and treatment of infectious pathogen infections, methods of prognosing the onset of an infectious pathogen infection, and methods of diagnosing an infectious pathogen infection and identifying the pathogen involved. | 07-28-2011 |
20110183857 | NOVEL HUMAN SINGLE NUCLEOTIDE POLYMORPHISMS - Disclosed are methods for human identification utilizing newly discovered single nucleotide polymorphisms (SNPs) within CODIS loci which can cause allelic dropout. Also disclosed are kits useful in human identification. | 07-28-2011 |
20110183858 | GRADING OF BREAST CANCER - Methods and compositions for the identification of breast cancer grade signatures are provided. The signature profiles are identified based upon multiple sampling of reference breast tissue samples from independent cases of breast cancer and provide a reliable set of molecular criteria for identification of cells as being in one or more particular stages and/or grades of breast cancer. | 07-28-2011 |
20110183859 | INFLAMMATORY GENES AND MICRORNA-21 AS BIOMARKERS FOR COLON CANCER PROGNOSIS - Disclosed herein are methods for detecting a more aggressive form of a colon adenocarcinoma in a subject, thereby predicting the prognosis of the subject. The methods generally include determining an inflammatory gene expression signature in the colon adenocarcinoma and/or the adjacent non-cancerous tissue. In some embodiments, the inflammatory genes include, but are not limited to, PRG1, ANXA1, IL-17a, IL-23a FOXP3, HLA-DRA, IL-10, CD68 and IL-12a. In some embodiments, the method further includes detecting expression of microRNA-21 (miR-21) in the colon adenocarcinoma. Altered expression of one or more of the inflammatory genes or miR-21 indicates the prognosis of the subject. Also provided are arrays consisting essentially of probes specific for PRG1, ANXA1, IL-17 | 07-28-2011 |
20110190142 | DETERMINATION OF RENIN-PRORENIN RECEPTOR ACTIVITY - The present invention refers to the renin/prorenin receptor (RER) signal transduction pathway and, in particular, to the role of promyelocytic zinc finger protein (PLZF) and its downstream targets involved in this pathway, e.g. the p85α subunit of phosphatidylinositol-3 kinase (PI3K-p85α). In more detail, the present invention refers to a method for determination of RER activity, e.g. stimulation or inhibition of RER activity, using PLZF activity as a measurement from which RER activity is derived. For the determination of RER activity, use can be made of RER/PLZF protein interaction, PLZF translocation and/or PLZF recruitment. The present invention further refers to a use of said method for identifying RER ligands, e.g. pharmaceutically active agonists or antagonists, as well as for studying undesired side-effects of renin inhibitors. | 08-04-2011 |
20110190143 | Methods and Kits for the Rapid Determination of Patients at High Risk of Death During Septic Shock - The present invention relates to the field of treatment of serious medical syndromes such as severe sepsis and septic shock. In particular, the present invention provides methods and kits to obtain an early evaluation of mortality risk and help therapeutic decisions for patients in severe sepsis with two organ failures, for example for patients in septic shock with one additional organ failure. The methods of the invention are based on the analysis of an expression profile of one or several genes selected in the group consisting of HLA-DRB4, FOSB, GPR109B, RBP7, TLR7, IL15, HLA-C, AMFR, LOC96610, IRF5, MGC29506, EDG3, IGLV1-44, IFIT2, IFI44, EPSTI1, TNFRSF17, AREG, THBS1, CTLS1, TFPI, PHACTR2, PFKFB2 and CHIT1. | 08-04-2011 |
20110190144 | NOVEL COMPOSITIONS AND METHODS FOR THE TREATMENT OF PSORIASIS - The present invention relates to compositions containing a novel protein and methods of using those compositions for the diagnosis and treatment of psoriasis. | 08-04-2011 |
20110190145 | IN SITU ANALYSIS OF TISSUES - The present invention provides for the simultaneous assessment of a plurality of tissue regions or microregions, the benefit being homogeneity of the sampling, both in terms of tissue content and timing. Discrete regions of a tissue sample, such as those demarcated by microwells formed within the tissue itself or tissue plugs removed from the tissue in a spatially referenced fashion, can be treated with one or more physical or chemical treatments to liberate target molecules of interest. Subsequent analysis of said target molecules by, e.g., mass spectroscopy, permits identification of a variety of biological parameters, including those associated with disease or therapy. | 08-04-2011 |
20110190146 | MICROFLUIDIC DEVICE FOR STORAGE AND WELL-DEFINED ARRANGEMENT OF DROPLETS - The present invention relates to systems and methods for the arrangement of droplets in pre-determined locations. Many applications require the collection of time- resolved data. Examples include the screening of cells based on their growth characteristics or the observation of enzymatic reactions. The present invention provides a tool and related techniques which addresses this need, and which can be used in many other situations. The invention provides, in one aspect, a tool that allows for stable storage and indexing of individual droplets. The invention can interface not only with microfluidic/microscale equipment, but with macroscopic equipment to allow for the easy injection of liquids and extraction of sample droplets, etc. | 08-04-2011 |
20110190147 | MICROARRAY REACTION DEVICE AND METHOD OF USING THE SAME - A microarray reaction device includes a fluid container, a reaction chamber, a first channel connected with the fluid container, a second channel connected with the reaction chamber, and a valve. The valve includes a first and second support unit, respectively including a first and second penetration opening unit, extended through a first and second surface thereof. The first and second penetration opening unit is connected to a second end of the first and second channel, respectively. The second support unit includes a third penetration opening unit extended through a second surface thereof. The first and second surfaces contact each other, such that the first support unit and the second support unit are slidably disposed with each other. The microarray reaction device further includes a storing chamber connected with the third penetration opening unit, and a pump connected to the storing chamber and providing pressure to the storing chamber. | 08-04-2011 |
20110195848 | GENE EXPRESSION AND BREAST CANCER - This invention provides methods and reagents for determining breast cancer patient prognosis and/or diagnosis of tumor aggressiveness, disease-free survival times and reduced patient disease-free survival metrics. | 08-11-2011 |
20110195849 | METHODS AND COMPOSITIONS FOR IDENTIFYING INHIBITORS OF MUTS-alpha OR MUTS-beta INTERACTION WITH MUTL-alpha - Disclosed are methods and kits for screening potential inhibitors of MutSβ by screening agents for the ability to selectively inhibit interaction between MutSβ and MutLα. Also disclosed are kits for performing the methods of the invention. | 08-11-2011 |
20110195850 | COMPACT NANOFABRICATION APPARATUS - An apparatus for use in fabricating structures and depositing materials from tips to surfaces for patterning in direct-write mode, providing ability to travel macroscopic distances and yet provide for nanoscale patterning. Useful in small scale fabrication and nanolithography. The instrument can be compact and used on a laboratory bench or desktop. An apparatus comprising: at least one multi-axis assembly comprising a plurality of nanopositioning stages, at least one pen assembly, wherein the pen assembly and the multi-axis assembly are adapted for delivery of material from the pen assembly to a substrate which is positioned by the multi-axis assembly, at least one viewing assembly, at least one controller. Nanopositioning by piezoelectric methods and devices and motors is particularly useful. The apparatus can include integrated environmental chambers and housings, as well as ink reservoirs for materials to be delivered. The viewing assembly can be a microscope with a long working distance. Particularly useful for fabrication of bioarrays or microarrays. The multi-axis assembly can be a five-axis assembly. Software can facilitate efficient usage. | 08-11-2011 |
20110201507 | SEQUENCING METHODS AND COMPOSITIONS FOR PRENATAL DIAGNOSES - The invention provides methods for determining aneuploidy and/or fetal fraction in maternal samples comprising fetal and maternal cfDNA by massively parallel sequencing. The method comprises a novel protocol for preparing sequencing libraries that unexpectedly improves the quality of library DNA while expediting the process of analysis of samples for prenatal diagnoses. | 08-18-2011 |
20110201508 | Methods of Using Porous Particles - The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries. | 08-18-2011 |
20110201509 | METHOD FOR THE MAPPING OF THE LOCAL AT/GC RATIO ALONG DNA - The invention relates to a method for analysis of the AT/GC ratio of DNA by stretching the DNA in nanochannels and performing melting mapping of the AT/GC ratio along the DNA molecule. | 08-18-2011 |
20110207613 | BIOMARKERS FOR LUPUS - The present invention provides biomarkers valuable in diagnosing or detecting a susceptibility to lupus. The present invention also provides panels of biomarkers valuable in diagnosing or detecting a susceptibility to lupus with good specificity and selectivity. Said biomarkers and panels of biomarkers are also useful in monitoring the progression or regression of lupus, or monitoring the progression to a flare of the disease or transition from a flare into remission or monitoring the efficacy of a therapeutic agent to lupus. | 08-25-2011 |
20110207614 | Polarized Flourescent Nanospheres - The present invention provides partially fluorescent nanoparticles and methods of making and using the partially fluorescent nanoparticle having a nanoparticle with a matrix and a fluorescent dye dispersed in or about the matrix, wherein at least a portion of the fluorescent dye has been anisotropically bleached. | 08-25-2011 |
20110207615 | METHODS FOR MANIPULATING YIELD OF PLANTS AND IDENTIFYING YIELD GENES - Methods for manipulating yield and generation time of plants, especially short day plants such as soybean are provided. The methods comprise manipulating external signals such as long day conditions, short day conditions, growth medium, and nutrient supply. | 08-25-2011 |
20110207616 | SOLID STATE SYNTHESIS HYDROXYL RADICALS FOR HIGH THROUGHPUT STRUCTURE DETERMINATION OF PROTEINS AND NUCLEIC ACIDS BY OXIDATIVE FOOTPRINTING - The present invention generally relates to an apparatus for structural mapping of a macromolecule comprising an amount of persulfide effective to generate hydroxyl radicals upon contact with an aqueous solution. The present invention further relates to methods for structural mapping a macromolecule in an aqueous solution and methods for structural mapping a plurality of macromolecules in parallel, wherein each macromolecule is in a separate aqueous solution. | 08-25-2011 |
20110207617 | SINGLE CELL ANALYSIS BY POLYMERASE CYCLING ASSEMBLY - The invention provides a method of making measurements on individual cells of a population, particularly cells that have identifying nucleic acid sequences, such as lymphoid cells. In one aspect, the invention provides a method of making multiparameter measurements on individual cells of such a population by carrying out a polymerase cycling assembly (PCA) reaction to link their identifying nucleic acid sequences to other cellular nucleic acids of interest. The fusion products of such PCA reaction are then sequenced and tabulated to generate multiparameter data for cells of the population. | 08-25-2011 |
20110212844 | HIGH-THROUGHPUT PLATFORM FOR IN-VIVO SUB-CELLULAR SCREENS ON VERTEBRATE LARVAE - High throughput system for in vivo screens on vertebrate larvae. The system includes a source of vertebrate larvae in a liquid medium and loading tube means for aspirating a larva. A detector assembly is provided to differentiate passage of a larva from bubbles and/or debris. An imaging means is provided for both confocal imaging and wide-field fluorescence imaging of the larva. A laser is provided for optical manipulation of the larva. | 09-01-2011 |
20110212845 | Biomarkers for predicting the sensitivity and response of protein kinase CK2-mediated diseases to CK2 Inhibitors - Disclosed are biomarkers for determining the sensitivity of protein kinase CK2-mediated diseases, such as proliferative and/or inflammatory disorders, to treatment with CK2 inhibitors. These biomarkers can be used to predict or select subjects likely to be responsive to treatment with a CK2 inhibitor, and to treat or monitor subjects undergoing treatment with a CK2 inhibitor. | 09-01-2011 |
20110212846 | Methods and compositions for universal detection of nucleic acids - Provided are methods and compositions for detecting the presence or amount of one or more target nucleic acids in a sample. Methods of the present invention include linking universal nucleic acid segments into a single molecule in a linking reaction dependent on a target nucleic acid of interest. A variety of universal segment linking strategies are provided, including preamplification by polymerase chain reaction, ligation-based strategies, reverse transcription and linear polymerase extension. Linking the universal segments into a single molecule generates a tagged target nucleic acid which is detected in a manner dependent on an intramolecular interaction between one universal segment and a second portion of the tagged target nucleic acid. In certain embodiments, the intramolecular interaction includes the formation of a hairpin having a stem between a universal segment at one end of the tagged target nucleic acid and a second universal segment at the opposite end of the tagged target nucleic acid. A variety of detection formats are provided, including solution-phase and surface-based formats. The methods and compositions are well-suited for highly multiplexed nucleic acid detection, and are applicable for the detection of any target nucleic acid of interest in both research and clinical settings. | 09-01-2011 |
20110212847 | COMPOSITIONS AND METHODS RELATING TO MONITORING ALCOHOL CONSUMPTION AND ALCOHOL ABUSE - Methods and compositions according to embodiments of the present invention are provided that specifically and sensitively detect alcohol consumption and whether alcohol consumption is moderate or high in a subject. Aspects of the present invention relate to assays of panels of proteins for detecting non-consumption, moderate consumption and high consumption of ethanol by a subject. | 09-01-2011 |
20110218114 | NUCLEOTIDE TRIPHOSPHATE WITH AN ELECTROACTIVE LABEL CONJUGATED TO THE GAMMA PHOSPHATE - A nucleotide triphosphate (NTP) participates in a phosphorylation reaction, wherein a phosphate group is transferred from the NTP to a substrate by a kinase. Provision in a kinase reaction of a NTP whose gamma phosphate is conjugated to an electroactive label results in the transfer of the gamma phosphate-electroactive label conjugate from the NTP to the substrate. The electroactive label is an organic moiety such as a quinone or a nitroheterocycle, or is a metallocene such as a ferrocene or a cobaltocene. Upon transfer of the gamma phosphate-electroactive label conjugate to an electrode-bound substrate by a kinase, the phosphorylation event is detected electrochemically by cyclic voltammetry. Phosphorylation can also be detected by mass spectrometry of a substrate carrying the electroactive label-conjugated gamma phosphate. NTP comprising the gamma phosphate-electroactive label conjugate is used in methods of detecting the presence of a kinase in a sample, screening candidate compounds that modulate kinase activity, and in methods of diagnosing a disease associated with a kinase. | 09-08-2011 |
20110224087 | SIMULTANEOUS DETERMINATION OF ANEUPLOIDY AND FETAL FRACTION - The invention provides compositions and methods for simultaneously determining the presence or absence of fetal aneuploidy and the relative amount of fetal nucleic acids in a sample obtained form a pregnant female. The method encompasses the use of sequencing technologies and exploits the occurrence of polymorphisms to provide a streamlined noninvasive process applicable to the practice of prenatal diagnostics. | 09-15-2011 |
20110224088 | Biomarkers for subtypes of cervical cancer - The present invention relates to biomarkers for chemoradioresistant subtypes of cervical cancer. In particular the present invention relates to a method for predicting a predisposition to a chemoradioresistant cervical cancer in a subject, a method for diagnosing a chemoradioresistant cervical cancer in a subject, a method for predicting the likelihood of recurrence of cervical cancer in a cervical cancer patient under treatment, and a method for predicting the prognosis for a patient with a chemoradioresistant cervical cancer. | 09-15-2011 |
20110224089 | GENE EXPRESSION SIGNATURE OF GENOMIC INSTABILITY IN BREAST CANCER - Methods of assessing genomic instability in breast cancer tissue by measuring the expression level of genes CDKN2A, SCYA18, STK15, NXF1, cDNA Dkfzp762M127, p28 KIAA0882, MYB, Human clone 23948, RERG, HNF3A, and ACADSB or a nucleic acid sequence comprising about 90% or greater sequence identity to SEQ ID NO: 21 in breast cancer tissue, an array suitable for use in such methods, and related methods and compositions. | 09-15-2011 |
20110230357 | METHOD FOR DETERMINING THE PRIMARY SITE OF CUP - The invention relates to a method for the classification of cancer using a specific multi-class tumor classifier comprising specific sets of genes for the interpretation of expression data obtained from tumor samples. More specifically, the method of the present invention provides an accurate, reproducible, robust, objective and easy to perform method for determining the primary site of a Cancer of Unknown Primary site (CUP). For this purpose, the method provides that a classifier parameter is determined by comparing an expression profile of a tumor sample with a template profile representative for a particular primary site of a cancer. | 09-22-2011 |
20110230358 | IDENTIFICATION OF POLYMORPHIC SEQUENCES IN MIXTURES OF GENOMIC DNA BY WHOLE GENOME SEQUENCING - The present invention relates to methods comprising whole genome sequencing for identifying polymorphisms in samples comprising mixtures of genomes, and for determining and/or monitoring the presence or absence of disorders associated with the identified polymorphisms. | 09-22-2011 |
20110230359 | LABEL-FREE ON-TARGET PHARMACOLOGY METHODS - Disclosed are methods and machines to determine on-target pharmacology of molecules using label-free biosensor cellular assays and label-free biosensor integrative pharmacology. | 09-22-2011 |
20110230360 | METHODS FOR STRATIFYING AND ANNOTATING CANCER DRUG TREAMENT OPTIONS - Personalized medicine involves the use of a patient's molecular markers to guide treatment regimens for the patient. The scientific literature provides multiple examples of correlations between drug treatment efficacy and the presence or absence of molecular markers in a patient sample. Methods are provided herein that permit efficient dissemination of scientific findings regarding treatment efficacy and molecular markers found in patient tumors to health care providers. | 09-22-2011 |
20110230361 | PROSTATE CANCER BIOMARKERS TO PREDICT RECURRENCE AND METASTATIC POTENTIAL - Described herein are methods for predicting the recurrence, progression, and metastatic potential of a prostate cancer in a subject. For example, the method comprises detecting in a sample from a subject one or more biomarkers selected from the group consisting of FOXO1A, SOX9, CLNS1A, PTGDS, XPO1, LETMD1, RAD23B, ABCC3, APC, CHES1, EDNRA, FRZB, HSPG2, and TMPRSS2_ETV1 FUSION. The method can further comprise detecting in a sample from a subject one or more biomarkers selected from the group consisting of miR-103, miR-339, miR-183, miR-182, miR-136, and miR-221. An increase or decrease in one or more biomarkers as compared to a standard indicates a recurrent, progressive, or metastatic prostate cancer. | 09-22-2011 |
20110237443 | Amelogenin SNP on Chromosome X - Disclosed are methods for gender determination in the intron 1 region of the amelogenin locus and a newly discovered single nucleotide polymorphism (SNP) within the X chromosome of the amelogenin locus which can cause allelic dropout. Also disclosed are kits useful in gender determination. | 09-29-2011 |
20110237444 | METHODS OF MAPPING GENOMIC METHYLATION PATTERNS - The invention relates to sample analysis work flows for increasing the efficiency of experiments. Compositions and methods are described for selectively increase the abundance of methylated nucleic acid over non-methylated nucleic acid, followed by analysis of the nucleic acid to identify methylation sites. | 09-29-2011 |
20110237445 | GENETIC ANALYSIS IN MICROWELLS - Method of genetic analysis of single cell samples comprising the steps of a) providing at least one single cell sample contained in a microplate comprising at least 5 wells/cm | 09-29-2011 |
20110245087 | METHOD FOR OPTIMIZING AND VALIDATING AN ASSAY FOR DETERMINING THE PRESENCE OR ABSENCE OF A MEDICAL CONDITION - The invention relates to a method for validation of an assay for determining the presence or absence of a medical condition, wherein the nucleic acid has been treated such that all unmethylated cytosine bases are converted to uracils. According to the invention, the method comprises: a) measuring the concentration of the nucleic acid in biological samples; b) allotting the samples based on the measured concentration of the nucleic acid in the sample to a first sample group if the concentration of the nucleic acid is below a given threshold value, or to a second sample group if the concentration of the nucleic acid is above the given threshold value; c) performing an assay for determining the methylation status of the nucleic acid in the sample obtaining methylation signals, and d) applying a first algorithm to the value if the sample was allotted to the first sample group, or a second algorithm if the sample was allotted to the second sample group. | 10-06-2011 |
20110245088 | AMIDE-BASED SOLUTION-PHASE DERIVED LIBRARY AND METHOD FOR SCREENING THEREOF - An amide-based library is disclosed in the invention which is prepared via amide bond formation coupling an amine with a carboxylic acid. Also, a method using said library for screening a drug candidate is provided in the present invention. Compounds in the present invention having cytotoxicities are useful for a variety of therapeutic applications. | 10-06-2011 |
20110245089 | LABORATORY CENTRAL CONTROL UNIT METHOD AND SYSTEM - The present disclosure provides a method for coordinating data between a plurality of sample processing systems comprising: providing, using a central control unit, data to a pre-analytical sample processing system identifying a sample undergoing pre-analytical processing, receiving data, at the central control unit, indicating the transfer of the sample to an analytical system, providing, from the central control unit (CCU), data associated with the sample to the analytical system, and receiving a result associated with the sample from the analytical system | 10-06-2011 |
20110245090 | COMPOSITIONS AND METHODS FOR THE TREATMENT OF IMMUNE RELATED DISEASES - The present invention relates to compositions containing novel proteins and methods of using those compositions for the diagnosis and treatment of immune related diseases. | 10-06-2011 |
20110245091 | REACTION PROGRESS ASSAY FOR SCREENING BIOLOGICAL ACTIVITY OF ENZYMES - The present invention relates to assays for measurement activity of enzymes acting to produce products by consuming ATP or dATP substrate. The assays can also be used to identify and screen for substances that modulate the activity of ATP- and dATP-dependent enzymes. | 10-06-2011 |
20110251075 | SYSTEM AND METHOD FOR THE ANALYSIS OF BODILY FLUIDS - A system for the rapid characterization of multi-analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member into which a plurality of cavities may be formed. A series of chemically sensitive particles are, in one embodiment positioned within the cavities. The particles may be configured to produce a signal when a receptor coupled to the particle interacts with the analyte. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized. | 10-13-2011 |
20110251076 | NON-INVASIVE DETECTION OF FETAL GENETIC TRAITS - Blood plasma of pregnant women contains fetal and (generally >90%) maternal circulatory extracellular DNA. Most of said fetal DNA contains ≦500 base pairs, said maternal DNA having a greater size. Separation of circulatory extracellular DNA of <500 base pairs results in separation of fetal from maternal DNA. A fraction of a blood plasma or serum sample of a pregnant woman containing, due to size separation (e.g. by chromatography, density gradient centrifugation or nanotechnological methods), extracellular DNA substantially comprising ≦500 base pairs is useful for non-invasive detection of fetal genetic traits (including the fetal RhD gene in pregnancies at risk for HDN; fetal Y chromosome-specific sequences in pregnancies at risk for X chromosome-linked disorders; chromosomal aberrations; hereditary Mendelian genetic disorders and corresponding genetic markers; and traits decisive for paternity determination) by e.g. PCR, ligand chain reaction or probe hybridization techniques, or nucleic acid arrays. | 10-13-2011 |
20110251077 | Methods and Systems for Diagnosis of Non-Central Nervous System (CNS) Diseases in CNS Samples - The invention features methods, systems, and compositions for diagnosing non-central nervous system (non-CNS) disorders by detecting changes in gene expression in the CNS, e.g., in cerebrospinal fluid, in brain or spinal cord tissue samples, or other bodily fluid samples. | 10-13-2011 |
20110251078 | Methods for Sequencing Individual Nucleic Acids Under Tension - The invention provides apparatuses and methods of use thereof for sequencing nucleic acids subjected to a force, and thus considered under tension. The methods may employ but are not dependent upon incorporation of extrinsically detectably labeled nucleotides. | 10-13-2011 |
20110251079 | HIGH THROUGHPUT NUCLEIC ACID SEQUENCING BY EXPANSION - Nucleic acid sequencing methods and related products are disclosed. Methods for sequencing a target nucleic acid comprise providing a daughter strand produced by a template-directed synthesis, the daughter strand comprising a plurality of subunits coupled in a sequence corresponding to a contiguous nucleotide sequence of all or a portion of the target nucleic acid, wherein the individual subunits comprise a tether, at least one probe or nucleobase residue, and at least one selectively cleavable bond. The selectively cleavable bond(s) is/are cleaved to yield an Xpandomer of a length longer than the plurality of the subunits of the daughter strand, the Xpandomer comprising the tethers and reporter elements for parsing genetic information in a sequence corresponding to the contiguous nucleotide sequence of all or a portion of the target nucleic acid. Reporter elements of the Xpandomer are then detected. Corresponding products, including Xpandomers and oligomeric and monomeric substrate constructs are also disclosed. | 10-13-2011 |
20110251080 | SAMPLE PROCESSING APPARATUS AND METHOD - The invention relates to a sample processing apparatus comprising a holder for a microtiter plate comprising a plurality of microwells, optical measurement unit for measuring optical responses of samples dosed to the microwells, and a computing unit configured to analyze the optical responses in order to detect dosing failures in said plurality of microwells, and if a dosing failure has been detected in one or more of the microwells, to communicate the existence of the dosing failure to a user of the apparatus through signaling means or to store data indicative of the dosing failure to data storage means for further use. In particular, the invention relates to detecting dosing failures in before, during and after a PCR process. | 10-13-2011 |
20110257020 | Biomarker-Based Methods For Formulating Compositions That Improve Skin Quality And Reduce The Visible Signs Of Aging In Skin - In various embodiments, provided are methods for formulating compositions for treating and maintaining the quality of skin, wherein a composition is selected for use in a personal care product based on its demonstrated biological effect to improve skin quality as evidenced by one or more biomarker changes that correlate with improvement as evidenced by one or more objective measurements of skin health. | 10-20-2011 |
20110257021 | USE OF THE MST PROTEIN FOR THE TREATMENT OF A THROMBOEMBOLIC DISORDER - The present invention refers to the use of the Mst protein or a nucleotide sequence coding for the Mst protein for the treatment of a thromboembolic disorder and to a method of screening a modulator of the Mst protein or the nucleotide sequence coding for the Mst protein. | 10-20-2011 |
20110257022 | METHOD FOR ASSESSMENT OF SEVERITY OF LIVER CIRRHOSIS - Disclosed is a method for diagnosing whether a subject suffers from a mild or severe form of liver cirrhosis based on determining the amount of GDF-15 (growth differentiation factor 15), PlGF (placental growth factor), and/or hepatocyte growth factor (HGF) in a sample from the subject and comparing the thus determined amount(s) with a reference amount (reference amounts). The method may further include determining the amount of adiponectin in a sample from the subject, and comparing the amount to a reference amount for adiponectin. Also described is a method to identifying a subject being susceptible to liver transplantation including determining the amount of GDF-15, PlGF, and/or HGF in a sample from the subject and comparing the thus determined amount(s) with a reference amount (reference amounts). | 10-20-2011 |
20110263437 | Analysis of Y-Chromosome STR Markers - The methods and compositions provided herein relate to the discovery of 13 STR are markers found on the human Y chromosome with surprisingly high mutation rates when compared with 173 other Y-STR markers known today, including those that are in common use in forensics. In addition to theoretical expectations based on elevated mutation rates, these 13 rapidly-mutating (RM) Y-STRs proved to be suitable for differentiating between closely related males, as well as between more distant male relatives and much more so than the most-commonly used Y-STRs in forensics. Our new set of RM-Y-STRs is expected to overcome the current dilemma of Y-chromosome analysis in forensic applications from current male lineage identification towards male individual identification in many cases, due to their extraordinary mutation properties as discovered here. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers. | 10-27-2011 |
20110263438 | Diagnosis and complication risk assessment of pancreatic diabetes using procalcitonin - The invention relates to a method for diagnosis and/or diabetic complications and/or risk assessment of pancreatic diabetes, in particular of diabetic sequelae, wherein a determination of the marker procalcitonin (PCT: SEQ ID No. 1) or a partial peptide or fragment thereof or if contained in a marker combination (Panel, Cluster) is carried out on a patient under investigation. The invention further relates to a diagnostic device and a kit for carrying out said method. | 10-27-2011 |
20110263439 | MARKERS ASSOCIATED WITH ALZHEIMER'S DISEASE - This application discloses SNPs capable of predicting increased or decreased risk of developing late onset Alzheimer's disease. | 10-27-2011 |
20110263440 | COLORIMETRIC BIOASSAY FOR PERCHLORATE - The present invention relates to methods, compositions and systems for detecting perchlorate in a sample. Compositions useful for detecting perchlorate in a sample include those comprising a perchlorate reductase, a reductant and an electron shuttle. In an exemplary embodiment, the composition comprises perchlorate reductase from | 10-27-2011 |
20110263441 | COMPOSITIONS, KITS, AND METHODS FOR IDENTIFICATION, ASSESSMENT, PREVENTION, AND THERAPY OF HEPATIC DISORDERS - The invention relates to compositions, kits, and methods for detecting, characterizing, preventing, and treating hepatic disorders such as hepatocellular carcinoma and/or cirrhosis. A variety of informative biomarkers corresponding thereto, are provided, wherein alterations in expression relative to a control is correlated with the presence of a hepatic disorder, likelihood of survival from a hepatic disorder, and likelihood of recurrence of a hepatic disorder. | 10-27-2011 |
20110263442 | METHOD OF USING BIOMARKERS - The present invention provides methods and compositions for predicting patient responses to cancer treatment using the biomarkers: YAP-1, bcl-2, VEGF-c, c-met, and claudin-4. | 10-27-2011 |
20110263443 | DIFFERENTIATING BETWEEN FIBROSIS AND CIRRHOSIS - The present invention is concerned with a method for differentiating between liver fibrosis and liver cirrhosis in a subject based on the determination of growth differentiation factor 15 (GDF-15), hepatocyte growth factor (HGF) and/or endoglin in a sample of a subject and comparing the thus determined amount with a reference amount (reference amounts). Further envisaged by the present invention are kit and a device adapted to carry out the method of the present invention. | 10-27-2011 |
20110269632 | Detection of Cancer through Breath Comprising a Sensor Array Comprising Capped Conductive Nanoparticles - Sensor array for detecting biomarkers for cancer in breath samples. The sensor array is based on 2D films or 3D assemblies of conductive nanoparticles capped with an organic coating wherein the nanoparticles are characterized by a narrow size distribution. Methods of use of the sensor array for discriminating between patterns of volatile organic compounds from healthy individuals and patients with various types of cancer are disclosed. | 11-03-2011 |
20110275524 | METHODS AND SYSTEMS TO DETECT AN ACTIVE PROTEASE IN A SAMPLE - Provided herein are a method and system to detect an active protease in a sample, and related methods and systems to diagnose a condition in an individual, the condition being associated to abnormal protease activity in the individual. | 11-10-2011 |
20110275525 | Human Intestinal Normal Bacterial Flora DNA Chip and Method for Estimating Harmness to Human Body Due to Change of Human Intestinal Normal Bacterial Flora Using DNA Chip - The present invention relates to a DNA chip showing specific responses to a human intestinal normal bacterial flora and a method for estimating harmness to the human bodies due to the change of the human intestinal normal bacterial flora using the DNA chip. | 11-10-2011 |
20110281740 | Methods for Real Time Single Molecule Sequencing - Provided herein are methods and compositions for real time single molecule sequencing of a polymeric molecule, such as a polynucleotide, by isolating the polymeric molecule in a nanofluidic device, subjecting it in situ to a polymerase reaction wherein various components of the polymerase reaction mixture are labeled, and determining the time-sequence of incorporation of monomeric subunits during the polymerization process. | 11-17-2011 |
20110281741 | Method and Apparatus for Rapid Nucleic Acid Sequencing - Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions. | 11-17-2011 |
20110281742 | METHOD FOR IDENTIFYING ELECTROPHILES AND NUCLEOPHILES IN A SAMPLE - A method and device for identifying a molecule in a sample, the molecule comprising an electrophilic or nucleophilic moiety. The method comprises contacting the sample with a plurality of chemosensors, each of the chemosensors comprising a π-conjugated system and a moiety having a nucleophilic property or an electrophilic property; and measuring an electromagnetic property of each of the chemosensors in the sample; whereby the pattern of changes in the electromagnetic properties of the plurality of chemosensors after chemically reacting with the electrophile or nucleophile of the molecule identifies the molecule in said sample. The device comprises a substrate carrying a plurality of chemosensor molecules having at least one predetermined electromagnetic property, the at least one electromagnetic property being changeable by subjecting the chemosensor molecules to a sample containing at least one electrophile or nuclephile, wherein the pattern of change of the electromagnetic property of the plurality of chemosensor molecules allows the device to identify the electrophile or nuclephile in the sample. | 11-17-2011 |
20110281743 | SYSTEMS AND METHODS FOR ISOLATING CELLS IN CELL COLONIES IN CULTURE - Selecting and propagating a cell colony of interest from among a plurality of cell colonies carried on a common substrate in culture is carried out by: (a) selecting a cell colony of interest from among the plurality of cell colonies; (b) isolating a cell subset from the cell colony of interest; (c) analyzing (for example, by a destructive analysis) the cell subset isolated from the cell colony of interest to confirm the presence or absence of a desired feature therein; and then (d) propagating the cell colony of interest when the desired feature is present in the cell subset. A micropallet apparatus may include: (a) a substrate; (b) a plurality of discrete arrays formed on the substrate, each of the arrays comprising a plurality of releasable pallets, and (c) a plurality of gap forming regions, wherein the gap forming regions surround the pallets and separate the pallets from one another. | 11-17-2011 |
20110287947 | Tethered Conformation Capture - Disclosed are methods and systems for determining the three-dimensional structure of chromatin in eukaryotic cells. More specifically, disclosed are methods and systems for obtaining chromatin structural information by surface immobilization, i.e tethering crosslinked protein:DNA complexes and/or ligated DNA complexes to media such as beads, gels, and or matrices during the conformation capture assay. In general, the method includes contacting a cell with a cross-linking reagent to cross-link DNA and protein in the cell; lysing the cell, producing cross-linked protein:DNA complexes by cutting the chromatin using a chemical, physical or enzymatic method, substantially immobilizing the cross-linked protein:DNA complexes, ligating the cross-linked protein:DNA complexes intramolecularly such that the ligated protein:DNA complexes represent structural organization of the chromatin; characterizing the ligated DNA by sequencing or other methods; and identifying any structural organization of the chromatin. The structural organization preferably includes information relating to interacting loci of the chromatin. | 11-24-2011 |
20110287948 | MEASUREMENT OF MATERIAL PROPERTIES AND RELATED METHODS AND COMPOSITIONS BASED ON CYTOADHERENCE - The invention in some aspects relates to methods, devices and compositions for evaluating material properties, such as mechanical and rheological properties of substances, particularly biological substances, such as cells, tissues, and biological fluids. In some aspects, the invention relates to methods, devices and compositions for evaluating material properties of deformable objects, such as cells. In further aspects, the invention relates to methods, devices and compositions for diagnosing and/or characterizing disease based on material properties of biological cells. | 11-24-2011 |
20110287949 | METHODS FOR DETECTING AND TREATING CANCER - The invention generally features compositions and methods for the diagnosis, treatment, and monitoring of neoplasia in a subject, as well as methods of treatment selection. | 11-24-2011 |
20110287950 | Protein fragment complementation assays for the detection of biological or drug interactions - We describe a strategy for designing and implementing protein-fragment complementation assays (PCAs) to detect biomolecular interactions in vivo and in vitro. The design, implementation and broad applications of this strategy are illustrated with a large number of enzymes with particular detail provided for the example of murine dihydrofolate reductase (DHFR). Fusion peptides consisting of N- and C-terminal fragments of murine DHFR fused to GCN4 leucine zipper sequences were coexpressed in | 11-24-2011 |
20110287951 | METHODS AND SYSTEMS FOR PURIFYING, TRANSFERRING, AND/OR MANIPULATING NUCLEIC ACIDS - The disclosure provides methods, systems, and devices for purifying, transferring or manipulating nucleic acids while maintaining the 2D spatial relationship of the nucleic acids as they were present in the original sample having 2D spatial information. | 11-24-2011 |
20110287952 | IRE-1alpha SUBSTRATES - IRE-1α substrates useful for identifying agonists and antagonists of IRE- | 11-24-2011 |
20110294673 | Genetic Variants for Breast Cancer Risk Assessment - The invention pertains to certain genetic variants that have been determined to be susceptibility variants of breast cancer. Methods of disease management, including diagnosing increased susceptibility to breast cancer, methods of predicting response to therapy and methods of predicting prognosis using such variants are described. The invention further relates to kits useful in the methods of the invention. | 12-01-2011 |
20110294674 | MODIFIED RNASE H AND DETECTION OF NUCLEIC ACID AMPLIFICATION - A reversibly modified ‘hot start’ RNAse H enzyme composition is described for the improved CATACLEAVE™ probe detection of nucleic acid sequences in a test sample. A key feature of the enzyme composition is the ability to regulate the catalytic activity of the RNAse H during the course of a reverse transcription-PCR cycle. Thus, RNAse H activity can be initially suppressed to minimize degradation of RNA:DNA primer heteroduplexes prior to reverse transcription. After cDNA synthesis is complete, RNAse H activity is induced to promote the cleavage and fluorescent detection of CATACLEAVE™ probes that anneal to target DNA sequences within the reverse transcriptase-PCR products. The inducible RNAse H enzyme is amenable to high throughput applications requiring one step reverse transcriptase CATACLEAVE™ PCR in a single reaction mix. | 12-01-2011 |
20110294675 | NOVEL COMBINATION OF FLUORESCENT DYES FOR THE DETECTION OF NUCLEIC ACIDS - The present invention relates to combinations of fluorescent dyes used in molecular biology, particularly in multiplex PCR. In particular, the present invention relates to a combination of dyes for amplification reactions, wherein at least four different dyes are used, wherein the first dye is 5-FAM or 6-FAM or a blend thereof, the second dye is selected from the group consisting of DY-530, HEX, CAL Fluor Orange 560 and ATTO 532, the third dye is selected from the group consisting of ATTO 550, DY-555 and DY-556, the fourth dye is selected from the group consisting of ROX, DY-510XL and ATTO 565, and optionally a fifth dye is selected from the group consisting of DY-632 and DY-520XL. | 12-01-2011 |
20110294676 | MONOCHROME MULTIPLEX QUANTITATIVE PCR - Disclosed herein are methods and compositions for determining the copy number of a first target nucleic acid as compared to the copy number of a second target nucleic acid in a single well with a single detection label. For example, disclosed herein are methods and compositions for determining the copy number of a first target nucleic acid as compared to the copy number of a second target nucleic acid by a monochrome multiplex quantitative PCR (MMQPCR) in a single well with a single detection label. | 12-01-2011 |
20110294677 | MICROFLUIDIC SYSTEMS COMPRISING A RUBBER MATERIAL SUBSTRATE - The invention relates to a novel microfluidic system having a substrate based on rubber material having polar side groups which are linked to the rubber polymer backbone via a spacer. By doing so, a transport of water-based fluids such as blood, saliva etc. will occur by capillary forces. | 12-01-2011 |
20110301045 | Composition and Methods Related to Modification of 5-Hydroxymethylcytosine (5-hmC) - The present invention relates generally to the field of molecular biology. More particularly, it concerns methods and compositions for detecting, evaluating, and/or mapping 5-hydroxymethyl-modified cytosine bases within a nucleic acid molecule. | 12-08-2011 |
20110301046 | Methods of Determining Efficacy of Glucocorticoid Treatment of Eosinophilic Esophagitis - The present invention concerns methods useful in diagnosing, identifying and monitoring the progression of eosinophilic esophagitis through measurements of gene products. | 12-08-2011 |
20110301047 | SENSING DEVICE FOR SENSING A FLUID - The invention relates to a sensing device for sensing a fluid. The sensing device comprises an inlet port ( | 12-08-2011 |
20110306505 | X-STR multiplex PCR amplification system - The methods and compositions provided herein relate to the discovery of several new STR alleles and a 20-plex multiplex assay for markers found on the human X chromosome. These X-STR markers have been found when used in a multiplex reaction to provide higher discriminatory power when compared to existing X-STR assay kits available commercially. Embodiments of the present teachings include methods for allelic determination of X-STR markers, amplification primers for the analysis of X-STR markers in a multiplex reaction, allelic ladders for analysis of X-STR markers, and kits for the analysis of X-STR markers. | 12-15-2011 |
20110306506 | ASSAY DEVICE AND METHOD FOR PERFORMING BIOLOGICAL ASSAYS - The invention provides a multiplexed assay device comprising a reaction chamber and several sets of encoded microcarriers | 12-15-2011 |
20110312507 | REACTION DISCOVERY SYSTEM - A novel reaction discovery system that does not depend on DNA duplex formation is provided. The advantages of this system include exploring reactions conditions not possible where DNA hybridization is required. For example, the inventive reaction discovery system allows for reaction conditions using organic solvents, higher temperatures, and water-insoluble reagents, catalysts, and ligands. The invention also provides single-stranded oligonucleotide templates with substrate pairs covalently attached and methods of screening for reaction conditions that result in a direct covalent bond between the substrates. Kits are also provided for practicing this novel reaction discovery system. | 12-22-2011 |
20110312508 | PHYSIOGENOMIC METHOD FOR PREDICTING DIABETES AND METABOLIC SYNDROMES INDUCED BY PSYCHOTROPIC DRUGS - The invention is generally directed to a physiogenomic method for predicting diabetes and metabolic syndromes induced by psychotropic drugs. In one embodiment, the invention relates to the use of genetic variants of marker genes to predict the likelihood that an individual will experience undesirable metabolic side effects as a result of the use of a drug including, but not limited to, psychotropic drugs. The invention also relates to methods predicting the likelihood of diabetes and metabolic syndromes induced by the use of drugs with undesirable metabolic side effects. | 12-22-2011 |
20110312509 | BIOMARKERS DOWNREGULATED IN PROSTATE CANCER - Biomarkers are identified by analyzing gene expression data using support vector machines (SVM), recursive feature elimination (RFE) and/or linear ridge regression classifiers to rank genes according to their ability to separate prostate cancer from normal tissue. Proteins expressed by identified genes are detected in patient samples to screen, predict and monitor prostate cancer. | 12-22-2011 |
20110319276 | NUCLEOTIDES AND OLIGONUCLEOTIDES FOR NUCLEIC ACID SEQUENCING - Embodiments of the invention provide non-natural bifunctional nucleotides having both nuclease resistance and nucleic acid synthesis blocking properties and methods of sequencing nucleic acids that employ non-natural bifunctional nucleic acids. Additional embodiments provide non-natural oligonucleotides and methods for sequencing nucleic acids using the non-natural oligonucleotides. Methods according to embodiments of the invention employ electronic detection and fluorescent detection of nucleic acid sequencing reactions. | 12-29-2011 |
20110319277 | PRPK-TPRKB MODULATORS AND USES THEREOF - The present invention provides methods of identifying an agent that modulates PRPK, TPRKB, OSGEP, LAGE3 and its homologs, PRPK/TPRKB complex, and/or KEOPS complex. The present invention also provides compositions of such agents and methods of treating a disease, disorder, or condition associated with PRPK, TPRKB, OSGEP, LAGE3 and its homologs family (CT antigens), PRPK/TPRKB complex, and/or KEOPS complex using such an agent or composition. | 12-29-2011 |
20120004115 | SYSTEM AND METHOD FOR PURIFICATION AND USE OF INORGANIC PYROPHOSPHATASE FROM AQUIFEX AEOLICUS - The present invention provides a nucleic acid that comprises a nucleic acid of SEQ ID NO: 1 or 3 encoding an Aae pyrophosphatase protein, an enzyme protein of SEQ ID NO: 2 or 4, and methods of sequencing using an isolated Aae pyrophosphatase protein. | 01-05-2012 |
20120004116 | METHODS FOR BIOMARKER IDENTIFICATION AND BIOMARKER FOR NON-SMALL CELL LUNG CANCER - There is provided a method for identifying a biomarker, such as a gene signature, associated with a biological parameter A 6-gene signature for non-small cell lung cancer (NSCLC) is also provided, as well as a method of prognosing or classifying a subject with non-small cell lung cancer into a poor survival group or a good survival group, using said gene signature | 01-05-2012 |
20120004117 | DIAGNOSIS AND TREATMENT OF CANCER USING ANTI-TM4SF20 ANTIBODY - The present invention relates to an antibody binding to a TM4SF20 protein and the diagnosis and treatment of cancer using the antibody. Specifically, the present invention provides an anti-TM4SF20 antibody and a pharmaceutical composition (e.g., an anticancer agent and a diagnostic drug for cancer) comprising the antibody as an active ingredient. | 01-05-2012 |
20120004118 | METHODS FOR THE SUBCLASSIFICATION OF BREAST TUMOURS - Provided is a method for the analysis of breast cancer disorders, comprising determining the genomic methylation status of one or more CpG dinucleotides. Furthermore, a computer program product stored on a computer-readable medium comprising software code adapted to perform the steps of the method when executed on a data-processing apparatus is provided. A device comprising means for supporting a clinician is also provided. | 01-05-2012 |
20120004119 | GENE EXPRESSION MARKERS OF ONCOLYTIC VIRUS SENSITIVITY - A method of predicting an efficacy of an oncolytic virus treatment for a tumor comprises calculating a single-gene predictor score for each of a plurality of genes; calculating a predictor score from the sum of the single-gene predictor scores for the plurality of genes; and predicting, if the predictor score is greater than a predictor score threshold, that the treatment would have efficacy, and if the predictor score is less than a predictor score threshold, that the treatment would lack efficacy. Also, methods of predicting an efficacy of a treatment for a tumor comprise identifying the type and subtype of the tumor, wherein the efficacy of the treatment for the type and subtype of the tumor is known. | 01-05-2012 |
20120004120 | SELF-ENCODING SENSOR WITH MICROSPHERES - Disclosed herein are compositions and methods for combining the output obtained from redundant sensor elements in a sensor array. | 01-05-2012 |
20120004121 | Diagnostic Assay For Source of Inflammation - A method of diagnosing the source of local, acute inflammation has been developed based on the discovery that white cells have different patterns of gene expression, and therefore protein markers, depending on the origin of the inflammation. These differences can be readily accessed by analysis of the white cells obtained at a site to be analyzed, for example, in the synovial fluid of a knee. The analysis, by comparison with the analysis of white cells present in known conditions, can be used to differentiate between inflammation due to bacterial infection, arthritis or gout, for example. The examples demonstrate differential gene expression in cells present in synovial fluid biopsies from patients with confirmed bacterial infection as compared to patients with aseptic loosening or patients with inflammation due to gout. | 01-05-2012 |
20120010088 | GEL HAVING BIOSUBSTANCE FIXED THERETO AND MICROARRAY UTILIZING THE GEL - The present invention provides a biological substance-immobilized gel which comprises a gel containing 2%-7% by mass of N,N-dimethylacrylamide and a biological substance immobilized on and/or in the gel. | 01-12-2012 |
20120010089 | PCR-BASED DETECTION METHOD FOR CHLAMYDIA TRACHOMATIS - A process for designing of PCR-based detection method for | 01-12-2012 |
20120010090 | METHOD OF DIAGNOSING BREAST CANCER - Objective methods for detecting and diagnosing breast cancer (BRC) are described herein. In one embodiment, the diagnostic method involves determining the expression level of a BRC-associated gene that discriminates between BRC cells and normal cells. In another embodiment, the diagnostic method involves determining the expression level of a BRC-associated gene that discriminates among BRC cells, between DCIS and IDC cells. The present invention further provides means for predicting and preventing breast cancer metastasis using BRC-associated genes having unique altered expression patterns in breast cancer cells with lymph-node metastasis. Finally, the present invention provides methods of screening for therapeutic agents useful in the treatment of breast cancer, methods of treating breast cancer and method for vaccinating a subject against breast cancer. | 01-12-2012 |
20120010091 | GENE EXPRESSION ANALYSIS IN SINGLE CELLS - The present invention provides methods and compositions for the analysis of gene expression in single cells or in a plurality of single cells. The invention provides methods for preparing a cDNA library from individual cells by releasing mRNA from each single cell to provide a plurality of individual mRNA samples, synthesizing cDNA from the individual mRNA samples, tagging the individual cDNA, pooling the tagged cDNA samples and amplifying the pooled cDNA samples to generate a cDNA library. The invention also provides a cDNA library produced by the methods described herein. The invention farther provides methods for analyzing gene expression in a plurality of cells by preparing a cDNA library as described herein and sequencing the library. | 01-12-2012 |
20120015827 | METHOD FOR THERAPY PREDICTION IN TUMORS HAVING IRREGULARITIES IN THE EXPRESSION OF AT LEAST ONE VEGF LIGAND AND/OR AT LEAST ONE ERBB-RECEPTOR - The present invention is related to a method for predicting a clinical response of a patient suffering from or at risk of developing a neoplastic disease towards a given mode of treatment, said method comprising the steps of: a) obtaining a biological sample from said patient; b) determining, on a non protein basis, the expression level of at least one gene encoding for a ligand from the Vascular endothelial growth factor (VEGF) family and of and of at least one gene encoding for a receptor from the ErbB-family, or a gene co-expressed therewith, in said sample, c) comparing the pattern of expression levels determined in (b) with one or several reference pattern (s) of expression levels; and d) predicting therapeutic success for said given mode of treatment in said patient or implementing therapeutic regimen targeting the signalling pathway of said ligand and/or receptor is related to in said patient from the outcome of the comparison in step (c). | 01-19-2012 |
20120015828 | SAMPLE ANALYSIS CHIP, SAMPLE ANALYZER USING SAMPLE ANALYSIS CHIP, SAMPLE ANALYSIS METHOD, AND METHOD OF PRODUCING SAMPLE ANALYSIS CHIP - A low cost sample analysis chip in which liquid is supplied to wells formed on the chip so as to carry out reaction by a simple liquid supply method with no variation in the amount of liquid in each well, the sample analysis chip including a plurality of wells and a flow passage leading to the respective wells, wherein the flow passage includes a main flow passage which supplies liquid to each well, and the sample analysis chip has the main flow passage provided closer to the rotation center side than the well, and is formed so as to have one peak between neighboring wells in the direction of rotation center. | 01-19-2012 |
20120015829 | Adaptive Immunity Profiling and Methods for Generation of Monoclonal Antibodies - Methods are provided for producing monoclonal antibody candidates using adaptive immunity profiling. In some aspects, the method provides for the use of massively parallel signature sequencing. | 01-19-2012 |
20120015830 | MICRORNA PROFILES FOR EVALUATING MULTIPLE SCLEROSIS - The present invention provides methods, systems, and kits for evaluating multiple sclerosis (MS) in a patient. Particularly, the invention provides convenient miRNA-based tests for evaluating a patient for MS, including for diagnosing MS, for excluding MS as a diagnosis, for determining the presence of disease activity associated with MS, and for monitoring the course of disease or efficacy of treatment for MS. | 01-19-2012 |
20120015831 | METHOD FOR DELIVERING NUCLEIC ACID INTO EUKARYOTIC GENOMES - The present invention relates to genetic engineering and especially to the use of DNA transposition complex of bacteriophage Mu. In particular, the invention provides a gene transfer system for eukaryotic cells, wherein in vitro assembled Mu transposition complexes are introduced into a target cell and subsequently transposition into a cellular nucleic acid occurs. The invention further provides a kit for producing insertional mutations into the genomes of eukaryotic cells. The kit can be used, e.g., to generate insertional mutant libraries. | 01-19-2012 |
20120015832 | METHODS, PANELS OF IDENTIFICATION MARKERS, AND KITS FOR IDENTIFYING FORENSIC SAMPLES - Methods for identifying forensic samples using panels of markers and gene expression profiling, including without limitation, mRNA profiling, miRNA profiling, or both, are disclosed. Panels of markers for identifying certain tissue samples and certain body fluid samples are also disclosed. Kits for expediting performance of certain of the disclosed methods are provided. | 01-19-2012 |
20120015833 | METHOD AND APPARATUS FOR DETECTING MOLECULES - The present invention is directed to a method of detecting a molecule attached to a surface comprising detecting a bead attached to said molecule. The method may be used for the detection of single molecules and the surface shape may be adapted to receive beads. The invention further encompasses an apparatus for detecting the presence of a molecule having a bead attached thereto comprising a surface which is provided with a means for detecting a bead wherein the shape of said surface is configured to receive and retain a bead. Kits comprising the apparatus are provided and a use of the apparatus for detecting a bead or a molecule attached thereto is contemplated. | 01-19-2012 |
20120021921 | PROCESS AND METHOD FOR MONITORING GASTROINTESTINAL MICROBIOTA - Disclosed are methods for monitoring the gastrointestinal tract of the human gastrointestinal system. The method includes: 1) grouping microbes into specific operational taxonomic units (OTU); 2) using oligonucleotide probes and PCR primers to detect and quantify specific microbes (bacteria, fungi/yeast, protozoans and parasitic worms) in human fecal material. The inventions also discloses a kit that includes: a DNA isolation step; 2) accumulation of specific operational taxonomic units (OTU); 3) identification and quantifying of sequences internal to the OTU; 4) reporting changes the indigenous population of the human gastrointestinal system. | 01-26-2012 |
20120021922 | TRANSLATION-COUPLING SYSTEMS - Disclosed are systems and methods for coupling translation of a target gene to a detectable response gene. A version of the invention includes a translation-coupling cassette. The translation-coupling cassette includes a target gene, a response gene, a response-gene translation control element, and a secondary structure-forming sequence that reversibly forms a secondary structure masking the response-gene translation control element. Masking of the response-gene translation control element inhibits translation of the response gene. Full translation of the target gene results in unfolding of the secondary structure and consequent translation of the response gene. Translation of the target gene is determined by detecting presence of the response-gene protein product. The invention further includes RNA transcripts of the translation-coupling cassettes, vectors comprising the translation-coupling cassettes, hosts comprising the translation-coupling cassettes, methods of using the translation-coupling cassettes, and gene products produced with the translation-coupling cassettes. | 01-26-2012 |
20120021923 | METHODS AND SYSTEMS TO DIAGNOSE A CONDITION IN AN INDIVIDUAL - Provided herein are a method and system to detect an active protease in a sample, and related methods and systems to diagnose a condition in an individual, the condition being associated to abnormal protease activity in the individual. | 01-26-2012 |
20120021924 | DETECTION AND MODULATION OF CYTOCHROME C ACETYLATION - The invention relates to detection and modulation of cytochrome c acetylation. The invention has diagnostic and therapeutic applications for neurodegenerative disorders and cancer. | 01-26-2012 |
20120021925 | DIAGNOSTIC ASSAYS FOR PROSTATE CANCER USING PSP94 AND PSA BIOMARKERS - The application refers to a method for diagnosing prostate cancer through the measurement of the combination of PSP94 (β-microseminoprotein) and F/T PSA biomarkers, the method using urine samples to measure PSP94 and serum samples to analyse F/T PSA. In one embodiment, the PSP94 is standardized to creatinine. Also based on the measurement of PSP94 and F/T PSA are provided methods and kits for (a) diagnosing aggressive prostate cancer; (b) differential diagnosis; and (c) diagnosing the progression of prostate cancer. | 01-26-2012 |
20120021926 | FLUORESCENCE RESONANCE ENERGY TRANSFER ASSAYS FOR SARCO/ENDOPLASMIC RETICULUM CALCIUM ATPASE AND PHOSPHOLAMBAN - Provided herein are methods for identifying molecules capable of modulating SERCA, the SERCA-PLB complex or the microenvironment of the complex. An exemplary assay provided herein is fluorescence resonance energy transfer (FRET). Also provided herein are FRET assays that are optimized for high-throughput screening (HTS) for identifying small molecules that modulate SERCA or the SERCA-PLB complex. Further provided are kits for carrying out said methods for identifying molecules. | 01-26-2012 |
20120021927 | Methods for Determining a Prognosis for Survival for a Patient with Breast Cancer - A method for determining a prognosis for survival for a patient with breast cancer is described. Also described is method for monitoring the effectiveness of a course of treatment for a patient with breast cancer, and the use of such a method in a kit. A kit determining the level of RINF is also described. | 01-26-2012 |
20120021928 | GENETIC RISK ASSESSMENT FOR SHAR-PEI FEVER - The present invention provides genetic tests for the copy number or a duplication of an upstream element of the Hyaluronic Acid Synthase 2 (HAS2) gene on chromosome 13 in Shar-Pei dogs to assess risk for a Shar-Pei dog to develop Shar-Pei fever. As Shar-Pei fever is a serious disease, and its chronic state involves a high risk of developing systemic amyloidosis and kidney failure, a genetic test for the copy number of the duplication will be useful in Shar-Pei breeding. Also provided are probes and primer pairs for use in these methods. | 01-26-2012 |
20120021929 | USE OF DPPIV/SEPRASE AS A MARKER FOR CANCER - Described is a method aiding in the assessment of the presence of cancer. The method uses the soluble DPPIV/seprase protein complex (DPPIV/seprase) as a universal marker of different cancer types. Measurement of DPPIV/seprase complex can, e.g., be used in the early detection or diagnosis of cancer or in the surveillance of patients who undergo surgery. | 01-26-2012 |
20120028817 | Methods and Kits for Genome-Wide Methylation of GpC Sites and Genome-Wide Determination of Chromatin Structure - Disclosed are methods and kits for genome-wide methylation of GpC sites and for genome-wide chromatin structural determination. Specifically, the methods and kits of the present invention make possible the simultaneous determination of endogenous DNA methylation state and chromatin architecture across the entire genome. | 02-02-2012 |
20120028818 | MICRO FLUIDIC STRUCTURES - A micro fluidic system includes a substrate, and, provided on said substrate, at least one flow path interconnecting with functional means in which liquid samples can be treated by desired procedures. The flow paths are laid out to form a pattern for the transport of liquid samples to and from said functional means. These flow paths comprise a plurality of micro posts protruding upwards from said substrate, the spacing between the micro posts being small enough to induce a capillary action in a liquid sample applied anywhere within any of said flow paths, so as to force said liquid to move from where said liquid sample was applied. | 02-02-2012 |
20120028819 | METHOD FOR THE DETECTION AND CHARACTERIZATION OF A TOXINOGENIC CLOSTRIDIUM DIFFICILE STRAIN - The invention relates to a method for the detection and characterization of a toxinogenic | 02-02-2012 |
20120035061 | OPTICAL IMAGING BASED ON VISCOELASTIC FOCUSING - An apparatus arranged for examining particles, comprising: a cartridge having at least one microchannel, a viscoelastic fluid flowing in the microchannel, the fluid comprising a suspension of particles, thereby effecting alignment of the particles in at least one-dimensional array parallel to the fluid flow, and an optical magnifying means generating an image of the particles in the microchannel. | 02-09-2012 |
20120035062 | ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS - A method for sequencing a polynucleotide strand by using sequencing-by-synthesis techniques. To address the problem of incomplete extension (IE) and/or carry forward (CF) errors that can occur in sequencing-by-synthesis reactions, an alternative flow ordering of dNTPs is used. In contrast to conventional flow orderings, the dNTPs are flowed in an ordering that is not a continuous repeat of an ordering of the four different dNTPs. This alternate flow ordering may reduce the loss of phasic synchrony in the population of template polynucleotide strands that result from IE and/or CF errors. | 02-09-2012 |
20120035063 | Use of Chemically Patterned Substrate for Liquid Handling, Chemical and Biological Reactions - Methods and devices for adding liquids to and washing a microfluidic element array are disclosed. The method and devices feature a microfluidic plate holder with a sloped wall for improved draining of liquid, a machine readable/writable identifier, plate leveling systems, liquid filling systems, a hydrophilic-liquid coating, and an automated washing station. | 02-09-2012 |
20120035064 | POLYMORPH, POLYMORPH SCREENING SYSTEM, AND POLYMORPH PREPARING AND SCREENING METHOD - A polymorph, a polymorph screening system, and a polymorph preparing and screening method are disclosed. The polymorph preparing and screening method includes the following steps: providing a plurality of substrates of different materials; causing an organic material to grow crystals on the plurality of substrates through solution-cooling crystallization process, so that a plurality of polymorphs with different characteristics are prepared; and screening the plurality of polymorphs prepared in the previous step to obtain polymorphs with desired characteristics. | 02-09-2012 |
20120040843 | CENTRIFUGAL CAPTURE SYSTEM - A particle capture system that can be used in the context of a lab-on-a-chip platform for particle- and cell-based assays is described. The system comprises a capture chamber comprising a plurality of capture sites, the capture sites defining a capture area configured to receive individual particles travelling within the capture chamber. By rotating the chamber, the individual particles are biased towards the capture sites where they may be captured. | 02-16-2012 |
20120040844 | Methods and Apparatus for Measuring Analytes Using Large Scale FET Arrays - Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis. | 02-16-2012 |
20120040845 | ADVANCED PATHOGEN TESTING AND CARCASS-CERTIFICATION METHODS FOR SLAUGHTER OPERATIONS - The invention provides methods for certification of carcasses, and for detecting a contaminated carcass and preventing its movement into or across a production area. The inventive methods comprise obtaining, early in the production process (pre-fabrication), a test sample from at least one test location of at least one split-portion of each carcass, wherein the test samples are obtained prior to or during chilling of the respective split portions, before entry thereof in the production chain. Composite test samples are assayed for pathogens or microbes, whereby certification is afforded, or whereby entry of the chilled split-carcass-Lot into the production area is precluded if the corresponding composite-Lot test sample is contaminated. Methods for remedial reconditioning of contaminated split-carcasses are provided, wherein essentially 100% of the carcasses are targeted to the production line. The inventive methods provide substantial public health benefit, and are efficient and economical to implement. | 02-16-2012 |
20120040846 | Methods of Detecting Diseases or Conditions Using Phagocytic Cells - This invention provides methods of using phagocytic cells in the diagnosis, prognosis, or monitoring of diseases or conditions. The invention also provides methods of using phagocytic cells to identify markers of diseases or conditions. | 02-16-2012 |
20120058900 | HARDWARE ARCHITECTURE OF ANALYZERS - An analytical apparatus and a method for isolating and analyzing at least one analyte are described, comprising at least one module for receiving and dispensing a sample to be analyzed, at least one module for isolating said analyte to be analyzed, at least one module for analyzing said analyte, wherein said modules (i) to (iii) are arranged along an axis. | 03-08-2012 |
20120058901 | ASSAY FOR THE DETECTION OF RECURRENCE IN BREAST CANCER USING THE NOVEL TUMOR SUPPRESSOR DEAR1 - Certain aspects of the present invention relate to new diagnostic and prognostic methods involving DEAR1, a gene is located on the short arm of human chromosome 1. Specifically, analysis of expression or structure of this gene for prognosis or recurrence risk assessment is disclosed. | 03-08-2012 |
20120058902 | METHOD OF MEASURING ADAPTIVE IMMUNITY - Compositions and methods for measuring adaptive immune receptor (T cell receptor and immunoglobulin) diversity are described, and find uses for assessing immunocompetence and other purposes. Means are provided for assessing the effects of diseases or conditions that compromise the immune system and of therapies aimed to reconstitute it. Lymphoid (B- and T-cell) adaptive immune receptor diversity is quantified by calculating the number of uniquely rearranged, CDR3-containing immunoglobulin (Ig) or T-cell receptor (TCR) variable region-encoding genes from sample cells such as blood cells. | 03-08-2012 |
20120058903 | Gel Microdrop Composition and Method of Using the Same - A gel microdrop composition is provided. In certain embodiments, the gel microdrop composition contains a polymer matrix, an effector particle that releases an effector molecule into the polymer matrix, a first reporter particle that emits a first optically detectable signal and a second reporter particle that emits a second optically detectable signal that is distinguishable from the first optically detectable signal, where the effector particle and said first and second reporter particles are encapsulated by the polymer matrix. Methods of screening that employ the gel microdrop composition and methods of making the gel microdrop composition are also disclosed. | 03-08-2012 |
20120058904 | SPECIES-SPECIFIC PRIMER SETS AND IDENTIFICATION OF SPECIES-SPECIFIC DNA SEQUENCES USING GENOME FRAGMENT ENRICHMENT - Targeted sequencing of genetic regions that differ between two DNA preparations uses genomic fragment enrichment. This method can be used to study genetic variation among closely related species and microbial communities, particularly for identifying sources of fecal pollution. | 03-08-2012 |
20120065082 | SINGLE-CELL MICROCHAMBER ARRAY - An apparatus for analyzing individual cell composition in a heterogeneous cell population may include, in one embodiment, a deposition plate having an array of microwells disposed therein, and a cover plate substantially overlying the deposition plate. A pair of electrodes may be associated with one or more of the microwells, and may be configured to generate an electric field within the associated microwell. | 03-15-2012 |
20120065083 | METHOD AND APPARATUS FOR PREDICTING SUSCEPTIBILITY TO A DEVELOPMENTAL DISORDER - The present invention provides a method of screening a subject for mutations in the TRAPPC9 gene that are associated with developmental disabilities. The present invention also provides proteins that are associated with developmental disabilities including a truncation of NIBP. Also provided are nucleotide sequences encoding such proteins and methods of screening subjects to identify nucleotide sequences or proteins associated with developmental disabilities. | 03-15-2012 |
20120065084 | METHODS AND TOOLS FOR PREDICTING THE EFFICIENCY OF ANTHRACYCLINES IN CANCER - A gene set, kit and method predict the efficiency of anthracyclines-based treatment of breast cancer. | 03-15-2012 |
20120071332 | Method for Quantification of Small RNA Species - The present invention relates to a method for amplification and quantification of microRNA molecules using quantitative reverse transcription polymerase chain reaction (qRT-PCR) technology. The method comprise the steps of (a) producing cDNA molecules complementary to microRNAs in a sample using polyadenylation and primer extension by reverse transcription, and (b) amplification and quantification of the cDNAs by qPCR using microRNA specific primer sets of forward and reverse primers containing LNA monomers. | 03-22-2012 |
20120077684 | Methods and Compositions Including Diagnostic Kits For The Detection In Samples Of Methicillin-Resistant Staphylococcus Aureus - The present invention provides methods, compositions and diagnostic kits for the detection of | 03-29-2012 |
20120077685 | INTRATUMORAL GENE EXPRESSION LEVELS OF TP AND VEGF ARE ASSOCIATED WITH CLINICAL OUTCOMES IN RECTAL CANCER PATIENTS TREATED WITH 5-FLUOROURACIL AND PELVIC RADIATION - The disclosure provides compositions and methods for identifying a cancer patient suitable or not suitable for a therapy that includes administration of a pyrimidine antimetabolite such as 5-fluorouracil. After determining if a patient is likely to be successfully treated, the disclosure also provides methods for treating these patients. | 03-29-2012 |
20120088678 | METHOD FOR PREDICTION OF RESPONSE TO RHEUMATOID ARTHRITIS THERAPEUTICS - The present invention is based in part on the identification of a signature marker profile of immune variables to diagnose an immune mediated disease or for prediction of response to an immune mediated disease therapeutic agent. Additionally, the present invention provides methods for the prediction of response to an immune mediated disease therapeutic agent. | 04-12-2012 |
20120088679 | Method for Monitoring Cell Culture - The present invention is directed to a method of monitoring the physiological state of a cell cultivation. Several parameters such as cell viability, growth, metabolic profile, and productivity may be monitored to establish a metabolic fingerprint or metabolomic profile of a cell culture. | 04-12-2012 |
20120094850 | METHOD FOR DETERMINING THE ORIGIN OF A SAMPLE - A method for determining the nature of a sample is provided, wherein the presence or absence of at least one marker small non-coding RNA in the sample is detected. Suitable marker small non-coding RNAs for different samples such as blood, saliva, semen and vaginal secretions are provided. Also provided are suitable kits and methods for identifying marker small-non coding RNAs. | 04-19-2012 |
20120094851 | Multi-through Hole Testing Plate for High Throughput Screening - A method for holding samples for analysis and an apparatus thereof includes a testing plate with a pair of opposing surfaces and a plurality of holes. Each of the holes extends from one of the opposing surfaces to the other one of the opposing surfaces. The holes are arranged in groups, where each group has at least two rows and two columns of holes. The groups are arranged in sets, where each set has at least two rows and two columns of groups. To analyze samples, at least one of the opposing surfaces of the testing plate is immersed in a solution to be analyzed. A portion of the solution enters openings for each of the holes in the immersed opposing surface. Once the holes are filled with solution, the testing plate is removed and is held above a supporting surface. Surface tension holds the solution in each of the holes. The solution in one or more of the holes is then analyzed and the solution in one of these holes is identified for further study. The location of the identified solution is marked based upon its location within a particular set and group of holes. | 04-19-2012 |
20120100998 | METHOD OF DETECTING CANCER USING DELTA-CATENIN - The present invention provides a method for detecting or screening for the presence of cancer in a subject. The method comprises obtaining, providing or collecting a tissue or fluid sample (such as a urine sample) from said subject, and then determining the presence or absence of delta-catenin in said sample, or increased levels of delta-catenin in said sample as compared to a normal or control subject. The presence of delta-catenin in said sample, or increased levels of delta-catenin in said sample, indicating said subject is afflicted with or at least at risk of developing cancer. | 04-26-2012 |
20120100999 | PROGNOSTIC GENE EXPRESSION SIGNATURE FOR SQUAMOUS CELL CARCINOMA OF THE LUNG - Provided is a gene expression signature consisting of 12 biomarkers for use in prognosing or classifying a subject with lung squamous cell carcinoma into a poor survival group or a good survival group. The 12-gene signature specific for squamous cell carcinoma consists of the biomarkers RPL22, VEGFA, G0S2, NES, TNFRSF25, DKFZP586P0123, COL8A2, ZNF3, PJPK5, RNFT2, ARHGEF12 and PTPN20A. | 04-26-2012 |
20120108445 | VEGF AND VEGFR1 GENE EXPRESSION USEFUL FOR CANCER PROGNOSIS - The invention provides compositions and methods for determining the likelihood of tumor recurrence of adjuvant cancer patients following surgical resection. | 05-03-2012 |
20120108446 | 4-miRNA SIGNATURE FOR PREDICTING CLEAR CELL RENAL CELL CARCINOMA METASTASIS AND PROGNOSIS - An miRNA expression signature comprising a set of one or more miRNAs associated with metastatic cancer is provided. In one embodiment, the expression signature is selected from the group consisting of miR-10b, miR-139-5p, miR-130b and miR-199b-5p. In some aspects, miR-199b-5p and miR-130b are overexpressed in metastatic cancer; and miR-10b and miR-139-5p are downregulated in metastatic cancer. Such an expression signature may be used in methods for predicting metastasis, risk for developing metastasis or a prognosis in a cancer. In another embodiment, a method for establishing such a cancer miRNA expression signature is provided. | 05-03-2012 |
20120108447 | METHODS OF PREDICTING CLINICAL OUTCOME IN MALIGNANT MELANOMA - Malignant melanoma is the most aggressive type of skin cancer and currently accounts for more than 160.000 new cancer cases worldwide every year. Incidence of malignant melanoma is rapidly increasing—with a doubling rate of 10-20 years and a death rate of 11%. The inventors of the present application found genes that predict overall survival of patients with malignant melanoma and that contain prognostic information not comprised in the conventional histopathological and clinical criteria. Accordingly, the present invention relates to the field of oncology, more particularly to methods of predicting clinical outcome in malignant melanoma as defined in the claims. | 05-03-2012 |
20120122704 | Differential multiplexing with pattern recognition - This novel form of multiplexing allows the user to probe for multiple targets and simultaneously identify a specific target. An example of solutions provided here comprises: providing one or more assay mixes for a number of targets (the number of assay mixes is less than the number of targets); providing a number of reference patterns (each of the reference patterns is associated with one of the targets); contacting each of a number of aliquots with one of the assay mixes; generating a result pattern, based on positive or negative results; and selecting the reference pattern most similar to the result pattern, to thereby detect the target. Solutions provided here, which we term differential multiplexing with pattern recognition, may involve molecular or immunological techniques to identify one of many indicators of drug use, illness, disease, or medical condition. For example, we provide a simple method for identifying targets using combinations of real-time PCR reagent mixes and only one reporter. This is an effective and inexpensive method that can be used to identify targets with minimal reagents and supplies. | 05-17-2012 |
20120122705 | FLOW-THROUGH HIGH HYDROSTATIC PRESSURE MICROFLUIDIC SAMPLE PREPARATION DEVICE AND RELATED METHODS THEREFOR - Described herein is a sample preparation device including a sample delivery source, an inline means of transferring the sample from the sample source into a deformable channel within a pressure vessel, and out of the channel into downstream analysis components, a deformable channel disposed within the pressure vessel, the deformable channel having an inlet end and an outlet end fluidly connectable to high pressure valves and a means to measure the fluid pressure within the deformable channel, an external source of a controlled pressurized fluid fluidly connectable to the pressure vessel and a controller system that monitors and controls the sample fluid pressure by control of the external pressure vessel fluid. | 05-17-2012 |
20120122706 | ARRAY USING COLOR REACTION OF CHEMICAL COMPOUNDS - Disclosed herein is an array, including a plurality of sensing units, each of which contains a mixture of porphyrin compounds, and which are arranged in a predetermined pattern. The array is advantageous in that it is possible to qualitatively analyze various mixture samples without performing complicated processes of separation and analysis. | 05-17-2012 |
20120122707 | HIGH THROUGHPUT APPROACH TO FORMULATION USING PRE-DESIGNED FORMULATION PLATES - System and methods provide formulation using pre-designed formulation plates for rapidly formulating proteins and peptides, wherein proteins are added to a plurality of wells in the plate; means for stressing one or more protein formulations; and means for analyzing samples of the protein formulations. | 05-17-2012 |
20120122708 | LIBRARIES OF N-(2-OXO-1-PHENYLPIPERIDIN-3-YL)SULFONAMIDES FOR THE IDENTIFICATION OF BIOLOGICAL AND PHARMACOLOGICAL ACTIVITY - New compounds are continually sought after for the treatment and prevention of disorders. The invention relates to N-(2-oxo-1-phenylpiperidin-3-yl)sulfonamides which can be biologically and pharmacologically traced, in order to be used in the search for, and identification of, new lead compounds that can modulate the functional activity of a biological target. | 05-17-2012 |
20120122709 | LIBRARIES OF 1-(SULFONYL)-N-PHENYLPYRROLIDINE-2-CARBOXAMIDES FOR THE IDENTIFICATION OF BIOLOGICAL AND PHARMACOLOGICAL ACTIVITY - New compounds are continually sought after for the treatment and prevention of disorders. The invention relates to 1-(sulfonyl)-N-phenylpyrrolidine-2-carboxamides which can be biologically and pharmacologically traced, in order to be used in the search for, and identification of, new lead compounds that can modulate the functional activity of a biological target. | 05-17-2012 |
20120122710 | LIBRARIES OF N-SUBSTITUTED-N-PHENYLETHYLSULFONAMIDES FOR THE IDENTIFICATION OF BIOLOGICAL AND PHARMACOLOGICAL ACTIVITY - New compounds are continually sought after for the treatment and prevention of disorders. The invention relates to N-substituted-N-phenylethylsulfonamides which can be biologically and pharmacologically traced, in order to be used in the search for, and identification of, new lead compounds that can modulate the functional activity of a biological target. | 05-17-2012 |
20120122711 | SCREENING OF BIOPOLYMERS - Described herein are inventive compositions and methods relating to sampling of biopolymers and, in particular, to fractional sampling of biopolymers. In one aspect, embodiments are generally related to unique biopolymer species where a fraction of each biopolymer species contains a cleavable linker. The biopolymer species may, in some embodiments, be attached to a surface. For example, the biopolymer species may be attached to beads. In some embodiments, a portion of a unique biopolymer species may be sampled by cleaving the cleavable linker. In some cases, the sample may be analyzed to determine the sequence of the biopolymer. | 05-17-2012 |
20120129705 | DIAGNOSTIC MARKERS FOR DETERMINING THE PREDISPOSITION TO THE DEVELOPMENT OF CERVICAL CANCER AND OLIGONUCLEOTIDES USED FOR THE DETERMINATION - The present invention relates to a method for predisposition prediction of a subject to the development of cervical cancer and/or cancer precursors in an infection with the human papilloma virus (HPV) and/or for the detection of a persistent HPV infection, the method comprising the steps of obtaining a sample from the subject; and detecting at least one of the diagnostic markers or fragments thereof listed in Table 1 in the sample obtained from the subject. | 05-24-2012 |
20120129706 | Method of Assessing Soil Quality and Health - The present invention relates to a method of assessing the soil quality and health of mined and un-mined lands. The present invention further relates to a method of assessing ecosystem health and soil restoration efforts. The method employs a multi-prong approach combining DNA fingerprinting and in-depth pyrosequencing of soil microorganisms that are responsible for maintaining soil fertility and productivity. The method incorporates assessing the indigenous bacterial community structure data derived from PCR-DGGE analysis and 165 rRNA gene clone libraries, and then further identification of those microorganisms that are known to promote soil health by soil metagenome analysis. Disparity between un-mined and post-mined soils are comprehensively studied via multivariate statistical analyses on the polyphasic dataset. | 05-24-2012 |
20120135872 | METHODS OF FETAL ABNORMALITY DETECTION - Methods and kits for selectively enriching non-random polynucleotide sequences are provided. Methods and kits for generating libraries of sequences are provided. Methods of using selectively enriched non-random polynucleotide sequences for detection of fetal aneuploidy are provided. | 05-31-2012 |
20120142541 | Structured Peptide Processing - The invention relates to a method for modifying one or more peptide ligands, comprising polypeptides covalently linked to a molecular scaffold at two or more amino acid residues, comprising the steps of providing one or more peptide ligands, wherein the polypeptide comprises two or more reactive groups which form a covalent linkage to the molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; exposing the peptide ligands to one or more proteases; and sorting the ligands according to the extent of proteolytic cleavage. | 06-07-2012 |
20120142542 | Metabolite Biomarkers for the Detection of Esophageal Cancer Using NMR - Methods for the detection and screening of esophageal adenocarcinoma (EAC) patients and for the monitoring of EAC treatment using a panel or panels of small molecule metabolite biomarkers are disclosed. In other aspects, methods for detection and screening for the progression of high-risk conditions (BE and HOD) to EAC and to monitoring treatment using a panel or panels of small molecule metabolite biomarkers are disclosed. The biomarkers are sensitive and specific for the detection of EAC, and can also be used to classify Barrett's esophagus (BE) and high-grade dysplasia (HOD), which are widely regarded as precursors of EAC. | 06-07-2012 |
20120142543 | Methods to assess treatment outcomes in Reward Deficiency Syndrome (RDS)behaviors utilizing expression profiling - The present invention relates to methods to objectively assess treatment outcomes in Reward Deficiency Syndrome (RDS) behaviors by obtaining expression profiles (e.g., mRNA expression and/or protein expression profiles) for one or more genes at two or more different time points, for example, before and after treating a subject known to have or suspected of having an RDS affliction. Analysis, for example, of mRNA and/or protein expression levels and/or patterns can be conducted before admission to a treatment facility, followed by testing at one or more various designated times during and after a subject's treatment. Such methods may also be combined with other tests, and can be used in diagnosis and treatment of RDS and RDS behaviors, including drug and/or alcohol abuse and addiction, overeating, gambling, sexual addiction, etc. | 06-07-2012 |
20120142544 | DIAGNOSTIC TRANSCRIPTOMIC BIOMARKERS IN INFLAMMATORY CARDIOMYOPATHIES - Molecular signatures that function as very sensitive diagnostic biomarker for myocarditis, heart disease and disorders thereof, are identified. | 06-07-2012 |
20120149586 | METHODS OF PREDICTING THE RISK OF RECURRENCE OF CANCER - Disclosed herein are methods of predicting the risk of recurrence of cancer on the basis of the expression of microRNA of the miR-520 family. | 06-14-2012 |
20120149587 | METHOD FOR DETECTING NUCLEATED CELLS - The invention provides methods to detect or quantify cells such as nucleated cells in a sample such as a physiological sample, which employ magnetic substrates and subjects the sample and the magnetic substrate to forms of energy so as to induce aggregate formation. | 06-14-2012 |
20120149588 | Phased Genome Sequencing - The present disclosure provides methods for determining phased nucleic acid sequence for a single chromosome of interest and/or a single chromosomal fragment of interest. The present disclosure also provides methods for determining phased nucleic acid sequence for a plurality of single chromosomes of interest and/or a plurality of single chromosomal fragments of interest. The plurality of single chromosomes of interest may be of one or more chromosome types. The present disclosure also provides a method for isolating a plurality of chromosomal fragments of a specified size range, where the chromosomal fragments are from one or more specified regions of the genome. The plurality of chromosomal fragments may be separated into single chromosomal fragments and sequenced to provide phased nucleic acid sequence for the single chromosomal fragments. Alternatively, the plurality of chromosomal fragments may be sequenced together to provide unphased nucleic acid sequence for the chromosomal fragments. | 06-14-2012 |
20120149589 | Epigentic Markers Associated with Substance Use Disorders - DNA methylations markers are associated with brain and behavioral mechanisms that underlie substance abuse disorders. These methylation markers present novel measures for predicting and/or identifying effective treatment options, risk of cancer development, risk of developing substance abuse disorders, and substance-abuse related behaviors such as binge drinking. These markers may further be useful in developing novel pharmaceuticals and treatment methodologies and provide mechanisms for following the course of an individual's treatment, risks, or behaviors over time. | 06-14-2012 |
20120157326 | STEM ACCELERATED ISOTHERMAL NUCLEIC ACID AMPLIFICATION TECHNOLOGY - The invention is in the field of nucleic acid amplification, hi particular, methods are described which utilise stem primers that improve the rapid and specific amplification of a test sample. | 06-21-2012 |
20120157327 | Labelling Strategies for the Sensitive Detection of Analytes - The present invention relates to methods and reagents for detecting analytes, e.g. nucleic acids. The new methods and reagents allow a simple and sensitive detection even in complex biological samples. | 06-21-2012 |
20120165205 | METHOD FOR SEQUENCING A POLYNUCLEOTIDE TEMPLATE - A method of determining the sequence of a target nucleic acid is provided. The method can include the steps of (a) performing a defined number of incremental extension cycles to produce a population of nucleic acid fragments having different portions of the target nucleic acid wherein the individual nucleic acid fragments in the population have a defined length that is correlated with the number of incremental extension cycles; (b) determining the sequence of the first end of individual nucleic acid fragments in the population, thereby providing first end sequences; (c) determining the sequence of the second end of individual nucleic acid fragments in the population, thereby providing second end sequences; and (d) determining the sequence of the target nucleic acid based on the first end sequences, the second end sequences and the defined length. | 06-28-2012 |
20120172240 | GENETIC POLYMORPHISMS ASSOCIATED WITH ADVANCED LUNG DISEASES - The present invention relates to methods and compositions that may be used to predict the risk of an individual, for example a smoker, for developing chronic obstructive pulmonary disease (“COPD”), emphysema or idiopathic pulmonary fibrosis (“IPF”). | 07-05-2012 |
20120172241 | MODELS FOR ANALYZING DATA FROM SEQUENCING-BY-SYNTHESIS OPERATIONS - Mathematical models for the analysis of signal data generated by sequencing of a polynucleotide strand using a pH-based method of detecting nucleotide incorporation(s). In an embodiment, the measured output signal from the reaction confinement region of a reactor array is mathematically modeled. The output signal may be modeled as a linear combination of one or more signal components, including a background signal component. This model is solved to determine the nucleotide incorporation signal. In another embodiment, the incorporation signal from the reaction confinement region of a reactor array is mathematically modeled. | 07-05-2012 |
20120178636 | METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION - Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products. | 07-12-2012 |
20120184448 | Biomarker-Based Methods For Identifying And Formulating Compositions That Improve Skin Quality And Reduce The Visible Signs Of Aging In Skin - In various embodiments, provided are methods for selecting and formulating compositions for treating and maintaining the quality of skin, wherein a composition is selected for use in a personal care product based on its demonstrated biological effect to improve skin quality as evidenced by one or more biomarker changes that correlate with improvement as evidenced by one or more objective measurements of skin health. | 07-19-2012 |
20120184449 | FETAL GENETIC VARIATION DETECTION - Provided herein are fetal diagnostic methods, kits and computational products useful for non-invasively detecting genetic variations for which maternal nucleic acid sequences are utilized as a reference. | 07-19-2012 |
20120184450 | HIGH THROUGHPUT SCREEN FOR MEASURING MEMBRANE EFFECTS - The invention relates to assays for measuring the effect of a inactive test substance on a lipid bilayer, kits for measuring the effects of test substances on lipid bilayers and an apparatus for performing a high through-put assay that measures the effect of test substances on a lipid bilayer. | 07-19-2012 |
20120190561 | MEANS AND METHODS FOR DIAGNOSING ENDOMETRIOSIS - The present invention relates to means and methods for diagnosing or predicting endometriosis in a female subject. Particularly, the present invention relates to methods for determining the susceptibility to, predisposition for, presence of and/or risk of developing or suffering from endometriosis in a female subject. The present invention also relates to a kit useful for determining the risk of developing or suffering from endometriosis in a subject, a binding molecule specifically binding to the DBP GC*2 or DBP GC*1 allele product, and a binding molecule binding to the gene encoding the DBP GC*2 or GC*1 allele. | 07-26-2012 |
20120196759 | TEMPERATURE REGULATION OF MEASUREMENT ARRAYS - A system for regulating a temperature of a measurement array is disclosed. The system includes a measurement array including a plurality of sensors, wherein the plurality of sensors are integrated onto an integrated circuit die. The system includes a thermal sensor integrated onto the integrated circuit die, wherein the thermal sensor senses a temperature associated with the plurality of sensors. The system further includes a heat pump coupled to the integrated circuit die, wherein the heat pump is controlled by a feedback control circuit including the thermal sensor. | 08-02-2012 |
20120220470 | DIAGNOSTIC METHOD FOR DETECTING CANCER BY MEASURING AMOUNT OF A CYTOKINE LIKE IL-6 - The present invention relates to a diagnostic method for predicting the possible recurrence of tumours in cancer patients. The method comprises culturing blood cells from a patient suffering from cancer in the presence of a cytokine stimulating factor, where after the amount of induced cytokine thereby produced is determined giving an indication of the risk of recurrence of the cancer. | 08-30-2012 |
20120220471 | NANOANTENNA AND USES THEREOF FOR BIOSENSING - A metal nanoantenna for use in a biosensing device is disclosed. The metal nanoantenna is arranged to exhibit at least two particle plasmon resonances or surface plasmon resonances (SPRs). The nanoantenna is for use in a sensor and allows detection at low concentration of biological components. In one aspect, the nanoantenna can have an asymmetric structural configuration and spectrally separated resonances. In one aspect, there is a location in its structure providing local electromagnetic field enhancement at all of the SPRs. The metal nanoantenna can be used for background free measuring of a quantity of a biological component. | 08-30-2012 |
20120231960 | SYSTEMS AND METHODS FOR HIGH-THROUGHPUT DETECTION OF AN ANALYTE IN A SAMPLE - Provided are high-throughput detection systems. The systems include a magnetic sensor device, a magnetic field source and a reservoir plate that includes a plurality of fluid reservoirs. The magnetic sensor device includes a support with two or more elongated regions each having a magnetic sensor array disposed at a distal end. Also provided are methods in which the subject high-throughput detection systems find use. | 09-13-2012 |
20120231961 | METHODS AND SYSTEMS FOR SPORES DETECTION - Provided herein are methods and systems for the detection of spores in a sample which comprise permeabilizing a protein-based spore coat with a protein degrading agent comprising a non-ionic detergent and in particular with a specific mixture of various protein degrading agents comprising a non-ionic detergent to allow contact of spore nucleic acids with fluorescent reagents suitable for detection. | 09-13-2012 |
20120238458 | Novel tumor marker determination - A method of determining gynaecologic tumor disease in a subject by providing a sample of peripheral blood of the subject, measuring the PPIC expression of cells in the sample, and comparing this to a reference value, the PPIC overexpression being indicative of a gynecologic tumor disease and/or disease progression, including metastatic potential in a gynecologic cancer patient. | 09-20-2012 |
20120238459 | METHOD FOR PREDICTING THERAPEUTIC EFFECT ON CHRONIC HEPATITIS C - A method for predicting therapeutic effect of combination therapy with peginterferon and ribavirin in chronic hepatitis C therapy, comprising:
| 09-20-2012 |
20120245042 | DEBUBBLER FOR MICROFLUIDIC SYSTEMS - Provided are robust, passive, membrane-based debubblers that are readily incorporated into microfluidic devices for rapid degassing. Also provided are methods of degassing fluid disposed within fluidic systems. | 09-27-2012 |
20120245043 | SYSTEMS AND METHODS FOR DETECTING AND IDENTIFYING CONTAMINANTS IN A GASEOUS ENVIRONMENT - A method for identifying a contaminant in an environment includes providing a sensor array, the sensor array including a plurality of sensing platforms, each of the sensing platforms including a corrodible metal. A reaction is detected on the corrodible metal on one or more of the sensing platforms to identify a reaction pattern, and the reaction pattern is compared to known reaction characteristics of the corrodible metals. Based on this comparison, the contaminant, such as a corrosive gas, can be identified. The sensing platform may include a quartz crystal microbalance or a nanostructure. In some features, at least one of the corrodible metals includes gold, and a detected reaction of the gold corrodible metal indicates the presence of adverse temperature or humidity conditions in the environment. | 09-27-2012 |
20120245044 | METHODS OF DETERMINING CHEMOTHERAPY RESPONSE IN CANCER - This disclosure provides a biomarker profile, which is linked to cancer cell chemo-resistance. The disclosure further provides methods of diagnosis and theranosis, and screening of new therapeutic agents using these biomarkers in the profile, and kits for employing these methods and compositions. | 09-27-2012 |
20120252682 | METHODS AND SYSTEMS FOR SEQUENCING NUCLEIC ACIDS - The present invention provides methods and systems for sequencing long nucleic acid fragments. | 10-04-2012 |
20120258873 | REDUCTION OF 2,3-DIHYDROXY-2-METHYL BUTYRATE (DHMB) IN BUTANOL PRODUCTION - The invention relates generally to the field of industrial microbiology and butanol production. More specifically, the invention relates methods of reducing 2,3-dihydroxy-2-methyl butyrate (DHMB) in butanol production. DHMB can be reduced by inhibiting the reduction of acetolactate to DHMB, for example, by knocking out enzymes that catalyze the reduction or by removing DHMB during or after fermentation. Yeast strains, compositions, and methods for reducing DHMB and increasing butanol yield are provided. | 10-11-2012 |
20120264624 | METHOD AND DEVICE FOR DETECTING ANALYTES IN A SAMPLE LIQUID - A method for detecting analytes in a sample liquid is provided in which the sample liquid is subjected to a mixing treatment on a surface of a support having in particular immobilized reactants, wherein an in particular trough-like vessel with an essentially flat bottom as a support surface for the immobilized reactants is used as the vessel for the sample liquid. The sample liquid is moved relative to the immobilized reactants by an oscillating movement of the vessel during the mixing treatment in one direction, typically along a line or a curve. In addition, a device is provided for carrying out the method according to the present disclosure. | 10-18-2012 |
20120270742 | Obesity Dependent Adipokine Biomarkers for Prostate Cancer - There is provided herein adipokine biomarkers for prostate cancer and methods and uses thereof. | 10-25-2012 |
20120283111 | Bacterial mRNA screen strategy for novel pesticide-encoding nucleic acid molecule discovery - Methods are provided for isolating and identifying novel pesticide-encoding nucleic acid molecules from bacterial strains identified as having a pesticidal polypeptide. The methods involve selecting a bacterial strain having or suspected of having at least one pesticide-encoding plasmid, curing the at least one pesticide-encoding plasmid from the bacterial strain and performing a subtractive hybridization to obtain plasmid mRNA encoding the pesticidal polypeptide. The plasmid mRNA can be used to make a cDNA library from which the pesticidal polypeptide can be isolated and subsequently identified. | 11-08-2012 |
20120289416 | METHODS AND KITS USED IN ASSESSING CANCER RISK - Methods of assessing the risk of recurrence of endometrial cancer on the basis of the presence or absence of mutations in FGFR2 are disclosed. | 11-15-2012 |
20120309635 | Multi-Sample Particle Analyzer and Method for High Throughput Screening - Embodiments of the present invention provide a system and method for analyzing a plurality of samples comprising obtaining with an autosampler a plurality of samples from a first plate having a plurality of sample wells wherein the autosampler has a plurality of probes for sampling a set of samples and wherein each probe of the plurality of probes is in communication with a separate flow cytometer via a separate conduit. The plurality of samples comprising particles is moved into a fluid flow stream for each separate conduit. Adjacent ones of the plurality of samples are separated from each other in the fluid flow stream by a separation gas, thereby forming a gas-separated fluid flow stream. The gas-separated fluid flow stream is independently guided to and through each separate flow cytometer. | 12-06-2012 |
20120322670 | IDENTIFYING GENETIC VARIATION IN AFFECTED TISSUES - Methods for the determination of tissue-specific genetic variation are provided. For example, in certain aspects methods for using iPS cell-derived specific cell types for differential molecular analysis of tissue-specific genetic variation are described. | 12-20-2012 |
20120329662 | HYPOXIA TUMOUR MARKERS - The present invention relates to a method for assessing a hypoxia phenotype of a tumour of a subject in which the gene expression of between 3 and 50 hypoxia-related genes of a sample obtained from said tumour of the subject is determined, thereby obtaining a sample expression profile of said hypoxia-related genes. The sample gene expression profile is then compared with a reference expression profile of said hypoxia-related genes. The hypoxia-related genes comprise at least SLC2A1, VEGFA and PGAM1. Probes, arrays and kits for use in the method are also disclosed. | 12-27-2012 |
20130005589 | HIGHLY SENSITIVE METHOD FOR DETECTING MUTATED GENE - Various highly sensitive detection methods, particularly improved PNA-LNA-PCR clamp methods, are provided as methods for detecting the presence or absence of a mutated gene contained in a gene pool rapidly, in a simple manner, with high accuracy, and with high sensitivity. As a step before the main step for detection, a pre-amplification step comprising allowing (1) a clamp primer consisting of PNA which hybridizes with all or part of a target site having a sequence of a wild-type gene or a sequence complementary to the wild-type gene, (2) a primer capable of amplifying a region comprising a target site having a sequence of the mutated gene, and (3) the gene pool to coexist in a reaction solution for gene amplification, and selectively amplifying the region comprising a target site of the mutated gene by a gene amplification method. | 01-03-2013 |
20130023423 | TRANSPOSON NUCLEIC ACIDS COMPRISING A CALIBRATION SEQUENCE FOR DNA SEQUENCING - Transposon nucleic acids comprising a transposon end sequence and a calibration sequence for DNA sequencing in the transposon end sequence. In one embodiment, the transposon end sequence is a Mu transposon end. A method for the generation of DNA fragmentation library based on a transposition reaction in the presence of a transposon end with the calibration sequence providing facilitated downstream handling of the produced DNA fragments, e.g., in the generation of sequencing templates. | 01-24-2013 |
20130023424 | DETECTING PAX2 FOR THE DIAGNOSIS OF BREAST CANCER - A method for monitoring breast conditions in a subject is disclosed. The method comprises determining a Paired Box 2 gene-to-beta defensin-1 gene (PAX2-to-DEFB1) expression ratio (the “Donald Predictive Factor” or “DPF”) in cells obtained from the breast of the subject, wherein the PAX2-to-DEFB1 expression ratio is correlated with breast conditions. Also disclosed is a kit for monitoring breast conditions and determining drug resistance. | 01-24-2013 |
20130023425 | DETECTING PAX2 FOR THE DIAGNOSIS OF BREAST CANCER - A method for monitoring breast conditions in a subject is disclosed. The method comprises determining a Paired Box 2 gene-to-beta defensin-1 gene (PAX2-to-DEFB1) expression ratio (the “Donald Predictive Factor” or “DPF”) in cells obtained from the breast of the subject, wherein the PAX2-to-DEFB1 expression ratio is correlated with breast conditions. Also disclosed is a kit for monitoring breast conditions and determining drug resistance. | 01-24-2013 |
20130029856 | Transfer Or Interrogation Of Materials By Carrier And Receiving Devices Moving Independently And Simultaneously On Multiple Axes - Material transfer/interrogation devices (e.g. liquid handling workstations) have been designed in the past for transferring material from a source to a destination location or for interrogating a material at a location, where the locations remain fixed. The invention provides methods and apparatuses for transferring or interrogating materials by one or more carrier devices to one or more receiving devices, where the carrier and receiving devices move independently and simultaneously on multiple axes. In some embodiments, one or more of the carrier and receiving devices can move along an X, Z, Y, and Theta axis, which allows the source and destination locations to rotate and translate relative to each other. Due to this rotation and translation, containers can be positioned to minimize the distance traveled between a pick location from the source and a place location on the destination, greatly increasing the speed at which material transfer can occur. | 01-31-2013 |
20130035243 | KLF6 ALTERNATIVE SPLICE FORMS AND A GERMLINE KLF6 DNA POLYMORPHISM ASSOCIATED WITH INCREASED CANCER RISK - Disclosed are methods of identifying and diagnosing certain types of cancers and pre-stages thereof in a patient by identifying alternatively spliced isoforms of wild type KLF6 (KLF6wt), in particular any one of the isoforms selected from the group consisting of: KLF6 splice variant-1 (KLF6 | 02-07-2013 |
20130040829 | IN VITRO MODEL OF LATENT MYCOBACTERIAL INFECTION - A method of inducing latency in | 02-14-2013 |
20130040830 | Fluid Sample Testing System - A sample testing vessel may include a flexible plastic tube and a self-sealing injection channel. The flexible plastic tube may have a seal defining a first compartment and a second compartment, wherein the seal comprises a pressure gate providing a fluid-tight seal between first and second compartments and opening upon application of a threshold pressure. The injection channel may be normally substantially free of fluid and capable of fluid communication with the tubule. | 02-14-2013 |
20130045875 | LIBRARY CHARACTERIZATION BY DIGITAL ASSAY - Methods of characterizing a nucleic acid library by digital assay. | 02-21-2013 |
20130045876 | INTEGRATED MICROFLUIDIC AND SOLID STATE PYROSEQUENCING SYSTEMS - The invention provides for sequencing a nucleic acid molecule based on the detection of base incorporation by the release of pyrophosphate (PPi) using a new enzyme system comprising adenosine diphosphate (ADP)-glucose pyrophosphorylase (AGPase) and its substrate ADP-glucose. | 02-21-2013 |
20130045877 | METHODS TO FORM SUBSTRATES FOR OPTICAL SENSING BY SURFACE ENHANCED RAMAN SPECTROSCOPY (SERS) AND SUBSTRATES FORMED BY THE METHODS - A method of manufacturing a substrate is provided. The method comprises, in some aspects, a) providing a support; b) forming a template by attaching a plurality of polymeric nanoparticles some or all having a core-shell structure to the support, wherein the core comprises a first polymer and the shell comprises a second polymer; and c) forming the metal nanoarray substrate by attaching a plurality of metallic nanoparticles to at least some of the polymeric nanoparticles of the template. A biosensor comprising a substrate manufactured by the method, and a method for the detection of an analyte in a sample by surface enhanced Raman spectroscopy (SERS) is also provided. | 02-21-2013 |
20130072388 | PHOTOCLEAVABLE LABELED NUCLEOTIDES AND NUCLEOSIDES AND METHODS FOR THEIR USE IN IN DNA SEQUENCING - Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3′-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format. The design of such rapidly cleavable fluorescent groups on nucleotides and nucleosides can enhance the speed and accuracy of sequencing of large oligomers of DNA in parallel, to allow rapid whole genome sequencing, and the identification of polymorphisms and other valuable genetic information, as well as allowing further manipulation and analysis of nucleic acid molecules in their native state following cleavage of the fluorescent group. | 03-21-2013 |
20130102479 | METHODS OF ENHANCING PLURIPOTENTCY - We provide for the use of Tbx3 (GenBank Accession Number: NM_005996.3 (SEQ ID NO. 1), NP_005987.3 (SEQ ID NO. 2), NM_016569.3 (SEQ ID NO. 3), NP_057653.3 (SEQ ID NO. 4)) in a method of enhancing or inducing pluripotency in a cell such as a somatic cell. We describe a method of reprogramming a cell, the method comprising modulating the expression and/or activity of Tbx3 in the cell. The cell may become a pluripotent cell such as a stem cell. We further describe a method of causing a cell such as a somatic cell to display one or more characteristics of a pluripotent cell, the method comprising modulating the expression and/or activity of Tbx3 in the cell. The method may further comprise modulating the expression and/or activity of one or more, a combination of or all of Oct4, Sox2 and Klf4 in the cell. | 04-25-2013 |
20130109579 | ANALYSIS OF Y-CHROMOSOME STR MARKERS | 05-02-2013 |
20130123119 | DETECTION OF FOOD THREAT AGENTS AND FOOD-BORNE PATHOGENS - Disclosed are novel primers for use in the molecular detection of food-threat agents and food-borne pathogens. The primers may be used in combination for the rapid, high-throughput screening PCR-based techniques to simultaneously detect multiple food safety biothreat agents. The multiplex-detection methods have improved sensitivity and specificity for the detection of multiple high-impact food-borne pathogens simultaneously. Real-time PCR assaying techniques using such primers include microarrays and multiplex single-tube arrays, the latter optionally simultaneously with TaqMan probes. | 05-16-2013 |
20130130923 | PROCESSES AND KITS FOR IDENTIFYING ANEUPLOIDY - Provided are methods for identifying the presence or absence of a chromosome abnormality by which a cell-free sample nucleic acid from a subject is analyzed. In certain embodiments, provided are methods for identifying the presence or absence of a fetal chromosome abnormality in a nucleic acid from cell-free maternal blood. | 05-23-2013 |
20130137589 | ANALYSIS OF Y-CHROMOSOME STR MARKERS - The methods and compositions provided herein relate to the discovery of 13 STR markers, found on the human Y chromosome, having surprisingly high mutation rates when compared with 173 other Y-STR markers known today. The set of RM-Y-STRs may overcome the current dilemma of Y-chromosome analysis in forensic applications due to their extraordinary mutation properties. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers. | 05-30-2013 |
20130143747 | METHODS OF DETECTING CANCER - Methods and compositions involving molecular markers for the detection and characterization of cancer in a patient are provided. | 06-06-2013 |
20130143748 | METHODS FOR DETECTION OF RISK OF OBESITY AND RISK OF ONSET OF DIABETES - Provided is a method of measuring the expression level of FSTL3 gene in a biological sample and correlating the measured expression level with the detection of a risk of developing diabetes. Also provided is a method of measuring the expression level of FSTL3 gene in an individual with a BMI value less than 25 not clinically determined as obesity and correlating the measured expression level with the detection of a risk of developing obesity. Further provided is a method of measuring the expression level of FSTL3 gene in an individual with a BMI value less than 25 and correlating the measured expression level with the detection of a risk of developing diabetes. Further provided is a method of measuring an inhibin βB gene expression level and correlating the ratio of expression level of FSTL3 gene to inhibin βB gene with the detection of a risk of developing obesity or diabetes. | 06-06-2013 |
20130157877 | BIOCHIPS FOR ANALYZING NUCLEIC ACID MOLECULE DYNAMICS - The invention relates to biochips | 06-20-2013 |
20130172200 | METHOD OF ASSESSING CANCEROUS CONDITIONS AND REAGENT FOR DETECTING GENE PRODUCT TO BE USED IN THE METHOD - A reagent for detecting gene product, the reagent comprising a probe or chemical modulation that specifically binds to an alternative splicing junction of a gene product of human PTCH1 gene, the expression of the gene product from gene products of the human PTCH1 gene being varied due to the unusual alternative splicing, for use for measuring the abundance of the gene product contained in a human sample. | 07-04-2013 |
20130172201 | ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS - A method for sequencing a polynucleotide strand by using sequencing-by-synthesis techniques. To address the problem of incomplete extension (IE) and/or carry forward (CF) errors that can occur in sequencing-by-synthesis reactions, an alternative flow ordering of dNTPs is used. In contrast to conventional flow orderings, the dNTPs are flowed in an ordering that is not a continuous repeat of an ordering of the four different dNTPs. This alternate flow ordering may reduce the loss of phasic synchrony in the population of template polynucleotide strands that result from IE and/or CF errors. | 07-04-2013 |
20130178375 | Methods of Detecting Neurological or Neuropsychiatric Diseases or Conditions - This invention provides methods of using phagocytic cells alone or in combination with non-phagocytic cells in the diagnosis, prognosis, or monitoring of neurological or neuropsychiatric diseases or conditions. The invention also provides methods of using phagocytic cells alone or in combination with non-phagocytic cells to identify markers of neurological or neuropsychiatric diseases or conditions. Provided are methods of using phagocytic cells alone or in combination with non-phagocytic cells in the diagnosis, prognosis, or monitoring of neurological or neuropsychiatric diseases or conditions. Also provided are methods of using phagocytic cells alone or in combination with nonphagocytic cells to identify markers of neurological or neuropsychiatric diseases or conditions. | 07-11-2013 |
20130178376 | Compositions and Methods for High-Throughput Nucleic Acid Analysis and Quality Control - Methods for improved quality control of nucleic acid containing biological samples utilized in high-throughput situations such as biorepositories engaged in the collection, processing, storage, distribution and analysis of such samples are disclosed. | 07-11-2013 |
20130203611 | AUTOMATIC SLIDE LOADING DEVICE FOR MICRO ARRAY SCANNER AND ITS METHODS OF USE - An automatic slide loading device for microarray scanner comprises slide holders ( | 08-08-2013 |
20130203612 | METHOD FOR ASSAYING SEPSIS IN HUMANS - The present invention relates to a reliable method of prediction of sepsis in humans after a trauma, wherein the level of pancreatic stone protein/regenerating protein (PSP/reg) is determined in serum, and a high level is indicative of the development of sepsis at early stages of the disease. Furthermore a method of determination of PSP/reg levels in serum is described. | 08-08-2013 |
20130210646 | HEMOMOSAIC: HIGH-THROUGHPUT TECHNIQUE FOR RARE CELL DETECTION IN LIQUID SAMPLES BY MASSIVELY MULTIPLEXED PCR IN A PHOTOLITHOGRAPHIC MATRIX - Described microfluidic technology focused on: 1) direct integration of microfluidic devices with solidified liquid tissue samples, 2) subordination of architectural and operational principles of microfluidic devices specific tissue structure and needs and/or 3) on-chip sample acquisition integrated with the detection measurement within the same device. In contrast to conventional methods of off-chip sample prep and subsequent insertion into a detection device, new applications are possible on solidified liquid or solid tissue samples, such as in situ PCR. | 08-15-2013 |
20130210647 | Methods of Detecting Cardiovascular Diseases or Conditions - Disclosed are methods of using phagocytic cells alone, or in combination with non-phagocytic cells, in the diagnosis, prognosis, or monitoring of cardiovascular diseases or conditions. Further disclosed are methods of using phagocytic cells alone or in combination with non-phagocytic cells to identify markers of cardiovascular diseases or conditions. | 08-15-2013 |
20130225424 | METHODS FOR DETERMINING RESPONSIVENESS TO A DRUG BASED UPON DETERMINATION OF RAS MUTATION AND/OR RAS AMPLIFICATION - The present disclosure provides methods for predicting the sensitivity (e.g., responsiveness) of a cell and/or biological sample obtained from a subject (e.g., a human) to a drug (e.g., a DHFR inhibitor). Such methods may comprise determining the presence or absence of one or more Ras mutations and/or determining the presence or absence of an amplification of the Ras gene in the cell and/or biological sample. The methods may be used to predict the responsiveness of a subject to treatment with a drug. | 08-29-2013 |
20130244889 | CONSTRUCT FOR PROMOTING ABSORPTION OF MOLECULES BY A CELL AND METHODS OF USING THE CONSTRUCT - The present invention is directed to a construct for promoting absorption of molecules by a cell and the application thereof in drug and gene delivery. The present invention further describes topographical modulation of endocytosis for drug and gene delivery. | 09-19-2013 |
20130261006 | Method of Generating a Hyperpigmentation Condition Gene Expression Signature - A method of generating a hyperpigmentation condition gene expression signature for use in identifying connections between perturbagens and genes associated with a skin pigmentation condition. The method includes providing a gene expression profile for a reference sample of human skin cells not affected with a pigmentation condition; generating a gene expression profile for a sample of human skin cells from a subject exhibiting the hyperpigmentation condition; comparing the expression profiles to determine a gene expression signature that includes a set of differentially expressed genes; assigning an identifier to each gene constituting the gene expression signature and ordering the identifiers according to the direction of differential expression to create one or more gene expression signature lists; and storing the one or more gene expression signature lists on at least one computer readable medium. | 10-03-2013 |
20130316921 | METHODS FOR DIAGNOSIS OF KAWASAKI DISEASE - Methods for diagnosis of Kawasaki disease (KD) are disclosed. In particular, the invention relates to the use of biomarkers for aiding diagnosis, prognosis, and treatment of KD, and more specifically to biomarkers that can be used to distinguish KD from other inflammatory diseases, including infectious illness and acute febrile illness. | 11-28-2013 |
20130324423 | NEW ANIMAL MODEL FOR DIABETIC COMPLICATIONS - The invention relates to the surprising find that low density lipoprotein receptor-deficient mice (LDLr−/−) mice when fed with high energy diets produce controllable and consistent diabetic complications, especially renal damage, similar to the human pathophysiology and biological response. The invention thus comprises a method for discovering a preventive or therapeutic regimen for the prevention or treatment of diabetic micro- or macrovascular complications, comprising the steps of: a. feeding LDLr−/− mice, which have not been treated with streptozotocin, with a high energy diet; b. before, during and/or after this diet treating the mice with the preventive or therapeutic regimen; c. checking whether any change in the micro- or macrovascular system of the animal occurs. Specifically in such a method renal damage is assessed. Also use of said mice fed with a high energy diet for studying the diabetic micro- and macrovascular complications is part of the invention. | 12-05-2013 |
20130324424 | SCANNING DROP SENSOR - Electrochemical experiments are performed on a collection of samples by suspending a drop of electrolyte solution between an electrochemical experiment probe and one of the samples that serves as a test sample. During the electrochemical experiment, the electrolyte solution is added to the drop and an output solution is removed from the drop. The probe and collection of samples can be moved relative to one another so the probe can be scanned across the samples. | 12-05-2013 |
20140005057 | PROTEIN VARIANT GENERATION BY REGION SHUFFLING | 01-02-2014 |
20140005058 | METHODS AND MATERIALS FOR THE DIAGNOSIS OF PROSTATE CANCERS | 01-02-2014 |
20140066319 | ALTERNATIVELY SPLICED mRNA ISOFORMS AS PROGNOSTIC INDICATORS FOR METASTATIC CANCER - The present invention provides a method for identifying a tumor as likely to metastasize, or likely to have metastasized, comprising obtaining a sample of the tumor and quantitating alternatively spliced mRNA isoforms of a cell motility gene, a cell adhesion gene and/or an actin cytoskeletal remodeling gene in the sample, or any specified genes or the level of RNA binding proteins compared to a predetermined non-metastasizing control. | 03-06-2014 |
20140087956 | NOVEL COMBINATION OF FLUORESCENT DYES FOR THE DETECTION OF NUCLEIC ACIDS - The present invention relates to combinations of fluorescent dyes used in molecular biology, particularly in multiplex PCR. In particular, the present invention relates to a combination of dyes for amplification reactions, wherein at least four different dyes are used, wherein the first dye is 5-FAM or 6-FAM or a blend thereof, the second dye is selected from the group consisting of DY-530, HEX, CAL Fluor Orange 560 and ATTO 532, the third dye is selected from the group consisting of ATTO 550, DY-555 and DY-556, the fourth dye is selected from the group consisting of ROX, DY-510XL and ATTO 565, and optionally a fifth dye is selected from the group consisting of DY 632 and DY-520XL. | 03-27-2014 |
20140141983 | METHODS OF DISEASE ACTIVITY PROFILING FOR PERSONALIZED THERAPY MANAGEMENT - The present invention provides methods for personalized therapeutic management of a disease in order to optimize therapy and/or monitor therapeutic efficacy. In particular, the present invention comprises measuring an array of one or a plurality of biomarkers at a plurality of time points over the course of therapy with a therapeutic agent to determine a mucosal healing index for selecting therapy, optimizing therapy, reducing toxicity, and/or monitoring the efficacy of therapeutic treatment. In certain instances, the therapeutic agent is a TNFα inhibitor for the treatment of a TNFα-mediated disease or disorder. | 05-22-2014 |
20140148348 | DECTECTION OF GASTROINTESTINAL DISORDERS - Provided herein are methods and systems for characterizing a phenotype by detecting microRNAs, vesicles, or biomarkers that are indicative of disease or disease progress. The disease can be a gastrointestinal disorder, such as colorectal cancer. The microRNAs, vesicles, or biomarkers can be detected in a bodily fluid. | 05-29-2014 |
20140148349 | Metabolite Biomarkers for the Detection of Esophageal Cancer Using NMR - Methods for the detection and screening of esophageal adenocarcinoma (EAC) patients and for the monitoring of EAC treatment using a panel or panels of small molecule metabolite biomarkers are disclosed. In other aspects, methods for detection and screening for the progression of high-risk conditions (BE and HGD) to EAC and to monitoring treatment using a panel or panels of small molecule metabolite biomarkers are disclosed. The biomarkers are sensitive and specific for the detection of EAC, and can also be used to classify Barrett's esophagus (BE) and high-grade dysplasia (HGD), which are widely regarded as precursors of EAC. | 05-29-2014 |
20140155279 | Detection of a panel of urine DNA markers for HCC screening and disease management - Provided herein is a method for detecting the presence or absence of a cancer in a biological sample of an individual, by determining the level of mutation and methylation of one or more genes from a group of genes comprising TP53, CTNNB1, hTERT, RASSF1A, GSTP1, p16, p15 and SFRP-1. Also provided herein is an assay to detect p53 mutations suitable for DNA isolated from biological body fluid in order to screen cancer patients. | 06-05-2014 |
20140155280 | METHOD OF DIAGNOSING NEOPLASMS - The present invention relates generally to a method for screening a subject for the onset, predisposition to the onset and/or progression of a colorectal neoplasm by screening for modulation in the level of expression of one or more nucleic acid markers. More particularly, the present invention provides a method for screening a subject for the onset, predisposition to the onset and/or progression of a colorectal neoplasm by screening for modulation in the level of expression of one or more gene markers in membranous microvesicles. The expression profiles of the present invention are useful in a range of applications including, but not limited to, those relating to the diagnosing and/or monitoring of colorectal neoplasms, such as colorectal adenoma and adenocarcinomas. | 06-05-2014 |
20140179539 | Novel Ligase Activity - Compositions and methods are provided for ligating polynucleotides having a length that is greater than 8 nucleotides on an RNA splint. The ligation reaction provides consistent results in high or low ATP concentrations. The reaction can occur rapidly and is generally at least 10 fold more efficient than T4DNA ligase under optimal conditions for T4DNA ligase and the reaction time is less than 6 hours for example, less than 1 hour. | 06-26-2014 |
20140243218 | COMPOSITION FOR DIAGNOSING OVARIAN CANCER METASTASIS USING CPG METHYLATION STATUS OF GENE PROMOTER AND USE THEREOF - The present invention relates to a composition, a kit and a method for diagnosing ovarian cancer metastasis or predicting the risk of metastasis by detecting methylation levels at CpG sites of one or more gene promoters selected from the group consisting of AGR2 (anterior gradient 2), CA9 (carbonic adj anhydrase 9), GABRP (gamma-aminobutyric acid receptor pi subunit), IFITM1 (interferon-induced transmembrane 1) and MUC13 (mucin 13). | 08-28-2014 |
20140243219 | METHODS AND SIGNATURES FOR OROPHARYNGEAL CANCER PROGNOSIS - Methods for determining the prognosis of a subject with oropharyngeal squamous cell carcinoma are described, as well as miRNAs and panels of prognostic miRNAs that are used in the methods. | 08-28-2014 |
20140256571 | Systems and Methods for Determining Copy Number Variation - A method of identifying a copy number variations reads includes mapping reads to a reference genome, computing coverage for a plurality of tiles, and normalizing the coverage for a tile based on a coverage mode across the plurality of tiles. The method further includes determining a score for the plurality of tiles being in a plurality of ploidy states, determining a maximum score path across the tiles and through the ploidy states, and providing a copy number determination based on the maximum score path. | 09-11-2014 |
20140274748 | DETECTING NEOPLASM - Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting premalignant and malignant neoplasms such as pancreatic and colorectal cancer. | 09-18-2014 |
20140287937 | METHODS FOR ASSESSING CANCER - Provided herein are methods for assessing cancer, comprising analysis of sequence data from a set of cancer-related genes in a tumor sample from a subject, followed by monitoring of a subset of the set in circulating tumor-associated DNA in a fluid sample from the subject. Also provided are kits and systems for practicing any of them methods of the invention. | 09-25-2014 |
20140287938 | RECOMBINANT VIRUS AND PREPARATIONS THEREOF - The present invention generally relates to methods and compositions used delivery of gene editing compositions including transcriptional effectors with parvovirus and preferred methods for making same. | 09-25-2014 |
20140296084 | Method for preparing nucleic acid library, its uses and kits - Provided are a method of constructing a nucleic acid library, a method of determining a nucleic acid sequence of a nucleic acid sample, and a kit thereof. The method of constructing the nucleic acid library includes the following steps: subjecting a nucleic acid sample to a DOP-PCR amplification, to obtain a first PCR amplification product; subjecting the first PCR amplification product to a second PCR amplification using a DOP-Amp primer, to obtain a second PCR amplification product; and subjecting the second PCR amplification product to an adaptor-ligation PCR, to obtain a third PCR amplification product, wherein the third PCR amplification product constitutes the nucleic acid library. | 10-02-2014 |
20140303010 | Methods and Compositions for Species-Specific Kinome Microarrays - A method of preparing a species-specific phosphorylation site peptide array for a target organism comprising: a) selecting a plurality of known non-target organism (NTO) phosphorylation site sequences and cognate known NTO phosphorylation polypeptide sequences from one or more NTO, each of the known NTO phosphorylation site sequences comprising at least 5 residues and less than 30 residues; b) identifying a matching target organism (TO) phosphorylation site sequence and cognate TO phosphorylation polypeptide sequence for one or more of the known NTO phosphorylation site sequences; c) determining the matching TO phosphorylation site sequences that correspond to orthologue polypeptides of the cognate known NTO phosphorylation polypeptide sequences; d) selecting the matching TO phosphorylation site sequences determined to correspond to orthologue polypeptides for inclusion on the array; wherein the matching TO phosphorylation site sequences that correspond to orthologue polypeptides are determined by calculating, for each matching phosphorylation site sequence identified in b), a similarity value between the TO phosphorylation polypeptide sequence corresponding to the TO phosphorylation site sequence and a TO polypeptide sequence matching the cognate known NTO polypeptide sequence. | 10-09-2014 |
20140329700 | METHODS OF ISOLATING RNA AND MAPPING OF POLYADENYLATION ISOFORMS - The invention relates to compositions and methods to isolate nucleic acids, and the identification of polyadenylation sites in a gene of interest. In one aspect, the invention provides an oligonucleotide comprising at least one nucleic acid and an affinity moiety, wherein said nucleic acid is 30-60 nucleotides in length and said nucleic acid comprises 1-25 uracil and 5-50 thymine nucleotides. | 11-06-2014 |
20140336062 | Multinode acoustic focusing for parallel flow cytometry analysis applications - An analytical device such as a flow cytometer is provided in which a fluid sample flowing through a channel is focused into multiple, parallel particle streams by an acoustic wave field extending across the channel. Each stream is then presented to an individual detector to allow for simultaneous interrogation of the multiple streams and thus, high-throughput analysis of the fluid sample. | 11-13-2014 |
20140349861 | Method for Distinguishing Biological Material Products - A method for encoding and identifying biological materials is disclosed. The method may include encoding and identifying plants from which controlled substances may be derived and other materials for which movement and distribution may need to be tracked. The biological material may be first encoded using DNA oligomers. A spray method or the use of an encoded substrate, both using these DNA oligomers for encoding the biological material, may be employed. The biological material, or a part of the biological material, may be first encoded by atomizing a solution containing DNA oligomers onto it and then dried by an appropriate method. Thereafter, the part of the encoded biological material, or the nitrocellulose substrate, may be dissolved with a buffer solution for extracting the DNA oligomers. Then, the dissolved solution may be used for generating a barcode by a suitable detection scheme. | 11-27-2014 |
20140357503 | METHOD AND ARRAY FOR IDENTIFYING HISTONE-CODE-RELATED ANALYTES - Disclosed embodiments concern an array for use in identifying or identifying and quantifying analytes in a sample using a macrocyclic sensor comprising a macrocyclic compound and a detectable moiety. The disclosed array may be used to discriminate among various analytes based on different features, such as post-translational modifications, isomeric post-translational modifications, and the peptide sequence around post-translational modifications. Also disclosed is a method for identifying analytes comprising a post-translational modification, as well as an enzymatic assay using the disclosed macrocyclic sensor. | 12-04-2014 |
20140364325 | Array of Sensors Functionalized with Systematically Varying Receptor Materials - A sensor array includes resonator sensors having respective receptor materials disposed thereon. The receptor materials have a physical property relevant to their ability to bind or adsorb one or more analytes in a sample. The physical property of the receptor materials on the sensors systematically increases or decreases in degree from one sensor to the next in the array. The device also comprises at least one detector for detecting sensor responses when masses of the analytes are adsorbed or bound to the receptor materials on the sensors. With this graded panel of sensors in the array, the analytes may adsorb or bind to the functionalized sensors with a pattern of responses specific to each analyte. | 12-11-2014 |
20150011404 | Microfluidic Bubble Logic Devices - Fluid-based no-moving part logic devices are constructed from complex sequences of micro- and nanofluidic channels, on-demand bubble/droplet modulators and generators for programming the devices, and micro- and nanofluidic droplet/bubble memory elements for storage and retrieval of biological or chemical elements. The input sequence of bubbles/droplets encodes information, with the output being another sequence of bubbles/droplets or on-chip chemical synthesis. For performing a set of reactions/tasks or process control, the modulators can be used to program the device by producing a precisely timed sequence of bubbles/droplets, resulting in a cascade of logic operations within the micro- or nanofluidic channel sequence, utilizing the generated droplets/bubbles as a control. The devices are based on the principle of minimum energy interfaces formed between the two fluid phases enclosed inside precise channel geometries. Various devices, including logic gates, non-volatile bistable memory, ring oscillators, bubble synchronizers, analysis chips, sample collectors, and printers have been designed. | 01-08-2015 |
20150057165 | MICRORNA AND USE THEREOF IN IDENTIFICATION OF B CELL MALIGNANCIES - Disclosed are nucleic acid sequences, including microRNA sequences and cDNA sequences, as well as vectors, DNA libraries, microarrays, and recombinant cells comprising the nucleic acid sequences described herein. Methods of determining the B cell stage from which a B cell malignancy is derived. Methods of identifying B cell malignancies are also provided. Methods of diagnosing B cell malignancies are provided. Such methods comprise, in certain embodiments, detecting one or more microRNAs or cDNAs as disclosed herein. | 02-26-2015 |
20150080236 | METHOD OF SUB-CLASSIFYING BREAST CANCER TUMORS - The method of sub-classifying breast cancer tumors profiles the expression of the Raf kinase inhibitor protein (RKIP) from a tissue sample from a cancerous primary breast tumor. The RKIP expression is profiled using its mRNA or by immunohistochemical protein quantifying methods in order to detect the level of RKIP in the breast cancer tissue sample. Based upon the RKIP expression profile, a sub-classification of cancer type may then be assigned. An RKIP expression of approximately 10.31 indicates basal carcinoma, an RKIP expression of approximately 10.04 indicates Claudin-low carcinoma, an RKIP expression of approximately 10.47 indicates Luminal-A carcinoma, an RKIP expression of approximately 10.44 indicates Luminal-B carcinoma, an RKIP expression of approximately 10.25 indicates HER2+ carcinoma, an RKIP expression of approximately 10.43 indicates ER+ carcinoma, and an RKIP expression of approximately 10.30 indicates ER− carcinoma. | 03-19-2015 |
20150087538 | Cancer Blood Test Using BC200 RNA Isolated from Peripheral Blood for Diagnosis and Treatment of Invasive Breast Cancer - Compositions and methods for diagnosing an increased risk for the development of aggressive, invasive breast cancer are provided. | 03-26-2015 |
20150099649 | METHODS AND MATERIALS FOR DETERMINING THE SOURCE OF WASTE - A method for identifying the source of animal waste is provided. The method includes taking DNA samples from a known group of animals, conducting DNA analysis on the DNA samples to prepare a genetic profile for each animal from the group, preparing a database of the genetic profiles, collecting a specimen of waste from an unknown source, conducting DNA analysis on the specimen, and comparing the DNA analysis from the specimen to the database to determine the source of the waste. | 04-09-2015 |
20150126382 | TECHNIQUE COMBINING PCR AND LOOP-MEDIATED ISOTHERMAL AMPLIFICATION FOR THE DETECTION OF NUCLEIC ACIDS - The present invention relates to a method and a kit of parts for detecting the presence or absence of one or more target nucleic acid sequences in a sample, the method comprising a sequence of steps for pre-amplifying the sample by means of a polymerase chain reaction, followed by a sequence of steps comprising an isothermal amplification of the pre-amplified sample, wherein the isothermal amplification comprises a pair of primers comprising a forward primer having a 3′ part that is substantially complementary to a first part of the target sequence, the presence or absence of which is to be detected, and a 5′ part that is substantially homolog to a second part of the target sequence, and a reverse primer comprising a 3′ part that is substantially homolog to a fourth part of the target sequence and a 5′ part that is substantially complementary to a third part of the target sequence. | 05-07-2015 |
20150309057 | Giant Liposome Array for High-throughput Lipid-Interaction Screening - The present invention relates to liposome arrays, devices comprising said liposome arrays, the uses of said liposome arrays as well as methods of producing said liposome arrays. | 10-29-2015 |
20150329836 | SPECIFIC INTERNALIZATION OF NANOPARTICLES INTO PROTEIN CAGES - The invention relates to a method to encapsulate nanoparticles into a protein cage by inserting the nanoparticles into the core through holes. Currently commercially available nanoparticles can be functionalized using the inventive method. The inventive hybrids have applications in biosensing and bioimaging. The use of an affinity between poly-histidine chains and nitrilotriacetic acid as chelating reagent to obtain the inventive cages and hybrid assemblies by the method according to the invention is shown in FIG. | 11-19-2015 |
20150369814 | USE OF RNA REMOVAL TO INITIATE PROTEIN AGGREGATION - The present invention provides the use of RNA removal to initiate protein aggregation of a plurality of proteins in a cell or cell lysate. This may be used to create an in vitro model of a disease, such as a neurodegenerative disease. The present invention also provides a method for determining the efficacy of a potential anti-protein aggregation agent comprising the following steps: i) using RNA removal to initiate the aggregation of a protein in a cell or cell lysate, ii) treating the cell or cell lysate with the potential anti-protein aggregation agent before, after or during RNA removal; and iii) comparing protein aggregation in equivalent samples with and without step ii) treatment in which a decrease in protein aggregation associated with step ii) treatment indicates that the potential anti-protein aggregation agent is effective in preventing and/or reversing protein aggregation. | 12-24-2015 |
20150376693 | Sieving and Profiling Nucleic Acid Samples - A method of selecting nucleic acid samples including particular desired alleles from a plurality of nucleic acid samples including the steps of performing a first reaction in a plurality of pools containing the samples to produce reaction products including a source tag identifying said each pool; pooling the pools to provide pooled pools; for each of the desired alleles to be identified, performing a second reaction using said reaction products to produce allele-specific second reaction products comprising a marker tag and a derived source tag; identifying said allele-specific second reaction products, and further identifying nucleic acid samples with the desired alleles. A source tag sharing number “d” may be determined for each of the alleles. Alleles may also be binned together. | 12-31-2015 |
20150376712 | METHODS FOR DIAGNOSIS, PROGNOSIS AND TREATMENT OF PRIMARY AND METASTATIC BASAL-LIKE BREAST CANCER AND OTHER CANCER TYPES - In one embodiment, a method of theranostic classification of a breast cancer tumor is provided, comprising obtaining a breast cancer tumor sample from a subject, detecting an expression level of FOXC1, comparing the expression level of FOXC1 to a predetermined cutoff level, and classifying the breast cancer tumor sample as belonging to a theranostic basal-like breast cancer tumor subtype or a theranostic hybrid basal-like breast cancer tumor subtype when the expression level of FOXC1 is higher than the predetermined cutoff level. In other embodiments, methods for predicting a prognosis of a basal-like breast cancer and methods of treating a basal-like breast cancer are provided, | 12-31-2015 |
20160018406 | Determination of Protein Aggregation from the Concentration Dependence of Delta G - The present invention relates to, among other things, methods and systems for recognizing and characterizing protein aggregation processes at the earliest possible time and use of such new methods and systems for (1) the identification and selection of protein formulations that minimize aggregation and extend long-term stability and (2) the identification of protein variants with the lowest tendency to aggregate. | 01-21-2016 |
20160019335 | METHOD, APPARATUS AND COMPUTER PROGRAM PRODUCT FOR METABOLOMICS ANALYSIS - A method is provided for analyzing metabolite data in a sample, comprising analyzing a sample to determine a first number of metabolites, and amount of each metabolite, included in the sample, and a second number of the first number of metabolites that are regulated. Biochemical pathways are determined, each having a third number of the first number of metabolites that are included in the sample and in the determined biochemical pathway. For each of the determined biochemical pathways having the third number of metabolites, a fourth number of the second number of metabolites that are included in the sample and in the determined biochemical pathway that are regulated metabolites is determined. Each of the third number of metabolites within one of the determined biochemical pathways is displayed and distinguished by the amount of each corresponding metabolite included in the sample. An associated apparatus and computer program product are also provided. | 01-21-2016 |
20160025761 | METHODS AND DEVICES FOR ANALYSIS OF DEFINED MULTICELLULAR COMBINATIONS - Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment, A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis. | 01-28-2016 |
20160033464 | METHODS FOR DETERMINING ACTIVE INGREDIENTS OF AN HERBAL MEDICINE, SOURCES OF AND CATALYTIC PATHWAYS FOR PRODUCTION THEREOF - Disclosed are methods for determining active ingredients of herbal medicines including identifying organic sources and catalytic (mineral) pathways to produce such active ingredients. | 02-04-2016 |
20160047720 | FILMS FOR BIOLOGIC ANALYTE COLLECTION AND ANALYSIS AND METHODS OF PRODUCTION AND USE THEREOF - Compositions, films, collection devices, apparatuses, kits and methods related to biologic analyte collection and analysis include thin films of modified polycaprolactone. Methods of production and use thereof are described herein. The films, compositions, collection devices, kits and methods can be used for collection of fingerprints for both image capture and nucleic acid extraction and analysis. | 02-18-2016 |
20160051960 | DEVICE AND METHOD FOR PRESSURE-DRIVEN PLUG TRANSPORT AND REACTION - The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid. | 02-25-2016 |
20160051986 | MICROFLUIDIC DEVICE AND METHOD FOR OPERATING THEREOF - The present invention provides a microfluidic device which comprises a drive module and a microfluidic platform. The drive module further comprises a rotary unit and a vibration unit for driving the microfluidic platform, and the microfluidic platform further comprises multiple microfluidic elements for performing tests. The present invention also provides a method for operating a microfluidic device. The method comprises steps using the rotary unit and steps using the vibration unit to distribute sample in a microfluidic structure. | 02-25-2016 |
20160061761 | SENSITIVITY AND SELECTIVITY OF CHEMORESISTOR TYPE GAS SENSORS - The sensitivity and/or selectivity of a chemoresistor type gas sensor is enhanced by measuring the response of the sensing material to a gas sample while the sensing material is subjected to illumination using specially-tailored pulses of ultraviolet radiation. For a given target gas to be detected there is an optimal duration of the UV pulses to achieve peak sensitivity of the sensing material. | 03-03-2016 |
20160068822 | RECOMBINANT VIRUS AND PREPARATIONS THEREOF - The present invention generally relates to methods and compositions used delivery of gene editing compositions including transcriptional effectors with parvovirus and preferred methods for making same. | 03-10-2016 |
20160096175 | SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS - A system for processing a plurality of biological samples contains a support and a temperature controller. The support is configured to hold a case that includes an inner chamber and a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples. The temperature controller is configured to maintain or control a temperature of at least one of the support, the case, or the one or more biological samples during an assay or reaction on the one or more biological samples. The support is also configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction. | 04-07-2016 |
20160097755 | METHOD FOR DETECTING, CAPTURING AND/OR RELEASING CHEMICAL ELEMENTS - The invention relates to a method suitable for detecting, capturing and/or selectively releasing chemical elements selected from poor metals, alkalines, alkaline earths, actinides and rare earths. Said method involves a molecular assembly formed by at least one amine, and at least one aldehyde and/or an imine and/or CO | 04-07-2016 |
20160110515 | METHOD AND SYSTEM FOR MICROBIOME-DERIVED DIAGNOSTICS AND THERAPEUTICS - A method for diagnosing and treating an immune microbial dysfunction in a subject, the method comprising: receiving an aggregate set of biological samples from a population of subjects; generating at least one of a microbiome composition dataset and a microbiome functional diversity dataset for the population of subjects; generating a characterization of the immune microbial dysfunction based upon features extracted from at least one of the microbiome composition dataset and the microbiome functional diversity dataset, wherein the characterization is diagnostic of at least one of Crohn's disease, inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), ulcerative colitis, and celiac disease; based upon the characterization, generating a therapy model configured to correct the immune microbial dysfunction; and at an output device associated with the subject, promoting a therapy to the subject based upon the characterization and the therapy model. | 04-21-2016 |
20160115472 | NON-DISRUPTIVE DNA ISOLATION FROM CORN SEEDS - This invention relates to systems and process for isolating DNA from biological materials such as seeds while retaining a viable seed for further use. The seed from which the DNA is isolated remains viable and is used or discarded based on the DNA analysis of the seed soak solution. The seed soak solution can have substantially all of the confounding maternal DNA from the seed eliminated from the seed soak solutions by employing intact seed pretreatments. This method is particularly useful for maize seed. | 04-28-2016 |
20160160262 | TEST KITS INCLUDING ACTIVATED MEDIATORS FOR DETECTING ANALYTES - Activated mediators for detecting predetermined analytes in a sample, as well as kits including testing surfaces incorporating an enzyme-activated mediator are disclosed. The enzyme-activated mediator may have a first color prior to the application of a sample to the portion of the testing surface including the enzyme-activated mediator. The enzyme-activated mediator is configured to undergo a detectable color change to a second color different from the first color in response to the sample applied to the testing surface including a predetermined analyte reactive with the enzyme-activated mediator. Methods for making the testing surfaces and using the testing surfaces to detect analytes are also disclosed. | 06-09-2016 |
20160161496 | Cells Having A Spectral Signature And Methods Of Preparation And Use Thereof - Methods, compositions and articles of manufacture for encoding a cell with semiconductor nanocrystals and/or other fluorophors are provided. The encoded cells can be subjected to functional assays in mixed populations, and an assay result can be determined and associated with individual cells by virtue of their code. The methods are particularly useful in multiplex settings where a plurality of encoded cells are to be assayed. | 06-09-2016 |
20160167046 | RIGID MASK FOR PROTECTING SELECTIVE PORTIONS OF A CHIP, AND USE OF THE RIGID MASK | 06-16-2016 |
20160169915 | BIOMARKERS OF AUTISM SPECTRUM DISORDER | 06-16-2016 |
20160187246 | SHEATH FLUID SYSTEMS AND METHODS FOR PARTICLE ANALYSIS IN BLOOD SAMPLES - Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer. | 06-30-2016 |
20160250636 | MICROREACTOR SYSTEM | 09-01-2016 |
20160377595 | PREDICTION OF ACUTE KIDNEY INJURY FROM A POST-SURGICAL METABOLIC BLOOD PANEL - Systems and methods are provided for predicting the likelihood of acute kidney injury. An input interface is configured to receive a plurality of features derived from the results of a post-surgical metabolic blood panel and either a pre-surgical metabolic blood panel or a perisurgical metabolic blood panel. A predictive model is configured to calculate a parameter representing a likelihood of acute kidney injury from the plurality of features. A user interface is configured to provide the calculated parameter to a user in a human comprehensible form. | 12-29-2016 |