Entries |
Document | Title | Date |
20080214404 | Functional molecule binding specifically to membrane protein, and manufacturing method therefor - A membrane protein is brought into contact with a pool of functional molecule candidates including a modified oligonucleotide sequence obtained by random polymerization of modified nucleotide n-mers (wherein n is an integer) having a modified nucleoside having a substituent introduced therein, then the membrane protein is treated with a protease, and the functional molecule that has bound to the membrane protein is isolated. The corresponding unmodified molecule is amplified from this function molecule, the sequence of corresponding unmodified molecules obtained by the amplification is decoded, and the decoding results are translated into the sequence of the isolated functional molecule. The functional molecule can be manufactured based on this translation. | 09-04-2008 |
20080248959 | GLYCOSYLATION OF PEPTIDES VIA O-LINKED GLYCOSYLATION SEQUENCES - The present invention provides sequon polypeptides with an amino acid sequence including one or more exogenous O-linked glycosylation sequence of the invention. In addition, the present invention provides methods of making polypeptide conjugates as well as methods of using such conjugates and their pharmaceutical compositions. The invention further provides libraries of sequon polypeptides, wherein each member of such library includes at least one exogenous O-linked glycosylation sequence of the invention. Also provided are methods of making and using such libraries. | 10-09-2008 |
20080254996 | Method of Analyzing Structure of Sugar Chain - It is an objective of the present invention to provide a method of analyzing the structure of a sugar chain by performing CID-MS | 10-16-2008 |
20090036317 | THERMOELECTRIC METHOD OF SEQUENCING NUCLEIC ACIDS - The present invention relates to a novel thermoelectric method for determining the sequence of nucleotides on a nucleic acid molecule through use of a thermopile and/or sequencing reagents flowing under the conditions of laminar flow. The methods disclosed herein involve the measurement of the heat generated by a deoxynucleotide incorporation event that can be accomplished without the need to control the temperature of any of a thermopile's junctions. | 02-05-2009 |
20090069189 | METHOD OF IDENTIFYING PROTEINS IN HUMAN SERUM INDICATIVE OF PATHOLOGIES OF HUMAN LUNG TISSUES - A method of identifying proteins present in human serum which are differentially expressed between normal individuals and patients known to have non-small cell lung cancers and asthma, as diagnosed by a physician. Human serum specimens from each population are digested with trypsin or any other suitable endoproteinase and analyzed using a liquid chromatography electrospray ionization mass spectrometer. Mass spectral data from each population is compared to determine proteins with expression intensities which are significantly differentially expressed between the normal, asthma, and lung cancer populations. Eleven proteins are found to have expression intensities which are significantly differentially expressed between the populations. Finally, the identities of the eleven proteins are obtained by comparing the mass spectral data with known databases having libraries of mass spectral data of known proteins. | 03-12-2009 |
20090075826 | Methods and apparatuses for sorting objects in forensic DNA analysis and medical diagnostics - The present invention relates to an apparatus and method of sorting objects and identifying the objects in a forensics sample, including using holographic optical trapping to sort objects from contaminants, and performing (single cell) PCR-based STR analysis on the objects to determine their identification. In addition, the chip used as a support for sorting the objects can also be used for performing single cell PCR-based STR analysis. In another embodiment, a microfluidics chip is used to stream the sample and sort the objects, before single cell PCR-based STR analysis is performed. The chip used for sorting utilizing HOT in the absence or presence of microfluidic streaming and sorting can also be the same as that used for the single cell PCR-based STR analysis. | 03-19-2009 |
20090186770 | Devices using addressable magnetic tunnel junction array to detect magnetic particles - A magnetic sensor for identifying small superparamagnetic particles bonded to a substrate contains a regular orthogonal array of MTJ cells formed beneath that substrate. A magnetic field imposed on the particle, perpendicular to the substrate, induces a magnetic field that has a component within the MTJ cells that is along the plane of the MTJ free layer. If that free layer has a low switching threshold, the induced field of the particle will create resistance changes in a group of MTJ cells that lie beneath it. These resistance changes will be distributed in a characteristic formation or signature that will indicate the presence of the particle. If the particle's field is insufficient to produce the free layer switching, then a biasing field can be added in the direction of the hard axis and the combination of this field and the induced field allows the presence of the particle to be determined. | 07-23-2009 |
20090203531 | Method for Archiving and Clonal Expansion - The present method provides methods, libraries, and kits related to the archiving and clonal expansion of sequences related to target polynucleotide sequences. The method allow for the attachment of polynucleotides with defined 3′ and or 5′ sequences to solid surfaces. The polynucleotides attached to the solid substrates can be stored or archived as libraries and can subsequently be retrieved for analysis, for example by clonal expansion. In some embodiments, nucleotides attached to solid surfaces can be used for sequencing of nucleotide sequences related to target RNA or target RNA. The methods are applicable to total RNA and/or total DNA analysis. | 08-13-2009 |
20090312188 | SYSTEM AND METHOD FOR NUCLEIC ACIDS SEQUENCING BY PHASED SYNTHESIS - A system and methods of sequencing a nucleic acid by detecting the identity of a fluorescent nucleotide analogue incorporated at the 3′ end of a growing nucleic acid strand are provided. One method includes the steps of (a) immobilizing a plurality of complexes comprising a template nucleic acid, a primer configured to hybridize to the template and a polymerase, at a plurality of optical sensing sites of a substrate, wherein the substrate is part of a waveguide-based optical scanning system; (b) extending the primer by a single nucleotide with the polymerase and one or more fluorescent nucleotide analogues using a polymerase extension reaction, wherein each type of fluorescent nucleotide analogue comprises a unique fluorescent tag optionally configured to inhibit further primer extension and/or a blocking agent at the 3′ end and wherein incorporation of the fluorescent nucleotide analogue reversibly terminates the polymerase extension reaction; (c) detecting the unique tag of the fluorescent nucleotide analogue by optically scanning the substrate using the optical scanning system to identify the fluorescent nucleotide analogue incorporated by the polymerase reaction; (d) recording the results of the optical scanning of the substrate; (e) reversing the termination of the polymerase extension reaction by providing a photo-cleaving pulse of light to one or more of the optical sensing sites of the substrate to cleave the fluorescent tag or the blocking agent; and (f) repeating steps (b) through (e). | 12-17-2009 |
20100022399 | METHODS FOR RAPIDLY IDENTIFYING SMALL ORGANIC MOLECULE LIGANDS FOR BINDING TO BIOLOGICAL TARGET MOLECULES - The present invention is directed to novel methods for rapidly and unambiguously identifying small organic molecule ligands for binding to biological target molecules. Small organic molecule ligands identified according to the methods of the present invention may find use, for example, as novel therapeutic drug lead compounds, enzyme inhibitors, labeling compounds, diagnostic reagents, affinity reagents for protein purification, and the like. Also presented are novel methods for identifying high affinity binding ligands for a biological target molecule of interest, wherein those methods comprise linking two or more small organic molecule ligands previously identified as being capable of binding to the biological target molecule of interest. Biological target molecules include, for example, polypeptides, nucleic acids, carbohydrates, nucleoproteins, glycoproteins, glycolipids and lipoproteins. | 01-28-2010 |
20100152051 | NOVEL METHOD FOR THE IDENTIFICATION OF CLONES CONFERRING A DESIRED BIOLOGICAL PROPERTY FROM AN EXPRESSION LIBRARY - The present invention relates to a novel method for the identification and/or characterization of clones conferring a desired biological property from an expression library. The method of the invention comprises the step of analyzing for the expression of at least one (poly)peptide, such as a tag expressed as a fusion protein, together with a recombinant insert of a clone of said expression library, wherein the clones of said expression library are arranged in arrayed form. Said (poly)peptide may be fused N-terminally or C-terminally to said insert. The method of the invention further comprises the steps of contacting a ligand specifically interacting with a (poly)peptide expressed by the insert of a clone conferring said desired biological property with a first replica of said library of clones in arrayed form and analyzing said library of clones for the occurrence of an interaction, and/or carrying out a hybridization or an oligonucleotide fingerprint with a nucleic acid probe specific for the insert of a clone conferring said desired biological property with a second replica of said library of clones arranged in arrayed form and analyzing said library of clones for the occurrence of a specific hybridization. Finally, the method of the invention requires the identification of clones wherein an expression of the at least one (poly)peptide in step (a) and/or an interaction in step (b) and/or a hybridization or an oligonucleotide fingerprint in step (c) can be detected. The present invention also relates to a kit useful for carrying out the method of the invention. | 06-17-2010 |
20100216648 | SYNTHESIS OF SEQUENCE-VERIFIED NUCLEIC ACIDS - The invention relates to methods and devices for preparing synthetic nucleic acids. | 08-26-2010 |
20100248971 | PROCESS FOR ANTIBODY PRODUCTION - A method of generating an antibody comprising the steps of: immunizing a tissue containing cells for immunization in vitro in a culture liquid containing an antigen and a stimulating substance, selecting the immunized cells, and obtaining antibodies from the above selected immunized cells. According to this method of generating an antibody, antibodies can be prepared in a short period of time in large quantities, thereby contributing to the increased use of immunological testing. | 09-30-2010 |
20100298153 | METHODS FOR ANALYSING PROTEIN SAMPLES BASED ON THE IDENTIFICATION OF C-TERMINAL PEPTIDES - The present invention relates to a method for identifying proteins in one or more samples based on the isolation and analysis of their C-terminal peptides. The isolated peptides are purified and analysed by Mass spectroscopy. Identification of the parent protein is based on the mass of the C-terminal peptide in combination with additional physicochemical parameters. The present invention further relates to an annotated database of C-terminal peptides of in silico cleaved proteins comprising the masses of C-terminal peptides and one or more physicochemical properties thereof. | 11-25-2010 |
20110003697 | PLANT MYB TRANSCRIPTION FACTOR HOMOLOGS - This invention relates to an isolated nucleic acid fragment encoding a Myb-related transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the Myb-related transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the Myb-related transcription factor in a transformed host cell. | 01-06-2011 |
20110105339 | PORTABLE PLASMA BASED DIAGNOSTIC APPARATUS AND DIAGNOSTIC METHOD - A portable plasma based diagnostic apparatus comprising a plasma source for producing energy projectiles atmospheric pressure, a mass analyzer, a sampling interface for receiving direct sample to be analyzed, the sampling interface being positioned between the plasma source and the mass analyzer, a database containing a library of biomarkers with their associated mass spectra, a processor operatively connected to the plasma source, the mass analyzer and the database. The processor is so configured so as to obtain from the mass analyzer a sample mass spectrum of parent and fragment ions resulting form the collision between the energetic projectiles and the sample, compare the sample mass spectrum with mass spectra in the reference library in order to identify at least one indicator and provide a report based on the at least one identified indicator. | 05-05-2011 |
20110118126 | METHODS OF CHEMOTYPE EVOLUTION - Herein is described a method to rapidly screen a large chemical space for a compound that binds to a target protein through an iterative fragment assembly approach that can be performed at low reagent cost and without requiring purification of the assembled product. The method employs a library of test ligands each of which comprise a ‘bait’ molecule, which is known from prior art or prior screening to have some intrinsic affinity for the target protein, and a test moiety. | 05-19-2011 |
20110136677 | METHODS OF BEAD MANIPULATION AND FORMING BEAD ARRAYS - According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same. | 06-09-2011 |
20110212843 | STRUCTURE BASED AND COMBINATORIALLY SELECTED OLIGONUCLEOSIDE PHOSPHOROTHIOATE AND PHOSPHORODITHIOATE APTAMER TARGETING AP-1 TRANSCRIPTION FACTORS - The present invention includes composition and methods for making and using a combinatorial library to identify modified thioaptamers that bind to, and affect the immune response of a host animal, transcription factors such as IL-6, NF-κB, AP-1 and the like. Composition and methods are also provided for the treatment of viral infections, as well as, vaccines and vaccine adjuvants are provided that modify host immune responses. | 09-01-2011 |
20110306504 | POLYNUCLEOTIDE MAPPING AND SEQUENCING - The present invention provides methods of obtaining structural information about a biopolymer sample. The methods include labeling portions of a biopolymer, such as DNA or RNA, linearizing the biopolymer in some cases, and determining the distance between the labels. The user can then compare different samples' between-label distances to qualitatively compare different samples and to assay a given sample for additions or deletions of nucleotides in the regions flanked by the labels. The methods also permit sequencing of biopolymers. | 12-15-2011 |
20110319275 | METHOD FOR IMMOBILIZING NUCLEIC ACIDS ON A SUPPORT - The present invention relates to a method for immobilizing nucleic acids on a support, comprising the provision of a nucleic acid with a stretch of nucleotides of only one basetype and the immobilization of said nucleic acid on a support by crosslinking by light, wherein said crosslinking by light is performed at a wavelength of about 300-500 nm, preferably at a wavelength of 365 nm. The present invention further relates to a method for the analysis of nucleic acids immobilized according to the invention, which comprises the hybridization of the immobilized nucleic acid with complementary and mismatch segments. Furthermore, the present invention relates to immobilized nucleic acids obtainable by the method of the invention, the use of accordingly immobilized nucleic acids for the production of nucleic acid arrays and a diagnostic kit, comprising an array of nucleic acids which are immobilized according to the present invention. | 12-29-2011 |
20120004114 | NUCLEOTIDE SEQUENCES ENCODING GSH1 POLYPEPTIDES AND METHODS OF USE - Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for improving agronomic traits, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a GSH1 polypeptide. | 01-05-2012 |
20120015825 | ANALYTICAL SYSTEMS AND METHODS WITH SOFTWARE MASK - Methods and systems for obtaining and processing optical signal data from analytical reactions, and in processing signal data from arrays of sequence-by-incorporation processes to identify nucleotide sequences of template nucleic acids and larger nucleic acid molecules, e.g., genomes or fragments thereof are described. Defining and applying a 2-dimensional software mask allows for obtaining signal data from arrays with higher signal to noise than where the mask is not applied. | 01-19-2012 |
20120015826 | METHODS FOR HIGH LEVEL MULTIPLEXED POLYMERASE CHAIN REACTIONS AND HOMOGENOUS MASS EXTENSION REACTIONS - Provided herein are optimized methods for performing multiplexed detection of a plurality of sequence variations. Also provided are methods for performing multiplexed amplification of target nucleic acid. | 01-19-2012 |
20120046179 | METHOD OF POOLING SAMPLES FOR PERFORMING A BIOLOGICAL ASSAY - The present invention relates among others to a method of pooling samples to be analyzed for a categorical variable, wherein the analysis involves a quantitative measurement of an analyte, said method of pooling samples comprising providing a pool of n samples wherein the amount of individual samples in the pool is such that the analytes in the samples are present in a molar ratio of x | 02-23-2012 |
20120065081 | NUCLEIC ACID CONSTRUCTS AND METHODS OF USE - The present invention provides oligonucleotide constructs, sets of such oligonucleotide constructs, and methods of using such oligonucleotide constructs to provide validated sequences or sets of validated sequences corresponding to desired ROIs. Such validated ROIs and constructs containing these have a wide variety of uses, including in synthetic biology, quantitative nucleic acid analysis, polymorphism and/or mutation screening, and the like. | 03-15-2012 |
20120108444 | COMPOSITIONS AND METHODS FOR DETECTING PREDISPOSITION TO A SUBSTANCE USE DISORDER - The present invention provides screening kits, compositions, and diagnostic methods for determining whether a subject has a predisposition to, or likelihood of having, a substance use disorder by determining a nucleic acid methylation profile from a biological sample from the subject, wherein a given profile indicates that the subject has a predisposition to a substance use disorder. | 05-03-2012 |
20120115737 | RESTRICTION ENDONUCLEASE ENHANCED POLYMORPHIC SEQUENCE DETECTION - Provided is an improved method for the detection of specific polymorphic alleles in a mixed DNA population. The method comprises enriching the relative percentage of a given polymorphic allele that is exponentially amplifiable by PCR. Also provided are methods for selectively enriching target nucleic acid, for example, fetal nucleic acid in a maternal sample. In the case of detecting fetal nucleic acid in a maternal sample, a restriction enzyme is introduced that can discriminate between the alleles of a polymorphic site. Preferably, the maternal allele is digested and nucleic acid comprising the paternal allele is relatively enriched. | 05-10-2012 |
20120149585 | COMPENSATOR FOR MULTIPLE SURFACE IMAGING - A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention. | 06-14-2012 |
20120172239 | METHOD FOR THE IDENTIFICATION BY MOLECULAR TECHNIQUES OF GENETIC VARIANTS THAT ENCODE NO D ANTIGEN (D-) AND ALTERED C ANTIGEN (C+W) - The invention relates to genotyping and blood cell antigen determination. In particular, the invention addresses discriminating the RHD*DIIIa-CE(4-7)-D or RHD*DIIIa-CE(4-7)-D)-like blood type variants, from RHD*DIIIa, RHD*DIVa-2 and other blood type variants. The invention provides methods for genotyping a subject, comprising:
| 07-05-2012 |
20120190560 | METHOD FOR AUTOMATED, LARGE-SCALE MEASUREMENT OF THE MOLECULAR FLUX RATES OF THE PROTEOME OR THE ORGANEOME USING MASS SPECTROMETRY - Disclosed here is a method for measuring the kinetics (i.e., the molecular flux rates—synthesis and breakdown or removal rates) of a plurality of proteins or organic metabolites inn living systems. The methods may be accomplished in a high-throughput, large-scale automated manner, by using existing mass spectrometric profiling techniques and art well known in the fields of static proteomics and static organeomics, without the need for additional biochemical preparative steps or analytic/instrumental devices. | 07-26-2012 |
20120220469 | TOXICITY TYPING USING LIVER STEM CELLS - This invention provides methods and systems for identifying and typing toxicity of chemical compositions, as well as for screening new compositions for toxicity. The invention involves detecting alterations in gene or protein expression and hence establishing molecular profiles in isolated mammalian LSCs contacted with various chemical compositions of known and unknown toxicities, and correlating the molecular profiles with toxicities of the chemical compositions. | 08-30-2012 |
20120258872 | PREVENTION, TREATMENT AND DIAGNOSIS OF DISEASES ASSOCIATED WITH BETA-AMYLOID FORMATION AND/OR AGGREGATION - The invention provides compositions and methods for prevention and treatment of diseases associated with β-amyloid formation and/or aggregation. Such methods encompass the induction of an immune response against N-terminal truncated and/or post-translationally modified Aβ peptides. These peptides are further used in compositions and methods for the diagnosis of diseases associated with β-amyloid formation and/or aggregation. | 10-11-2012 |
20120270741 | BIOMARKER DISCOVERY IN COMPLEX BIOLOGICAL FLUID USING BEAD OR PARTICLE BASED LIBRARIES AND DIAGNOSTIC KITS AND THERAPEUTICS - The present invention is useful in screening for biomarkers associated with any other disease or condition. Such diseases and conditions range from the neurological diseases, autoimmune diseases and cancers identified above as well as any other disease or condition that has a biomarker such as an antibody or other characterizing protein or biomolecule associated with the disease or progression of the disease. The large ligand libraries of the invention can be used directly in biological fluid, under the appropriate experimental conditions and according to the processes recited herein, to screen for such markers and without the need to use fewer support members (e.g. about 100,000 or less) or without the need to transfer such peptoids or ligands to a microarray before screening the biological fluid. In addition, the ligand libraries may also be used to screen for cell based receptors that specifically relate to a particular cell surface marker. | 10-25-2012 |
20120289415 | METHODS AND PRODUCTS RELATED TO THE IMPROVED ANALYSIS OF CARBOHYDRATES - The invention relates, in part, to the improved analysis of carbohydrates. In particular, the invention relates to the analysis of carbohydrates, such as N-glycans and O-glycans found on proteins and saccharides attached to lipids. Improved methods, therefore, for the study of glycosylation patterns on cells, tissue and body fluids are also provided. Information from the analysis of glycans, such as the glycosylation patterns on cells, tissues and in body fluids, can be used in diagnostic and treatment methods as well as for facilitating the study of the effects of glycosylation/altered glycosylation. Such methods are also provided. Methods are further provided to assess production processes, to assess the purity of samples containing glycoconjugates, and to select glycoconjugates with the desired glycosylation. | 11-15-2012 |
20120302452 | COMPARATIVE LIGAND MAPPING FROM MHC CLASS I POSITIVE CELLS - The present invention relates generally to a methodology for the isolation, purification and identification of peptide ligands presented by MHC positive cells. In particular, the methodology of the present invention relates to the isolation, purification and identification of these peptide ligands from soluble class I and class II MHC molecules which may be uninfected, infected, or tumorgenic. The methodology of the present invention broadly allows for these peptide ligands and their comcomittant source proteins thereof to be identified and used as markers for infected versus uninfected cells and/or tumorgenic versus nontumorgenic cells with said identification being useful for marking or targeting a cell for therapeutic treatment or priming the immune response against infected cells. | 11-29-2012 |
20120316075 | SEQUENCE PRESERVED DNA CONVERSION FOR OPTICAL NANOPORE SEQUENCING - The present invention relates to a method for conversion of a target nucleic acid molecule according to a predetermined nucleotide code into a converted nucleic acid molecule. The converted nucleic acid molecule has utility for determining the nucleotide sequence of the target nucleic acid molecule, for example, using a nanopore. | 12-13-2012 |
20120329661 | Isotope Labeling-Assisted Quantification (iLAQ) of Biological Compounds - The present invention relates to a plurality of isotopically labeled compounds (“tag isotopomers”) and individual labeled compounds, which are useful for labeling samples of analytes, such as biological compounds. The present invention further relates to methods of labeling and quantifying analytes using these tag isotopomers. | 12-27-2012 |
20130017961 | Biomarkers for predicting response of esophageal cancer patient to chemoradiotherapy - The present invention relates to novel genetic markers associated with response of a patient with esophageal cancer (ECa) to chemoradiation therapy, and particularly to methods and kits for predicting an ECa patient's response to chemoradiation therapy by genotyping of the markers. | 01-17-2013 |
20130096016 | METHOD FOR DIFFERENTIALLY QUANTIFYING NATURALLY PROCESSED HLA-RESTRICTED PEPTIDES FOR CANCER, AUTOIMMUNE AND INFECTIOUS DISEASES IMMUNOTHERAPY DEVELOPMENT - The invention relates to a method for quantitatively identifying relevant HLA-bound peptide antigens from primary tissue specimens on a large scale without labeling approaches. This method can not only be used for the development of peptide vaccines, but is also highly valuable for a molecularly defined immunomonitoring and the identification of new antigens for any immunotherapeutic strategy in which HLA-restricted antigenic determinants function as targets, such as a variety of subunit vaccines or adoptive T-cell transfer approaches in cancer, or infectious and autoimmune diseases. | 04-18-2013 |
20130102478 | INTERNAL STANDARDS AND METHODS FOR USE IN QUANTITATIVELY MEASURING ANALYTES IN A SAMPLE - The invention provides methods for quantitatively analyzing a plurality of analytes in a sample. Also described are general and specific internal standards useful in such analysis. In particular embodiments, these standards are described as useful in liquid chromatography/mass spectroscopy systems, which are also described herein. Moreover, in certain embodiments, the quantification methods of the present invention are useful in increasing the precision and/or accuracy of multiple analyte quantification for analytes contained in a single sample mixture using known analyte derivatives simultaneously analyzed, and compared to the unknown analytes. | 04-25-2013 |
20130109578 | DIFFERENTIATION OF ISOBARIC AMINO ACIDS AND OTHER SPECIES | 05-02-2013 |
20130203610 | Tools and Method for Nanopores Unzipping-Dependent Nucleic Acid Sequencing - Provided herein is a library that comprises a plurality of molecular beacons (MBs), each MB having a detectable label, a detectable label blocker and a modifier group. The library is used in conjunction with nanopore unzipping-dependent sequencing of nucleic acids. | 08-08-2013 |
20130225423 | METHOD FOR ENRICHMENT AND ISOLATION OF ENDOGENOUS TRANSCRIPTION FACTOR AND COMPLEXES THEREOF AND CORRESPONDING TANDEM ARRAYS OF CONCATENATED TRANSCRIPTION FACTOR RESPONSE ELEMENTS - The present invention provides a method for enrichment and isolation of endogenous transcription factors and their complexes. Also, this invention provides corresponding tandem arrays of concatenated transcription factor response elements (catTFRE). The method employs the property of transcription factors binding to sequence-specific DNA elements during regulation of gene expression. The catTFREs are designed and synthesized as concatenate dual copies of DNA response elements for various transcription factors. The DNA sequence of synthesized catTFRE is cloned to a target vector. Biotinylated catTFRE with 200 bp arms is prepared by PCR strategy. For enrichment and isolation of endogenous transcription factors and their complexes, the biotinylated catTFRE is immobilized to streptavidin-coated magnetic beads and then incubated with nuclear extract. Thereby endogenous transcription factors and their complexes are isolated from nuclear extract. Identification by mass spectrometry or other functional characterization can be further performed according to the application purposes. | 08-29-2013 |
20130231255 | HIGH MANNOSE GLYCANS - Methods and compositions related to high mannose glycans are described. | 09-05-2013 |
20130296176 | BIOMARKER ANAYLSIS USING SCODAPHORESIS - The invention discloses methods and apparatus for characterizing trace nucleic acids that are biomarkers for disease. The methods and apparatus provide increased sensitivity to such trace nucleic acids, and allow analysis of nucleic acids present in a sample at only 0.01% of the wild-type sequences. The methods and apparatus are also designed for straightforward multiplexing, thus allowing pooling of clinical samples. | 11-07-2013 |
20130310265 | METHODS OF PREPARING CYCLIC PEPTIDES AND USES THEREOF - This invention is directed to the discovery of improved methods of preparing cyclic peptides, cyclic peptide esters, cyclic peptide amidines, and libraries of these compounds. The invention also includes uses of these compounds and libraries for screens as drugs and binders of biologics. | 11-21-2013 |
20140171331 | INTEGRATED ANALYTICAL SYSTEM AND METHOD - An analytical assembly within a unified device structure for integration into an analytical system. The analytical assembly is scalable and includes a plurality of analytical devices, each of which includes a reaction cell, an optical sensor, and at least one optical element positioned in optical communication with both the reaction cell and the sensor and which delivers optical signals from the cell to the sensor. Additional elements are optionally integrated into the analytical assembly. Methods for forming and operating the analytical system are also disclosed. | 06-19-2014 |
20140194301 | ACTIVE CHEMICALLY-SENSITIVE SENSORS WITH IN-SENSOR CURRENT SOURCES - Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions. | 07-10-2014 |
20140194302 | ACTIVE CHEMICALLY-SENSITIVE SENSORS WITH CORRELATED DOUBLE SAMPLING - Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions. | 07-10-2014 |
20140194303 | ACTIVE CHEMICALLY-SENSITIVE SENSORS WITH RESET SWITCH - Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions. | 07-10-2014 |
20140206555 | Nanochannel Arrays and Their Preparation and Use for High Throughput Macromolecular Analysis - Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods. | 07-24-2014 |
20140235463 | METHODS AND APPARATUS FOR MEASURING ANALYTES - Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions. | 08-21-2014 |
20140243217 | METHODS AND COMPOSITIONS FOR DETECTING PLANT EXPOSURE TO PLANT PATHOGENS - The present disclosure provides methods an compositions for the detection of plant exposure to plant pathogens. The methods relate to determining the identity of peptides isolated from the surface of plant starch granules. | 08-28-2014 |
20140287936 | METHODS FOR DRUG DISCOVERY, DISEASE TREATMENT, AND DIAGNOSIS USING METABOLOMICS - The small molecule profiles of cells are compared to identify small molecules which are modulated in altered states. Cellular small molecule libraries, methods of identifying tissue sources, methods for treating genetic and non-genetic diseases, and methods for predicting the efficacy of drugs are also discussed. | 09-25-2014 |
20140329699 | SYSTEMS AND METHODS FOR HIGH EFFICIENCY ELECTRONIC SEQUENCING AND DETECTION - The present disclosure relates to systems and methods for high efficiency electronic sequencing of nucleic acids and molecular detection. In an example embodiment of the instant disclosure, the NanoNeedle may be utilized to detect a change in impedance resulting from the modulation of the counter ion concentration or Debye length associated with a biomolecule of interest, such as DNA or protein, for an application of interest, such as DNA sequencing, DNA hybridization, or protein detection. | 11-06-2014 |
20140357501 | METHOD AND SYSTEM FOR ANALYZING PROTEIN OR PEPTIDE - A peptide is cleaved at various bonding sites into oligopeptides or similar fragments by digestion using proteinase K (S | 12-04-2014 |
20140357502 | Windowing Combined with Ion-Ion Reactions for Chemical Noise Elimination - In a first location of a mass spectrometer, a plurality of ionized molecules of an ion source are selected that have mass-to-charge ratios within a mass-to-charge ratio window width. The plurality of selected ionized molecules are transmitted from a first to a second location. Reagent ions are transmitted to the second location to reduce a charge state of one or more of the plurality of selected ionized molecules. A mass analyzer is used to analyze the plurality of reduced ionized molecules and produce a mass spectrum. A compound is identified from a peak of the spectrum that has a mass-to-charge ratio less than or equal to the highest mass-to-charge ratio in the window width if the noise is multiply charged and greater than the highest mass-to-charge ratio in the window width if the noise is singly charged. | 12-04-2014 |
20140364324 | NANOPORE SEQUENCING USING CHARGE BLOCKADE LABELS - The invention relates to devices and methods for nanopore sequencing. The invention includes compositions and methods of nucleic acid sequencing using a single polymerase enzyme complex comprising a polymerase enzyme and a template nucleic acid attached proximal to a nanopore, and nucleotide analogs in solution comprising charge blockade label that are attached to the polyphosphate portion of the nucleotide analog such that the charge blockade labels are cleaved when the nucleotide analog is incorporated into a growing nucleic acid and the charge blockade label is detected by the nanopore to determine the presence and identity of the incorporated nucleotide and thereby determine the sequence of a template nucleic acid. | 12-11-2014 |
20140378323 | METHOD OF NUCLEOTIDE DETECTION - The invention relates to an additive which can be added to buffers used in nucleotide detection processes and improved methods of nucleic acid sequencing using this additive. In particular the invention relates to use of the additive to improve the efficiency of fluorescence-based multiple cycle nucleic acid sequencing reactions. | 12-25-2014 |
20150045238 | BIOMARKERS FOR DIABETES - The present invention provides a method for assessing the presence and risk of developing type 2 diabetes, cancer of all sites, or cardiovascular disease in a subject by detecting sequence variation in one or more genes such as carboxypeptidase E (CPE) and insulin degradation enzyme (IDE). A kit, array, and device useful for such a method are also provided. In addition, the present invention provides a method for treating type 2 diabetes, cancer of all sites, or cardiovascular disease in patients who have been tested and shown to have the pertinent genetic variations. | 02-12-2015 |
20150094214 | SYSTEMS AND METHODS FOR CHARACTERIZING A MOLECULE - Techniques for characterizing a molecule are described herein. In one example, a portion of the molecule is trapped in a nanopore, a variable voltage is applied across the nanopore until the trapped portion of molecule is moved within the nanopore, and the molecule is characterized based on the electrical stimulus required to affect movement of at least a portion of the trapped portion of the molecule within the nanopore. | 04-02-2015 |
20150141271 | METHOD FOR ANALYZING WATER TOXICITY - A method for analyzing water toxicity, the method including: exposure experiment, sample collection, transcriptome detection, metabolome detection, screening of differentially expressed genes, screening of differentially expressed metabolites, and identification of commonly changed biological pathways in both the transcriptome and the metabolome. | 05-21-2015 |
20150293074 | METHOD OF ANALYSIS OF GENETIC MARKERS - A method of analyzing genetic markers includes binding a set of probes to a segment of single stranded nucleic acids. The segment of single stranded nucleic acids includes a repeat region formed of at least two of a repeat unit. The repeat unit can include at least two nucleic acids. The set of probes includes a first probe complementary to the repeat unit. The method can further include directing the segment through a nanopore device and measuring a signal through the nanopore device. The signal can be indicative of the number of repeat units. | 10-15-2015 |
20150299810 | Methods for Detecting Signatures of Disease or Conditions in Bodily Fluids - This invention provides methods of using cell free bodily fluid and blood cells in the diagnosis, prognosis, or monitoring of diseases or conditions. The invention also relates to methods of using cell free bodily fluid and blood cells to identify markers of diseases or conditions. | 10-22-2015 |
20150300968 | Device and Methods for Analysis of Biomolecule Structure, Dynamics and Activity - The present invention provides a method for determining the 3-dimensional or 3-D atomic resolution structure of a biomolecule using chemical shift data obtained from the biomolecule having one or more selectively isotopically labeled biomolecule monomers and interrogation of same using an NMR device. Methods also comprise use of a low-field NMR device and structure determination method to determine the 2-D and 3-D structure of the biomolecule, for example, a polynucleotide. | 10-22-2015 |
20150301002 | AUTOMATED SYSTEM FOR SAMPLE PREPARATION AND ANALYSIS - A sample preparation and analysis system. The system includes a sample preparation system and a sample analysis system. The sample preparation system prepares samples in accordance with an assay that is selected from a database containing a plurality of unique assays. The sample analysis system includes an analyzer that is dynamically reconfigurable based on the selected assay so as to analyze the prepared sample in accordance with that selected assay. A data communication link communicates data from the sample preparation system to the sample analysis system to reconfigure the analyzer in accordance with the selected assay. | 10-22-2015 |
20150307931 | CHARACTERIZATION OF INDIVIDUAL POLYMER MOLECULES BASED ON MONOMER-INTERFACE INTERACTIONS - The invention relates to a method for detecting a double-stranded region in a nucleic acid by (1) providing two separate, adjacent pools of a medium and a interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage of a single-stranded nucleic acid, but not of a double-stranded nucleic acid, from one pool to the other pool; (2) placing a nucleic acid polymer in one of the two pools; and (3) taking measurements as each of the nucleotide monomers of the single-stranded nucleic acid polymer passes through the channel so as to differentiate between nucleotide monomers that are hybridized to another nucleotide monomer before entering the channel and nucleotide monomers that are not hybridized to another nucleotide monomer before entering the channel. | 10-29-2015 |
20150307949 | IDENTIFICATION AND ERADICATION OF INSECT PESTS - A method of identifying the biotype (or clade) of a whitefly comprising using at least one of: (a) an hsp90 gene, polypeptide or protein, (b) an enolase gene, polypeptide or protein, (c) a fructose bisphosphate aldolase gene, polypeptide or protein, (d) a triose phosphate isomerase gene, polypeptide or protein, or (e) a vitellogenin gene, polypeptide or protein, of the whitefly. A kit for identifying | 10-29-2015 |
20150309045 | METHOD AND TOOLS FOR THE DETERMINATION OF CONFORMATION AND CONFORMATIONAL CHANGES OF PROTEINS AND OF DERIVATIVES THEREOF - A Method for the detection of the conformational state of a protein contained in a complex mixture of further proteins and/or other biomolecules, is proposed as well as assays for such a method. The method comprises the following steps: (1) Limited proteolysis of the complex mixture under a condition where the protein is in the conformational state to be detected leading to a first fragment sample; (2) Denaturation of the first fragment sample to a denaturated first fragment sample; (3) Complete fragmentation of the denaturated first fragment sample in a digestion step to a completely fragmented sample; and (4) Analytical analysis of the completely fragmented sample for the determination of fragments characteristic of having been the result both the limited proteolysis of step 1 as well as of the complete fragmentation in the digestion step 3. for the determination of the conformational state. | 10-29-2015 |
20150310169 | METABOLIC PROFILING IN TISSUE AND SERUM IS INDICATIVE OF TUMOR DIFFERENTIATION IN PROSTATE CANCER - The invention provides methods and products to detect the presence of unidentified high grade prostate tumors in a subject with a Gleason score 7 prostate tumor. The method comprises obtaining a biological sample from a subject in need thereof, measuring a profile of metabolites in the biological sample, wherein the metabolites are differentially expressed in Gleason score 6 versus Gleason score 8 prostate tumors, and classifying the profile of the metabolites to assign a grade to the sample based on the profile of the metabolites. | 10-29-2015 |
20150316562 | METHOD AND BIOMARKERS FOR THE DETECTION OF DENGUE HEMORRHAGIC FEVER - The present invention provides methods for detecting, analyzing, and identifying biomolecules used to identifying patient with dengue-like symptom who are at risk of DHF. The inventive method comprises detecting in a sample from a subject dengue infected patient one or more biomarkers selected from the group consisting of IL-10, fibrinogen, C4A, immunoglobulin, tropomyosin, and three isoforms of albumin, and which are used in a predictive MARS model to detect patients with risk of developing DHF. | 11-05-2015 |
20150322505 | BARRIER COATED NANO STRUCTURES - The present invention relates to a device comprising a nano-structure and a corresponding method of manufacturing, wherein said nano-structure is made of electrically conductive material and wherein said nano-structure is covered by a barrier coating comprising Ti, Zr, Hf, Nb, Ta, Mo, Sc, Y, Ge, La, Ce, Pr, Nd, Sm, Eu, Gd, Dy, Ho, Er, Tm, Yb, Lu, Sr, Al, B, Ba, Bi, and/or Mg oxide in a thickness of at least about 1 nm, wherein said barrier coating is deposited by atomic layer deposition (ALD). The present invention also relates to a method of detecting a target compound in such a device, the use of such a device for surface specifically creating an evanescent field, measuring the dielectric properties of a medium, detecting the presence or the concentration of a target compound, determining the primary structure of a target compound, determining a deviation of the target compound from a control value, amplifying a target compound, or monitoring the amplification of a target compound. | 11-12-2015 |
20150323518 | NANONOZZLE DEVICE ARRAYS: THEIR PREPARATION AND USE FOR MACROMOLECULAR ANALYSIS - Constricted nanochannel devices suitable for use in analysis of macromolecular structure, including DNA sequencing, are disclosed. Also disclosed are methods for fabricating such devices and for analyzing macromolecules using such devices. | 11-12-2015 |
20150329905 | SENSOR ARRAYS AND NUCLEIC ACID SEQUENCING APPLICATIONS - Embodiments of the present invention provide devices methods for sequencing DNA using arrays of reaction cavities containing sensors to monitor changes in solutions contained in the reaction cavities. Additional embodiments provide devices and methods for sequencing DNA using arrays of reaction cavities that allow for optical monitoring of solutions in the reaction cavities. Test and fill reaction schemes are disclosed that allow DNA to be sequenced. By sequencing DNA using parallel reactions contained in large arrays, DNA can be rapidly sequenced. | 11-19-2015 |
20150344944 | ANALYSIS OF A POLYNUCLEOTIDE VIA A NANOPORE SYSTEM - A target polynucleotide is expanded. In respect of each nucleotide in the target polynucleotide, the target polynucleotide comprises clock nucleotides and at least one signal nucleotide in a predetermined order. The clock nucleotides have a predetermined sequence common to each nucleotide in the target polynucleotide. The at least one signal nucleotide is characteristic of the identity of the respective nucleotide in the target polynucleotide. During translocation of the expanded polynucleotide through a nanopore, electrical measurements dependent on the polynucleotide within the pore are made, to derive an analysis signal. Clock signals derived from the clock nucleotides are identified. Relative to the positions of the identified clock signals, nucleotide signals derived from the least one signal nucleotide are derived to analyse the target polynucleotide. The predetermined sequence of the clock nucleotides comprises a restriction site for a restriction enzyme and at least one further nucleotide that extends the predetermined sequence. | 12-03-2015 |
20150346197 | APPARATUS, SYSTEMS AND METHODS FOR SENSING AN ANALYTE SUCH AS ETHANOL - According to one aspect, a system for sensing ethanol from human skin, that comprises at least one apparatus for sensing ethanol and a coupling matrix readout extraction unit for performing a method of extracting a coupling-matrix readout. The at least one sensing apparatus and the coupling matrix readout extraction may be mounted on a substrate. The substrate may be adapted such that it can be formed into a wearable accessory that can be worn by a human subject. When the wearable accessory is worn by the human subject such that the subject provides a skin tissue sample to the accessory, the presence or concentration of ethanol and other compounds may be determined from the vapour associated with the tissue sample. | 12-03-2015 |
20150361488 | Sequencing Device - A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening. | 12-17-2015 |
20150362507 | System and Methods of Detecting and Demonstrating Ultraviolet Damage to Hair Via Evaluation of Protein Fragments - The present invention is directed to a method to measure ultraviolet or copper damage of hair comprising: eluting a protein fragment from a hair sample with an aqueous solution; extracting the proteins using a solvent; analyzing the protein fragment samples with MALDI-MS; resulting in protein fragment results; identifying presence of a marker protein fragment and identifying what the fragment is by indentifying the amino acid sequence using high resolution Orbitrap-MS wherein the protein fragment is a protein fragment of S100A3. | 12-17-2015 |
20150368707 | SYSTEMS AND METHODS FOR AUTOMATED REUSABLE PARALLEL BIOLOGICAL REACTIONS - A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions. | 12-24-2015 |
20160002696 | METHOD TO IDENTIFY BACTERIAL SPECIES BY MEANS OF GAS CHROMATOGRAPHY/MASS SPECTROMETRY IN BIOLOGICAL SAMPLES - Method to identify bacterial classes in a biological sample, in particular a urine sample, that provides to carry out an analysis by means of Gas Chromatography-Mass Spectrometry (GC/MS) of the volatile components, such as metabolites and catabolites, of the sample in order to identify a graphic plot characteristic of a specific bacterial class. | 01-07-2016 |
20160003857 | METHODS AND SYSTEMS FOR DETECTING POLYPHARMACY - A method is disclosed for detecting polypharmacy in a subject that involves assaying a urine sample from the subject for a plurality of drugs or drug metabolites. The disclosed methods can further involve detecting potential adverse drug reactions in the polypharmacy of the subject. In addition, the method can further involve selecting a drug for treating the subject that does not interact with any drugs in the polypharmacy of the subject. | 01-07-2016 |
20160025740 | PROTEIN COMBINATION-BASED FV LIBRARY, AND PREPARATION METHOD THEREFOR - The present invention relates to a method for constructing an Fv library based on a combination of proteins, a method of screening a desired antibody using the constructed Fv library, an Fv antibody screened by the screening method, and an Fv library constructed by the Fv library construction method. The Fv library of the present invention is based on a combination of proteins so that members thereof can be individually analyzed for their function. Moreover, the Fv library enables a desired Fv antibody to be screened without needing a target antigen preparation. In addition, the protein combination based Fv library makes it possible to significantly reduce the number of protein purification processes to thereby reduce costs and time, compared to conventional DNA-based libraries. | 01-28-2016 |
20160032380 | ELECTRON BEAM NUCLEIC ACID SEQUENCING - The present invention relates to compositions, methods, and uses for obtaining sequence information from nucleic acid molecules. | 02-04-2016 |
20160130650 | NANOWIRE-BASED SYSTEM FOR ANALYSIS OF NUCLEIC ACIDS - System for detection and/or analysis of nucleic acids using nanowires to detect covalent modification of nucleic acids. | 05-12-2016 |
20160161413 | SYSTEM AND METHOD FOR DETECTION OF CONTAMINANTS - Described is a system and method for detecting contaminants using a flexible Surface Enhanced Raman Spectroscopy (SERS) substrate. The contaminant is collected on a flexible SERS substrate and directly interrogated with a Raman Spectrometer to obtaining a SERS emission spectrum for the contaminant. The obtained spectrum can be compared to a library of SERS signatures to identify the contaminant. | 06-09-2016 |
20160187332 | SYSTEMS AND METHODS FOR DETECTING A SUBSTANCE IN BODILY FLUID - Various devices, systems and methods for determining a parameter of and/or detecting chemical and biological substances in bodily fluid are described herein. A device or system may include a substrate. An active sensor having an electrical characteristic and/or a control sensor may be disposed on the substrate. In certain variations, a differential between a first signal from the active sensor, and a second signal from the control sensor may be used to determine a parameter of the chemical or biological substance in the sample of bodily fluid. | 06-30-2016 |
20160195486 | Method and Apparatus for Detection, Identification and Quantification of Chemical or Biological Material through Modification of Operating Conditions | 07-07-2016 |
20160195503 | PROTEIN-RICH MICROALGAL BIOMASS COMPOSITIONS OF OPTIMIZED SENSORY QUALITY | 07-07-2016 |
20180023136 | Compositions, Devices, Systems, and Methods for Using a Nanopore | 01-25-2018 |
20180023137 | Compositions, Devices, Systems, and Methods for Using a Nanopore | 01-25-2018 |