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With fluorescence or luminescence

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436 - Chemistry: analytical and immunological testing

436164000 - OPTICAL RESULT

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DocumentTitleDate
20110177609DEVICE AND METHOD FOR PRESSURE-DRIVEN PLUG TRANSPORT - The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.07-21-2011
20080261324System, Device and Method for Reducing Luminescence Output Shifts - Devices, systems, and methods for conducting chemiluminescent immunoassay testing and, more particularly, to initiating and monitoring a chemiluminescent reaction in a plurality of such assays, of different types, on a single immunoassay instrument, in a single procedure, using a plurality of labels and a triggering reagent combination are disclosed. Moreover, by including a base reagent injector assembly having an “e-channel” to provide a swirling turbulence to the base reagent immediately before it is introduced into the well of a cuvette containing a sample and an acid reagent. The added turbulence addresses the phenomenon referred to as “RLU shift,” in which the luminescence output can increase or decrease between assays.10-23-2008
20110195516WIRE GRID SUBSTRATE STRUCTURE AND METHOD FOR MANUFACTURING SUCH A SUBSTRATE - The present invention relates to a multi-layered substrate structure comprising at least one carrier layer (08-11-2011
20100112716SYNTHESIS OF NANOASSEMBLIES CONTAINING LUMINESCENT QUANTUM DOTS AND MAGNETIC NANOPARTICLES - Negatively charged luminescent CdSe—ZnS quantum dots (QDs) were successfully incorporated into novel luminescent glyconanospheres averaging around 190 nm in diameter through electrostatic interactions with carboxymethyldextran (CM-dextran) and polylysine. The glyconanospheres preferably contain as well carboxyl-modified iron oxide nanocrystals. In addition to electrostatic attraction between the negatively charged dextran, the negatively charged CdSe—ZnS QDs (and negatively charged iron oxide nanocrystals, if present), and the positively charged polylysine, covalent amide bonds were introduced to cross link the QDs (and negatively charged iron oxide nanocrystals, if present) with the polysaccharide matrix to further stabilize the glyconanospheres. The dextran residues on the surface of the nanospheres show high affinity toward the glucose binding protein-Concanavalin A (Con A). As a result, these luminescent CdSe—ZnS QD incorporated glyconanospheres are a useful tool for studying carbohydrate-protein interactions that are critical steps in bacterial and viral infection.05-06-2010
20130078730METHOD FOR MONITORING AND CONTROL OF A WASTEWATER PROCESS STREAM - The invention is directed towards methods, compositions, and apparatus for accurately detecting the presence and amounts of contaminants in wastewater. The method comprises the steps of adding to a volume of wastewater at least one tracer molecule, observing the tracer for indications of particular contaminants, conducting at least one second form of contamination detection, and interrelating the two measured properties to identify the specific composition of the contamination. Using a tracer molecule allows for the detection of otherwise hard to detect oils and grease. Use of the second method however compensates for tracer interfering contaminants and allows for more accurate readings. The invention includes feeding of functional chemicals in response to the detections and conducting the detections online and continuously.03-28-2013
20100075434Method for detecting target substance, and tag, DNA, vector, probe and detection kit for use therewith - Provide is a method for detecting a target substance, which method can visualize the expression of the target substance at any time point while reducing influences on the functions of the target substance, can use fluorescent dyes having various excitation/emission wavelengths, and can achieve easy staining process. Also provided are a tag, a DNA, a vector, a probe and a detection kit suitable for use in the above-described detection method. Specifically, the method for detecting a target substance, comprises the steps of bringing into contact with each other (a) a tag comprising a polypeptide forming an α-helix structure, the tag bound to the target substance, and (b) a probe comprising a compound bound to a fluorescent dye; and measuring the fluorescence emitted by the fluorescent dye. The binding of the tag α-helix structure to the probe compound induces a spectral change in the fluorescence emitted by the fluorescent dye.03-25-2010
20130078731CHROMOPHORES FOR THE DETECTION OF VOLATILE ORGANIC COMPOUNDS - Aromatic diimide chromophores and methods for using the chromophores for the detection of volatile organic compounds are described. The chromophores are able to reversibly change colors in the presence or absence of volatile organic compounds.03-28-2013
20130034912CENTRIFUGAL FORCE-BASED MICROFLUIDIC DEVICE AVAILABLE FOR RELIABILITY VERIFICATION, AND ANALYZING METHOD USING THE SAME - A centrifugal force-based microfluidic device for a multiplexed analysis and an analyzing method using the same are provided. The microfluidic device includes a platform and a microfluidic structure including a plurality of chambers formed within the platform, and valves positioned between the chambers. The microfluidic structure includes a sample separation chamber connected to a sample injection hole, and a plurality of reaction chambers accommodating two or more types of markers specifically reacting with different types of target materials, separately by type. At least one of the target materials is a standard material, and at least one of the markers is a standard marker specifically reacting with the standard material.02-07-2013
20130034913Method for Investigating a Specimen Containing Fluorescing Dyes with the Aid of a Microscope - In order to investigate a specimen (02-07-2013
20100105149FLUORESCENT DYE-LABELED GLUCOSE BIOPROBE, SYNTHESIS METHOD AND USAGE THEREOF - The present invention relates to a fluorescent dye-labeled glucose analog, and a synthesis method and usage of the same, and more particularly, to novel glucose α and β anomers in which a fluorescent dye is labeled by O-1-glycosylation, an asymmetric synthesis method of the anomers, a molecular bioimaging method of the anomers, and a screening method of curing or preventing drugs for diseases related to glucose metabolism.04-29-2010
20100068822Device For Carrying Out Tests On And Analyzing Biological Samples With Temperature-Controlled Biological Reactions - The invention relates to a device for carrying out tests on and analyzing biological samples with temperature-controlled biological reactions. It comprises: A reaction chamber (03-18-2010
20090042312Test method for determining compounds capable of quenching electronic singlet state excitation of photoactive compounds - A method of testing a compound for its capability of quenching singlet excited state energy from a photon-excited photoactive compound, thereby photostabilizing said photoactive compound, comprising mixing: (1) a composition comprising a photoactive compound in a solvent and determining the degree of fluorescence of said composition (1) by exposing the composition (1) to UV radiation in an amount sufficient for the photoactive compound to reach an electronic singlet excited state, and (2) a mixture comprising said photoactive compound with a test compound in a solvent, and determining the degree of fluorescence of said mixture (2) by exposing the mixture to UV radiation to the same degree as composition (1), and comparing the degree of fluorescence of composition (1) with mixture (2).02-12-2009
20130071943SURFACE WITH TWO PAINT STRIPS FOR DETECTION AND WARNING OF CHEMICAL WARFARE AND RADIOLOGICAL AGENTS - A system for warning of corrosion, chemical, or radiological substances. The system comprises painting a surface with a paint or coating that includes an indicator material and monitoring the surface for indications of the corrosion, chemical, or radiological substances.03-21-2013
20130164857MEASURING CASSETTE AND MEASURING DEVICE FOR THE DETECTION OF TARGET MOLECULES IN A LIQUID SAMPLE BY MEASUREMENT OF FLUORESCENCE EMISSION AFTER EXCITATION IN AN EVANESCENT FIELD - An interchangeable disposable measuring cassette for insertion into a measuring apparatus for detecting target molecules in a liquid sample by measuring fluorescence emission has a flow measurement cell in which an excitation radiation provided by the measuring apparatus produces an evanescent field in the liquid sample beyond a boundary layer for the liquid sample and the measurement cell. To be better able to ensure that no sample liquid can cross from the measurement cell into the measuring apparatus, the measuring cassette includes a body including an optically transparent material and a base in contact with the underside of the body. The measurement cell is formed by a cutout provided in the body, the base, or both. The areas on which the body and the base are on top of one another around this cutout are connected to one another directly and in fluid-tight fashion by laser welding.06-27-2013
20130059398USE OF NOVEL MATERIALS IN MARKER SYSTEMS - The present invention relates to the use of marker systems for the security and tracing of items, articles, goods, vehicles or persons. The present invention provides a marker for applying to the surfaces of items, the marker comprising at least one fluorescent material capable of fluorescing at a specific wavelength when subjected to stimulus. The marker can also include at least one material which is phosphorescent and emitting at a specific wavelength. In use, the measured fluorescence and phosphorescent response obtained from the marker are visually compared to examples of the desired colour output for the case in hand to verify the authenticity of the item.03-07-2013
20110014721HYDROPHILIC CHEMILUMINESCENT ACRIDINIUM LABELING REAGENTS - In accordance with the present invention, it has been discovered that introduction of hydrophilic sulfoalkyl substituents and/or hydrophilic linkers derived from homocysteic acid, cysteic acid, glycine peptides, tetraethylene oxide, and the like, offset the hydrophobicity of the acridinium ring system to produce a more soluble label which can be attached to an antibody at higher loading before precipitation and aggregation problems are encountered. Additional compounds described herein contain linkers derived from short peptides and tetraethylene oxide which increase aqueous solubility due to hydrogen bonding with water molecules. The present invention also embraces reagents for multiple acridinium labeling for signal amplification composed of a peptide bearing several acridinium esters with sulfonate groups at regularly spaced intervals for increased solubility. The invention also embraces assays employing the above-described compounds.01-20-2011
20110014720METHOD FOR HOMOGENEOUS BIOLOGICAL ASSAY - A method for performing time resolved homogeneous assays using a long-lifetime luminescent dye as a donor. A reaction well containing a sample portion, donor reagent, and acceptor reagent and a matrix well containing a sample portion and donor reagent are excited and the resulting emission from each is measured at a single wavelength associated with the acceptor. The measurement obtained from the matrix well is used to provide a correction for the measurement obtained from the reaction well. The sample may be a biological fluid such as an oral fluid.01-20-2011
20110020946CONVERSION OF JUST-CONTINUOUS METALLIC FILMS TO LARGE PARTICULATE SUBSTRATES FOR METAL-ENHANCED FLUORESCENCE - The present invention relates to a method of producing silver films having large nanoparticles caused by cracking during anaerobic annealing to provide surfaces that exhibit increased metal enhanced fluorescence. Preferably the annealing process is conducted on a silver film having a thickness from about 14 to 17 nm for about an hour at a temperature of approximately 190° C. to about 210° C. resulting in the conversion of the just-continuous films into large particulate films, not readily assessable by other chemical deposition techniques.01-27-2011
20120115241SYSTEMS, DEVICES, AND METHODS FOR REMOTELY INTERROGATED CHEMOSENSOR ELECTRONICS - Systems, devices and methods for remotely interrogating sensor electronics are described. In one embodiment, a system for detecting and localizing chemical analytes is described. This system includes a plurality of chemosensor electronic devices for detecting the presence of chemical analytes. Each of these devices includes a chemosensor for sensing chemical analytes, a transponder, and an electronic circuit for activating the transponder based on an output of the chemosensor. These devices may have a cross-section area of less than 1 square micrometer. The system also includes an interrogation device for interrogating the plurality of devices and for receiving information on the detected chemical analytes from devices with activated transponders, and a processor for determining the locations of the devices with activated transponders. These locations may be forwarded to a third party.05-10-2012
20090311799Nucleic Acid Materials for Nonradiative Energy Transfer and Methods of Production and Use - Nucleic acid materials for FRET-based luminescence and methods of making and using the nucleic acid materials are provided. The nucleic acid materials provide an innovative and synergistic combination of three disparate elements: a nucleic acid material, the processing technique for forming a nucleic acid material into films, fibers, nanofibers, or non-woven meshes, and nonradiative energy transfer. This combination can be formed into electrospun fibers, nanofibers, and non-woven meshes of a nucleic acid material-cationic lipid complex with encapsulated chromophores capable of nonradiative energy transfer such as efficient Förster Resonance Energy Transfer (FRET).12-17-2009
20090011518Device and Method for Detection of Fluorescence Labelled Biological Components - A sample acquiring device for detection of biological components in a liquid sample is provided comprising a measurement cavity for receiving a liquid sample, wherein the measurement cavity has a predetermined fixed thickness, and a reagent, which is arranged in a dry form inside the measurement cavity. The reagent comprises a fluorophore conjugated molecule.01-08-2009
20100003765METHODS AND SYSTEMS FOR MITIGATING OXYGEN ENHANCED DAMAGE IN REAL-TIME ANALYTICAL OPERATIONS - Methods and systems for performing analytical reactions under reduced or non-oxygen conditions, where such reactions are potentially subject to damaging effects of oxygen, including particularly fluorescent based detection methods where fluorescent species may be prone to generation of reactive oxygen species.01-07-2010
20080268548Enhancing Raman spectrographic sensitivity by using solvent extraction of vapor or particulate trace materials, improved surface scatter from nano-structures on nano-particles, and volumetric integration of the Raman scatter from the nano-particles' surfaces - This invention is a method to enhance by orders of magnitude accurate, real-time, stand-off detection by a sensor using Raman spectra of one or more trace compounds of interest (particularly explosives, bioterror organisms, or Volatile Organic Compounds). A colloid, whose medium of suspension is a liquid solvent with a weak Raman spectrum and in which are suspended particles of a noble metal that are preferentially nano-sized to maximize the surface-to-mass ratio for each particle, forms an impingement base. A sample of this colloid is air-pumped through a sampling module, exposed to air potentially carrying trace molecules from the compound of interest, then sent to a detection module that subjects the sample to Raman spectroscopy. The result is first corrected to obtain a unique Raman spectra from the trace molecules, then matched against Raman spectra in a database. Extensions include modifying, flushing, further processing, or recirculating the colloid sample.10-30-2008
20090068755Optical biosensing platform utilizing nanocrystalline zinc oxide - An optical biosensing platform for the real-time detection of the occurrence of a binding event, the optical biosensing platform comprising a nanocrystalline zinc oxide (nano-ZnO) substrate having a surface and being capable of emitting photoluminescence and a surface modifier formed integral with at least a portion of the surface of the nano-ZnO substrate, wherein the surface modifier is capable of binding to a biomolecule and wherein when the surface modifier binds with a biomolecule, a change is induced in the emitted photoluminescent properties of the nano-ZnO substrate, thereby enabling the detection of a binding event.03-12-2009
20090325308METHOD OF DETECTING RESIDUAL DETERGENT AND DEVICE FOR DETECTING RESIDUAL DETERGENT - It is intended to provide a method whereby residual detergent (surfactant) can be conveniently and accurately detected in the step of washing or rinsing dishes or clothes, and a test device usable in examining the rinsed conditions. Namely, a method characterized by comprising bringing a test substance with a composition (test agent) comprising an oxalic acid ester, a fluorescent substance, hydrogen peroxide and a strong acid and thus easily detecting the residual detergent sticking to the surface of the test subject from the chemiluminescence thus caused; and a test device which is most suitable for storing the test agent as described above.12-31-2009
20100120165Method and System for Manufacture and Use of Macroporous Beads in a Multiplex Assay - Macroporous beads and a method of manufacturing and using such macroporous beads. wherein the beads are distinguishable for use in a multiplex assay. Preferably, the beads are distinguishable by two or more unique fluorochromes, and at least some of the beads are magnetically responsive. In a preferred form, some of the macroporous beads have interior pores with a different moiety from the exterior surface, allowing beads with different attached functional groups.05-13-2010
20090137059Inorganic Polymers and Use of Inorganic Polymers for Detecting Nitroaromatic Compounds - A method for detecting an analyze that may be present in ambient air, bound to a surface or as part of complex aqueous media that includes providing a metallole-containing polymer or copolymer, exposing the polymer or copolymer to a suspected analyze or a system suspected of including the analyze, and measuring a quenching of photoluminescence of the metallole-containing polymer or copolymer exposed to the system. Also included is a solid state inorganic-organic polymer sensor for detecting nitroaromatic compounds that includes a substrate and a thin film of a metallole-containing polymer or copolymer deposited on said substrate.05-28-2009
20100311184AZO HETEROCYCLIC DYES AND THEIR BIOLOGICAL CONJUGATES - Azo heterocyclic compounds that are substantially non-fluorescent or only weakly fluorescent useful as energy acceptors. The chemically reactive compounds possess utility for labeling a wide variety of substances, including biomolecules with the resulted conjugates being highly useful for a variety of energy-transfer assays and applications. The biological conjugates of the disclosure exhibit little or no observable fluorescence and their fluorescence is significantly increased upon a biological stimulation.12-09-2010
20110294225Method for Detecting Optical Signals, Microfluidic Mixing Chip Having Light Emitting Compound and System Thereof - A method for detecting optical signals, a microfluidic mixing chip having light emitting compound and a system thereof are provided. The microfluidic mixing system comprises the microfluidic mixing chip, an electrode pairs and a power supplier. The microfluidic mixing chip comprises a first side cavity, a second side cavity and a mixing cavity. The mixing cavity is disposed between the first side cavity and the second side cavity. The mixing cavity further contains the light emitting compound, a catalyst and a redox reagent. The electrode pair is respectively disposed to the first side cavity and the second cavity. The power supplier supplies a power source with high frequency alternating current electric field. By utilizing the power source with alternating current electric field, the light emitting compound, the redox reagent and the catalyst are mixed in the mixing cavity to generate a chemiluminescence or bioluminescence optical signal to detect.12-01-2011
20090104711FLUORESCENT MARKER COMPRISING DOUBLE BOND ESTER GROUP AND METHOD FOR MARKING AND DETECTING THE SAME - Disclosed herein are fluorescent markers having a double bond ester group and a method for marking and detecting the same. More particularly, disclosed are a method for identifying oil products, which comprises marking the oil products with fluorescent markers having an unsaturated double bond ester group, adding a developing agent having a function of inducing specific fluorescence to the marked oil products, and detecting the fluorescent marker with a fluorescence spectrophotometer in the UV/VIS region, as well as said markers.04-23-2009
20100304497FLUORESCENT PROTEIN AND CHROMOPROTEIN - It is an object of the present invention to provide a novel chromoprotein and a novel fluorescent protein. The present invention provides chromoproteins derived from 12-02-2010
20080206886Large stoke shift dye used for optical imaging - The present invention relates to a diagnostic contrast agent comprising a water dispersible, near-infrared tricarbocyanine, enamine-functionalized dye having a Stoke shift of greater than 50 and represented by five general formulae. The present invention also relates to a method for making a dye-conjugate utilizing the novel dye and a method of identifying a biological compound using the novel dye conjugate.08-28-2008
20090181467Method of assessing and of activating the alternative cellular energy (ACE) pathway in the therapy of diseases - Alternative cellular energy pigments (ACE-pigments) provide a source of cellular energy other than that provided through the oxidative metabolism of foods, or in the case of plants and certain bacteria, through the process of photosynthesis. In some patients, ACE pigments exist in a form that can be further energized or activated using ultraviolet (UV) light, especially if the reaction is initially triggered by the presence of suitable dyes, such as neutral red. A method is described to assess the energy status of the ACE pathway in an individual or animal and also a method that involves the use of UV light in conjunction with a suitable dye, for activating the pathway in those individuals or animals in which the pathway is not fully charged. Kits for these purposes are also described.07-16-2009
20090221088Fluorescent Sensor and Methods - Binding an analyte can cause a change in fluorescence emission of a sensor. The change in fluorescence can be related to the amount of analyte present. The sensor can include a semiconductor nanocrystal linked to a fluorescent moiety. Upon excitation, the fluorescent moiety can transfer energy to the semiconductor nanocrystal, or vice versa.09-03-2009
20120107950MEASUREMENT SYSTEM FOR FLUORESCENT DETECTION, AND METHOD THEREFOR - In one aspect, the invention provides a measurement system, wherein the measurement system comprises a sample module to receive a sample, wherein the sample module comprises at least one fluorophore; an optics module to generate an incident beam to impinge on the sample to yield a laser spot; a detector module to detect fluorescence signals arising out of the sample; a processor module to process the fluorescence signals and provide relevant output; an output module; a control module to control the sample module, the optics module, the detector module and the output module. The measurement system of the invention is capable of measuring both bulk and event fluorescences of a sample containing at least one fluorophore. In another aspect, the invention provides a method for testing a fluid based on the measurement system of the invention.05-03-2012
20110151576METHOD TO ASSESS MULTIPHASE FLUID COMPOSITIONS - A method for the assessment of a multiphase (aqueous and organic) sample phase, the method comprising adding at least one detection molecule to the multiphase sample; detecting a signal emitted from the detection molecule/multiphase sample mixture, the signal being detectably different when the at least one detection molecule is present in one of either an organic phase, an aqueous phase or an interface between said organic phase and said aqueous phase of the multiphase sample; and analysing the detected signal to assess the properties of a phase or an interface between phases. A system for use in such a method, use of at least one detection molecule for the assessment of a multiphase sample, and a composition for use in the assessment of a multiphase sample are also disclosed.06-23-2011
20120034702METHOD AND APPARATUS FOR MEASURING FLUORESCENCE IN LIQUIDS - A method of measuring the fluorescence of a fluorescent marker compound dissolved or dispersed in a bulk material includes: (a) measuring a characteristic of the fluorescence of a mixture of said bulk material and said fluorescent marker compound; (b) quenching the fluorescence of the fluorescent marker compound to produce a quenched mixture; (c) measuring the characteristic of the fluorescence of the quenched mixture; (d) comparing the fluorescent characteristic of the mixture with the fluorescent characteristic of the quenched mixture; and (e) correcting the measured fluorescent emission characteristic for the effects of the absorbance of the bulk material. The measurement may be further corrected to account for the absorbance of the material which is also known to have an effect on the measured fluorescence. A method of tagging and identifying a bulk material with a fluorescent marker compound, and an apparatus for carrying out the methods are also described.02-09-2012
20100124788METHOD FOR HIGH SPATIAL RESOLUTION EXAMINATION OF A SAMPLE STRUCTURE LABELED WITH A SUBSTANCE - A method for high spatial resolution examination of a sample structure includes providing a biological structure as the sample structure with a substance capable of being converted from a first state to a second state. The first and second states of the substance differ in at least one photophysical property. The sample structure is labeled by binding a suitable protein tag including a fluorogen activating protein (FAP) to the sample structure or by expressing the protein tag and the sample structure together as a fusion protein. Then the protein tag is bound to the substance.05-20-2010
20090130773Method of Enhancing a Fluorescent Signal - A composition comprising a hydrogel particle and a fluorophore; wherein said composition produces an enhanced fluorescent signal when excited by an energy source capable of exciting the fluorophore.05-21-2009
20080280374METHODS AND SYSTEMS FOR DETECTING BIOLOGICAL AND CHEMICAL MATERIALS ON A SUBMICRON STRUCTURED SUBSTRATE - Methods and systems for detecting biological or biochemical analytes generally comprising, a metal film having one or more surfaces comprising one or more submicron structures; a device for applying one or more analytes to at least a portion of the film surface to interact with said metal film; a light source for illuminating a surface of the metal film so that at least some of the light is adapted to be optically altered by the functionalized metal film; and an optical detection subsystem for collecting the optically altered light, wherein the altered light is indicative of surface plasmon resonance on the film, and detecting one or more properties of the analytes based on the collected light.11-13-2008
20080293154Apparatus and method for detecting target - The target detecting apparatus includes: a first container having a metal inside and containing a fluorescent particle which generates fluorescence on exposure to light and quenches the fluorescence on contacting the metal; a second container having the metal inside and containing a target sample that contains at least the fluorescent particle therein; a centrifugal force giving unit configured to allow the fluorescent particles to be in contact with the metal by giving a centrifugal force to the first container and the second container; a light irradiation unit configured to expose the fluorescent particle contained in each of the first container and the second container to light; and a fluorescence detecting unit configured to detect an intensity of fluorescence generated by the fluorescent particle upon exposure to light from the light irradiation unit.11-27-2008
20080293155SALICYLAMIDE-LANTHANIDE COMPLEXES FOR USE AS LUMINESCENT MARKERS - The present invention provides luminescent lanthanide metal chelates comprising a metal ion of the lanthanide series and a complexing agent comprising at least one salicylamidyl moiety. Also provided are probes incorporating the salicylamidyl ligands of the invention and methods utilizing the ligands of the invention and probes comprising the ligands of the invention.11-27-2008
20080233658Environmental Fluorescent Sensors - The present disclosure relates to a fluorescence marker such as quantum dots and their use as sensors which may rely upon a change in fluorescence output upon exposure to a given environmental condition. The variation in fluorescence output may then be utilized as an indication of exposure to a given environmental condition.09-25-2008
20080248587Methods for Verifying Fluid Movement - Methods for using semiconductor nanocrystals for determining fluid movement, fluid dilution and fluid removal are described. Methods for using semiconductor nanocrystals for monitoring and quantifying the amounts of solid materials dissolved in a liquid are also described.10-09-2008
20090233377MICROPARTICLE-PROTEIN COMPLEX, METHOD FOR PRODUCTION OF THE COMPLEX, SEMICONDUCTOR DEVICE, AND FLUORESCENT LABELING METHOD - First, an ammonium acetate solution and an acetate solution of a metal such as Cd or Zn are mixed, so as to form an ammonium complex of the metal. Next, an apoferritin solution and thioacetic acid are added to the thus obtained reaction solution. By allowing the reaction solution to stand for 12 hours or more, a complex of a nanoparticle including CdS or ZnS and apoferritin is produced.09-17-2009
20090137058CHEMICAL AND BIOLOGICAL DETECTION METHOD AND DEVICE BASED ON MEASUREMENTS OF FLUORESCENCE AND REFLECTIVITY - A device and method for detecting the presence of one or more analytes, bound directly or indirectly to a binding substrate functionalized with a fluorophore, based on measurements of fluorescence and reflectivity. The device and methods comprise an excitation source that emits light capable of being absorbed by a fluorophore and results in the fluorophore's excitation and emission, a fluorescent probe specific for the analyte that is attached via chemisorption to the binding substrate, a detector, and a processor adapted to determine the quantity of the one or more analytes present, by correlating measurements of reflected and fluorescent light.05-28-2009
20110269243SYSTEMS AND METHODS RELATED TO OPTICAL NANOSENSORS COMPRISING PHOTOLUMINESCENT NANOSTRUCTURES - Systems and methods related to optical nanosensors comprising photoluminescent nanostructures are generally described.11-03-2011
20090162939Device for the Detection of a Fluorescent Dye - The present invention relates to a device for the detection of a fluorescent dye in a sample, comprising a radiation source means with the aid of which light for exciting the fluorescent dye to be detected can be transmitted into the sample applied to a carrier, and a detecting means for detecting fluorescent light which has been emitted by the fluorescent dye to be detected. The present invention is characterized by a hollow space having an internal high-reflectance surface, a first aperture directed towards the sample, and a second aperture located opposite the detecting means.06-25-2009
20090137060MULTICOLORED PARTICLES - A set of polymer particles stained with at least two fluorescent dyes is presented. At least 16 subsets of particles can be resolved on the basis of variable emission from the at least two fluorescent dyes where emission from at least one dye derives from a fluorescent dye covalently attached to the particle surface. All particles in the set of polymer particles can bind a uniform amount of a capture reagent. A method for the preparation of the set of polymer particles as well as a kit including the set of polymer particles is also presented as well as methods and uses of the set of polymer particles.05-28-2009
20090142853MICROFLUIDIC SYSTEM AND METHODS - A microfluidic system comprising: at least one microfluidic channel, the inner surface of which is fluorinated or fluorous; and a pump for supplying a flow of an aqueous medium containing chemical reagents or assay components to said microfluidic channel. Preferably, the apparatus further comprises a supply of a non-aqueous medium which is compatible with the surface of the microfluidic channel but immiscible with the aqueous medium, such as a perfluorocarbon solvent, for forming a sheath around the flowing aqueous medium whereby the aqueous medium is suspended away from the surface of the microfluidic channel. Also provided are methods for carrying out a chemical reaction or a biological assay in the microfluidic systems of the subject matter disclosed herein.06-04-2009
20090017554DETECTION AND MIXING IN A CONDUIT IN INTEGRATED BIOANALYSIS SYSTEMS - Apparatuses and methods in which detection is integrated with various liquid processing and environmental control functions to create integrated bioanalysis systems are disclosed. Though the various integrated bioanalysis systems are useful for any number of analysis formats, they are adaptable to high-throughput processing of samples.01-15-2009
20100248387Method for Measuring Luminescence at a Luminescence Detection Workstation - A luminescence detecting apparatus and method for analyzing luminescent samples is disclosed. Luminescent samples are placed in a plurality of sample wells in a tray, and the tray is placed in a visible-light impervious chamber containing a charge coupled device camera. The samples may be injected in the wells, and the samples may be injected with buffers and reagents, by an injector. In the chamber, light from the luminescent samples pass through a collimator, a Fresnel field lens, a filter, and a camera lens, whereupon a focused image is created by the optics on the charge-coupled device (CCD) camera. The use of a Fresnel field lens, in combination with a collimator and filter, reduces crosstalk between samples below the level attainable by the prior art. Preferred embodiments of the luminescence detecting apparatus and method disclosed include central processing control of all operations, multiple wavelength filter wheel, and robot handling of samples and reagents. Preferred embodiments of processing software integrated with the invention include elements for mechanical alignment, outlier shaving, edge detection and masking, manipulation of multiple integration times to expand the dynamic range, crosstalk correction, dark subtraction interpolation and drift correction, multi-component analysis applications specifically tailored for luminescence, and uniformity correction.09-30-2010
20100248386SEMICONDUCTOR PHOSPHOR NANOPARTICLE ASSEMBLY, PRODUCING METHOD THEREOF AND SINGLE MOLECULE OBSERVATION METHOD BY USE THEREOF - The present invention provide a semiconductor phosphor nanoparticle assembly which exhibits no variation in emission wavelength and emission intensity for every particle and is capable of achieving stable evaluation when performing a single molecule observation by using an assembly of semiconductor phosphor nanoparticles as a fluorescence labeling agent, a production method thereof and a single molecule observation method by use thereof. The production method of the semiconductor phosphor nanoparticle assembly, which is performed by a liquid phase process or a gas phase process, comprises the steps of forming nuclear particles and allowing the nuclear particles to grow or fuse, wherein a concentration by number of the formed nuclear particles is not more than a specific concentration.09-30-2010
20100248385MULTI-ACCEPTOR MOLECULAR PROBES AND APPLICATIONS THEREOF - An oligonucleotide-based molecular probe includes at least one pin loop, the pin loop including a loop sequence complementary to a target sequence. A first stem sequence is attached to one end of the pin loop, the first stem having at least one fluorescent label attached thereto. A second stem sequence is attached to the other end of the pin loop. The second stem has a plurality of quencher molecules attached thereto.09-30-2010
20090068757APPARATUS, PROCESS AND KIT FOR DETECTING ANALYTES IN A SAMPLE - The invention relates to an apparatus (03-12-2009
20090117664LIQUID SENDING METHOD OF LIQUID IN SUBSTRATE CHANNEL AND LIQUID SENDING APPARATUS - A liquid sending method includes the step of introducing either one fluid of a gas or an insulating liquid into a channel disposed on a substrate, thereby dividing a liquid flowing in the channel and sending the liquid.05-07-2009
20090221089PROBE CHIP, SENSING APPARATUS USING THE SAME AND METHOD OF DETECTING SUBSTANCES USING THE SAME - A probe chip comprises: a prism; a metal film provided on a surface of the prism and which has provided on its surface a first binding material that binds to the analyte; and a channel substrate that is provided on a side of the prism and which has formed therein a channel for supplying the liquid sample to the metal film by causing the liquid material to travel from a beginning end portion to a terminal end portion, the channel being formed in such a way that a zone from a point between the beginning end portion and the metal film to a position of contact with the metal film separates into a first branch and a second branch that has an area where a second binding material that is labeled with the fluorescent material and which binds to the analyte is placed.09-03-2009
20100015721Detection of Promiscuous Small Submicrometer Aggregates - The invention provides methods for the detection of aggregating molecules that are capable of promiscuous or non-specific binding to proteins in a time efficient manner without the use of labels.01-21-2010
20130122603Methods and Systems for Determining Composition and Completion of an Experiment - Methods and systems for determining composition and completion of an experiment are provided. One-computer-implemented method for determining composition and completion of an experiment includes determining one or more characteristics of data acquired during the experiment and determining if the experiment is completed based on the one or more characteristics. One system configured to determine composition and completion of an experiment includes a processor configured to determine one or more characteristics of data acquired during the experiment and to determine if the experiment is completed based on the one or more characteristics. Another system configured to perform an experiment includes a measurement subsystem configured to acquire data during the experiment and a processor configured to determine one or more characteristics of the data during the experiment and to determine the composition of the experiment and if the experiment is completed based on the one or more characteristics.05-16-2013
20130217143CHEMICAL-ANALYSIS DEVICE INTEGRATED WITH METALLIC-NANOFINGER DEVICE FOR CHEMICAL SENSING - A chemical-analysis device integrated with a metallic-nanofinger device for chemical sensing. The chemical-analysis device includes a metallic-nanofinger device, and a platform. The metallic-nanofinger device includes a substrate, and a plurality of nanofingers coupled with the substrate. A nanofinger of the plurality includes a flexible column, and a metallic cap coupled to an apex of the flexible column. At least the nanofinger and a second nanofinger of the plurality of nanofingers are to self-arrange into a close-packed configuration with at least one analyte molecule. A morphology of the metallic cap is to generate a shifted plasmonic-resonance peak associated with amplified luminescence from the analyte molecule. A method for using, and a chemical-analysis apparatus including the chemical-analysis device are also provided.08-22-2013
20090170214Luminescent Reporter Modality for Analyzing an Assay - An optical analysis flow system and a method of assay analysis includes a means for facilitating activation of a luminescent material coupled to particles entrained within a fluid assay, wherein the means is arranged such that the activation of the luminescent material is conducted at a site along a flow path of the fluid assay prior to an examination zone of the optical analysis flow system. The method further includes measuring luminescent light emitting from the particles as they flow through the examination zone. Another method of assay analysis includes respectively measuring different types of luminescent light emission from a first set and a second set of particles comprising a fluid assay. An optical analysis system includes at least two distinct means for respectively facilitating the activation of at least two different luminescent materials coupled to particles of a fluid assay.07-02-2009
20090148956OPTICAL DETERMINATION OF GLUCOSE UTILIZING BORONIC ACID ADDUCTS - The present invention concerns an improved optical method and optical sensing device for determining the levels of polyhydroxyl-substituted organic molecules in vitro and/or in vivo in aqueous media. The range of detection is between about 400 and 800 nm. In particular, a sensory devise is implemented in a mammal to determine sugar levels. Specifically, a dye is combined with a conjugated nitrogen-containing heterocyclic aromatic boronic acid-substituted bis-onium compound in the presence of a sugar, such as fructose or glucose. The viologens are preferred as the aromatic conjugated nitrogen-containing boronic acid substituted compounds. The method is useful to determine sugar levels in a human being.06-11-2009
20090111192BIOGENIC SUBSTANCE DETECTOR AND BIOGENIC SUBSTANCE DETECTION METHOD - A biogenic substance detector with high reaction efficiency and detection sensitivity is obtained.04-30-2009
20100297777SUB-DIFFRACTION LIMIT IMAGE RESOLUTION AND OTHER IMAGING TECHNIQUES - The present invention generally relates to sub-diffraction limit image resolution and other imaging techniques. In one aspect, the invention is directed to determining and/or imaging light from two or more entities separated by a distance less than the diffraction limit of the incident light. For example, the entities may be separated by a distance of less than about 1000 nm, or less than about 300 nm for visible light. In one set of embodiments, the entities may be selectively activatable, i.e., one entity can be activated to produce light, without activating other entities. A first entity may be activated and determined (e.g., by determining light emitted by the entity), then a second entity may be activated and determined The entities may be immobilized relative to each other and/or to a common entity. The emitted light may be used to determine the positions of the first and second entities, for example, using Gaussian fitting or other mathematical techniques, and in some cases, with sub-diffraction limit resolution. The methods may thus be used, for example, to determine the locations of two or more entities immobilized relative to a common entity, for example, a surface, or a biological entity such as DNA, a protein, a cell, a tissue, etc. The entities may also be determined with respect to time, for example, to determine a time-varying reaction. Other aspects of the invention relate to systems for sub-diffraction limit image resolution, computer programs and techniques for sub-diffraction limit image resolution, methods for promoting sub-diffraction limit image resolution, methods for producing photoswitchable entities, and the like.11-25-2010
20110111518GRANULAR NANOPARTICLES HAVING BRIGHT FLUORESCENCE AND GIANT RAMAN ENHANCEMENTS - The present invention provides nanoparticles having bright fluorescence, where the total number of photons emitted from a single nanoparticle upon excitation with an excitation wavelength of the nanoparticle is at least 107, and giant Raman enhancements, where Raman signal for a molecule near a single nanoparticle increases at least 107 times. The nanoparticles of the invention comprise a plurality of crystallites that are each about 0.6 nm to about 10 nm in size. The present invention also provides methods for making the nanoparticles, which include mixing a matrix material with a reactant capable of being thermally reduced to form the nanoparticle; forming a mixed solid phase; and thermally reducing the mixed solid phase to form the nanoparticle.05-12-2011
20100178709Magnetic Microspheres For Use In Fluorescence-Based Applications - Microspheres, populations of microspheres, and methods for forming microspheres are provided. One microsphere configured to exhibit fluorescent and magnetic properties includes a core microsphere and a magnetic material coupled to a surface of the core microsphere. About 50% or less of the surface of the core microsphere is covered by the magnetic material. The microsphere also includes a polymer layer surrounding the magnetic material and the core microsphere. One population of microspheres configured to exhibit fluorescent and magnetic properties includes two or more subsets of microspheres. The two or more subsets of microspheres are configured to exhibit different fluorescent and/or magnetic properties. Individual microspheres in the two or more subsets are configured as described above.07-15-2010
20110111520LUMINESCENT NANOSTRUCTURED MATERIALS FOR USE IN ELECTROGENERATED CHEMILUMINESCENCE - A nanostructured particulate material, which includes a redox active luminescent organic and/or ionic compound, is provided herein. The nanostructured particulate material may be used for determining the presence of an analyte of interest in a sample by detecting the emitted electromagnetic radiation generated by exposing a reagent mixture, which includes the nanostructured material and the target analyte, to chemical or electrochemical energy.05-12-2011
20090317917Biosensor and Method for Detecting Analytes - The present invention concerns in general a biosensor in the form of a microchip for the optical detection of analytes and a method using this biosensor. In particular the invention concerns biosensors for detecting an analyte by time-resolved luminescence measurement and a corresponding method.12-24-2009
20080268549THIOFLAVIN T METHOD FOR DETECTION OF AMYLOID POLYPEPTIDE FIBRIL AGGREGATION - The present invention provides a method for determining the stoichiometric ratio of the amyloid protein and the base which will result in a linear relationship between the amount of amyloid protein and the Thioflavin T fluorescence. Suitable stoichiometric ratios of the amyloid protein to base (such as 10 mM NaOH) of 1 μg:1 μl to 1 μg:1.25 μl can then be used in a Thioflavin T assay to identify potential agents which can inhibit or reduce amyloid protein fibril aggregation.10-30-2008
20100197039FLUORESCENT MATERIALS FOR HIGHLY SENSITIVE AND SELECTIVE SENSING OF AMINES AND NANOFIBRIL MATERIALS MADE THEREFROM - A fluorescence sensory material with high sensitivity, selectivity, and photostability has been developed for vapor probing of organic amines. The sensory material is a perylene-3,4,9,10-tetracarboxyl compound having amine binding groups and the following formula08-05-2010
20080206885Environmentally sensitive fluorophores - The present invention provides novel fluorophore compounds.08-28-2008
20100330693HIGH PRECISION SCANNING OF ENCODED HYDROGEL MICROPARTICLES - Techniques are provided for high precision scanning of hydrogel microparticles. The high precision is achieved by one or more modifications to the microparticle composition, or microfluidics apparatus that align the microparticles in a detection channel, or method of preparing a sample for introduction into the apparatus, or some combination. An apparatus comprises a body structure having formed therein a central channel and multiple focusing channels in fluid communication with the central channel through multiple junctions. A width of the central channel is smaller in a portion downstream of each junction. A particle comprises a hydrogel matrix and a probe molecule. The particle has an aspect ratio greater than about three. A method includes loading into a sample fluid inlet a mixture, wherein a number of particles lies within a range from about 15 to about 20 particles/μl.12-30-2010
20100330694 BROMATE ION MEASUREMENT METHOD AND APPARATUS - A method for measuring bromate ion is provided that provides high-sensitivity measurement results more simply and more quickly than conventional bromate ion measurement methods. A fluorescent substance that is quenched by coexistence with bromate ions is added to a sample and the fluorescence intensity of the fluorescent substance after quenching is measured, the measured fluorescence intensity being subtracted from the fluorescence intensity of a standard sample containing no bromate ions to calculated the fluorescence intensity difference. The bromate ion concentration is calculated from the calculated fluorescence intensity difference, using a pre-determined calibration line between the fluorescence intensity difference and the bromate ion concentration.12-30-2010
20100167413METHODS AND SYSTEMS FOR ANALYZING FLUORESCENT MATERIALS WITH REDUCED AUTOFLUORESCENCE - Mitigative and remedial approaches to reduction of autofluorescence background noise are applied in analytical systems that rely upon sensitive measurement of fluorescent signals from arrays of fluorescent signal sources. Such systems are for particular use in fluorescence based sequencing by incorporation systems that rely upon small numbers or individual fluorescent molecules in detecting incorporation of nucleotides in primer extension reactions. Systems and methods for analyzing highly multiplexed sample arrays using highly multiplexed, high-density optical systems to illuminate high-density sample arrays and/or provide detection and preferably confocal detection off signals emanating from such high-density arrays. Systems and methods are applied in a variety of different analytical operations, including analysis of biological and biochemical reactions, including nucleic acid synthesis and derivation of sequence information from such synthesis.07-01-2010
20120309103METHOD FOR MEASURING LUMINESCENCE AT A LUMINESCENCE DETECTION WORKSTATION - A luminescence detecting apparatus and method for analyzing luminescent samples is disclosed. Luminescent samples are placed in a plurality of sample wells in a tray, and the tray is placed in a visible-light impervious chamber containing a charge coupled device camera. The samples may be injected in the wells, and the samples may be injected with buffers and reagents, by an injector. In the chamber, light from the luminescent samples pass through a collimator, a Fresnel field lens, a filter, and a camera lens, whereupon a focused image is created by the optics on the charge-coupled device (CCD) camera. The use of a Fresnel field lens, in combination with a collimator and filter, reduces crosstalk between samples below the level attainable by the prior art. Preferred embodiments of the luminescence detecting apparatus and method disclosed include central processing control of all operations, multiple wavelength filter wheel, and robot handling of samples and reagents. Preferred embodiments of processing software integrated with the invention include elements for mechanical alignment, outlier shaving, edge detection and masking, manipulation of multiple integration times to expand the dynamic range, crosstalk correction, dark subtraction interpolation and drift correction, multi-component analysis applications specifically tailored for luminescence, and uniformity correction.12-06-2012
20110189787SPECTROMETER AND METHOD OF OPERATING A SPECTROMETER - A photoluminescence spectrometer (08-04-2011
20100022023Pressure activatable chemiluminescent system useful for covert intrusion detection - This invention is directed towards chemiluminescent systems useful in the practice of methods of intrusion detection, compositions useful for intrusion detection and methods for their formation, and to covert intrusion detection techniques and compositions therefore containing an infrared light generating chemiluminescent system, blended into an earthen-like carrier that enable one to detect whether any movement has breached an area during a certain time frame.01-28-2010
20100022022FLUOROGENIC HOMOGENEOUS BINDING ASSAY METHODS AND COMPOSITIONS - Disclosed are binding substrate compositions, methods and kits useful for, among other things, detecting and/or characterizing binding interactions between molecules of interest.01-28-2010
20100022021NEW AZIDE SUBSTITUTED NAPHTHYLENE OR RYLENE IMIDE DERIVATIVES AND THEIR USE AS REAGENTS IN CLICK-REACTIONS - Novel mono-azide substituted rylene-imide derivatives, their use in methods for the detection of analytes and reagents kits for the detection of analytes comprising said novel mono-azide substituted rylene-imide derivatives.01-28-2010
20090068756METHODS FOR DETERMINING ORGANIC BIOCIDE CONCENTRATION IN A COMPOSITE WOOD PRODUCT - A composite wood product and methods for manufacturing the same and determining the concentration and distribution of an organic biocide within a composite wood product are provided. The organic biocide may be added to wood elements (i.e., fibers, flakes, strands, veneers) prior to consolidation and/or heating of the wood particles to form the composite wood product. A tracer additive may be mixed with the biocide, or applied separately to the furnish which is used to produce the composite wood product. The tracer additive may be detected via, for example, x-ray fluorescence. An amount of tracer additive detected may correlate to an amount of organic biocide within the wood elements and/or the composite wood product.03-12-2009
20110117666FLUORESCENT PROBE - A fluorescent probe comprising a compound represented as (Fluorophore A)-S-(Fluorophore B) (Fluorophore A and Fluorophore B are fluorophores which emit fluorescence when they are irradiated with an excitation light of a wavelength of 600 to 950 nm, Fluorophore A has a property that it shows change of fluorescence characteristic before and after a specific reaction with an substance to be measured, and S represents a spacer which connects Fluorophore A and Fluorophore B), which compound shows substantial change in efficiency of fluorescence resonance energy transfer between Fluorophore A and Fluorophore B before and after the specific reaction with the objective substance, wherein Fluorophore A is represented by the following formula (AI):05-19-2011
20110117667PHOTOINDUCED SIGNAL AMPLIFICATION THROUGH EXTERNALLY SENSITIZED PHOTOFRAGMENTATION IN MASKED PHOTOSENSITIZERS AND PHOTOAMPLIFIED FLUORESCENCE TURN-OFF SYSTEM - Provided is a photoamplified fluorescence turn-off assay where a masked photosensitizer is mixed with a fluorescent molecule. This mixture is brightly fluorescent because the masked photosensitizer is not capable of quenching the fluorophore. When the photosensitizer is released and amplified, the photosensitizer quenches the emission of fluorophores very efficiently. 05-19-2011
20110306146Parallel Screening Supercritical Fluid Chromatography - The invention provides an apparatus for supercritical fluid chromatography. The apparatus comprises a binary pump; an autosampler; a sampling valve; a first and second port switching valve; a first and second manifold; two or more channels, each having a check valve assembly, a separation column and one or more detectors operatively connected thereon; and a backpressure regulator. The apparatus also includes computer software and hardware to control distribution of fluid through the apparatus, including switching between a multi-channel mode or a single channel ode; 2) analyze data collected by the one or more detectors; and 3) optimize separation of analytes by controlling solvent combinations, concentration gradients, pressure and temperature. The apparatus excludes additional backpressure regulators or pumps on individual channels. Also provided is a method of screening a sample, using supercritical chromatography, using the above apparatus, where multiple samples can be screened simultaneously with parallel processing.12-15-2011
20110306145METHOD AND DEVICE FOR FABRICATING AEROGELS AND AEROGEL MONOLITHS OBTAINED THEREBY - Method and devices for rapidly fabricating monolithic aerogels, including aerogels containing chemical sensing agents, are disclosed. The method involves providing a gel precursor solution or a pre-formed gel in a sealed vessel with the gel or gel precursor at least partially filling the internal volume of the vessel and the sealed vessel being positioned between opposed plates of a hot press; heating and applying a restraining force to the sealed vessel via the hot press plates (where the restraining force is sufficient to minimize substantial venting of the vessel); and then controllably releasing the applied restraining force under conditions effective to form the aerogel. A preferred device for practicing the method is in the form of a hot press having upper and lower press plates, and a mold positioned between the upper and lower plates. Doped aerogel monoliths and their use as chemical sensors are also described.12-15-2011
20090176313Fluorescent Compound and Labeling Agent Comprising the Same - A novel fluorescent compound having a high light fastness, high fluorescence quantum yield and sharp absorption spectrum, which emits fluorescence having a wavelength in long wavelength region, as well as its use as a labeling agent, is disclosed. In Formula [I] below, by forming a specific hetero ring(s) with R07-09-2009
20120301971FLUORESCENT PARTICLE, WITH SEMICONDUCTOR NANOPARTICLES DISPERSED THEREIN, FABRICATED BY THE SOL-GEL PROCESS - An object of the present invention is to prepare a fine particle with high durability and high brightness, in which semiconductor nanoparticles are assembled. The present invention provides fluorescent fine particles comprising Cd- and Se-containing semiconductor nanoparticles dispersed in silicon-containing fine particles, wherein the average particle size of the silicon-containing fine particles is 20 to 100 nm, and the number of semiconductor nanoparticles dispersed in the silicon-containing fine particles is 10 or more.11-29-2012
20110111519Reagent for detecting an analyte - A reagent for use in detecting an analyte comprises a fluorescent energy donor and an energy acceptor, wherein the energy acceptor is of the general formula:05-12-2011
20120040471Zn4(OH)2(1,2,4-BTC)2 - a rod packing microporous metal-organic framework with open metal sites for selective separation and sensing of small molecules - Disclosed herein are rod-packing robust microporous metal-organic frameworks having the repeat unit Zn02-16-2012
20120045846SYSTEM AND METHOD FOR pH FORMULATIONS - A system and method for creating a buffer solution having a desired pH value is disclosed. The method uses two known buffer solutions, each with predetermined pH values, and determines a mathematical relationship which defines the amount of each known buffer solution needed to create the buffer solution with the desired pH. This method can then be used to create one or more denaturation graphs, which demonstrate the stability of a protein at a given pH level.02-23-2012
20090023223PLUG FLOW SYSTEM FOR IDENTIFICATION AND AUTHENTICATION OF MARKERS - Devices and methods for extraction, identification, authentication, and quantification of one or more covert markers in a material are disclosed. An extraction system includes a first plug flow mixer for mixing a first fluid bearing a marker and transfer agent into a plug flow. The mixing and flowing of the immiscible liquids causes transfer of the marker from the fluid to the transfer agent. A splitter having filters of different surface energies separates the two immiscible liquids, the transfer agent bearing the marker. A second plug flow can be used to transfer the marker to a second transfer agent. The transferred marker is detected to authenticate the original fluid. The marker can be further isolated, activated, or reacted to perform detection, identification or authentication. With the device, a number of independent processing and analytic steps are combined onto a single, portable unit.01-22-2009
20090298193ATOMIC EMISSION SPECTROSCOPY ON A CHIP - A method of inducing explosive atomization of materials is provided using a metal-oxide-semiconductor (MOS)-based structure under electrical excitation. Explosive atomization of the gate electrode and surrounding dielectric materials creates a microplasma that is substantially confined with the device at the metal/dielectric interface. The device can generate a microplasma in either the accumulation or inversion regime. The high degree of confinement of the microplasma allows chip-scale implementation of atomic emission spectroscopy and detection using a minimal amount of analyte.12-03-2009
20080299674Novel Isonitrile Compound, Its Method Of Manufacture, A Marine Fouling Organism Larva Fluorescent, And A Method Of Detecting This Marine Fouling Organism Larva Using Said Marker - Novel isonitrile compounds represented by the general formula F-A-NC, their method of manufacture, a marine fouling organism larva fluorescent marker having at least one of these compounds as an active ingredient, and a method of detecting this marine fouling organism larva using this marker.12-04-2008
20120156797Luminescent nanochannel sensors - In a nanochannel thin film in which oxide layers have surfactant micelles therein, the presence of a target substance in a sample solution is detected with a luminescence intensity of a thin film provided by recognition of the target substance with a luminescent recognition reagent in the nanochannels. Upon focusing on a hydrophobic field provided by the presence of the surfactant in pores of a nanometer size, the novel development of a sensor function is enabled.06-21-2012
20090017552FLUORESCEIN BASED SENSORS FOR TRACKING NITRIC OXIDE IN LIVE CELLS - The present invention is directed, in part, to coordination complexes for detecting analytes, and methods of making and using the same.01-15-2009
20110104815CONJUGATED NANOPARTICLES AND THEIR USE IN DETECTION OF LATENT FINGERPRINTS - Novel conjugates of nanoparticles are provided and have particular utility in the detection of latent fingerprints by their ability to bind to a fingerprint residue. The conjugate comprises a nanoparticle attached to a linker group having a terminal reactive moiety, wherein said nanoparticle comprises a core of a first semiconductor material having a first luminescence and a shell of a second material which at least partially surrounds the core. The conjugated nanoparticle can bind to the fingerprint residue and can be detected using fluorescence.05-05-2011
20090130772Apparatus and method for collecting data on light-emitting reactions - The invention concerns an apparatus and a method for collecting data on a set of light-emitting molecular reactions. The apparatus comprises a holder for a reaction vessel comprising a plurality of reaction spaces containing first reaction substance, feeder for supplying second reaction substance to said reaction spaces for initiating said reaction set, and a plurality of light detectors for measuring light emitted from said reaction spaces in synchronized relationship with said feeding. According to the invention, at least two of said light detectors are arranged on opposing sides of the reaction vessel and being adapted to simultaneously measure different properties of the reaction set, that is, different wells or wavelengths, for example. The invention allows doubling the measurement speed in aequorin-based intracellular Ca-measurements.05-21-2009
20100210030ASSEMBLY OF SEMICONDUCTOR NANOPARTICLE PHOSPHORS, PREPARATION METHOD OF THE SAME AND SINGLE-MOLECULE OBSERVATION METHOD USING THE SAME - Disclosed are an assembly of semiconductor nanoparticle phosphors, which can provide stable evaluation without variation in emission wavelength or in intensity of emission among the particles when used as a labeling agent through which a single-molecule observation is carried out, a preparation method of the assembly, and a single-molecule observation method employing the assembly. Also disclosed is a method for preparing an assembly of semiconductor nanoparticle phosphors according to a liquid phase method, the method comprising the step of reacting a semiconductor precursor at a temperature which is not lower than the melting point of the semiconductor precursor and is not higher than the boiling point of a solvent.08-19-2010
20120214253MACROCYCLIC HOPO CHELATORS - The present invention provides a novel class of macrocyclic compounds as well as complexes formed between a metal (e.g., lanthanide) ion and the compounds of the invention. Preferred complexes exhibit high stability as well as high quantum yields of lanthanide ion luminescence in aqueous media without the need for secondary activating agents. Preferred compounds comprise hydroxypyridinonyl moieties within their macrocyclic structure and are characterized by surprisingly low, non-specific binding to a variety of polypeptides such as antibodies and proteins as well as high kinetic stability.08-23-2012
20120135537DETECTION OF A COMPONENT OF INTEREST WITH AN ULTRAVIOLET LASER AND METHOD OF USING THE SAME - Means and method for analysis of a component of interest in a sample is provided. A small sample cell is provided which decreased dispersion of the sample. Also provided is a device for detection of a component of interest in a sample in which the small sample cell is used with an ultra violet laser in which the energy of the laser is spread over an area such that energy density is above desorption threshold, but the sample not ablated. Rapid and reliable detection of a component of interest is provided with the invention.05-31-2012
20120252132VARIOUS-SUBSTANCE HOLDER, VARIOUS-SUBSTANCE HOLDER TREATING APPARATUS, AND VARIOUS-SUBSTANCE HOLDER TREATING METHOD - A various-substance holder, a various-substance holder treating apparatus, and a various-substance holder treating method are provided which enable the mutual identification of particulate carriers to which various substances are or can be immobilized without the need to arrange the particulate carriers at predetermined positions or in a predetermined order, eliminating the need for time and effort to arrange the various substances at predetermined positions or in a predetermined order to allow treatments to be quickly and easily achieved. The various-substance holder has a plurality of particulate carriers or plural sets of particulate carriers to which plural types of chemical substances are or can be immobilized and a carrier holding portion holding the plurality of particulate carriers or the plural sets of particulate carriers in a substantially stationary state such that the plurality of particulate carriers or the plural sets of particulate carriers can be externally measured.10-04-2012
20120171776 METHOD FOR DETECTING AN ANALYTE - The disclosure relates to a method of detecting analytes using non-aromatic dendritic macromolecules. The inherent photoluminescence of dendrimers are utilized to detect analytes which are electron deficient in nature.07-05-2012
20120077282LUMINESENCE MEASUREMENT UTILIZING CARTRIDGE WITH INTEGRATED DETECTOR - A cartridge and cartridge system for use in an apparatus for analyzing a sample are provided. The system has a plurality of cartridges for different applications for a multimode instrument. The cartridges are removably engaged with a cartridge support of the apparatus in a “plug-in” format such that one cartridge may be removed from the apparatus and another cartridge may be easily installed. The cartridge support includes a plurality of cartridge positions that receive cartridges concurrently. One of the cartridges may be a luminescence cartridge that includes an integrated detector that is movable toward and away from a sample carrier of the apparatus, and thus toward and away from a sample located at the sample carrier.03-29-2012
20100047919BIOSENSOR DEVICE AND METHOD FOR DETECTING MOLECULES IN AN ANALYTE - A biosensor device (02-25-2010
20120178177Biological Components Within the Cerebrospinal Fluid - The invention provides novel methods for isolating, characterizing, comparing, and using biological components that are present in the cerebrospinal fluid. Such biological structures, called CS-MPs, can be used for identifying biomarkers that reflect the status (or anticipate the development) of disorders of the Central nervous System (CNS). The novel methods, biological products, and related kits make possible the use of CS-MPs and of their components as biomarkers for the diagnosis, prognosis, or monitoring of CNS disorders. The CS-MPs have a diameter comprised between 100 and 1000 nm and contain phosphatidylserine (PS).07-12-2012
20100291703BIOSENSOR - A biosensor applicable to an environment suitable for biosensing is provided, which is a solid-state element for performing detections in an aqueous environment. The biosensor at least includes a biosensing layer, a light-emitting diode and a photodiode. The biosensing layer causes changes in the light-emitting property thereof after absorbing, adsorbing and/or bonding with a biological substance released during in vivo signal transduction in an organism, and the rays of light generated by excitation of the light-emitting diode causes the biosensing layer to emit fluorescence. After the fluorescence is absorbed by the photodiode, it can be converted into an interpretable photocurrent signal. Afterwards, the meaning of the in vivo signal transduction can be understood by interpretation of the photocurrent signal.11-18-2010
20100291702Functionalized Fluorescent Nanocrystal Compositions and Methods for Their Preparation - The present invention provides for functionalized fluorescent nanocrystal compositions and methods for making these compositions. The compositions are fluorescent nanocrystals coated with at least one material. The coating material has chemical compounds or ligands with functional groups or moieties with conjugated electrons and moieties for imparting solubility to coated fluorescent nanocrystals in aqueous solutions. The coating material provides for functionalized fluorescent nanocrystal compositions which are water soluble, chemically stable, and emit light with a high quantum yield and/or luminescence efficiency when excited with light. The coating material may also have chemical compounds or ligands with moieties for bonding to target molecules and cells as well as moieties for cross-linking the coating. In the presence of reagents suitable for reacting to form capping layers, the compounds in the coating may form a capping layer on the fluorescent nanocrystal with the coating compounds operably bonded to the capping layer.11-18-2010
20120258548REDOX SENSOR - Provided herein are devices, methods, and uses for measuring redox potential. For example, provided herein are fiber optic redox sensors and methods of use thereof.10-11-2012
20120190125WATER-SOLUBLE SILSESQUIOXANES AS ORGANIC QUANTUM DOTS FOR SENSING AND IMAGING - This invention includes a compound represented by the following structural formula (I) or an acceptable salt thereof.07-26-2012
20120264226OPTICAL LENS SYSTEM AND METHOD FOR MICROFLUIDIC DEVICES - An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.10-18-2012
20090017553Immunoassay-based microsensing using optical sensors - A biosensor device, system, and method for detecting biological material. The sensor includes a substrate including sample regions having attachable thereon an immobilized first species associated with the biological material and includes at least one optical sensor associated with the sample regions and configured to detect induced radiation from a second species selectively attached to the first species at the sample regions. The induced radiation provides an indication that the biological material is on the substrate. The system includes a processor in communication with the optical sensor and is configured to monitor the induced radiation from the second species. The method immobilizes a first species of the biological material on the at least one sample region, attaches a second species of the biological material to the first species, induces radiation from the second species, and detects the radiation with at least one optical sensor associated with the at least one sample region.01-15-2009
20110124116MULTI-ARRAY, MULTI-SPECIFIC ELECTROCHEMILUMINESCENCE TESTING - Materials and methods are provided for producing patterned multi-array, multi-specific surfaces for use in diagnostics. The invention provides for electrochemiluminescence methods for detecting or measuring an analyte of interest. It also provides for novel electrodes for ECL assays. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use multiply specific testing procedures.05-26-2011
20080299675Luminescent Lanthanide Binding Chelates - Lanthanide chelates derived from diazacrown ethers having two ethyliminodiacetic acid side chains have increased ability to bind lanthanide ions.12-04-2008
20110003398ASSAY DEVICE COMPRISING SERIAL REACTION ZONES - An analysis device having at least one sample addition zone, at least one sink, and at least one flow path connecting the at least one sample addition zone and the at least one sink. The at least one flow path includes projections substantially vertical to the surface of the substrate and having a height (H), diameter (D) and reciprocal spacing (t01-06-2011
20110003397Solvatochromic functional monomer and the use thereof for chemosensing by solvatochromic molecular imprinting - The present invention relates to a solvatochromic functional monomer having the chemical structure as shown in the accompanying drawing (FIG. 01-06-2011
20120322162GENERATION OF MONODISPERSE DROPLETS BY SHAPE-INDUCED SHEAR AND INTERFACIAL CONTROLLED FUSION OF INDIVIDUAL DROPLETS ON-DEMAND - A microfluidic device for generation of monodisperse droplets and initiating a chemical reaction is provided. The microfluidic device includes a first input microchannel having a first dimension and including a first phase located therein. The device also includes a second input microchannel having a second dimension and including a second phase located therein. In accordance with the present disclosure, the second dimension is different from the first dimension and the first phase is immiscible in the second phase. A microchannel junction is also present and is in communication with the first input microchannel and the second input microchannel. The device further includes an output channel in communication with the microchannel junction and set to receive a monodisperse droplet. In the present disclosure, the difference in the first dimension and the second dimension creates an interfacial tension induced force at the microchannel junction which forms the monodisperse droplet.12-20-2012
20110236989ANTIOXIDANT PROTECTION OF A CHEMICAL SENSOR - Analyte sensors having antioxidant protection are disclosed. By combining antioxidant and/or scavenger agents into polymer matrices that contain sensor moieties, the sensor moieties are protected from reactive oxygen species. Also disclosed are methods of making analyte sensors and methods of inhibiting oxidative degradation of sensing components in hydrated, polymerized analyte sensor systems.09-29-2011
20100216252Lanthanide Complexes, Preparation and Uses Thereof - The invention relates to compounds, to the complexes they form with a lanthanide, and to the use of the complexes for fluorescence marking or NMR imaging.08-26-2010
20120329171DEVICE AND METHOD FOR PRESSURE-DRIVEN PLUG TRANSPORT - The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.12-27-2012
20110159605Surface Grafted Conjugated Polymers - A surface grafted conjugated polyelectrolyte (CPE) is formed by coupling a CPE by a coupling moiety to the surface of a substrate. The substrate can be of any shape and size, and for many uses of the surface grafted CPE, it is advantageous that the substrate is a nanoparticle or microparticle. Surface grafted CPEs are presented that use silica particles as the substrate, where a modified silane coupling agent connects the surface to the CPE by a series of covalent bonds. Two methods of preparing the surface grafted CPEs are presented. One method involves the inclusion of the surface being modified by the coupling agent and condensed with monomers that form the CPE in a grafted state to the substrate. A second method involves the formation of a CPE with terminal groups that are complimentary to functionality that has been placed on the surface of the substrate by reaction with a coupling agent. The surface grafted CPEs are also described for use as biosensors and biocides.06-30-2011
20080213915SYSTEM AND METHOD FOR THE MEASUREMENT OF MULTIPLE FLUORESCENCE EMISSIONS IN A FLOW CYTOMETRY SYSTEM - A system and method for the measurement of multiple fluorescence emissions in a flow cytometry system is disclosed where each excitation light source is modulated with a different frequency. A single detector is used to collect the fluorescent emissions excited by all light sources, and the emissions are segregated using Fourier Transform techniques. Systems and methods for the correction of inter-beam coincidence are also disclosed.09-04-2008
20130095573Magnetic Microspheres For Use In Fluorescence-Based Applications - Microspheres, populations of microspheres, and methods for forming microspheres are provided. One microsphere configured to exhibit fluorescent and magnetic properties includes a core microsphere and a magnetic material coupled to a surface of the core microsphere. About 50% or less of the surface of the core microsphere is covered by the magnetic material. The microsphere also includes a polymer layer surrounding the magnetic material and the core microsphere. One population of microspheres configured to exhibit fluorescent and magnetic properties includes two or more subsets of microspheres. The two or more subsets of microspheres are configured to exhibit different fluorescent and/or magnetic properties. Individual microspheres in the two or more subsets are configured as described above.04-18-2013
20130115710TUNING OF METAL ENHANCED EMISSIONS OF LONG-LIVED LUMINESCENT COMPOUNDS - The present invention provides for the surface plasmon-enhancement of long lived luminescent compounds, thereby providing for methods and systems having enhanced and controllable rates of the radiative emission of such relaxation of long lived luminescent compounds. The present invention achieves acceleration of the radiative processes by the interaction of the long lived luminescent compounds with surface plasmons of the metal surfaces.05-09-2013
20130102087DEVICE, SYSTEM AND METHOD FOR RAPID DETERMINATION OF A MEDICAL CONDITION - Provided is a system and method for determination of a medical condition, the system including a disposable cartridge adapted to receive a volume of a body fluid, the cartridge comprising a plurality of sections, at least one of the sections adapted to react at least one reactant with the bodily fluid to form a pretreated sample; and an optics unit comprising at least one excitation illumination adapted to convey radiation to the pre-treated sample, at least one multi-spectral emission detector and at least one of a photon counter and an integrator, wherein the at least one excitation illumination and the at least one multi-spectral emission detector are disposed on the same side of the cartridge; and wherein the optics unit is adapted to detect a plurality of spectrally distinct signals generated by interaction of the radiation and the pre-treated sample in the cartridge, thereby determining said medical condition.04-25-2013
20130102088Fluorescent Sensor and Methods - Binding an analyte can cause a change in fluorescence emission of a sensor. The change in fluorescence can be related to the amount of analyte present. The sensor can include a semiconductor nanocrystal linked to a fluorescent moiety. Upon excitation, the fluorescent moiety can transfer energy to the semiconductor nanocrystal, or vice versa.04-25-2013
20100279428OPTICAL SENSING MEMBRANES, DEVICES AND METHODS FOR SIMULTANEOUS DETECTION OF TWO OR MORE PARAMETERS OF DISSOLVED OXYGEN CONCENTRATION, pH AND TEMPERATURE - Provided is an optical sensing membrane, including a mixture of two or more fluorescent dyes for detection of dissolved oxygen concentration, pH and temperature, immobilized on a support, a detection device including the optical sensing membrane and a detection method using the detection device.11-04-2010
20120258547METHOD OF MONITORING MACROSTICKIES IN A RECYCLING AND PAPER OR TISSUE MAKING PROCESS INVOLVING RECYCLED PULP - A challenge in using recycled material in the papermaking process is the presence of hydrophobic organics with adhesive properties commonly known as “stickies.” Hydrophobic agglomerates can result in spots or defects in the final paper product or deposit on papermaking equipment resulting in poor runnability and downtime. Technologies for monitoring and controlling microstickies exist. However, a need exists for a technique to rapidly determine the size and content of macrostickies (diameter>100 microns) in recycled pulp process streams. The present invention is a device and method to perform real-time macrostickies and/or any visible hydrophobic particle analysis in an aqueous medium. Using the present invention, furnish quality can be monitored and treatment performance can be monitored and controlled. The technique is based on fluorescence image analysis to identify and count sticky particles as well as measure their size.10-11-2012
20120282706PREPARATION OF AN OPTICAL PH SENSOR BASED ON FLUORESCEIN AND 1-HEPTANESULFONIC ACID SODIUM CO-INTERCALATED LAYERED DOUBLE HYDROXIDE - This invention relates to the field of preparation technology of optical pH sensor by co-intercalated fluorescein and 1-heptanesulfonic acid sodium into layered double hydroxide. The sensor is composed by conductive materials and the surface LDH films by co-interacted FLU and HES. The synthesis method is: first: synthesis of LDH colloid suspension, subsequently, the FLU and HES co-intercalated LDH colloid solution was prepared following the ion-exchange method, then the thin film of FLU-HES/LDH was spreaded on the surface of the conductive material by electrophoretic deposition, and the oriental pH sensor was synthesized. The advantages of the present invention is: first, the LDH matrix provides chromophore molecules with a confined and stable environment; the novel electrophoretic deposition strategy in this work provides a method for precise control of thickness (ranging from nanometers to micrometers), and the oriental pH sensor show good pH responsive.11-08-2012
20130122604PRELIMINARY DIAGNOSTIC SYSTEM - A preliminary diagnostic system comprising a reading station and at least one card comprising an integrated circuit and adapted to be read by the reading station, the integrated circuit of the card comprising a hydraulic circuit and a detection circuit, the card containing a reagent which is adapted to react to the presence of a marker of a disease, the hydraulic circuit being adapted to receive a sample of a biological liquid and to introduce the reagent in the sample, the detection circuit being adapted to detect the presence of the marker in the sample, the absence of the marker of the disease indicating the absence of the disease in the sample.05-16-2013
20130189793Stable Colloidal Suspensions Of Gold Nanoconjugates And The Method For Preparing The Same - In the present invention, a method for determining the stability threshold amount of a stabilizer component for gold nanoparticles to prevent their aggregation in any electrolyte solution, is disclosed. The method permits for very low levels of stabilizer components to be used while still permitting conjugation with other functional ligands. The method comprises preparation of stable gold nanoparticles conjugated with different amount of stabilizing agents in deionized water first and then testing the stability of colloidal suspension of these gold nanoparticles in the presence of the electrolyte solution by monitoring the absorbance at 520 nm. The invention also comprises a method for fabrication of nanoconjugates comprising gold nanoparticles and only the stabilizer components or comprising gold nanoparticles, stabilizer components and functional ligands, which are stable in the presence of electrolytes.07-25-2013
20120015446Chemiluminescent Compositions, Enhancing Reagents for Chemiluminescence and Methods for the Preparation and Use Thereof - A enhancing reagent for enhancing chemiluminescence of 1,2-dioxetane compounds and a method for using the enhancing reagent to enhance the chemiluminescence are provided, in which the enhancing reagent contains an alkyl bis-quaternary ammonium salt of Formula I. A chemiluminescent composition with a 1,2-dioxetane compound as a substrate and a kit thereof are further provided, which contain a 1,2-dioxetane compound and an alkyl bis-quaternary ammonium salt of Formula I.01-19-2012
20120015445Portable Fluorimetric Apparatus, Method and System - A fluorimetric system, apparatus and method are disclosed to measure an analyte concentration in a sample. A sensor reagent housing is also disclosed which comprises a channel and a porous medium having an analysis chemistry reagent, such as a nucleic acid analysis chemistry reagent. The apparatus and system are portable for field use, with an apparatus housing having a size adapted to fit into a user's hand. An apparatus also includes a sensing chamber and cover, a user interface, a light source, a photomultiplier tube, an amplifier, an A/D converter, a memory, and a processor. In various embodiments, the processor performs a data fitting, determines a sample reaction rate parameter, and determines the analyte concentration from a comparison of the sample reaction rate parameter with stored calibration data. The processor may also generate the calibration data and site-specific offset factors, and modify the calibration data using an offset factor.01-19-2012
20120028366FLOW CYTOMETER AND FLUIDIC LINE ASSEMBLY WITH MULTIPLE INJECTION NEEDLES - A flow cytometer is provided which includes an interrogation flow cell and a plurality of assay fluidic lines extending into the interrogation flow cell. A method of operating such a flow cytometer includes priming the interrogation flow cell with a sheath fluid and injecting different assay fluids into a flow of the sheath fluid through the plurality of fluidic lines. A fluidic line assembly is provided which includes a plurality of capillary tubes coupled to a base section configured for coupling to an interrogation flow cell assembly of a flow cytometer. The capillary tubes are dimensionally configured such that when the fluidic line assembly is arranged within the flow cytometer and fluid is dispensed from one or more of the capillary tubes at a given pressure differential with respect to an encompassing sheath fluid within the interrogation flow cell the fluid is substantially centrally aligned within the interrogation flow cell.02-02-2012
20090221087Safe biodegradable fluorescent particles - A human-safe fluorescence particle that can be used for fluorescence detection instruments or act as a safe simulant for mimicking the fluorescence properties of microorganisms. The particle comprises a non-biological carrier and natural fluorophores encapsulated in the non-biological carrier. By doping biodegradable-polymer drug delivery microspheres with natural or synthetic fluorophores, the desired fluorescence can be attained or biological organisms can be simulated without the associated risks and logistical difficulties of live microorganisms.09-03-2009

Patent applications in class With fluorescence or luminescence